AT1G80480

Cobalamin synthesis protein cobW C-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001734
Physical Location & Seq
Reverse (-)
30258005 .. 30260732
2728 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G80480.1

Sequence Viewer

Length: 1335 bp
ATGGCGACGCTATCAACACTAGAGATTGCTACGACCTTCCTCTCTTTCACCGCTCCTCGTTCCTCTGCCGCCTTCAACTATCGCTTTTCTTCTGCCGCCGTCTCTGTTCTCTCCAGGCCCAGAGCTACCACCGTTTCCGTCAGAACGACGCCGTCTTTCTTCTATTCCCCGGTGGTCCGCCGTCAAAGATTCTCATCTGTCTCCGCCTCAGCTACGCAGACTGAAGATTCTGATGTCACCACTAAGATTCCGCCAGATAACCGGATTCCGGCTACTATTATCACCGGTTTTTTGGGCTCTGGCAAGACAACATTGCTGAATCATATACTGACCAGAGATCATGGGAAGCGAATAGCCGTGATTGAGAATGAGTTTGGTGAAGTTGACATTGATGGGTCACTTGTTGCTTCTAAATCTATTGGAGCTGAGGATATTGTGATGCTCAACAATGGCTGCCTTTGTTGCACTGTTAGGGGTGATCTTGTCAGGATGATTGGAGAATTAGTCAATACCAAGAAAGGAAAGTTCGATCATATAGTCATAGAGACTACAGGATTGGCAAACCCGGCACCGATTATCCAAACCTTTTATGCCGAGGAAGAAATTTTCAATGATGTCAAACTGGACGGTGTTGTTACTCTAGTTGATGCTAAGCATGCTCGTTTGCATCTAGACGAGGTCAAACCCGAAGGTGTTGTCAATGAGGCTGTTGAACAAATAGCTTATGCAGATCGTATCATAGTTAACAAGACTGATCTTGTTGGTGAGGCGGAATTAGGTTCGGTGGTACAACGCATAAAGACAATAAACAGCATGGCTCAGATGACGCGAACAAAGTACGGAAATGTTGACTTAGATTATGTTCTTGGAATTGGAGGTTTTGATCTAGAGAGAATCGAAAGCTCTGTGAATGAAGATGATAAGGGAGATCACCATGATCACGACCATGATCATCATCATGATCACAACCATGACCACGACCATCATCACCACGATGGACATGATCATCATCATCATTCTCATGATCACACCCATGATCCCGGTGTTTCTTCAGTCAGTATAGTTTGCGAAGGAAGCTTAGACCTTGAGAAGGCAAACATGTGGCTCGGGACGTTACTGATGGAACGCAGTGAAGACATCTACAGAATGAAAGGTCTCCTCTCGGTCCACACCATGGAGGAGAGATTCGTGTTTCAAGGAGTCCATGACATATTTCAAGGATCACCAGACAGGTTATGGGGAAGAGAGGAGGAGAGAGTGAATAAGATCGTTTTCATTGGTAAGAATTTGAACAGGGAGGAGTTAGAGAAGGGTTTTAAAGCTTGCTTGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CobW_C cobW

Protein Analysis

444

Amino Acids

49.49

Weight (kDa)

5.67

Isoelectric Point (pI)

35.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cobW PF02492 90 - 276 3.6e-60 CobW/HypB/UreG, nucleotide-binding domain
CobW_C PF07683 349 - 443 5.4e-22 Cobalamin synthesis protein cobW C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 568
AccB7I CCANNNNNTGG 1 cut(s) 1174
AccBSI CCGCTC 1 cut(s) 53
AccII CGCG 1 cut(s) 829
AciI CCGC 7 cut(s) 51, 69, 96, 178, 204, 251, 770
AclWI GGATC 2 cut(s) 1031, 1228
AcsI RAATTY 2 cut(s) 603, 1285
AcuI CTGAAG 2 cut(s) 243, 1035
AcyI GRCGYC 1 cut(s) 149
AfaI GTAC 2 cut(s) 789, 839
AfiI CCNNNNNNNGG 3 cut(s) 268, 1090, 1174
AflIII ACRYGT 1 cut(s) 1098
AgeI ACCGGT 1 cut(s) 284
AgsI TTSAA 6 cut(s) 76, 610, 713, 1196, 1217, 1291
AjnI CCWGG 1 cut(s) 113
AluBI AGCT 7 cut(s) 125, 212, 425, 722, 903, 1077, 1322
AluI AGCT 7 cut(s) 125, 212, 425, 722, 903, 1077, 1322
Alw26I GTCTC 4 cut(s) 106, 205, 539, 1160
AlwI GGATC 2 cut(s) 1031, 1228
Ama87I CYCGRG 1 cut(s) 1106
AoxI GGCC 1 cut(s) 116
ApeKI GCWGC 1 cut(s) 453
ApoI RAATTY 2 cut(s) 603, 1285
ArsI GACNNNNNNTTYG 2 cut(s) 681, 713
AsiGI ACCGGT 1 cut(s) 284
AspS9I GGNCC 3 cut(s) 117, 175, 1165
AsuC2I CCSGG 3 cut(s) 170, 566, 1041
AsuHPI GGTGA 9 cut(s) 40, 229, 274, 389, 488, 776, 923, 980, 1215
AvaI CYCGRG 1 cut(s) 1106
AvaII GGWCC 2 cut(s) 175, 1165
BanI GGYRCC 1 cut(s) 568
BanII GRGCYC 1 cut(s) 299
BbsI GAAGAC 1 cut(s) 1140
BbvCI CCTCAGC 2 cut(s) 208, 426
BbvI GCAGC 1 cut(s) 440
BccI CCATC 4 cut(s) 386, 989, 990, 1114
BceAI ACGGC 4 cut(s) 83, 136, 165, 341
BciT130I CCWGG 1 cut(s) 115
BclI TGATCA 5 cut(s) 937, 949, 961, 1003, 1024
BcnI CCSGG 3 cut(s) 170, 566, 1041
BcoDI GTCTC 4 cut(s) 106, 205, 539, 1160
BfaI CTAG 4 cut(s) 20, 641, 671, 887
BfmI CTRYAG 2 cut(s) 549, 1141
BisI GCNGC 3 cut(s) 69, 96, 454
BlpI GCTNAGC 1 cut(s) 651
BlsI GCNGC 3 cut(s) 70, 97, 455
Bme1390I CCNGG 4 cut(s) 115, 170, 566, 1041
Bme18I GGWCC 2 cut(s) 175, 1165
BmeT110I CYCGRG 1 cut(s) 1106
BmgT120I GGNCC 3 cut(s) 117, 175, 1165
BmiI GGNNCC 1 cut(s) 570
BmrFI CCNGG 4 cut(s) 115, 170, 566, 1041
BmsI GCATC 3 cut(s) 429, 637, 676
BpiI GAAGAC 1 cut(s) 1140
BpmI CTGGAG 1 cut(s) 97
Bpu10I CCTNAGC 2 cut(s) 208, 426
Bpu1102I GCTNAGC 1 cut(s) 651
BpuEI CTTGAG 1 cut(s) 1106
BpuMI CCSGG 3 cut(s) 170, 566, 1041
BsaBI GATNNNNATC 3 cut(s) 193, 954, 1008
BsaHI GRCGYC 1 cut(s) 149
BsaI GGTCTC 1 cut(s) 1160
BsaJI CCNNGG 3 cut(s) 168, 594, 1173
BsaWI WCCGGW 2 cut(s) 261, 284
BsaXI ACNNNNNCTCC 2 cut(s) 489, 519
Bsc4I CCNNNNNNNGG 3 cut(s) 268, 1090, 1174
Bse118I RCCGGY 1 cut(s) 284
Bse1I ACTGG 1 cut(s) 627
Bse3DI GCAATG 1 cut(s) 311
Bse8I GATNNNNATC 3 cut(s) 193, 954, 1008
BseBI CCWGG 1 cut(s) 115
BseDI CCNNGG 3 cut(s) 168, 594, 1173
BseGI GGATG 1 cut(s) 495
BseJI GATNNNNATC 3 cut(s) 193, 954, 1008
BseLI CCNNNNNNNGG 3 cut(s) 268, 1090, 1174
BseMI GCAATG 1 cut(s) 311
BseMII CTCAG 3 cut(s) 222, 417, 833
BseNI ACTGG 1 cut(s) 627
BseRI GAGGAG 6 cut(s) 45, 1148, 1193, 1262, 1265, 1313
BseXI GCAGC 1 cut(s) 440
Bsh1236I CGCG 1 cut(s) 829
BshFI GGCC 1 cut(s) 118
BshNI GGYRCC 1 cut(s) 568
BshTI ACCGGT 1 cut(s) 284
BsiHKCI CYCGRG 1 cut(s) 1106
BsiSI CCGG 6 cut(s) 170, 262, 269, 285, 566, 1041
BslFI GGGAC 1 cut(s) 1123
BslI CCNNNNNNNGG 3 cut(s) 268, 1090, 1174
BsmAI GTCTC 4 cut(s) 106, 205, 539, 1160
BsmBI CGTCTC 1 cut(s) 106
BsmFI GGGAC 1 cut(s) 1123
BsnI GGCC 1 cut(s) 118
Bso31I GGTCTC 1 cut(s) 1160
BsoBI CYCGRG 1 cut(s) 1106
Bsp1286I GDGCHC 1 cut(s) 299
Bsp1720I GCTNAGC 1 cut(s) 651
Bsp19I CCATGG 1 cut(s) 1173
BspACI CCGC 7 cut(s) 51, 69, 96, 178, 204, 251, 770
BspANI GGCC 1 cut(s) 118
BspCNI CTCAG 3 cut(s) 221, 418, 832
BspFNI CGCG 1 cut(s) 829
BspHI TCATGA 2 cut(s) 958, 1021
BspLI GGNNCC 1 cut(s) 570
BspPI GGATC 2 cut(s) 1031, 1228
BspT107I GGYRCC 1 cut(s) 568
BspTNI GGTCTC 1 cut(s) 1160
BsrBI CCGCTC 1 cut(s) 53
BsrDI GCAATG 1 cut(s) 311
BsrFI RCCGGY 1 cut(s) 284
BsrI ACTGG 1 cut(s) 627
BssAI RCCGGY 1 cut(s) 284
BssECI CCNNGG 3 cut(s) 168, 594, 1173
BssNI GRCGYC 1 cut(s) 149
BssT1I CCWWGG 1 cut(s) 1173
Bst2UI CCWGG 1 cut(s) 115
Bst4CI ACNGT 3 cut(s) 133, 469, 629
Bst6I CTCTTC 1 cut(s) 1237
BstACI GRCGYC 1 cut(s) 149
BstC8I GCNNGC 2 cut(s) 657, 1324
BstDEI CTNAG 7 cut(s) 208, 243, 426, 651, 819, 853, 1078
BstDSI CCRYGG 1 cut(s) 1173
BstENI CCTNNNNNAGG 1 cut(s) 1088
BstF5I GGATG 1 cut(s) 495
BstFNI CGCG 1 cut(s) 829
BstMAI GTCTC 4 cut(s) 106, 205, 539, 1160
BstMWI GCNNNNNNNGC 4 cut(s) 462, 566, 656, 1074
BstNI CCWGG 1 cut(s) 115
BstNSI RCATGY 2 cut(s) 659, 1102
BstSCI CCNGG 4 cut(s) 113, 168, 564, 1039
BstSFI CTRYAG 2 cut(s) 549, 1141
BstUI CGCG 1 cut(s) 829
BstV1I GCAGC 1 cut(s) 440
BstV2I GAAGAC 1 cut(s) 1140
BsuRI GGCC 1 cut(s) 118
BtgI CCRYGG 1 cut(s) 1173
BtsCI GGATG 1 cut(s) 495
BtsI GCAGTG 1 cut(s) 1135
BtsIMutI CAGTG 2 cut(s) 465, 1135
Cac8I GCNNGC 2 cut(s) 657, 1324
CciI TCATGA 2 cut(s) 958, 1021
Cfr10I RCCGGY 1 cut(s) 284
Cfr13I GGNCC 3 cut(s) 117, 175, 1165
CseI GACGC 3 cut(s) 16, 157, 835
Csp6I GTAC 2 cut(s) 788, 838
CspAI ACCGGT 1 cut(s) 284
CviQI GTAC 2 cut(s) 788, 838
DdeI CTNAG 7 cut(s) 208, 243, 426, 651, 819, 853, 1078
DraI TTTAAA 1 cut(s) 1318
Eam1104I CTCTTC 1 cut(s) 1237
EarI CTCTTC 1 cut(s) 1237
EciI GGCGGA 4 cut(s) 167, 193, 240, 785
Eco130I CCWWGG 1 cut(s) 1173
Eco24I GRGCYC 1 cut(s) 299
Eco31I GGTCTC 1 cut(s) 1160
Eco47I GGWCC 2 cut(s) 175, 1165
Eco57I CTGAAG 2 cut(s) 243, 1035
Eco88I CYCGRG 1 cut(s) 1106
EcoNI CCTNNNNNAGG 1 cut(s) 1088
EcoRII CCWGG 1 cut(s) 113
EcoT14I CCWWGG 1 cut(s) 1173
EcoT38I GRGCYC 1 cut(s) 299
ErhI CCWWGG 1 cut(s) 1173
Esp3I CGTCTC 1 cut(s) 106
FaqI GGGAC 1 cut(s) 1123
FbaI TGATCA 5 cut(s) 937, 949, 961, 1003, 1024
Fnu4HI GCNGC 3 cut(s) 69, 96, 454
FokI GGATG 1 cut(s) 502
FriOI GRGCYC 1 cut(s) 299
Fsp4HI GCNGC 3 cut(s) 69, 96, 454
FspBI CTAG 4 cut(s) 20, 641, 671, 887
GluI GCNGC 3 cut(s) 69, 96, 454
GsuI CTGGAG 1 cut(s) 97
HaeIII GGCC 1 cut(s) 118
HapII CCGG 6 cut(s) 170, 262, 269, 285, 566, 1041
HgaI GACGC 3 cut(s) 16, 157, 835
Hin1I GRCGYC 1 cut(s) 149
HincII GTYRAC 3 cut(s) 385, 745, 850
HindII GTYRAC 3 cut(s) 385, 745, 850
HindIII AAGCTT 2 cut(s) 1075, 1320
HinfI GANTC 8 cut(s) 189, 227, 247, 265, 319, 894, 1185, 1200
HpaI GTTAAC 1 cut(s) 745
HpaII CCGG 6 cut(s) 170, 262, 269, 285, 566, 1041
HphI GGTGA 9 cut(s) 40, 229, 274, 389, 488, 776, 923, 980, 1215
Hpy166II GTNNAC 4 cut(s) 385, 745, 850, 1168
Hpy188I TCNGA 3 cut(s) 143, 232, 822
Hpy188III TCNNGA 7 cut(s) 487, 671, 887, 941, 959, 1022, 1108
Hpy8I GTNNAC 4 cut(s) 385, 745, 850, 1168
Hpy99I CGWCG 2 cut(s) 10, 151
HpyAV CCTTC 6 cut(s) 46, 82, 683, 1064, 1084, 1303
HpyCH4III ACNGT 3 cut(s) 133, 469, 629
HpyCH4IV ACGT 1 cut(s) 1112
HpyCH4V TGCA 3 cut(s) 465, 667, 728
HpyF10VI GCNNNNNNNGC 4 cut(s) 462, 566, 656, 1074
HpyF3I CTNAG 7 cut(s) 208, 243, 426, 651, 819, 853, 1078
HpySE526I ACGT 1 cut(s) 1112
Hsp92I GRCGYC 1 cut(s) 149
Ksp22I TGATCA 5 cut(s) 937, 949, 961, 1003, 1024
KspAI GTTAAC 1 cut(s) 745
LmnI GCTCC 2 cut(s) 58, 422
Lsp1109I GCAGC 1 cut(s) 440
LweI GCATC 3 cut(s) 429, 637, 676
MaeI CTAG 4 cut(s) 20, 641, 671, 887
MaeII ACGT 1 cut(s) 1112
MaeIII GTNAC 4 cut(s) 235, 396, 634, 1113
MbiI CCGCTC 1 cut(s) 53
MboII GAAGA 8 cut(s) 81, 151, 236, 611, 926, 1041, 1145, 1254
MhlI GDGCHC 1 cut(s) 299
MluCI AATT 5 cut(s) 500, 603, 773, 870, 1285
MlyI GAGTC 1 cut(s) 1209
MseI TTAA 2 cut(s) 744, 1317
MslI CAYNNNNRTG 6 cut(s) 945, 957, 969, 993, 1020, 1032
MspI CCGG 6 cut(s) 170, 262, 269, 285, 566, 1041
MspR9I CCNGG 4 cut(s) 115, 170, 566, 1041
MvaI CCWGG 1 cut(s) 115
MvnI CGCG 1 cut(s) 829
MwoI GCNNNNNNNGC 4 cut(s) 462, 566, 656, 1074
NciI CCSGG 3 cut(s) 170, 566, 1041
NcoI CCATGG 1 cut(s) 1173
NlaIV GGNNCC 1 cut(s) 570
NmeAIII GCCGAG 1 cut(s) 619
NmuCI GTSAC 2 cut(s) 235, 396
NspI RCATGY 2 cut(s) 659, 1102
PaeI GCATGC 1 cut(s) 659
PagI TCATGA 2 cut(s) 958, 1021
PciI ACATGT 1 cut(s) 1098
PfeI GAWTC 7 cut(s) 189, 227, 247, 265, 319, 894, 1185
PflFI GACNNNGTC 2 cut(s) 151, 677
PflMI CCANNNNNTGG 1 cut(s) 1174
PinAI ACCGGT 1 cut(s) 284
PkrI GCNGC 3 cut(s) 70, 97, 455
PleI GAGTC 1 cut(s) 1208
PpsI GAGTC 1 cut(s) 1208
PscI ACATGT 1 cut(s) 1098
Psp6I CCWGG 1 cut(s) 113
PspGI CCWGG 1 cut(s) 113
PspN4I GGNNCC 1 cut(s) 570
PspPI GGNCC 3 cut(s) 117, 175, 1165
PsyI GACNNNGTC 2 cut(s) 151, 677
RsaI GTAC 2 cut(s) 789, 839
RsaNI GTAC 2 cut(s) 788, 838
RseI CAYNNNNRTG 6 cut(s) 945, 957, 969, 993, 1020, 1032
SaqAI TTAA 2 cut(s) 744, 1317
SatI GCNGC 3 cut(s) 69, 96, 454
Sau96I GGNCC 3 cut(s) 117, 175, 1165
SchI GAGTC 1 cut(s) 1209
ScrFI CCNGG 4 cut(s) 115, 170, 566, 1041
SduI GDGCHC 1 cut(s) 299
SfaNI GCATC 3 cut(s) 429, 637, 676
SfcI CTRYAG 2 cut(s) 549, 1141
SinI GGWCC 2 cut(s) 175, 1165
SmiMI CAYNNNNRTG 6 cut(s) 945, 957, 969, 993, 1020, 1032
SmlI CTYRAG 1 cut(s) 1085
SmoI CTYRAG 1 cut(s) 1085
SphI GCATGC 1 cut(s) 659
Sse9I AATT 5 cut(s) 500, 603, 773, 870, 1285
SsiI CCGC 7 cut(s) 51, 69, 96, 178, 204, 251, 770
SspMI CTAG 4 cut(s) 20, 641, 671, 887
StyD4I CCNGG 4 cut(s) 113, 168, 564, 1039
StyI CCWWGG 1 cut(s) 1173
TaaI ACNGT 3 cut(s) 133, 469, 629
TaiI ACGT 1 cut(s) 1115
TaqI TCGA 2 cut(s) 528, 897
TaqII GACCGA 1 cut(s) 1153
TasI AATT 5 cut(s) 500, 603, 773, 870, 1285
TauI GCSGC 2 cut(s) 71, 98
TfiI GAWTC 7 cut(s) 189, 227, 247, 265, 319, 894, 1185
Tru1I TTAA 2 cut(s) 744, 1317
Tru9I TTAA 2 cut(s) 744, 1317
TscAI CASTG 2 cut(s) 472, 1135
TseFI GTSAC 2 cut(s) 235, 396
TseI GCWGC 1 cut(s) 453
Tsp45I GTSAC 2 cut(s) 235, 396
TspDTI ATGAA 3 cut(s) 927, 1163, 1264
TspGWI ACGGA 2 cut(s) 127, 855
TspRI CASTG 2 cut(s) 472, 1135
Tth111I GACNNNGTC 2 cut(s) 151, 677
Van91I CCANNNNNTGG 1 cut(s) 1174
VpaK11BI GGWCC 2 cut(s) 175, 1165
XagI CCTNNNNNAGG 1 cut(s) 1088
XapI RAATTY 2 cut(s) 603, 1285
XbaI TCTAGA 2 cut(s) 670, 886
XceI RCATGY 2 cut(s) 659, 1102
XcmI CCANNNNNNNNNTGG 1 cut(s) 1233
XspI CTAG 4 cut(s) 20, 641, 671, 887
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.