Rh7DG277500

COBW domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0001734
Physical Location & Seq
Forward (+)
29901318 .. 29905945
4628 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG277500.1

Sequence Viewer

Length: 1086 bp
ATGGTCTCTGCCTCAAGCTACAATCTTGACGAGAATCCGAGCTTGGGTCTCGATACCCGTGTTCCGGCGACCATTATCACCGGCTTTCTCGGTTCCGGAAAGACTACATTGCTCAATCATATACTAACATCTCAACATGGAAAGCGGATTGCGGTCATAGAAAATGAGTTTGGTGAGGTGGATATTGATGGGTCATTGGTTGCTAGTCATTCATCATCAAATGAAGACATTGTCATGGTTAATAATGGTTGCCTATGCTGTACTGTTCGGGGTGATCTAGTAAAAATGCTCTTGCAATTGGTTAAGGAAAAGCGAGACAAGTTTGATCATATTGTCATAGAAACCACAGGCCTCGCTAAGCCATCACCTGTTATAGAAACGTTTTGTATGGATGAGCTAGTTTCACGACATGTGAAACTGGATGGAGTTGTTACTTTGGTGGATTGCAAGCATGCAATGCAGCATCTGAATGAAGTTAAACCAAGATTTGTGGTGAATGAGGCAGTAGAACAAGTTGCATATGCTGATCGTATTATATTGAACAAGATAGATTTGGTAACCGAGGTGGAGTTAGAGACATTGACCAAGAAAATCAAGCATATCAATGGGATGGCGCAAATCAAGCTAGCTAAATTTGGATCCGTGGACATGGACTTTGTTCTGGGTGTGGGAGGATATGATCTCGAAAGGATTGATAATGAAGTTCAAGTCGATAGTTCCTGTACTTCCTCACATCACCATGAAGCTGGACATGAACATGACAACCATCACCACGACCATGTACATGATTCTACTGTCTCAAGTGTCAGTATTGTTGCCGAGGGAACCCTTGATCTTGATGAGGTGGATGACTGGTTGGAAAGACTTATTGAAGAAAAGGGGGAGGACTTGTACAGGATGAAGGGCGTTTTATGTGTTAACGGTTCTGATCAACGTTATGTTTTCCAGGGAGTGCACTCTACGTTGGATGGGTGCCCAGGGGGCACATGGGGACCCGATGAGAAGAGGATTAACAAGCTTGTGTTCATAGGAAGGAATCTGGATGAAACTAGTCTTAGGAAAGGTTTCAGAGGTTGTCTAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CobW_C cobW

Protein Analysis

361

Amino Acids

39.93

Weight (kDa)

5.28

Isoelectric Point (pI)

32.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cobW PF02492 22 - 207 3e-59 CobW/HypB/UreG, nucleotide-binding domain
CobW_C PF07683 266 - 360 4.8e-24 Cobalamin synthesis protein cobW C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 972
AccIII TCCGGA 1 cut(s) 95
AciI CCGC 2 cut(s) 145, 152
AclI AACGTT 2 cut(s) 380, 934
AclWI GGATC 2 cut(s) 633, 646
AcsI RAATTY 1 cut(s) 632
AfaI GTAC 4 cut(s) 262, 724, 783, 893
AfiI CCNNNNNNNGG 2 cut(s) 44, 64
AflIII ACRYGT 1 cut(s) 409
AgsI TTSAA 3 cut(s) 541, 707, 872
AhlI ACTAGT 1 cut(s) 1049
AjnI CCWGG 2 cut(s) 945, 976
AjuI GAANNNNNNNTTGG 4 cut(s) 26, 58, 153, 185
AluBI AGCT 7 cut(s) 18, 42, 397, 625, 629, 746, 1018
AluI AGCT 7 cut(s) 18, 42, 397, 625, 629, 746, 1018
Alw21I GWGCWC 1 cut(s) 957
Alw26I GTCTC 5 cut(s) 10, 53, 309, 569, 802
Alw44I GTGCAC 1 cut(s) 953
AlwI GGATC 2 cut(s) 633, 646
AlwNI CAGNNNCTG 1 cut(s) 466
Aor13HI TCCGGA 1 cut(s) 95
AoxI GGCC 1 cut(s) 349
ApaLI GTGCAC 1 cut(s) 953
ApeKI GCWGC 1 cut(s) 460
ApoI RAATTY 1 cut(s) 632
ArsI GACNNNNNNTTYG 2 cut(s) 638, 670
Asp700I GAANNNNTTC 1 cut(s) 1064
AspLEI GCGC 1 cut(s) 616
AspS9I GGNCC 1 cut(s) 992
AsuHPI GGTGA 7 cut(s) 70, 185, 284, 357, 505, 728, 761
AsuNHI GCTAGC 1 cut(s) 625
AvaII GGWCC 1 cut(s) 992
BaeGI GKGCMC 3 cut(s) 957, 977, 986
BaeI ACNNNNGTAYC 2 cut(s) 45, 78
BamHI GGATCC 1 cut(s) 638
BanI GGYRCC 1 cut(s) 972
BbsI GAAGAC 1 cut(s) 231
Bbv12I GWGCWC 1 cut(s) 957
BbvI GCAGC 1 cut(s) 472
BccI CCATC 6 cut(s) 182, 370, 416, 604, 774, 962
BciT130I CCWGG 2 cut(s) 947, 978
BclI TGATCA 2 cut(s) 325, 928
BcoDI GTCTC 5 cut(s) 10, 53, 309, 569, 802
BcuI ACTAGT 1 cut(s) 1049
BfaI CTAG 6 cut(s) 204, 278, 398, 626, 1050, 1079
BglI GCCNNNNNGGC 1 cut(s) 981
BisI GCNGC 1 cut(s) 461
BlpI GCTNAGC 1 cut(s) 357
BlsI GCNGC 1 cut(s) 462
Bme1390I CCNGG 2 cut(s) 947, 978
Bme18I GGWCC 1 cut(s) 992
BmgT120I GGNCC 1 cut(s) 992
BmiI GGNNCC 6 cut(s) 94, 640, 826, 974, 993, 994
BmrFI CCNGG 2 cut(s) 947, 978
BmsI GCATC 1 cut(s) 472
BmtI GCTAGC 1 cut(s) 629
BpiI GAAGAC 1 cut(s) 231
Bpu1102I GCTNAGC 1 cut(s) 357
BpuEI CTTGAG 1 cut(s) 784
BsaI GGTCTC 2 cut(s) 10, 53
BsaJI CCNNGG 6 cut(s) 561, 642, 819, 946, 976, 977
BsaWI WCCGGW 1 cut(s) 95
BsaXI ACNNNNNCTCC 2 cut(s) 875, 905
Bsc4I CCNNNNNNNGG 2 cut(s) 44, 64
Bse118I RCCGGY 1 cut(s) 80
Bse1I ACTGG 2 cut(s) 423, 857
Bse3DI GCAATG 2 cut(s) 107, 462
BseAI TCCGGA 1 cut(s) 95
BseBI CCWGG 2 cut(s) 947, 978
BseDI CCNNGG 6 cut(s) 561, 642, 819, 946, 976, 977
BseGI GGATG 7 cut(s) 397, 427, 615, 853, 903, 973, 1048
BseLI CCNNNNNNNGG 2 cut(s) 44, 64
BseMI GCAATG 2 cut(s) 107, 462
BseNI ACTGG 2 cut(s) 423, 857
BseSI GKGCMC 3 cut(s) 957, 977, 986
BseXI GCAGC 1 cut(s) 472
BshFI GGCC 1 cut(s) 351
BshNI GGYRCC 1 cut(s) 972
BsiHKAI GWGCWC 1 cut(s) 957
BsiSI CCGG 3 cut(s) 65, 81, 96
BslFI GGGAC 1 cut(s) 1005
BslI CCNNNNNNNGG 2 cut(s) 44, 64
BsmAI GTCTC 5 cut(s) 10, 53, 309, 569, 802
BsmFI GGGAC 1 cut(s) 1005
BsnI GGCC 1 cut(s) 351
Bso31I GGTCTC 2 cut(s) 10, 53
Bsp1286I GDGCHC 3 cut(s) 957, 977, 986
Bsp13I TCCGGA 1 cut(s) 95
Bsp1407I TGTACA 2 cut(s) 781, 891
Bsp143I GATC 7 cut(s) 274, 325, 526, 638, 679, 832, 928
Bsp1720I GCTNAGC 1 cut(s) 357
BspACI CCGC 2 cut(s) 145, 152
BspANI GGCC 1 cut(s) 351
BspEI TCCGGA 1 cut(s) 95
BspLI GGNNCC 6 cut(s) 94, 640, 826, 974, 993, 994
BspOI GCTAGC 1 cut(s) 629
BspPI GGATC 2 cut(s) 633, 646
BspT107I GGYRCC 1 cut(s) 972
BspTNI GGTCTC 2 cut(s) 10, 53
BsrDI GCAATG 2 cut(s) 107, 462
BsrFI RCCGGY 1 cut(s) 80
BsrGI TGTACA 2 cut(s) 781, 891
BsrI ACTGG 2 cut(s) 423, 857
BssAI RCCGGY 1 cut(s) 80
BssECI CCNNGG 6 cut(s) 561, 642, 819, 946, 976, 977
BssMI GATC 7 cut(s) 274, 325, 526, 638, 679, 832, 928
Bst2UI CCWGG 2 cut(s) 947, 978
Bst4CI ACNGT 3 cut(s) 265, 796, 923
Bst6I CTCTTC 1 cut(s) 998
BstAPI GCANNNNNTGC 1 cut(s) 457
BstAUI TGTACA 2 cut(s) 781, 891
BstC8I GCNNGC 3 cut(s) 449, 453, 627
BstDEI CTNAG 2 cut(s) 357, 1055
BstDSI CCRYGG 1 cut(s) 642
BstEII GGTNACC 1 cut(s) 556
BstF5I GGATG 7 cut(s) 397, 427, 615, 853, 903, 973, 1048
BstHHI GCGC 1 cut(s) 616
BstKTI GATC 7 cut(s) 277, 328, 529, 641, 682, 835, 931
BstMAI GTCTC 5 cut(s) 10, 53, 309, 569, 802
BstMBI GATC 7 cut(s) 274, 325, 526, 638, 679, 832, 928
BstMWI GCNNNNNNNGC 3 cut(s) 457, 622, 981
BstNI CCWGG 2 cut(s) 947, 978
BstNSI RCATGY 2 cut(s) 413, 455
BstPI GGTNACC 1 cut(s) 556
BstSCI CCNGG 2 cut(s) 945, 976
BstSLI GKGCMC 3 cut(s) 957, 977, 986
BstV1I GCAGC 1 cut(s) 472
BstV2I GAAGAC 1 cut(s) 231
BstX2I RGATCY 1 cut(s) 638
BstXI CCANNNNNNTGG 1 cut(s) 746
BstYI RGATCY 1 cut(s) 638
BsuRI GGCC 1 cut(s) 351
BtgI CCRYGG 1 cut(s) 642
BtsCI GGATG 7 cut(s) 397, 427, 615, 853, 903, 973, 1048
Cac8I GCNNGC 3 cut(s) 449, 453, 627
CaiI CAGNNNCTG 1 cut(s) 466
CfoI GCGC 1 cut(s) 616
Cfr10I RCCGGY 1 cut(s) 80
Cfr13I GGNCC 1 cut(s) 992
Csp6I GTAC 4 cut(s) 261, 723, 782, 892
CspCI CAANNNNNGTGG 2 cut(s) 471, 506
CviQI GTAC 4 cut(s) 261, 723, 782, 892
DdeI CTNAG 2 cut(s) 357, 1055
DpnI GATC 7 cut(s) 276, 327, 528, 640, 681, 834, 930
DpnII GATC 7 cut(s) 274, 325, 526, 638, 679, 832, 928
Eam1104I CTCTTC 1 cut(s) 998
EarI CTCTTC 1 cut(s) 998
Eco147I AGGCCT 1 cut(s) 351
Eco31I GGTCTC 2 cut(s) 10, 53
Eco47I GGWCC 1 cut(s) 992
Eco91I GGTNACC 1 cut(s) 556
EcoO109I RGGNCCY 1 cut(s) 992
EcoO65I GGTNACC 1 cut(s) 556
EcoRII CCWGG 2 cut(s) 945, 976
FaqI GGGAC 1 cut(s) 1005
FauNDI CATATG 1 cut(s) 520
FbaI TGATCA 2 cut(s) 325, 928
Fnu4HI GCNGC 1 cut(s) 461
FokI GGATG 7 cut(s) 404, 434, 622, 860, 910, 980, 1055
Fsp4HI GCNGC 1 cut(s) 461
FspBI CTAG 6 cut(s) 204, 278, 398, 626, 1050, 1079
GlaI GCGC 1 cut(s) 615
GluI GCNGC 1 cut(s) 461
HaeIII GGCC 1 cut(s) 351
HapII CCGG 3 cut(s) 65, 81, 96
HhaI GCGC 1 cut(s) 616
Hin6I GCGC 1 cut(s) 614
HinP1I GCGC 1 cut(s) 614
HincII GTYRAC 1 cut(s) 919
HindII GTYRAC 1 cut(s) 919
HindIII AAGCTT 1 cut(s) 1016
HinfI GANTC 3 cut(s) 34, 788, 1036
HpaI GTTAAC 1 cut(s) 919
HpaII CCGG 3 cut(s) 65, 81, 96
HphI GGTGA 7 cut(s) 70, 185, 284, 357, 505, 728, 761
Hpy166II GTNNAC 3 cut(s) 646, 919, 955
Hpy188I TCNGA 4 cut(s) 39, 468, 928, 1070
Hpy188III TCNNGA 7 cut(s) 26, 50, 96, 405, 683, 836, 1040
Hpy8I GTNNAC 3 cut(s) 646, 919, 955
HpyAV CCTTC 2 cut(s) 895, 1026
HpyCH4III ACNGT 3 cut(s) 265, 796, 923
HpyCH4IV ACGT 3 cut(s) 380, 934, 962
HpyCH4V TGCA 6 cut(s) 295, 447, 455, 460, 518, 955
HpyF10VI GCNNNNNNNGC 3 cut(s) 457, 622, 981
HpyF3I CTNAG 2 cut(s) 357, 1055
HpySE526I ACGT 3 cut(s) 380, 934, 962
HspAI GCGC 1 cut(s) 614
KflI GGGWCCC 1 cut(s) 992
Kpn2I TCCGGA 1 cut(s) 95
Ksp22I TGATCA 2 cut(s) 325, 928
KspAI GTTAAC 1 cut(s) 919
Kzo9I GATC 7 cut(s) 274, 325, 526, 638, 679, 832, 928
Lsp1109I GCAGC 1 cut(s) 472
LweI GCATC 1 cut(s) 472
MaeI CTAG 6 cut(s) 204, 278, 398, 626, 1050, 1079
MaeII ACGT 3 cut(s) 380, 934, 962
MaeIII GTNAC 2 cut(s) 430, 556
MalI GATC 7 cut(s) 276, 327, 528, 640, 681, 834, 930
MboI GATC 7 cut(s) 274, 325, 526, 638, 679, 832, 928
MboII GAAGA 3 cut(s) 236, 884, 1015
MfeI CAATTG 1 cut(s) 296
MflI RGATCY 1 cut(s) 638
MhlI GDGCHC 3 cut(s) 957, 977, 986
MluCI AATT 2 cut(s) 296, 632
MmeI TCCRAC 2 cut(s) 837, 945
MroI TCCGGA 1 cut(s) 95
MroXI GAANNNNTTC 1 cut(s) 1064
MseI TTAA 5 cut(s) 240, 303, 477, 918, 1011
MslI CAYNNNNRTG 7 cut(s) 233, 468, 603, 738, 756, 777, 783
MspI CCGG 3 cut(s) 65, 81, 96
MspR9I CCNGG 2 cut(s) 947, 978
MunI CAATTG 1 cut(s) 296
MvaI CCWGG 2 cut(s) 947, 978
MwoI GCNNNNNNNGC 3 cut(s) 457, 622, 981
NdeI CATATG 1 cut(s) 520
NdeII GATC 7 cut(s) 274, 325, 526, 638, 679, 832, 928
NheI GCTAGC 1 cut(s) 625
NlaIV GGNNCC 6 cut(s) 94, 640, 826, 974, 993, 994
NmeAIII GCCGAG 1 cut(s) 844
NspI RCATGY 2 cut(s) 413, 455
PaeI GCATGC 1 cut(s) 455
PasI CCCWGGG 1 cut(s) 977
PceI AGGCCT 1 cut(s) 351
PciI ACATGT 1 cut(s) 409
PdmI GAANNNNTTC 1 cut(s) 1064
PfeI GAWTC 3 cut(s) 34, 788, 1036
PflFI GACNNNGTC 1 cut(s) 230
PkrI GCNGC 1 cut(s) 462
PpuMI RGGWCCY 1 cut(s) 992
PscI ACATGT 1 cut(s) 409
Psp1406I AACGTT 2 cut(s) 380, 934
Psp5II RGGWCCY 1 cut(s) 992
Psp6I CCWGG 2 cut(s) 945, 976
PspEI GGTNACC 1 cut(s) 556
PspGI CCWGG 2 cut(s) 945, 976
PspN4I GGNNCC 6 cut(s) 94, 640, 826, 974, 993, 994
PspPI GGNCC 1 cut(s) 992
PspPPI RGGWCCY 1 cut(s) 992
PstNI CAGNNNCTG 1 cut(s) 466
PsuI RGATCY 1 cut(s) 638
PsyI GACNNNGTC 1 cut(s) 230
RsaI GTAC 4 cut(s) 262, 724, 783, 893
RsaNI GTAC 4 cut(s) 261, 723, 782, 892
RseI CAYNNNNRTG 7 cut(s) 233, 468, 603, 738, 756, 777, 783
SaqAI TTAA 5 cut(s) 240, 303, 477, 918, 1011
SatI GCNGC 1 cut(s) 461
Sau3AI GATC 7 cut(s) 274, 325, 526, 638, 679, 832, 928
Sau96I GGNCC 1 cut(s) 992
ScrFI CCNGG 2 cut(s) 947, 978
SduI GDGCHC 3 cut(s) 957, 977, 986
SfaNI GCATC 1 cut(s) 472
SinI GGWCC 1 cut(s) 992
SmiMI CAYNNNNRTG 7 cut(s) 233, 468, 603, 738, 756, 777, 783
SmlI CTYRAG 2 cut(s) 13, 799
SmoI CTYRAG 2 cut(s) 13, 799
SpeI ACTAGT 1 cut(s) 1049
SphI GCATGC 1 cut(s) 455
Sse9I AATT 2 cut(s) 296, 632
SseBI AGGCCT 1 cut(s) 351
SsiI CCGC 2 cut(s) 145, 152
SspMI CTAG 6 cut(s) 204, 278, 398, 626, 1050, 1079
StuI AGGCCT 1 cut(s) 351
StyD4I CCNGG 2 cut(s) 945, 976
TaaI ACNGT 3 cut(s) 265, 796, 923
TaiI ACGT 3 cut(s) 383, 937, 965
TaqI TCGA 3 cut(s) 51, 684, 711
TasI AATT 2 cut(s) 296, 632
TatI WGTACW 4 cut(s) 260, 722, 781, 891
TfiI GAWTC 3 cut(s) 34, 788, 1036
Tru1I TTAA 5 cut(s) 240, 303, 477, 918, 1011
Tru9I TTAA 5 cut(s) 240, 303, 477, 918, 1011
TseI GCWGC 1 cut(s) 460
TspDTI ATGAA 9 cut(s) 201, 237, 486, 714, 756, 768, 914, 1015, 1059
TspGWI ACGGA 1 cut(s) 631
Tth111I GACNNNGTC 1 cut(s) 230
VneI GTGCAC 1 cut(s) 953
VpaK11BI GGWCC 1 cut(s) 992
XapI RAATTY 1 cut(s) 632
XceI RCATGY 2 cut(s) 413, 455
XcmI CCANNNNNNNNNTGG 1 cut(s) 984
XmnI GAANNNNTTC 1 cut(s) 1064
XspI CTAG 6 cut(s) 204, 278, 398, 626, 1050, 1079
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.