FvH4_6g29200

COBW domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001734
Physical Location & Seq
Forward (+)
22535022 .. 22542781
7760 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g29200.t2

Sequence Viewer

Length: 1374 bp
ATGGCTTCAATATCGGCCGACCTAGCCGCCACCCTTCTCGGGCTCACTTCCCGCCACTCCGCCTGGGTCGGAGCCCCGGCCCTATTTTCATGGAGACCCAGGACTTCCGCGCTACGCTACTCAACTTCAACTTCCTCGTTTCGCCACAGACTCGTCTCCAAACCTAAGCGAGTCTCAAGAAGGTTCTCTGTGGCCGCCACCACTGCCTCACCGCCGCAGAGCGACGGCTCCGACGTCTTGACGCAGATTCCTCCGGATAATCGTATCCCGGCGACGATAATTACCGGCTTCTTGGGCTCCGGAAAGACTACGTTGCTTAACCATATATTGACTGCGGACCACGGGAAGCGAATTGCTGTGATTGAAAATGAGTATGGTGAAGTAGACATTGATGGTTCTTTGGTGGCTGCGAAATCGGCTGGGGCGGAAGATATTGTAATGTTGAATAATGGGTGCCTGTGCTGCACTGTGAGGGGTGATCTTGTGCGGATGATTTCGGAGTTGGTTAATAAGAAGAAAGGGAGGTTCGATCATATTGTGATAGAGACTACGGGATTGGCAAATCCAGCACCGATAATTCAAACATTTTATGCTGAAGATCAGGTTTTTAATGATGTCAAGTTGGACGGTGTTGTCACGTTAGTTGATGCTAAACATGCTCGACTTCATCTGGATGAAGTCAAGCCAGAAGGAGTTGTTAATGAGGCAGTAGAGCAGATTGCTTATGCTGATCGTATCATTGTGAATAAGACTGATCTTGTTGGCGAGCCAGATATTGCTTCTTTGGTGCAGCGAATAAGGAACATCAATGGCATGGCTCAACTGAAGCGTACAGAGTTTGGAAAAGTTAACCTGGATTATGTACTTGGCATCGGAGGATTTGATTTGGAGAGGATAGAGAGCGCTATTGATGCTGAAGGTGAGAAGGAGGATCACGATCACCATAATCATGATGACCATCACCACGATCATCATCACCATCATGATGAAGAACATGATCACAAACATGAACATCACGATGGCCACCACTCCCATGATCATGTCCATGATCCTGGTGTTTCTTCTGTCAGTATTGTATGTGAAGGGAACTTGGATCTTGAGAAGGCCAACATGTGGCTCGGCACACTGTTGTTGGAGCGGAGTGAGGATATATATCGGATGAAAGGTCTTCTATCTGTTCAGGGTATGGATGAGCGATTTGTCTTCCAGGGAGTTCATGATATATTCCAAGGTTCACCTGATCGATTGTGGGGCCCTGACGAGCCAAGAACAAATAAGATTGTTTTCATAGGGAAAAACTTAGATGCACAGGAGCTGGAAAAGGGTTTTAAAGGCTGTTTACTGAGAAATAAGTATTCAAATTACCAGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CobW_C cobW

Protein Analysis

458

Amino Acids

50.79

Weight (kDa)

5.89

Isoelectric Point (pI)

33.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cobW PF02492 90 - 274 3.6e-59 CobW/HypB/UreG, nucleotide-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 632
AatII GACGTC 1 cut(s) 237
AccB1I GGYRCC 1 cut(s) 453
AccB7I CCANNNNNTGG 1 cut(s) 1113
AccBSI CCGCTC 1 cut(s) 1138
AccI GTMKAC 1 cut(s) 384
AccII CGCG 1 cut(s) 110
AccIII TCCGGA 2 cut(s) 253, 299
AclWI GGATC 3 cut(s) 939, 1043, 1101
AcoI YGGCCR 3 cut(s) 15, 192, 1021
AcuI CTGAAG 3 cut(s) 615, 845, 936
AcyI GRCGYC 1 cut(s) 234
AfaI GTAC 2 cut(s) 832, 864
AfeI AGCGCT 1 cut(s) 904
AfiI CCNNNNNNNGG 2 cut(s) 39, 1113
AflIII ACRYGT 1 cut(s) 1110
AgsI TTSAA 6 cut(s) 9, 129, 365, 445, 581, 1359
AjnI CCWGG 5 cut(s) 62, 98, 852, 1051, 1206
AluBI AGCT 1 cut(s) 1315
AluI AGCT 1 cut(s) 1315
Alw26I GTCTC 4 cut(s) 88, 160, 178, 539
AlwI GGATC 3 cut(s) 939, 1043, 1101
AlwNI CAGNNNCTG 1 cut(s) 1315
Ama87I CYCGRG 1 cut(s) 38
Aor13HI TCCGGA 2 cut(s) 253, 299
Aor51HI AGCGCT 1 cut(s) 904
AoxI GGCC 6 cut(s) 15, 78, 192, 1021, 1104, 1252
ApaI GGGCCC 1 cut(s) 1256
ApeKI GCWGC 3 cut(s) 407, 462, 790
AspLEI GCGC 2 cut(s) 112, 905
AspS9I GGNCC 4 cut(s) 79, 337, 1252, 1253
AsuC2I CCSGG 2 cut(s) 77, 269
AsuHPI GGTGA 8 cut(s) 201, 389, 488, 932, 932, 953, 968, 1227
AvaI CYCGRG 1 cut(s) 38
AvaII GGWCC 1 cut(s) 337
BaeGI GKGCMC 1 cut(s) 1256
BalI TGGCCA 1 cut(s) 1023
BanI GGYRCC 1 cut(s) 453
BanII GRGCYC 4 cut(s) 45, 76, 299, 1256
BbsI GAAGAC 2 cut(s) 1160, 1195
BbvI GCAGC 3 cut(s) 394, 449, 802
BccI CCATC 4 cut(s) 386, 966, 987, 1013
BceAI ACGGC 1 cut(s) 241
BciT130I CCWGG 5 cut(s) 64, 100, 854, 1053, 1208
BciVI GTATCC 1 cut(s) 275
BclI TGATCA 2 cut(s) 997, 1036
BcnI CCSGG 2 cut(s) 77, 269
BcoDI GTCTC 4 cut(s) 88, 160, 178, 539
BfaI CTAG 1 cut(s) 23
BfoI RGCGCY 1 cut(s) 906
BfuI GTATCC 1 cut(s) 275
BisI GCNGC 6 cut(s) 27, 195, 215, 408, 463, 791
BlsI GCNGC 6 cut(s) 28, 196, 216, 409, 464, 792
Bme1390I CCNGG 7 cut(s) 64, 77, 100, 269, 854, 1053, 1208
Bme18I GGWCC 1 cut(s) 337
BmeT110I CYCGRG 1 cut(s) 38
BmgT120I GGNCC 4 cut(s) 79, 337, 1252, 1253
BmiI GGNNCC 6 cut(s) 73, 229, 298, 455, 1253, 1254
BmrFI CCNGG 7 cut(s) 64, 77, 100, 269, 854, 1053, 1208
BmsI GCATC 4 cut(s) 637, 879, 901, 1294
BpiI GAAGAC 2 cut(s) 1160, 1195
Bpu10I CCTNAGC 1 cut(s) 165
BpuEI CTTGAG 2 cut(s) 160, 1118
BpuMI CCSGG 2 cut(s) 77, 269
Bsa29I ATCGAT 1 cut(s) 1243
BsaBI GATNNNNATC 4 cut(s) 936, 957, 972, 1152
BsaHI GRCGYC 1 cut(s) 234
BsaI GGTCTC 1 cut(s) 88
BsaJI CCNNGG 6 cut(s) 63, 75, 98, 340, 1207, 1228
BsaWI WCCGGW 2 cut(s) 253, 299
Bsc4I CCNNNNNNNGG 2 cut(s) 39, 1113
Bse118I RCCGGY 1 cut(s) 284
Bse8I GATNNNNATC 4 cut(s) 936, 957, 972, 1152
BseAI TCCGGA 2 cut(s) 253, 299
BseBI CCWGG 5 cut(s) 64, 100, 854, 1053, 1208
BseCI ATCGAT 1 cut(s) 1243
BseDI CCNNGG 6 cut(s) 63, 75, 98, 340, 1207, 1228
BseGI GGATG 4 cut(s) 495, 679, 1164, 1195
BseJI GATNNNNATC 4 cut(s) 936, 957, 972, 1152
BseLI CCNNNNNNNGG 2 cut(s) 39, 1113
BseMII CTCAG 1 cut(s) 1334
BseSI GKGCMC 1 cut(s) 1256
BseX3I CGGCCG 1 cut(s) 15
BseXI GCAGC 3 cut(s) 394, 449, 802
BseYI CCCAGC 1 cut(s) 419
BsgI GTGCAG 2 cut(s) 448, 809
Bsh1236I CGCG 1 cut(s) 110
Bsh1285I CGRYCG 1 cut(s) 18
BshFI GGCC 6 cut(s) 17, 80, 194, 1023, 1106, 1254
BshNI GGYRCC 1 cut(s) 453
BshVI ATCGAT 1 cut(s) 1243
BsiEI CGRYCG 1 cut(s) 18
BsiHKCI CYCGRG 1 cut(s) 38
BsiSI CCGG 5 cut(s) 77, 254, 269, 285, 300
BslI CCNNNNNNNGG 2 cut(s) 39, 1113
BsmAI GTCTC 4 cut(s) 88, 160, 178, 539
BsmBI CGTCTC 1 cut(s) 160
BsnI GGCC 6 cut(s) 17, 80, 194, 1023, 1106, 1254
Bso31I GGTCTC 1 cut(s) 88
BsoBI CYCGRG 1 cut(s) 38
Bsp120I GGGCCC 1 cut(s) 1252
Bsp1286I GDGCHC 4 cut(s) 45, 76, 299, 1256
Bsp13I TCCGGA 2 cut(s) 253, 299
BspANI GGCC 6 cut(s) 17, 80, 194, 1023, 1106, 1254
BspCNI CTCAG 1 cut(s) 1335
BspDI ATCGAT 1 cut(s) 1243
BspEI TCCGGA 2 cut(s) 253, 299
BspFNI CGCG 1 cut(s) 110
BspHI TCATGA 3 cut(s) 949, 982, 1216
BspLI GGNNCC 6 cut(s) 73, 229, 298, 455, 1253, 1254
BspPI GGATC 3 cut(s) 939, 1043, 1101
BspT107I GGYRCC 1 cut(s) 453
BspTNI GGTCTC 1 cut(s) 88
BsrBI CCGCTC 1 cut(s) 1138
BsrFI RCCGGY 1 cut(s) 284
BssAI RCCGGY 1 cut(s) 284
BssECI CCNNGG 6 cut(s) 63, 75, 98, 340, 1207, 1228
BssNI GRCGYC 1 cut(s) 234
BssT1I CCWWGG 1 cut(s) 1228
Bst2UI CCWGG 5 cut(s) 64, 100, 854, 1053, 1208
Bst4CI ACNGT 3 cut(s) 469, 629, 1128
BstACI GRCGYC 1 cut(s) 234
BstC8I GCNNGC 1 cut(s) 767
BstDEI CTNAG 3 cut(s) 165, 1300, 1343
BstDSI CCRYGG 1 cut(s) 340
BstF5I GGATG 4 cut(s) 495, 679, 1164, 1195
BstFNI CGCG 1 cut(s) 110
BstH2I RGCGCY 1 cut(s) 906
BstHHI GCGC 2 cut(s) 112, 905
BstMAI GTCTC 4 cut(s) 88, 160, 178, 539
BstMCI CGRYCG 1 cut(s) 18
BstMWI GCNNNNNNNGC 8 cut(s) 23, 203, 294, 416, 462, 566, 656, 911
BstNI CCWGG 5 cut(s) 64, 100, 854, 1053, 1208
BstNSI RCATGY 2 cut(s) 659, 1114
BstSCI CCNGG 7 cut(s) 62, 75, 98, 267, 852, 1051, 1206
BstSLI GKGCMC 1 cut(s) 1256
BstUI CGCG 1 cut(s) 110
BstV1I GCAGC 3 cut(s) 394, 449, 802
BstV2I GAAGAC 2 cut(s) 1160, 1195
BstX2I RGATCY 1 cut(s) 1093
BstXI CCANNNNNNTGG 1 cut(s) 1052
BstYI RGATCY 1 cut(s) 1093
BstZI CGGCCG 1 cut(s) 15
Bsu15I ATCGAT 1 cut(s) 1243
BsuI GTATCC 1 cut(s) 275
BsuRI GGCC 6 cut(s) 17, 80, 194, 1023, 1106, 1254
BsuTUI ATCGAT 1 cut(s) 1243
BtgI CCRYGG 1 cut(s) 340
BtsCI GGATG 4 cut(s) 495, 679, 1164, 1195
BtsI GCAGTG 1 cut(s) 201
BtsIMutI CAGTG 3 cut(s) 201, 465, 1124
Cac8I GCNNGC 1 cut(s) 767
CaiI CAGNNNCTG 1 cut(s) 1315
CciI TCATGA 3 cut(s) 949, 982, 1216
CfoI GCGC 2 cut(s) 112, 905
Cfr10I RCCGGY 1 cut(s) 284
Cfr13I GGNCC 4 cut(s) 79, 337, 1252, 1253
ClaI ATCGAT 1 cut(s) 1243
CseI GACGC 1 cut(s) 250
Csp6I GTAC 2 cut(s) 831, 863
CviQI GTAC 2 cut(s) 831, 863
DdeI CTNAG 3 cut(s) 165, 1300, 1343
DraI TTTAAA 1 cut(s) 1330
DrdI GACNNNNNNGTC 1 cut(s) 632
DseDI GACNNNNNNGTC 1 cut(s) 632
EaeI YGGCCR 3 cut(s) 15, 192, 1021
EagI CGGCCG 1 cut(s) 15
EciI GGCGGA 2 cut(s) 49, 440
EclXI CGGCCG 1 cut(s) 15
Eco130I CCWWGG 1 cut(s) 1228
Eco24I GRGCYC 4 cut(s) 45, 76, 299, 1256
Eco31I GGTCTC 1 cut(s) 88
Eco47I GGWCC 1 cut(s) 337
Eco47III AGCGCT 1 cut(s) 904
Eco52I CGGCCG 1 cut(s) 15
Eco57I CTGAAG 3 cut(s) 615, 845, 936
Eco88I CYCGRG 1 cut(s) 38
EcoO109I RGGNCCY 2 cut(s) 1252, 1253
EcoRII CCWGG 5 cut(s) 62, 98, 852, 1051, 1206
EcoT14I CCWWGG 1 cut(s) 1228
EcoT38I GRGCYC 4 cut(s) 45, 76, 299, 1256
ErhI CCWWGG 1 cut(s) 1228
Esp3I CGTCTC 1 cut(s) 160
FauI CCCGC 1 cut(s) 59
FbaI TGATCA 2 cut(s) 997, 1036
FblI GTMKAC 1 cut(s) 384
Fnu4HI GCNGC 6 cut(s) 27, 195, 215, 408, 463, 791
FokI GGATG 4 cut(s) 502, 686, 1171, 1202
FriOI GRGCYC 4 cut(s) 45, 76, 299, 1256
Fsp4HI GCNGC 6 cut(s) 27, 195, 215, 408, 463, 791
FspBI CTAG 1 cut(s) 23
GlaI GCGC 2 cut(s) 111, 904
GluI GCNGC 6 cut(s) 27, 195, 215, 408, 463, 791
GsaI CCCAGC 1 cut(s) 423
HaeII RGCGCY 1 cut(s) 906
HaeIII GGCC 6 cut(s) 17, 80, 194, 1023, 1106, 1254
HapII CCGG 5 cut(s) 77, 254, 269, 285, 300
HgaI GACGC 1 cut(s) 250
HhaI GCGC 2 cut(s) 112, 905
Hin1I GRCGYC 1 cut(s) 234
Hin6I GCGC 2 cut(s) 110, 903
HinP1I GCGC 2 cut(s) 110, 903
HincII GTYRAC 1 cut(s) 850
HindII GTYRAC 1 cut(s) 850
HinfI GANTC 3 cut(s) 150, 171, 247
HpaI GTTAAC 1 cut(s) 850
HpaII CCGG 5 cut(s) 77, 254, 269, 285, 300
HphI GGTGA 8 cut(s) 201, 389, 488, 932, 932, 953, 968, 1227
Hpy166II GTNNAC 4 cut(s) 385, 850, 1235, 1340
Hpy188I TCNGA 5 cut(s) 71, 232, 499, 875, 1158
Hpy8I GTNNAC 4 cut(s) 385, 850, 1235, 1340
Hpy99I CGWCG 3 cut(s) 227, 236, 277
HpyAV CCTTC 7 cut(s) 44, 174, 683, 911, 919, 1076, 1096
HpyCH4III ACNGT 3 cut(s) 469, 629, 1128
HpyCH4IV ACGT 3 cut(s) 234, 311, 638
HpyCH4V TGCA 3 cut(s) 465, 790, 1307
HpyF10VI GCNNNNNNNGC 8 cut(s) 23, 203, 294, 416, 462, 566, 656, 911
HpyF3I CTNAG 3 cut(s) 165, 1300, 1343
HpySE526I ACGT 3 cut(s) 234, 311, 638
Hsp92I GRCGYC 1 cut(s) 234
HspAI GCGC 2 cut(s) 110, 903
Kpn2I TCCGGA 2 cut(s) 253, 299
Ksp22I TGATCA 2 cut(s) 997, 1036
KspAI GTTAAC 1 cut(s) 850
LmnI GCTCC 5 cut(s) 71, 233, 302, 1135, 1312
Lsp1109I GCAGC 3 cut(s) 394, 449, 802
LweI GCATC 4 cut(s) 637, 879, 901, 1294
MaeI CTAG 1 cut(s) 23
MaeII ACGT 3 cut(s) 234, 311, 638
MaeIII GTNAC 1 cut(s) 634
MbiI CCGCTC 1 cut(s) 1138
MboII GAAGA 7 cut(s) 440, 526, 608, 1001, 1053, 1160, 1195
MflI RGATCY 1 cut(s) 1093
MhlI GDGCHC 4 cut(s) 45, 76, 299, 1256
MlsI TGGCCA 1 cut(s) 1023
MluCI AATT 4 cut(s) 279, 351, 576, 1360
MluNI TGGCCA 1 cut(s) 1023
MlyI GAGTC 2 cut(s) 144, 180
MmeI TCCRAC 4 cut(s) 49, 255, 603, 1113
Mox20I TGGCCA 1 cut(s) 1023
MroI TCCGGA 2 cut(s) 253, 299
MscI TGGCCA 1 cut(s) 1023
MseI TTAA 6 cut(s) 318, 507, 609, 699, 849, 1329
MslI CAYNNNNRTG 9 cut(s) 672, 948, 981, 984, 1005, 1017, 1032, 1038, 1044
Msp20I TGGCCA 1 cut(s) 1023
MspI CCGG 5 cut(s) 77, 254, 269, 285, 300
MspR9I CCNGG 7 cut(s) 64, 77, 100, 269, 854, 1053, 1208
MvaI CCWGG 5 cut(s) 64, 100, 854, 1053, 1208
MvnI CGCG 1 cut(s) 110
MwoI GCNNNNNNNGC 8 cut(s) 23, 203, 294, 416, 462, 566, 656, 911
NciI CCSGG 2 cut(s) 77, 269
NlaIV GGNNCC 6 cut(s) 73, 229, 298, 455, 1253, 1254
NmeAIII GCCGAG 1 cut(s) 1098
NmuCI GTSAC 1 cut(s) 634
NspI RCATGY 2 cut(s) 659, 1114
PagI TCATGA 3 cut(s) 949, 982, 1216
PciI ACATGT 1 cut(s) 1110
PcsI WCGNNNNNNNCGW 1 cut(s) 231
PfeI GAWTC 1 cut(s) 247
PflMI CCANNNNNTGG 1 cut(s) 1113
PkrI GCNGC 6 cut(s) 28, 196, 216, 409, 464, 792
PleI GAGTC 2 cut(s) 144, 179
PpsI GAGTC 2 cut(s) 144, 179
PscI ACATGT 1 cut(s) 1110
Psp6I CCWGG 5 cut(s) 62, 98, 852, 1051, 1206
PspFI CCCAGC 1 cut(s) 419
PspGI CCWGG 5 cut(s) 62, 98, 852, 1051, 1206
PspN4I GGNNCC 6 cut(s) 73, 229, 298, 455, 1253, 1254
PspOMI GGGCCC 1 cut(s) 1252
PspPI GGNCC 4 cut(s) 79, 337, 1252, 1253
PstNI CAGNNNCTG 1 cut(s) 1315
PsuI RGATCY 1 cut(s) 1093
RsaI GTAC 2 cut(s) 832, 864
RsaNI GTAC 2 cut(s) 831, 863
RseI CAYNNNNRTG 9 cut(s) 672, 948, 981, 984, 1005, 1017, 1032, 1038, 1044
SaqAI TTAA 6 cut(s) 318, 507, 609, 699, 849, 1329
SatI GCNGC 6 cut(s) 27, 195, 215, 408, 463, 791
Sau96I GGNCC 4 cut(s) 79, 337, 1252, 1253
SchI GAGTC 2 cut(s) 144, 180
ScrFI CCNGG 7 cut(s) 64, 77, 100, 269, 854, 1053, 1208
SduI GDGCHC 4 cut(s) 45, 76, 299, 1256
SfaNI GCATC 4 cut(s) 637, 879, 901, 1294
SinI GGWCC 1 cut(s) 337
SmiMI CAYNNNNRTG 9 cut(s) 672, 948, 981, 984, 1005, 1017, 1032, 1038, 1044
SmlI CTYRAG 2 cut(s) 175, 1097
SmoI CTYRAG 2 cut(s) 175, 1097
Sse9I AATT 4 cut(s) 279, 351, 576, 1360
SspMI CTAG 1 cut(s) 23
StyD4I CCNGG 7 cut(s) 62, 75, 98, 267, 852, 1051, 1206
StyI CCWWGG 1 cut(s) 1228
TaaI ACNGT 3 cut(s) 469, 629, 1128
TaiI ACGT 3 cut(s) 237, 314, 641
TaqI TCGA 3 cut(s) 528, 661, 1243
TasI AATT 4 cut(s) 279, 351, 576, 1360
TatI WGTACW 1 cut(s) 862
TauI GCSGC 3 cut(s) 29, 197, 217
TfiI GAWTC 1 cut(s) 247
Tru1I TTAA 6 cut(s) 318, 507, 609, 699, 849, 1329
Tru9I TTAA 6 cut(s) 318, 507, 609, 699, 849, 1329
TscAI CASTG 3 cut(s) 208, 472, 1131
TseFI GTSAC 1 cut(s) 634
TseI GCWGC 3 cut(s) 407, 462, 790
Tsp45I GTSAC 1 cut(s) 634
TspDTI ATGAA 8 cut(s) 78, 656, 690, 1002, 1023, 1175, 1205, 1276
TspRI CASTG 3 cut(s) 208, 472, 1131
Van91I CCANNNNNTGG 1 cut(s) 1113
VpaK11BI GGWCC 1 cut(s) 337
XceI RCATGY 2 cut(s) 659, 1114
XmiI GTMKAC 1 cut(s) 384
XspI CTAG 1 cut(s) 23
ZraI GACGTC 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.