Rroxscaffold_2G00110250

COBW domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001734
Physical Location & Seq
Reverse (-)
34460922 .. 34465932
5011 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00110250.1

Sequence Viewer

Length: 1374 bp
ATGGCTTCCATATCGGCCGACCTAGCCGCCACCCTTCTCTCCCTAACTTCCCGTCACTCCACCCGGGTCCGAGCCCCGGCCCTATTCCCTCTCACATGGAGACCCCGGACTTCCCCACTGTCCCATACCCTCAGCTACTCCACTTCAACTTCCTCCTTTCGCCAGAGACTCGTCTCCAACTCTAAGCAGGGCTCCAGAAGGTTCACTGTGGCCGCCACCACTGCCACGCCACCGCAGAGTGACAGCTCCGACGTTTTGACCCAGATTCCGCCGGATAATCGTATCCCGGCGACCATAATTACCGGCTTCTTGGGCTCCGGAAAGACTACATTGCTTAATCATATATTGACTGCCGACCACGGGAAGCGAATTGCAGTCATTGAAAATGAGTATGGTGAAGTAGACATTGATGGTTCTTTGGTGGCTGCGAAATCTGCCGGGGCTGAAGATATTGTTATGTTGAATAATGGGTGCCTTTGCTGCACTGTGAGGGGTGATCTTGTGCGGATGATTTCGGACTTGGTTAATAAGAAGAAAGGGAGGTTTGATCATATTGTGATAGAGACTACAGGATTGGCAAATCCAGCACCGATAATTCAAACATTTTATGCGGAAGATCAGGTTTTCAATGATGTCAAGTTGGACGGTGTTGTCACGTTAGTTGATGCTAAACATGCTCGACTTCATCTGGATGAAGTCAAGGCAGAAGGAGTGGTTAATGAGGCAGTAGAGCAGATTGCTTATGCTGATCGTATCATTGTGAATAAGACTGATCTTGTTGGCGAGTCAGATATTGCTTCTTTGGTGCAGAGAATAAGGAACATTAATGGCATGGCTCAACTGAAGCGTACAGAGTTTGGAAAAGTTAACCTGGATTATGTGCTCGGTATCGGAGGATTTGATTTGGAGAGGATAGAGAGTGCTATTGATGCTGAAGCTGAGAAGGAAGATCATGACCATCATCACGACCACCATGACCACCATGACCATCATGATCATCATCACCATCATGATGAAGAACATGATCACAAACATGAACATCATGATGGTCACCACTCCCATGATCATGTTCATGATCCTGGTGTTTCTTCTGTCAGTATTGTTTGCGAAGGGAACCTCGATCTGGAGAAGGCCAACATGTGGCTTGGCACACTGTTGTTGGACCGGAGTGAGGACATATATCGTATGAAAGGTCTTCTATCTGTTCAGGGTATGGATGAGAGATTTGTCTTCCAGGGAGTTCATGATATCTTCCAAGGTTCTCCCGATCGGTTGTGGGGCCCCAATGAGCCAAGAACAAATAAGATTGTTTTCATAGGGAAGAACTTGGATGCACAAGAGTTGGAGAAGGGTTTTAAAGGCTGTTTACTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CobW_C cobW

Protein Analysis

457

Amino Acids

50.69

Weight (kDa)

5.83

Isoelectric Point (pI)

32.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cobW PF02492 96 - 280 3.1e-59 CobW/HypB/UreG, nucleotide-binding domain
CobW_C PF07683 362 - 456 9.7e-23 Cobalamin synthesis protein cobW C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 650
AccB1I GGYRCC 1 cut(s) 471
AccB7I CCANNNNNTGG 1 cut(s) 1140
AccI GTMKAC 1 cut(s) 402
AccIII TCCGGA 1 cut(s) 317
AciI CCGC 6 cut(s) 27, 213, 233, 269, 505, 611
AclWI GGATC 1 cut(s) 1070
AcoI YGGCCR 2 cut(s) 15, 210
AcuI CTGAAG 3 cut(s) 465, 863, 954
AfaI GTAC 1 cut(s) 850
AfiI CCNNNNNNNGG 5 cut(s) 76, 360, 1123, 1140, 1171
AflIII ACRYGT 1 cut(s) 1137
AgsI TTSAA 5 cut(s) 147, 383, 463, 599, 628
AjnI CCWGG 3 cut(s) 870, 1078, 1233
AluBI AGCT 3 cut(s) 135, 246, 938
AluI AGCT 3 cut(s) 135, 246, 938
Alw21I GWGCWC 1 cut(s) 885
Alw26I GTCTC 4 cut(s) 94, 160, 178, 557
AlwI GGATC 1 cut(s) 1070
Ama87I CYCGRG 1 cut(s) 63
Aor13HI TCCGGA 1 cut(s) 317
AoxI GGCC 5 cut(s) 15, 78, 210, 1131, 1279
ApaI GGGCCC 1 cut(s) 1283
ApeKI GCWGC 2 cut(s) 425, 480
AseI ATTAAT 1 cut(s) 825
AspS9I GGNCC 5 cut(s) 67, 79, 1162, 1279, 1280
AsuC2I CCSGG 6 cut(s) 64, 65, 77, 106, 287, 439
AsuHPI GGTGA 4 cut(s) 407, 506, 995, 1043
AvaI CYCGRG 1 cut(s) 63
AvaII GGWCC 2 cut(s) 67, 1162
BaeGI GKGCMC 1 cut(s) 1283
BanI GGYRCC 1 cut(s) 471
BanII GRGCYC 4 cut(s) 76, 194, 317, 1283
BbsI GAAGAC 2 cut(s) 1187, 1222
Bbv12I GWGCWC 1 cut(s) 885
BbvCI CCTCAGC 1 cut(s) 131
BbvI GCAGC 2 cut(s) 412, 467
BccI CCATC 5 cut(s) 404, 966, 996, 1014, 1040
BciT130I CCWGG 3 cut(s) 872, 1080, 1235
BciVI GTATCC 1 cut(s) 293
BclI TGATCA 4 cut(s) 547, 994, 1024, 1063
BcnI CCSGG 6 cut(s) 64, 65, 77, 106, 287, 439
BcoDI GTCTC 4 cut(s) 94, 160, 178, 557
BfaI CTAG 1 cut(s) 23
BfmI CTRYAG 1 cut(s) 567
BfuI GTATCC 1 cut(s) 293
BisI GCNGC 4 cut(s) 27, 213, 426, 481
BlsI GCNGC 4 cut(s) 28, 214, 427, 482
Bme1390I CCNGG 9 cut(s) 64, 65, 77, 106, 287, 439, 872, 1080, 1235
Bme18I GGWCC 2 cut(s) 67, 1162
BmeT110I CYCGRG 1 cut(s) 63
BmgT120I GGNCC 5 cut(s) 67, 79, 1162, 1279, 1280
BmiI GGNNCC 8 cut(s) 68, 193, 316, 473, 1115, 1280, 1281, 1282
BmrFI CCNGG 9 cut(s) 64, 65, 77, 106, 287, 439, 872, 1080, 1235
BmsI GCATC 3 cut(s) 655, 919, 1321
BpiI GAAGAC 2 cut(s) 1187, 1222
BpmI CTGGAG 2 cut(s) 178, 1145
Bpu10I CCTNAGC 1 cut(s) 131
BpuMI CCSGG 6 cut(s) 64, 65, 77, 106, 287, 439
BsaBI GATNNNNATC 1 cut(s) 999
BsaI GGTCTC 1 cut(s) 94
BsaJI CCNNGG 7 cut(s) 63, 75, 104, 358, 438, 1234, 1255
BsaWI WCCGGW 2 cut(s) 317, 1164
Bsc4I CCNNNNNNNGG 5 cut(s) 76, 360, 1123, 1140, 1171
Bse118I RCCGGY 1 cut(s) 302
Bse3DI GCAATG 1 cut(s) 329
Bse8I GATNNNNATC 1 cut(s) 999
BseAI TCCGGA 1 cut(s) 317
BseBI CCWGG 3 cut(s) 872, 1080, 1235
BseDI CCNNGG 7 cut(s) 63, 75, 104, 358, 438, 1234, 1255
BseGI GGATG 4 cut(s) 513, 697, 1222, 1336
BseJI GATNNNNATC 1 cut(s) 999
BseLI CCNNNNNNNGG 5 cut(s) 76, 360, 1123, 1140, 1171
BseMI GCAATG 1 cut(s) 329
BseMII CTCAG 2 cut(s) 145, 930
BseSI GKGCMC 1 cut(s) 1283
BseX3I CGGCCG 1 cut(s) 15
BseXI GCAGC 2 cut(s) 412, 467
BsgI GTGCAG 2 cut(s) 466, 827
Bsh1285I CGRYCG 2 cut(s) 18, 1270
BshFI GGCC 5 cut(s) 17, 80, 212, 1133, 1281
BshNI GGYRCC 1 cut(s) 471
BsiEI CGRYCG 2 cut(s) 18, 1270
BsiHKAI GWGCWC 1 cut(s) 885
BsiHKCI CYCGRG 1 cut(s) 63
BsiSI CCGG 9 cut(s) 64, 77, 106, 272, 287, 303, 318, 438, 1165
BslFI GGGAC 1 cut(s) 106
BslI CCNNNNNNNGG 5 cut(s) 76, 360, 1123, 1140, 1171
BsmAI GTCTC 4 cut(s) 94, 160, 178, 557
BsmBI CGTCTC 1 cut(s) 178
BsmFI GGGAC 1 cut(s) 106
BsnI GGCC 5 cut(s) 17, 80, 212, 1133, 1281
Bso31I GGTCTC 1 cut(s) 94
BsoBI CYCGRG 1 cut(s) 63
Bsp120I GGGCCC 1 cut(s) 1279
Bsp1286I GDGCHC 5 cut(s) 76, 194, 317, 885, 1283
Bsp13I TCCGGA 1 cut(s) 317
BspACI CCGC 6 cut(s) 27, 213, 233, 269, 505, 611
BspANI GGCC 5 cut(s) 17, 80, 212, 1133, 1281
BspCNI CTCAG 2 cut(s) 144, 931
BspEI TCCGGA 1 cut(s) 317
BspHI TCATGA 6 cut(s) 952, 991, 1009, 1042, 1072, 1243
BspLI GGNNCC 8 cut(s) 68, 193, 316, 473, 1115, 1280, 1281, 1282
BspPI GGATC 1 cut(s) 1070
BspT107I GGYRCC 1 cut(s) 471
BspTNI GGTCTC 1 cut(s) 94
BsrDI GCAATG 1 cut(s) 329
BsrFI RCCGGY 1 cut(s) 302
BssAI RCCGGY 1 cut(s) 302
BssECI CCNNGG 7 cut(s) 63, 75, 104, 358, 438, 1234, 1255
BssT1I CCWWGG 1 cut(s) 1255
Bst2UI CCWGG 3 cut(s) 872, 1080, 1235
Bst4CI ACNGT 5 cut(s) 120, 208, 487, 647, 1155
BstDEI CTNAG 3 cut(s) 131, 183, 939
BstDSI CCRYGG 1 cut(s) 358
BstEII GGTNACC 1 cut(s) 1049
BstF5I GGATG 4 cut(s) 513, 697, 1222, 1336
BstMAI GTCTC 4 cut(s) 94, 160, 178, 557
BstMCI CGRYCG 2 cut(s) 18, 1270
BstMWI GCNNNNNNNGC 8 cut(s) 23, 221, 312, 434, 480, 584, 674, 929
BstNI CCWGG 3 cut(s) 872, 1080, 1235
BstNSI RCATGY 2 cut(s) 677, 1141
BstPI GGTNACC 1 cut(s) 1049
BstSCI CCNGG 9 cut(s) 62, 63, 75, 104, 285, 437, 870, 1078, 1233
BstSFI CTRYAG 1 cut(s) 567
BstSLI GKGCMC 1 cut(s) 1283
BstV1I GCAGC 2 cut(s) 412, 467
BstV2I GAAGAC 2 cut(s) 1187, 1222
BstZI CGGCCG 1 cut(s) 15
BsuI GTATCC 1 cut(s) 293
BsuRI GGCC 5 cut(s) 17, 80, 212, 1133, 1281
BtgI CCRYGG 1 cut(s) 358
BtsCI GGATG 4 cut(s) 513, 697, 1222, 1336
BtsI GCAGTG 1 cut(s) 219
BtsIMutI CAGTG 5 cut(s) 116, 204, 219, 483, 1151
CciI TCATGA 6 cut(s) 952, 991, 1009, 1042, 1072, 1243
Cfr10I RCCGGY 1 cut(s) 302
Cfr13I GGNCC 5 cut(s) 67, 79, 1162, 1279, 1280
Cfr9I CCCGGG 1 cut(s) 63
Csp6I GTAC 1 cut(s) 849
CviQI GTAC 1 cut(s) 849
DdeI CTNAG 3 cut(s) 131, 183, 939
DraI TTTAAA 1 cut(s) 1357
DrdI GACNNNNNNGTC 1 cut(s) 650
DseDI GACNNNNNNGTC 1 cut(s) 650
EaeI YGGCCR 2 cut(s) 15, 210
EagI CGGCCG 1 cut(s) 15
EciI GGCGGA 1 cut(s) 258
EclXI CGGCCG 1 cut(s) 15
Eco130I CCWWGG 1 cut(s) 1255
Eco24I GRGCYC 4 cut(s) 76, 194, 317, 1283
Eco31I GGTCTC 1 cut(s) 94
Eco32I GATATC 1 cut(s) 1249
Eco47I GGWCC 2 cut(s) 67, 1162
Eco52I CGGCCG 1 cut(s) 15
Eco57I CTGAAG 3 cut(s) 465, 863, 954
Eco88I CYCGRG 1 cut(s) 63
Eco91I GGTNACC 1 cut(s) 1049
EcoO109I RGGNCCY 2 cut(s) 1279, 1280
EcoO65I GGTNACC 1 cut(s) 1049
EcoRII CCWGG 3 cut(s) 870, 1078, 1233
EcoRV GATATC 1 cut(s) 1249
EcoT14I CCWWGG 1 cut(s) 1255
EcoT38I GRGCYC 4 cut(s) 76, 194, 317, 1283
ErhI CCWWGG 1 cut(s) 1255
Esp3I CGTCTC 1 cut(s) 178
FaqI GGGAC 1 cut(s) 106
FbaI TGATCA 4 cut(s) 547, 994, 1024, 1063
FblI GTMKAC 1 cut(s) 402
Fnu4HI GCNGC 4 cut(s) 27, 213, 426, 481
FokI GGATG 4 cut(s) 520, 704, 1229, 1343
FriOI GRGCYC 4 cut(s) 76, 194, 317, 1283
Fsp4HI GCNGC 4 cut(s) 27, 213, 426, 481
FspBI CTAG 1 cut(s) 23
GluI GCNGC 4 cut(s) 27, 213, 426, 481
GsuI CTGGAG 2 cut(s) 178, 1145
HaeIII GGCC 5 cut(s) 17, 80, 212, 1133, 1281
HapII CCGG 9 cut(s) 64, 77, 106, 272, 287, 303, 318, 438, 1165
HincII GTYRAC 1 cut(s) 868
HindII GTYRAC 1 cut(s) 868
HinfI GANTC 3 cut(s) 168, 265, 785
HpaI GTTAAC 1 cut(s) 868
HpaII CCGG 9 cut(s) 64, 77, 106, 272, 287, 303, 318, 438, 1165
HphI GGTGA 4 cut(s) 407, 506, 995, 1043
Hpy166II GTNNAC 4 cut(s) 204, 403, 868, 1367
Hpy188I TCNGA 5 cut(s) 71, 250, 517, 790, 893
Hpy8I GTNNAC 4 cut(s) 204, 403, 868, 1367
Hpy99I CGWCG 1 cut(s) 254
HpyAV CCTTC 7 cut(s) 44, 192, 701, 937, 1103, 1123, 1342
HpyCH4III ACNGT 5 cut(s) 120, 208, 487, 647, 1155
HpyCH4IV ACGT 2 cut(s) 252, 656
HpyCH4V TGCA 4 cut(s) 374, 483, 808, 1334
HpyF10VI GCNNNNNNNGC 8 cut(s) 23, 221, 312, 434, 480, 584, 674, 929
HpyF3I CTNAG 3 cut(s) 131, 183, 939
HpySE526I ACGT 2 cut(s) 252, 656
Kpn2I TCCGGA 1 cut(s) 317
Ksp22I TGATCA 4 cut(s) 547, 994, 1024, 1063
KspAI GTTAAC 1 cut(s) 868
LmnI GCTCC 3 cut(s) 197, 251, 320
Lsp1109I GCAGC 2 cut(s) 412, 467
LweI GCATC 3 cut(s) 655, 919, 1321
MaeI CTAG 1 cut(s) 23
MaeII ACGT 2 cut(s) 252, 656
MaeIII GTNAC 4 cut(s) 53, 239, 652, 1049
MhlI GDGCHC 5 cut(s) 76, 194, 317, 885, 1283
MluCI AATT 3 cut(s) 297, 369, 594
MlyI GAGTC 2 cut(s) 162, 794
MmeI TCCRAC 5 cut(s) 201, 273, 621, 1140, 1323
MroI TCCGGA 1 cut(s) 317
MseI TTAA 6 cut(s) 336, 525, 717, 825, 867, 1356
MslI CAYNNNNRTG 8 cut(s) 690, 1008, 1011, 1032, 1044, 1059, 1065, 1071
MspI CCGG 9 cut(s) 64, 77, 106, 272, 287, 303, 318, 438, 1165
MspR9I CCNGG 9 cut(s) 64, 65, 77, 106, 287, 439, 872, 1080, 1235
MvaI CCWGG 3 cut(s) 872, 1080, 1235
MwoI GCNNNNNNNGC 8 cut(s) 23, 221, 312, 434, 480, 584, 674, 929
NciI CCSGG 6 cut(s) 64, 65, 77, 106, 287, 439
NlaIV GGNNCC 8 cut(s) 68, 193, 316, 473, 1115, 1280, 1281, 1282
NmuCI GTSAC 4 cut(s) 53, 239, 652, 1049
NspI RCATGY 2 cut(s) 677, 1141
PagI TCATGA 6 cut(s) 952, 991, 1009, 1042, 1072, 1243
PciI ACATGT 1 cut(s) 1137
PfeI GAWTC 1 cut(s) 265
PflMI CCANNNNNTGG 1 cut(s) 1140
PkrI GCNGC 4 cut(s) 28, 214, 427, 482
Ple19I CGATCG 1 cut(s) 1270
PleI GAGTC 2 cut(s) 162, 793
PpsI GAGTC 2 cut(s) 162, 793
PscI ACATGT 1 cut(s) 1137
PshBI ATTAAT 1 cut(s) 825
Psp6I CCWGG 3 cut(s) 870, 1078, 1233
PspEI GGTNACC 1 cut(s) 1049
PspGI CCWGG 3 cut(s) 870, 1078, 1233
PspN4I GGNNCC 8 cut(s) 68, 193, 316, 473, 1115, 1280, 1281, 1282
PspOMI GGGCCC 1 cut(s) 1279
PspPI GGNCC 5 cut(s) 67, 79, 1162, 1279, 1280
PvuI CGATCG 1 cut(s) 1270
RsaI GTAC 1 cut(s) 850
RsaNI GTAC 1 cut(s) 849
RseI CAYNNNNRTG 8 cut(s) 690, 1008, 1011, 1032, 1044, 1059, 1065, 1071
SaqAI TTAA 6 cut(s) 336, 525, 717, 825, 867, 1356
SatI GCNGC 4 cut(s) 27, 213, 426, 481
Sau96I GGNCC 5 cut(s) 67, 79, 1162, 1279, 1280
SchI GAGTC 2 cut(s) 162, 794
ScrFI CCNGG 9 cut(s) 64, 65, 77, 106, 287, 439, 872, 1080, 1235
SduI GDGCHC 5 cut(s) 76, 194, 317, 885, 1283
SfaNI GCATC 3 cut(s) 655, 919, 1321
SfcI CTRYAG 1 cut(s) 567
SinI GGWCC 2 cut(s) 67, 1162
SmaI CCCGGG 1 cut(s) 65
SmiMI CAYNNNNRTG 8 cut(s) 690, 1008, 1011, 1032, 1044, 1059, 1065, 1071
Sse9I AATT 3 cut(s) 297, 369, 594
SsiI CCGC 6 cut(s) 27, 213, 233, 269, 505, 611
SspMI CTAG 1 cut(s) 23
StyD4I CCNGG 9 cut(s) 62, 63, 75, 104, 285, 437, 870, 1078, 1233
StyI CCWWGG 1 cut(s) 1255
TaaI ACNGT 5 cut(s) 120, 208, 487, 647, 1155
TaiI ACGT 2 cut(s) 255, 659
TaqI TCGA 2 cut(s) 679, 1119
TasI AATT 3 cut(s) 297, 369, 594
TauI GCSGC 2 cut(s) 29, 215
TfiI GAWTC 1 cut(s) 265
Tru1I TTAA 6 cut(s) 336, 525, 717, 825, 867, 1356
Tru9I TTAA 6 cut(s) 336, 525, 717, 825, 867, 1356
TscAI CASTG 5 cut(s) 123, 211, 226, 490, 1158
TseFI GTSAC 4 cut(s) 53, 239, 652, 1049
TseI GCWGC 2 cut(s) 425, 480
Tsp45I GTSAC 4 cut(s) 53, 239, 652, 1049
TspDTI ATGAA 8 cut(s) 674, 708, 1029, 1050, 1061, 1202, 1232, 1303
TspMI CCCGGG 1 cut(s) 63
TspRI CASTG 5 cut(s) 123, 211, 226, 490, 1158
Van91I CCANNNNNTGG 1 cut(s) 1140
VpaK11BI GGWCC 2 cut(s) 67, 1162
VspI ATTAAT 1 cut(s) 825
XceI RCATGY 2 cut(s) 677, 1141
XmaI CCCGGG 1 cut(s) 63
XmiI GTMKAC 1 cut(s) 402
XspI CTAG 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.