FvH4_5g23620

COBW domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001734
Physical Location & Seq
Forward (+)
14963268 .. 14968310
5043 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g23620.t1

Sequence Viewer

Length: 1215 bp
ATGGCTTCGAGAATCCTACTCTCAAGAACATCCCTCAACAATTTCAATCCTACCCTCAGAACCCTCTGCTCCTTCGTTTCCCGGGAATTCAATTCCGGCAATCGTCAGCCTGGGTTTTCCAGGAGCATGGTCTCTGCTTCATCAACCTACAATCTCGACCAGAATGCCACCTTGGGTCTCGATACCCGTGTCCCGGCCACCATTATCACCGGCTTTCTCGGTTCCGGAAAGACTACATTGCTCAATCATATACTAACCTCTCAACATGGGAAACGGATTGCGGTCATCGAAAATGAGTTTGGTGAGGTGGATATTGATGGCTCATTGGTTGCTAGTCATTTGTCGTCGAATGAGGACATTGTCATGGTTAATAATGGTTGCCTGTGTTGTACTGTTCGGGGTGATCTAGTCAAAATGATCTTGCAATTGGTGAAGGACAAGCGAGACAAGTTTGATCATATTGTGATAGAAACCACAGGCCTCGCTAAGCCATCTCCTGTTATAGAAACGTTTTGTATGGATGAGCTAGTTTCACGACATGTGAAACTGGATGGAGTTGTTACTTTGGTGGATTGCAAGCACGCAATGCAACATCTGAATGAAGTTAAACCGCGATTTGTGGTGAATGAGGCAGTAGAACAAGTTGCATACGCTGATCGTATTATATTGAACAAGATAGACTTGGTGACCGAGACGGAGTTAGAGTCAGTGACCAAAAAAATCAAGCATATTAATGGGATGGCGCAAATCAAGCTAGCTAAATTTGGATCTGTGGACATGGACTTTGTTCTGGATATCGGAGGATATGATCTTGAAAGGATTAATAATGAAGTTCAAGTTGATAGTTCCTGTTCGTCCTCACATCACCATGAAGCTGGACATGACCATGACAACCATCATCATGACCATGTACATGATTCCACTGTCTCAAGTGTCAGTATTGTTGCCGAGGGAACCCTTGATCTTGATGAGGTAGATGACTGGTTGGAAAGACTGATTGAAGAAAAGGGGGAGGACTTGTACAGGATGAAGGGTGTTTTATGTGTTAACGGTTCCGATCAACGTTATGTCTTCCAGGGAGTGCACTCTACGTTGGATGGCTGCCCAGGAGGCACATGGGGACCCGAGGAGAAGAAGATTAACAAGCTTGTGTTCATAGGAAGGAATCTGGATGAAACTAGTCTGAGAAAAGGTTTCAGAGGTTGTCTAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CobW_C cobW

Protein Analysis

405

Amino Acids

44.78

Weight (kDa)

5.66

Isoelectric Point (pI)

35.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cobW PF02492 65 - 250 1.9e-58 CobW/HypB/UreG, nucleotide-binding domain
CobW_C PF07683 309 - 403 2.7e-23 Cobalamin synthesis protein cobW C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 613
AccIII TCCGGA 1 cut(s) 224
AciI CCGC 2 cut(s) 281, 611
AclI AACGTT 2 cut(s) 509, 1063
AclWI GGATC 1 cut(s) 775
AcoI YGGCCR 1 cut(s) 195
AcsI RAATTY 2 cut(s) 86, 761
AfaI GTAC 3 cut(s) 391, 912, 1022
AfiI CCNNNNNNNGG 1 cut(s) 193
AflIII ACRYGT 1 cut(s) 538
AgsI TTSAA 6 cut(s) 46, 91, 670, 815, 836, 1001
AhlI ACTAGT 1 cut(s) 1178
AjnI CCWGG 4 cut(s) 109, 119, 1074, 1105
AjuI GAANNNNNNNTTGG 4 cut(s) 155, 187, 282, 314
AluBI AGCT 5 cut(s) 526, 754, 758, 875, 1147
AluI AGCT 5 cut(s) 526, 754, 758, 875, 1147
Alw21I GWGCWC 1 cut(s) 1086
Alw26I GTCTC 5 cut(s) 136, 182, 438, 686, 931
Alw44I GTGCAC 1 cut(s) 1082
AlwI GGATC 1 cut(s) 775
Ama87I CYCGRG 2 cut(s) 81, 1124
Aor13HI TCCGGA 1 cut(s) 224
AoxI GGCC 2 cut(s) 195, 478
ApaLI GTGCAC 1 cut(s) 1082
ApeKI GCWGC 1 cut(s) 1101
ApoI RAATTY 2 cut(s) 86, 761
ArsI GACNNNNNNTTYG 2 cut(s) 767, 799
AseI ATTAAT 2 cut(s) 732, 822
AspLEI GCGC 1 cut(s) 745
AspS9I GGNCC 1 cut(s) 1121
AsuC2I CCSGG 3 cut(s) 82, 83, 194
AsuHPI GGTGA 7 cut(s) 199, 314, 413, 442, 634, 697, 857
AsuNHI GCTAGC 1 cut(s) 754
AvaI CYCGRG 2 cut(s) 81, 1124
AvaII GGWCC 1 cut(s) 1121
BaeGI GKGCMC 1 cut(s) 1086
BaeI ACNNNNGTAYC 2 cut(s) 174, 207
BbsI GAAGAC 1 cut(s) 1063
Bbv12I GWGCWC 1 cut(s) 1086
BbvI GCAGC 1 cut(s) 1088
BccI CCATC 6 cut(s) 311, 499, 545, 733, 903, 1091
BcgI CGANNNNNNTGC 2 cut(s) 146, 180
BciT130I CCWGG 4 cut(s) 111, 121, 1076, 1107
BclI TGATCA 1 cut(s) 454
BcnI CCSGG 3 cut(s) 82, 83, 194
BcoDI GTCTC 5 cut(s) 136, 182, 438, 686, 931
BcuI ACTAGT 1 cut(s) 1178
BfaI CTAG 6 cut(s) 333, 407, 527, 755, 1179, 1208
BglI GCCNNNNNGGC 1 cut(s) 1110
BisI GCNGC 1 cut(s) 1102
BlpI GCTNAGC 1 cut(s) 486
BlsI GCNGC 1 cut(s) 1103
Bme1390I CCNGG 7 cut(s) 82, 83, 111, 121, 194, 1076, 1107
Bme18I GGWCC 1 cut(s) 1121
BmeT110I CYCGRG 2 cut(s) 81, 1124
BmgT120I GGNCC 1 cut(s) 1121
BmiI GGNNCC 5 cut(s) 223, 955, 1054, 1122, 1123
BmrFI CCNGG 7 cut(s) 82, 83, 111, 121, 194, 1076, 1107
BmtI GCTAGC 1 cut(s) 758
BpiI GAAGAC 1 cut(s) 1063
Bpu1102I GCTNAGC 1 cut(s) 486
BpuEI CTTGAG 2 cut(s) 7, 913
BpuMI CCSGG 3 cut(s) 82, 83, 194
BsaI GGTCTC 2 cut(s) 136, 182
BsaJI CCNNGG 7 cut(s) 81, 110, 171, 948, 1075, 1105, 1125
BsaWI WCCGGW 1 cut(s) 224
BsaXI ACNNNNNCTCC 4 cut(s) 689, 719, 1004, 1034
Bsc4I CCNNNNNNNGG 1 cut(s) 193
Bse118I RCCGGY 1 cut(s) 209
Bse1I ACTGG 2 cut(s) 552, 986
Bse3DI GCAATG 2 cut(s) 236, 591
BseAI TCCGGA 1 cut(s) 224
BseBI CCWGG 4 cut(s) 111, 121, 1076, 1107
BseDI CCNNGG 7 cut(s) 81, 110, 171, 948, 1075, 1105, 1125
BseGI GGATG 7 cut(s) 29, 526, 556, 744, 1032, 1102, 1177
BseLI CCNNNNNNNGG 1 cut(s) 193
BseMI GCAATG 2 cut(s) 236, 591
BseMII CTCAG 2 cut(s) 70, 1175
BseNI ACTGG 2 cut(s) 552, 986
BseRI GAGGAG 1 cut(s) 1142
BseSI GKGCMC 1 cut(s) 1086
BseXI GCAGC 1 cut(s) 1088
Bsh1236I CGCG 1 cut(s) 613
BshFI GGCC 2 cut(s) 197, 480
BsiHKAI GWGCWC 1 cut(s) 1086
BsiHKCI CYCGRG 2 cut(s) 81, 1124
BsiSI CCGG 5 cut(s) 82, 96, 194, 210, 225
BslFI GGGAC 2 cut(s) 176, 1134
BslI CCNNNNNNNGG 1 cut(s) 193
BsmAI GTCTC 5 cut(s) 136, 182, 438, 686, 931
BsmBI CGTCTC 1 cut(s) 686
BsmFI GGGAC 2 cut(s) 176, 1134
BsmI GAATGC 1 cut(s) 169
BsnI GGCC 2 cut(s) 197, 480
Bso31I GGTCTC 2 cut(s) 136, 182
BsoBI CYCGRG 2 cut(s) 81, 1124
Bsp1286I GDGCHC 1 cut(s) 1086
Bsp13I TCCGGA 1 cut(s) 224
Bsp1407I TGTACA 2 cut(s) 910, 1020
Bsp143I GATC 8 cut(s) 403, 417, 454, 655, 767, 808, 961, 1057
Bsp1720I GCTNAGC 1 cut(s) 486
BspACI CCGC 2 cut(s) 281, 611
BspANI GGCC 2 cut(s) 197, 480
BspCNI CTCAG 2 cut(s) 69, 1176
BspEI TCCGGA 1 cut(s) 224
BspFNI CGCG 1 cut(s) 613
BspHI TCATGA 1 cut(s) 901
BspLI GGNNCC 5 cut(s) 223, 955, 1054, 1122, 1123
BspOI GCTAGC 1 cut(s) 758
BspPI GGATC 1 cut(s) 775
BspTNI GGTCTC 2 cut(s) 136, 182
BsrDI GCAATG 2 cut(s) 236, 591
BsrFI RCCGGY 1 cut(s) 209
BsrGI TGTACA 2 cut(s) 910, 1020
BsrI ACTGG 2 cut(s) 552, 986
BssAI RCCGGY 1 cut(s) 209
BssECI CCNNGG 7 cut(s) 81, 110, 171, 948, 1075, 1105, 1125
BssMI GATC 8 cut(s) 403, 417, 454, 655, 767, 808, 961, 1057
BssT1I CCWWGG 1 cut(s) 171
Bst2UI CCWGG 4 cut(s) 111, 121, 1076, 1107
Bst4CI ACNGT 3 cut(s) 394, 925, 1052
BstAPI GCANNNNNTGC 1 cut(s) 586
BstAUI TGTACA 2 cut(s) 910, 1020
BstC8I GCNNGC 3 cut(s) 578, 582, 756
BstDEI CTNAG 3 cut(s) 56, 486, 1184
BstEII GGTNACC 1 cut(s) 685
BstF5I GGATG 7 cut(s) 29, 526, 556, 744, 1032, 1102, 1177
BstFNI CGCG 1 cut(s) 613
BstHHI GCGC 1 cut(s) 745
BstKTI GATC 8 cut(s) 406, 420, 457, 658, 770, 811, 964, 1060
BstMAI GTCTC 5 cut(s) 136, 182, 438, 686, 931
BstMBI GATC 8 cut(s) 403, 417, 454, 655, 767, 808, 961, 1057
BstMWI GCNNNNNNNGC 3 cut(s) 586, 751, 1110
BstNI CCWGG 4 cut(s) 111, 121, 1076, 1107
BstNSI RCATGY 1 cut(s) 542
BstPI GGTNACC 1 cut(s) 685
BstSCI CCNGG 7 cut(s) 80, 81, 109, 119, 192, 1074, 1105
BstSLI GKGCMC 1 cut(s) 1086
BstUI CGCG 1 cut(s) 613
BstV1I GCAGC 1 cut(s) 1088
BstV2I GAAGAC 1 cut(s) 1063
BstX2I RGATCY 1 cut(s) 767
BstXI CCANNNNNNTGG 2 cut(s) 127, 875
BstYI RGATCY 1 cut(s) 767
BsuRI GGCC 2 cut(s) 197, 480
BtsCI GGATG 7 cut(s) 29, 526, 556, 744, 1032, 1102, 1177
BtsIMutI CAGTG 2 cut(s) 714, 921
Cac8I GCNNGC 3 cut(s) 578, 582, 756
CciI TCATGA 1 cut(s) 901
CfoI GCGC 1 cut(s) 745
Cfr10I RCCGGY 1 cut(s) 209
Cfr13I GGNCC 1 cut(s) 1121
Cfr9I CCCGGG 1 cut(s) 81
Csp6I GTAC 3 cut(s) 390, 911, 1021
CviQI GTAC 3 cut(s) 390, 911, 1021
DdeI CTNAG 3 cut(s) 56, 486, 1184
DpnI GATC 8 cut(s) 405, 419, 456, 657, 769, 810, 963, 1059
DpnII GATC 8 cut(s) 403, 417, 454, 655, 767, 808, 961, 1057
EaeI YGGCCR 1 cut(s) 195
Eco130I CCWWGG 1 cut(s) 171
Eco147I AGGCCT 1 cut(s) 480
Eco31I GGTCTC 2 cut(s) 136, 182
Eco32I GATATC 1 cut(s) 796
Eco47I GGWCC 1 cut(s) 1121
Eco88I CYCGRG 2 cut(s) 81, 1124
Eco91I GGTNACC 1 cut(s) 685
EcoO109I RGGNCCY 1 cut(s) 1121
EcoO65I GGTNACC 1 cut(s) 685
EcoRI GAATTC 1 cut(s) 86
EcoRII CCWGG 4 cut(s) 109, 119, 1074, 1105
EcoRV GATATC 1 cut(s) 796
EcoT14I CCWWGG 1 cut(s) 171
ErhI CCWWGG 1 cut(s) 171
Esp3I CGTCTC 1 cut(s) 686
FalI AAGNNNNNCTT 2 cut(s) 665, 697
FaqI GGGAC 2 cut(s) 176, 1134
FbaI TGATCA 1 cut(s) 454
Fnu4HI GCNGC 1 cut(s) 1102
FokI GGATG 7 cut(s) 16, 533, 563, 751, 1039, 1109, 1184
Fsp4HI GCNGC 1 cut(s) 1102
FspBI CTAG 6 cut(s) 333, 407, 527, 755, 1179, 1208
GlaI GCGC 1 cut(s) 744
GluI GCNGC 1 cut(s) 1102
HaeIII GGCC 2 cut(s) 197, 480
HapII CCGG 5 cut(s) 82, 96, 194, 210, 225
HhaI GCGC 1 cut(s) 745
Hin6I GCGC 1 cut(s) 743
HinP1I GCGC 1 cut(s) 743
HincII GTYRAC 1 cut(s) 1048
HindII GTYRAC 1 cut(s) 1048
HindIII AAGCTT 1 cut(s) 1145
HinfI GANTC 4 cut(s) 12, 704, 917, 1165
HpaI GTTAAC 1 cut(s) 1048
HpaII CCGG 5 cut(s) 82, 96, 194, 210, 225
HphI GGTGA 7 cut(s) 199, 314, 413, 442, 634, 697, 857
Hpy166II GTNNAC 3 cut(s) 775, 1048, 1084
Hpy188I TCNGA 6 cut(s) 59, 597, 800, 1057, 1185, 1199
Hpy8I GTNNAC 3 cut(s) 775, 1048, 1084
Hpy99I CGWCG 1 cut(s) 349
HpyAV CCTTC 4 cut(s) 82, 427, 1024, 1155
HpyCH4III ACNGT 3 cut(s) 394, 925, 1052
HpyCH4IV ACGT 3 cut(s) 509, 1063, 1091
HpyCH4V TGCA 5 cut(s) 424, 576, 589, 647, 1084
HpyF10VI GCNNNNNNNGC 3 cut(s) 586, 751, 1110
HpyF3I CTNAG 3 cut(s) 56, 486, 1184
HpySE526I ACGT 3 cut(s) 509, 1063, 1091
HspAI GCGC 1 cut(s) 743
KflI GGGWCCC 1 cut(s) 1121
Kpn2I TCCGGA 1 cut(s) 224
Ksp22I TGATCA 1 cut(s) 454
KspAI GTTAAC 1 cut(s) 1048
Kzo9I GATC 8 cut(s) 403, 417, 454, 655, 767, 808, 961, 1057
LmnI GCTCC 2 cut(s) 74, 123
Lsp1109I GCAGC 1 cut(s) 1088
MaeI CTAG 6 cut(s) 333, 407, 527, 755, 1179, 1208
MaeII ACGT 3 cut(s) 509, 1063, 1091
MaeIII GTNAC 3 cut(s) 559, 685, 709
MalI GATC 8 cut(s) 405, 419, 456, 657, 769, 810, 963, 1059
MboI GATC 8 cut(s) 403, 417, 454, 655, 767, 808, 961, 1057
MboII GAAGA 4 cut(s) 1013, 1063, 1144, 1147
MfeI CAATTG 1 cut(s) 425
MflI RGATCY 1 cut(s) 767
MhlI GDGCHC 1 cut(s) 1086
MluCI AATT 5 cut(s) 40, 86, 91, 425, 761
MlyI GAGTC 1 cut(s) 713
MmeI TCCRAC 2 cut(s) 966, 1074
MroI TCCGGA 1 cut(s) 224
MseI TTAA 6 cut(s) 369, 606, 732, 822, 1047, 1140
MslI CAYNNNNRTG 8 cut(s) 362, 597, 732, 867, 885, 900, 906, 912
MspI CCGG 5 cut(s) 82, 96, 194, 210, 225
MspR9I CCNGG 7 cut(s) 82, 83, 111, 121, 194, 1076, 1107
MunI CAATTG 1 cut(s) 425
Mva1269I GAATGC 1 cut(s) 169
MvaI CCWGG 4 cut(s) 111, 121, 1076, 1107
MvnI CGCG 1 cut(s) 613
MwoI GCNNNNNNNGC 3 cut(s) 586, 751, 1110
NciI CCSGG 3 cut(s) 82, 83, 194
NdeII GATC 8 cut(s) 403, 417, 454, 655, 767, 808, 961, 1057
NheI GCTAGC 1 cut(s) 754
NlaIV GGNNCC 5 cut(s) 223, 955, 1054, 1122, 1123
NmeAIII GCCGAG 1 cut(s) 973
NmuCI GTSAC 2 cut(s) 685, 709
NspI RCATGY 1 cut(s) 542
PagI TCATGA 1 cut(s) 901
PceI AGGCCT 1 cut(s) 480
PciI ACATGT 1 cut(s) 538
PctI GAATGC 1 cut(s) 169
PfeI GAWTC 3 cut(s) 12, 917, 1165
PflFI GACNNNGTC 1 cut(s) 359
PfoI TCCNGGA 1 cut(s) 119
PkrI GCNGC 1 cut(s) 1103
PleI GAGTC 1 cut(s) 712
PpsI GAGTC 1 cut(s) 712
PpuMI RGGWCCY 1 cut(s) 1121
PscI ACATGT 1 cut(s) 538
PshBI ATTAAT 2 cut(s) 732, 822
Psp1406I AACGTT 2 cut(s) 509, 1063
Psp5II RGGWCCY 1 cut(s) 1121
Psp6I CCWGG 4 cut(s) 109, 119, 1074, 1105
PspEI GGTNACC 1 cut(s) 685
PspGI CCWGG 4 cut(s) 109, 119, 1074, 1105
PspN4I GGNNCC 5 cut(s) 223, 955, 1054, 1122, 1123
PspPI GGNCC 1 cut(s) 1121
PspPPI RGGWCCY 1 cut(s) 1121
PsuI RGATCY 1 cut(s) 767
PsyI GACNNNGTC 1 cut(s) 359
RsaI GTAC 3 cut(s) 391, 912, 1022
RsaNI GTAC 3 cut(s) 390, 911, 1021
RseI CAYNNNNRTG 8 cut(s) 362, 597, 732, 867, 885, 900, 906, 912
SaqAI TTAA 6 cut(s) 369, 606, 732, 822, 1047, 1140
SatI GCNGC 1 cut(s) 1102
Sau3AI GATC 8 cut(s) 403, 417, 454, 655, 767, 808, 961, 1057
Sau96I GGNCC 1 cut(s) 1121
SchI GAGTC 1 cut(s) 713
ScrFI CCNGG 7 cut(s) 82, 83, 111, 121, 194, 1076, 1107
SduI GDGCHC 1 cut(s) 1086
SinI GGWCC 1 cut(s) 1121
SmaI CCCGGG 1 cut(s) 83
SmiMI CAYNNNNRTG 8 cut(s) 362, 597, 732, 867, 885, 900, 906, 912
SmlI CTYRAG 2 cut(s) 22, 928
SmoI CTYRAG 2 cut(s) 22, 928
SpeI ACTAGT 1 cut(s) 1178
Sse9I AATT 5 cut(s) 40, 86, 91, 425, 761
SseBI AGGCCT 1 cut(s) 480
SsiI CCGC 2 cut(s) 281, 611
SspMI CTAG 6 cut(s) 333, 407, 527, 755, 1179, 1208
StuI AGGCCT 1 cut(s) 480
StyD4I CCNGG 7 cut(s) 80, 81, 109, 119, 192, 1074, 1105
StyI CCWWGG 1 cut(s) 171
TaaI ACNGT 3 cut(s) 394, 925, 1052
TaiI ACGT 3 cut(s) 512, 1066, 1094
TaqI TCGA 5 cut(s) 8, 156, 180, 288, 347
TaqII GACCGA 1 cut(s) 704
TasI AATT 5 cut(s) 40, 86, 91, 425, 761
TatI WGTACW 3 cut(s) 389, 910, 1020
TfiI GAWTC 3 cut(s) 12, 917, 1165
Tru1I TTAA 6 cut(s) 369, 606, 732, 822, 1047, 1140
Tru9I TTAA 6 cut(s) 369, 606, 732, 822, 1047, 1140
TscAI CASTG 2 cut(s) 714, 928
TseFI GTSAC 2 cut(s) 685, 709
TseI GCWGC 1 cut(s) 1101
Tsp45I GTSAC 2 cut(s) 685, 709
TspDTI ATGAA 7 cut(s) 129, 615, 843, 885, 1043, 1144, 1188
TspGWI ACGGA 2 cut(s) 289, 710
TspMI CCCGGG 1 cut(s) 81
TspRI CASTG 2 cut(s) 714, 928
Tth111I GACNNNGTC 1 cut(s) 359
VneI GTGCAC 1 cut(s) 1082
VpaK11BI GGWCC 1 cut(s) 1121
VspI ATTAAT 2 cut(s) 732, 822
XapI RAATTY 2 cut(s) 86, 761
XceI RCATGY 1 cut(s) 542
XcmI CCANNNNNNNNNTGG 1 cut(s) 1113
XmaI CCCGGG 1 cut(s) 81
XspI CTAG 6 cut(s) 333, 407, 527, 755, 1179, 1208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.