RchiOBHm_Chr2g0134411

COBW domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001734
Physical Location & Seq
Forward (+)
51548421 .. 51554169
5749 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50545

Sequence Viewer

Length: 1365 bp
ATGGCTTCCATATCGGCCGACATAGCCGCCACCCTTCTCTCCCTAACTTCCCGTCACTCCATCCGGGTCGGAGCCCCGGCCCTATTCCCTCTCACATGGAGACCCCGGACTTCCCCACTGTCCCATACCCTCAGCTACTCCACTTCAACTTCCTCCTTTCGCCAGAGACTCGTCTCCAACTCTAAGCGGGGCTCGAGAAGGTTCACTGTGGCCGCCACCACTGCCACGCCACCGCAGAGTGACAGCTCCGACGTTTTGACCCAGATTCCGCCGGATAATCGTATCCCGGCGACCATAATTACCGGCTTCTTGGGCTCCGGAAAGACTACATTGCTTAATCATATATTGACTGCCGACCACGGGAAGCGAATTGCAGTGATTGAAAATGAGTATGGTGAAGTAGACATTGATGGTTCTTTGGTGGCTGCGAAATCCGCTGGGGCCGAAGATATTGTTATGCTGAATAATGGGTGCCTTTGCTGCACTGTGAGGGGTGATCTTGTGCGGATGATTTCAGACTTGGTTAATAAGAAGAAAGGGAGGTTTGATCATATTGTGATAGAGACTACAGGATTGGCAAATCCAGCACCGATAATTCAAACATTTTATGCGGAAGATCAGATTTTCAATGATGTCAAGTTGGACGGTGTTGTCACGTTAGTTGATGCTAAACATGCTCGACTTCATCTGGATGAAGTCAAGGCAGAAGGAGTGGTTAATGAGGCAGTAGAGCAGATTGCTTATGCTGATCGTATCATTGTGAATAAGACTGATCTTGTTGGCGAGTCAGATATTGCTTCTTTGGTGCAGAGAATAAGGAACATTAATGGCATGGCTCAACTGAAGCGTACAGAGTTTGGAAAAGTTAACCTGGATTATGTGCTTGGTATCGGAGGATTTGATTTGGAGAGGATAGAGAGTGCTATTGATGCTGAAGCTGAGAAGGAAGATCATGACCATCATCACGACCACCATGACCATCATCACCATCATCACCATCATGATGAAGAACATGATCACAAACATGAACATCATGATGGTCACCACTCCCATGATCATGTTCATGATCCTGGTGTTTCTTCTGTCAGTATTGTTTGCGAAGGGAACCTCGATCTGGAGAAGGCCAACATGTGGCTTGGCACACTGTTGTTGGACCGGAGTGAGGACATATATCGTATGAAAGGTCTTCTATCAGTTCAGGGTATGGATGAGAGATTTGTCTTCCAGGGAGTTCATGATATCTTCCAAGGTTCTCCCGATCGGTTGTGGGGCCCCAATGAGCCAAGAACAAATAAGATTGTTTTCATAGGGAAGAACTTGGATGCACAAGAGTTGGAGAAGGGTTTTAAAGGCTGTTTACTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CobW_C cobW

Protein Analysis

454

Amino Acids

50.27

Weight (kDa)

5.89

Isoelectric Point (pI)

32.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cobW PF02492 96 - 280 2.1e-59 CobW/HypB/UreG, nucleotide-binding domain
CobW_C PF07683 359 - 453 9.6e-23 Cobalamin synthesis protein cobW C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 650
AccB1I GGYRCC 1 cut(s) 471
AccB7I CCANNNNNTGG 1 cut(s) 1131
AccI GTMKAC 1 cut(s) 402
AccIII TCCGGA 1 cut(s) 317
AciI CCGC 8 cut(s) 27, 187, 213, 233, 269, 435, 505, 611
AclWI GGATC 1 cut(s) 1061
AcoI YGGCCR 2 cut(s) 15, 210
AcuI CTGAAG 2 cut(s) 863, 954
AfaI GTAC 1 cut(s) 850
AfiI CCNNNNNNNGG 4 cut(s) 360, 1114, 1131, 1162
AflIII ACRYGT 1 cut(s) 1128
AgsI TTSAA 4 cut(s) 147, 383, 599, 628
AjnI CCWGG 3 cut(s) 870, 1069, 1224
AluBI AGCT 3 cut(s) 135, 246, 938
AluI AGCT 3 cut(s) 135, 246, 938
Alw26I GTCTC 4 cut(s) 94, 160, 178, 557
AlwI GGATC 1 cut(s) 1061
Ama87I CYCGRG 1 cut(s) 193
Aor13HI TCCGGA 1 cut(s) 317
AoxI GGCC 6 cut(s) 15, 78, 210, 441, 1122, 1270
ApaI GGGCCC 1 cut(s) 1274
ApeKI GCWGC 2 cut(s) 425, 480
AseI ATTAAT 1 cut(s) 825
AspS9I GGNCC 5 cut(s) 79, 441, 1153, 1270, 1271
AsuC2I CCSGG 4 cut(s) 65, 77, 106, 287
AsuHPI GGTGA 5 cut(s) 407, 506, 977, 986, 1034
AvaI CYCGRG 1 cut(s) 193
AvaII GGWCC 1 cut(s) 1153
BaeGI GKGCMC 1 cut(s) 1274
BanI GGYRCC 1 cut(s) 471
BanII GRGCYC 4 cut(s) 76, 194, 317, 1274
BbsI GAAGAC 2 cut(s) 1178, 1213
BbvCI CCTCAGC 1 cut(s) 131
BbvI GCAGC 2 cut(s) 412, 467
BccI CCATC 7 cut(s) 68, 404, 966, 987, 996, 1005, 1031
BciT130I CCWGG 3 cut(s) 872, 1071, 1226
BciVI GTATCC 1 cut(s) 293
BclI TGATCA 3 cut(s) 547, 1015, 1054
BcnI CCSGG 4 cut(s) 65, 77, 106, 287
BcoDI GTCTC 4 cut(s) 94, 160, 178, 557
BfmI CTRYAG 1 cut(s) 567
BfuI GTATCC 1 cut(s) 293
BisI GCNGC 4 cut(s) 27, 213, 426, 481
BlsI GCNGC 4 cut(s) 28, 214, 427, 482
Bme1390I CCNGG 7 cut(s) 65, 77, 106, 287, 872, 1071, 1226
Bme18I GGWCC 1 cut(s) 1153
BmeT110I CYCGRG 1 cut(s) 193
BmgT120I GGNCC 5 cut(s) 79, 441, 1153, 1270, 1271
BmiI GGNNCC 8 cut(s) 73, 316, 442, 473, 1106, 1271, 1272, 1273
BmrFI CCNGG 7 cut(s) 65, 77, 106, 287, 872, 1071, 1226
BmsI GCATC 3 cut(s) 655, 919, 1312
BpiI GAAGAC 2 cut(s) 1178, 1213
BpmI CTGGAG 1 cut(s) 1136
Bpu10I CCTNAGC 1 cut(s) 131
BpuMI CCSGG 4 cut(s) 65, 77, 106, 287
BsaI GGTCTC 1 cut(s) 94
BsaJI CCNNGG 5 cut(s) 75, 104, 358, 1225, 1246
BsaWI WCCGGW 2 cut(s) 317, 1155
Bsc4I CCNNNNNNNGG 4 cut(s) 360, 1114, 1131, 1162
Bse118I RCCGGY 1 cut(s) 302
Bse3DI GCAATG 1 cut(s) 329
BseAI TCCGGA 1 cut(s) 317
BseBI CCWGG 3 cut(s) 872, 1071, 1226
BseDI CCNNGG 5 cut(s) 75, 104, 358, 1225, 1246
BseGI GGATG 5 cut(s) 60, 513, 697, 1213, 1327
BseLI CCNNNNNNNGG 4 cut(s) 360, 1114, 1131, 1162
BseMI GCAATG 1 cut(s) 329
BseMII CTCAG 2 cut(s) 145, 930
BseSI GKGCMC 1 cut(s) 1274
BseX3I CGGCCG 1 cut(s) 15
BseXI GCAGC 2 cut(s) 412, 467
BseYI CCCAGC 1 cut(s) 437
BsgI GTGCAG 2 cut(s) 466, 827
Bsh1285I CGRYCG 2 cut(s) 18, 1261
BshFI GGCC 6 cut(s) 17, 80, 212, 443, 1124, 1272
BshNI GGYRCC 1 cut(s) 471
BsiEI CGRYCG 2 cut(s) 18, 1261
BsiHKCI CYCGRG 1 cut(s) 193
BsiSI CCGG 8 cut(s) 64, 77, 106, 272, 287, 303, 318, 1156
BslFI GGGAC 1 cut(s) 106
BslI CCNNNNNNNGG 4 cut(s) 360, 1114, 1131, 1162
BsmAI GTCTC 4 cut(s) 94, 160, 178, 557
BsmBI CGTCTC 1 cut(s) 178
BsmFI GGGAC 1 cut(s) 106
BsnI GGCC 6 cut(s) 17, 80, 212, 443, 1124, 1272
Bso31I GGTCTC 1 cut(s) 94
BsoBI CYCGRG 1 cut(s) 193
Bsp120I GGGCCC 1 cut(s) 1270
Bsp1286I GDGCHC 4 cut(s) 76, 194, 317, 1274
Bsp13I TCCGGA 1 cut(s) 317
BspACI CCGC 8 cut(s) 27, 187, 213, 233, 269, 435, 505, 611
BspANI GGCC 6 cut(s) 17, 80, 212, 443, 1124, 1272
BspCNI CTCAG 2 cut(s) 144, 931
BspEI TCCGGA 1 cut(s) 317
BspHI TCATGA 5 cut(s) 952, 1000, 1033, 1063, 1234
BspLI GGNNCC 8 cut(s) 73, 316, 442, 473, 1106, 1271, 1272, 1273
BspPI GGATC 1 cut(s) 1061
BspT107I GGYRCC 1 cut(s) 471
BspTNI GGTCTC 1 cut(s) 94
BsrDI GCAATG 1 cut(s) 329
BsrFI RCCGGY 1 cut(s) 302
BssAI RCCGGY 1 cut(s) 302
BssECI CCNNGG 5 cut(s) 75, 104, 358, 1225, 1246
BssT1I CCWWGG 1 cut(s) 1246
Bst2UI CCWGG 3 cut(s) 872, 1071, 1226
Bst4CI ACNGT 5 cut(s) 120, 208, 487, 647, 1146
BstDEI CTNAG 3 cut(s) 131, 183, 939
BstDSI CCRYGG 1 cut(s) 358
BstEII GGTNACC 1 cut(s) 1040
BstF5I GGATG 5 cut(s) 60, 513, 697, 1213, 1327
BstMAI GTCTC 4 cut(s) 94, 160, 178, 557
BstMCI CGRYCG 2 cut(s) 18, 1261
BstMWI GCNNNNNNNGC 8 cut(s) 23, 221, 312, 434, 480, 584, 674, 929
BstNI CCWGG 3 cut(s) 872, 1071, 1226
BstNSI RCATGY 2 cut(s) 677, 1132
BstPI GGTNACC 1 cut(s) 1040
BstSCI CCNGG 7 cut(s) 63, 75, 104, 285, 870, 1069, 1224
BstSFI CTRYAG 1 cut(s) 567
BstSLI GKGCMC 1 cut(s) 1274
BstV1I GCAGC 2 cut(s) 412, 467
BstV2I GAAGAC 2 cut(s) 1178, 1213
BstZI CGGCCG 1 cut(s) 15
BsuI GTATCC 1 cut(s) 293
BsuRI GGCC 6 cut(s) 17, 80, 212, 443, 1124, 1272
BtgI CCRYGG 1 cut(s) 358
BtsCI GGATG 5 cut(s) 60, 513, 697, 1213, 1327
BtsI GCAGTG 2 cut(s) 219, 381
BtsIMutI CAGTG 6 cut(s) 116, 204, 219, 381, 483, 1142
CciI TCATGA 5 cut(s) 952, 1000, 1033, 1063, 1234
Cfr10I RCCGGY 1 cut(s) 302
Cfr13I GGNCC 5 cut(s) 79, 441, 1153, 1270, 1271
Csp6I GTAC 1 cut(s) 849
CviQI GTAC 1 cut(s) 849
DdeI CTNAG 3 cut(s) 131, 183, 939
DraI TTTAAA 1 cut(s) 1348
DrdI GACNNNNNNGTC 1 cut(s) 650
DseDI GACNNNNNNGTC 1 cut(s) 650
EaeI YGGCCR 2 cut(s) 15, 210
EagI CGGCCG 1 cut(s) 15
EciI GGCGGA 1 cut(s) 258
EclXI CGGCCG 1 cut(s) 15
Eco130I CCWWGG 1 cut(s) 1246
Eco24I GRGCYC 4 cut(s) 76, 194, 317, 1274
Eco31I GGTCTC 1 cut(s) 94
Eco32I GATATC 1 cut(s) 1240
Eco47I GGWCC 1 cut(s) 1153
Eco52I CGGCCG 1 cut(s) 15
Eco57I CTGAAG 2 cut(s) 863, 954
Eco88I CYCGRG 1 cut(s) 193
Eco91I GGTNACC 1 cut(s) 1040
EcoO109I RGGNCCY 2 cut(s) 1270, 1271
EcoO65I GGTNACC 1 cut(s) 1040
EcoRII CCWGG 3 cut(s) 870, 1069, 1224
EcoRV GATATC 1 cut(s) 1240
EcoT14I CCWWGG 1 cut(s) 1246
EcoT38I GRGCYC 4 cut(s) 76, 194, 317, 1274
ErhI CCWWGG 1 cut(s) 1246
Esp3I CGTCTC 1 cut(s) 178
FaqI GGGAC 1 cut(s) 106
FauI CCCGC 1 cut(s) 180
FbaI TGATCA 3 cut(s) 547, 1015, 1054
FblI GTMKAC 1 cut(s) 402
Fnu4HI GCNGC 4 cut(s) 27, 213, 426, 481
FokI GGATG 5 cut(s) 47, 520, 704, 1220, 1334
FriOI GRGCYC 4 cut(s) 76, 194, 317, 1274
Fsp4HI GCNGC 4 cut(s) 27, 213, 426, 481
GluI GCNGC 4 cut(s) 27, 213, 426, 481
GsaI CCCAGC 1 cut(s) 441
GsuI CTGGAG 1 cut(s) 1136
HaeIII GGCC 6 cut(s) 17, 80, 212, 443, 1124, 1272
HapII CCGG 8 cut(s) 64, 77, 106, 272, 287, 303, 318, 1156
HincII GTYRAC 1 cut(s) 868
HindII GTYRAC 1 cut(s) 868
HinfI GANTC 3 cut(s) 168, 265, 785
HpaI GTTAAC 1 cut(s) 868
HpaII CCGG 8 cut(s) 64, 77, 106, 272, 287, 303, 318, 1156
HphI GGTGA 5 cut(s) 407, 506, 977, 986, 1034
Hpy166II GTNNAC 4 cut(s) 204, 403, 868, 1358
Hpy188I TCNGA 6 cut(s) 71, 250, 517, 621, 790, 893
Hpy8I GTNNAC 4 cut(s) 204, 403, 868, 1358
Hpy99I CGWCG 1 cut(s) 254
HpyAV CCTTC 7 cut(s) 44, 192, 701, 937, 1094, 1114, 1333
HpyCH4III ACNGT 5 cut(s) 120, 208, 487, 647, 1146
HpyCH4IV ACGT 2 cut(s) 252, 656
HpyCH4V TGCA 4 cut(s) 374, 483, 808, 1325
HpyF10VI GCNNNNNNNGC 8 cut(s) 23, 221, 312, 434, 480, 584, 674, 929
HpyF3I CTNAG 3 cut(s) 131, 183, 939
HpySE526I ACGT 2 cut(s) 252, 656
Kpn2I TCCGGA 1 cut(s) 317
Ksp22I TGATCA 3 cut(s) 547, 1015, 1054
KspAI GTTAAC 1 cut(s) 868
LmnI GCTCC 3 cut(s) 71, 251, 320
Lsp1109I GCAGC 2 cut(s) 412, 467
LweI GCATC 3 cut(s) 655, 919, 1312
MaeII ACGT 2 cut(s) 252, 656
MaeIII GTNAC 4 cut(s) 53, 239, 652, 1040
MhlI GDGCHC 4 cut(s) 76, 194, 317, 1274
MluCI AATT 3 cut(s) 297, 369, 594
MlyI GAGTC 2 cut(s) 162, 794
MmeI TCCRAC 6 cut(s) 49, 201, 273, 621, 1131, 1314
MroI TCCGGA 1 cut(s) 317
MseI TTAA 6 cut(s) 336, 525, 717, 825, 867, 1347
MslI CAYNNNNRTG 8 cut(s) 690, 999, 1002, 1023, 1035, 1050, 1056, 1062
MspA1I CMGCKG 1 cut(s) 437
MspI CCGG 8 cut(s) 64, 77, 106, 272, 287, 303, 318, 1156
MspR9I CCNGG 7 cut(s) 65, 77, 106, 287, 872, 1071, 1226
MvaI CCWGG 3 cut(s) 872, 1071, 1226
MwoI GCNNNNNNNGC 8 cut(s) 23, 221, 312, 434, 480, 584, 674, 929
NciI CCSGG 4 cut(s) 65, 77, 106, 287
NlaIV GGNNCC 8 cut(s) 73, 316, 442, 473, 1106, 1271, 1272, 1273
NmuCI GTSAC 4 cut(s) 53, 239, 652, 1040
NspI RCATGY 2 cut(s) 677, 1132
PaeR7I CTCGAG 1 cut(s) 193
PagI TCATGA 5 cut(s) 952, 1000, 1033, 1063, 1234
PciI ACATGT 1 cut(s) 1128
PfeI GAWTC 1 cut(s) 265
PflMI CCANNNNNTGG 1 cut(s) 1131
PkrI GCNGC 4 cut(s) 28, 214, 427, 482
Ple19I CGATCG 1 cut(s) 1261
PleI GAGTC 2 cut(s) 162, 793
PpsI GAGTC 2 cut(s) 162, 793
PscI ACATGT 1 cut(s) 1128
PshBI ATTAAT 1 cut(s) 825
Psp6I CCWGG 3 cut(s) 870, 1069, 1224
PspEI GGTNACC 1 cut(s) 1040
PspFI CCCAGC 1 cut(s) 437
PspGI CCWGG 3 cut(s) 870, 1069, 1224
PspN4I GGNNCC 8 cut(s) 73, 316, 442, 473, 1106, 1271, 1272, 1273
PspOMI GGGCCC 1 cut(s) 1270
PspPI GGNCC 5 cut(s) 79, 441, 1153, 1270, 1271
PvuI CGATCG 1 cut(s) 1261
RsaI GTAC 1 cut(s) 850
RsaNI GTAC 1 cut(s) 849
RseI CAYNNNNRTG 8 cut(s) 690, 999, 1002, 1023, 1035, 1050, 1056, 1062
SaqAI TTAA 6 cut(s) 336, 525, 717, 825, 867, 1347
SatI GCNGC 4 cut(s) 27, 213, 426, 481
Sau96I GGNCC 5 cut(s) 79, 441, 1153, 1270, 1271
SchI GAGTC 2 cut(s) 162, 794
ScrFI CCNGG 7 cut(s) 65, 77, 106, 287, 872, 1071, 1226
SduI GDGCHC 4 cut(s) 76, 194, 317, 1274
SfaNI GCATC 3 cut(s) 655, 919, 1312
SfcI CTRYAG 1 cut(s) 567
Sfr274I CTCGAG 1 cut(s) 193
SinI GGWCC 1 cut(s) 1153
SlaI CTCGAG 1 cut(s) 193
SmiMI CAYNNNNRTG 8 cut(s) 690, 999, 1002, 1023, 1035, 1050, 1056, 1062
SmlI CTYRAG 1 cut(s) 193
SmoI CTYRAG 1 cut(s) 193
Sse9I AATT 3 cut(s) 297, 369, 594
SsiI CCGC 8 cut(s) 27, 187, 213, 233, 269, 435, 505, 611
StyD4I CCNGG 7 cut(s) 63, 75, 104, 285, 870, 1069, 1224
StyI CCWWGG 1 cut(s) 1246
TaaI ACNGT 5 cut(s) 120, 208, 487, 647, 1146
TaiI ACGT 2 cut(s) 255, 659
TaqI TCGA 3 cut(s) 194, 679, 1110
TasI AATT 3 cut(s) 297, 369, 594
TauI GCSGC 2 cut(s) 29, 215
TfiI GAWTC 1 cut(s) 265
Tru1I TTAA 6 cut(s) 336, 525, 717, 825, 867, 1347
Tru9I TTAA 6 cut(s) 336, 525, 717, 825, 867, 1347
TscAI CASTG 6 cut(s) 123, 211, 226, 381, 490, 1149
TseFI GTSAC 4 cut(s) 53, 239, 652, 1040
TseI GCWGC 2 cut(s) 425, 480
Tsp45I GTSAC 4 cut(s) 53, 239, 652, 1040
TspDTI ATGAA 8 cut(s) 674, 708, 1020, 1041, 1052, 1193, 1223, 1294
TspRI CASTG 6 cut(s) 123, 211, 226, 381, 490, 1149
Van91I CCANNNNNTGG 1 cut(s) 1131
VpaK11BI GGWCC 1 cut(s) 1153
VspI ATTAAT 1 cut(s) 825
XceI RCATGY 2 cut(s) 677, 1132
XhoI CTCGAG 1 cut(s) 193
XmiI GTMKAC 1 cut(s) 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.