Prupe.3G092800_v2.0.a1

COBW domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0001734
Physical Location & Seq
Reverse (-)
6933929 .. 6939100
5172 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G092800.1

Sequence Viewer

Length: 1344 bp
ATGGCTTCGATATCGGCCGACCTGGCCACGACCCTTCTCGGCCTCACTTCTCGCCACTCAACCCGGCTCCCCCGGGTCGGAGCCGCGGCTCTGTTCCCTCTCACTTGGAGACCAAGAACTCCCCCTCACTCCCAAAGCCTACGCTACTCGACGTCGTCGTTTTGCCGAACACTCGTCGCCAAGCCTAAGCTCGGCTGCAGAAGGTTCACCGTGGCTGCGACCGCAACGACGTCGCAGAGCGAAAGCTCCGACGTTTTGACCCAGATTGTCCCCGATAATCGCATCCCCGCCACGATAATCACCGGCTTCTTGGGCTCCGGAAAGACGACATTGCTTAACCATATACTAACTGCGGAGCATGGGAAGCGAATTGCGGTGATTGAGAACGAGTATGGTGAAATAGACATTGATGGTTCTTTGGTTGCTGCAAAAACCACTGGGGCAGAAGATATTGTTATGTTGAATAATGGGTGTCTTTGCTGCACTGTGAGGGGTGACCTTGTTCGTATGATTTCGGACTTGGTCCATAAGAAGAAAGGGAAGTTCGATCATATTGTAATAGAGACTACAGGATTGGCAAATCCAGCACCGATCATTCAAACATTTTATGCTGAGGATCAGGTTTTTAATGATGTCAAGTTGGATGGTGTTGTCACGTTAGTTGATGCTAAACATGCTGTTTTTCATCTGAATGAAGTTAAGCCAAAAGGAGTTGTCAATGAGGCTATAGAGCAGATTGCTTATGCTGACCGTATCATCGTGAATAAGACCGATCTTGTTGGTGAGCCAGAAATTGCTTCTTTGGTGCAACGAATAAGGAACATCAATGGTATGGCTCAACTGAAGCGGACAGAGTTTGGAAAAGTTAACCTGGATTATGTTCTTGGTATTGGAGGATTTGATTTGGAGAGGATAGAGAGTGCTATTGACGCTGAAGGTGAAAAGGAAGATCATGATCATCACGATCATCATCACCATCATGACGAGAAACACGACCACAAGCACGAACATCATGATGATCACCACACCCATGACCATGTTCATGATCCTGGCGTTTCTTCTGTCAGCATAGTTTGTGAAGGGAACTTAGATCTTGAAAAGGCTAACATATGGCTCGGTACACTGTTGTTGGAACGGAGCGAGGACATATATCGTATGAAAGGTCTTCTATCTGTTCAGGGAATGAATGAGAGATTTGTCTTCCAGGGAGTTCACGATATATTCCAAGGCTCACCTGATCGGCTGTGGGGCCCCAACGAGCCAAGAACAAACAAGATTGTTTTCATAGGGAAGAATTTGGATGCACAAGAGTTAGAAAAGGGTTTTAAAGGCTGTTTACTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CobW_C cobW

Protein Analysis

448

Amino Acids

49.51

Weight (kDa)

6.07

Isoelectric Point (pI)

33.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 155, 233
AccII CGCG 1 cut(s) 86
AccIII TCCGGA 1 cut(s) 317
AciI CCGC 7 cut(s) 84, 86, 222, 288, 353, 374, 847
AclWI GGATC 2 cut(s) 624, 1040
AcoI YGGCCR 2 cut(s) 15, 24
AcsI RAATTY 1 cut(s) 1294
AcuI CTGAAG 2 cut(s) 863, 954
AcyI GRCGYC 2 cut(s) 152, 230
AfaI GTAC 1 cut(s) 1120
AfiI CCNNNNNNNGG 2 cut(s) 77, 191
AgsI TTSAA 3 cut(s) 463, 599, 1097
AjnI CCWGG 4 cut(s) 21, 870, 1048, 1203
AluBI AGCT 2 cut(s) 190, 246
AluI AGCT 2 cut(s) 190, 246
Alw26I GTCTC 2 cut(s) 103, 557
AlwI GGATC 2 cut(s) 624, 1040
Ama87I CYCGRG 1 cut(s) 72
Aor13HI TCCGGA 1 cut(s) 317
AoxI GGCC 4 cut(s) 15, 24, 40, 1249
ApaI GGGCCC 1 cut(s) 1253
ApeKI GCWGC 4 cut(s) 195, 215, 425, 480
ApoI RAATTY 1 cut(s) 1294
AspS9I GGNCC 3 cut(s) 523, 1249, 1250
AsuC2I CCSGG 3 cut(s) 64, 73, 74
AvaI CYCGRG 1 cut(s) 72
AvaII GGWCC 1 cut(s) 523
BaeGI GKGCMC 1 cut(s) 1253
BalI TGGCCA 1 cut(s) 26
BanII GRGCYC 2 cut(s) 317, 1253
BbsI GAAGAC 2 cut(s) 1157, 1192
BbvCI CCTCAGC 1 cut(s) 612
BbvI GCAGC 4 cut(s) 182, 202, 412, 467
BccI CCATC 3 cut(s) 404, 638, 984
BcgI CGANNNNNNTGC 2 cut(s) 213, 247
BciT130I CCWGG 4 cut(s) 23, 872, 1050, 1205
BclI TGATCA 2 cut(s) 955, 1018
BcnI CCSGG 3 cut(s) 64, 73, 74
BcoDI GTCTC 2 cut(s) 103, 557
BfmI CTRYAG 3 cut(s) 196, 567, 726
BglI GCCNNNNNGGC 1 cut(s) 23
BglII AGATCT 1 cut(s) 1090
BisI GCNGC 6 cut(s) 84, 87, 196, 216, 426, 481
BlsI GCNGC 6 cut(s) 85, 88, 197, 217, 427, 482
Bme1390I CCNGG 7 cut(s) 23, 64, 73, 74, 872, 1050, 1205
Bme18I GGWCC 1 cut(s) 523
BmeT110I CYCGRG 1 cut(s) 72
BmgT120I GGNCC 3 cut(s) 523, 1249, 1250
BmiI GGNNCC 6 cut(s) 68, 82, 316, 1250, 1251, 1252
BmrFI CCNGG 7 cut(s) 23, 64, 73, 74, 872, 1050, 1205
BmrI ACTGGG 1 cut(s) 447
BmsI GCATC 3 cut(s) 291, 655, 1291
BmuI ACTGGG 1 cut(s) 447
BpiI GAAGAC 2 cut(s) 1157, 1192
Bpu10I CCTNAGC 2 cut(s) 186, 612
BpuMI CCSGG 3 cut(s) 64, 73, 74
BsaBI GATNNNNATC 2 cut(s) 954, 969
BsaHI GRCGYC 2 cut(s) 152, 230
BsaI GGTCTC 1 cut(s) 103
BsaJI CCNNGG 6 cut(s) 71, 72, 84, 210, 1204, 1225
BsaWI WCCGGW 1 cut(s) 317
Bsc4I CCNNNNNNNGG 2 cut(s) 77, 191
Bse118I RCCGGY 1 cut(s) 302
Bse1I ACTGG 1 cut(s) 442
Bse3DI GCAATG 1 cut(s) 329
Bse8I GATNNNNATC 2 cut(s) 954, 969
BseAI TCCGGA 1 cut(s) 317
BseBI CCWGG 4 cut(s) 23, 872, 1050, 1205
BseDI CCNNGG 6 cut(s) 71, 72, 84, 210, 1204, 1225
BseGI GGATG 3 cut(s) 282, 649, 1306
BseJI GATNNNNATC 2 cut(s) 954, 969
BseLI CCNNNNNNNGG 2 cut(s) 77, 191
BseMI GCAATG 1 cut(s) 329
BseMII CTCAG 1 cut(s) 603
BseNI ACTGG 1 cut(s) 442
BseSI GKGCMC 1 cut(s) 1253
BseX3I CGGCCG 1 cut(s) 15
BseXI GCAGC 4 cut(s) 182, 202, 412, 467
BsgI GTGCAG 1 cut(s) 466
Bsh1236I CGCG 1 cut(s) 86
Bsh1285I CGRYCG 2 cut(s) 18, 222
BshFI GGCC 4 cut(s) 17, 26, 42, 1251
BsiEI CGRYCG 2 cut(s) 18, 222
BsiHKCI CYCGRG 1 cut(s) 72
BsiSI CCGG 4 cut(s) 64, 73, 303, 318
BslFI GGGAC 1 cut(s) 254
BslI CCNNNNNNNGG 2 cut(s) 77, 191
BsmAI GTCTC 2 cut(s) 103, 557
BsmFI GGGAC 1 cut(s) 254
BsnI GGCC 4 cut(s) 17, 26, 42, 1251
Bso31I GGTCTC 1 cut(s) 103
BsoBI CYCGRG 1 cut(s) 72
Bsp120I GGGCCC 1 cut(s) 1249
Bsp1286I GDGCHC 2 cut(s) 317, 1253
Bsp13I TCCGGA 1 cut(s) 317
BspACI CCGC 7 cut(s) 84, 86, 222, 288, 353, 374, 847
BspANI GGCC 4 cut(s) 17, 26, 42, 1251
BspCNI CTCAG 1 cut(s) 604
BspEI TCCGGA 1 cut(s) 317
BspFNI CGCG 1 cut(s) 86
BspHI TCATGA 4 cut(s) 952, 979, 1012, 1042
BspLI GGNNCC 6 cut(s) 68, 82, 316, 1250, 1251, 1252
BspMAI CTGCAG 1 cut(s) 200
BspPI GGATC 2 cut(s) 624, 1040
BspTNI GGTCTC 1 cut(s) 103
BsrDI GCAATG 1 cut(s) 329
BsrFI RCCGGY 1 cut(s) 302
BsrI ACTGG 1 cut(s) 442
BssAI RCCGGY 1 cut(s) 302
BssECI CCNNGG 6 cut(s) 71, 72, 84, 210, 1204, 1225
BssNI GRCGYC 2 cut(s) 152, 230
BssT1I CCWWGG 1 cut(s) 1225
Bst2UI CCWGG 4 cut(s) 23, 872, 1050, 1205
Bst4CI ACNGT 4 cut(s) 211, 487, 752, 1125
BstACI GRCGYC 2 cut(s) 152, 230
BstDEI CTNAG 3 cut(s) 186, 612, 1087
BstDSI CCRYGG 2 cut(s) 84, 210
BstEII GGTNACC 1 cut(s) 494
BstF5I GGATG 3 cut(s) 282, 649, 1306
BstFNI CGCG 1 cut(s) 86
BstMAI GTCTC 2 cut(s) 103, 557
BstMCI CGRYCG 2 cut(s) 18, 222
BstMWI GCNNNNNNNGC 7 cut(s) 23, 221, 312, 364, 584, 674, 929
BstNI CCWGG 4 cut(s) 23, 872, 1050, 1205
BstNSI RCATGY 1 cut(s) 677
BstPI GGTNACC 1 cut(s) 494
BstSCI CCNGG 7 cut(s) 21, 62, 71, 72, 870, 1048, 1203
BstSFI CTRYAG 3 cut(s) 196, 567, 726
BstSLI GKGCMC 1 cut(s) 1253
BstUI CGCG 1 cut(s) 86
BstV1I GCAGC 4 cut(s) 182, 202, 412, 467
BstV2I GAAGAC 2 cut(s) 1157, 1192
BstX2I RGATCY 1 cut(s) 1090
BstYI RGATCY 1 cut(s) 1090
BstZI CGGCCG 1 cut(s) 15
BsuRI GGCC 4 cut(s) 17, 26, 42, 1251
BtgI CCRYGG 2 cut(s) 84, 210
BtsCI GGATG 3 cut(s) 282, 649, 1306
BtsIMutI CAGTG 3 cut(s) 435, 483, 1121
CciI TCATGA 4 cut(s) 952, 979, 1012, 1042
Cfr10I RCCGGY 1 cut(s) 302
Cfr13I GGNCC 3 cut(s) 523, 1249, 1250
Cfr42I CCGCGG 1 cut(s) 87
Cfr9I CCCGGG 1 cut(s) 72
CseI GACGC 1 cut(s) 938
Csp6I GTAC 1 cut(s) 1119
CviAII CATG 8 cut(s) 359, 674, 953, 980, 1013, 1031, 1037, 1043
CviQI GTAC 1 cut(s) 1119
DdeI CTNAG 3 cut(s) 186, 612, 1087
DraI TTTAAA 1 cut(s) 1327
EaeI YGGCCR 2 cut(s) 15, 24
EagI CGGCCG 1 cut(s) 15
EclXI CGGCCG 1 cut(s) 15
Eco130I CCWWGG 1 cut(s) 1225
Eco24I GRGCYC 2 cut(s) 317, 1253
Eco31I GGTCTC 1 cut(s) 103
Eco32I GATATC 1 cut(s) 12
Eco47I GGWCC 1 cut(s) 523
Eco52I CGGCCG 1 cut(s) 15
Eco57I CTGAAG 2 cut(s) 863, 954
Eco88I CYCGRG 1 cut(s) 72
Eco91I GGTNACC 1 cut(s) 494
EcoO109I RGGNCCY 2 cut(s) 1249, 1250
EcoO65I GGTNACC 1 cut(s) 494
EcoRII CCWGG 4 cut(s) 21, 870, 1048, 1203
EcoRV GATATC 1 cut(s) 12
EcoT14I CCWWGG 1 cut(s) 1225
EcoT38I GRGCYC 2 cut(s) 317, 1253
ErhI CCWWGG 1 cut(s) 1225
FaeI CATG 8 cut(s) 362, 677, 956, 983, 1016, 1034, 1040, 1046
FaqI GGGAC 1 cut(s) 254
FatI CATG 8 cut(s) 358, 673, 952, 979, 1012, 1030, 1036, 1042
FauI CCCGC 1 cut(s) 295
FauNDI CATATG 1 cut(s) 1109
FbaI TGATCA 2 cut(s) 955, 1018
Fnu4HI GCNGC 6 cut(s) 84, 87, 196, 216, 426, 481
FokI GGATG 3 cut(s) 269, 656, 1313
FriOI GRGCYC 2 cut(s) 317, 1253
Fsp4HI GCNGC 6 cut(s) 84, 87, 196, 216, 426, 481
GluI GCNGC 6 cut(s) 84, 87, 196, 216, 426, 481
HaeIII GGCC 4 cut(s) 17, 26, 42, 1251
HapII CCGG 4 cut(s) 64, 73, 303, 318
HgaI GACGC 1 cut(s) 938
Hin1I GRCGYC 2 cut(s) 152, 230
Hin1II CATG 8 cut(s) 362, 677, 956, 983, 1016, 1034, 1040, 1046
HincII GTYRAC 1 cut(s) 868
HindII GTYRAC 1 cut(s) 868
HpaI GTTAAC 1 cut(s) 868
HpaII CCGG 4 cut(s) 64, 73, 303, 318
Hpy166II GTNNAC 5 cut(s) 207, 868, 1121, 1213, 1337
Hpy188I TCNGA 4 cut(s) 80, 250, 517, 690
Hpy188III TCNNGA 9 cut(s) 318, 760, 953, 962, 980, 1013, 1043, 1094, 1214
Hpy8I GTNNAC 5 cut(s) 207, 868, 1121, 1213, 1337
Hpy99I CGWCG 7 cut(s) 154, 157, 160, 179, 232, 235, 254
HpyAV CCTTC 4 cut(s) 44, 195, 929, 1073
HpyCH4III ACNGT 4 cut(s) 211, 487, 752, 1125
HpyCH4IV ACGT 4 cut(s) 152, 230, 252, 656
HpyCH4V TGCA 5 cut(s) 198, 428, 483, 808, 1304
HpyF10VI GCNNNNNNNGC 7 cut(s) 23, 221, 312, 364, 584, 674, 929
HpyF3I CTNAG 3 cut(s) 186, 612, 1087
HpySE526I ACGT 4 cut(s) 152, 230, 252, 656
Hsp92I GRCGYC 2 cut(s) 152, 230
Hsp92II CATG 8 cut(s) 362, 677, 956, 983, 1016, 1034, 1040, 1046
Kpn2I TCCGGA 1 cut(s) 317
Ksp22I TGATCA 2 cut(s) 955, 1018
KspAI GTTAAC 1 cut(s) 868
KspI CCGCGG 1 cut(s) 87
LmnI GCTCC 6 cut(s) 72, 80, 251, 320, 355, 1137
Lsp1109I GCAGC 4 cut(s) 182, 202, 412, 467
LweI GCATC 3 cut(s) 291, 655, 1291
MaeII ACGT 4 cut(s) 152, 230, 252, 656
MaeIII GTNAC 2 cut(s) 494, 652
MboII GAAGA 7 cut(s) 458, 544, 959, 1050, 1157, 1192, 1303
MflI RGATCY 1 cut(s) 1090
MhlI GDGCHC 2 cut(s) 317, 1253
MlsI TGGCCA 1 cut(s) 26
MluCI AATT 3 cut(s) 369, 792, 1294
MluNI TGGCCA 1 cut(s) 26
MmeI TCCRAC 4 cut(s) 58, 273, 621, 1110
MnlI CCTC 9 cut(s) 53, 108, 135, 483, 607, 715, 887, 903, 1135
Mox20I TGGCCA 1 cut(s) 26
MroI TCCGGA 1 cut(s) 317
MscI TGGCCA 1 cut(s) 26
MseI TTAA 5 cut(s) 336, 627, 699, 867, 1326
MslI CAYNNNNRTG 6 cut(s) 690, 978, 1014, 1029, 1035, 1041
Msp20I TGGCCA 1 cut(s) 26
MspA1I CMGCKG 1 cut(s) 86
MspI CCGG 4 cut(s) 64, 73, 303, 318
MspR9I CCNGG 7 cut(s) 23, 64, 73, 74, 872, 1050, 1205
MvaI CCWGG 4 cut(s) 23, 872, 1050, 1205
MvnI CGCG 1 cut(s) 86
MwoI GCNNNNNNNGC 7 cut(s) 23, 221, 312, 364, 584, 674, 929
NciI CCSGG 3 cut(s) 64, 73, 74
NdeI CATATG 1 cut(s) 1109
NlaIII CATG 8 cut(s) 362, 677, 956, 983, 1016, 1034, 1040, 1046
NlaIV GGNNCC 6 cut(s) 68, 82, 316, 1250, 1251, 1252
NmeAIII GCCGAG 2 cut(s) 18, 171
NmuCI GTSAC 2 cut(s) 494, 652
NspI RCATGY 1 cut(s) 677
PagI TCATGA 4 cut(s) 952, 979, 1012, 1042
PcsI WCGNNNNNNNCGW 2 cut(s) 155, 990
PflFI GACNNNGTC 2 cut(s) 154, 521
PkrI GCNGC 6 cut(s) 85, 88, 197, 217, 427, 482
Psp6I CCWGG 4 cut(s) 21, 870, 1048, 1203
PspEI GGTNACC 1 cut(s) 494
PspGI CCWGG 4 cut(s) 21, 870, 1048, 1203
PspN4I GGNNCC 6 cut(s) 68, 82, 316, 1250, 1251, 1252
PspOMI GGGCCC 1 cut(s) 1249
PspPI GGNCC 3 cut(s) 523, 1249, 1250
PstI CTGCAG 1 cut(s) 200
PsuI RGATCY 1 cut(s) 1090
PsyI GACNNNGTC 2 cut(s) 154, 521
RsaI GTAC 1 cut(s) 1120
RsaNI GTAC 1 cut(s) 1119
RseI CAYNNNNRTG 6 cut(s) 690, 978, 1014, 1029, 1035, 1041
SacII CCGCGG 1 cut(s) 87
SaqAI TTAA 5 cut(s) 336, 627, 699, 867, 1326
SatI GCNGC 6 cut(s) 84, 87, 196, 216, 426, 481
Sau96I GGNCC 3 cut(s) 523, 1249, 1250
ScrFI CCNGG 7 cut(s) 23, 64, 73, 74, 872, 1050, 1205
SduI GDGCHC 2 cut(s) 317, 1253
SfaNI GCATC 3 cut(s) 291, 655, 1291
SfcI CTRYAG 3 cut(s) 196, 567, 726
SfiI GGCCNNNNNGGCC 1 cut(s) 23
Sfr303I CCGCGG 1 cut(s) 87
SgrBI CCGCGG 1 cut(s) 87
SinI GGWCC 1 cut(s) 523
SmaI CCCGGG 1 cut(s) 74
SmiMI CAYNNNNRTG 6 cut(s) 690, 978, 1014, 1029, 1035, 1041
Sse9I AATT 3 cut(s) 369, 792, 1294
SsiI CCGC 7 cut(s) 84, 86, 222, 288, 353, 374, 847
StyD4I CCNGG 7 cut(s) 21, 62, 71, 72, 870, 1048, 1203
StyI CCWWGG 1 cut(s) 1225
TaaI ACNGT 4 cut(s) 211, 487, 752, 1125
TaiI ACGT 4 cut(s) 155, 233, 255, 659
TaqI TCGA 3 cut(s) 8, 149, 546
TaqII GACCGA 1 cut(s) 785
TasI AATT 3 cut(s) 369, 792, 1294
TauI GCSGC 2 cut(s) 86, 89
Tru1I TTAA 5 cut(s) 336, 627, 699, 867, 1326
Tru9I TTAA 5 cut(s) 336, 627, 699, 867, 1326
TscAI CASTG 3 cut(s) 442, 490, 1128
TseFI GTSAC 2 cut(s) 494, 652
TseI GCWGC 4 cut(s) 195, 215, 425, 480
Tsp45I GTSAC 2 cut(s) 494, 652
TspDTI ATGAA 6 cut(s) 674, 708, 1031, 1172, 1199, 1273
TspGWI ACGGA 1 cut(s) 1150
TspMI CCCGGG 1 cut(s) 72
TspRI CASTG 3 cut(s) 442, 490, 1128
Tth111I GACNNNGTC 2 cut(s) 154, 521
VpaK11BI GGWCC 1 cut(s) 523
XapI RAATTY 1 cut(s) 1294
XceI RCATGY 1 cut(s) 677
XmaI CCCGGG 1 cut(s) 72
ZraI GACGTC 2 cut(s) 153, 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.