Rh7AG269300

COBW domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0001734
Physical Location & Seq
Forward (+)
28072055 .. 28076449
4395 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG269300.1

Sequence Viewer

Length: 1263 bp
ATGGCTGCAAGAATCCTACTCTCGAGAACATCCCTCAAGAATTTCAATCACTTCCTTCATCCTCCTATCCACGATTCCTCATCCTCCTCTCAATTCCTCCGAAGCCTATGCTCCCTCGTTTCCCGACAATTCAATTCCCGCCATGCCAAGAACCCTCATCCTGGGTATTCCAGGAGCATGGTCTCTGCCTCAAGCTACAATCTTGACGAGAATCCGAGCTTGGGTCTCGATACCCGTGTTCCGGCGACCATTATCACCGGCTTTCTCGGTTCCGGAAAGACTACATTGCTCAATCATATACTAACATCTCAACATGGCAAGCGGATTGCGGTCATAGAAAATGAGTTTGGTGAGGTGGATATTGATGGGTCATTGGTTGCTAGTCATTCATCATCAAATGAAGACATTGTCATGGTTAATAATGGTTGCCTATGCTGTACTGTTCGGGGTGATCTAGTAAAAATGCTCTTGCAATTGGTTAAGGAAAAGCGAGACAAGTTTGATCATATTGTCATAGAAACCACAGGCCTCGCTAAGCCATCACCTGTTATAGAAACGTTTTGTATGGATGAGCTAGTTTCACAACATGTGAAACTGGATGGAGTTGTTACTTTGGTGGATTGCAAGCATGCAATGCAGCATCTGAATGAAGTTAAACCAAGATTTGTGGTGAATGAGGCAGTAGAACAAGTTGCATATGCTGATCGTATTATATTGAACAAGATAGATTTGGTAACCGAGGTGGAGTTAGAGATATTGACCAAGAAAATCAAGCATATCAATGGGATGGCGCAAATCAAGCTAGCTAAATTTGGATCCGTGGACATGGACTTTGTTCTGGGTGTGGGAGGATATGATCTCGAAAGGATTGATAATGAAGTTCAAGTCGATAGTTCCTGTACTTCCTCACATCACCATGAAGCTGGACATGAACATGACAACCATCACCACGACCATGTACATGATTCTACTGTCTCAAGTGTCAGTATTGTTGCCGAGGGAACCCTTGATCTTGATGAGGTGGATGACTGGTTGGAAAGACTTATTGAAGAAAAGGGGGAGGACTTGTACAGGATGAAGGGCGTTTTATGTGTTAACGGTTCTGATCAACGTTATGTTTTCCAGGGAGTGCACTCTACATTGGATGGGTGCCCAGGAGGCACATGGGGACCCGATGAGAAGAGGATTAACAAGCTTGTGTTCATAGGAAGGAATCTGGATGAAACTAGTCTTAGGAAAGGTTTCAGAGGTTGTCTAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CobW_C cobW

Protein Analysis

420

Amino Acids

46.62

Weight (kDa)

5.86

Isoelectric Point (pI)

38.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cobW PF02492 81 - 266 7.3e-59 CobW/HypB/UreG, nucleotide-binding domain
CobW_C PF07683 325 - 419 6.3e-24 Cobalamin synthesis protein cobW C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1149
AccIII TCCGGA 1 cut(s) 272
AciI CCGC 3 cut(s) 139, 322, 329
AclI AACGTT 2 cut(s) 557, 1111
AclWI GGATC 2 cut(s) 810, 823
AcsI RAATTY 2 cut(s) 40, 809
AfaI GTAC 4 cut(s) 439, 901, 960, 1070
AfiI CCNNNNNNNGG 3 cut(s) 161, 221, 241
AflIII ACRYGT 1 cut(s) 586
AgsI TTSAA 5 cut(s) 46, 133, 718, 884, 1049
AhlI ACTAGT 1 cut(s) 1226
AjnI CCWGG 4 cut(s) 160, 170, 1122, 1153
AjuI GAANNNNNNNTTGG 4 cut(s) 203, 235, 330, 362
AluBI AGCT 7 cut(s) 195, 219, 574, 802, 806, 923, 1195
AluI AGCT 7 cut(s) 195, 219, 574, 802, 806, 923, 1195
Alw21I GWGCWC 1 cut(s) 1134
Alw26I GTCTC 4 cut(s) 187, 230, 486, 979
Alw44I GTGCAC 1 cut(s) 1130
AlwI GGATC 2 cut(s) 810, 823
AlwNI CAGNNNCTG 1 cut(s) 643
Ama87I CYCGRG 1 cut(s) 22
Aor13HI TCCGGA 1 cut(s) 272
AoxI GGCC 1 cut(s) 526
ApaLI GTGCAC 1 cut(s) 1130
ApeKI GCWGC 2 cut(s) 5, 637
ApoI RAATTY 2 cut(s) 40, 809
ArsI GACNNNNNNTTYG 2 cut(s) 815, 847
Asp700I GAANNNNTTC 1 cut(s) 1241
AspLEI GCGC 1 cut(s) 793
AspS9I GGNCC 1 cut(s) 1169
AsuHPI GGTGA 7 cut(s) 247, 362, 461, 534, 682, 905, 938
AsuNHI GCTAGC 1 cut(s) 802
AvaI CYCGRG 1 cut(s) 22
AvaII GGWCC 1 cut(s) 1169
BaeGI GKGCMC 2 cut(s) 1134, 1154
BaeI ACNNNNGTAYC 2 cut(s) 222, 255
BamHI GGATCC 1 cut(s) 815
BanI GGYRCC 1 cut(s) 1149
BbsI GAAGAC 1 cut(s) 408
Bbv12I GWGCWC 1 cut(s) 1134
BbvI GCAGC 1 cut(s) 649
BccI CCATC 6 cut(s) 359, 547, 593, 781, 951, 1139
BcgI CGANNNNNNTGC 2 cut(s) 90, 124
BciT130I CCWGG 4 cut(s) 162, 172, 1124, 1155
BclI TGATCA 2 cut(s) 502, 1105
BcoDI GTCTC 4 cut(s) 187, 230, 486, 979
BcuI ACTAGT 1 cut(s) 1226
BfaI CTAG 6 cut(s) 381, 455, 575, 803, 1227, 1256
BglI GCCNNNNNGGC 1 cut(s) 1158
BisI GCNGC 2 cut(s) 6, 638
BlpI GCTNAGC 1 cut(s) 534
BlsI GCNGC 2 cut(s) 7, 639
Bme1390I CCNGG 4 cut(s) 162, 172, 1124, 1155
Bme18I GGWCC 1 cut(s) 1169
BmeT110I CYCGRG 1 cut(s) 22
BmgT120I GGNCC 1 cut(s) 1169
BmiI GGNNCC 6 cut(s) 271, 817, 1003, 1151, 1170, 1171
BmrFI CCNGG 4 cut(s) 162, 172, 1124, 1155
BmsI GCATC 1 cut(s) 649
BmtI GCTAGC 1 cut(s) 806
BpiI GAAGAC 1 cut(s) 408
Bpu1102I GCTNAGC 1 cut(s) 534
BpuEI CTTGAG 3 cut(s) 20, 175, 961
BsaI GGTCTC 2 cut(s) 187, 230
BsaJI CCNNGG 6 cut(s) 161, 738, 819, 996, 1123, 1153
BsaWI WCCGGW 1 cut(s) 272
BsaXI ACNNNNNCTCC 2 cut(s) 1052, 1082
Bsc4I CCNNNNNNNGG 3 cut(s) 161, 221, 241
Bse118I RCCGGY 1 cut(s) 257
Bse1I ACTGG 2 cut(s) 600, 1034
Bse3DI GCAATG 2 cut(s) 284, 639
BseAI TCCGGA 1 cut(s) 272
BseBI CCWGG 4 cut(s) 162, 172, 1124, 1155
BseDI CCNNGG 6 cut(s) 161, 738, 819, 996, 1123, 1153
BseLI CCNNNNNNNGG 3 cut(s) 161, 221, 241
BseMI GCAATG 2 cut(s) 284, 639
BseNI ACTGG 2 cut(s) 600, 1034
BseRI GAGGAG 1 cut(s) 76
BseSI GKGCMC 2 cut(s) 1134, 1154
BseXI GCAGC 1 cut(s) 649
BshFI GGCC 1 cut(s) 528
BshNI GGYRCC 1 cut(s) 1149
BsiHKAI GWGCWC 1 cut(s) 1134
BsiHKCI CYCGRG 1 cut(s) 22
BsiSI CCGG 3 cut(s) 242, 258, 273
BslFI GGGAC 1 cut(s) 1182
BslI CCNNNNNNNGG 3 cut(s) 161, 221, 241
BsmAI GTCTC 4 cut(s) 187, 230, 486, 979
BsmFI GGGAC 1 cut(s) 1182
BsnI GGCC 1 cut(s) 528
Bso31I GGTCTC 2 cut(s) 187, 230
BsoBI CYCGRG 1 cut(s) 22
Bsp1286I GDGCHC 2 cut(s) 1134, 1154
Bsp13I TCCGGA 1 cut(s) 272
Bsp1407I TGTACA 2 cut(s) 958, 1068
Bsp143I GATC 7 cut(s) 451, 502, 703, 815, 856, 1009, 1105
Bsp1720I GCTNAGC 1 cut(s) 534
BspACI CCGC 3 cut(s) 139, 322, 329
BspANI GGCC 1 cut(s) 528
BspEI TCCGGA 1 cut(s) 272
BspLI GGNNCC 6 cut(s) 271, 817, 1003, 1151, 1170, 1171
BspOI GCTAGC 1 cut(s) 806
BspPI GGATC 2 cut(s) 810, 823
BspT107I GGYRCC 1 cut(s) 1149
BspTNI GGTCTC 2 cut(s) 187, 230
BsrDI GCAATG 2 cut(s) 284, 639
BsrFI RCCGGY 1 cut(s) 257
BsrGI TGTACA 2 cut(s) 958, 1068
BsrI ACTGG 2 cut(s) 600, 1034
BssAI RCCGGY 1 cut(s) 257
BssECI CCNNGG 6 cut(s) 161, 738, 819, 996, 1123, 1153
BssMI GATC 7 cut(s) 451, 502, 703, 815, 856, 1009, 1105
Bst2UI CCWGG 4 cut(s) 162, 172, 1124, 1155
Bst4CI ACNGT 3 cut(s) 442, 973, 1100
Bst6I CTCTTC 1 cut(s) 1175
BstAPI GCANNNNNTGC 1 cut(s) 634
BstAUI TGTACA 2 cut(s) 958, 1068
BstC8I GCNNGC 4 cut(s) 320, 626, 630, 804
BstDEI CTNAG 2 cut(s) 534, 1232
BstDSI CCRYGG 1 cut(s) 819
BstEII GGTNACC 1 cut(s) 733
BstHHI GCGC 1 cut(s) 793
BstKTI GATC 7 cut(s) 454, 505, 706, 818, 859, 1012, 1108
BstMAI GTCTC 4 cut(s) 187, 230, 486, 979
BstMBI GATC 7 cut(s) 451, 502, 703, 815, 856, 1009, 1105
BstMWI GCNNNNNNNGC 3 cut(s) 634, 799, 1158
BstNI CCWGG 4 cut(s) 162, 172, 1124, 1155
BstNSI RCATGY 2 cut(s) 590, 632
BstPI GGTNACC 1 cut(s) 733
BstSCI CCNGG 4 cut(s) 160, 170, 1122, 1153
BstSLI GKGCMC 2 cut(s) 1134, 1154
BstV1I GCAGC 1 cut(s) 649
BstV2I GAAGAC 1 cut(s) 408
BstX2I RGATCY 1 cut(s) 815
BstXI CCANNNNNNTGG 2 cut(s) 178, 923
BstYI RGATCY 1 cut(s) 815
BsuRI GGCC 1 cut(s) 528
BtgI CCRYGG 1 cut(s) 819
Cac8I GCNNGC 4 cut(s) 320, 626, 630, 804
CaiI CAGNNNCTG 1 cut(s) 643
CfoI GCGC 1 cut(s) 793
Cfr10I RCCGGY 1 cut(s) 257
Cfr13I GGNCC 1 cut(s) 1169
Csp6I GTAC 4 cut(s) 438, 900, 959, 1069
CspCI CAANNNNNGTGG 2 cut(s) 648, 683
CviQI GTAC 4 cut(s) 438, 900, 959, 1069
DdeI CTNAG 2 cut(s) 534, 1232
DpnI GATC 7 cut(s) 453, 504, 705, 817, 858, 1011, 1107
DpnII GATC 7 cut(s) 451, 502, 703, 815, 856, 1009, 1105
Eam1104I CTCTTC 1 cut(s) 1175
EarI CTCTTC 1 cut(s) 1175
Eco147I AGGCCT 1 cut(s) 528
Eco31I GGTCTC 2 cut(s) 187, 230
Eco47I GGWCC 1 cut(s) 1169
Eco88I CYCGRG 1 cut(s) 22
Eco91I GGTNACC 1 cut(s) 733
EcoO109I RGGNCCY 1 cut(s) 1169
EcoO65I GGTNACC 1 cut(s) 733
EcoRII CCWGG 4 cut(s) 160, 170, 1122, 1153
FaqI GGGAC 1 cut(s) 1182
FauI CCCGC 1 cut(s) 146
FauNDI CATATG 1 cut(s) 697
FbaI TGATCA 2 cut(s) 502, 1105
Fnu4HI GCNGC 2 cut(s) 6, 638
Fsp4HI GCNGC 2 cut(s) 6, 638
FspBI CTAG 6 cut(s) 381, 455, 575, 803, 1227, 1256
GlaI GCGC 1 cut(s) 792
GluI GCNGC 2 cut(s) 6, 638
HaeIII GGCC 1 cut(s) 528
HapII CCGG 3 cut(s) 242, 258, 273
HhaI GCGC 1 cut(s) 793
Hin6I GCGC 1 cut(s) 791
HinP1I GCGC 1 cut(s) 791
HincII GTYRAC 1 cut(s) 1096
HindII GTYRAC 1 cut(s) 1096
HindIII AAGCTT 1 cut(s) 1193
HinfI GANTC 5 cut(s) 12, 74, 211, 965, 1213
HpaI GTTAAC 1 cut(s) 1096
HpaII CCGG 3 cut(s) 242, 258, 273
HphI GGTGA 7 cut(s) 247, 362, 461, 534, 682, 905, 938
Hpy166II GTNNAC 3 cut(s) 823, 1096, 1132
Hpy188I TCNGA 5 cut(s) 101, 216, 645, 1105, 1247
Hpy8I GTNNAC 3 cut(s) 823, 1096, 1132
HpyAV CCTTC 3 cut(s) 65, 1072, 1203
HpyCH4III ACNGT 3 cut(s) 442, 973, 1100
HpyCH4IV ACGT 2 cut(s) 557, 1111
HpyCH4V TGCA 7 cut(s) 8, 472, 624, 632, 637, 695, 1132
HpyF10VI GCNNNNNNNGC 3 cut(s) 634, 799, 1158
HpyF3I CTNAG 2 cut(s) 534, 1232
HpySE526I ACGT 2 cut(s) 557, 1111
HspAI GCGC 1 cut(s) 791
KflI GGGWCCC 1 cut(s) 1169
Kpn2I TCCGGA 1 cut(s) 272
Ksp22I TGATCA 2 cut(s) 502, 1105
KspAI GTTAAC 1 cut(s) 1096
Kzo9I GATC 7 cut(s) 451, 502, 703, 815, 856, 1009, 1105
LmnI GCTCC 2 cut(s) 116, 174
Lsp1109I GCAGC 1 cut(s) 649
LweI GCATC 1 cut(s) 649
MaeI CTAG 6 cut(s) 381, 455, 575, 803, 1227, 1256
MaeII ACGT 2 cut(s) 557, 1111
MaeIII GTNAC 2 cut(s) 607, 733
MalI GATC 7 cut(s) 453, 504, 705, 817, 858, 1011, 1107
MboI GATC 7 cut(s) 451, 502, 703, 815, 856, 1009, 1105
MboII GAAGA 3 cut(s) 413, 1061, 1192
MfeI CAATTG 1 cut(s) 473
MflI RGATCY 1 cut(s) 815
MhlI GDGCHC 2 cut(s) 1134, 1154
MluCI AATT 6 cut(s) 40, 92, 128, 133, 473, 809
MmeI TCCRAC 1 cut(s) 1014
MroI TCCGGA 1 cut(s) 272
MroXI GAANNNNTTC 1 cut(s) 1241
MseI TTAA 5 cut(s) 417, 480, 654, 1095, 1188
MslI CAYNNNNRTG 7 cut(s) 410, 645, 780, 915, 933, 954, 960
MspI CCGG 3 cut(s) 242, 258, 273
MspR9I CCNGG 4 cut(s) 162, 172, 1124, 1155
MunI CAATTG 1 cut(s) 473
MvaI CCWGG 4 cut(s) 162, 172, 1124, 1155
MwoI GCNNNNNNNGC 3 cut(s) 634, 799, 1158
NdeI CATATG 1 cut(s) 697
NdeII GATC 7 cut(s) 451, 502, 703, 815, 856, 1009, 1105
NheI GCTAGC 1 cut(s) 802
NlaIV GGNNCC 6 cut(s) 271, 817, 1003, 1151, 1170, 1171
NmeAIII GCCGAG 1 cut(s) 1021
NspI RCATGY 2 cut(s) 590, 632
PaeI GCATGC 1 cut(s) 632
PaeR7I CTCGAG 1 cut(s) 22
PceI AGGCCT 1 cut(s) 528
PciI ACATGT 1 cut(s) 586
PdmI GAANNNNTTC 1 cut(s) 1241
PfeI GAWTC 5 cut(s) 12, 74, 211, 965, 1213
PflFI GACNNNGTC 1 cut(s) 407
PfoI TCCNGGA 1 cut(s) 170
PkrI GCNGC 2 cut(s) 7, 639
PpuMI RGGWCCY 1 cut(s) 1169
PscI ACATGT 1 cut(s) 586
Psp1406I AACGTT 2 cut(s) 557, 1111
Psp5II RGGWCCY 1 cut(s) 1169
Psp6I CCWGG 4 cut(s) 160, 170, 1122, 1153
PspEI GGTNACC 1 cut(s) 733
PspGI CCWGG 4 cut(s) 160, 170, 1122, 1153
PspN4I GGNNCC 6 cut(s) 271, 817, 1003, 1151, 1170, 1171
PspPI GGNCC 1 cut(s) 1169
PspPPI RGGWCCY 1 cut(s) 1169
PstNI CAGNNNCTG 1 cut(s) 643
PsuI RGATCY 1 cut(s) 815
PsyI GACNNNGTC 1 cut(s) 407
RsaI GTAC 4 cut(s) 439, 901, 960, 1070
RsaNI GTAC 4 cut(s) 438, 900, 959, 1069
RseI CAYNNNNRTG 7 cut(s) 410, 645, 780, 915, 933, 954, 960
SaqAI TTAA 5 cut(s) 417, 480, 654, 1095, 1188
SatI GCNGC 2 cut(s) 6, 638
Sau3AI GATC 7 cut(s) 451, 502, 703, 815, 856, 1009, 1105
Sau96I GGNCC 1 cut(s) 1169
ScrFI CCNGG 4 cut(s) 162, 172, 1124, 1155
SduI GDGCHC 2 cut(s) 1134, 1154
SfaNI GCATC 1 cut(s) 649
Sfr274I CTCGAG 1 cut(s) 22
SinI GGWCC 1 cut(s) 1169
SlaI CTCGAG 1 cut(s) 22
SmiMI CAYNNNNRTG 7 cut(s) 410, 645, 780, 915, 933, 954, 960
SmlI CTYRAG 4 cut(s) 22, 35, 190, 976
SmoI CTYRAG 4 cut(s) 22, 35, 190, 976
SpeI ACTAGT 1 cut(s) 1226
SphI GCATGC 1 cut(s) 632
Sse9I AATT 6 cut(s) 40, 92, 128, 133, 473, 809
SseBI AGGCCT 1 cut(s) 528
SsiI CCGC 3 cut(s) 139, 322, 329
SspMI CTAG 6 cut(s) 381, 455, 575, 803, 1227, 1256
StuI AGGCCT 1 cut(s) 528
StyD4I CCNGG 4 cut(s) 160, 170, 1122, 1153
TaaI ACNGT 3 cut(s) 442, 973, 1100
TaiI ACGT 2 cut(s) 560, 1114
TaqI TCGA 4 cut(s) 23, 228, 861, 888
TasI AATT 6 cut(s) 40, 92, 128, 133, 473, 809
TatI WGTACW 4 cut(s) 437, 899, 958, 1068
TfiI GAWTC 5 cut(s) 12, 74, 211, 965, 1213
Tru1I TTAA 5 cut(s) 417, 480, 654, 1095, 1188
Tru9I TTAA 5 cut(s) 417, 480, 654, 1095, 1188
TseI GCWGC 2 cut(s) 5, 637
TspGWI ACGGA 1 cut(s) 808
Tth111I GACNNNGTC 1 cut(s) 407
VneI GTGCAC 1 cut(s) 1130
VpaK11BI GGWCC 1 cut(s) 1169
XapI RAATTY 2 cut(s) 40, 809
XceI RCATGY 2 cut(s) 590, 632
XcmI CCANNNNNNNNNTGG 1 cut(s) 1161
XhoI CTCGAG 1 cut(s) 22
XmnI GAANNNNTTC 1 cut(s) 1241
XspI CTAG 6 cut(s) 381, 455, 575, 803, 1227, 1256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.