Rroxscaffold_3G00246420

COBW domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001734
Physical Location & Seq
Reverse (-)
39523121 .. 39527799
4679 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00246420.1

Sequence Viewer

Length: 1266 bp
ATGGCTGCGAGAATCCTACTCTCAAGAACATCCCTCAAGAATTTCAATCACTTCCTTCAACCTTCTCTCCACAATTCCTCATCCTCCTCCTCTCAATTCCTCAGAAGCCTCTGCTCTCTCGCTTCCCGAGAATTCAACTCCCGCCATGCCAAGAACCCTCATCCTGGGTATTCCAGGAGCATGGTCTCTGCCTCAAGCTACAATCTTGACGAGAATCCGACATTGGGTCTCGATACCCGTGTTCCGGCGACCATTATCACCGGCTTTCTCGGCTCCGGAAAGACTACATTGCTCAATCATATACTAACATCTCAACATGGCAAGCGGATTGCGGTGATAGAAAATGAGTTTGGTGAGGTGGATATTGATGGGTCATTGGTTGCTAGTCATTCATCATCAAATGAAGACATTGTCATGGTTAATAATGGTTGCCTGTGCTGTACTGTTCGGGGTGATCTAGTAAAAATGCTCTTGCAATTGGTTAAGGAAAAGCGAGACAAGTTTGATCATATTGTTATAGAAACCACAGGCCTTGCTAAGCCATCTCCTGTTATAGAAACGTTTTGTATGGATGAGCTAGTTTCACGACATGTGAAACTGGATGGAGTTGTTACTTTGGTGGATTGCAAGCATGCAATGCAGCATCTGAATGAAGTTAAACCAAGATTTGTGGTGAATGAGGCAGTAGAACAAGTTGCATATGCTGATCGTATTATATTGAACAAGATAGATTTGGTAACCGAGGTGGAGTTAGAGACATTGACCAAGAAAATCAAGCATATCAATGGGATGGCGCAAATCAAGCTAGCTAAATTTGGATCCGTGGACATGGACTTTGTTCTGGGTGTGGGAGGATATGATCTCGAAAGGATTGATAATGAAGTTCAAGTCGATAGTTCCTGTACTTCCTCACATCACCATGAGGCTGGACATGAACATGACAACCATCACCACGACCATGTACATGATTCTACTGTCTCAAGTGTCAGTATTGTTGCCGAGGGAACCCTTGATCTTGATGAGGTGGATGACTGGTTGGAAAGACTTATTGAAGAAAAGGGGGAGGACTTGTACAGGATGAAGGGCGTTTTATGTGTTAACGGTTCTGATCAACGTTACGTTTTCCAGGGAGTGCACTCTACGTTGGATGGGTGCCCAGGAGGCACATGGGGACCCGATGAGAAGAGGATTAACAAGCTTGTGTTCATAGGAAGGAATCTGGATGAAACTAGTCTTAGGAAAGGTTTCGGAGGTTGTCTAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CobW_C cobW

Protein Analysis

421

Amino Acids

46.59

Weight (kDa)

5.83

Isoelectric Point (pI)

38.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cobW PF02492 82 - 267 4.4e-59 CobW/HypB/UreG, nucleotide-binding domain
CobW_C PF07683 326 - 420 1.6e-23 Cobalamin synthesis protein cobW C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1152
AccIII TCCGGA 1 cut(s) 275
AciI CCGC 3 cut(s) 142, 325, 332
AclI AACGTT 2 cut(s) 560, 1114
AclWI GGATC 2 cut(s) 813, 826
AcsI RAATTY 3 cut(s) 40, 131, 812
AfaI GTAC 4 cut(s) 442, 904, 963, 1073
AfiI CCNNNNNNNGG 3 cut(s) 164, 224, 244
AflIII ACRYGT 1 cut(s) 589
AgsI TTSAA 6 cut(s) 46, 59, 136, 721, 887, 1052
AhdI GACNNNNNGTC 1 cut(s) 225
AhlI ACTAGT 1 cut(s) 1229
AjnI CCWGG 4 cut(s) 163, 173, 1125, 1156
AjuI GAANNNNNNNTTGG 4 cut(s) 206, 238, 333, 365
AluBI AGCT 5 cut(s) 198, 577, 805, 809, 1198
AluI AGCT 5 cut(s) 198, 577, 805, 809, 1198
Alw21I GWGCWC 1 cut(s) 1137
Alw26I GTCTC 5 cut(s) 190, 233, 489, 749, 982
Alw44I GTGCAC 1 cut(s) 1133
AlwI GGATC 2 cut(s) 813, 826
AlwNI CAGNNNCTG 1 cut(s) 646
Ama87I CYCGRG 1 cut(s) 126
Aor13HI TCCGGA 1 cut(s) 275
AoxI GGCC 1 cut(s) 529
ApaLI GTGCAC 1 cut(s) 1133
ApeKI GCWGC 2 cut(s) 5, 640
ApoI RAATTY 3 cut(s) 40, 131, 812
ArsI GACNNNNNNTTYG 2 cut(s) 818, 850
Asp700I GAANNNNTTC 1 cut(s) 1244
AspLEI GCGC 1 cut(s) 796
AspS9I GGNCC 1 cut(s) 1172
AsuHPI GGTGA 7 cut(s) 250, 346, 365, 464, 685, 908, 941
AsuNHI GCTAGC 1 cut(s) 805
AvaI CYCGRG 1 cut(s) 126
AvaII GGWCC 1 cut(s) 1172
BaeGI GKGCMC 2 cut(s) 1137, 1157
BaeI ACNNNNGTAYC 2 cut(s) 225, 258
BamHI GGATCC 1 cut(s) 818
BanI GGYRCC 1 cut(s) 1152
BbsI GAAGAC 1 cut(s) 411
Bbv12I GWGCWC 1 cut(s) 1137
BbvI GCAGC 1 cut(s) 652
BccI CCATC 6 cut(s) 362, 550, 596, 784, 954, 1142
BciT130I CCWGG 4 cut(s) 165, 175, 1127, 1158
BclI TGATCA 2 cut(s) 505, 1108
BcoDI GTCTC 5 cut(s) 190, 233, 489, 749, 982
BcuI ACTAGT 1 cut(s) 1229
BfaI CTAG 6 cut(s) 384, 458, 578, 806, 1230, 1259
BglI GCCNNNNNGGC 1 cut(s) 1161
BisI GCNGC 2 cut(s) 6, 641
BlpI GCTNAGC 1 cut(s) 537
BlsI GCNGC 2 cut(s) 7, 642
Bme1390I CCNGG 4 cut(s) 165, 175, 1127, 1158
Bme18I GGWCC 1 cut(s) 1172
BmeRI GACNNNNNGTC 1 cut(s) 225
BmeT110I CYCGRG 1 cut(s) 126
BmgT120I GGNCC 1 cut(s) 1172
BmiI GGNNCC 6 cut(s) 274, 820, 1006, 1154, 1173, 1174
BmrFI CCNGG 4 cut(s) 165, 175, 1127, 1158
BmsI GCATC 1 cut(s) 652
BmtI GCTAGC 1 cut(s) 809
BpiI GAAGAC 1 cut(s) 411
Bpu1102I GCTNAGC 1 cut(s) 537
BpuEI CTTGAG 4 cut(s) 7, 20, 178, 964
BsaI GGTCTC 2 cut(s) 190, 233
BsaJI CCNNGG 6 cut(s) 164, 741, 822, 999, 1126, 1156
BsaWI WCCGGW 1 cut(s) 275
BsaXI ACNNNNNCTCC 4 cut(s) 51, 81, 1055, 1085
Bsc4I CCNNNNNNNGG 3 cut(s) 164, 224, 244
Bse118I RCCGGY 1 cut(s) 260
Bse1I ACTGG 2 cut(s) 603, 1037
Bse3DI GCAATG 2 cut(s) 287, 642
BseAI TCCGGA 1 cut(s) 275
BseBI CCWGG 4 cut(s) 165, 175, 1127, 1158
BseDI CCNNGG 6 cut(s) 164, 741, 822, 999, 1126, 1156
BseLI CCNNNNNNNGG 3 cut(s) 164, 224, 244
BseMI GCAATG 2 cut(s) 287, 642
BseMII CTCAG 1 cut(s) 115
BseNI ACTGG 2 cut(s) 603, 1037
BseRI GAGGAG 2 cut(s) 76, 79
BseSI GKGCMC 2 cut(s) 1137, 1157
BseXI GCAGC 1 cut(s) 652
BshFI GGCC 1 cut(s) 531
BshNI GGYRCC 1 cut(s) 1152
BsiHKAI GWGCWC 1 cut(s) 1137
BsiHKCI CYCGRG 1 cut(s) 126
BsiSI CCGG 3 cut(s) 245, 261, 276
BslFI GGGAC 1 cut(s) 1185
BslI CCNNNNNNNGG 3 cut(s) 164, 224, 244
BsmAI GTCTC 5 cut(s) 190, 233, 489, 749, 982
BsmFI GGGAC 1 cut(s) 1185
BsnI GGCC 1 cut(s) 531
Bso31I GGTCTC 2 cut(s) 190, 233
BsoBI CYCGRG 1 cut(s) 126
Bsp1286I GDGCHC 2 cut(s) 1137, 1157
Bsp13I TCCGGA 1 cut(s) 275
Bsp1407I TGTACA 2 cut(s) 961, 1071
Bsp143I GATC 7 cut(s) 454, 505, 706, 818, 859, 1012, 1108
Bsp1720I GCTNAGC 1 cut(s) 537
BspACI CCGC 3 cut(s) 142, 325, 332
BspANI GGCC 1 cut(s) 531
BspCNI CTCAG 1 cut(s) 114
BspEI TCCGGA 1 cut(s) 275
BspLI GGNNCC 6 cut(s) 274, 820, 1006, 1154, 1173, 1174
BspOI GCTAGC 1 cut(s) 809
BspPI GGATC 2 cut(s) 813, 826
BspT107I GGYRCC 1 cut(s) 1152
BspTNI GGTCTC 2 cut(s) 190, 233
BsrDI GCAATG 2 cut(s) 287, 642
BsrFI RCCGGY 1 cut(s) 260
BsrGI TGTACA 2 cut(s) 961, 1071
BsrI ACTGG 2 cut(s) 603, 1037
BssAI RCCGGY 1 cut(s) 260
BssECI CCNNGG 6 cut(s) 164, 741, 822, 999, 1126, 1156
BssMI GATC 7 cut(s) 454, 505, 706, 818, 859, 1012, 1108
Bst2UI CCWGG 4 cut(s) 165, 175, 1127, 1158
Bst4CI ACNGT 3 cut(s) 445, 976, 1103
Bst6I CTCTTC 1 cut(s) 1178
BstAPI GCANNNNNTGC 1 cut(s) 637
BstAUI TGTACA 2 cut(s) 961, 1071
BstC8I GCNNGC 4 cut(s) 323, 629, 633, 807
BstDEI CTNAG 3 cut(s) 101, 537, 1235
BstDSI CCRYGG 1 cut(s) 822
BstEII GGTNACC 1 cut(s) 736
BstHHI GCGC 1 cut(s) 796
BstKTI GATC 7 cut(s) 457, 508, 709, 821, 862, 1015, 1111
BstMAI GTCTC 5 cut(s) 190, 233, 489, 749, 982
BstMBI GATC 7 cut(s) 454, 505, 706, 818, 859, 1012, 1108
BstMWI GCNNNNNNNGC 4 cut(s) 270, 637, 802, 1161
BstNI CCWGG 4 cut(s) 165, 175, 1127, 1158
BstNSI RCATGY 2 cut(s) 593, 635
BstPI GGTNACC 1 cut(s) 736
BstSCI CCNGG 4 cut(s) 163, 173, 1125, 1156
BstSLI GKGCMC 2 cut(s) 1137, 1157
BstV1I GCAGC 1 cut(s) 652
BstV2I GAAGAC 1 cut(s) 411
BstX2I RGATCY 1 cut(s) 818
BstXI CCANNNNNNTGG 2 cut(s) 181, 926
BstYI RGATCY 1 cut(s) 818
BsuRI GGCC 1 cut(s) 531
BtgI CCRYGG 1 cut(s) 822
Cac8I GCNNGC 4 cut(s) 323, 629, 633, 807
CaiI CAGNNNCTG 1 cut(s) 646
CfoI GCGC 1 cut(s) 796
Cfr10I RCCGGY 1 cut(s) 260
Cfr13I GGNCC 1 cut(s) 1172
Csp6I GTAC 4 cut(s) 441, 903, 962, 1072
CspCI CAANNNNNGTGG 4 cut(s) 514, 549, 651, 686
CviQI GTAC 4 cut(s) 441, 903, 962, 1072
DdeI CTNAG 3 cut(s) 101, 537, 1235
DpnI GATC 7 cut(s) 456, 507, 708, 820, 861, 1014, 1110
DpnII GATC 7 cut(s) 454, 505, 706, 818, 859, 1012, 1108
DriI GACNNNNNGTC 1 cut(s) 225
Eam1104I CTCTTC 1 cut(s) 1178
Eam1105I GACNNNNNGTC 1 cut(s) 225
EarI CTCTTC 1 cut(s) 1178
Eco147I AGGCCT 1 cut(s) 531
Eco31I GGTCTC 2 cut(s) 190, 233
Eco47I GGWCC 1 cut(s) 1172
Eco88I CYCGRG 1 cut(s) 126
Eco91I GGTNACC 1 cut(s) 736
EcoO109I RGGNCCY 1 cut(s) 1172
EcoO65I GGTNACC 1 cut(s) 736
EcoRI GAATTC 1 cut(s) 131
EcoRII CCWGG 4 cut(s) 163, 173, 1125, 1156
FaqI GGGAC 1 cut(s) 1185
FauI CCCGC 1 cut(s) 149
FauNDI CATATG 1 cut(s) 700
FbaI TGATCA 2 cut(s) 505, 1108
Fnu4HI GCNGC 2 cut(s) 6, 641
Fsp4HI GCNGC 2 cut(s) 6, 641
FspBI CTAG 6 cut(s) 384, 458, 578, 806, 1230, 1259
GlaI GCGC 1 cut(s) 795
GluI GCNGC 2 cut(s) 6, 641
HaeIII GGCC 1 cut(s) 531
HapII CCGG 3 cut(s) 245, 261, 276
HhaI GCGC 1 cut(s) 796
Hin6I GCGC 1 cut(s) 794
HinP1I GCGC 1 cut(s) 794
HincII GTYRAC 1 cut(s) 1099
HindII GTYRAC 1 cut(s) 1099
HindIII AAGCTT 1 cut(s) 1196
HinfI GANTC 4 cut(s) 12, 214, 968, 1216
HpaI GTTAAC 1 cut(s) 1099
HpaII CCGG 3 cut(s) 245, 261, 276
HphI GGTGA 7 cut(s) 250, 346, 365, 464, 685, 908, 941
Hpy166II GTNNAC 3 cut(s) 826, 1099, 1135
Hpy188I TCNGA 5 cut(s) 104, 219, 648, 1108, 1250
Hpy8I GTNNAC 3 cut(s) 826, 1099, 1135
HpyAV CCTTC 4 cut(s) 65, 72, 1075, 1206
HpyCH4III ACNGT 3 cut(s) 445, 976, 1103
HpyCH4IV ACGT 4 cut(s) 560, 1114, 1119, 1142
HpyCH4V TGCA 6 cut(s) 475, 627, 635, 640, 698, 1135
HpyF10VI GCNNNNNNNGC 4 cut(s) 270, 637, 802, 1161
HpyF3I CTNAG 3 cut(s) 101, 537, 1235
HpySE526I ACGT 4 cut(s) 560, 1114, 1119, 1142
HspAI GCGC 1 cut(s) 794
KflI GGGWCCC 1 cut(s) 1172
Kpn2I TCCGGA 1 cut(s) 275
Ksp22I TGATCA 2 cut(s) 505, 1108
KspAI GTTAAC 1 cut(s) 1099
Kzo9I GATC 7 cut(s) 454, 505, 706, 818, 859, 1012, 1108
LmnI GCTCC 2 cut(s) 177, 278
Lsp1109I GCAGC 1 cut(s) 652
LweI GCATC 1 cut(s) 652
MaeI CTAG 6 cut(s) 384, 458, 578, 806, 1230, 1259
MaeII ACGT 4 cut(s) 560, 1114, 1119, 1142
MaeIII GTNAC 3 cut(s) 610, 736, 1115
MalI GATC 7 cut(s) 456, 507, 708, 820, 861, 1014, 1110
MboI GATC 7 cut(s) 454, 505, 706, 818, 859, 1012, 1108
MboII GAAGA 3 cut(s) 416, 1064, 1195
MfeI CAATTG 1 cut(s) 476
MflI RGATCY 1 cut(s) 818
MhlI GDGCHC 2 cut(s) 1137, 1157
MluCI AATT 6 cut(s) 40, 73, 95, 131, 476, 812
MmeI TCCRAC 3 cut(s) 242, 1017, 1125
MroI TCCGGA 1 cut(s) 275
MroXI GAANNNNTTC 1 cut(s) 1244
MseI TTAA 5 cut(s) 420, 483, 657, 1098, 1191
MslI CAYNNNNRTG 7 cut(s) 413, 648, 783, 918, 936, 957, 963
MspI CCGG 3 cut(s) 245, 261, 276
MspR9I CCNGG 4 cut(s) 165, 175, 1127, 1158
MunI CAATTG 1 cut(s) 476
MvaI CCWGG 4 cut(s) 165, 175, 1127, 1158
MwoI GCNNNNNNNGC 4 cut(s) 270, 637, 802, 1161
NdeI CATATG 1 cut(s) 700
NdeII GATC 7 cut(s) 454, 505, 706, 818, 859, 1012, 1108
NheI GCTAGC 1 cut(s) 805
NlaIV GGNNCC 6 cut(s) 274, 820, 1006, 1154, 1173, 1174
NmeAIII GCCGAG 2 cut(s) 249, 1024
NspI RCATGY 2 cut(s) 593, 635
PaeI GCATGC 1 cut(s) 635
PceI AGGCCT 1 cut(s) 531
PciI ACATGT 1 cut(s) 589
PdmI GAANNNNTTC 1 cut(s) 1244
PfeI GAWTC 4 cut(s) 12, 214, 968, 1216
PflFI GACNNNGTC 1 cut(s) 410
PfoI TCCNGGA 1 cut(s) 173
PkrI GCNGC 2 cut(s) 7, 642
PpuMI RGGWCCY 1 cut(s) 1172
PscI ACATGT 1 cut(s) 589
Psp1406I AACGTT 2 cut(s) 560, 1114
Psp5II RGGWCCY 1 cut(s) 1172
Psp6I CCWGG 4 cut(s) 163, 173, 1125, 1156
PspEI GGTNACC 1 cut(s) 736
PspGI CCWGG 4 cut(s) 163, 173, 1125, 1156
PspN4I GGNNCC 6 cut(s) 274, 820, 1006, 1154, 1173, 1174
PspPI GGNCC 1 cut(s) 1172
PspPPI RGGWCCY 1 cut(s) 1172
PstNI CAGNNNCTG 1 cut(s) 646
PsuI RGATCY 1 cut(s) 818
PsyI GACNNNGTC 1 cut(s) 410
RsaI GTAC 4 cut(s) 442, 904, 963, 1073
RsaNI GTAC 4 cut(s) 441, 903, 962, 1072
RseI CAYNNNNRTG 7 cut(s) 413, 648, 783, 918, 936, 957, 963
SaqAI TTAA 5 cut(s) 420, 483, 657, 1098, 1191
SatI GCNGC 2 cut(s) 6, 641
Sau3AI GATC 7 cut(s) 454, 505, 706, 818, 859, 1012, 1108
Sau96I GGNCC 1 cut(s) 1172
ScrFI CCNGG 4 cut(s) 165, 175, 1127, 1158
SduI GDGCHC 2 cut(s) 1137, 1157
SfaNI GCATC 1 cut(s) 652
SinI GGWCC 1 cut(s) 1172
SmiMI CAYNNNNRTG 7 cut(s) 413, 648, 783, 918, 936, 957, 963
SmlI CTYRAG 4 cut(s) 22, 35, 193, 979
SmoI CTYRAG 4 cut(s) 22, 35, 193, 979
SpeI ACTAGT 1 cut(s) 1229
SphI GCATGC 1 cut(s) 635
Sse9I AATT 6 cut(s) 40, 73, 95, 131, 476, 812
SseBI AGGCCT 1 cut(s) 531
SsiI CCGC 3 cut(s) 142, 325, 332
SspMI CTAG 6 cut(s) 384, 458, 578, 806, 1230, 1259
StuI AGGCCT 1 cut(s) 531
StyD4I CCNGG 4 cut(s) 163, 173, 1125, 1156
TaaI ACNGT 3 cut(s) 445, 976, 1103
TaiI ACGT 4 cut(s) 563, 1117, 1122, 1145
TaqI TCGA 3 cut(s) 231, 864, 891
TasI AATT 6 cut(s) 40, 73, 95, 131, 476, 812
TatI WGTACW 4 cut(s) 440, 902, 961, 1071
TfiI GAWTC 4 cut(s) 12, 214, 968, 1216
Tru1I TTAA 5 cut(s) 420, 483, 657, 1098, 1191
Tru9I TTAA 5 cut(s) 420, 483, 657, 1098, 1191
TseI GCWGC 2 cut(s) 5, 640
TspDTI ATGAA 8 cut(s) 381, 417, 666, 894, 948, 1094, 1195, 1239
TspGWI ACGGA 1 cut(s) 811
Tth111I GACNNNGTC 1 cut(s) 410
VneI GTGCAC 1 cut(s) 1133
VpaK11BI GGWCC 1 cut(s) 1172
XapI RAATTY 3 cut(s) 40, 131, 812
XceI RCATGY 2 cut(s) 593, 635
XcmI CCANNNNNNNNNTGG 1 cut(s) 1164
XmnI GAANNNNTTC 1 cut(s) 1244
XspI CTAG 6 cut(s) 384, 458, 578, 806, 1230, 1259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.