Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0002582

Physical Location & Seq

Genomic Coordinates Forward (+)

35852270 .. 35859833

Gene Structure 7564 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G018100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4152 bp

ATGGGTCGGAAGAAGCCGACTGCTCGTGACGAAGAGAATCCGCCGCCAGCGGGTGGTGGTGGAGGAAAATCTAAGAAGAAGAACCTGGTGATCGAAGACGACGAGTATTCCGTCGCAACTGAGCTATCCGAGGAGAGTCAAGTCAAGGAAGAGAAGGTTCCTCCGCCTAGTAAAGGGAAGAAGGGCAAGAAGGGGAGTTCTAAAGCTTCTAAGGGAGTGGATGAGGACTTGGACGATGCCGACGAGGATGCAGATGAGGTTCCCCAAGTTGTTTTCACTGGGAAGAAGAAGGGCAAGTCGAAGAAGAGTGGCGGGAGTAGCGCGTTCAGCTCGTCCAGTTTCGGGTTGCTTGAGGATGAGGATGATAAGGATGCTGAGGATGATGAGCAGTCTGGTTTGACAGGAGATGATGAGGAGGAGGATGCTCCGGTGTTGAAATTTTCGGGGAAGAAGAAGCCTTCAAAGTCGACCAAGAAAACCGGGGCTAGTTCGTTTACAGGGTCGGCTTTTGATGCCATTGGGGACGAAGGGGATAGTGATGGCGCGGTTGATGATGAGAGTAATGCAGCTGATGATCATGAGAATGACGATGAGCCTGCAATTTCTTTTACAGGGAAAAAGAAGGCTTCCAAGGGTGGAAAGAAGGGTGGGAGTGTGTTTGCAGCAGCCAGCTTTAGTGCCCTTGATGATGCGGAGGAGGATAAGGATGAAAAAAACGATGAGGATGACGATATTCCTCAGATTACTTTCTCTGGTAAGAAAAAGAAGTCATCAAAGGCTTCAAAGAAGAGTGGCGGTAATTCTTTTAGCGCAGCATTGCTTGATGAGGAAGATGATGAAGATACTTCCGTTTCCAAACCAACTAGTGCCGGTGATGATACAGTTGAGGATGAAGATGCATCTATGATAACATTCACAGGTAAGAAGAAGTCTTCAAAGAAGAAAGGTAATAGTGTGTTTACTGCACTGGGTGAAGATGCTGGGGTTGGGAGTGAAATTACAGATGTAGTTGAGCCCAAACAACCAACTACGGGAACTAGTAAAATTGAAGCTGATAATTCTAACGTTAGTAAGAATAAAGATCCAGAACCTTCAAAAAGTAAGAAAAAGAAGAAGAAGAGTGGAAGGACTGCTCAAGAAGAGGATGATTTGGATAAGATTCTTGCAGAGCTAGGTGAGCTTTCGTCGAAACCTGCTGCTCCTCAGAAAGAGGAGACGGTGGAGGTTCAGCCTGCTGATTCTCAGAAAGAGGAGAAAGTTGTTGAGGTTCAACCTGATGCGGTTGCCCCAGTTGATGGTTCTGCTGCAAAGGAAGCTGAAGAAGAGACTGTGGAGTCTGCTGCAGCAAAGAAGAAGAAAAAGAAGAAGGAAAAGGAAAAGGAGAAAAAGGCGGCAGCAGCAGCAGCATCTGTAGGTGTCAAGGAGGAAAAACAAGAACAAGCAAAAATTGAGCCAGCAATTGAACCAAAGAAGAAGGAAGTGAAAGGCAAAGCAGCTGAAAAGAAGGTGCCTAAACATGTTAGGGAGATGCAAGAGGCCCTTGCTAGGCGACAGGAAGAAGAAGAGAAGAAGAAAAGGGAAGAAGAGGAGAAACTGAAGAAAGAAGAAGAGGAGAGGCATAAGCAGGAAGAACTTGAGAAGCAAAAGGAGGAGGCAAAGCGGAAGAAAAAGGAGAGGGAAAAGGAGAAGATCCAAGAGTTGAAGAAGGCAGGTTTATATAAAACTCCCAAGCAAAAAGAAGAGGAGAAGCGGCGGGAGCAAAAGAGACTCCAGTTACTTAGGGATTTACCTAATGCAGTGGAGGGTGTGCCTCCTCCTACCACAGACAGTGAAAAACCGGCTAAAAGACCCTTGTATCAGAAAAAGAAGTCAAAACCAGCACCGAATCAGGTAAATGGTGCAGCTACTACGAAGCCTGTGGAAAGCACAGAAGGAATAAAAGAAGAAAACCAGCAGGTTACTGTTCCTGAGCTGGATTCTGTGGGACTTGAGAAGGATGAAGAGCAGGAATCAGTTGAAGTAGCAGAAAAGCTATCTGAAGTTTCTGAATCTGTTAAAGAGAATGGATTGGAACAAGAGGAGGATGATGGTGATGATGATGAATGGGATGCAAAGAGCTGGGATGATGATGCTGTTGTTAATCTTTCATTTAAACGTGGGTTTTCTGATGAAGAGGTTGAACCTGTTGTGAATAAAGGGATGAAGCCTGCAGTGCAGAAAACCGTTCCTTCTCAACCAATAAAACCTCATGATGTTGAGAATCAGAAAAAACAAGCTGAGGTGGAAGTTGACAAAGGTAGGAAGAAAGATGCTACTGCAAAGAAAGAAGCACCAAATTCAGATTCTGCCTCTAAACAGAGTGAAGATAATCTTCGCTCTCCCATTTGCTGCATTATGGGCCATGTTGATACTGGTAAAACTAAGCTTCTGGATTGTATCCGAGGCACAAATGTTCAGGAGGGTGAGGCTGGAGGTATTACTCAACAGATTGGTGCTACGTATTTTCCTGCGGATAATATCCGGGAGAGGACTAAAGAACTGAAAGCTGATGCAAAGCTGAAGGTCCCAGGTCTATTGGTCATTGATACCCCTGGCCACGAGTCATTTACTAATTTGAGGTCACGGGGTTCAGGTTTATGTGATATTGCAATTTTGGTTGTTGACATTATGCATGGCTTAGAGCCTCAAACAATAGAGTCACTCAATCTCTTGAAAATGAGGAATACCGAATTCATTGTTGCATTGAATAAGGTGGACAGGCTCTATGGGTGGAAAACCTGCCGCAACCAGCCAATAGGGAAGGCAATGAAGCAACAATCCAAGGATGTAGTAAATGAGTTCAATATGAGGGTTTCTCAGATTATCACTCAATTCATGGAGCAGGGCTTAAATACTGAATTGTATTATAAGAATAAAGAAATGGGAGAAACATACAGTATTGTACCTACAAGTGCCATTAGTGGTGAAGGGATTCCAGATTTGTTATTGCTATTGGTTCAGTGGGCCCAGAAAACTATGGTTGAGAAACTTACTTACAGCAATGAAATTCAGTGTACGGTATTGGAGGTCAAGGTTATTGAAGGTCTTGGGACAACAATCGATGTTGTGTTGGTTAATGGTGTGCTTCATGAAGGGGATCAAATAGTCGTTTGCGGCATGCAGGGACCTATTGTTGCTTCAATTCGAGCTTTGTTGACACCACATCCCATGAAAGAACTTCGTGTCAAGGGAACTTATCAGCACCATAGTCAAATCAAAGCTGCACAAGGTATCAAGATCACAGCACAGGGACTTGAACATGCTATTGCTGGCACTGCTCTATATGTTGTTGGGCCTAGCGATGACTTGGATGATGTCAAGGAAACAGCTATGGAAGATATGAAATCAGTCATGAATAGGATTGACAAGAGTGGTGAGGGAGTTTGTGTACAAGCATCTACTCTAGGATCCTTGGAAGCATTGCTAGAGTTTTTGAAGACGCCAGAAGTTAATATTCCTGTTAGTGGTATTAGCATCGGCCCTGTACACAAGAAGGATGTCATGAAGGCCAGTGTAATGCTTGAAAAGAAAAAAGAGTACGCCACTATCTTGGCATTTGATGTTAAAGTGACACCTGAGGCCCGGGACCTTGCAGATGAATTGGGTGTGAAGATTTTTATTGCTGATATAATCTATCACTTATTTGACCAATTTAAGGCCTATATCGACAATCTCAAAGAGGAAAAGAAGAAGGAAGCCGCTGATGAAGCTGTTTTTCCGTGTGTGCTCAAGATTTTACCCAACTGTGTTTTCAACAAGAAGGATCCAATTGTGTTGGGTGTTGATGTTCTTGAAGGCATTGCAAAGGTAGGGACTCCAATTTGCATTCCTCAGAGAGATTTTATTGATATTGGACGCATTGCATCCATTGAGAATAACCACAAACCTGTAGATATAGCGAAAAAGGGGCTGAAGGTGGCTATCAAGATAGTCGGCACAAATTCTGATGAGCAGCAAAAGATGTATGGTAGGCATTTTGAAATTGAAGATGAGCTTGTCAGCCATATCTCCAGGAGGTCAATTGATGTACTTAAAACAAGCTACCGGGATGAACTGTCAATTGACGAGTGGAAGCTGGTTGTGAAATTGAAGAAACTTTTTGAGATACCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001882 GO:0001883 GO:0002181 GO:0002183 GO:0003674 GO:0003676 GO:0003723 GO:0003743 GO:0003824 GO:0003924 GO:0005488 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006412 GO:0006413 GO:0006518 GO:0006807 GO:0008135 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019001 GO:0019538 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032991 GO:0033290 GO:0034641 GO:0034645 GO:0035639 GO:0036094 GO:0043043 GO:0043167 GO:0043168 GO:0043170 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0070993 GO:0071704 GO:0097159 GO:0097367 GO:1901265 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko00195 ko01100 ko03013 map00195 map01100 map03013

Pfam Domains

Protein Families

GTP_EFTU GTP_EFTU_D2 IF-2

Protein Analysis

1383

Amino Acids

151.75

Weight (kDa)

5.23

Isoelectric Point (pI)

40.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GTP_EFTU	PF00009	794 - 1003	4.3e-34	Elongation factor Tu GTP binding domain
IF-2	PF11987	1120 - 1232	3e-23	Translation-initiation factor 2
GTP_EFTU_D4	PF14578	1253 - 1326	1.5e-14	Elongation factor Tu domain 4

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002582)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G21160 AT1G76720 AT1G76720 AT1G76810 AT2G27700 AT2G27700
fragaria_vesca	FvH4_2g13940 FvH4_2g13940 FvH4_2g13940 FvH4_4g36100 FvH4_4g36312
malus_domestica	MD05G1023200.v1.1 MD09G1087100.v1.1 MD10G1024100.v1.1
prunus_persica	Prupe.2G099300_v2.0.a1 Prupe.8G027900_v2.0.a1
pyrus_communis	pycom05g01470 pycom09g01240 pycom10g01600
rosa_chinensis	RchiOBHm_Chr4g0445721 RchiOBHm_Chr6g0275701
rosa_laevigata	RLG00000005714 RLG00000013337 RLG00000013444
rosa_multiflora	Rmu_co8469881.1_g000001 Rmu_sc0003637.1_g000011 Rmu_sc0003637.1_g000012 Rmu_sc0007122.1_g000003
rosa_roxburghii	Rroxscaffold_7G00193000
rosa_rugosa	Rorug04G0365200 Rorug06G0095700
rosa_samantha	Rh4AG426100 Rh4CG453100 Rh4DG434300 Rh6AG207600 Rh6BG210300 Rh6CG214100 Rh6DG203400
rosa_wichuraiana	Rw4G036490 Rw6G018100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2907
AasI	GACNNNNNNGTC	1 cut(s)	1333
Acc36I	ACCTGC	4 cut(s)	1201, 1700, 1945, 2786
AccB1I	GGYRCC	1 cut(s)	1507
AccB7I	CCANNNNNTGG	2 cut(s)	53, 1295
AccI	GTMKAC	1 cut(s)	467
AccII	CGCG	2 cut(s)	323, 545
AclI	AACGTT	1 cut(s)	1065
AclWI	GGATC	7 cut(s)	1076, 1684, 3144, 3441, 3454, 3797, 3810
AcoI	YGGCCR	1 cut(s)	2593
AcsI	RAATTY	5 cut(s)	437, 2335, 2729, 3045, 3979
AcuI	CTGAAG	5 cut(s)	1338, 1616, 2058, 2579, 3971
AcyI	GRCGYC	1 cut(s)	3479
AdeI	CACNNNGTG	1 cut(s)	971
AfaI	GTAC	6 cut(s)	2943, 3055, 3429, 3525, 3578, 4068
AfiI	CCNNNNNNNGG	9 cut(s)	50, 53, 173, 342, 1031, 1295, 1545, 3503, 3694
AflIII	ACRYGT	1 cut(s)	1516
AhlI	ACTAGT	2 cut(s)	863, 1037
AjnI	CCWGG	4 cut(s)	84, 2566, 2590, 4049
AjuI	GAANNNNNNNTTGG	4 cut(s)	2326, 2358, 3773, 3805
Alw21I	GWGCWC	1 cut(s)	3768
Alw26I	GTCTC	3 cut(s)	1208, 1319, 1759
AlwI	GGATC	7 cut(s)	1076, 1684, 3144, 3441, 3454, 3797, 3810
AlwNI	CAGNNNCTG	2 cut(s)	1409, 2345
Ama87I	CYCGRG	1 cut(s)	3621
AoxI	GGCC	9 cut(s)	1536, 2398, 2593, 3003, 3332, 3517, 3546, 3618, 3696
ApaI	GGGCCC	1 cut(s)	3007
ApoI	RAATTY	5 cut(s)	437, 2335, 2729, 3045, 3979
Asp700I	GAANNNNTTC	1 cut(s)	2970
AspLEI	GCGC	3 cut(s)	323, 545, 812
AsuC2I	CCSGG	5 cut(s)	481, 2522, 3622, 3623, 4085
AsuHPI	GGTGA	8 cut(s)	100, 884, 983, 1187, 2102, 2474, 2975, 3425
AvaI	CYCGRG	1 cut(s)	3621
AvaII	GGWCC	3 cut(s)	2563, 3164, 3625
AxyI	CCTNAGG	1 cut(s)	3615
BaeGI	GKGCMC	2 cut(s)	682, 3007
BaeI	ACNNNNGTAYC	4 cut(s)	834, 867, 1838, 1871
BalI	TGGCCA	1 cut(s)	2595
BamHI	GGATCC	2 cut(s)	3446, 3802
BanI	GGYRCC	1 cut(s)	1507
BanII	GRGCYC	2 cut(s)	1017, 3007
BauI	CACGAG	2 cut(s)	24, 2597
BbsI	GAAGAC	3 cut(s)	102, 924, 3482
Bbv12I	GWGCWC	1 cut(s)	3768
BbvCI	CCTCAGC	2 cut(s)	375, 2277
BccI	CCATC	3 cut(s)	533, 1289, 2081
BcgI	CGANNNNNNTGC	4 cut(s)	230, 264, 578, 612
BciT130I	CCWGG	4 cut(s)	86, 2568, 2592, 4051
BciVI	GTATCC	1 cut(s)	2447
BclI	TGATCA	1 cut(s)	574
BcnI	CCSGG	5 cut(s)	481, 2522, 3622, 3623, 4085
BcoDI	GTCTC	3 cut(s)	1208, 1319, 1759
BcuI	ACTAGT	2 cut(s)	863, 1037
BfaI	CTAG	9 cut(s)	168, 486, 864, 1038, 1172, 1545, 3336, 3443, 3464
BfmI	CTRYAG	4 cut(s)	1341, 1410, 2208, 3927
BfuAI	ACCTGC	4 cut(s)	1201, 1700, 1945, 2786
BfuI	GTATCC	1 cut(s)	2447
Bme1390I	CCNGG	9 cut(s)	86, 481, 2522, 2568, 2592, 3622, 3623, 4051, 4085
Bme18I	GGWCC	3 cut(s)	2563, 3164, 3625
BmeT110I	CYCGRG	1 cut(s)	3621
BmiI	GGNNCC	9 cut(s)	159, 261, 1509, 2565, 3005, 3165, 3448, 3626, 3804
BmrFI	CCNGG	9 cut(s)	86, 481, 2522, 2568, 2592, 3622, 3623, 4051, 4085
BmrI	ACTGGG	3 cut(s)	288, 977, 1283
BmuI	ACTGGG	3 cut(s)	288, 977, 1283
BpiI	GAAGAC	3 cut(s)	102, 924, 3482
BplI	GAGNNNNNCTC	2 cut(s)	2839, 2871
BpmI	CTGGAG	3 cut(s)	1754, 2490, 4033
Bpu10I	CCTNAGC	3 cut(s)	375, 1968, 2277
BpuEI	CTTGAG	5 cut(s)	371, 1119, 1655, 2009, 3752
BpuMI	CCSGG	5 cut(s)	481, 2522, 3622, 3623, 4085
Bsa29I	ATCGAT	1 cut(s)	3099
BsaAI	YACGTR	1 cut(s)	2499
BsaHI	GRCGYC	1 cut(s)	3479
BsaJI	CCNNGG	9 cut(s)	129, 480, 630, 2440, 2566, 2590, 2820, 3450, 3621
BsaWI	WCCGGW	1 cut(s)	427
BsaXI	ACNNNNNCTCC	2 cut(s)	3411, 3441
Bsc4I	CCNNNNNNNGG	9 cut(s)	50, 53, 173, 342, 1031, 1295, 1545, 3503, 3694
Bse118I	RCCGGY	2 cut(s)	869, 1837
Bse1I	ACTGG	7 cut(s)	283, 336, 972, 1289, 1771, 2416, 3549
Bse21I	CCTNAGG	1 cut(s)	3615
Bse3DI	GCAATG	6 cut(s)	815, 2811, 3046, 3458, 3837, 3897
BseBI	CCWGG	4 cut(s)	86, 2568, 2592, 4051
BseCI	ATCGAT	1 cut(s)	3099
BseDI	CCNNGG	9 cut(s)	129, 480, 630, 2440, 2566, 2590, 2820, 3450, 3621
BseLI	CCNNNNNNNGG	9 cut(s)	50, 53, 173, 342, 1031, 1295, 1545, 3503, 3694
BseMI	GCAATG	6 cut(s)	815, 2811, 3046, 3458, 3837, 3897
BseNI	ACTGG	7 cut(s)	283, 336, 972, 1289, 1771, 2416, 3549
BseSI	GKGCMC	2 cut(s)	682, 3007
BseYI	CCCAGC	2 cut(s)	980, 2118
BsgI	GTGCAG	4 cut(s)	948, 1920, 2234, 3246
Bsh1236I	CGCG	2 cut(s)	323, 545
BshFI	GGCC	9 cut(s)	1538, 2400, 2595, 3005, 3334, 3519, 3548, 3620, 3698
BshNI	GGYRCC	1 cut(s)	1507
BshVI	ATCGAT	1 cut(s)	3099
BsiHKAI	GWGCWC	1 cut(s)	3768
BsiHKCI	CYCGRG	1 cut(s)	3621
BsiSI	CCGG	7 cut(s)	428, 480, 870, 1838, 2521, 3622, 4084
BslFI	GGGAC	8 cut(s)	536, 1998, 2549, 3103, 3177, 3303, 3638, 3865
BslI	CCNNNNNNNGG	9 cut(s)	50, 53, 173, 342, 1031, 1295, 1545, 3503, 3694
BsmAI	GTCTC	3 cut(s)	1208, 1319, 1759
BsmBI	CGTCTC	1 cut(s)	1208
BsmFI	GGGAC	8 cut(s)	536, 1998, 2549, 3103, 3177, 3303, 3638, 3865
BsmI	GAATGC	1 cut(s)	3864
BsnI	GGCC	9 cut(s)	1538, 2400, 2595, 3005, 3334, 3519, 3548, 3620, 3698
BsoBI	CYCGRG	1 cut(s)	3621
Bsp120I	GGGCCC	1 cut(s)	3003
Bsp1286I	GDGCHC	4 cut(s)	682, 1017, 3007, 3768
Bsp1407I	TGTACA	2 cut(s)	3427, 3523
Bsp143I	GATC	8 cut(s)	90, 574, 1081, 1689, 3136, 3276, 3446, 3802
BspANI	GGCC	9 cut(s)	1538, 2400, 2595, 3005, 3334, 3519, 3548, 3620, 3698
BspDI	ATCGAT	1 cut(s)	3099
BspFNI	CGCG	2 cut(s)	323, 545
BspHI	TCATGA	5 cut(s)	577, 2248, 3127, 3390, 3540
BspLI	GGNNCC	9 cut(s)	159, 261, 1509, 2565, 3005, 3165, 3448, 3626, 3804
BspMAI	CTGCAG	2 cut(s)	1345, 2212
BspMI	ACCTGC	4 cut(s)	1201, 1700, 1945, 2786
BspPI	GGATC	7 cut(s)	1076, 1684, 3144, 3441, 3454, 3797, 3810
BspQI	GCTCTTC	1 cut(s)	1995
BspT107I	GGYRCC	1 cut(s)	1507
BsrDI	GCAATG	6 cut(s)	815, 2811, 3046, 3458, 3837, 3897
BsrFI	RCCGGY	2 cut(s)	869, 1837
BsrGI	TGTACA	2 cut(s)	3427, 3523
BsrI	ACTGG	7 cut(s)	283, 336, 972, 1289, 1771, 2416, 3549
BssAI	RCCGGY	2 cut(s)	869, 1837
BssECI	CCNNGG	9 cut(s)	129, 480, 630, 2440, 2566, 2590, 2820, 3450, 3621
BssMI	GATC	8 cut(s)	90, 574, 1081, 1689, 3136, 3276, 3446, 3802
BssNI	GRCGYC	1 cut(s)	3479
BssSI	CACGAG	2 cut(s)	24, 2597
BssT1I	CCWWGG	3 cut(s)	630, 2820, 3450
Bst2BI	CACGAG	2 cut(s)	24, 2597
Bst2UI	CCWGG	4 cut(s)	86, 2568, 2592, 4051
BstACI	GRCGYC	1 cut(s)	3479
BstAUI	TGTACA	2 cut(s)	3427, 3523
BstBAI	YACGTR	1 cut(s)	2499
BstC8I	GCNNGC	8 cut(s)	48, 597, 670, 1233, 1455, 2208, 3158, 3310
BstENI	CCTNNNNNAGG	1 cut(s)	171
BstFNI	CGCG	2 cut(s)	323, 545
BstHHI	GCGC	3 cut(s)	323, 545, 812
BstKTI	GATC	8 cut(s)	93, 577, 1084, 1692, 3139, 3279, 3449, 3805
BstMAI	GTCTC	3 cut(s)	1208, 1319, 1759
BstMBI	GATC	8 cut(s)	90, 574, 1081, 1689, 3136, 3276, 3446, 3802
BstNI	CCWGG	4 cut(s)	86, 2568, 2592, 4051
BstNSI	RCATGY	3 cut(s)	1520, 3160, 3302
BstSCI	CCNGG	9 cut(s)	84, 479, 2520, 2566, 2590, 3620, 3621, 4049, 4083
BstSFI	CTRYAG	4 cut(s)	1341, 1410, 2208, 3927
BstSLI	GKGCMC	2 cut(s)	682, 3007
BstSNI	TACGTA	1 cut(s)	2499
BstUI	CGCG	2 cut(s)	323, 545
BstV2I	GAAGAC	3 cut(s)	102, 924, 3482
BstX2I	RGATCY	4 cut(s)	1081, 1689, 3446, 3802
BstXI	CCANNNNNNTGG	1 cut(s)	3589
BstYI	RGATCY	4 cut(s)	1081, 1689, 3446, 3802
Bsu15I	ATCGAT	1 cut(s)	3099
Bsu36I	CCTNAGG	1 cut(s)	3615
BsuI	GTATCC	1 cut(s)	2447
BsuRI	GGCC	9 cut(s)	1538, 2400, 2595, 3005, 3334, 3519, 3548, 3620, 3698
BsuTUI	ATCGAT	1 cut(s)	3099
BtgZI	GCGATG	1 cut(s)	3354
BtsI	GCAGTG	3 cut(s)	1803, 2217, 3312
BtsIMutI	CAGTG	9 cut(s)	276, 965, 1803, 1834, 2217, 3005, 3056, 3312, 3556
BveI	ACCTGC	4 cut(s)	1201, 1700, 1945, 2786
Cac8I	GCNNGC	8 cut(s)	48, 597, 670, 1233, 1455, 2208, 3158, 3310
CaiI	CAGNNNCTG	2 cut(s)	1409, 2345
CciI	TCATGA	5 cut(s)	577, 2248, 3127, 3390, 3540
CfoI	GCGC	3 cut(s)	323, 545, 812
Cfr10I	RCCGGY	2 cut(s)	869, 1837
Cfr9I	CCCGGG	1 cut(s)	3621
ClaI	ATCGAT	1 cut(s)	3099
CseI	GACGC	2 cut(s)	3487, 3903
CsiI	ACCWGGT	1 cut(s)	84
Csp6I	GTAC	6 cut(s)	2942, 3054, 3428, 3524, 3577, 4067
CviQI	GTAC	6 cut(s)	2942, 3054, 3428, 3524, 3577, 4067
DpnI	GATC	8 cut(s)	92, 576, 1083, 1691, 3138, 3278, 3448, 3804
DpnII	GATC	8 cut(s)	90, 574, 1081, 1689, 3136, 3276, 3446, 3802
DraI	TTTAAA	1 cut(s)	2152
DraIII	CACNNNGTG	1 cut(s)	971
DrdI	GACNNNNNNGTC	1 cut(s)	1333
DseDI	GACNNNNNNGTC	1 cut(s)	1333
EaeI	YGGCCR	1 cut(s)	2593
EciI	GGCGGA	2 cut(s)	30, 153
Eco105I	TACGTA	1 cut(s)	2499
Eco130I	CCWWGG	3 cut(s)	630, 2820, 3450
Eco147I	AGGCCT	1 cut(s)	3698
Eco24I	GRGCYC	2 cut(s)	1017, 3007
Eco47I	GGWCC	3 cut(s)	2563, 3164, 3625
Eco57I	CTGAAG	5 cut(s)	1338, 1616, 2058, 2579, 3971
Eco81I	CCTNAGG	1 cut(s)	3615
Eco88I	CYCGRG	1 cut(s)	3621
EcoNI	CCTNNNNNAGG	1 cut(s)	171
EcoO109I	RGGNCCY	4 cut(s)	1537, 2563, 3164, 3625
EcoRI	GAATTC	1 cut(s)	2729
EcoRII	CCWGG	4 cut(s)	84, 2566, 2590, 4049
EcoT14I	CCWWGG	3 cut(s)	630, 2820, 3450
EcoT22I	ATGCAT	2 cut(s)	901, 2673
EcoT38I	GRGCYC	2 cut(s)	1017, 3007
ErhI	CCWWGG	3 cut(s)	630, 2820, 3450
Esp3I	CGTCTC	1 cut(s)	1208
FalI	AAGNNNNNCTT	6 cut(s)	1524, 1556, 2355, 2387, 4017, 4049
FaqI	GGGAC	8 cut(s)	536, 1998, 2549, 3103, 3177, 3303, 3638, 3865
FauI	CCCGC	3 cut(s)	43, 305, 1746
FbaI	TGATCA	1 cut(s)	574
FblI	GTMKAC	1 cut(s)	467
FriOI	GRGCYC	2 cut(s)	1017, 3007
FspBI	CTAG	9 cut(s)	168, 486, 864, 1038, 1172, 1545, 3336, 3443, 3464
GlaI	GCGC	3 cut(s)	322, 544, 811
GsaI	CCCAGC	2 cut(s)	984, 2122
GsuI	CTGGAG	3 cut(s)	1754, 2490, 4033
HaeIII	GGCC	9 cut(s)	1538, 2400, 2595, 3005, 3334, 3519, 3548, 3620, 3698
HapII	CCGG	7 cut(s)	428, 480, 870, 1838, 2521, 3622, 4084
HgaI	GACGC	2 cut(s)	3487, 3903
HhaI	GCGC	3 cut(s)	323, 545, 812
Hin1I	GRCGYC	1 cut(s)	3479
Hin6I	GCGC	3 cut(s)	321, 543, 810
HinP1I	GCGC	3 cut(s)	321, 543, 810
HincII	GTYRAC	4 cut(s)	468, 2290, 2662, 3195
HindII	GTYRAC	4 cut(s)	468, 2290, 2662, 3195
HindIII	AAGCTT	2 cut(s)	204, 2423
HpaII	CCGG	7 cut(s)	428, 480, 870, 1838, 2521, 3622, 4084
HphI	GGTGA	8 cut(s)	100, 884, 983, 1187, 2102, 2474, 2975, 3425
Hpy99I	CGWCG	4 cut(s)	104, 116, 245, 1189
HpyCH4IV	ACGT	3 cut(s)	1065, 2155, 2498
HpySE526I	ACGT	3 cut(s)	1065, 2155, 2498
Hsp92I	GRCGYC	1 cut(s)	3479
HspAI	GCGC	3 cut(s)	321, 543, 810
Ksp22I	TGATCA	1 cut(s)	574
Kzo9I	GATC	8 cut(s)	90, 574, 1081, 1689, 3136, 3276, 3446, 3802
LguI	GCTCTTC	1 cut(s)	1995
LmnI	GCTCC	4 cut(s)	430, 1204, 1756, 2878
MabI	ACCWGGT	1 cut(s)	84
MaeI	CTAG	9 cut(s)	168, 486, 864, 1038, 1172, 1545, 3336, 3443, 3464
MaeII	ACGT	3 cut(s)	1065, 2155, 2498
MaeIII	GTNAC	6 cut(s)	26, 1773, 1957, 2619, 2697, 3607
MalI	GATC	8 cut(s)	92, 576, 1083, 1691, 3138, 3278, 3448, 3804
MboI	GATC	8 cut(s)	90, 574, 1081, 1689, 3136, 3276, 3446, 3802
MfeI	CAATTG	4 cut(s)	1458, 3807, 4059, 4098
MflI	RGATCY	4 cut(s)	1081, 1689, 3446, 3802
MhlI	GDGCHC	4 cut(s)	682, 1017, 3007, 3768
MlsI	TGGCCA	1 cut(s)	2595
MluNI	TGGCCA	1 cut(s)	2595
MlyI	GAGTC	6 cut(s)	145, 1343, 1761, 2609, 2705, 3847
Mox20I	TGGCCA	1 cut(s)	2595
Mph1103I	ATGCAT	2 cut(s)	901, 2673
MroXI	GAANNNNTTC	1 cut(s)	2970
MscI	TGGCCA	1 cut(s)	2595
MseI	TTAA	9 cut(s)	2055, 2139, 2151, 2888, 3114, 3489, 3603, 3693, 4071
MslI	CAYNNNNRTG	1 cut(s)	582
Msp20I	TGGCCA	1 cut(s)	2595
MspA1I	CMGCKG	4 cut(s)	50, 569, 1496, 3740
MspI	CCGG	7 cut(s)	428, 480, 870, 1838, 2521, 3622, 4084
MspR9I	CCNGG	9 cut(s)	86, 481, 2522, 2568, 2592, 3622, 3623, 4051, 4085
MunI	CAATTG	4 cut(s)	1458, 3807, 4059, 4098
Mva1269I	GAATGC	1 cut(s)	3864
MvaI	CCWGG	4 cut(s)	86, 2568, 2592, 4051
MvnI	CGCG	2 cut(s)	323, 545
NciI	CCSGG	5 cut(s)	481, 2522, 3622, 3623, 4085
NdeII	GATC	8 cut(s)	90, 574, 1081, 1689, 3136, 3276, 3446, 3802
NlaIV	GGNNCC	9 cut(s)	159, 261, 1509, 2565, 3005, 3165, 3448, 3626, 3804
NmuCI	GTSAC	4 cut(s)	26, 2619, 2697, 3607
NsiI	ATGCAT	2 cut(s)	901, 2673
NspI	RCATGY	3 cut(s)	1520, 3160, 3302
PaeI	GCATGC	1 cut(s)	3160
PagI	TCATGA	5 cut(s)	577, 2248, 3127, 3390, 3540
PceI	AGGCCT	1 cut(s)	3698
PciI	ACATGT	1 cut(s)	1516
PciSI	GCTCTTC	1 cut(s)	1995
PcsI	WCGNNNNNNNCGW	3 cut(s)	99, 108, 240
PctI	GAATGC	1 cut(s)	3864
PdmI	GAANNNNTTC	1 cut(s)	2970
PflMI	CCANNNNNTGG	2 cut(s)	53, 1295
PfoI	TCCNGGA	2 cut(s)	2520, 4049
PleI	GAGTC	6 cut(s)	144, 1342, 1761, 2608, 2704, 3847
PpsI	GAGTC	6 cut(s)	144, 1342, 1761, 2608, 2704, 3847
Ppu21I	YACGTR	1 cut(s)	2499
PpuMI	RGGWCCY	3 cut(s)	2563, 3164, 3625
PscI	ACATGT	1 cut(s)	1516
PsiI	TTATAA	1 cut(s)	2907
Psp1406I	AACGTT	1 cut(s)	1065
Psp5II	RGGWCCY	3 cut(s)	2563, 3164, 3625
Psp6I	CCWGG	4 cut(s)	84, 2566, 2590, 4049
PspFI	CCCAGC	2 cut(s)	980, 2118
PspGI	CCWGG	4 cut(s)	84, 2566, 2590, 4049
PspN4I	GGNNCC	9 cut(s)	159, 261, 1509, 2565, 3005, 3165, 3448, 3626, 3804
PspOMI	GGGCCC	1 cut(s)	3003
PspPPI	RGGWCCY	3 cut(s)	2563, 3164, 3625
PstI	CTGCAG	2 cut(s)	1345, 2212
PstNI	CAGNNNCTG	2 cut(s)	1409, 2345
PsuI	RGATCY	4 cut(s)	1081, 1689, 3446, 3802
PvuII	CAGCTG	2 cut(s)	569, 1496
RsaI	GTAC	6 cut(s)	2943, 3055, 3429, 3525, 3578, 4068
RsaNI	GTAC	6 cut(s)	2942, 3054, 3428, 3524, 3577, 4067
RseI	CAYNNNNRTG	1 cut(s)	582
SalI	GTCGAC	1 cut(s)	466
SapI	GCTCTTC	1 cut(s)	1995
SaqAI	TTAA	9 cut(s)	2055, 2139, 2151, 2888, 3114, 3489, 3603, 3693, 4071
Sau3AI	GATC	8 cut(s)	90, 574, 1081, 1689, 3136, 3276, 3446, 3802
SchI	GAGTC	6 cut(s)	145, 1343, 1761, 2609, 2705, 3847
ScrFI	CCNGG	9 cut(s)	86, 481, 2522, 2568, 2592, 3622, 3623, 4051, 4085
SduI	GDGCHC	4 cut(s)	682, 1017, 3007, 3768
SexAI	ACCWGGT	1 cut(s)	84
SfcI	CTRYAG	4 cut(s)	1341, 1410, 2208, 3927
SinI	GGWCC	3 cut(s)	2563, 3164, 3625
SmaI	CCCGGG	1 cut(s)	3623
SmiMI	CAYNNNNRTG	1 cut(s)	582
SmlI	CTYRAG	5 cut(s)	350, 1134, 1634, 1988, 3767
SmoI	CTYRAG	5 cut(s)	350, 1134, 1634, 1988, 3767
SnaBI	TACGTA	1 cut(s)	2499
SpeI	ACTAGT	2 cut(s)	863, 1037
SphI	GCATGC	1 cut(s)	3160
SseBI	AGGCCT	1 cut(s)	3698
SspI	AATATT	1 cut(s)	3493
SspMI	CTAG	9 cut(s)	168, 486, 864, 1038, 1172, 1545, 3336, 3443, 3464
StuI	AGGCCT	1 cut(s)	3698
StyD4I	CCNGG	9 cut(s)	84, 479, 2520, 2566, 2590, 3620, 3621, 4049, 4083
StyI	CCWWGG	3 cut(s)	630, 2820, 3450
TaiI	ACGT	3 cut(s)	1068, 2158, 2501
TaqI	TCGA	7 cut(s)	93, 299, 467, 1187, 3099, 3184, 3705
TatI	WGTACW	3 cut(s)	3427, 3523, 4066
TauI	GCSGC	6 cut(s)	46, 1394, 1753, 2784, 3156, 3740
Tru1I	TTAA	9 cut(s)	2055, 2139, 2151, 2888, 3114, 3489, 3603, 3693, 4071
Tru9I	TTAA	9 cut(s)	2055, 2139, 2151, 2888, 3114, 3489, 3603, 3693, 4071
TscAI	CASTG	9 cut(s)	283, 972, 1803, 1834, 2217, 3005, 3056, 3319, 3556
TseFI	GTSAC	4 cut(s)	26, 2619, 2697, 3607
Tsp45I	GTSAC	4 cut(s)	26, 2619, 2697, 3607
TspGWI	ACGGA	3 cut(s)	100, 838, 3747
TspMI	CCCGGG	1 cut(s)	3621
TspRI	CASTG	9 cut(s)	283, 972, 1803, 1834, 2217, 3005, 3056, 3319, 3556
Van91I	CCANNNNNTGG	2 cut(s)	53, 1295
VpaK11BI	GGWCC	3 cut(s)	2563, 3164, 3625
XagI	CCTNNNNNAGG	1 cut(s)	171
XapI	RAATTY	5 cut(s)	437, 2335, 2729, 3045, 3979
XceI	RCATGY	3 cut(s)	1520, 3160, 3302
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2408
XmaI	CCCGGG	1 cut(s)	3621
XmiI	GTMKAC	1 cut(s)	467
XmnI	GAANNNNTTC	1 cut(s)	2970
XspI	CTAG	9 cut(s)	168, 486, 864, 1038, 1172, 1545, 3336, 3443, 3464
Zsp2I	ATGCAT	2 cut(s)	901, 2673

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.