Rmu_sc0005571.1_g000036

COBW domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005571.1
OG0001734
Physical Location & Seq
Reverse (-)
108271 .. 114632
6362 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005571.1_g000036.1.cds

Sequence Viewer

Length: 1527 bp
atggcttccatatcggccgacctagccgccacccttctctccctaacttcccgtcactccatccgggtcggagccccggccctattccctctcacatggagaccccggacttccccgctgtcccataccctcagctactccacttcaacttcctcctttcgccagagactcgtctccaactctaagcggggctcgagaaggttcactgtggccgccaccactgccacgccaccgcagagtgacagctccgacgttttgacccagattccgccggataatcgtatcccggcgaccataattaccggcttcttgggctccggaaagactacattgcttaatcatatattgactgccgaccacgggaagcgaattgcagtgattgaaaatgagtatggtgaagtagacattgatggttctttggtggctgcgaaatccgccggggccgaagatattgttatgctgaataatgggtgcctttgctgcactgtgaggggtgatcttgtgcggatgatttcagacttggttaataagaagaaagggaggtttgatcatattgtgatagagactacaggattggcaaatccagcaccgataattcaaacattttatgcggaagatcagattttcaatgatgtcaagttggacggtgttgtcacgttagttgatgctaaacatgctcgacttcatctggatgaagtcaaggcagaaggagtggttaatgaggcagtagagcagattgcttatgctgatcgtatcattgtgaataagactgatcttgttggcgagtcagatattgcttctttggtgcagagaataaggaacattaatggcatggctcaactgaagcgtacagagtttggaaaagttaacctggattatgtgctcggtatcggaggatttgatttggagaggatagagagtgctattgacgctgaagctgagaaggaagatcatgaccatcatcatgaccaccatgaccatcatcatcatcatcaccatcatgatgaagaacatgatcacaaacatgaacatcatgatggtcaccactcccatgatcatgttcatgatcctggtgtttcttctgtcagtattgtttgcgaagggaacctcgatctggagaaggccaacatgtggcttggcacactgttgttggaccggagtgaggacatatatcgtatgaaaggtcttctatctgttcagggtatggatgagagatttgtcttccagggagttcatgatatcttccaaggttctcccgatcggttgtggggccccaatgagccaagaacaaataagattgttttcatagggaagaacttggatgcacaagagttggagaaggcaagggaattgcacgctgccagccaagcctggcccaatccattgtcggccgctgccgacagatcagatcaccacctcgccagaaatgccggaccgccaccatcatggcggcatcgccctcacgcagtggatacgccctgcccttatcaaaaagtcccgtccccagatcagatccaagagttctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CobW_C cobW

Protein Analysis

508

Amino Acids

56.32

Weight (kDa)

5.97

Isoelectric Point (pI)

34.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 650
AccB1I GGYRCC 1 cut(s) 471
AccB7I CCANNNNNTGG 1 cut(s) 1131
AccI GTMKAC 1 cut(s) 402
AccIII TCCGGA 1 cut(s) 317
AclWI GGATC 2 cut(s) 1061, 1507
AcoI YGGCCR 3 cut(s) 15, 210, 1389
AcuI CTGAAG 2 cut(s) 863, 954
AdeI CACNNNGTG 1 cut(s) 1468
AfaI GTAC 1 cut(s) 850
AfiI CCNNNNNNNGG 4 cut(s) 360, 1114, 1131, 1162
AflIII ACRYGT 1 cut(s) 1128
AgsI TTSAA 4 cut(s) 147, 383, 599, 628
AjnI CCWGG 4 cut(s) 870, 1069, 1224, 1370
AluBI AGCT 3 cut(s) 135, 246, 938
AluI AGCT 3 cut(s) 135, 246, 938
Alw21I GWGCWC 1 cut(s) 885
Alw26I GTCTC 4 cut(s) 94, 160, 178, 557
AlwI GGATC 2 cut(s) 1061, 1507
Ama87I CYCGRG 1 cut(s) 193
Aor13HI TCCGGA 1 cut(s) 317
AoxI GGCC 8 cut(s) 15, 78, 210, 441, 1122, 1270, 1373, 1389
ApaI GGGCCC 1 cut(s) 1274
ApeKI GCWGC 4 cut(s) 425, 480, 1358, 1394
AseI ATTAAT 1 cut(s) 825
AspS9I GGNCC 7 cut(s) 79, 441, 1153, 1270, 1271, 1374, 1433
AsuC2I CCSGG 5 cut(s) 65, 77, 106, 287, 439
AsuHPI GGTGA 5 cut(s) 407, 506, 986, 1034, 1403
AvaI CYCGRG 1 cut(s) 193
AvaII GGWCC 2 cut(s) 1153, 1433
BaeGI GKGCMC 1 cut(s) 1274
BanI GGYRCC 1 cut(s) 471
BanII GRGCYC 4 cut(s) 76, 194, 317, 1274
BbsI GAAGAC 2 cut(s) 1178, 1213
Bbv12I GWGCWC 1 cut(s) 885
BbvCI CCTCAGC 1 cut(s) 131
BbvI GCAGC 4 cut(s) 412, 467, 1345, 1381
BccI CCATC 7 cut(s) 68, 404, 966, 987, 1005, 1031, 1450
BciT130I CCWGG 4 cut(s) 872, 1071, 1226, 1372
BciVI GTATCC 2 cut(s) 293, 1465
BclI TGATCA 3 cut(s) 547, 1015, 1054
BcnI CCSGG 5 cut(s) 65, 77, 106, 287, 439
BcoDI GTCTC 4 cut(s) 94, 160, 178, 557
BfaI CTAG 1 cut(s) 23
BfmI CTRYAG 1 cut(s) 567
BfuI GTATCC 2 cut(s) 293, 1465
BisI GCNGC 8 cut(s) 27, 213, 426, 481, 1359, 1392, 1395, 1451
BlsI GCNGC 8 cut(s) 28, 214, 427, 482, 1360, 1393, 1396, 1452
Bme1390I CCNGG 9 cut(s) 65, 77, 106, 287, 439, 872, 1071, 1226, 1372
Bme18I GGWCC 2 cut(s) 1153, 1433
BmeT110I CYCGRG 1 cut(s) 193
BmgT120I GGNCC 7 cut(s) 79, 441, 1153, 1270, 1271, 1374, 1433
BmiI GGNNCC 8 cut(s) 73, 316, 442, 473, 1106, 1271, 1272, 1273
BmrFI CCNGG 9 cut(s) 65, 77, 106, 287, 439, 872, 1071, 1226, 1372
BmsI GCATC 3 cut(s) 655, 1312, 1462
BpiI GAAGAC 2 cut(s) 1178, 1213
BpmI CTGGAG 1 cut(s) 1136
Bpu10I CCTNAGC 1 cut(s) 131
BpuMI CCSGG 5 cut(s) 65, 77, 106, 287, 439
BsaI GGTCTC 1 cut(s) 94
BsaJI CCNNGG 6 cut(s) 75, 104, 358, 438, 1225, 1246
BsaWI WCCGGW 2 cut(s) 317, 1155
Bsc4I CCNNNNNNNGG 4 cut(s) 360, 1114, 1131, 1162
Bse118I RCCGGY 1 cut(s) 302
Bse3DI GCAATG 1 cut(s) 329
BseAI TCCGGA 1 cut(s) 317
BseBI CCWGG 4 cut(s) 872, 1071, 1226, 1372
BseDI CCNNGG 6 cut(s) 75, 104, 358, 438, 1225, 1246
BseGI GGATG 5 cut(s) 60, 513, 697, 1213, 1327
BseLI CCNNNNNNNGG 4 cut(s) 360, 1114, 1131, 1162
BseMI GCAATG 1 cut(s) 329
BseMII CTCAG 2 cut(s) 145, 930
BseSI GKGCMC 1 cut(s) 1274
BseX3I CGGCCG 2 cut(s) 15, 1389
BseXI GCAGC 4 cut(s) 412, 467, 1345, 1381
BsgI GTGCAG 2 cut(s) 466, 827
Bsh1285I CGRYCG 3 cut(s) 18, 1261, 1392
BshFI GGCC 8 cut(s) 17, 80, 212, 443, 1124, 1272, 1375, 1391
BshNI GGYRCC 1 cut(s) 471
BsiEI CGRYCG 3 cut(s) 18, 1261, 1392
BsiHKAI GWGCWC 1 cut(s) 885
BsiHKCI CYCGRG 1 cut(s) 193
BslFI GGGAC 3 cut(s) 106, 1481, 1486
BslI CCNNNNNNNGG 4 cut(s) 360, 1114, 1131, 1162
BsmAI GTCTC 4 cut(s) 94, 160, 178, 557
BsmBI CGTCTC 1 cut(s) 178
BsmFI GGGAC 3 cut(s) 106, 1481, 1486
BsnI GGCC 8 cut(s) 17, 80, 212, 443, 1124, 1272, 1375, 1391
Bso31I GGTCTC 1 cut(s) 94
BsoBI CYCGRG 1 cut(s) 193
Bsp120I GGGCCC 1 cut(s) 1270
Bsp1286I GDGCHC 5 cut(s) 76, 194, 317, 885, 1274
Bsp13I TCCGGA 1 cut(s) 317
BspANI GGCC 8 cut(s) 17, 80, 212, 443, 1124, 1272, 1375, 1391
BspCNI CTCAG 2 cut(s) 144, 931
BspEI TCCGGA 1 cut(s) 317
BspHI TCATGA 6 cut(s) 952, 964, 1000, 1033, 1063, 1234
BspLI GGNNCC 8 cut(s) 73, 316, 442, 473, 1106, 1271, 1272, 1273
BspPI GGATC 2 cut(s) 1061, 1507
BspT107I GGYRCC 1 cut(s) 471
BspTNI GGTCTC 1 cut(s) 94
BsrDI GCAATG 1 cut(s) 329
BsrFI RCCGGY 1 cut(s) 302
BssAI RCCGGY 1 cut(s) 302
BssECI CCNNGG 6 cut(s) 75, 104, 358, 438, 1225, 1246
BssT1I CCWWGG 1 cut(s) 1246
Bst2UI CCWGG 4 cut(s) 872, 1071, 1226, 1372
Bst4CI ACNGT 4 cut(s) 208, 487, 647, 1146
BstC8I GCNNGC 2 cut(s) 1356, 1363
BstDEI CTNAG 3 cut(s) 131, 183, 939
BstDSI CCRYGG 1 cut(s) 358
BstEII GGTNACC 1 cut(s) 1040
BstF5I GGATG 5 cut(s) 60, 513, 697, 1213, 1327
BstMAI GTCTC 4 cut(s) 94, 160, 178, 557
BstMCI CGRYCG 3 cut(s) 18, 1261, 1392
BstNI CCWGG 4 cut(s) 872, 1071, 1226, 1372
BstNSI RCATGY 2 cut(s) 677, 1132
BstPI GGTNACC 1 cut(s) 1040
BstSCI CCNGG 9 cut(s) 63, 75, 104, 285, 437, 870, 1069, 1224, 1370
BstSFI CTRYAG 1 cut(s) 567
BstSLI GKGCMC 1 cut(s) 1274
BstV1I GCAGC 4 cut(s) 412, 467, 1345, 1381
BstV2I GAAGAC 2 cut(s) 1178, 1213
BstX2I RGATCY 1 cut(s) 1512
BstXI CCANNNNNNTGG 1 cut(s) 1446
BstYI RGATCY 1 cut(s) 1512
BstZI CGGCCG 2 cut(s) 15, 1389
BsuI GTATCC 2 cut(s) 293, 1465
BsuRI GGCC 8 cut(s) 17, 80, 212, 443, 1124, 1272, 1375, 1391
BtgI CCRYGG 1 cut(s) 358
BtgZI GCGATG 1 cut(s) 1439
BtsCI GGATG 5 cut(s) 60, 513, 697, 1213, 1327
BtsI GCAGTG 3 cut(s) 219, 381, 1473
BtsIMutI CAGTG 6 cut(s) 204, 219, 381, 483, 1142, 1473
Cac8I GCNNGC 2 cut(s) 1356, 1363
CciI TCATGA 6 cut(s) 952, 964, 1000, 1033, 1063, 1234
Cfr10I RCCGGY 1 cut(s) 302
Cfr13I GGNCC 7 cut(s) 79, 441, 1153, 1270, 1271, 1374, 1433
CpoI CGGWCCG 1 cut(s) 1433
CseI GACGC 1 cut(s) 938
Csp6I GTAC 1 cut(s) 849
CspI CGGWCCG 1 cut(s) 1433
CviQI GTAC 1 cut(s) 849
DdeI CTNAG 3 cut(s) 131, 183, 939
DraIII CACNNNGTG 1 cut(s) 1468
DrdI GACNNNNNNGTC 1 cut(s) 650
DseDI GACNNNNNNGTC 1 cut(s) 650
EaeI YGGCCR 3 cut(s) 15, 210, 1389
EagI CGGCCG 2 cut(s) 15, 1389
EciI GGCGGA 2 cut(s) 258, 424
EclXI CGGCCG 2 cut(s) 15, 1389
Eco130I CCWWGG 1 cut(s) 1246
Eco24I GRGCYC 4 cut(s) 76, 194, 317, 1274
Eco31I GGTCTC 1 cut(s) 94
Eco32I GATATC 1 cut(s) 1240
Eco47I GGWCC 2 cut(s) 1153, 1433
Eco52I CGGCCG 2 cut(s) 15, 1389
Eco57I CTGAAG 2 cut(s) 863, 954
Eco88I CYCGRG 1 cut(s) 193
Eco91I GGTNACC 1 cut(s) 1040
EcoO109I RGGNCCY 2 cut(s) 1270, 1271
EcoO65I GGTNACC 1 cut(s) 1040
EcoRII CCWGG 4 cut(s) 870, 1069, 1224, 1370
EcoRV GATATC 1 cut(s) 1240
EcoT14I CCWWGG 1 cut(s) 1246
EcoT38I GRGCYC 4 cut(s) 76, 194, 317, 1274
ErhI CCWWGG 1 cut(s) 1246
Esp3I CGTCTC 1 cut(s) 178
FaqI GGGAC 3 cut(s) 106, 1481, 1486
FauI CCCGC 2 cut(s) 123, 180
FbaI TGATCA 3 cut(s) 547, 1015, 1054
FblI GTMKAC 1 cut(s) 402
Fnu4HI GCNGC 8 cut(s) 27, 213, 426, 481, 1359, 1392, 1395, 1451
FokI GGATG 5 cut(s) 47, 520, 704, 1220, 1334
FriOI GRGCYC 4 cut(s) 76, 194, 317, 1274
Fsp4HI GCNGC 8 cut(s) 27, 213, 426, 481, 1359, 1392, 1395, 1451
FspBI CTAG 1 cut(s) 23
GluI GCNGC 8 cut(s) 27, 213, 426, 481, 1359, 1392, 1395, 1451
GsuI CTGGAG 1 cut(s) 1136
HaeIII GGCC 8 cut(s) 17, 80, 212, 443, 1124, 1272, 1375, 1391
HgaI GACGC 1 cut(s) 938
HincII GTYRAC 1 cut(s) 868
HindII GTYRAC 1 cut(s) 868
HinfI GANTC 3 cut(s) 168, 265, 785
HpaI GTTAAC 1 cut(s) 868
HphI GGTGA 5 cut(s) 407, 506, 986, 1034, 1403
Hpy166II GTNNAC 3 cut(s) 204, 403, 868
Hpy188I TCNGA 9 cut(s) 71, 250, 517, 621, 790, 893, 1408, 1512, 1526
Hpy8I GTNNAC 3 cut(s) 204, 403, 868
Hpy99I CGWCG 1 cut(s) 254
HpyAV CCTTC 7 cut(s) 44, 192, 701, 937, 1094, 1114, 1333
HpyCH4III ACNGT 4 cut(s) 208, 487, 647, 1146
HpyCH4IV ACGT 2 cut(s) 252, 656
HpyCH4V TGCA 5 cut(s) 374, 483, 808, 1325, 1354
HpyF3I CTNAG 3 cut(s) 131, 183, 939
HpySE526I ACGT 2 cut(s) 252, 656
Kpn2I TCCGGA 1 cut(s) 317
Ksp22I TGATCA 3 cut(s) 547, 1015, 1054
KspAI GTTAAC 1 cut(s) 868
LmnI GCTCC 3 cut(s) 71, 251, 320
Lsp1109I GCAGC 4 cut(s) 412, 467, 1345, 1381
LweI GCATC 3 cut(s) 655, 1312, 1462
MaeI CTAG 1 cut(s) 23
MaeII ACGT 2 cut(s) 252, 656
MaeIII GTNAC 4 cut(s) 53, 239, 652, 1040
MflI RGATCY 1 cut(s) 1512
MhlI GDGCHC 5 cut(s) 76, 194, 317, 885, 1274
MluCI AATT 4 cut(s) 297, 369, 594, 1349
MlyI GAGTC 2 cut(s) 162, 794
MmeI TCCRAC 6 cut(s) 49, 201, 273, 621, 1131, 1314
MroI TCCGGA 1 cut(s) 317
MseI TTAA 5 cut(s) 336, 525, 717, 825, 867
MspA1I CMGCKG 2 cut(s) 118, 1394
MspR9I CCNGG 9 cut(s) 65, 77, 106, 287, 439, 872, 1071, 1226, 1372
MvaI CCWGG 4 cut(s) 872, 1071, 1226, 1372
NciI CCSGG 5 cut(s) 65, 77, 106, 287, 439
NlaIV GGNNCC 8 cut(s) 73, 316, 442, 473, 1106, 1271, 1272, 1273
NmuCI GTSAC 4 cut(s) 53, 239, 652, 1040
NspI RCATGY 2 cut(s) 677, 1132
PaeR7I CTCGAG 1 cut(s) 193
PagI TCATGA 6 cut(s) 952, 964, 1000, 1033, 1063, 1234
PciI ACATGT 1 cut(s) 1128
PfeI GAWTC 1 cut(s) 265
PflMI CCANNNNNTGG 1 cut(s) 1131
PkrI GCNGC 8 cut(s) 28, 214, 427, 482, 1360, 1393, 1396, 1452
Ple19I CGATCG 1 cut(s) 1261
PleI GAGTC 2 cut(s) 162, 793
PpsI GAGTC 2 cut(s) 162, 793
PscI ACATGT 1 cut(s) 1128
PshBI ATTAAT 1 cut(s) 825
Psp6I CCWGG 4 cut(s) 870, 1069, 1224, 1370
PspEI GGTNACC 1 cut(s) 1040
PspGI CCWGG 4 cut(s) 870, 1069, 1224, 1370
PspN4I GGNNCC 8 cut(s) 73, 316, 442, 473, 1106, 1271, 1272, 1273
PspOMI GGGCCC 1 cut(s) 1270
PspPI GGNCC 7 cut(s) 79, 441, 1153, 1270, 1271, 1374, 1433
PsuI RGATCY 1 cut(s) 1512
PvuI CGATCG 1 cut(s) 1261
RsaI GTAC 1 cut(s) 850
RsaNI GTAC 1 cut(s) 849
Rsr2I CGGWCCG 1 cut(s) 1433
RsrII CGGWCCG 1 cut(s) 1433
SaqAI TTAA 5 cut(s) 336, 525, 717, 825, 867
SatI GCNGC 8 cut(s) 27, 213, 426, 481, 1359, 1392, 1395, 1451
Sau96I GGNCC 7 cut(s) 79, 441, 1153, 1270, 1271, 1374, 1433
SchI GAGTC 2 cut(s) 162, 794
ScrFI CCNGG 9 cut(s) 65, 77, 106, 287, 439, 872, 1071, 1226, 1372
SduI GDGCHC 5 cut(s) 76, 194, 317, 885, 1274
SfaNI GCATC 3 cut(s) 655, 1312, 1462
SfcI CTRYAG 1 cut(s) 567
Sfr274I CTCGAG 1 cut(s) 193
SinI GGWCC 2 cut(s) 1153, 1433
SlaI CTCGAG 1 cut(s) 193
SmlI CTYRAG 1 cut(s) 193
SmoI CTYRAG 1 cut(s) 193
Sse9I AATT 4 cut(s) 297, 369, 594, 1349
SspMI CTAG 1 cut(s) 23
StyD4I CCNGG 9 cut(s) 63, 75, 104, 285, 437, 870, 1069, 1224, 1370
StyI CCWWGG 1 cut(s) 1246
TaaI ACNGT 4 cut(s) 208, 487, 647, 1146
TaiI ACGT 2 cut(s) 255, 659
TaqI TCGA 3 cut(s) 194, 679, 1110
TasI AATT 4 cut(s) 297, 369, 594, 1349
TauI GCSGC 4 cut(s) 29, 215, 1394, 1453
TfiI GAWTC 1 cut(s) 265
Tru1I TTAA 5 cut(s) 336, 525, 717, 825, 867
Tru9I TTAA 5 cut(s) 336, 525, 717, 825, 867
TscAI CASTG 6 cut(s) 211, 226, 381, 490, 1149, 1473
TseFI GTSAC 4 cut(s) 53, 239, 652, 1040
TseI GCWGC 4 cut(s) 425, 480, 1358, 1394
Tsp45I GTSAC 4 cut(s) 53, 239, 652, 1040
TspDTI ATGAA 8 cut(s) 674, 708, 1020, 1041, 1052, 1193, 1223, 1294
TspRI CASTG 6 cut(s) 211, 226, 381, 490, 1149, 1473
Van91I CCANNNNNTGG 1 cut(s) 1131
VpaK11BI GGWCC 2 cut(s) 1153, 1433
VspI ATTAAT 1 cut(s) 825
XceI RCATGY 2 cut(s) 677, 1132
XhoI CTCGAG 1 cut(s) 193
XmiI GTMKAC 1 cut(s) 402
XspI CTAG 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.