AT1G21160

Contains the following InterPro domains Small GTP-binding protein (InterPro IPR005225), Translation elongation factor EFTu EF1A, domain 2 (InterPro IPR004161), Translation initiation factor 2 related (InterPro IPR015760), Protein synthesis factor, GTP-binding (InterPro IPR000795)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0002582
Physical Location & Seq
Reverse (-)
7407931 .. 7413295
5365 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G21160.1

Sequence Viewer

Length: 3279 bp
ATGGATCCGAGGAATTCGAGTGCGTGGGGTGGGGGTGATGGTCTCCCCCTTATCTCATCCATGGGAGGTGTTGAATCAAAGAATAGATGCTGTGTGACTGACGACGATGGCTCTATTGGTACGAAGCTATGTGAGAAACCTAAAGCTGAGGAGATTGATTTTTCTGCTCGGAAAAAGAAGTTGAAATCGAAGAAAGGCGGGTCTGTTAGCTTTGCTCTTCTCGATGTTGAAGATGACAAGGAGGAAGCAGAGGATGGAGACGACAAAAATCCTTCTATTAGGAGTGATGAGGTGGTTGGAGAAACTTCAATGAGTAAGAAAAAGAAGAAGGATAAGAGTGGCAAGCATGGGGTATGTGAAGAAGATGATGATGCGGACAAGATTCTTGCTCAACATGGGATAACAACTACTGTTTCAACAGGGCCTGAAGCGGAGATAGCTCTAGCTCAACCTGAGCAAGTTGCATCTGCAGATGGGGCTGTTGACAAGGACGGAGAAGAAAAGACTGTTGAGTCAGCTACAGCGAAGAAAAAGAAAAAGAAGAAGGACAAGGACAAGAAAGCAAGTGCTTCTTTAGCTATTTCTTCTGTGGAGGCTAAGGAGGATAGACAAGGGAAGAAAGATGTAAAGATTAAAGTAGCGGAAAAGAAAGTTCCTAAACATGTTCGAGAGAAGCAAGAGACTCTTGCGCGGTGGAAAGAAGCTGAAGATGGGAAGAAGAAGGAAGAAGAAGAGAGATTGAGGAAAGAGGAAGAGGAGCGCCGTATAGAGGAAGAGCGAGAAAGGGAAGCTGAAGAGATTAGGCAGAAGAGAAAGATCAGGAAAATGGAGAAGAAGCAAGAAGGCCTGATTCTAACAGCAAAGCAAAAGCGTGATGCTGCTAAGAATGAGGCTTTCAGAAAAAGGGTGTTGACTGATGCTGGAAGTCTTCTTGTTGCAGATAAAAACGGTGATTCCTCAAAACGTCCCATATATGGCAATAAAAACAAATTAGCTTGCAAAAAAGCAAACGATCCTGCTTCTGTCCAGGCTAAAGGTGATGGGGAAACCAAGGAAAATCATGCAGCAGATGAACCATGTACTTTACCTGACTTGGTTTCAGTTGAAGATAGAAGGGTTGGTATTATCGAATCAGTAGACACAGAAGAGACACATGAATCTGTTGATGTATCACAAGAGAATGGTGATGAGGAAGATGTGTGGGATGCAAAAACTAATTTTACCATCAAGGGTGATTCTGATGATGAGGAGGAAAAACCTCAGCCTGTGTTCAAGAAAGGTTTAAAAGACACTGCATCAAAGGCACATGATTCAGTTCCTGGCGCAGATAGACCAACAGTGAAGCCTGGAGGTTCTGGCAAGCCAAAAACAGCTGCCAAAAAAGCTATGCCTAAGGTGGATGATACTACCCGGCCGAAAGATACTTCTAAAAAGGATGAAGGCTTAGTACTGAATGAACTTGCTAAAGAAGTAGAAGAAAATCTTCGCTCTCCAATTTGCTGCATCATGGGTCATGTTGATTCTGGTAAAACAAAGCTGTTGGATTGCATCAGAGGAACAAATGTTCAGGAAGGTGAAGCTGGAGGTATTACTCAACAGATTGGTGCAACCTTTTTCCCTGCAGAAAACATCCGTGAGAGGACCAAAGAGTTACAAGCTAATGCTAAACTCAAGGTGCCAGGTATATTGGTTATCGATACACCTGGACACGAGTCATTCACAAATCTGCGGTCAAGGGGTTCAAATTTGTGTGACCTTGCAATTCTAGTGGTTGATATAATGCGAGGTCTAGAGCCACAAACCATAGAATCTCTTAATCTTTTGAGAAGAAGGAATGTAAAGTTCATTATTGCCTTGAATAAGGTGGATAGGCTATATGGGTGGGAAAAAAGCAAGAATGCTCCTATAAGGAAGACAATGATGCAACAAACGGGAGATGTGGTTAAGGAATTTAAAATGAGGCTAAACCGAGTTCAAAACCAGTTCCAAGAACAAGGACTCAACAGTATGCTTTATTACAAGAACAGAGAAATGGGAGAAACTATCAGTATCCTACCTGCTAGCGCTATTAGTGGGGAAGGGATTCCAGATCTTTTACTGTTCTTGGTTCAATGGGCTCAGAAAACGATGGTGGAGAAACTTACATATGTTGACAAAGTGCAGTGTACCGTCCTTGAGGTCAAAGTTATTGAAGGCCATGGTATTACAGTTGATGTTGTTTTGGTCAACGGTGTACTCCGTGAAGGTGATCAAATTGTTGTTTGTGGGTCACAGGGACCAATTGTAACAACTATTAGATCATTGTTGACTCCTTATCCTATGAACGAGATGCGGGTGACGGGTACCTATATGCCTCACAGAGAAGTAAAGGCTGCGCAGGGTATCAAAATTGCTGCACAGGGCCTTGAACACGCCATTGCTGGTACTGCCTTGCATGTGATTGGACCTAATGAGGACATGGAAGAAGCCAAGAAAAATGCCATGGAAGATATTGAGTCAGTTATGAACCGCATTGACAAAAGTGGTGAAGGCGTTTATGTACAAGCATCTACATTAGGGTCCTTGGAAGCATTGCTCGAGTTCTTGAAGTCCTCAGATGTAAAAATACCTGTCAGTGGTATTGGCATAGGACCTGTGCATAAGAAGGATATCATGAAGGCCGGGGTAATGCTAGAGAAGAAAAAAGAGTTTGCAACGATTTTGGCCTTTGATGTGAAAATAAGTGCAGAAGCTCGTGAACTTGCAGACAAAATGGGAGTAAAAATCTTCTGCGATGATACCATCTATTGTCTGTTCGATAAGTTTAAGAGTTACATAGAGGGTATCAAAGAGGAGAAAAAGAAAGAAACAGCTTGTGAAGCAGTTTTCCCATGTATCCTTCAGATTTTACCAAACCATATCTACAATCAGAGGGACCCAATTATCCTTGGAGTTAAAGTCAATGATGGAATACTAAAGGTTGGAACTCCCATTTGCATTGTTAAAAGGATTGAGAATGTAAGAGTGTTTATGGACATTGGGCGCGTCTCATCAATTAAGAATAACAACAACATGCCAGTTGATTATGCAAGGAAAGGACAGGAGGTGGCTATTAAGATCATTGCTTCTAACCCAGAAGAACAGAAAATGTTTGGGAGGCACTTTGGTGTAGACGACAGGCTTATCAGTCACATTTCTAGTCGATCCGTGGACGTAATCAGAACTAATTACTGGAATGAGTTATCTAACGACGAGAAGGATCTTGTTTTGAGACTTAAAAGGATATTCAAGATACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 ko03013 map00195 map01100 map03013
Pfam Domains
Protein Families
GTP_EFTU GTP_EFTU_D2 IF-2

Protein Analysis

1092

Amino Acids

121.12

Weight (kDa)

6.99

Isoelectric Point (pI)

37.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 498 - 707 6.2e-36 Elongation factor Tu GTP binding domain
MMR_HSR1 PF01926 501 - 621 3.7e-06 50S ribosome-binding GTPase
GTP_EFTU_D2 PF03144 735 - 809 2.5e-06 Elongation factor Tu domain 2
IF-2 PF11987 821 - 936 2e-22 Translation-initiation factor 2
GTP_EFTU_D4 PF14578 957 - 1052 2.8e-10 Elongation factor Tu domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 511
Acc16I TGCGCA 1 cut(s) 2376
Acc36I ACCTGC 1 cut(s) 2065
Acc65I GGTACC 1 cut(s) 2342
AccB1I GGYRCC 2 cut(s) 1675, 2342
AccI GTMKAC 2 cut(s) 1137, 3150
AccII CGCG 2 cut(s) 691, 3024
AciI CCGC 8 cut(s) 198, 374, 431, 641, 691, 1729, 2332, 2509
AclWI GGATC 4 cut(s) 12, 1007, 3177, 3246
AcoI YGGCCR 1 cut(s) 1412
AcsI RAATTY 3 cut(s) 13, 1744, 1949
AcuI CTGAAG 4 cut(s) 447, 726, 813, 2864
AfaI GTAC 8 cut(s) 121, 1081, 1449, 2167, 2235, 2344, 2425, 2541
AfeI AGCGCT 1 cut(s) 2065
AfiI CCNNNNNNNGG 3 cut(s) 769, 974, 2615
AflIII ACRYGT 1 cut(s) 661
AjnI CCWGG 5 cut(s) 1026, 1318, 1345, 1678, 1702
AleI CACNNNNGTG 1 cut(s) 3144
AloI GAACNNNNNNTCC 2 cut(s) 1548, 1580
Alw26I GTCTC 6 cut(s) 47, 252, 674, 1142, 3031, 3244
AlwI GGATC 4 cut(s) 12, 1007, 3177, 3246
AlwNI CAGNNNCTG 2 cut(s) 425, 1319
Ama87I CYCGRG 1 cut(s) 2576
Aor51HI AGCGCT 1 cut(s) 2065
AoxI GGCC 7 cut(s) 422, 844, 1412, 2194, 2401, 2658, 2703
ApeKI GCWGC 6 cut(s) 878, 1064, 1373, 1500, 2372, 2393
ApoI RAATTY 3 cut(s) 13, 1744, 1949
ArsI GACNNNNNNTTYG 2 cut(s) 1715, 1747
Asp700I GAANNNNTTC 2 cut(s) 1482, 2082
Asp718I GGTACC 1 cut(s) 2342
AspLEI GCGC 6 cut(s) 691, 762, 1325, 2066, 2377, 3024
AspS9I GGNCC 8 cut(s) 422, 1641, 2276, 2401, 2444, 2559, 2630, 2914
AsuC2I CCSGG 2 cut(s) 1411, 2662
AsuHPI GGTGA 9 cut(s) 47, 962, 1049, 1196, 1244, 1586, 2258, 2347, 2537
AsuNHI GCTAGC 1 cut(s) 2060
AvaI CYCGRG 1 cut(s) 2576
AvaII GGWCC 6 cut(s) 1641, 2276, 2444, 2559, 2630, 2914
AxyI CCTNAGG 1 cut(s) 1392
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 2 cut(s) 1675, 2342
BanII GRGCYC 1 cut(s) 2119
BarI GAAGNNNNNNTAC 2 cut(s) 1431, 1463
BauI CACGAG 2 cut(s) 1709, 2733
BbsI GAAGAC 2 cut(s) 920, 1919
BbvCI CCTCAGC 2 cut(s) 147, 1260
BbvI GCAGC 6 cut(s) 865, 1076, 1360, 1487, 2359, 2380
BceAI ACGGC 1 cut(s) 747
BciT130I CCWGG 5 cut(s) 1028, 1320, 1347, 1680, 1704
BciVI GTATCC 2 cut(s) 2060, 2885
BclI TGATCA 1 cut(s) 2248
BcnI CCSGG 2 cut(s) 1411, 2662
BcoDI GTCTC 6 cut(s) 47, 252, 674, 1142, 3031, 3244
BfaI CTAG 6 cut(s) 443, 1766, 1790, 2061, 2672, 3177
BfmI CTRYAG 3 cut(s) 468, 519, 1620
BfoI RGCGCY 2 cut(s) 763, 2067
BfuAI ACCTGC 1 cut(s) 2065
BfuI GTATCC 2 cut(s) 2060, 2885
BglII AGATCT 1 cut(s) 2089
BisI GCNGC 6 cut(s) 879, 1065, 1374, 1501, 2373, 2394
BlsI GCNGC 6 cut(s) 880, 1066, 1375, 1502, 2374, 2395
BmcAI AGTACT 1 cut(s) 1449
Bme1390I CCNGG 7 cut(s) 1028, 1320, 1347, 1411, 1680, 1704, 2662
Bme18I GGWCC 6 cut(s) 1641, 2276, 2444, 2559, 2630, 2914
BmeT110I CYCGRG 1 cut(s) 2576
BmgT120I GGNCC 8 cut(s) 422, 1641, 2276, 2401, 2444, 2559, 2630, 2914
BmiI GGNNCC 7 cut(s) 6, 1677, 2277, 2344, 2560, 2915, 2916
BmrFI CCNGG 7 cut(s) 1028, 1320, 1347, 1411, 1680, 1704, 2662
BmtI GCTAGC 1 cut(s) 2064
BoxI GACNNNNGTC 1 cut(s) 1711
BpiI GAAGAC 2 cut(s) 920, 1919
BpmI CTGGAG 2 cut(s) 1368, 1602
Bpu10I CCTNAGC 4 cut(s) 147, 453, 597, 1260
BpuEI CTTGAG 2 cut(s) 1655, 2195
BpuMI CCSGG 2 cut(s) 1411, 2662
Bsa29I ATCGAT 1 cut(s) 1695
BsaI GGTCTC 1 cut(s) 47
BsaJI CCNNGG 9 cut(s) 8, 60, 1050, 2197, 2481, 2562, 2661, 2926, 3186
BsaXI ACNNNNNCTCC 4 cut(s) 1926, 1956, 2922, 2952
Bsc4I CCNNNNNNNGG 3 cut(s) 769, 974, 2615
Bse1I ACTGG 3 cut(s) 1981, 3056, 3215
Bse21I CCTNAGG 1 cut(s) 1392
Bse3DI GCAATG 3 cut(s) 2415, 2570, 3099
BseBI CCWGG 5 cut(s) 1028, 1320, 1347, 1680, 1704
BseCI ATCGAT 1 cut(s) 1695
BseDI CCNNGG 9 cut(s) 8, 60, 1050, 2197, 2481, 2562, 2661, 2926, 3186
BseGI GGATG 6 cut(s) 56, 259, 1210, 1405, 1441, 1629
BseLI CCNNNNNNNGG 3 cut(s) 769, 974, 2615
BseMI GCAATG 3 cut(s) 2415, 2570, 3099
BseMII CTCAG 5 cut(s) 138, 444, 1274, 2132, 2607
BseNI ACTGG 3 cut(s) 1981, 3056, 3215
BseRI GAGGAG 4 cut(s) 164, 770, 1262, 2846
BseX3I CGGCCG 1 cut(s) 1412
BseXI GCAGC 6 cut(s) 865, 1076, 1360, 1487, 2359, 2380
BsgI GTGCAG 3 cut(s) 2180, 2379, 2745
Bsh1236I CGCG 2 cut(s) 691, 3024
Bsh1285I CGRYCG 1 cut(s) 1415
BshFI GGCC 7 cut(s) 424, 846, 1414, 2196, 2403, 2660, 2705
BshNI GGYRCC 2 cut(s) 1675, 2342
BshVI ATCGAT 1 cut(s) 1695
BsiEI CGRYCG 1 cut(s) 1415
BsiHKCI CYCGRG 1 cut(s) 2576
BsiSI CCGG 2 cut(s) 1411, 2661
BslFI GGGAC 3 cut(s) 951, 2289, 2927
BslI CCNNNNNNNGG 3 cut(s) 769, 974, 2615
BsmAI GTCTC 6 cut(s) 47, 252, 674, 1142, 3031, 3244
BsmBI CGTCTC 2 cut(s) 252, 3031
BsmFI GGGAC 3 cut(s) 951, 2289, 2927
BsmI GAATGC 1 cut(s) 1903
BsnI GGCC 7 cut(s) 424, 846, 1414, 2196, 2403, 2660, 2705
Bso31I GGTCTC 1 cut(s) 47
BsoBI CYCGRG 1 cut(s) 2576
Bsp1286I GDGCHC 1 cut(s) 2119
Bsp1407I TGTACA 1 cut(s) 2539
Bsp143I GATC 9 cut(s) 4, 816, 1012, 2089, 2248, 2297, 3096, 3182, 3238
Bsp19I CCATGG 3 cut(s) 60, 2197, 2481
BspACI CCGC 8 cut(s) 198, 374, 431, 641, 691, 1729, 2332, 2509
BspANI GGCC 7 cut(s) 424, 846, 1414, 2196, 2403, 2660, 2705
BspCNI CTCAG 5 cut(s) 139, 445, 1273, 2131, 2606
BspDI ATCGAT 1 cut(s) 1695
BspFNI CGCG 2 cut(s) 691, 3024
BspHI TCATGA 1 cut(s) 2652
BspLI GGNNCC 7 cut(s) 6, 1677, 2277, 2344, 2560, 2915, 2916
BspMAI CTGCAG 2 cut(s) 472, 1624
BspMI ACCTGC 1 cut(s) 2065
BspOI GCTAGC 1 cut(s) 2064
BspPI GGATC 4 cut(s) 12, 1007, 3177, 3246
BspQI GCTCTTC 2 cut(s) 222, 768
BspT107I GGYRCC 2 cut(s) 1675, 2342
BspTNI GGTCTC 1 cut(s) 47
BsrDI GCAATG 3 cut(s) 2415, 2570, 3099
BsrGI TGTACA 1 cut(s) 2539
BsrI ACTGG 3 cut(s) 1981, 3056, 3215
BssECI CCNNGG 9 cut(s) 8, 60, 1050, 2197, 2481, 2562, 2661, 2926, 3186
BssMI GATC 9 cut(s) 4, 816, 1012, 2089, 2248, 2297, 3096, 3182, 3238
BssSI CACGAG 2 cut(s) 1709, 2733
BssT1I CCWWGG 6 cut(s) 60, 1050, 2197, 2481, 2562, 2926
Bst2BI CACGAG 2 cut(s) 1709, 2733
Bst2UI CCWGG 5 cut(s) 1028, 1320, 1347, 1680, 1704
Bst4CI ACNGT 9 cut(s) 412, 508, 950, 1339, 2006, 2100, 2170, 2209, 2231
Bst6I CTCTTC 7 cut(s) 222, 726, 747, 768, 789, 803, 1140
BstAUI TGTACA 1 cut(s) 2539
BstC8I GCNNGC 4 cut(s) 344, 997, 1361, 2062
BstDEI CTNAG 9 cut(s) 147, 453, 597, 882, 1260, 1392, 1444, 2118, 2593
BstDSI CCRYGG 4 cut(s) 60, 2197, 2481, 3186
BstF5I GGATG 6 cut(s) 56, 259, 1210, 1405, 1441, 1629
BstFNI CGCG 2 cut(s) 691, 3024
BstH2I RGCGCY 2 cut(s) 763, 2067
BstHHI GCGC 6 cut(s) 691, 762, 1325, 2066, 2377, 3024
BstKTI GATC 9 cut(s) 7, 819, 1015, 2092, 2251, 2300, 3099, 3185, 3241
BstMAI GTCTC 6 cut(s) 47, 252, 674, 1142, 3031, 3244
BstMBI GATC 9 cut(s) 4, 816, 1012, 2089, 2248, 2297, 3096, 3182, 3238
BstMCI CGRYCG 1 cut(s) 1415
BstMWI GCNNNNNNNGC 7 cut(s) 437, 476, 575, 1301, 1382, 2426, 2858
BstNI CCWGG 5 cut(s) 1028, 1320, 1347, 1680, 1704
BstNSI RCATGY 3 cut(s) 665, 2438, 3055
BstPAI GACNNNNGTC 1 cut(s) 1711
BstSCI CCNGG 7 cut(s) 1026, 1318, 1345, 1409, 1678, 1702, 2660
BstSFI CTRYAG 3 cut(s) 468, 519, 1620
BstUI CGCG 2 cut(s) 691, 3024
BstV1I GCAGC 6 cut(s) 865, 1076, 1360, 1487, 2359, 2380
BstV2I GAAGAC 2 cut(s) 920, 1919
BstX2I RGATCY 3 cut(s) 4, 2089, 3238
BstYI RGATCY 3 cut(s) 4, 2089, 3238
BstZI CGGCCG 1 cut(s) 1412
Bsu15I ATCGAT 1 cut(s) 1695
Bsu36I CCTNAGG 1 cut(s) 1392
BsuI GTATCC 2 cut(s) 2060, 2885
BsuRI GGCC 7 cut(s) 424, 846, 1414, 2196, 2403, 2660, 2705
BsuTUI ATCGAT 1 cut(s) 1695
BtgI CCRYGG 4 cut(s) 60, 2197, 2481, 3186
BtgZI GCGATG 1 cut(s) 2787
BtsCI GGATG 6 cut(s) 56, 259, 1210, 1405, 1441, 1629
BtsI GCAGTG 2 cut(s) 1290, 2168
BtsIMutI CAGTG 4 cut(s) 1290, 1344, 2168, 2620
BveI ACCTGC 1 cut(s) 2065
Cac8I GCNNGC 4 cut(s) 344, 997, 1361, 2062
CaiI CAGNNNCTG 2 cut(s) 425, 1319
CciI TCATGA 1 cut(s) 2652
CfoI GCGC 6 cut(s) 691, 762, 1325, 2066, 2377, 3024
Cfr13I GGNCC 8 cut(s) 422, 1641, 2276, 2401, 2444, 2559, 2630, 2914
ClaI ATCGAT 1 cut(s) 1695
CseI GACGC 1 cut(s) 3013
Csp6I GTAC 8 cut(s) 120, 1080, 1448, 2166, 2234, 2343, 2424, 2540
CspCI CAANNNNNGTGG 2 cut(s) 1749, 1784
CviQI GTAC 8 cut(s) 120, 1080, 1448, 2166, 2234, 2343, 2424, 2540
DdeI CTNAG 9 cut(s) 147, 453, 597, 882, 1260, 1392, 1444, 2118, 2593
DpnI GATC 9 cut(s) 6, 818, 1014, 2091, 2250, 2299, 3098, 3184, 3240
DpnII GATC 9 cut(s) 4, 816, 1012, 2089, 2248, 2297, 3096, 3182, 3238
DraI TTTAAA 2 cut(s) 1284, 1954
DrdI GACNNNNNNGTC 1 cut(s) 511
DseDI GACNNNNNNGTC 1 cut(s) 511
EaeI YGGCCR 1 cut(s) 1412
EagI CGGCCG 1 cut(s) 1412
Eam1104I CTCTTC 7 cut(s) 222, 726, 747, 768, 789, 803, 1140
EarI CTCTTC 7 cut(s) 222, 726, 747, 768, 789, 803, 1140
EclXI CGGCCG 1 cut(s) 1412
Eco130I CCWWGG 6 cut(s) 60, 1050, 2197, 2481, 2562, 2926
Eco147I AGGCCT 1 cut(s) 846
Eco24I GRGCYC 1 cut(s) 2119
Eco31I GGTCTC 1 cut(s) 47
Eco32I GATATC 1 cut(s) 2650
Eco47I GGWCC 6 cut(s) 1641, 2276, 2444, 2559, 2630, 2914
Eco47III AGCGCT 1 cut(s) 2065
Eco52I CGGCCG 1 cut(s) 1412
Eco57I CTGAAG 4 cut(s) 447, 726, 813, 2864
Eco81I CCTNAGG 1 cut(s) 1392
Eco88I CYCGRG 1 cut(s) 2576
EcoO109I RGGNCCY 5 cut(s) 422, 2401, 2559, 2630, 2914
EcoRI GAATTC 1 cut(s) 13
EcoRII CCWGG 5 cut(s) 1026, 1318, 1345, 1678, 1702
EcoRV GATATC 1 cut(s) 2650
EcoT14I CCWWGG 6 cut(s) 60, 1050, 2197, 2481, 2562, 2926
EcoT38I GRGCYC 1 cut(s) 2119
ErhI CCWWGG 6 cut(s) 60, 1050, 2197, 2481, 2562, 2926
Esp3I CGTCTC 2 cut(s) 252, 3031
FalI AAGNNNNNCTT 6 cut(s) 556, 588, 669, 701, 1467, 1499
FaqI GGGAC 3 cut(s) 951, 2289, 2927
FauI CCCGC 2 cut(s) 191, 2325
FauNDI CATATG 1 cut(s) 2146
FbaI TGATCA 1 cut(s) 2248
FblI GTMKAC 2 cut(s) 1137, 3150
Fnu4HI GCNGC 6 cut(s) 879, 1065, 1374, 1501, 2373, 2394
FokI GGATG 6 cut(s) 43, 266, 1217, 1412, 1448, 1616
FriOI GRGCYC 1 cut(s) 2119
Fsp4HI GCNGC 6 cut(s) 879, 1065, 1374, 1501, 2373, 2394
FspBI CTAG 6 cut(s) 443, 1766, 1790, 2061, 2672, 3177
FspI TGCGCA 1 cut(s) 2376
GlaI GCGC 6 cut(s) 690, 761, 1324, 2065, 2376, 3023
GluI GCNGC 6 cut(s) 879, 1065, 1374, 1501, 2373, 2394
GsuI CTGGAG 2 cut(s) 1368, 1602
HaeII RGCGCY 2 cut(s) 763, 2067
HaeIII GGCC 7 cut(s) 424, 846, 1414, 2196, 2403, 2660, 2705
HapII CCGG 2 cut(s) 1411, 2661
HgaI GACGC 1 cut(s) 3013
HhaI GCGC 6 cut(s) 691, 762, 1325, 2066, 2377, 3024
Hin6I GCGC 6 cut(s) 689, 760, 1323, 2064, 2375, 3022
HinP1I GCGC 6 cut(s) 689, 760, 1323, 2064, 2375, 3022
HincII GTYRAC 5 cut(s) 484, 912, 2152, 2227, 2307
HindII GTYRAC 5 cut(s) 484, 912, 2152, 2227, 2307
HpaII CCGG 2 cut(s) 1411, 2661
HphI GGTGA 9 cut(s) 47, 962, 1049, 1196, 1244, 1586, 2258, 2347, 2537
Hpy99I CGWCG 2 cut(s) 107, 3233
HpyCH4III ACNGT 9 cut(s) 412, 508, 950, 1339, 2006, 2100, 2170, 2209, 2231
HpyCH4IV ACGT 2 cut(s) 964, 3192
HpyF10VI GCNNNNNNNGC 7 cut(s) 437, 476, 575, 1301, 1382, 2426, 2858
HpyF3I CTNAG 9 cut(s) 147, 453, 597, 882, 1260, 1392, 1444, 2118, 2593
HpySE526I ACGT 2 cut(s) 964, 3192
HspAI GCGC 6 cut(s) 689, 760, 1323, 2064, 2375, 3022
KflI GGGWCCC 1 cut(s) 2914
KpnI GGTACC 1 cut(s) 2346
Ksp22I TGATCA 1 cut(s) 2248
Kzo9I GATC 9 cut(s) 4, 816, 1012, 2089, 2248, 2297, 3096, 3182, 3238
LguI GCTCTTC 2 cut(s) 222, 768
LmnI GCTCC 2 cut(s) 757, 1906
Lsp1109I GCAGC 6 cut(s) 865, 1076, 1360, 1487, 2359, 2380
MaeI CTAG 6 cut(s) 443, 1766, 1790, 2061, 2672, 3177
MaeII ACGT 2 cut(s) 964, 3192
MaeIII GTNAC 8 cut(s) 94, 1650, 1751, 2268, 2284, 2335, 2810, 3167
MalI GATC 9 cut(s) 6, 818, 1014, 2091, 2250, 2299, 3098, 3184, 3240
MboI GATC 9 cut(s) 4, 816, 1012, 2089, 2248, 2297, 3096, 3182, 3238
MfeI CAATTG 1 cut(s) 2280
MflI RGATCY 3 cut(s) 4, 2089, 3238
MhlI GDGCHC 1 cut(s) 2119
MlyI GAGTC 6 cut(s) 521, 676, 1721, 1992, 2302, 2504
MmeI TCCRAC 3 cut(s) 277, 1521, 2941
MroXI GAANNNNTTC 2 cut(s) 1482, 2082
MslI CAYNNNNRTG 1 cut(s) 3144
MspA1I CMGCKG 1 cut(s) 1373
MspI CCGG 2 cut(s) 1411, 2661
MspR9I CCNGG 7 cut(s) 1028, 1320, 1347, 1411, 1680, 1704, 2662
MunI CAATTG 1 cut(s) 2280
Mva1269I GAATGC 1 cut(s) 1903
MvaI CCWGG 5 cut(s) 1028, 1320, 1347, 1680, 1704
MvnI CGCG 2 cut(s) 691, 3024
MwoI GCNNNNNNNGC 7 cut(s) 437, 476, 575, 1301, 1382, 2426, 2858
NciI CCSGG 2 cut(s) 1411, 2662
NcoI CCATGG 3 cut(s) 60, 2197, 2481
NdeI CATATG 1 cut(s) 2146
NdeII GATC 9 cut(s) 4, 816, 1012, 2089, 2248, 2297, 3096, 3182, 3238
NheI GCTAGC 1 cut(s) 2060
NlaIV GGNNCC 7 cut(s) 6, 1677, 2277, 2344, 2560, 2915, 2916
NmuCI GTSAC 5 cut(s) 94, 1751, 2268, 2335, 3167
NsbI TGCGCA 1 cut(s) 2376
NspI RCATGY 3 cut(s) 665, 2438, 3055
OliI CACNNNNGTG 1 cut(s) 3144
PaeR7I CTCGAG 1 cut(s) 2576
PagI TCATGA 1 cut(s) 2652
PceI AGGCCT 1 cut(s) 846
PciI ACATGT 1 cut(s) 661
PciSI GCTCTTC 2 cut(s) 222, 768
PctI GAATGC 1 cut(s) 1903
PdmI GAANNNNTTC 2 cut(s) 1482, 2082
PkrI GCNGC 6 cut(s) 880, 1066, 1375, 1502, 2374, 2395
PleI GAGTC 6 cut(s) 520, 676, 1720, 1992, 2302, 2503
PpsI GAGTC 6 cut(s) 520, 676, 1720, 1992, 2302, 2503
PpuMI RGGWCCY 3 cut(s) 2559, 2630, 2914
PscI ACATGT 1 cut(s) 661
PshAI GACNNNNGTC 1 cut(s) 1711
Psp5II RGGWCCY 3 cut(s) 2559, 2630, 2914
Psp6I CCWGG 5 cut(s) 1026, 1318, 1345, 1678, 1702
PspGI CCWGG 5 cut(s) 1026, 1318, 1345, 1678, 1702
PspN4I GGNNCC 7 cut(s) 6, 1677, 2277, 2344, 2560, 2915, 2916
PspPI GGNCC 8 cut(s) 422, 1641, 2276, 2401, 2444, 2559, 2630, 2914
PspPPI RGGWCCY 3 cut(s) 2559, 2630, 2914
PspXI VCTCGAGB 1 cut(s) 2576
PstI CTGCAG 2 cut(s) 472, 1624
PstNI CAGNNNCTG 2 cut(s) 425, 1319
PsuI RGATCY 3 cut(s) 4, 2089, 3238
PvuII CAGCTG 1 cut(s) 1373
RsaI GTAC 8 cut(s) 121, 1081, 1449, 2167, 2235, 2344, 2425, 2541
RsaNI GTAC 8 cut(s) 120, 1080, 1448, 2166, 2234, 2343, 2424, 2540
RseI CAYNNNNRTG 1 cut(s) 3144
SapI GCTCTTC 2 cut(s) 222, 768
SatI GCNGC 6 cut(s) 879, 1065, 1374, 1501, 2373, 2394
Sau3AI GATC 9 cut(s) 4, 816, 1012, 2089, 2248, 2297, 3096, 3182, 3238
Sau96I GGNCC 8 cut(s) 422, 1641, 2276, 2401, 2444, 2559, 2630, 2914
ScaI AGTACT 1 cut(s) 1449
SchI GAGTC 6 cut(s) 521, 676, 1721, 1992, 2302, 2504
ScrFI CCNGG 7 cut(s) 1028, 1320, 1347, 1411, 1680, 1704, 2662
SduI GDGCHC 1 cut(s) 2119
SfcI CTRYAG 3 cut(s) 468, 519, 1620
Sfr274I CTCGAG 1 cut(s) 2576
SinI GGWCC 6 cut(s) 1641, 2276, 2444, 2559, 2630, 2914
SlaI CTCGAG 1 cut(s) 2576
SmiMI CAYNNNNRTG 1 cut(s) 3144
SmlI CTYRAG 3 cut(s) 1670, 2174, 2576
SmoI CTYRAG 3 cut(s) 1670, 2174, 2576
SseBI AGGCCT 1 cut(s) 846
SsiI CCGC 8 cut(s) 198, 374, 431, 641, 691, 1729, 2332, 2509
SspMI CTAG 6 cut(s) 443, 1766, 1790, 2061, 2672, 3177
StuI AGGCCT 1 cut(s) 846
StyD4I CCNGG 7 cut(s) 1026, 1318, 1345, 1409, 1678, 1702, 2660
StyI CCWWGG 6 cut(s) 60, 1050, 2197, 2481, 2562, 2926
TaaI ACNGT 9 cut(s) 412, 508, 950, 1339, 2006, 2100, 2170, 2209, 2231
TaiI ACGT 2 cut(s) 967, 3195
TaqI TCGA 9 cut(s) 17, 188, 222, 667, 1128, 1695, 2577, 2796, 3181
TatI WGTACW 4 cut(s) 1079, 1447, 2233, 2539
TscAI CASTG 4 cut(s) 1297, 1344, 2168, 2620
TseFI GTSAC 5 cut(s) 94, 1751, 2268, 2335, 3167
TseI GCWGC 6 cut(s) 878, 1064, 1373, 1500, 2372, 2393
Tsp45I GTSAC 5 cut(s) 94, 1751, 2268, 2335, 3167
TspDTI ATGAA 8 cut(s) 1086, 1170, 1452, 1470, 1834, 2336, 2519, 2669
TspGWI ACGGA 4 cut(s) 507, 1622, 2228, 3175
TspRI CASTG 4 cut(s) 1297, 1344, 2168, 2620
VpaK11BI GGWCC 6 cut(s) 1641, 2276, 2444, 2559, 2630, 2914
XapI RAATTY 3 cut(s) 13, 1744, 1949
XbaI TCTAGA 1 cut(s) 1789
XceI RCATGY 3 cut(s) 665, 2438, 3055
XhoI CTCGAG 1 cut(s) 2576
XmiI GTMKAC 2 cut(s) 1137, 3150
XmnI GAANNNNTTC 2 cut(s) 1482, 2082
XspI CTAG 6 cut(s) 443, 1766, 1790, 2061, 2672, 3177
ZrmI AGTACT 1 cut(s) 1449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.