Rmu_sc0009973.1_g000004

COBW domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0009973.1
OG0001734
Physical Location & Seq
Forward (+)
28122 .. 32516
4395 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0009973.1_g000004.1.cds

Sequence Viewer

Length: 1101 bp
atggctgcgagaatcctactctctgcctcaagctacaatcttgacgagaatccgagcttgggtgtcgatacccgtgttccggcgaccattatcaccggctttctcggttccggaaagactacattgctcaatcatatactaacatctcaacatggaaagcggattgcggtcatagaaaatgagtttggtgaggtggatattgatgggtcgttggttgctagtcattcatcatcaaatgaagacattgtcatggttaataatggttgcctatgctgtactgttcggggtgatctagtaaaaatgctcttgcaattggttaaggaaaagcgagacaagtttgatcatattgtcatagaaaccacaggcctcgctaagccatcacctgttatagaaacgttttgtatggatgagctagtttcacgacatgtgaaactggatggagttgttactttggtggattgcaagcatgcaatgcagcatctgaatgaagttaaaccaagatttgtggtgaatgaggcagtagaacaagttgcatatgctgatcgtattatattgaacaagatagatttggtaaccgaggcggagttagagacattgaccaagaaaatcaagcatatcaatgggatggcgcaaatcaagctagctaaatttggatctgtggacatggactttgttctgggtgtgggaggatatgatcttgaaaggattgataatgaagttcaagtcgatagttcctctacttcctcacatcaccatgaagctggacatgaacatgacaaacatcaccacgaccatgtacatgattctactgtctcaagtgtcagtattgttgccgagggaacccttgatcttgatgaggtggatgactggttggaaagacttattgaagaaaagggggaggacttgtacaggatgaagggcgttttatgtgttaacggttctgatcaacgttatgttttccagggagtgcactctacattggatgggtgcccaggaggcacatggggacccgatgagaagaggattaacaagcttgtgttcataggaaggaatctggatgaaactagtcttaggaaaggtttcagaggttgtctagcatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CobW_C cobW

Protein Analysis

366

Amino Acids

40.42

Weight (kDa)

5.4

Isoelectric Point (pI)

31.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 987
AccIII TCCGGA 1 cut(s) 110
AciI CCGC 3 cut(s) 160, 167, 581
AclI AACGTT 2 cut(s) 395, 949
AclWI GGATC 1 cut(s) 661
AcsI RAATTY 1 cut(s) 647
AfaI GTAC 3 cut(s) 277, 798, 908
AfiI CCNNNNNNNGG 2 cut(s) 59, 79
AflIII ACRYGT 1 cut(s) 424
AgsI TTSAA 4 cut(s) 556, 701, 722, 887
AhlI ACTAGT 1 cut(s) 1064
AjnI CCWGG 2 cut(s) 960, 991
AjuI GAANNNNNNNTTGG 4 cut(s) 41, 73, 168, 200
AluBI AGCT 7 cut(s) 33, 57, 412, 640, 644, 761, 1033
AluI AGCT 7 cut(s) 33, 57, 412, 640, 644, 761, 1033
Alw21I GWGCWC 1 cut(s) 972
Alw26I GTCTC 3 cut(s) 324, 584, 817
Alw44I GTGCAC 1 cut(s) 968
AlwI GGATC 1 cut(s) 661
AlwNI CAGNNNCTG 1 cut(s) 481
Aor13HI TCCGGA 1 cut(s) 110
AoxI GGCC 1 cut(s) 364
ApaLI GTGCAC 1 cut(s) 968
ApeKI GCWGC 2 cut(s) 5, 475
ApoI RAATTY 1 cut(s) 647
ArsI GACNNNNNNTTYG 2 cut(s) 653, 685
Asp700I GAANNNNTTC 1 cut(s) 1079
AspLEI GCGC 1 cut(s) 631
AspS9I GGNCC 1 cut(s) 1007
AsuHPI GGTGA 7 cut(s) 85, 200, 299, 372, 520, 743, 776
AsuNHI GCTAGC 1 cut(s) 640
AvaII GGWCC 1 cut(s) 1007
BaeGI GKGCMC 2 cut(s) 972, 992
BaeI ACNNNNGTAYC 2 cut(s) 60, 93
BanI GGYRCC 1 cut(s) 987
BbsI GAAGAC 1 cut(s) 246
Bbv12I GWGCWC 1 cut(s) 972
BbvI GCAGC 1 cut(s) 487
BccI CCATC 5 cut(s) 197, 385, 431, 619, 977
BciT130I CCWGG 2 cut(s) 962, 993
BclI TGATCA 2 cut(s) 340, 943
BcoDI GTCTC 3 cut(s) 324, 584, 817
BcuI ACTAGT 1 cut(s) 1064
BfaI CTAG 6 cut(s) 219, 293, 413, 641, 1065, 1094
BglI GCCNNNNNGGC 1 cut(s) 996
BisI GCNGC 2 cut(s) 6, 476
BlpI GCTNAGC 1 cut(s) 372
BlsI GCNGC 2 cut(s) 7, 477
Bme1390I CCNGG 2 cut(s) 962, 993
Bme18I GGWCC 1 cut(s) 1007
BmgT120I GGNCC 1 cut(s) 1007
BmiI GGNNCC 5 cut(s) 109, 841, 989, 1008, 1009
BmrFI CCNGG 2 cut(s) 962, 993
BmsI GCATC 1 cut(s) 487
BmtI GCTAGC 1 cut(s) 644
BpiI GAAGAC 1 cut(s) 246
Bpu1102I GCTNAGC 1 cut(s) 372
BpuEI CTTGAG 2 cut(s) 13, 799
BsaJI CCNNGG 4 cut(s) 576, 834, 961, 991
BsaWI WCCGGW 1 cut(s) 110
BsaXI ACNNNNNCTCC 2 cut(s) 890, 920
Bsc4I CCNNNNNNNGG 2 cut(s) 59, 79
Bse118I RCCGGY 1 cut(s) 95
Bse1I ACTGG 2 cut(s) 438, 872
Bse3DI GCAATG 2 cut(s) 122, 477
BseAI TCCGGA 1 cut(s) 110
BseBI CCWGG 2 cut(s) 962, 993
BseDI CCNNGG 4 cut(s) 576, 834, 961, 991
BseGI GGATG 7 cut(s) 412, 442, 630, 868, 918, 988, 1063
BseLI CCNNNNNNNGG 2 cut(s) 59, 79
BseMI GCAATG 2 cut(s) 122, 477
BseNI ACTGG 2 cut(s) 438, 872
BseSI GKGCMC 2 cut(s) 972, 992
BseXI GCAGC 1 cut(s) 487
BshFI GGCC 1 cut(s) 366
BshNI GGYRCC 1 cut(s) 987
BsiHKAI GWGCWC 1 cut(s) 972
BsiSI CCGG 3 cut(s) 80, 96, 111
BslFI GGGAC 1 cut(s) 1020
BslI CCNNNNNNNGG 2 cut(s) 59, 79
BsmAI GTCTC 3 cut(s) 324, 584, 817
BsmFI GGGAC 1 cut(s) 1020
BsnI GGCC 1 cut(s) 366
Bsp1286I GDGCHC 2 cut(s) 972, 992
Bsp13I TCCGGA 1 cut(s) 110
Bsp1407I TGTACA 2 cut(s) 796, 906
Bsp143I GATC 7 cut(s) 289, 340, 541, 653, 694, 847, 943
Bsp1720I GCTNAGC 1 cut(s) 372
BspACI CCGC 3 cut(s) 160, 167, 581
BspANI GGCC 1 cut(s) 366
BspEI TCCGGA 1 cut(s) 110
BspLI GGNNCC 5 cut(s) 109, 841, 989, 1008, 1009
BspOI GCTAGC 1 cut(s) 644
BspPI GGATC 1 cut(s) 661
BspT107I GGYRCC 1 cut(s) 987
BsrDI GCAATG 2 cut(s) 122, 477
BsrFI RCCGGY 1 cut(s) 95
BsrGI TGTACA 2 cut(s) 796, 906
BsrI ACTGG 2 cut(s) 438, 872
BssAI RCCGGY 1 cut(s) 95
BssECI CCNNGG 4 cut(s) 576, 834, 961, 991
BssMI GATC 7 cut(s) 289, 340, 541, 653, 694, 847, 943
Bst2UI CCWGG 2 cut(s) 962, 993
Bst4CI ACNGT 3 cut(s) 280, 811, 938
Bst6I CTCTTC 1 cut(s) 1013
BstAPI GCANNNNNTGC 1 cut(s) 472
BstAUI TGTACA 2 cut(s) 796, 906
BstC8I GCNNGC 3 cut(s) 464, 468, 642
BstDEI CTNAG 2 cut(s) 372, 1070
BstEII GGTNACC 1 cut(s) 571
BstF5I GGATG 7 cut(s) 412, 442, 630, 868, 918, 988, 1063
BstHHI GCGC 1 cut(s) 631
BstKTI GATC 7 cut(s) 292, 343, 544, 656, 697, 850, 946
BstMAI GTCTC 3 cut(s) 324, 584, 817
BstMBI GATC 7 cut(s) 289, 340, 541, 653, 694, 847, 943
BstMWI GCNNNNNNNGC 3 cut(s) 472, 637, 996
BstNI CCWGG 2 cut(s) 962, 993
BstNSI RCATGY 2 cut(s) 428, 470
BstPI GGTNACC 1 cut(s) 571
BstSCI CCNGG 2 cut(s) 960, 991
BstSLI GKGCMC 2 cut(s) 972, 992
BstV1I GCAGC 1 cut(s) 487
BstV2I GAAGAC 1 cut(s) 246
BstX2I RGATCY 1 cut(s) 653
BstXI CCANNNNNNTGG 1 cut(s) 761
BstYI RGATCY 1 cut(s) 653
BsuRI GGCC 1 cut(s) 366
BtsCI GGATG 7 cut(s) 412, 442, 630, 868, 918, 988, 1063
Cac8I GCNNGC 3 cut(s) 464, 468, 642
CaiI CAGNNNCTG 1 cut(s) 481
CfoI GCGC 1 cut(s) 631
Cfr10I RCCGGY 1 cut(s) 95
Cfr13I GGNCC 1 cut(s) 1007
Csp6I GTAC 3 cut(s) 276, 797, 907
CspCI CAANNNNNGTGG 2 cut(s) 486, 521
CviQI GTAC 3 cut(s) 276, 797, 907
DdeI CTNAG 2 cut(s) 372, 1070
DpnI GATC 7 cut(s) 291, 342, 543, 655, 696, 849, 945
DpnII GATC 7 cut(s) 289, 340, 541, 653, 694, 847, 943
Eam1104I CTCTTC 1 cut(s) 1013
EarI CTCTTC 1 cut(s) 1013
EciI GGCGGA 1 cut(s) 596
Eco147I AGGCCT 1 cut(s) 366
Eco47I GGWCC 1 cut(s) 1007
Eco91I GGTNACC 1 cut(s) 571
EcoO109I RGGNCCY 1 cut(s) 1007
EcoO65I GGTNACC 1 cut(s) 571
EcoRII CCWGG 2 cut(s) 960, 991
FaqI GGGAC 1 cut(s) 1020
FauNDI CATATG 1 cut(s) 535
FbaI TGATCA 2 cut(s) 340, 943
Fnu4HI GCNGC 2 cut(s) 6, 476
FokI GGATG 7 cut(s) 419, 449, 637, 875, 925, 995, 1070
Fsp4HI GCNGC 2 cut(s) 6, 476
FspBI CTAG 6 cut(s) 219, 293, 413, 641, 1065, 1094
GlaI GCGC 1 cut(s) 630
GluI GCNGC 2 cut(s) 6, 476
HaeIII GGCC 1 cut(s) 366
HapII CCGG 3 cut(s) 80, 96, 111
HhaI GCGC 1 cut(s) 631
Hin6I GCGC 1 cut(s) 629
HinP1I GCGC 1 cut(s) 629
HincII GTYRAC 1 cut(s) 934
HindII GTYRAC 1 cut(s) 934
HindIII AAGCTT 1 cut(s) 1031
HinfI GANTC 4 cut(s) 12, 49, 803, 1051
HpaI GTTAAC 1 cut(s) 934
HpaII CCGG 3 cut(s) 80, 96, 111
HphI GGTGA 7 cut(s) 85, 200, 299, 372, 520, 743, 776
Hpy166II GTNNAC 3 cut(s) 661, 934, 970
Hpy188I TCNGA 4 cut(s) 54, 483, 943, 1085
Hpy188III TCNNGA 6 cut(s) 41, 111, 420, 698, 851, 1055
Hpy8I GTNNAC 3 cut(s) 661, 934, 970
HpyAV CCTTC 2 cut(s) 910, 1041
HpyCH4III ACNGT 3 cut(s) 280, 811, 938
HpyCH4IV ACGT 2 cut(s) 395, 949
HpyCH4V TGCA 6 cut(s) 310, 462, 470, 475, 533, 970
HpyF10VI GCNNNNNNNGC 3 cut(s) 472, 637, 996
HpyF3I CTNAG 2 cut(s) 372, 1070
HpySE526I ACGT 2 cut(s) 395, 949
HspAI GCGC 1 cut(s) 629
KflI GGGWCCC 1 cut(s) 1007
Kpn2I TCCGGA 1 cut(s) 110
Ksp22I TGATCA 2 cut(s) 340, 943
KspAI GTTAAC 1 cut(s) 934
Kzo9I GATC 7 cut(s) 289, 340, 541, 653, 694, 847, 943
Lsp1109I GCAGC 1 cut(s) 487
LweI GCATC 1 cut(s) 487
MaeI CTAG 6 cut(s) 219, 293, 413, 641, 1065, 1094
MaeII ACGT 2 cut(s) 395, 949
MaeIII GTNAC 2 cut(s) 445, 571
MalI GATC 7 cut(s) 291, 342, 543, 655, 696, 849, 945
MboI GATC 7 cut(s) 289, 340, 541, 653, 694, 847, 943
MboII GAAGA 3 cut(s) 251, 899, 1030
MfeI CAATTG 1 cut(s) 311
MflI RGATCY 1 cut(s) 653
MhlI GDGCHC 2 cut(s) 972, 992
MluCI AATT 2 cut(s) 311, 647
MmeI TCCRAC 1 cut(s) 852
MroI TCCGGA 1 cut(s) 110
MroXI GAANNNNTTC 1 cut(s) 1079
MseI TTAA 5 cut(s) 255, 318, 492, 933, 1026
MslI CAYNNNNRTG 7 cut(s) 248, 483, 618, 753, 771, 792, 798
MspI CCGG 3 cut(s) 80, 96, 111
MspR9I CCNGG 2 cut(s) 962, 993
MunI CAATTG 1 cut(s) 311
MvaI CCWGG 2 cut(s) 962, 993
MwoI GCNNNNNNNGC 3 cut(s) 472, 637, 996
NdeI CATATG 1 cut(s) 535
NdeII GATC 7 cut(s) 289, 340, 541, 653, 694, 847, 943
NheI GCTAGC 1 cut(s) 640
NlaIV GGNNCC 5 cut(s) 109, 841, 989, 1008, 1009
NmeAIII GCCGAG 1 cut(s) 859
NspI RCATGY 2 cut(s) 428, 470
PaeI GCATGC 1 cut(s) 470
PceI AGGCCT 1 cut(s) 366
PciI ACATGT 1 cut(s) 424
PdmI GAANNNNTTC 1 cut(s) 1079
PfeI GAWTC 4 cut(s) 12, 49, 803, 1051
PflFI GACNNNGTC 1 cut(s) 245
PkrI GCNGC 2 cut(s) 7, 477
PpuMI RGGWCCY 1 cut(s) 1007
PscI ACATGT 1 cut(s) 424
Psp1406I AACGTT 2 cut(s) 395, 949
Psp5II RGGWCCY 1 cut(s) 1007
Psp6I CCWGG 2 cut(s) 960, 991
PspEI GGTNACC 1 cut(s) 571
PspGI CCWGG 2 cut(s) 960, 991
PspN4I GGNNCC 5 cut(s) 109, 841, 989, 1008, 1009
PspPI GGNCC 1 cut(s) 1007
PspPPI RGGWCCY 1 cut(s) 1007
PstNI CAGNNNCTG 1 cut(s) 481
PsuI RGATCY 1 cut(s) 653
PsyI GACNNNGTC 1 cut(s) 245
RsaI GTAC 3 cut(s) 277, 798, 908
RsaNI GTAC 3 cut(s) 276, 797, 907
RseI CAYNNNNRTG 7 cut(s) 248, 483, 618, 753, 771, 792, 798
SaqAI TTAA 5 cut(s) 255, 318, 492, 933, 1026
SatI GCNGC 2 cut(s) 6, 476
Sau3AI GATC 7 cut(s) 289, 340, 541, 653, 694, 847, 943
Sau96I GGNCC 1 cut(s) 1007
ScrFI CCNGG 2 cut(s) 962, 993
SduI GDGCHC 2 cut(s) 972, 992
SfaNI GCATC 1 cut(s) 487
SinI GGWCC 1 cut(s) 1007
SmiMI CAYNNNNRTG 7 cut(s) 248, 483, 618, 753, 771, 792, 798
SmlI CTYRAG 2 cut(s) 28, 814
SmoI CTYRAG 2 cut(s) 28, 814
SpeI ACTAGT 1 cut(s) 1064
SphI GCATGC 1 cut(s) 470
Sse9I AATT 2 cut(s) 311, 647
SseBI AGGCCT 1 cut(s) 366
SsiI CCGC 3 cut(s) 160, 167, 581
SspMI CTAG 6 cut(s) 219, 293, 413, 641, 1065, 1094
StuI AGGCCT 1 cut(s) 366
StyD4I CCNGG 2 cut(s) 960, 991
TaaI ACNGT 3 cut(s) 280, 811, 938
TaiI ACGT 2 cut(s) 398, 952
TaqI TCGA 2 cut(s) 66, 726
TasI AATT 2 cut(s) 311, 647
TatI WGTACW 3 cut(s) 275, 796, 906
TfiI GAWTC 4 cut(s) 12, 49, 803, 1051
Tru1I TTAA 5 cut(s) 255, 318, 492, 933, 1026
Tru9I TTAA 5 cut(s) 255, 318, 492, 933, 1026
TseI GCWGC 2 cut(s) 5, 475
TspDTI ATGAA 9 cut(s) 216, 252, 501, 729, 771, 783, 929, 1030, 1074
Tth111I GACNNNGTC 1 cut(s) 245
VneI GTGCAC 1 cut(s) 968
VpaK11BI GGWCC 1 cut(s) 1007
XapI RAATTY 1 cut(s) 647
XceI RCATGY 2 cut(s) 428, 470
XcmI CCANNNNNNNNNTGG 1 cut(s) 999
XmnI GAANNNNTTC 1 cut(s) 1079
XspI CTAG 6 cut(s) 219, 293, 413, 641, 1065, 1094
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.