MD17G1216800.v1.1

COBW domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0001734
Physical Location & Seq
Reverse (-)
26368628 .. 26374583
5956 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1216800.v1.1.491

Sequence Viewer

Length: 1362 bp
ATGGCTTCCATATCGGCGGACATCGCCACGACCCTTACCGGGCTAACTTCTCCCCGCTCCACCCGATTCCCCAGGGTCGGAGCCGCAGCCCTCTTTCCTTTCACGTGGAGACCGAGGACTTTCGCACGCTCCCAAACCCTACACTACTCGGTGTCGTCGTTTTGCCGACCTCTTGTCGCCAAGCCCAACCGAGGCTACAGAAGGTTCACTGCGGCTGCGAGCAGCGCAACGACGTCGCAGAGCGAGAGCTCCGACGTTTTGACCCAGATTGAGCCGGATAATCGCATCCCCGCCACCATAATCACCGGTTTCTTGGGCTCCGGAAAGACTACATTGCTTAACCATATATTGACTGCGGAACACGGGAAGCGAATTGCGGTGATTGAGAACGAGTATGGTGAGATAGACATTGACGGTTCTTTGGTTGCTGCGAAAACCACTGAGGCCGAAGACATTGTTATGTTGAATAATGGTTGCCTTTGCTGCACTGTGAGGGGTGATCTTGTGCGGATGATTGCGGACTTGGTCGATAAGAAGAAAGGGAAGTTTGATCATATTGTTATAGAGACTACGGGATTGGCAAATCCGGCACCGATCATTCAAACCTTTTATGCTGAAGATCAGGTTTTTAATGATGTCAAGTTGGACGGTGTTGTAACATTAGTTGATGCTAAGCATGCTGTTTTTCATTTGGATGAAGTTAAGCCAAAAGGAGTTGTCAATGAGGCTATAGAGCAGATTGCTTATGCTGACCGTATCATTGTCAATAAGACCGATCTTGTTGGTGAGCCAGAAATTGCTTCTTTGGTGCAACGAATTAAGAACATCAATGGCATGGCTCAATTGAAGCGGACTGAGTTTGGGAAAGTTAACCTGGATTATGTTCTTGGTATTGGAGGATTTGATTTGGAGAGGATAGAGAGTGCTATTGATGCTGAAGGTGAAAAGGAAGATCATGACCATCACCATGACCATGACCATGACCATCATCACCATCATGATGAGAAACACAATCACAAGCATGATCATCATGATGATCACCACGCCCATGATCATGTTCACGATCCTGGTGTTTCTTCTGTGAGCATAGTTTGTGAAGGGAACTTAGATCTTGAAAAGGCTAACATATGGCTTGGTACGCTGTTGTTGGAACGAAGCGACGACATATATCGTATGAAAGGTCTTCTATCTGTTCAAGGAATGAATGAGAGATTTGTCTTCCAGGGAGTTCACGATATATTCCAAGGCTCACCTGATCGGCCGTGGGGGCCCAACGAGCCAAGAACGAACAAGATTGTTTTCATAGGGAAGAACTTGGATGCGCAGGAGTTGGAAAAGGGTTTTAAAGGCTGTTTACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CobW_C cobW

Protein Analysis

454

Amino Acids

50.3

Weight (kDa)

5.83

Isoelectric Point (pI)

35.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cobW PF02492 97 - 281 5.2e-59 CobW/HypB/UreG, nucleotide-binding domain
CobW_C PF07683 358 - 452 1e-22 Cobalamin synthesis protein cobW C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 236
Acc16I TGCGCA 1 cut(s) 1323
AccB1I GGYRCC 1 cut(s) 589
AccBSI CCGCTC 1 cut(s) 57
AccIII TCCGGA 1 cut(s) 320
AclWI GGATC 1 cut(s) 1058
AcoI YGGCCR 1 cut(s) 1259
AcuI CTGAAG 2 cut(s) 636, 957
AcvI CACGTG 1 cut(s) 105
AcyI GRCGYC 1 cut(s) 233
AfaI GTAC 1 cut(s) 1138
AfiI CCNNNNNNNGG 3 cut(s) 39, 77, 191
AgeI ACCGGT 1 cut(s) 305
AgsI TTSAA 5 cut(s) 466, 602, 847, 1115, 1196
AhdI GACNNNNNGTC 1 cut(s) 173
AjnI CCWGG 4 cut(s) 71, 873, 1066, 1221
AluBI AGCT 1 cut(s) 249
AluI AGCT 1 cut(s) 249
Alw21I GWGCWC 1 cut(s) 251
Alw26I GTCTC 2 cut(s) 103, 560
AlwI GGATC 1 cut(s) 1058
Aor13HI TCCGGA 1 cut(s) 320
AoxI GGCC 3 cut(s) 444, 1259, 1268
ApaI GGGCCC 1 cut(s) 1272
ApeKI GCWGC 5 cut(s) 86, 215, 222, 428, 483
AsiGI ACCGGT 1 cut(s) 305
AspLEI GCGC 2 cut(s) 227, 1324
AspS9I GGNCC 2 cut(s) 1268, 1269
AsuC2I CCSGG 1 cut(s) 40
BaeGI GKGCMC 1 cut(s) 1272
BanI GGYRCC 1 cut(s) 589
BanII GRGCYC 3 cut(s) 251, 320, 1272
BbrPI CACGTG 1 cut(s) 105
BbsI GAAGAC 3 cut(s) 456, 1175, 1210
Bbv12I GWGCWC 1 cut(s) 251
BbvI GCAGC 5 cut(s) 98, 202, 234, 415, 470
BccI CCATC 3 cut(s) 969, 993, 1002
BceAI ACGGC 1 cut(s) 1246
BcgI CGANNNNNNTGC 2 cut(s) 216, 250
BciT130I CCWGG 4 cut(s) 73, 875, 1068, 1223
BclI TGATCA 4 cut(s) 550, 1024, 1036, 1051
BcnI CCSGG 1 cut(s) 40
BcoDI GTCTC 2 cut(s) 103, 560
BfmI CTRYAG 2 cut(s) 196, 729
BglI GCCNNNNNGGC 1 cut(s) 1267
BglII AGATCT 1 cut(s) 1108
BisI GCNGC 7 cut(s) 84, 87, 213, 216, 223, 429, 484
BlpI GCTNAGC 1 cut(s) 672
BlsI GCNGC 7 cut(s) 85, 88, 214, 217, 224, 430, 485
Bme1390I CCNGG 5 cut(s) 40, 73, 875, 1068, 1223
BmeRI GACNNNNNGTC 1 cut(s) 173
BmgT120I GGNCC 2 cut(s) 1268, 1269
BmiI GGNNCC 5 cut(s) 82, 319, 591, 1269, 1270
BmrFI CCNGG 5 cut(s) 40, 73, 875, 1068, 1223
BmsI GCATC 4 cut(s) 294, 658, 922, 1309
BpiI GAAGAC 3 cut(s) 456, 1175, 1210
Bpu1102I GCTNAGC 1 cut(s) 672
BpuMI CCSGG 1 cut(s) 40
BsaAI YACGTR 1 cut(s) 105
BsaHI GRCGYC 1 cut(s) 233
BsaI GGTCTC 1 cut(s) 103
BsaJI CCNNGG 7 cut(s) 71, 72, 113, 190, 1222, 1243, 1262
BsaWI WCCGGW 2 cut(s) 305, 320
Bsc4I CCNNNNNNNGG 3 cut(s) 39, 77, 191
Bse118I RCCGGY 1 cut(s) 305
Bse3DI GCAATG 1 cut(s) 332
BseAI TCCGGA 1 cut(s) 320
BseBI CCWGG 4 cut(s) 73, 875, 1068, 1223
BseDI CCNNGG 7 cut(s) 71, 72, 113, 190, 1222, 1243, 1262
BseGI GGATG 4 cut(s) 285, 516, 700, 1324
BseLI CCNNNNNNNGG 3 cut(s) 39, 77, 191
BseMI GCAATG 1 cut(s) 332
BseMII CTCAG 2 cut(s) 432, 846
BseSI GKGCMC 1 cut(s) 1272
BseX3I CGGCCG 1 cut(s) 1259
BseXI GCAGC 5 cut(s) 98, 202, 234, 415, 470
BsgI GTGCAG 1 cut(s) 469
Bsh1285I CGRYCG 1 cut(s) 1262
BshFI GGCC 3 cut(s) 446, 1261, 1270
BshNI GGYRCC 1 cut(s) 589
BshTI ACCGGT 1 cut(s) 305
BsiEI CGRYCG 1 cut(s) 1262
BsiHKAI GWGCWC 1 cut(s) 251
BsiSI CCGG 5 cut(s) 39, 275, 306, 321, 587
BslI CCNNNNNNNGG 3 cut(s) 39, 77, 191
BsmAI GTCTC 2 cut(s) 103, 560
BsnI GGCC 3 cut(s) 446, 1261, 1270
Bso31I GGTCTC 1 cut(s) 103
Bsp120I GGGCCC 1 cut(s) 1268
Bsp1286I GDGCHC 3 cut(s) 251, 320, 1272
Bsp13I TCCGGA 1 cut(s) 320
Bsp1720I GCTNAGC 1 cut(s) 672
BspANI GGCC 3 cut(s) 446, 1261, 1270
BspCNI CTCAG 2 cut(s) 433, 847
BspEI TCCGGA 1 cut(s) 320
BspHI TCATGA 3 cut(s) 955, 997, 1030
BspLI GGNNCC 5 cut(s) 82, 319, 591, 1269, 1270
BspPI GGATC 1 cut(s) 1058
BspT107I GGYRCC 1 cut(s) 589
BspTNI GGTCTC 1 cut(s) 103
BsrBI CCGCTC 1 cut(s) 57
BsrDI GCAATG 1 cut(s) 332
BsrFI RCCGGY 1 cut(s) 305
BssAI RCCGGY 1 cut(s) 305
BssECI CCNNGG 7 cut(s) 71, 72, 113, 190, 1222, 1243, 1262
BssNI GRCGYC 1 cut(s) 233
BssT1I CCWWGG 1 cut(s) 1243
Bst2UI CCWGG 4 cut(s) 73, 875, 1068, 1223
Bst4CI ACNGT 5 cut(s) 416, 490, 650, 755, 1359
BstACI GRCGYC 1 cut(s) 233
BstBAI YACGTR 1 cut(s) 105
BstC8I GCNNGC 3 cut(s) 127, 220, 678
BstDEI CTNAG 4 cut(s) 441, 672, 855, 1105
BstDSI CCRYGG 1 cut(s) 1262
BstF5I GGATG 4 cut(s) 285, 516, 700, 1324
BstHHI GCGC 2 cut(s) 227, 1324
BstMAI GTCTC 2 cut(s) 103, 560
BstMCI CGRYCG 1 cut(s) 1262
BstMWI GCNNNNNNNGC 9 cut(s) 23, 224, 483, 587, 677, 932, 1138, 1267, 1276
BstNI CCWGG 4 cut(s) 73, 875, 1068, 1223
BstNSI RCATGY 1 cut(s) 680
BstSCI CCNGG 5 cut(s) 38, 71, 873, 1066, 1221
BstSFI CTRYAG 2 cut(s) 196, 729
BstSLI GKGCMC 1 cut(s) 1272
BstV1I GCAGC 5 cut(s) 98, 202, 234, 415, 470
BstV2I GAAGAC 3 cut(s) 456, 1175, 1210
BstX2I RGATCY 1 cut(s) 1108
BstYI RGATCY 1 cut(s) 1108
BstZI CGGCCG 1 cut(s) 1259
BsuRI GGCC 3 cut(s) 446, 1261, 1270
BtgI CCRYGG 1 cut(s) 1262
BtgZI GCGATG 1 cut(s) 7
BtsCI GGATG 4 cut(s) 285, 516, 700, 1324
BtsI GCAGTG 1 cut(s) 207
BtsIMutI CAGTG 3 cut(s) 207, 438, 486
Cac8I GCNNGC 3 cut(s) 127, 220, 678
CciI TCATGA 3 cut(s) 955, 997, 1030
CfoI GCGC 2 cut(s) 227, 1324
Cfr10I RCCGGY 1 cut(s) 305
Cfr13I GGNCC 2 cut(s) 1268, 1269
Csp6I GTAC 1 cut(s) 1137
CspAI ACCGGT 1 cut(s) 305
CviQI GTAC 1 cut(s) 1137
DdeI CTNAG 4 cut(s) 441, 672, 855, 1105
DraI TTTAAA 1 cut(s) 1345
DriI GACNNNNNGTC 1 cut(s) 173
EaeI YGGCCR 1 cut(s) 1259
EagI CGGCCG 1 cut(s) 1259
Eam1105I GACNNNNNGTC 1 cut(s) 173
EciI GGCGGA 1 cut(s) 32
Ecl136II GAGCTC 1 cut(s) 249
EclXI CGGCCG 1 cut(s) 1259
Eco130I CCWWGG 1 cut(s) 1243
Eco24I GRGCYC 3 cut(s) 251, 320, 1272
Eco31I GGTCTC 1 cut(s) 103
Eco52I CGGCCG 1 cut(s) 1259
Eco53kI GAGCTC 1 cut(s) 249
Eco57I CTGAAG 2 cut(s) 636, 957
Eco72I CACGTG 1 cut(s) 105
EcoICRI GAGCTC 1 cut(s) 249
EcoO109I RGGNCCY 1 cut(s) 1268
EcoRII CCWGG 4 cut(s) 71, 873, 1066, 1221
EcoT14I CCWWGG 1 cut(s) 1243
EcoT38I GRGCYC 3 cut(s) 251, 320, 1272
ErhI CCWWGG 1 cut(s) 1243
FauI CCCGC 2 cut(s) 62, 298
FauNDI CATATG 1 cut(s) 1127
FbaI TGATCA 4 cut(s) 550, 1024, 1036, 1051
Fnu4HI GCNGC 7 cut(s) 84, 87, 213, 216, 223, 429, 484
FokI GGATG 4 cut(s) 272, 523, 707, 1331
FriOI GRGCYC 3 cut(s) 251, 320, 1272
Fsp4HI GCNGC 7 cut(s) 84, 87, 213, 216, 223, 429, 484
FspI TGCGCA 1 cut(s) 1323
GlaI GCGC 2 cut(s) 226, 1323
GluI GCNGC 7 cut(s) 84, 87, 213, 216, 223, 429, 484
HaeIII GGCC 3 cut(s) 446, 1261, 1270
HapII CCGG 5 cut(s) 39, 275, 306, 321, 587
HhaI GCGC 2 cut(s) 227, 1324
Hin1I GRCGYC 1 cut(s) 233
Hin6I GCGC 2 cut(s) 225, 1322
HinP1I GCGC 2 cut(s) 225, 1322
HincII GTYRAC 1 cut(s) 871
HindII GTYRAC 1 cut(s) 871
HinfI GANTC 1 cut(s) 66
HpaI GTTAAC 1 cut(s) 871
HpaII CCGG 5 cut(s) 39, 275, 306, 321, 587
Hpy166II GTNNAC 5 cut(s) 207, 871, 1060, 1231, 1355
Hpy188I TCNGA 2 cut(s) 80, 253
Hpy188III TCNNGA 7 cut(s) 321, 956, 998, 1031, 1061, 1112, 1232
Hpy8I GTNNAC 5 cut(s) 207, 871, 1060, 1231, 1355
Hpy99I CGWCG 5 cut(s) 160, 235, 238, 257, 1163
HpyAV CCTTC 3 cut(s) 195, 932, 1091
HpyCH4III ACNGT 5 cut(s) 416, 490, 650, 755, 1359
HpyCH4IV ACGT 3 cut(s) 104, 233, 255
HpyCH4V TGCA 2 cut(s) 486, 811
HpyF10VI GCNNNNNNNGC 9 cut(s) 23, 224, 483, 587, 677, 932, 1138, 1267, 1276
HpyF3I CTNAG 4 cut(s) 441, 672, 855, 1105
HpySE526I ACGT 3 cut(s) 104, 233, 255
Hsp92I GRCGYC 1 cut(s) 233
HspAI GCGC 2 cut(s) 225, 1322
Kpn2I TCCGGA 1 cut(s) 320
Ksp22I TGATCA 4 cut(s) 550, 1024, 1036, 1051
KspAI GTTAAC 1 cut(s) 871
LmnI GCTCC 5 cut(s) 62, 80, 134, 254, 323
Lsp1109I GCAGC 5 cut(s) 98, 202, 234, 415, 470
LweI GCATC 4 cut(s) 294, 658, 922, 1309
MaeII ACGT 3 cut(s) 104, 233, 255
MaeIII GTNAC 1 cut(s) 655
MbiI CCGCTC 1 cut(s) 57
MboII GAAGA 8 cut(s) 461, 547, 629, 962, 1068, 1175, 1210, 1321
MfeI CAATTG 1 cut(s) 842
MflI RGATCY 1 cut(s) 1108
MhlI GDGCHC 3 cut(s) 251, 320, 1272
MluCI AATT 4 cut(s) 372, 795, 816, 842
MmeI TCCRAC 5 cut(s) 58, 276, 624, 1128, 1311
MnlI CCTC 9 cut(s) 101, 108, 180, 185, 436, 486, 718, 890, 906
MroI TCCGGA 1 cut(s) 320
MseI TTAA 6 cut(s) 339, 630, 702, 819, 870, 1344
MspI CCGG 5 cut(s) 39, 275, 306, 321, 587
MspR9I CCNGG 5 cut(s) 40, 73, 875, 1068, 1223
MunI CAATTG 1 cut(s) 842
MvaI CCWGG 4 cut(s) 73, 875, 1068, 1223
MwoI GCNNNNNNNGC 9 cut(s) 23, 224, 483, 587, 677, 932, 1138, 1267, 1276
NciI CCSGG 1 cut(s) 40
NdeI CATATG 1 cut(s) 1127
NlaIV GGNNCC 5 cut(s) 82, 319, 591, 1269, 1270
NsbI TGCGCA 1 cut(s) 1323
NspI RCATGY 1 cut(s) 680
PaeI GCATGC 1 cut(s) 680
PagI TCATGA 3 cut(s) 955, 997, 1030
PasI CCCWGGG 1 cut(s) 72
PcsI WCGNNNNNNNCGW 2 cut(s) 110, 155
PfeI GAWTC 1 cut(s) 66
PflFI GACNNNGTC 1 cut(s) 524
PinAI ACCGGT 1 cut(s) 305
PkrI GCNGC 7 cut(s) 85, 88, 214, 217, 224, 430, 485
PmaCI CACGTG 1 cut(s) 105
PmlI CACGTG 1 cut(s) 105
Ppu21I YACGTR 1 cut(s) 105
Psp124BI GAGCTC 1 cut(s) 251
Psp6I CCWGG 4 cut(s) 71, 873, 1066, 1221
PspCI CACGTG 1 cut(s) 105
PspGI CCWGG 4 cut(s) 71, 873, 1066, 1221
PspN4I GGNNCC 5 cut(s) 82, 319, 591, 1269, 1270
PspOMI GGGCCC 1 cut(s) 1268
PspPI GGNCC 2 cut(s) 1268, 1269
PsuI RGATCY 1 cut(s) 1108
PsyI GACNNNGTC 1 cut(s) 524
RsaI GTAC 1 cut(s) 1138
RsaNI GTAC 1 cut(s) 1137
SacI GAGCTC 1 cut(s) 251
SaqAI TTAA 6 cut(s) 339, 630, 702, 819, 870, 1344
SatI GCNGC 7 cut(s) 84, 87, 213, 216, 223, 429, 484
Sau96I GGNCC 2 cut(s) 1268, 1269
ScrFI CCNGG 5 cut(s) 40, 73, 875, 1068, 1223
SduI GDGCHC 3 cut(s) 251, 320, 1272
SfaNI GCATC 4 cut(s) 294, 658, 922, 1309
SfcI CTRYAG 2 cut(s) 196, 729
SfiI GGCCNNNNNGGCC 1 cut(s) 1267
SphI GCATGC 1 cut(s) 680
Sse9I AATT 4 cut(s) 372, 795, 816, 842
SstI GAGCTC 1 cut(s) 251
StyD4I CCNGG 5 cut(s) 38, 71, 873, 1066, 1221
StyI CCWWGG 1 cut(s) 1243
TaaI ACNGT 5 cut(s) 416, 490, 650, 755, 1359
TaiI ACGT 3 cut(s) 107, 236, 258
TaqI TCGA 1 cut(s) 528
TaqII GACCGA 2 cut(s) 127, 788
TasI AATT 4 cut(s) 372, 795, 816, 842
TauI GCSGC 2 cut(s) 86, 215
TfiI GAWTC 1 cut(s) 66
Tru1I TTAA 6 cut(s) 339, 630, 702, 819, 870, 1344
Tru9I TTAA 6 cut(s) 339, 630, 702, 819, 870, 1344
TscAI CASTG 3 cut(s) 214, 445, 493
TseI GCWGC 5 cut(s) 86, 215, 222, 428, 483
TspDTI ATGAA 5 cut(s) 677, 711, 1190, 1217, 1291
TspRI CASTG 3 cut(s) 214, 445, 493
Tth111I GACNNNGTC 1 cut(s) 524
XceI RCATGY 1 cut(s) 680
ZraI GACGTC 1 cut(s) 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.