RchiOBHm_Chr7g0212921

COBW domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001734
Physical Location & Seq
Forward (+)
30104452 .. 30110610
6159 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19056

Sequence Viewer

Length: 1263 bp
ATGGCTGCGAGAATCCTACTCTCAAGAACATCCCTCAAGAATTTCAATCACTTCCTTCATCCTCCTATCCACGATTCCTCATCCTCCTCTCAATTCCTCCGAAGCCTATGCTCCCTCGTTTCCCGACAATTCAATTCCCGCCATGCCAAGAACCCTCATCCTGGGTATTCCAGGAGCATGGTCTCTGCCTCAAGCTACAATCTTGACGAGAATCCGAGCTTGGATCTCGATACCCGTGTTCCGGCGACCATTATCACTGGCTTCCTCGGTTCCGGAAAGACTACATTGCTCAATCATATACTAACATCTCAACATGGCAAGCGGATTGCGGTGATAGAAAATGAGTTTGGTGAGGTGGATATTGATGGGTCATTGGTTGCTAGTCATTCATCATCAAATGAAGACATTGTCATGGTTAATAATGGTTGCCTGTGCTGTACTGTTCGGGGTGATCTGGTAAAAATGCTCTTGCAATTGGTTAAGGAAAAGCGAGAGAAGTTTGATCATATTGTCATAGAAACCACAGGCCTCGCTAAGCCATCTCCTGTTATAGAAACGTTTTGTATGGATGAGCTAGTTTCACGACATGTGAAACTGGATGGAGTTGTTACGTTGGTGGATTGCAAGCATGCAATGCAGCATCTGAATGAAGTTAAACCAAGATTTGTGGTGAATGAGGCAGTAGAACAAGTTGCATATGCTGATCGTATTATATTGAACAAGATAGATTTGGTAACCGAGGCGGAGTTAGAGACATTGACCAAGAAAATCAAGCATATCAATGGGATGGCGCAAATCAAGCTAGCTAAATTTGGATCCGTGGACATGGACTTTGTTCTGGGTGTGGGAGGATATGATCTCGAAAGGATTGATAATGAAGTTCAAGTCGATAGTTCCTGTACTTCCTCACATCACCATGAAGCTGGACATGAACATGACAACCATCACCATGACCATGTCCATGATTCTACTGTCTCAAGTGTCAGTATTGTTGCCGAGGGAACCCTTGATCTTGATGAGGTGGATGACTGGTTGGAAAGACTTATTGAAGAAAAGGGGGAGGACTTGTACAGGATGAAGGGCGTTTTATGTGTTAACGGTTCTGATCAACGTTATGTTTTCCAGGGAGTGCACTCTACATTGGATGGGTGCCCAGGAGGCACATGGGGACCCGATGAGAAGAGGATTAACAAGCTTGTGTTCATAGGAAGGAATCTGGATGAAACTAGTCTTAGGAAAGGTTTCAGAGGTTGTCTAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CobW_C cobW

Protein Analysis

420

Amino Acids

46.68

Weight (kDa)

5.87

Isoelectric Point (pI)

37.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cobW PF02492 81 - 266 2.8e-59 CobW/HypB/UreG, nucleotide-binding domain
CobW_C PF07683 325 - 419 6.3e-24 Cobalamin synthesis protein cobW C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1149
AccIII TCCGGA 1 cut(s) 272
AciI CCGC 4 cut(s) 139, 322, 329, 743
AclI AACGTT 2 cut(s) 557, 1111
AclWI GGATC 3 cut(s) 231, 810, 823
AcsI RAATTY 2 cut(s) 40, 809
AfaI GTAC 3 cut(s) 439, 901, 1070
AfiI CCNNNNNNNGG 2 cut(s) 161, 241
AflIII ACRYGT 1 cut(s) 586
AgsI TTSAA 5 cut(s) 46, 133, 718, 884, 1049
AhlI ACTAGT 1 cut(s) 1226
AjnI CCWGG 4 cut(s) 160, 170, 1122, 1153
AjuI GAANNNNNNNTTGG 4 cut(s) 203, 235, 330, 362
AluBI AGCT 7 cut(s) 195, 219, 574, 802, 806, 923, 1195
AluI AGCT 7 cut(s) 195, 219, 574, 802, 806, 923, 1195
Alw21I GWGCWC 1 cut(s) 1134
Alw26I GTCTC 3 cut(s) 187, 746, 979
Alw44I GTGCAC 1 cut(s) 1130
AlwI GGATC 3 cut(s) 231, 810, 823
AlwNI CAGNNNCTG 1 cut(s) 643
Aor13HI TCCGGA 1 cut(s) 272
AoxI GGCC 1 cut(s) 526
ApaLI GTGCAC 1 cut(s) 1130
ApeKI GCWGC 2 cut(s) 5, 637
ApoI RAATTY 2 cut(s) 40, 809
ArsI GACNNNNNNTTYG 2 cut(s) 815, 847
Asp700I GAANNNNTTC 1 cut(s) 1241
AspLEI GCGC 1 cut(s) 793
AspS9I GGNCC 1 cut(s) 1169
AsuHPI GGTGA 6 cut(s) 343, 362, 461, 682, 905, 938
AsuNHI GCTAGC 1 cut(s) 802
AvaII GGWCC 1 cut(s) 1169
BaeGI GKGCMC 2 cut(s) 1134, 1154
BaeI ACNNNNGTAYC 2 cut(s) 222, 255
BamHI GGATCC 1 cut(s) 815
BanI GGYRCC 1 cut(s) 1149
BbsI GAAGAC 1 cut(s) 408
Bbv12I GWGCWC 1 cut(s) 1134
BbvI GCAGC 1 cut(s) 649
BccI CCATC 6 cut(s) 359, 547, 593, 781, 951, 1139
BcgI CGANNNNNNTGC 2 cut(s) 90, 124
BciT130I CCWGG 4 cut(s) 162, 172, 1124, 1155
BclI TGATCA 2 cut(s) 502, 1105
BcoDI GTCTC 3 cut(s) 187, 746, 979
BcuI ACTAGT 1 cut(s) 1226
BfaI CTAG 5 cut(s) 381, 575, 803, 1227, 1256
BglI GCCNNNNNGGC 1 cut(s) 1158
BisI GCNGC 2 cut(s) 6, 638
BlpI GCTNAGC 1 cut(s) 534
BlsI GCNGC 2 cut(s) 7, 639
Bme1390I CCNGG 4 cut(s) 162, 172, 1124, 1155
Bme18I GGWCC 1 cut(s) 1169
BmgT120I GGNCC 1 cut(s) 1169
BmiI GGNNCC 6 cut(s) 271, 817, 1003, 1151, 1170, 1171
BmrFI CCNGG 4 cut(s) 162, 172, 1124, 1155
BmsI GCATC 1 cut(s) 649
BmtI GCTAGC 1 cut(s) 806
BpiI GAAGAC 1 cut(s) 408
Bpu1102I GCTNAGC 1 cut(s) 534
BpuEI CTTGAG 4 cut(s) 7, 20, 175, 961
BsaI GGTCTC 1 cut(s) 187
BsaJI CCNNGG 7 cut(s) 161, 265, 738, 819, 996, 1123, 1153
BsaWI WCCGGW 1 cut(s) 272
BsaXI ACNNNNNCTCC 2 cut(s) 1052, 1082
Bsc4I CCNNNNNNNGG 2 cut(s) 161, 241
Bse1I ACTGG 3 cut(s) 262, 600, 1034
Bse3DI GCAATG 2 cut(s) 284, 639
BseAI TCCGGA 1 cut(s) 272
BseBI CCWGG 4 cut(s) 162, 172, 1124, 1155
BseDI CCNNGG 7 cut(s) 161, 265, 738, 819, 996, 1123, 1153
BseLI CCNNNNNNNGG 2 cut(s) 161, 241
BseMI GCAATG 2 cut(s) 284, 639
BseNI ACTGG 3 cut(s) 262, 600, 1034
BseRI GAGGAG 1 cut(s) 76
BseSI GKGCMC 2 cut(s) 1134, 1154
BseXI GCAGC 1 cut(s) 649
BshFI GGCC 1 cut(s) 528
BshNI GGYRCC 1 cut(s) 1149
BsiHKAI GWGCWC 1 cut(s) 1134
BsiSI CCGG 2 cut(s) 242, 273
BslFI GGGAC 1 cut(s) 1182
BslI CCNNNNNNNGG 2 cut(s) 161, 241
BsmAI GTCTC 3 cut(s) 187, 746, 979
BsmFI GGGAC 1 cut(s) 1182
BsnI GGCC 1 cut(s) 528
Bso31I GGTCTC 1 cut(s) 187
Bsp1286I GDGCHC 2 cut(s) 1134, 1154
Bsp13I TCCGGA 1 cut(s) 272
Bsp1407I TGTACA 1 cut(s) 1068
Bsp143I GATC 8 cut(s) 223, 451, 502, 703, 815, 856, 1009, 1105
Bsp1720I GCTNAGC 1 cut(s) 534
BspACI CCGC 4 cut(s) 139, 322, 329, 743
BspANI GGCC 1 cut(s) 528
BspEI TCCGGA 1 cut(s) 272
BspLI GGNNCC 6 cut(s) 271, 817, 1003, 1151, 1170, 1171
BspOI GCTAGC 1 cut(s) 806
BspPI GGATC 3 cut(s) 231, 810, 823
BspT107I GGYRCC 1 cut(s) 1149
BspTNI GGTCTC 1 cut(s) 187
BsrDI GCAATG 2 cut(s) 284, 639
BsrGI TGTACA 1 cut(s) 1068
BsrI ACTGG 3 cut(s) 262, 600, 1034
BssECI CCNNGG 7 cut(s) 161, 265, 738, 819, 996, 1123, 1153
BssMI GATC 8 cut(s) 223, 451, 502, 703, 815, 856, 1009, 1105
Bst2UI CCWGG 4 cut(s) 162, 172, 1124, 1155
Bst4CI ACNGT 3 cut(s) 442, 973, 1100
Bst6I CTCTTC 1 cut(s) 1175
BstAPI GCANNNNNTGC 1 cut(s) 634
BstAUI TGTACA 1 cut(s) 1068
BstC8I GCNNGC 4 cut(s) 320, 626, 630, 804
BstDEI CTNAG 2 cut(s) 534, 1232
BstDSI CCRYGG 1 cut(s) 819
BstEII GGTNACC 1 cut(s) 733
BstHHI GCGC 1 cut(s) 793
BstKTI GATC 8 cut(s) 226, 454, 505, 706, 818, 859, 1012, 1108
BstMAI GTCTC 3 cut(s) 187, 746, 979
BstMBI GATC 8 cut(s) 223, 451, 502, 703, 815, 856, 1009, 1105
BstMWI GCNNNNNNNGC 3 cut(s) 634, 799, 1158
BstNI CCWGG 4 cut(s) 162, 172, 1124, 1155
BstNSI RCATGY 2 cut(s) 590, 632
BstPI GGTNACC 1 cut(s) 733
BstSCI CCNGG 4 cut(s) 160, 170, 1122, 1153
BstSLI GKGCMC 2 cut(s) 1134, 1154
BstV1I GCAGC 1 cut(s) 649
BstV2I GAAGAC 1 cut(s) 408
BstX2I RGATCY 2 cut(s) 223, 815
BstXI CCANNNNNNTGG 2 cut(s) 178, 923
BstYI RGATCY 2 cut(s) 223, 815
BsuRI GGCC 1 cut(s) 528
BtgI CCRYGG 1 cut(s) 819
BtsIMutI CAGTG 1 cut(s) 255
Cac8I GCNNGC 4 cut(s) 320, 626, 630, 804
CaiI CAGNNNCTG 1 cut(s) 643
CfoI GCGC 1 cut(s) 793
Cfr13I GGNCC 1 cut(s) 1169
Csp6I GTAC 3 cut(s) 438, 900, 1069
CspCI CAANNNNNGTGG 2 cut(s) 648, 683
CviQI GTAC 3 cut(s) 438, 900, 1069
DdeI CTNAG 2 cut(s) 534, 1232
DpnI GATC 8 cut(s) 225, 453, 504, 705, 817, 858, 1011, 1107
DpnII GATC 8 cut(s) 223, 451, 502, 703, 815, 856, 1009, 1105
Eam1104I CTCTTC 1 cut(s) 1175
EarI CTCTTC 1 cut(s) 1175
EciI GGCGGA 1 cut(s) 758
Eco147I AGGCCT 1 cut(s) 528
Eco31I GGTCTC 1 cut(s) 187
Eco47I GGWCC 1 cut(s) 1169
Eco91I GGTNACC 1 cut(s) 733
EcoO109I RGGNCCY 1 cut(s) 1169
EcoO65I GGTNACC 1 cut(s) 733
EcoRII CCWGG 4 cut(s) 160, 170, 1122, 1153
FaqI GGGAC 1 cut(s) 1182
FauI CCCGC 1 cut(s) 146
FauNDI CATATG 1 cut(s) 697
FbaI TGATCA 2 cut(s) 502, 1105
Fnu4HI GCNGC 2 cut(s) 6, 638
Fsp4HI GCNGC 2 cut(s) 6, 638
FspBI CTAG 5 cut(s) 381, 575, 803, 1227, 1256
GlaI GCGC 1 cut(s) 792
GluI GCNGC 2 cut(s) 6, 638
HaeIII GGCC 1 cut(s) 528
HapII CCGG 2 cut(s) 242, 273
HhaI GCGC 1 cut(s) 793
Hin6I GCGC 1 cut(s) 791
HinP1I GCGC 1 cut(s) 791
HincII GTYRAC 1 cut(s) 1096
HindII GTYRAC 1 cut(s) 1096
HindIII AAGCTT 1 cut(s) 1193
HinfI GANTC 5 cut(s) 12, 74, 211, 965, 1213
HpaI GTTAAC 1 cut(s) 1096
HpaII CCGG 2 cut(s) 242, 273
HphI GGTGA 6 cut(s) 343, 362, 461, 682, 905, 938
Hpy166II GTNNAC 3 cut(s) 823, 1096, 1132
Hpy188I TCNGA 5 cut(s) 101, 216, 645, 1105, 1247
Hpy8I GTNNAC 3 cut(s) 823, 1096, 1132
HpyAV CCTTC 3 cut(s) 65, 1072, 1203
HpyCH4III ACNGT 3 cut(s) 442, 973, 1100
HpyCH4IV ACGT 3 cut(s) 557, 611, 1111
HpyCH4V TGCA 6 cut(s) 472, 624, 632, 637, 695, 1132
HpyF10VI GCNNNNNNNGC 3 cut(s) 634, 799, 1158
HpyF3I CTNAG 2 cut(s) 534, 1232
HpySE526I ACGT 3 cut(s) 557, 611, 1111
HspAI GCGC 1 cut(s) 791
KflI GGGWCCC 1 cut(s) 1169
Kpn2I TCCGGA 1 cut(s) 272
Ksp22I TGATCA 2 cut(s) 502, 1105
KspAI GTTAAC 1 cut(s) 1096
Kzo9I GATC 8 cut(s) 223, 451, 502, 703, 815, 856, 1009, 1105
LmnI GCTCC 2 cut(s) 116, 174
Lsp1109I GCAGC 1 cut(s) 649
LweI GCATC 1 cut(s) 649
MaeI CTAG 5 cut(s) 381, 575, 803, 1227, 1256
MaeII ACGT 3 cut(s) 557, 611, 1111
MaeIII GTNAC 2 cut(s) 607, 733
MalI GATC 8 cut(s) 225, 453, 504, 705, 817, 858, 1011, 1107
MboI GATC 8 cut(s) 223, 451, 502, 703, 815, 856, 1009, 1105
MboII GAAGA 3 cut(s) 413, 1061, 1192
MfeI CAATTG 1 cut(s) 473
MflI RGATCY 2 cut(s) 223, 815
MhlI GDGCHC 2 cut(s) 1134, 1154
MluCI AATT 6 cut(s) 40, 92, 128, 133, 473, 809
MmeI TCCRAC 1 cut(s) 1014
MroI TCCGGA 1 cut(s) 272
MroXI GAANNNNTTC 1 cut(s) 1241
MseI TTAA 5 cut(s) 417, 480, 654, 1095, 1188
MslI CAYNNNNRTG 8 cut(s) 410, 645, 780, 915, 933, 948, 954, 960
MspI CCGG 2 cut(s) 242, 273
MspR9I CCNGG 4 cut(s) 162, 172, 1124, 1155
MunI CAATTG 1 cut(s) 473
MvaI CCWGG 4 cut(s) 162, 172, 1124, 1155
MwoI GCNNNNNNNGC 3 cut(s) 634, 799, 1158
NdeI CATATG 1 cut(s) 697
NdeII GATC 8 cut(s) 223, 451, 502, 703, 815, 856, 1009, 1105
NheI GCTAGC 1 cut(s) 802
NlaIV GGNNCC 6 cut(s) 271, 817, 1003, 1151, 1170, 1171
NmeAIII GCCGAG 1 cut(s) 1021
NspI RCATGY 2 cut(s) 590, 632
PaeI GCATGC 1 cut(s) 632
PceI AGGCCT 1 cut(s) 528
PciI ACATGT 1 cut(s) 586
PdmI GAANNNNTTC 1 cut(s) 1241
PfeI GAWTC 5 cut(s) 12, 74, 211, 965, 1213
PflFI GACNNNGTC 2 cut(s) 407, 956
PfoI TCCNGGA 1 cut(s) 170
PkrI GCNGC 2 cut(s) 7, 639
PpuMI RGGWCCY 1 cut(s) 1169
PscI ACATGT 1 cut(s) 586
Psp1406I AACGTT 2 cut(s) 557, 1111
Psp5II RGGWCCY 1 cut(s) 1169
Psp6I CCWGG 4 cut(s) 160, 170, 1122, 1153
PspEI GGTNACC 1 cut(s) 733
PspGI CCWGG 4 cut(s) 160, 170, 1122, 1153
PspN4I GGNNCC 6 cut(s) 271, 817, 1003, 1151, 1170, 1171
PspPI GGNCC 1 cut(s) 1169
PspPPI RGGWCCY 1 cut(s) 1169
PstNI CAGNNNCTG 1 cut(s) 643
PsuI RGATCY 2 cut(s) 223, 815
PsyI GACNNNGTC 2 cut(s) 407, 956
RsaI GTAC 3 cut(s) 439, 901, 1070
RsaNI GTAC 3 cut(s) 438, 900, 1069
RseI CAYNNNNRTG 8 cut(s) 410, 645, 780, 915, 933, 948, 954, 960
SaqAI TTAA 5 cut(s) 417, 480, 654, 1095, 1188
SatI GCNGC 2 cut(s) 6, 638
Sau3AI GATC 8 cut(s) 223, 451, 502, 703, 815, 856, 1009, 1105
Sau96I GGNCC 1 cut(s) 1169
ScrFI CCNGG 4 cut(s) 162, 172, 1124, 1155
SduI GDGCHC 2 cut(s) 1134, 1154
SfaNI GCATC 1 cut(s) 649
SinI GGWCC 1 cut(s) 1169
SmiMI CAYNNNNRTG 8 cut(s) 410, 645, 780, 915, 933, 948, 954, 960
SmlI CTYRAG 4 cut(s) 22, 35, 190, 976
SmoI CTYRAG 4 cut(s) 22, 35, 190, 976
SpeI ACTAGT 1 cut(s) 1226
SphI GCATGC 1 cut(s) 632
Sse9I AATT 6 cut(s) 40, 92, 128, 133, 473, 809
SseBI AGGCCT 1 cut(s) 528
SsiI CCGC 4 cut(s) 139, 322, 329, 743
SspMI CTAG 5 cut(s) 381, 575, 803, 1227, 1256
StuI AGGCCT 1 cut(s) 528
StyD4I CCNGG 4 cut(s) 160, 170, 1122, 1153
TaaI ACNGT 3 cut(s) 442, 973, 1100
TaiI ACGT 3 cut(s) 560, 614, 1114
TaqI TCGA 3 cut(s) 228, 861, 888
TasI AATT 6 cut(s) 40, 92, 128, 133, 473, 809
TatI WGTACW 3 cut(s) 437, 899, 1068
TfiI GAWTC 5 cut(s) 12, 74, 211, 965, 1213
Tru1I TTAA 5 cut(s) 417, 480, 654, 1095, 1188
Tru9I TTAA 5 cut(s) 417, 480, 654, 1095, 1188
TscAI CASTG 1 cut(s) 262
TseI GCWGC 2 cut(s) 5, 637
TspGWI ACGGA 1 cut(s) 808
TspRI CASTG 1 cut(s) 262
Tth111I GACNNNGTC 2 cut(s) 407, 956
VneI GTGCAC 1 cut(s) 1130
VpaK11BI GGWCC 1 cut(s) 1169
XapI RAATTY 2 cut(s) 40, 809
XceI RCATGY 2 cut(s) 590, 632
XcmI CCANNNNNNNNNTGG 1 cut(s) 1161
XmnI GAANNNNTTC 1 cut(s) 1241
XspI CTAG 5 cut(s) 381, 575, 803, 1227, 1256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.