Rw7G023400

COBW domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0001734
Physical Location & Seq
Forward (+)
28052643 .. 28057583
4941 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G023400.1

Sequence Viewer

Length: 1155 bp
ATGCTCCCTCGTTTCCCGACAATTCAATTCCCGCCATGCCAAGAACCCTCATCCTGGTATTCCAGGAGCATGGTCTCTGCCTCAAGCTACAATCTTGACGAGAATCCGAGCTTGGGTCTCGATACCCGTGTTCCGGCGACCATTATCACCGGCTTTCTCGGTTCCGGAAAGACTACATTGCTCAATCATATACTAACATCTCAACATGGAAAGCGGATTGCGGTCATAGAAAATGAGTTTGGTGAGGTGGATATTGATGGGTCATTGGTTGCTAGTCATTCATCATCAAATGAAGACATTGTCATGGTTAATAATGGTTGCCTATGCTGTACTGTTCGGGGTGACCTAGTAAAAATGCTCTTGCAATTGGTTAAGGAAAAGCGAGACAAGTTTGATCATATTGTCATAGAAACCACAGGCCTCGCTAAGCCATCACCTGTTATAGAAACGTTTTGTATGGATGAGCTAGTTTCACGACATGTGAAACTGGATGGAGTTGTTACTTTGGTGGATTGCAAGCATGCAATGCAGCATCTGAATGAAGTTAAACCAAGATTTGTGGTGAATGAGGCAGTAGAACAAGTTGCATATGCTGATCGTATTATATTGAACAAGATAGATTTGGTAACTGAGGCGGAGTTAGAGACATTGACCAAGAAAATCAAGCATATCAATGGGATGGCGCAAATCAAGCTAGCTAAATTTGGATCCGTGGACATGGACTTTGTTCTGGGTGTGGGAGGATATGATCTCGAAAGGATTGATAATGAAGTTCAAGTCGATAGTTCCTGTACTTCCTCACATCACCATGAAGCTGGACATGAACATGACAACCATCACCACGACCATGTACATGATTCTACTGTCTCAAGTGTCAGTATTGTTGCCGAGGGAACCCTTGATCTTGATGAGGTGGATGACTGGTTGGAAAGACTTATTGAAGAAAAGGGGGAGGACTTGTACAGGATGAAGGGCGTTTTATGTGTTAACGGTTCTGATCAACGTTATGTTTTCCAGGGAGTGCACTCTACGTTGGATGGGTGCCCAGGGGGCACATGGGGACCCGATGAGAAGAGGATTAACAAGCTTGTGTTCATAGGAAGGAATCTGGATGAAACTAGTCTTAGGAAAGGTTTCAGAGGTTGTCTAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CobW_C cobW

Protein Analysis

384

Amino Acids

42.64

Weight (kDa)

5.35

Isoelectric Point (pI)

36.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cobW PF02492 45 - 230 2.5e-59 CobW/HypB/UreG, nucleotide-binding domain
CobW_C PF07683 289 - 383 5.4e-24 Cobalamin synthesis protein cobW C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1041
AccIII TCCGGA 1 cut(s) 164
AciI CCGC 4 cut(s) 32, 214, 221, 635
AclI AACGTT 2 cut(s) 449, 1003
AclWI GGATC 2 cut(s) 702, 715
AcsI RAATTY 1 cut(s) 701
AfaI GTAC 4 cut(s) 331, 793, 852, 962
AfiI CCNNNNNNNGG 3 cut(s) 54, 113, 133
AflIII ACRYGT 1 cut(s) 478
AgsI TTSAA 4 cut(s) 26, 610, 776, 941
AhlI ACTAGT 1 cut(s) 1118
AjnI CCWGG 4 cut(s) 53, 62, 1014, 1045
AjuI GAANNNNNNNTTGG 4 cut(s) 95, 127, 222, 254
AluBI AGCT 7 cut(s) 87, 111, 466, 694, 698, 815, 1087
AluI AGCT 7 cut(s) 87, 111, 466, 694, 698, 815, 1087
Alw21I GWGCWC 1 cut(s) 1026
Alw26I GTCTC 5 cut(s) 79, 122, 378, 638, 871
Alw44I GTGCAC 1 cut(s) 1022
AlwI GGATC 2 cut(s) 702, 715
AlwNI CAGNNNCTG 1 cut(s) 535
Aor13HI TCCGGA 1 cut(s) 164
AoxI GGCC 1 cut(s) 418
ApaLI GTGCAC 1 cut(s) 1022
ApeKI GCWGC 1 cut(s) 529
ApoI RAATTY 1 cut(s) 701
ArsI GACNNNNNNTTYG 2 cut(s) 707, 739
Asp700I GAANNNNTTC 1 cut(s) 1133
AspLEI GCGC 1 cut(s) 685
AspS9I GGNCC 1 cut(s) 1061
AsuHPI GGTGA 7 cut(s) 139, 254, 353, 426, 574, 797, 830
AsuNHI GCTAGC 1 cut(s) 694
AvaII GGWCC 1 cut(s) 1061
BaeGI GKGCMC 3 cut(s) 1026, 1046, 1055
BaeI ACNNNNGTAYC 2 cut(s) 114, 147
BamHI GGATCC 1 cut(s) 707
BanI GGYRCC 1 cut(s) 1041
BbsI GAAGAC 1 cut(s) 300
Bbv12I GWGCWC 1 cut(s) 1026
BbvI GCAGC 1 cut(s) 541
BccI CCATC 6 cut(s) 251, 439, 485, 673, 843, 1031
BciT130I CCWGG 4 cut(s) 55, 64, 1016, 1047
BclI TGATCA 2 cut(s) 394, 997
BcoDI GTCTC 5 cut(s) 79, 122, 378, 638, 871
BcuI ACTAGT 1 cut(s) 1118
BfaI CTAG 6 cut(s) 273, 347, 467, 695, 1119, 1148
BglI GCCNNNNNGGC 1 cut(s) 1050
BisI GCNGC 1 cut(s) 530
BlpI GCTNAGC 1 cut(s) 426
BlsI GCNGC 1 cut(s) 531
Bme1390I CCNGG 4 cut(s) 55, 64, 1016, 1047
Bme18I GGWCC 1 cut(s) 1061
BmgT120I GGNCC 1 cut(s) 1061
BmiI GGNNCC 6 cut(s) 163, 709, 895, 1043, 1062, 1063
BmrFI CCNGG 4 cut(s) 55, 64, 1016, 1047
BmsI GCATC 1 cut(s) 541
BmtI GCTAGC 1 cut(s) 698
BpiI GAAGAC 1 cut(s) 300
Bpu1102I GCTNAGC 1 cut(s) 426
BpuEI CTTGAG 2 cut(s) 67, 853
BsaI GGTCTC 2 cut(s) 79, 122
BsaJI CCNNGG 5 cut(s) 711, 888, 1015, 1045, 1046
BsaWI WCCGGW 1 cut(s) 164
BsaXI ACNNNNNCTCC 2 cut(s) 944, 974
Bsc4I CCNNNNNNNGG 3 cut(s) 54, 113, 133
Bse118I RCCGGY 1 cut(s) 149
Bse1I ACTGG 2 cut(s) 492, 926
Bse3DI GCAATG 2 cut(s) 176, 531
BseAI TCCGGA 1 cut(s) 164
BseBI CCWGG 4 cut(s) 55, 64, 1016, 1047
BseDI CCNNGG 5 cut(s) 711, 888, 1015, 1045, 1046
BseGI GGATG 8 cut(s) 50, 466, 496, 684, 922, 972, 1042, 1117
BseLI CCNNNNNNNGG 3 cut(s) 54, 113, 133
BseMI GCAATG 2 cut(s) 176, 531
BseMII CTCAG 1 cut(s) 621
BseNI ACTGG 2 cut(s) 492, 926
BseSI GKGCMC 3 cut(s) 1026, 1046, 1055
BseXI GCAGC 1 cut(s) 541
BshFI GGCC 1 cut(s) 420
BshNI GGYRCC 1 cut(s) 1041
BsiHKAI GWGCWC 1 cut(s) 1026
BsiSI CCGG 3 cut(s) 134, 150, 165
BslFI GGGAC 1 cut(s) 1074
BslI CCNNNNNNNGG 3 cut(s) 54, 113, 133
BsmAI GTCTC 5 cut(s) 79, 122, 378, 638, 871
BsmFI GGGAC 1 cut(s) 1074
BsnI GGCC 1 cut(s) 420
Bso31I GGTCTC 2 cut(s) 79, 122
Bsp1286I GDGCHC 3 cut(s) 1026, 1046, 1055
Bsp13I TCCGGA 1 cut(s) 164
Bsp1407I TGTACA 2 cut(s) 850, 960
Bsp143I GATC 6 cut(s) 394, 595, 707, 748, 901, 997
Bsp1720I GCTNAGC 1 cut(s) 426
BspACI CCGC 4 cut(s) 32, 214, 221, 635
BspANI GGCC 1 cut(s) 420
BspCNI CTCAG 1 cut(s) 622
BspEI TCCGGA 1 cut(s) 164
BspLI GGNNCC 6 cut(s) 163, 709, 895, 1043, 1062, 1063
BspOI GCTAGC 1 cut(s) 698
BspPI GGATC 2 cut(s) 702, 715
BspT107I GGYRCC 1 cut(s) 1041
BspTNI GGTCTC 2 cut(s) 79, 122
BsrDI GCAATG 2 cut(s) 176, 531
BsrFI RCCGGY 1 cut(s) 149
BsrGI TGTACA 2 cut(s) 850, 960
BsrI ACTGG 2 cut(s) 492, 926
BssAI RCCGGY 1 cut(s) 149
BssECI CCNNGG 5 cut(s) 711, 888, 1015, 1045, 1046
BssMI GATC 6 cut(s) 394, 595, 707, 748, 901, 997
Bst2UI CCWGG 4 cut(s) 55, 64, 1016, 1047
Bst4CI ACNGT 3 cut(s) 334, 865, 992
Bst6I CTCTTC 1 cut(s) 1067
BstAPI GCANNNNNTGC 1 cut(s) 526
BstAUI TGTACA 2 cut(s) 850, 960
BstC8I GCNNGC 3 cut(s) 518, 522, 696
BstDEI CTNAG 3 cut(s) 426, 630, 1124
BstDSI CCRYGG 1 cut(s) 711
BstEII GGTNACC 1 cut(s) 341
BstF5I GGATG 8 cut(s) 50, 466, 496, 684, 922, 972, 1042, 1117
BstHHI GCGC 1 cut(s) 685
BstKTI GATC 6 cut(s) 397, 598, 710, 751, 904, 1000
BstMAI GTCTC 5 cut(s) 79, 122, 378, 638, 871
BstMBI GATC 6 cut(s) 394, 595, 707, 748, 901, 997
BstMWI GCNNNNNNNGC 3 cut(s) 526, 691, 1050
BstNI CCWGG 4 cut(s) 55, 64, 1016, 1047
BstNSI RCATGY 2 cut(s) 482, 524
BstPI GGTNACC 1 cut(s) 341
BstSCI CCNGG 4 cut(s) 53, 62, 1014, 1045
BstSLI GKGCMC 3 cut(s) 1026, 1046, 1055
BstV1I GCAGC 1 cut(s) 541
BstV2I GAAGAC 1 cut(s) 300
BstX2I RGATCY 1 cut(s) 707
BstXI CCANNNNNNTGG 2 cut(s) 70, 815
BstYI RGATCY 1 cut(s) 707
BsuRI GGCC 1 cut(s) 420
BtgI CCRYGG 1 cut(s) 711
BtsCI GGATG 8 cut(s) 50, 466, 496, 684, 922, 972, 1042, 1117
Cac8I GCNNGC 3 cut(s) 518, 522, 696
CaiI CAGNNNCTG 1 cut(s) 535
CfoI GCGC 1 cut(s) 685
Cfr10I RCCGGY 1 cut(s) 149
Cfr13I GGNCC 1 cut(s) 1061
Csp6I GTAC 4 cut(s) 330, 792, 851, 961
CspCI CAANNNNNGTGG 2 cut(s) 540, 575
CviQI GTAC 4 cut(s) 330, 792, 851, 961
DdeI CTNAG 3 cut(s) 426, 630, 1124
DpnI GATC 6 cut(s) 396, 597, 709, 750, 903, 999
DpnII GATC 6 cut(s) 394, 595, 707, 748, 901, 997
Eam1104I CTCTTC 1 cut(s) 1067
EarI CTCTTC 1 cut(s) 1067
EciI GGCGGA 1 cut(s) 650
Eco147I AGGCCT 1 cut(s) 420
Eco31I GGTCTC 2 cut(s) 79, 122
Eco47I GGWCC 1 cut(s) 1061
Eco91I GGTNACC 1 cut(s) 341
EcoO109I RGGNCCY 1 cut(s) 1061
EcoO65I GGTNACC 1 cut(s) 341
EcoRII CCWGG 4 cut(s) 53, 62, 1014, 1045
FaqI GGGAC 1 cut(s) 1074
FauI CCCGC 1 cut(s) 39
FauNDI CATATG 1 cut(s) 589
FbaI TGATCA 2 cut(s) 394, 997
Fnu4HI GCNGC 1 cut(s) 530
FokI GGATG 8 cut(s) 37, 473, 503, 691, 929, 979, 1049, 1124
Fsp4HI GCNGC 1 cut(s) 530
FspBI CTAG 6 cut(s) 273, 347, 467, 695, 1119, 1148
GlaI GCGC 1 cut(s) 684
GluI GCNGC 1 cut(s) 530
HaeIII GGCC 1 cut(s) 420
HapII CCGG 3 cut(s) 134, 150, 165
HhaI GCGC 1 cut(s) 685
Hin6I GCGC 1 cut(s) 683
HinP1I GCGC 1 cut(s) 683
HincII GTYRAC 1 cut(s) 988
HindII GTYRAC 1 cut(s) 988
HindIII AAGCTT 1 cut(s) 1085
HinfI GANTC 3 cut(s) 103, 857, 1105
HpaI GTTAAC 1 cut(s) 988
HpaII CCGG 3 cut(s) 134, 150, 165
HphI GGTGA 7 cut(s) 139, 254, 353, 426, 574, 797, 830
Hpy166II GTNNAC 3 cut(s) 715, 988, 1024
Hpy188I TCNGA 4 cut(s) 108, 537, 997, 1139
Hpy188III TCNNGA 8 cut(s) 16, 95, 119, 165, 474, 752, 905, 1109
Hpy8I GTNNAC 3 cut(s) 715, 988, 1024
HpyAV CCTTC 2 cut(s) 964, 1095
HpyCH4III ACNGT 3 cut(s) 334, 865, 992
HpyCH4IV ACGT 3 cut(s) 449, 1003, 1031
HpyCH4V TGCA 6 cut(s) 364, 516, 524, 529, 587, 1024
HpyF10VI GCNNNNNNNGC 3 cut(s) 526, 691, 1050
HpyF3I CTNAG 3 cut(s) 426, 630, 1124
HpySE526I ACGT 3 cut(s) 449, 1003, 1031
HspAI GCGC 1 cut(s) 683
KflI GGGWCCC 1 cut(s) 1061
Kpn2I TCCGGA 1 cut(s) 164
Ksp22I TGATCA 2 cut(s) 394, 997
KspAI GTTAAC 1 cut(s) 988
Kzo9I GATC 6 cut(s) 394, 595, 707, 748, 901, 997
LmnI GCTCC 2 cut(s) 9, 66
Lsp1109I GCAGC 1 cut(s) 541
LweI GCATC 1 cut(s) 541
MaeI CTAG 6 cut(s) 273, 347, 467, 695, 1119, 1148
MaeII ACGT 3 cut(s) 449, 1003, 1031
MaeIII GTNAC 3 cut(s) 341, 499, 625
MalI GATC 6 cut(s) 396, 597, 709, 750, 903, 999
MboI GATC 6 cut(s) 394, 595, 707, 748, 901, 997
MboII GAAGA 3 cut(s) 305, 953, 1084
MfeI CAATTG 1 cut(s) 365
MflI RGATCY 1 cut(s) 707
MhlI GDGCHC 3 cut(s) 1026, 1046, 1055
MluCI AATT 4 cut(s) 21, 26, 365, 701
MmeI TCCRAC 2 cut(s) 906, 1014
MroI TCCGGA 1 cut(s) 164
MroXI GAANNNNTTC 1 cut(s) 1133
MseI TTAA 5 cut(s) 309, 372, 546, 987, 1080
MslI CAYNNNNRTG 7 cut(s) 302, 537, 672, 807, 825, 846, 852
MspI CCGG 3 cut(s) 134, 150, 165
MspR9I CCNGG 4 cut(s) 55, 64, 1016, 1047
MunI CAATTG 1 cut(s) 365
MvaI CCWGG 4 cut(s) 55, 64, 1016, 1047
MwoI GCNNNNNNNGC 3 cut(s) 526, 691, 1050
NdeI CATATG 1 cut(s) 589
NdeII GATC 6 cut(s) 394, 595, 707, 748, 901, 997
NheI GCTAGC 1 cut(s) 694
NlaIV GGNNCC 6 cut(s) 163, 709, 895, 1043, 1062, 1063
NmeAIII GCCGAG 1 cut(s) 913
NmuCI GTSAC 1 cut(s) 341
NspI RCATGY 2 cut(s) 482, 524
PaeI GCATGC 1 cut(s) 524
PasI CCCWGGG 1 cut(s) 1046
PceI AGGCCT 1 cut(s) 420
PciI ACATGT 1 cut(s) 478
PdmI GAANNNNTTC 1 cut(s) 1133
PfeI GAWTC 3 cut(s) 103, 857, 1105
PflFI GACNNNGTC 1 cut(s) 299
PfoI TCCNGGA 1 cut(s) 62
PkrI GCNGC 1 cut(s) 531
PpuMI RGGWCCY 1 cut(s) 1061
PscI ACATGT 1 cut(s) 478
Psp1406I AACGTT 2 cut(s) 449, 1003
Psp5II RGGWCCY 1 cut(s) 1061
Psp6I CCWGG 4 cut(s) 53, 62, 1014, 1045
PspEI GGTNACC 1 cut(s) 341
PspGI CCWGG 4 cut(s) 53, 62, 1014, 1045
PspN4I GGNNCC 6 cut(s) 163, 709, 895, 1043, 1062, 1063
PspPI GGNCC 1 cut(s) 1061
PspPPI RGGWCCY 1 cut(s) 1061
PstNI CAGNNNCTG 1 cut(s) 535
PsuI RGATCY 1 cut(s) 707
PsyI GACNNNGTC 1 cut(s) 299
RsaI GTAC 4 cut(s) 331, 793, 852, 962
RsaNI GTAC 4 cut(s) 330, 792, 851, 961
RseI CAYNNNNRTG 7 cut(s) 302, 537, 672, 807, 825, 846, 852
SaqAI TTAA 5 cut(s) 309, 372, 546, 987, 1080
SatI GCNGC 1 cut(s) 530
Sau3AI GATC 6 cut(s) 394, 595, 707, 748, 901, 997
Sau96I GGNCC 1 cut(s) 1061
ScrFI CCNGG 4 cut(s) 55, 64, 1016, 1047
SduI GDGCHC 3 cut(s) 1026, 1046, 1055
SfaNI GCATC 1 cut(s) 541
SinI GGWCC 1 cut(s) 1061
SmiMI CAYNNNNRTG 7 cut(s) 302, 537, 672, 807, 825, 846, 852
SmlI CTYRAG 2 cut(s) 82, 868
SmoI CTYRAG 2 cut(s) 82, 868
SpeI ACTAGT 1 cut(s) 1118
SphI GCATGC 1 cut(s) 524
Sse9I AATT 4 cut(s) 21, 26, 365, 701
SseBI AGGCCT 1 cut(s) 420
SsiI CCGC 4 cut(s) 32, 214, 221, 635
SspMI CTAG 6 cut(s) 273, 347, 467, 695, 1119, 1148
StuI AGGCCT 1 cut(s) 420
StyD4I CCNGG 4 cut(s) 53, 62, 1014, 1045
TaaI ACNGT 3 cut(s) 334, 865, 992
TaiI ACGT 3 cut(s) 452, 1006, 1034
TaqI TCGA 3 cut(s) 120, 753, 780
TasI AATT 4 cut(s) 21, 26, 365, 701
TatI WGTACW 4 cut(s) 329, 791, 850, 960
TfiI GAWTC 3 cut(s) 103, 857, 1105
Tru1I TTAA 5 cut(s) 309, 372, 546, 987, 1080
Tru9I TTAA 5 cut(s) 309, 372, 546, 987, 1080
TseFI GTSAC 1 cut(s) 341
TseI GCWGC 1 cut(s) 529
Tsp45I GTSAC 1 cut(s) 341
TspDTI ATGAA 9 cut(s) 270, 306, 555, 783, 825, 837, 983, 1084, 1128
TspGWI ACGGA 1 cut(s) 700
Tth111I GACNNNGTC 1 cut(s) 299
VneI GTGCAC 1 cut(s) 1022
VpaK11BI GGWCC 1 cut(s) 1061
XapI RAATTY 1 cut(s) 701
XceI RCATGY 2 cut(s) 482, 524
XcmI CCANNNNNNNNNTGG 1 cut(s) 1053
XmnI GAANNNNTTC 1 cut(s) 1133
XspI CTAG 6 cut(s) 273, 347, 467, 695, 1119, 1148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.