Prupe.5G023800_v2.0.a1

COBW domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0001734
Physical Location & Seq
Reverse (-)
2659951 .. 2667866
7916 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G023800.1

Sequence Viewer

Length: 1260 bp
ATGATGGCTACGAGATTCATACTCTCAAGAGCAAGAACATCAACTAAAAATCTCAATTTCCATGGTTTCTCACCACTCTCTCAATTCATCAGAAACCTCTCCTCCGTCGTTTCCCGAGAATCCAATTTCCGCAACCTCAAGAATCCTCGTCCCGGATATTCCAGGAGCATGGTCTCTGCTTCAAACTACAATCTCGACCAGGACTCGGCCTTGAGTCTCGATACTCGTGTTCCAGCCACCGTCATCACTGGCTTTCTTGGTTCTGGAAAGACTACACTGCTGAATCATATTCTGACATCTCAACATGGGAAGCGGATTGCGGTCATAGAAAATGAGTTTGGTGAGGTGGATATTGATGGATCATTGGTTGCTAGTCATTCATCTGTGAGTGAAGACATTGTCATGGTCAATAATGGTTGCCTATGCTGTACTGTTCGGGGTGATCTAGTTAAAATGCTGTTGGAGTTGGTTAAGAAAAAACGAGACAAGTTTGATCATATTGTTATAGAAACCACGGGCCTTGCTAACCCAGCTCCCGTTATAGAAACATTTTGTATGGATGAACTTGTTTCAAGACATGTGAAACTGGATGGGGTTGTTACTTTGGTTGATGCCAAGCATGCAATGCAACATTTGAACGAAGTAAAACCAAGATTTGTGGTGAATGAGGCAGTGGAACAAGTGGCATATGCTGATCGTATTATATTGAACAAGATAGATTTGGTGACCGAGACTGAATTGGAGGTGTTGACTAACAGAATCAAGCATATCAATGGGATGGCGCAAATCAAGCTAGCTAAATTTGGATCTGTGGACATGGACTTTGTTTTGGGTGTAGGAGGATATGATCTCGAAAGGATCGATAGTCAAGTTCAAGTAGATAGTTCCTGTTCTCCATCCCATCACCGTGAAGCTGGACATGGTGCCGAACACCACAATCATCATCATGACCATGTACATGATTCTACCGTCTCAAGTGTCAGCATAGTTTATGAGGGAACCCTTGATCTTGACGAGGTGGATGATTGGCTTGAAAGGTTGATCGAAGAAAAAGGAGAAGACTTGTACAGGATGAAGGGTGTCTTATCTATAAATGGTTCTGATCAACGCTATGTTTTCCAGGGGGTGCACTCCACATTGGATGGGTGCCCAGGCAGTACATGGGGACCTGATGAGAAGAGGATTAACAAGCTTGTGTTCATAGGAAGGAATTTGGATGAAACTACTCTTAGAAAAGGTTTTAGAGGTTGTCTAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CobW_C cobW

Protein Analysis

420

Amino Acids

46.64

Weight (kDa)

6.05

Isoelectric Point (pI)

34.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 923, 1146
AciI CCGC 3 cut(s) 130, 313, 320
AclWI GGATC 3 cut(s) 367, 814, 866
AcsI RAATTY 2 cut(s) 800, 1210
AfaI GTAC 4 cut(s) 430, 957, 1067, 1159
AfiI CCNNNNNNNGG 2 cut(s) 152, 205
AflIII ACRYGT 1 cut(s) 577
AgsI TTSAA 6 cut(s) 183, 573, 637, 709, 875, 1034
AjnI CCWGG 4 cut(s) 161, 198, 1119, 1150
AjuI GAANNNNNNNTTGG 2 cut(s) 321, 353
AluBI AGCT 5 cut(s) 533, 793, 797, 914, 1192
AluI AGCT 5 cut(s) 533, 793, 797, 914, 1192
Alw21I GWGCWC 1 cut(s) 1131
Alw26I GTCTC 5 cut(s) 178, 221, 477, 725, 976
Alw44I GTGCAC 1 cut(s) 1127
AlwI GGATC 3 cut(s) 367, 814, 866
Ama87I CYCGRG 1 cut(s) 114
AoxI GGCC 2 cut(s) 207, 517
ApaLI GTGCAC 1 cut(s) 1127
ApoI RAATTY 2 cut(s) 800, 1210
ArsI GACNNNNNNTTYG 2 cut(s) 806, 838
AspLEI GCGC 1 cut(s) 784
AspS9I GGNCC 2 cut(s) 517, 1166
AsuC2I CCSGG 1 cut(s) 153
AsuHPI GGTGA 6 cut(s) 63, 353, 452, 673, 736, 896
AsuNHI GCTAGC 1 cut(s) 793
AvaI CYCGRG 1 cut(s) 114
AvaII GGWCC 1 cut(s) 1166
BaeGI GKGCMC 2 cut(s) 1131, 1151
BaeI ACNNNNGTAYC 2 cut(s) 213, 246
BanI GGYRCC 2 cut(s) 923, 1146
BauI CACGAG 1 cut(s) 225
BbsI GAAGAC 2 cut(s) 399, 1065
Bbv12I GWGCWC 1 cut(s) 1131
BccI CCATC 6 cut(s) 350, 584, 772, 904, 909, 1136
BciT130I CCWGG 4 cut(s) 163, 200, 1121, 1152
BclI TGATCA 2 cut(s) 493, 1102
BcnI CCSGG 1 cut(s) 153
BcoDI GTCTC 5 cut(s) 178, 221, 477, 725, 976
BfaI CTAG 4 cut(s) 372, 446, 794, 1253
Bme1390I CCNGG 5 cut(s) 153, 163, 200, 1121, 1152
Bme18I GGWCC 1 cut(s) 1166
BmeT110I CYCGRG 1 cut(s) 114
BmgT120I GGNCC 2 cut(s) 517, 1166
BmiI GGNNCC 4 cut(s) 925, 1000, 1148, 1167
BmrFI CCNGG 5 cut(s) 153, 163, 200, 1121, 1152
BmsI GCATC 1 cut(s) 601
BmtI GCTAGC 1 cut(s) 797
BpiI GAAGAC 2 cut(s) 399, 1065
BpuEI CTTGAG 4 cut(s) 10, 122, 232, 958
BpuMI CCSGG 1 cut(s) 153
Bsa29I ATCGAT 1 cut(s) 861
BsaI GGTCTC 1 cut(s) 178
BsaJI CCNNGG 4 cut(s) 61, 513, 1120, 1150
BsaXI ACNNNNNCTCC 2 cut(s) 86, 116
Bsc4I CCNNNNNNNGG 2 cut(s) 152, 205
Bse1I ACTGG 2 cut(s) 253, 591
Bse3DI GCAATG 1 cut(s) 630
BseBI CCWGG 4 cut(s) 163, 200, 1121, 1152
BseCI ATCGAT 1 cut(s) 861
BseDI CCNNGG 4 cut(s) 61, 513, 1120, 1150
BseGI GGATG 8 cut(s) 565, 595, 783, 896, 1027, 1077, 1147, 1222
BseLI CCNNNNNNNGG 2 cut(s) 152, 205
BseMI GCAATG 1 cut(s) 630
BseNI ACTGG 2 cut(s) 253, 591
BseRI GAGGAG 1 cut(s) 91
BseSI GKGCMC 2 cut(s) 1131, 1151
BseYI CCCAGC 1 cut(s) 529
BshFI GGCC 2 cut(s) 209, 519
BshNI GGYRCC 2 cut(s) 923, 1146
BshVI ATCGAT 1 cut(s) 861
BsiHKAI GWGCWC 1 cut(s) 1131
BsiHKCI CYCGRG 1 cut(s) 114
BsiSI CCGG 1 cut(s) 153
BslFI GGGAC 2 cut(s) 135, 1179
BslI CCNNNNNNNGG 2 cut(s) 152, 205
BsmAI GTCTC 5 cut(s) 178, 221, 477, 725, 976
BsmBI CGTCTC 1 cut(s) 976
BsmFI GGGAC 2 cut(s) 135, 1179
BsnI GGCC 2 cut(s) 209, 519
Bso31I GGTCTC 1 cut(s) 178
BsoBI CYCGRG 1 cut(s) 114
Bsp1286I GDGCHC 2 cut(s) 1131, 1151
Bsp1407I TGTACA 2 cut(s) 955, 1065
Bsp19I CCATGG 1 cut(s) 61
BspACI CCGC 3 cut(s) 130, 313, 320
BspANI GGCC 2 cut(s) 209, 519
BspDI ATCGAT 1 cut(s) 861
BspHI TCATGA 1 cut(s) 946
BspLI GGNNCC 4 cut(s) 925, 1000, 1148, 1167
BspOI GCTAGC 1 cut(s) 797
BspPI GGATC 3 cut(s) 367, 814, 866
BspT107I GGYRCC 2 cut(s) 923, 1146
BspTNI GGTCTC 1 cut(s) 178
BsrDI GCAATG 1 cut(s) 630
BsrGI TGTACA 2 cut(s) 955, 1065
BsrI ACTGG 2 cut(s) 253, 591
BssECI CCNNGG 4 cut(s) 61, 513, 1120, 1150
BssSI CACGAG 1 cut(s) 225
BssT1I CCWWGG 1 cut(s) 61
Bst2BI CACGAG 1 cut(s) 225
Bst2UI CCWGG 4 cut(s) 163, 200, 1121, 1152
Bst4CI ACNGT 4 cut(s) 241, 433, 908, 970
Bst6I CTCTTC 1 cut(s) 1172
BstAPI GCANNNNNTGC 1 cut(s) 625
BstAUI TGTACA 2 cut(s) 955, 1065
BstC8I GCNNGC 2 cut(s) 621, 795
BstDEI CTNAG 1 cut(s) 1229
BstDSI CCRYGG 2 cut(s) 61, 513
BstEII GGTNACC 1 cut(s) 724
BstF5I GGATG 8 cut(s) 565, 595, 783, 896, 1027, 1077, 1147, 1222
BstHHI GCGC 1 cut(s) 784
BstMAI GTCTC 5 cut(s) 178, 221, 477, 725, 976
BstMWI GCNNNNNNNGC 4 cut(s) 530, 620, 625, 790
BstNI CCWGG 4 cut(s) 163, 200, 1121, 1152
BstNSI RCATGY 2 cut(s) 581, 623
BstPI GGTNACC 1 cut(s) 724
BstSCI CCNGG 5 cut(s) 151, 161, 198, 1119, 1150
BstSLI GKGCMC 2 cut(s) 1131, 1151
BstV2I GAAGAC 2 cut(s) 399, 1065
BstX2I RGATCY 1 cut(s) 806
BstXI CCANNNNNNTGG 1 cut(s) 169
BstYI RGATCY 1 cut(s) 806
Bsu15I ATCGAT 1 cut(s) 861
BsuRI GGCC 2 cut(s) 209, 519
BsuTUI ATCGAT 1 cut(s) 861
BtgI CCRYGG 2 cut(s) 61, 513
BtsCI GGATG 8 cut(s) 565, 595, 783, 896, 1027, 1077, 1147, 1222
BtsI GCAGTG 2 cut(s) 275, 678
BtsIMutI CAGTG 3 cut(s) 246, 275, 678
Cac8I GCNNGC 2 cut(s) 621, 795
CciI TCATGA 1 cut(s) 946
CfoI GCGC 1 cut(s) 784
Cfr13I GGNCC 2 cut(s) 517, 1166
ClaI ATCGAT 1 cut(s) 861
Csp6I GTAC 4 cut(s) 429, 956, 1066, 1158
CspCI CAANNNNNGTGG 4 cut(s) 502, 537, 639, 674
CviQI GTAC 4 cut(s) 429, 956, 1066, 1158
DdeI CTNAG 1 cut(s) 1229
Eam1104I CTCTTC 1 cut(s) 1172
EarI CTCTTC 1 cut(s) 1172
Eco130I CCWWGG 1 cut(s) 61
Eco31I GGTCTC 1 cut(s) 178
Eco47I GGWCC 1 cut(s) 1166
Eco88I CYCGRG 1 cut(s) 114
Eco91I GGTNACC 1 cut(s) 724
EcoO109I RGGNCCY 1 cut(s) 1166
EcoO65I GGTNACC 1 cut(s) 724
EcoRII CCWGG 4 cut(s) 161, 198, 1119, 1150
EcoT14I CCWWGG 1 cut(s) 61
ErhI CCWWGG 1 cut(s) 61
Esp3I CGTCTC 1 cut(s) 976
FalI AAGNNNNNCTT 2 cut(s) 1067, 1099
FaqI GGGAC 2 cut(s) 135, 1179
FauNDI CATATG 1 cut(s) 688
FbaI TGATCA 2 cut(s) 493, 1102
FokI GGATG 8 cut(s) 572, 602, 790, 883, 1034, 1084, 1154, 1229
FspBI CTAG 4 cut(s) 372, 446, 794, 1253
GlaI GCGC 1 cut(s) 783
GsaI CCCAGC 1 cut(s) 533
HaeIII GGCC 2 cut(s) 209, 519
HapII CCGG 1 cut(s) 153
HhaI GCGC 1 cut(s) 784
Hin6I GCGC 1 cut(s) 782
HinP1I GCGC 1 cut(s) 782
HincII GTYRAC 1 cut(s) 750
HindII GTYRAC 1 cut(s) 750
HindIII AAGCTT 1 cut(s) 1190
HinfI GANTC 8 cut(s) 15, 119, 142, 203, 214, 283, 759, 962
HpaII CCGG 1 cut(s) 153
HphI GGTGA 6 cut(s) 63, 353, 452, 673, 736, 896
Hpy166II GTNNAC 3 cut(s) 750, 814, 1129
Hpy188I TCNGA 3 cut(s) 92, 294, 1102
Hpy8I GTNNAC 3 cut(s) 750, 814, 1129
Hpy99I CGWCG 1 cut(s) 110
HpyAV CCTTC 2 cut(s) 1069, 1200
HpyCH4III ACNGT 4 cut(s) 241, 433, 908, 970
HpyCH4V TGCA 3 cut(s) 623, 628, 1129
HpyF10VI GCNNNNNNNGC 4 cut(s) 530, 620, 625, 790
HpyF3I CTNAG 1 cut(s) 1229
HspAI GCGC 1 cut(s) 782
Ksp22I TGATCA 2 cut(s) 493, 1102
LmnI GCTCC 2 cut(s) 165, 538
LweI GCATC 1 cut(s) 601
MaeI CTAG 4 cut(s) 372, 446, 794, 1253
MaeIII GTNAC 2 cut(s) 598, 724
MboII GAAGA 4 cut(s) 404, 1058, 1070, 1189
MflI RGATCY 1 cut(s) 806
MhlI GDGCHC 2 cut(s) 1131, 1151
MluCI AATT 6 cut(s) 55, 83, 124, 737, 800, 1210
MlyI GAGTC 2 cut(s) 197, 223
MmeI TCCRAC 1 cut(s) 441
MseI TTAA 3 cut(s) 450, 471, 1185
MslI CAYNNNNRTG 6 cut(s) 401, 771, 906, 945, 951, 957
MspI CCGG 1 cut(s) 153
MspR9I CCNGG 5 cut(s) 153, 163, 200, 1121, 1152
MvaI CCWGG 4 cut(s) 163, 200, 1121, 1152
MwoI GCNNNNNNNGC 4 cut(s) 530, 620, 625, 790
NciI CCSGG 1 cut(s) 153
NcoI CCATGG 1 cut(s) 61
NdeI CATATG 1 cut(s) 688
NheI GCTAGC 1 cut(s) 793
NlaIV GGNNCC 4 cut(s) 925, 1000, 1148, 1167
NmeAIII GCCGAG 1 cut(s) 185
NmuCI GTSAC 1 cut(s) 724
NspI RCATGY 2 cut(s) 581, 623
PaeI GCATGC 1 cut(s) 623
PagI TCATGA 1 cut(s) 946
PciI ACATGT 1 cut(s) 577
PcsI WCGNNNNNNNCGW 1 cut(s) 858
PfeI GAWTC 6 cut(s) 15, 119, 142, 283, 759, 962
PflFI GACNNNGTC 1 cut(s) 398
PfoI TCCNGGA 2 cut(s) 151, 161
PleI GAGTC 2 cut(s) 197, 222
PpsI GAGTC 2 cut(s) 197, 222
PpuMI RGGWCCY 1 cut(s) 1166
PscI ACATGT 1 cut(s) 577
Psp5II RGGWCCY 1 cut(s) 1166
Psp6I CCWGG 4 cut(s) 161, 198, 1119, 1150
PspEI GGTNACC 1 cut(s) 724
PspFI CCCAGC 1 cut(s) 529
PspGI CCWGG 4 cut(s) 161, 198, 1119, 1150
PspN4I GGNNCC 4 cut(s) 925, 1000, 1148, 1167
PspPI GGNCC 2 cut(s) 517, 1166
PspPPI RGGWCCY 1 cut(s) 1166
PsuI RGATCY 1 cut(s) 806
PsyI GACNNNGTC 1 cut(s) 398
RsaI GTAC 4 cut(s) 430, 957, 1067, 1159
RsaNI GTAC 4 cut(s) 429, 956, 1066, 1158
RseI CAYNNNNRTG 6 cut(s) 401, 771, 906, 945, 951, 957
SaqAI TTAA 3 cut(s) 450, 471, 1185
Sau96I GGNCC 2 cut(s) 517, 1166
SchI GAGTC 2 cut(s) 197, 223
ScrFI CCNGG 5 cut(s) 153, 163, 200, 1121, 1152
SduI GDGCHC 2 cut(s) 1131, 1151
SfaNI GCATC 1 cut(s) 601
SinI GGWCC 1 cut(s) 1166
SmiMI CAYNNNNRTG 6 cut(s) 401, 771, 906, 945, 951, 957
SmlI CTYRAG 4 cut(s) 25, 137, 211, 973
SmoI CTYRAG 4 cut(s) 25, 137, 211, 973
SphI GCATGC 1 cut(s) 623
Sse9I AATT 6 cut(s) 55, 83, 124, 737, 800, 1210
SsiI CCGC 3 cut(s) 130, 313, 320
SspMI CTAG 4 cut(s) 372, 446, 794, 1253
StyD4I CCNGG 5 cut(s) 151, 161, 198, 1119, 1150
StyI CCWWGG 1 cut(s) 61
TaaI ACNGT 4 cut(s) 241, 433, 908, 970
TaqI TCGA 5 cut(s) 195, 219, 852, 861, 1044
TaqII GACCGA 1 cut(s) 743
TasI AATT 6 cut(s) 55, 83, 124, 737, 800, 1210
TatI WGTACW 4 cut(s) 428, 955, 1065, 1157
TfiI GAWTC 6 cut(s) 15, 119, 142, 283, 759, 962
Tru1I TTAA 3 cut(s) 450, 471, 1185
Tru9I TTAA 3 cut(s) 450, 471, 1185
TscAI CASTG 3 cut(s) 253, 282, 678
TseFI GTSAC 1 cut(s) 724
Tsp45I GTSAC 1 cut(s) 724
TspDTI ATGAA 7 cut(s) 7, 76, 369, 576, 1088, 1189, 1233
TspGWI ACGGA 1 cut(s) 94
TspRI CASTG 3 cut(s) 253, 282, 678
Tth111I GACNNNGTC 1 cut(s) 398
VneI GTGCAC 1 cut(s) 1127
VpaK11BI GGWCC 1 cut(s) 1166
XapI RAATTY 2 cut(s) 800, 1210
XceI RCATGY 2 cut(s) 581, 623
XcmI CCANNNNNNNNNTGG 1 cut(s) 1158
XspI CTAG 4 cut(s) 372, 446, 794, 1253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.