AT1G15730

COBW domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001734
Physical Location & Seq
Reverse (-)
5407401 .. 5410043
2643 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G15730.1

Sequence Viewer

Length: 1347 bp
ATGGCGACGCTGTTGAAACTCGACATAGCCACGACTTTCCTTGCTTTCATCGTTCCCCGAGCTAATACCTCCTTGAACCATCGGTTCGCTTCTGCTAGGCTCAGTACAGCCACAGTTTCCCTCAGAACGAAGTCGTCATCGTTCTATTCCGCGGCGCTTTACTCTGACAGCCGTCGGAGATTCCACTCCGCCGTTGCCTCTGATTCCTCGCTCGCCGTTGTAGATGATGAAGATATCTTCGATGTTGCCAGTGAGATTTTGCCCGACAATCGAATTCCGGCCACTATAATCACCGGGTTCCTGGGCTCTGGTAAGACAACATTGCTAAACCATATATTGACGGGAGATCATGGGAAGCGCATAGCCGTGATTGAGAACGAGTTTGGTGAAGTCGACATTGATGGATCACTTGTGGCAGCTCAAACTGCGGGAGCGGAGGATATAATGATGCTTAACAATGGCTGTCTGTGTTGTACTGTTAGGGGTGATCTTGTGAGGATGATTTCTGAAATGGTCCAGACCAAGAAAGGAAGGTTCGACCATATCGTTATTGAGACGACAGGATTGGCTAACCCTGCTCCGATTATTCAAACTTTCTATGCTGAGGATGAAATTTTCAATGATGTCAAGCTGGATGGGGTTGTCACTCTGGTTGATGCTAAACATGCTCGTTTGCATCTAGATGAGGTCAAACCTGAAGGCTATGTCAATGAGGCGGTTGAACAAATAGCTTACGCGGATCGTATCATTGTTAACAAGACTGATCTTGTTGGTGAGCCAGAACTAGCTTCAGTGATGCAGCGGATAAAGACCATAAACAGCATGGCTCACATGAAGCGGACAAAGTATGGGAAGGTTGACTTGGATTATGTTCTTGGAATTGGAGGGTTTGATCTAGAAAGAATTGAAAGCTCTGTGAATGAAGAAGAGAAAGAAGATCGCGAGGGTCATGATGATCATCACCATGGTCATGACTGCCATGATCACCACAATGAGCATGAGCATGAGCATGAACACGAGCATCACCATTCTCATGATCACACCCATGACCCTGGTGTTGGTTCAGTCAGTATAGTTTGCGAAGGAGACTTAGACCTCGAGAAGGCTAACATGTGGCTTGGGGCGCTATTGTACCAACGTAGTGAGGATATCTACAGAATGAAAGGTATCCTCTCCGTACAAGACATGGACGAGAGATTCGTGTTTCAGGGAGTTCATGAAATATTTGAAGGATCACCTGACCGGTTATGGAGAAAAGATGAAACAAGAACCAACAAGATTGTTTTCATCGGCAAGAATTTGAACCGGGAGGAGTTAGAGATGGGTTTCAGAGCTTGCTTGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CobW_C cobW

Protein Analysis

448

Amino Acids

50.26

Weight (kDa)

5.26

Isoelectric Point (pI)

35.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cobW PF02492 93 - 278 2.5e-59 CobW/HypB/UreG, nucleotide-binding domain
CobW_C PF07683 354 - 447 6.8e-24 Cobalamin synthesis protein cobW C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 434
AccI GTMKAC 1 cut(s) 393
AccII CGCG 3 cut(s) 152, 737, 942
AciI CCGC 9 cut(s) 150, 152, 189, 428, 434, 716, 737, 802, 838
AclWI GGATC 3 cut(s) 412, 747, 1240
AcoI YGGCCR 1 cut(s) 279
AcsI RAATTY 3 cut(s) 273, 612, 1297
AcuI CTGAAG 2 cut(s) 717, 774
AfaI GTAC 4 cut(s) 106, 475, 1133, 1179
AfiI CCNNNNNNNGG 2 cut(s) 1058, 1102
AflIII ACRYGT 1 cut(s) 1110
AgeI ACCGGT 1 cut(s) 1242
AgsI TTSAA 8 cut(s) 16, 76, 590, 619, 722, 908, 1229, 1303
AjnI CCWGG 2 cut(s) 300, 1051
AjuI GAANNNNNNNTTGG 2 cut(s) 845, 877
AluBI AGCT 7 cut(s) 62, 419, 631, 731, 788, 912, 1334
AluI AGCT 7 cut(s) 62, 419, 631, 731, 788, 912, 1334
Alw26I GTCTC 2 cut(s) 548, 1080
AlwI GGATC 3 cut(s) 412, 747, 1240
Ama87I CYCGRG 2 cut(s) 57, 1097
AoxI GGCC 1 cut(s) 279
ApeKI GCWGC 2 cut(s) 416, 799
ApoI RAATTY 3 cut(s) 273, 612, 1297
ArsI GACNNNNNNTTYG 2 cut(s) 1181, 1213
AsiGI ACCGGT 1 cut(s) 1242
AspLEI GCGC 3 cut(s) 157, 360, 1126
AspS9I GGNCC 1 cut(s) 514
AsuC2I CCSGG 2 cut(s) 295, 1307
AsuHPI GGTGA 8 cut(s) 283, 398, 497, 785, 953, 977, 1016, 1227
AvaI CYCGRG 2 cut(s) 57, 1097
AvaII GGWCC 1 cut(s) 514
BanII GRGCYC 1 cut(s) 308
BauI CACGAG 1 cut(s) 1016
BbvCI CCTCAGC 1 cut(s) 603
BbvI GCAGC 2 cut(s) 428, 811
BccI CCATC 4 cut(s) 87, 395, 629, 1315
BceAI ACGGC 4 cut(s) 156, 176, 200, 350
BciT130I CCWGG 2 cut(s) 302, 1053
BciVI GTATCC 1 cut(s) 1178
BclI TGATCA 3 cut(s) 955, 982, 1036
BcnI CCSGG 2 cut(s) 295, 1307
BcoDI GTCTC 2 cut(s) 548, 1080
BfaI CTAG 4 cut(s) 96, 680, 785, 896
BfmI CTRYAG 1 cut(s) 1153
BfoI RGCGCY 2 cut(s) 158, 1127
BfuI GTATCC 1 cut(s) 1178
BisI GCNGC 3 cut(s) 153, 417, 800
BlsI GCNGC 3 cut(s) 154, 418, 801
Bme1390I CCNGG 4 cut(s) 295, 302, 1053, 1307
Bme18I GGWCC 1 cut(s) 514
BmeT110I CYCGRG 2 cut(s) 57, 1097
BmgT120I GGNCC 1 cut(s) 514
BmiI GGNNCC 1 cut(s) 299
BmrFI CCNGG 4 cut(s) 295, 302, 1053, 1307
BmsI GCATC 5 cut(s) 438, 646, 685, 786, 1030
BoxI GACNNNNGTC 1 cut(s) 171
Bpu10I CCTNAGC 1 cut(s) 603
BpuMI CCSGG 2 cut(s) 295, 1307
BsaBI GATNNNNATC 1 cut(s) 957
BsaJI CCNNGG 4 cut(s) 150, 301, 964, 1051
BsaWI WCCGGW 1 cut(s) 1242
Bsc4I CCNNNNNNNGG 2 cut(s) 1058, 1102
Bse118I RCCGGY 1 cut(s) 1242
Bse1I ACTGG 1 cut(s) 249
Bse3DI GCAATG 1 cut(s) 320
Bse8I GATNNNNATC 1 cut(s) 957
BseBI CCWGG 2 cut(s) 302, 1053
BseDI CCNNGG 4 cut(s) 150, 301, 964, 1051
BseGI GGATG 3 cut(s) 504, 613, 640
BseJI GATNNNNATC 1 cut(s) 957
BseLI CCNNNNNNNGG 2 cut(s) 1058, 1102
BseMI GCAATG 1 cut(s) 320
BseMII CTCAG 3 cut(s) 115, 136, 594
BseNI ACTGG 1 cut(s) 249
BseRI GAGGAG 1 cut(s) 1325
BseXI GCAGC 2 cut(s) 428, 811
Bsh1236I CGCG 3 cut(s) 152, 737, 942
BshFI GGCC 1 cut(s) 281
BshTI ACCGGT 1 cut(s) 1242
BsiHKCI CYCGRG 2 cut(s) 57, 1097
BsiSI CCGG 4 cut(s) 278, 294, 1243, 1306
BslI CCNNNNNNNGG 2 cut(s) 1058, 1102
BsmAI GTCTC 2 cut(s) 548, 1080
BsmBI CGTCTC 1 cut(s) 548
BsnI GGCC 1 cut(s) 281
BsoBI CYCGRG 2 cut(s) 57, 1097
Bsp1286I GDGCHC 1 cut(s) 308
Bsp19I CCATGG 1 cut(s) 964
Bsp68I TCGCGA 1 cut(s) 942
BspACI CCGC 9 cut(s) 150, 152, 189, 428, 434, 716, 737, 802, 838
BspANI GGCC 1 cut(s) 281
BspCNI CTCAG 3 cut(s) 114, 135, 595
BspFNI CGCG 3 cut(s) 152, 737, 942
BspHI TCATGA 4 cut(s) 949, 970, 1033, 1216
BspLI GGNNCC 1 cut(s) 299
BspPI GGATC 3 cut(s) 412, 747, 1240
BsrBI CCGCTC 1 cut(s) 434
BsrDI GCAATG 1 cut(s) 320
BsrFI RCCGGY 1 cut(s) 1242
BsrI ACTGG 1 cut(s) 249
BssAI RCCGGY 1 cut(s) 1242
BssECI CCNNGG 4 cut(s) 150, 301, 964, 1051
BssSI CACGAG 1 cut(s) 1016
BssT1I CCWWGG 1 cut(s) 964
Bst2BI CACGAG 1 cut(s) 1016
Bst2UI CCWGG 2 cut(s) 302, 1053
Bst4CI ACNGT 2 cut(s) 115, 478
Bst6I CTCTTC 1 cut(s) 921
BstC8I GCNNGC 2 cut(s) 213, 1336
BstDEI CTNAG 4 cut(s) 101, 122, 603, 1090
BstDSI CCRYGG 2 cut(s) 150, 964
BstENI CCTNNNNNAGG 1 cut(s) 1100
BstF5I GGATG 3 cut(s) 504, 613, 640
BstFNI CGCG 3 cut(s) 152, 737, 942
BstH2I RGCGCY 2 cut(s) 158, 1127
BstHHI GCGC 3 cut(s) 157, 360, 1126
BstMAI GTCTC 2 cut(s) 548, 1080
BstMWI GCNNNNNNNGC 4 cut(s) 425, 575, 665, 1123
BstNI CCWGG 2 cut(s) 302, 1053
BstNSI RCATGY 2 cut(s) 668, 1114
BstPAI GACNNNNGTC 1 cut(s) 171
BstSCI CCNGG 4 cut(s) 293, 300, 1051, 1305
BstSFI CTRYAG 1 cut(s) 1153
BstUI CGCG 3 cut(s) 152, 737, 942
BstV1I GCAGC 2 cut(s) 428, 811
BstXI CCANNNNNNTGG 1 cut(s) 1052
BsuI GTATCC 1 cut(s) 1178
BsuRI GGCC 1 cut(s) 281
BtgI CCRYGG 2 cut(s) 150, 964
BtsCI GGATG 3 cut(s) 504, 613, 640
BtsIMutI CAGTG 2 cut(s) 256, 798
BtuMI TCGCGA 1 cut(s) 942
Cac8I GCNNGC 2 cut(s) 213, 1336
CciI TCATGA 4 cut(s) 949, 970, 1033, 1216
CfoI GCGC 3 cut(s) 157, 360, 1126
Cfr10I RCCGGY 1 cut(s) 1242
Cfr13I GGNCC 1 cut(s) 514
Cfr42I CCGCGG 1 cut(s) 153
CseI GACGC 1 cut(s) 16
Csp6I GTAC 4 cut(s) 105, 474, 1132, 1178
CspAI ACCGGT 1 cut(s) 1242
CviQI GTAC 4 cut(s) 105, 474, 1132, 1178
DdeI CTNAG 4 cut(s) 101, 122, 603, 1090
EaeI YGGCCR 1 cut(s) 279
Eam1104I CTCTTC 1 cut(s) 921
EarI CTCTTC 1 cut(s) 921
EciI GGCGGA 1 cut(s) 178
Eco130I CCWWGG 1 cut(s) 964
Eco24I GRGCYC 1 cut(s) 308
Eco32I GATATC 2 cut(s) 235, 1150
Eco47I GGWCC 1 cut(s) 514
Eco57I CTGAAG 2 cut(s) 717, 774
Eco88I CYCGRG 2 cut(s) 57, 1097
EcoNI CCTNNNNNAGG 1 cut(s) 1100
EcoRI GAATTC 1 cut(s) 273
EcoRII CCWGG 2 cut(s) 300, 1051
EcoRV GATATC 2 cut(s) 235, 1150
EcoT14I CCWWGG 1 cut(s) 964
EcoT38I GRGCYC 1 cut(s) 308
ErhI CCWWGG 1 cut(s) 964
Esp3I CGTCTC 1 cut(s) 548
FalI AAGNNNNNCTT 2 cut(s) 845, 877
FauI CCCGC 1 cut(s) 421
FbaI TGATCA 3 cut(s) 955, 982, 1036
FblI GTMKAC 1 cut(s) 393
Fnu4HI GCNGC 3 cut(s) 153, 417, 800
FokI GGATG 3 cut(s) 511, 620, 647
FriOI GRGCYC 1 cut(s) 308
Fsp4HI GCNGC 3 cut(s) 153, 417, 800
FspBI CTAG 4 cut(s) 96, 680, 785, 896
GlaI GCGC 3 cut(s) 156, 359, 1125
GluI GCNGC 3 cut(s) 153, 417, 800
HaeII RGCGCY 2 cut(s) 158, 1127
HaeIII GGCC 1 cut(s) 281
HapII CCGG 4 cut(s) 278, 294, 1243, 1306
HgaI GACGC 1 cut(s) 16
HhaI GCGC 3 cut(s) 157, 360, 1126
Hin6I GCGC 3 cut(s) 155, 358, 1124
HinP1I GCGC 3 cut(s) 155, 358, 1124
HincII GTYRAC 3 cut(s) 394, 754, 859
HindII GTYRAC 3 cut(s) 394, 754, 859
HinfI GANTC 3 cut(s) 180, 203, 1197
HpaI GTTAAC 1 cut(s) 754
HpaII CCGG 4 cut(s) 278, 294, 1243, 1306
HphI GGTGA 8 cut(s) 283, 398, 497, 785, 953, 977, 1016, 1227
Hpy166II GTNNAC 3 cut(s) 394, 754, 859
Hpy188I TCNGA 7 cut(s) 125, 166, 177, 202, 508, 582, 1331
Hpy188III TCNNGA 9 cut(s) 517, 680, 896, 941, 950, 971, 1034, 1099, 1217
Hpy8I GTNNAC 3 cut(s) 394, 754, 859
Hpy99I CGWCG 2 cut(s) 10, 177
HpyAV CCTTC 6 cut(s) 525, 692, 847, 1076, 1096, 1223
HpyCH4III ACNGT 2 cut(s) 115, 478
HpyCH4IV ACGT 1 cut(s) 1138
HpyCH4V TGCA 2 cut(s) 676, 799
HpyF10VI GCNNNNNNNGC 4 cut(s) 425, 575, 665, 1123
HpyF3I CTNAG 4 cut(s) 101, 122, 603, 1090
HpySE526I ACGT 1 cut(s) 1138
HspAI GCGC 3 cut(s) 155, 358, 1124
Ksp22I TGATCA 3 cut(s) 955, 982, 1036
KspAI GTTAAC 1 cut(s) 754
KspI CCGCGG 1 cut(s) 153
LmnI GCTCC 2 cut(s) 431, 583
Lsp1109I GCAGC 2 cut(s) 428, 811
LweI GCATC 5 cut(s) 438, 646, 685, 786, 1030
MaeI CTAG 4 cut(s) 96, 680, 785, 896
MaeII ACGT 1 cut(s) 1138
MaeIII GTNAC 1 cut(s) 643
MbiI CCGCTC 1 cut(s) 434
MboII GAAGA 5 cut(s) 229, 242, 935, 938, 947
MhlI GDGCHC 1 cut(s) 308
MluCI AATT 5 cut(s) 273, 612, 879, 903, 1297
MmeI TCCRAC 1 cut(s) 155
MseI TTAA 2 cut(s) 453, 753
MslI CAYNNNNRTG 9 cut(s) 365, 681, 963, 969, 990, 1002, 1008, 1032, 1044
MspA1I CMGCKG 2 cut(s) 152, 802
MspI CCGG 4 cut(s) 278, 294, 1243, 1306
MspR9I CCNGG 4 cut(s) 295, 302, 1053, 1307
MvaI CCWGG 2 cut(s) 302, 1053
MvnI CGCG 3 cut(s) 152, 737, 942
MwoI GCNNNNNNNGC 4 cut(s) 425, 575, 665, 1123
NciI CCSGG 2 cut(s) 295, 1307
NcoI CCATGG 1 cut(s) 964
NlaIV GGNNCC 1 cut(s) 299
NmuCI GTSAC 1 cut(s) 643
NruI TCGCGA 1 cut(s) 942
NspI RCATGY 2 cut(s) 668, 1114
PaeR7I CTCGAG 1 cut(s) 1097
PagI TCATGA 4 cut(s) 949, 970, 1033, 1216
PciI ACATGT 1 cut(s) 1110
PcsI WCGNNNNNNNCGW 2 cut(s) 543, 1197
PfeI GAWTC 3 cut(s) 180, 203, 1197
PinAI ACCGGT 1 cut(s) 1242
PkrI GCNGC 3 cut(s) 154, 418, 801
PscI ACATGT 1 cut(s) 1110
PshAI GACNNNNGTC 1 cut(s) 171
Psp6I CCWGG 2 cut(s) 300, 1051
PspGI CCWGG 2 cut(s) 300, 1051
PspN4I GGNNCC 1 cut(s) 299
PspPI GGNCC 1 cut(s) 514
RruI TCGCGA 1 cut(s) 942
RsaI GTAC 4 cut(s) 106, 475, 1133, 1179
RsaNI GTAC 4 cut(s) 105, 474, 1132, 1178
RseI CAYNNNNRTG 9 cut(s) 365, 681, 963, 969, 990, 1002, 1008, 1032, 1044
SacII CCGCGG 1 cut(s) 153
SalI GTCGAC 1 cut(s) 392
SaqAI TTAA 2 cut(s) 453, 753
SatI GCNGC 3 cut(s) 153, 417, 800
Sau96I GGNCC 1 cut(s) 514
ScrFI CCNGG 4 cut(s) 295, 302, 1053, 1307
SduI GDGCHC 1 cut(s) 308
SfaNI GCATC 5 cut(s) 438, 646, 685, 786, 1030
SfcI CTRYAG 1 cut(s) 1153
Sfr274I CTCGAG 1 cut(s) 1097
Sfr303I CCGCGG 1 cut(s) 153
SgrBI CCGCGG 1 cut(s) 153
SinI GGWCC 1 cut(s) 514
SlaI CTCGAG 1 cut(s) 1097
SmiMI CAYNNNNRTG 9 cut(s) 365, 681, 963, 969, 990, 1002, 1008, 1032, 1044
SmlI CTYRAG 1 cut(s) 1097
SmoI CTYRAG 1 cut(s) 1097
Sse9I AATT 5 cut(s) 273, 612, 879, 903, 1297
SsiI CCGC 9 cut(s) 150, 152, 189, 428, 434, 716, 737, 802, 838
SspI AATATT 1 cut(s) 1224
SspMI CTAG 4 cut(s) 96, 680, 785, 896
StyD4I CCNGG 4 cut(s) 293, 300, 1051, 1305
StyI CCWWGG 1 cut(s) 964
TaaI ACNGT 2 cut(s) 115, 478
TaiI ACGT 1 cut(s) 1141
TaqI TCGA 6 cut(s) 21, 240, 271, 393, 537, 1098
TasI AATT 5 cut(s) 273, 612, 879, 903, 1297
TatI WGTACW 2 cut(s) 104, 473
TauI GCSGC 1 cut(s) 155
TfiI GAWTC 3 cut(s) 180, 203, 1197
Tru1I TTAA 2 cut(s) 453, 753
Tru9I TTAA 2 cut(s) 453, 753
TscAI CASTG 2 cut(s) 256, 798
TseFI GTSAC 1 cut(s) 643
TseI GCWGC 2 cut(s) 416, 799
Tsp45I GTSAC 1 cut(s) 643
TspGWI ACGGA 1 cut(s) 1165
TspRI CASTG 2 cut(s) 256, 798
VpaK11BI GGWCC 1 cut(s) 514
XagI CCTNNNNNAGG 1 cut(s) 1100
XapI RAATTY 3 cut(s) 273, 612, 1297
XbaI TCTAGA 2 cut(s) 679, 895
XceI RCATGY 2 cut(s) 668, 1114
XcmI CCANNNNNNNNNTGG 1 cut(s) 820
XhoI CTCGAG 1 cut(s) 1097
XmiI GTMKAC 1 cut(s) 393
XspI CTAG 4 cut(s) 96, 680, 785, 896
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.