RchiOBHm_Chr2g0122961

DNA replication ATP-dependent helicase nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0002244
Physical Location & Seq
Forward (+)
36089385 .. 36090753
1369 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49530

Sequence Viewer

Length: 234 bp
ATGTATAAGGTTCTTCGCTTATTGAATGAGCAAAGTGGAGAAGAGTGTGCTGTGTATTTATGGGTAGAGTGGTTTTGCAGTGTCATTGCACCTGGAGATACTGTCAATGTTATTGGTGAATTTGATGACCAAGGACAGTGTCATGTGGATAATCACCATAAGTTTTTAATTGTTCACCCTGATGACTTAGTGTCTGGAACTCGGGTTGCAGCTAATTTCATTTGTCATAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 map03030
Pfam Domains
Protein Families
AAA_11 AAA_12 Cas_Cas4 Dna2

Protein Analysis

77

Amino Acids

8.79

Weight (kDa)

5.06

Isoelectric Point (pI)

34.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dna2 PF08696 10 - 77 3e-19 DNA replication factor Dna2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 119
AgsI TTSAA 1 cut(s) 25
AhdI GACNNNNNGTC 1 cut(s) 190
AjnI CCWGG 1 cut(s) 91
AluBI AGCT 1 cut(s) 212
AluI AGCT 1 cut(s) 212
Ama87I CYCGRG 1 cut(s) 201
ApeKI GCWGC 1 cut(s) 209
ApoI RAATTY 1 cut(s) 119
AsuHPI GGTGA 3 cut(s) 128, 146, 167
AvaI CYCGRG 1 cut(s) 201
BbvI GCAGC 1 cut(s) 221
BciT130I CCWGG 1 cut(s) 93
BisI GCNGC 1 cut(s) 210
BlsI GCNGC 1 cut(s) 211
Bme1390I CCNGG 1 cut(s) 93
BmeRI GACNNNNNGTC 1 cut(s) 190
BmeT110I CYCGRG 1 cut(s) 201
BmrFI CCNGG 1 cut(s) 93
BpmI CTGGAG 1 cut(s) 114
BsaJI CCNNGG 1 cut(s) 130
BsaXI ACNNNNNCTCC 2 cut(s) 87, 117
Bse3DI GCAATG 1 cut(s) 84
BseBI CCWGG 1 cut(s) 93
BseDI CCNNGG 1 cut(s) 130
BseMI GCAATG 1 cut(s) 84
BseXI GCAGC 1 cut(s) 221
BsiHKCI CYCGRG 1 cut(s) 201
BsoBI CYCGRG 1 cut(s) 201
BsrDI GCAATG 1 cut(s) 84
BssECI CCNNGG 1 cut(s) 130
BssT1I CCWWGG 1 cut(s) 130
Bst2UI CCWGG 1 cut(s) 93
Bst4CI ACNGT 2 cut(s) 103, 138
Bst6I CTCTTC 1 cut(s) 36
BstDEI CTNAG 1 cut(s) 187
BstNI CCWGG 1 cut(s) 93
BstSCI CCNGG 1 cut(s) 91
BstV1I GCAGC 1 cut(s) 221
BtsI GCAGTG 1 cut(s) 85
BtsIMutI CAGTG 2 cut(s) 85, 143
CviAII CATG 1 cut(s) 143
CviJI RGCY 1 cut(s) 212
CviKI_1 RGCY 1 cut(s) 212
DdeI CTNAG 1 cut(s) 187
DriI GACNNNNNGTC 1 cut(s) 190
Eam1104I CTCTTC 1 cut(s) 36
Eam1105I GACNNNNNGTC 1 cut(s) 190
EarI CTCTTC 1 cut(s) 36
Eco130I CCWWGG 1 cut(s) 130
Eco88I CYCGRG 1 cut(s) 201
EcoRII CCWGG 1 cut(s) 91
EcoT14I CCWWGG 1 cut(s) 130
ErhI CCWWGG 1 cut(s) 130
FaeI CATG 1 cut(s) 146
FaiI YATR 5 cut(s) 6, 61, 144, 159, 228
FatI CATG 1 cut(s) 142
Fnu4HI GCNGC 1 cut(s) 210
Fsp4HI GCNGC 1 cut(s) 210
GluI GCNGC 1 cut(s) 210
GsuI CTGGAG 1 cut(s) 114
Hin1II CATG 1 cut(s) 146
HphI GGTGA 3 cut(s) 128, 146, 167
Hpy166II GTNNAC 1 cut(s) 175
Hpy188III TCNNGA 1 cut(s) 195
Hpy8I GTNNAC 1 cut(s) 175
HpyCH4III ACNGT 2 cut(s) 103, 138
HpyCH4V TGCA 3 cut(s) 78, 89, 209
HpyF3I CTNAG 1 cut(s) 187
Hsp92II CATG 1 cut(s) 146
LpnPI CCDG 4 cut(s) 78, 105, 180, 192
Lsp1109I GCAGC 1 cut(s) 221
MboII GAAGA 2 cut(s) 5, 53
MluCI AATT 3 cut(s) 119, 168, 214
MseI TTAA 1 cut(s) 167
MslI CAYNNNNRTG 1 cut(s) 180
MspR9I CCNGG 1 cut(s) 93
MvaI CCWGG 1 cut(s) 93
NlaIII CATG 1 cut(s) 146
PflFI GACNNNGTC 1 cut(s) 138
PkrI GCNGC 1 cut(s) 211
Psp6I CCWGG 1 cut(s) 91
PspGI CCWGG 1 cut(s) 91
PsyI GACNNNGTC 1 cut(s) 138
RseI CAYNNNNRTG 1 cut(s) 180
SaqAI TTAA 1 cut(s) 167
SatI GCNGC 1 cut(s) 210
ScrFI CCNGG 1 cut(s) 93
SetI ASST 4 cut(s) 12, 94, 214, 233
SgeI CNNG 8 cut(s) 104, 105, 143, 155, 191, 207, 213, 215
SmiMI CAYNNNNRTG 1 cut(s) 180
Sse9I AATT 3 cut(s) 119, 168, 214
StyD4I CCNGG 1 cut(s) 91
StyI CCWWGG 1 cut(s) 130
TaaI ACNGT 2 cut(s) 103, 138
TasI AATT 3 cut(s) 119, 168, 214
Tru1I TTAA 1 cut(s) 167
Tru9I TTAA 1 cut(s) 167
TscAI CASTG 2 cut(s) 85, 143
TseI GCWGC 1 cut(s) 209
TspDTI ATGAA 1 cut(s) 208
TspRI CASTG 2 cut(s) 85, 143
Tth111I GACNNNGTC 1 cut(s) 138
XapI RAATTY 1 cut(s) 119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.