Rh7BG264900

COBW domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0001734
Physical Location & Seq
Forward (+)
24216432 .. 24220766
4335 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG264900.1

Sequence Viewer

Length: 1233 bp
ATGGCTACGAGAATCCTACTCTCAAGAACATCCCTCAAGAATTTCAATCACTTCCTTCAACCTTCTCTCCACAATTCCTCATCCTCCTCCTCCTCCTCCTCTCAATTCCTCAGAAGCCTCTGCTCTCTCGTTTCCCGAGAATTCAACTCCCGCCATGCCAAGAACCCTCATCCTGGGTATTCCAGGAGCATGGTCTCTGCCTCAAGCTACAATCTTGACGAGAATCCGACATTGGGTCTCGATACCCGCGTTCCGGCGACCATTATCACCGGCTTTCTCGGTTCCGGAAAGCGGATTGCGGTGATAGAAAATGAGTTTGGTGAGGTGGATATTGATGGGTCATTGGTTGCTAGTCATTCATCATCAAATGAAGACATTGTCATGGTTAATAATGGTTGCCTATGCTGTACTGTTCGGGGTGATCTAGTAAAAATGCTCTTGCAATTGGTTAAGGAAAAGCGAGACAAGTTTGATCATATTGTCATAGAAACCACAGGCCTCGCTAAGCCATCTCCTGTTATAGAAACGTTTTGTATGGATGAGCTAGTTTCACGATATGTGAAACTGGATGGAGTTGTTACTTTGGTGGATTGCAAGCATGCAATGCAGCATCTGAATGAAGTTAAACCAAGATTTGTGGTGAATGAGGCAGTAGAACAAGTTGCATATGCTGATCGTATTATATTGAACAAGATAGATTTGGTAACCGAGGCGGAGTTAGAGACATTGACCAAGAAAATCAAGCATATCAATGGGATGGCGCAAATCAAGCTAGCTAAATTTGGATCCGTGGACATGGACTTTGTTCTGGGTGTGGGAGGATATGATCTCGAAAGGATTGATAATGAAGTTCAAGTCGATAGTTCCTGTACTTCCTCACATCACCATGAAGCTGGACATGAACATGACAACCATCACCACGACCATGTACATGATTCTACTGTCTCAAGTGTCAGTATTGTTGCTGAGGGAACCCTTGATCTTGATGAGGTGGATGACTGGTTGGAAAGACTTATTGAAGAAAAGGGGGAGGACTTGTACAGGATGAAGGGCGTTTTATGTGTTAACGGTTCTGATCAACGTTATGTTTTCCAGGGAGTGCACTCTACTTTGGATGGGTGCCCAGGAGGCACATGGGGACCCGATGAGAAGAGGATTAACAAGCTTGTGTTCATAGGAAGGAATCTGGATGAAACTAGTCTTAGGAAAGGTTTCAGAGGTTGTCTAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CobW_C cobW

Protein Analysis

410

Amino Acids

45.46

Weight (kDa)

5.71

Isoelectric Point (pI)

38.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cobW PF02492 86 - 256 7.4e-43 CobW/HypB/UreG, nucleotide-binding domain
CobW_C PF07683 315 - 409 6e-24 Cobalamin synthesis protein cobW C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1119
AccII CGCG 1 cut(s) 249
AccIII TCCGGA 1 cut(s) 284
AciI CCGC 5 cut(s) 151, 247, 292, 299, 713
AclI AACGTT 2 cut(s) 527, 1081
AclWI GGATC 2 cut(s) 780, 793
AcsI RAATTY 3 cut(s) 40, 140, 779
AfaI GTAC 4 cut(s) 409, 871, 930, 1040
AfiI CCNNNNNNNGG 4 cut(s) 173, 233, 253, 291
AgsI TTSAA 6 cut(s) 46, 59, 145, 688, 854, 1019
AhdI GACNNNNNGTC 1 cut(s) 234
AhlI ACTAGT 1 cut(s) 1196
AjnI CCWGG 4 cut(s) 172, 182, 1092, 1123
AjuI GAANNNNNNNTTGG 4 cut(s) 215, 247, 300, 332
AluBI AGCT 6 cut(s) 207, 544, 772, 776, 893, 1165
AluI AGCT 6 cut(s) 207, 544, 772, 776, 893, 1165
Alw21I GWGCWC 1 cut(s) 1104
Alw26I GTCTC 5 cut(s) 199, 242, 456, 716, 949
Alw44I GTGCAC 1 cut(s) 1100
AlwI GGATC 2 cut(s) 780, 793
AlwNI CAGNNNCTG 1 cut(s) 613
Ama87I CYCGRG 1 cut(s) 135
Aor13HI TCCGGA 1 cut(s) 284
AoxI GGCC 1 cut(s) 496
ApaLI GTGCAC 1 cut(s) 1100
ApeKI GCWGC 1 cut(s) 607
ApoI RAATTY 3 cut(s) 40, 140, 779
ArsI GACNNNNNNTTYG 2 cut(s) 785, 817
Asp700I GAANNNNTTC 1 cut(s) 1211
AspLEI GCGC 1 cut(s) 763
AspS9I GGNCC 1 cut(s) 1139
AsuHPI GGTGA 7 cut(s) 259, 313, 332, 431, 652, 875, 908
AsuNHI GCTAGC 1 cut(s) 772
AvaI CYCGRG 1 cut(s) 135
AvaII GGWCC 1 cut(s) 1139
BaeGI GKGCMC 2 cut(s) 1104, 1124
BaeI ACNNNNGTAYC 2 cut(s) 234, 267
BamHI GGATCC 1 cut(s) 785
BanI GGYRCC 1 cut(s) 1119
BbsI GAAGAC 1 cut(s) 378
Bbv12I GWGCWC 1 cut(s) 1104
BbvCI CCTCAGC 1 cut(s) 966
BbvI GCAGC 1 cut(s) 619
BccI CCATC 6 cut(s) 329, 517, 563, 751, 921, 1109
BciT130I CCWGG 4 cut(s) 174, 184, 1094, 1125
BclI TGATCA 2 cut(s) 472, 1075
BcoDI GTCTC 5 cut(s) 199, 242, 456, 716, 949
BcuI ACTAGT 1 cut(s) 1196
BfaI CTAG 6 cut(s) 351, 425, 545, 773, 1197, 1226
BglI GCCNNNNNGGC 1 cut(s) 1128
BisI GCNGC 1 cut(s) 608
BlpI GCTNAGC 1 cut(s) 504
BlsI GCNGC 1 cut(s) 609
Bme1390I CCNGG 4 cut(s) 174, 184, 1094, 1125
Bme18I GGWCC 1 cut(s) 1139
BmeRI GACNNNNNGTC 1 cut(s) 234
BmeT110I CYCGRG 1 cut(s) 135
BmgT120I GGNCC 1 cut(s) 1139
BmiI GGNNCC 6 cut(s) 283, 787, 973, 1121, 1140, 1141
BmrFI CCNGG 4 cut(s) 174, 184, 1094, 1125
BmsI GCATC 1 cut(s) 619
BmtI GCTAGC 1 cut(s) 776
BpiI GAAGAC 1 cut(s) 378
Bpu10I CCTNAGC 1 cut(s) 966
Bpu1102I GCTNAGC 1 cut(s) 504
BpuEI CTTGAG 4 cut(s) 7, 20, 187, 931
BsaI GGTCTC 2 cut(s) 199, 242
BsaJI CCNNGG 5 cut(s) 173, 708, 789, 1093, 1123
BsaWI WCCGGW 1 cut(s) 284
BsaXI ACNNNNNCTCC 4 cut(s) 51, 81, 1022, 1052
Bsc4I CCNNNNNNNGG 4 cut(s) 173, 233, 253, 291
Bse118I RCCGGY 1 cut(s) 269
Bse1I ACTGG 2 cut(s) 570, 1004
Bse3DI GCAATG 1 cut(s) 609
BseAI TCCGGA 1 cut(s) 284
BseBI CCWGG 4 cut(s) 174, 184, 1094, 1125
BseDI CCNNGG 5 cut(s) 173, 708, 789, 1093, 1123
BseLI CCNNNNNNNGG 4 cut(s) 173, 233, 253, 291
BseMI GCAATG 1 cut(s) 609
BseMII CTCAG 2 cut(s) 124, 957
BseNI ACTGG 2 cut(s) 570, 1004
BseRI GAGGAG 5 cut(s) 76, 79, 82, 85, 88
BseSI GKGCMC 2 cut(s) 1104, 1124
BseXI GCAGC 1 cut(s) 619
Bsh1236I CGCG 1 cut(s) 249
BshFI GGCC 1 cut(s) 498
BshNI GGYRCC 1 cut(s) 1119
BsiHKAI GWGCWC 1 cut(s) 1104
BsiHKCI CYCGRG 1 cut(s) 135
BsiSI CCGG 3 cut(s) 254, 270, 285
BslFI GGGAC 1 cut(s) 1152
BslI CCNNNNNNNGG 4 cut(s) 173, 233, 253, 291
BsmAI GTCTC 5 cut(s) 199, 242, 456, 716, 949
BsmFI GGGAC 1 cut(s) 1152
BsnI GGCC 1 cut(s) 498
Bso31I GGTCTC 2 cut(s) 199, 242
BsoBI CYCGRG 1 cut(s) 135
Bsp1286I GDGCHC 2 cut(s) 1104, 1124
Bsp13I TCCGGA 1 cut(s) 284
Bsp1407I TGTACA 2 cut(s) 928, 1038
Bsp143I GATC 7 cut(s) 421, 472, 673, 785, 826, 979, 1075
Bsp1720I GCTNAGC 1 cut(s) 504
BspACI CCGC 5 cut(s) 151, 247, 292, 299, 713
BspANI GGCC 1 cut(s) 498
BspCNI CTCAG 2 cut(s) 123, 958
BspEI TCCGGA 1 cut(s) 284
BspFNI CGCG 1 cut(s) 249
BspLI GGNNCC 6 cut(s) 283, 787, 973, 1121, 1140, 1141
BspOI GCTAGC 1 cut(s) 776
BspPI GGATC 2 cut(s) 780, 793
BspT107I GGYRCC 1 cut(s) 1119
BspTNI GGTCTC 2 cut(s) 199, 242
BsrDI GCAATG 1 cut(s) 609
BsrFI RCCGGY 1 cut(s) 269
BsrGI TGTACA 2 cut(s) 928, 1038
BsrI ACTGG 2 cut(s) 570, 1004
BssAI RCCGGY 1 cut(s) 269
BssECI CCNNGG 5 cut(s) 173, 708, 789, 1093, 1123
BssMI GATC 7 cut(s) 421, 472, 673, 785, 826, 979, 1075
Bst2UI CCWGG 4 cut(s) 174, 184, 1094, 1125
Bst4CI ACNGT 3 cut(s) 412, 943, 1070
Bst6I CTCTTC 1 cut(s) 1145
BstAPI GCANNNNNTGC 1 cut(s) 604
BstAUI TGTACA 2 cut(s) 928, 1038
BstC8I GCNNGC 3 cut(s) 596, 600, 774
BstDEI CTNAG 4 cut(s) 110, 504, 966, 1202
BstDSI CCRYGG 1 cut(s) 789
BstEII GGTNACC 1 cut(s) 703
BstFNI CGCG 1 cut(s) 249
BstHHI GCGC 1 cut(s) 763
BstKTI GATC 7 cut(s) 424, 475, 676, 788, 829, 982, 1078
BstMAI GTCTC 5 cut(s) 199, 242, 456, 716, 949
BstMBI GATC 7 cut(s) 421, 472, 673, 785, 826, 979, 1075
BstMWI GCNNNNNNNGC 3 cut(s) 604, 769, 1128
BstNI CCWGG 4 cut(s) 174, 184, 1094, 1125
BstNSI RCATGY 1 cut(s) 602
BstPI GGTNACC 1 cut(s) 703
BstSCI CCNGG 4 cut(s) 172, 182, 1092, 1123
BstSLI GKGCMC 2 cut(s) 1104, 1124
BstUI CGCG 1 cut(s) 249
BstV1I GCAGC 1 cut(s) 619
BstV2I GAAGAC 1 cut(s) 378
BstX2I RGATCY 1 cut(s) 785
BstXI CCANNNNNNTGG 2 cut(s) 190, 893
BstYI RGATCY 1 cut(s) 785
BsuRI GGCC 1 cut(s) 498
BtgI CCRYGG 1 cut(s) 789
Cac8I GCNNGC 3 cut(s) 596, 600, 774
CaiI CAGNNNCTG 1 cut(s) 613
CfoI GCGC 1 cut(s) 763
Cfr10I RCCGGY 1 cut(s) 269
Cfr13I GGNCC 1 cut(s) 1139
Csp6I GTAC 4 cut(s) 408, 870, 929, 1039
CspCI CAANNNNNGTGG 2 cut(s) 618, 653
CviQI GTAC 4 cut(s) 408, 870, 929, 1039
DdeI CTNAG 4 cut(s) 110, 504, 966, 1202
DpnI GATC 7 cut(s) 423, 474, 675, 787, 828, 981, 1077
DpnII GATC 7 cut(s) 421, 472, 673, 785, 826, 979, 1075
DriI GACNNNNNGTC 1 cut(s) 234
Eam1104I CTCTTC 1 cut(s) 1145
Eam1105I GACNNNNNGTC 1 cut(s) 234
EarI CTCTTC 1 cut(s) 1145
EciI GGCGGA 1 cut(s) 728
Eco147I AGGCCT 1 cut(s) 498
Eco31I GGTCTC 2 cut(s) 199, 242
Eco47I GGWCC 1 cut(s) 1139
Eco88I CYCGRG 1 cut(s) 135
Eco91I GGTNACC 1 cut(s) 703
EcoO109I RGGNCCY 1 cut(s) 1139
EcoO65I GGTNACC 1 cut(s) 703
EcoRI GAATTC 1 cut(s) 140
EcoRII CCWGG 4 cut(s) 172, 182, 1092, 1123
FaqI GGGAC 1 cut(s) 1152
FauI CCCGC 2 cut(s) 158, 254
FauNDI CATATG 1 cut(s) 667
FbaI TGATCA 2 cut(s) 472, 1075
Fnu4HI GCNGC 1 cut(s) 608
Fsp4HI GCNGC 1 cut(s) 608
FspBI CTAG 6 cut(s) 351, 425, 545, 773, 1197, 1226
GlaI GCGC 1 cut(s) 762
GluI GCNGC 1 cut(s) 608
HaeIII GGCC 1 cut(s) 498
HapII CCGG 3 cut(s) 254, 270, 285
HhaI GCGC 1 cut(s) 763
Hin6I GCGC 1 cut(s) 761
HinP1I GCGC 1 cut(s) 761
HincII GTYRAC 1 cut(s) 1066
HindII GTYRAC 1 cut(s) 1066
HindIII AAGCTT 1 cut(s) 1163
HinfI GANTC 4 cut(s) 12, 223, 935, 1183
HpaI GTTAAC 1 cut(s) 1066
HpaII CCGG 3 cut(s) 254, 270, 285
HphI GGTGA 7 cut(s) 259, 313, 332, 431, 652, 875, 908
Hpy166II GTNNAC 3 cut(s) 793, 1066, 1102
Hpy188I TCNGA 5 cut(s) 113, 228, 615, 1075, 1217
Hpy8I GTNNAC 3 cut(s) 793, 1066, 1102
HpyAV CCTTC 4 cut(s) 65, 72, 1042, 1173
HpyCH4III ACNGT 3 cut(s) 412, 943, 1070
HpyCH4IV ACGT 2 cut(s) 527, 1081
HpyCH4V TGCA 6 cut(s) 442, 594, 602, 607, 665, 1102
HpyF10VI GCNNNNNNNGC 3 cut(s) 604, 769, 1128
HpyF3I CTNAG 4 cut(s) 110, 504, 966, 1202
HpySE526I ACGT 2 cut(s) 527, 1081
HspAI GCGC 1 cut(s) 761
KflI GGGWCCC 1 cut(s) 1139
Kpn2I TCCGGA 1 cut(s) 284
Ksp22I TGATCA 2 cut(s) 472, 1075
KspAI GTTAAC 1 cut(s) 1066
Kzo9I GATC 7 cut(s) 421, 472, 673, 785, 826, 979, 1075
LmnI GCTCC 1 cut(s) 186
Lsp1109I GCAGC 1 cut(s) 619
LweI GCATC 1 cut(s) 619
MaeI CTAG 6 cut(s) 351, 425, 545, 773, 1197, 1226
MaeII ACGT 2 cut(s) 527, 1081
MaeIII GTNAC 2 cut(s) 577, 703
MalI GATC 7 cut(s) 423, 474, 675, 787, 828, 981, 1077
MboI GATC 7 cut(s) 421, 472, 673, 785, 826, 979, 1075
MboII GAAGA 3 cut(s) 383, 1031, 1162
MfeI CAATTG 1 cut(s) 443
MflI RGATCY 1 cut(s) 785
MhlI GDGCHC 2 cut(s) 1104, 1124
MluCI AATT 6 cut(s) 40, 73, 104, 140, 443, 779
MmeI TCCRAC 2 cut(s) 251, 984
MroI TCCGGA 1 cut(s) 284
MroXI GAANNNNTTC 1 cut(s) 1211
MseI TTAA 5 cut(s) 387, 450, 624, 1065, 1158
MslI CAYNNNNRTG 7 cut(s) 380, 615, 750, 885, 903, 924, 930
MspI CCGG 3 cut(s) 254, 270, 285
MspR9I CCNGG 4 cut(s) 174, 184, 1094, 1125
MunI CAATTG 1 cut(s) 443
MvaI CCWGG 4 cut(s) 174, 184, 1094, 1125
MvnI CGCG 1 cut(s) 249
MwoI GCNNNNNNNGC 3 cut(s) 604, 769, 1128
NdeI CATATG 1 cut(s) 667
NdeII GATC 7 cut(s) 421, 472, 673, 785, 826, 979, 1075
NheI GCTAGC 1 cut(s) 772
NlaIV GGNNCC 6 cut(s) 283, 787, 973, 1121, 1140, 1141
NspI RCATGY 1 cut(s) 602
PaeI GCATGC 1 cut(s) 602
PceI AGGCCT 1 cut(s) 498
PcsI WCGNNNNNNNCGW 1 cut(s) 246
PdmI GAANNNNTTC 1 cut(s) 1211
PfeI GAWTC 4 cut(s) 12, 223, 935, 1183
PflFI GACNNNGTC 1 cut(s) 377
PfoI TCCNGGA 1 cut(s) 182
PkrI GCNGC 1 cut(s) 609
PpuMI RGGWCCY 1 cut(s) 1139
Psp1406I AACGTT 2 cut(s) 527, 1081
Psp5II RGGWCCY 1 cut(s) 1139
Psp6I CCWGG 4 cut(s) 172, 182, 1092, 1123
PspEI GGTNACC 1 cut(s) 703
PspGI CCWGG 4 cut(s) 172, 182, 1092, 1123
PspN4I GGNNCC 6 cut(s) 283, 787, 973, 1121, 1140, 1141
PspPI GGNCC 1 cut(s) 1139
PspPPI RGGWCCY 1 cut(s) 1139
PstNI CAGNNNCTG 1 cut(s) 613
PsuI RGATCY 1 cut(s) 785
PsyI GACNNNGTC 1 cut(s) 377
RsaI GTAC 4 cut(s) 409, 871, 930, 1040
RsaNI GTAC 4 cut(s) 408, 870, 929, 1039
RseI CAYNNNNRTG 7 cut(s) 380, 615, 750, 885, 903, 924, 930
SaqAI TTAA 5 cut(s) 387, 450, 624, 1065, 1158
SatI GCNGC 1 cut(s) 608
Sau3AI GATC 7 cut(s) 421, 472, 673, 785, 826, 979, 1075
Sau96I GGNCC 1 cut(s) 1139
ScrFI CCNGG 4 cut(s) 174, 184, 1094, 1125
SduI GDGCHC 2 cut(s) 1104, 1124
SfaNI GCATC 1 cut(s) 619
SinI GGWCC 1 cut(s) 1139
SmiMI CAYNNNNRTG 7 cut(s) 380, 615, 750, 885, 903, 924, 930
SmlI CTYRAG 4 cut(s) 22, 35, 202, 946
SmoI CTYRAG 4 cut(s) 22, 35, 202, 946
SpeI ACTAGT 1 cut(s) 1196
SphI GCATGC 1 cut(s) 602
Sse9I AATT 6 cut(s) 40, 73, 104, 140, 443, 779
SseBI AGGCCT 1 cut(s) 498
SsiI CCGC 5 cut(s) 151, 247, 292, 299, 713
SspMI CTAG 6 cut(s) 351, 425, 545, 773, 1197, 1226
StuI AGGCCT 1 cut(s) 498
StyD4I CCNGG 4 cut(s) 172, 182, 1092, 1123
TaaI ACNGT 3 cut(s) 412, 943, 1070
TaiI ACGT 2 cut(s) 530, 1084
TaqI TCGA 3 cut(s) 240, 831, 858
TasI AATT 6 cut(s) 40, 73, 104, 140, 443, 779
TatI WGTACW 4 cut(s) 407, 869, 928, 1038
TfiI GAWTC 4 cut(s) 12, 223, 935, 1183
Tru1I TTAA 5 cut(s) 387, 450, 624, 1065, 1158
Tru9I TTAA 5 cut(s) 387, 450, 624, 1065, 1158
TseI GCWGC 1 cut(s) 607
TspDTI ATGAA 9 cut(s) 348, 384, 633, 861, 903, 915, 1061, 1162, 1206
TspGWI ACGGA 1 cut(s) 778
Tth111I GACNNNGTC 1 cut(s) 377
VneI GTGCAC 1 cut(s) 1100
VpaK11BI GGWCC 1 cut(s) 1139
XapI RAATTY 3 cut(s) 40, 140, 779
XceI RCATGY 1 cut(s) 602
XcmI CCANNNNNNNNNTGG 1 cut(s) 1131
XmnI GAANNNNTTC 1 cut(s) 1211
XspI CTAG 6 cut(s) 351, 425, 545, 773, 1197, 1226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.