Rh2BG309700

DNA replication ATP-dependent helicase nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0002244
Physical Location & Seq
Forward (+)
36189259 .. 36191469
2211 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG309700.1

Sequence Viewer

Length: 801 bp
ATGAGTCAAGACGATGGAGGTGATGAGGTGACATGGAGGACGTCTCCGGTCAATGAAAGACTTAAAGCAGTTTCAAAGCACAAGCCTGAGATGATGAGAGTATTGGCAGACACTTCAAGGCTGAAATCTTTAAGCATTAACCAATGCTCGAAAAACAAGACTTCTGCTGTTGAAGCTGGAAAGGTTGAGAAATCCCTGTCGTTGGTGTCCCTGAATAGGGTTGGCGTGAAAAGGTTTAAACCAGATCAGGATGTAGAACAAAATGGAAATGTGGTCGATAGGACTATTTCTGAAATGGCTGGTAGTCAGAGTCCATTCCGGACTCCACATTCTTTATCATACTGTCATGATAAGCTTCCTAATGCTCTTTCATGTGGTGGAGTTTCTGATGAGCCAGGGTTGAGGCCGCATATAAAGACATTGCTTGAACTCTTAGATCAAGTAGAAGATGTTATAGTGGTGACAGCTCCAACAGAACAGGTCACTCGGGCATCTTCCAATTACAATTTCCTTGTATTGGAGGTATCTGAGAATTGTAGACCTGATGATTCCTCTGGTGCTCAGTGCATGTATAAGGTTCTTCGCTTATTGAATGAGCAAAGTGGAGAAGAGTGTGCTGTGTATTTATGGGTAGAGTGGTTTTGCAGTGTCATTGCACCTGGAGATACTGTCAATGTTATTGGTGAATTTGATGACCAAGGACAGTGTCATGTGGATAATCACCATAAGTTTTTAATTGTTCACCCTGATGACTTAGTGTCTGGAACTCGGGTTGCAGCTAATTTCATTTGTCATAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 map03030
Pfam Domains
Protein Families
AAA_11 AAA_12 Cas_Cas4 Dna2

Protein Analysis

266

Amino Acids

29.48

Weight (kDa)

5.48

Isoelectric Point (pI)

38.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dna2 PF08696 199 - 266 7e-18 DNA replication factor Dna2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 44
AccI GTMKAC 1 cut(s) 538
AccIII TCCGGA 1 cut(s) 318
AciI CCGC 1 cut(s) 407
AcsI RAATTY 1 cut(s) 686
AcyI GRCGYC 1 cut(s) 41
AfiI CCNNNNNNNGG 4 cut(s) 202, 216, 217, 517
AgsI TTSAA 5 cut(s) 75, 117, 173, 428, 592
AhdI GACNNNNNGTC 1 cut(s) 757
AjnI CCWGG 2 cut(s) 394, 658
AjuI GAANNNNNNNTTGG 2 cut(s) 491, 523
AluBI AGCT 4 cut(s) 176, 355, 467, 779
AluI AGCT 4 cut(s) 176, 355, 467, 779
Alw21I GWGCWC 1 cut(s) 562
Alw26I GTCTC 1 cut(s) 48
Ama87I CYCGRG 2 cut(s) 486, 768
Aor13HI TCCGGA 1 cut(s) 318
AoxI GGCC 1 cut(s) 404
ApeKI GCWGC 1 cut(s) 776
ApoI RAATTY 1 cut(s) 686
AsuHPI GGTGA 6 cut(s) 32, 40, 472, 695, 713, 734
AvaI CYCGRG 2 cut(s) 486, 768
Bbv12I GWGCWC 1 cut(s) 562
BbvI GCAGC 1 cut(s) 788
BccI CCATC 1 cut(s) 8
BciT130I CCWGG 2 cut(s) 396, 660
BcoDI GTCTC 1 cut(s) 48
BisI GCNGC 2 cut(s) 407, 777
BlsI GCNGC 2 cut(s) 408, 778
Bme1390I CCNGG 2 cut(s) 396, 660
BmeRI GACNNNNNGTC 1 cut(s) 757
BmeT110I CYCGRG 2 cut(s) 486, 768
BmrFI CCNGG 2 cut(s) 396, 660
BmsI GCATC 1 cut(s) 500
BplI GAGNNNNNCTC 2 cut(s) 28, 60
BpmI CTGGAG 1 cut(s) 681
BsaHI GRCGYC 1 cut(s) 41
BsaJI CCNNGG 2 cut(s) 395, 697
BsaWI WCCGGW 2 cut(s) 46, 318
BsaXI ACNNNNNCTCC 2 cut(s) 654, 684
Bsc4I CCNNNNNNNGG 4 cut(s) 202, 216, 217, 517
Bse3DI GCAATG 2 cut(s) 419, 651
BseAI TCCGGA 1 cut(s) 318
BseBI CCWGG 2 cut(s) 396, 660
BseDI CCNNGG 2 cut(s) 395, 697
BseGI GGATG 1 cut(s) 256
BseLI CCNNNNNNNGG 4 cut(s) 202, 216, 217, 517
BseMI GCAATG 2 cut(s) 419, 651
BseMII CTCAG 3 cut(s) 78, 519, 575
BseXI GCAGC 1 cut(s) 788
BshFI GGCC 1 cut(s) 406
BsiHKAI GWGCWC 1 cut(s) 562
BsiHKCI CYCGRG 2 cut(s) 486, 768
BsiSI CCGG 2 cut(s) 47, 319
BslFI GGGAC 1 cut(s) 193
BslI CCNNNNNNNGG 4 cut(s) 202, 216, 217, 517
BsmAI GTCTC 1 cut(s) 48
BsmBI CGTCTC 1 cut(s) 48
BsmFI GGGAC 1 cut(s) 193
BsnI GGCC 1 cut(s) 406
BsoBI CYCGRG 2 cut(s) 486, 768
Bsp1286I GDGCHC 1 cut(s) 562
Bsp13I TCCGGA 1 cut(s) 318
Bsp143I GATC 2 cut(s) 244, 436
BspACI CCGC 1 cut(s) 407
BspANI GGCC 1 cut(s) 406
BspCNI CTCAG 3 cut(s) 79, 520, 574
BspEI TCCGGA 1 cut(s) 318
BspHI TCATGA 1 cut(s) 346
BsrDI GCAATG 2 cut(s) 419, 651
BssECI CCNNGG 2 cut(s) 395, 697
BssMI GATC 2 cut(s) 244, 436
BssNI GRCGYC 1 cut(s) 41
BssT1I CCWWGG 1 cut(s) 697
Bst2UI CCWGG 2 cut(s) 396, 660
Bst4CI ACNGT 3 cut(s) 344, 670, 705
Bst6I CTCTTC 1 cut(s) 603
BstACI GRCGYC 1 cut(s) 41
BstDEI CTNAG 5 cut(s) 87, 433, 528, 561, 754
BstF5I GGATG 1 cut(s) 256
BstKTI GATC 2 cut(s) 247, 439
BstMAI GTCTC 1 cut(s) 48
BstMBI GATC 2 cut(s) 244, 436
BstMWI GCNNNNNNNGC 1 cut(s) 173
BstNI CCWGG 2 cut(s) 396, 660
BstNSI RCATGY 1 cut(s) 571
BstSCI CCNGG 2 cut(s) 394, 658
BstV1I GCAGC 1 cut(s) 788
BsuRI GGCC 1 cut(s) 406
BtsCI GGATG 1 cut(s) 256
BtsI GCAGTG 1 cut(s) 652
BtsIMutI CAGTG 3 cut(s) 569, 652, 710
CciI TCATGA 1 cut(s) 346
CviAII CATG 5 cut(s) 33, 347, 372, 568, 710
CviJI RGCY 9 cut(s) 85, 121, 176, 299, 355, 394, 406, 467, 779
CviKI_1 RGCY 9 cut(s) 85, 121, 176, 299, 355, 394, 406, 467, 779
DdeI CTNAG 5 cut(s) 87, 433, 528, 561, 754
DpnI GATC 2 cut(s) 246, 438
DpnII GATC 2 cut(s) 244, 436
DraI TTTAAA 1 cut(s) 238
DriI GACNNNNNGTC 1 cut(s) 757
Eam1104I CTCTTC 1 cut(s) 603
Eam1105I GACNNNNNGTC 1 cut(s) 757
EarI CTCTTC 1 cut(s) 603
Eco130I CCWWGG 1 cut(s) 697
Eco88I CYCGRG 2 cut(s) 486, 768
EcoRII CCWGG 2 cut(s) 394, 658
EcoT14I CCWWGG 1 cut(s) 697
ErhI CCWWGG 1 cut(s) 697
Esp3I CGTCTC 1 cut(s) 48
FaeI CATG 5 cut(s) 36, 350, 375, 571, 713
FaqI GGGAC 1 cut(s) 193
FatI CATG 5 cut(s) 32, 346, 371, 567, 709
FblI GTMKAC 1 cut(s) 538
Fnu4HI GCNGC 2 cut(s) 407, 777
FokI GGATG 1 cut(s) 263
Fsp4HI GCNGC 2 cut(s) 407, 777
GluI GCNGC 2 cut(s) 407, 777
GsuI CTGGAG 1 cut(s) 681
HaeIII GGCC 1 cut(s) 406
HapII CCGG 2 cut(s) 47, 319
Hin1I GRCGYC 1 cut(s) 41
Hin1II CATG 5 cut(s) 36, 350, 375, 571, 713
HindIII AAGCTT 1 cut(s) 353
HinfI GANTC 4 cut(s) 4, 310, 322, 548
HpaII CCGG 2 cut(s) 47, 319
HphI GGTGA 6 cut(s) 32, 40, 472, 695, 713, 734
Hpy166II GTNNAC 2 cut(s) 539, 742
Hpy188I TCNGA 4 cut(s) 292, 309, 388, 529
Hpy188III TCNNGA 5 cut(s) 8, 248, 319, 347, 762
Hpy8I GTNNAC 2 cut(s) 539, 742
HpyCH4III ACNGT 3 cut(s) 344, 670, 705
HpyCH4IV ACGT 1 cut(s) 41
HpyCH4V TGCA 4 cut(s) 567, 645, 656, 776
HpyF10VI GCNNNNNNNGC 1 cut(s) 173
HpyF3I CTNAG 5 cut(s) 87, 433, 528, 561, 754
HpySE526I ACGT 1 cut(s) 41
Hsp92I GRCGYC 1 cut(s) 41
Hsp92II CATG 5 cut(s) 36, 350, 375, 571, 713
Kpn2I TCCGGA 1 cut(s) 318
Kzo9I GATC 2 cut(s) 244, 436
LmnI GCTCC 1 cut(s) 472
Lsp1109I GCAGC 1 cut(s) 788
LweI GCATC 1 cut(s) 500
MaeII ACGT 1 cut(s) 41
MaeIII GTNAC 3 cut(s) 28, 460, 481
MalI GATC 2 cut(s) 246, 438
MboI GATC 2 cut(s) 244, 436
MboII GAAGA 4 cut(s) 458, 486, 572, 620
MhlI GDGCHC 1 cut(s) 562
MluCI AATT 6 cut(s) 499, 505, 532, 686, 735, 781
MlyI GAGTC 3 cut(s) 13, 316, 319
MmeI TCCRAC 1 cut(s) 494
MnlI CCTC 6 cut(s) 11, 19, 30, 396, 514, 562
MroI TCCGGA 1 cut(s) 318
MseI TTAA 5 cut(s) 63, 131, 138, 237, 734
MslI CAYNNNNRTG 1 cut(s) 747
MspI CCGG 2 cut(s) 47, 319
MspR9I CCNGG 2 cut(s) 396, 660
MssI GTTTAAAC 1 cut(s) 238
MvaI CCWGG 2 cut(s) 396, 660
MwoI GCNNNNNNNGC 1 cut(s) 173
NdeII GATC 2 cut(s) 244, 436
NlaIII CATG 5 cut(s) 36, 350, 375, 571, 713
NmuCI GTSAC 3 cut(s) 28, 460, 481
NspI RCATGY 1 cut(s) 571
PagI TCATGA 1 cut(s) 346
PfeI GAWTC 1 cut(s) 548
PflFI GACNNNGTC 1 cut(s) 705
PkrI GCNGC 2 cut(s) 408, 778
PleI GAGTC 3 cut(s) 12, 316, 318
PmeI GTTTAAAC 1 cut(s) 238
PpsI GAGTC 3 cut(s) 12, 316, 318
Psp6I CCWGG 2 cut(s) 394, 658
PspGI CCWGG 2 cut(s) 394, 658
PsyI GACNNNGTC 1 cut(s) 705
RseI CAYNNNNRTG 1 cut(s) 747
SaqAI TTAA 5 cut(s) 63, 131, 138, 237, 734
SatI GCNGC 2 cut(s) 407, 777
Sau3AI GATC 2 cut(s) 244, 436
SchI GAGTC 3 cut(s) 13, 316, 319
ScrFI CCNGG 2 cut(s) 396, 660
SduI GDGCHC 1 cut(s) 562
SfaNI GCATC 1 cut(s) 500
SmiMI CAYNNNNRTG 1 cut(s) 747
Sse9I AATT 6 cut(s) 499, 505, 532, 686, 735, 781
SsiI CCGC 1 cut(s) 407
StyD4I CCNGG 2 cut(s) 394, 658
StyI CCWWGG 1 cut(s) 697
TaaI ACNGT 3 cut(s) 344, 670, 705
TaiI ACGT 1 cut(s) 44
TaqI TCGA 2 cut(s) 149, 276
TasI AATT 6 cut(s) 499, 505, 532, 686, 735, 781
TauI GCSGC 1 cut(s) 409
TfiI GAWTC 1 cut(s) 548
Tru1I TTAA 5 cut(s) 63, 131, 138, 237, 734
Tru9I TTAA 5 cut(s) 63, 131, 138, 237, 734
TscAI CASTG 3 cut(s) 569, 652, 710
TseFI GTSAC 3 cut(s) 28, 460, 481
TseI GCWGC 1 cut(s) 776
Tsp45I GTSAC 3 cut(s) 28, 460, 481
TspDTI ATGAA 3 cut(s) 69, 360, 775
TspRI CASTG 3 cut(s) 569, 652, 710
Tth111I GACNNNGTC 1 cut(s) 705
XapI RAATTY 1 cut(s) 686
XceI RCATGY 1 cut(s) 571
XmiI GTMKAC 1 cut(s) 538
ZraI GACGTC 1 cut(s) 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.