Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0002582

Physical Location & Seq

Genomic Coordinates Reverse (-)

3875794 .. 3891824

Gene Structure 16031 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1023200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4122 bp

ATGGGTCGGAAGAAGCCGACTGCTCGCGACGATGAGAACCCGCAACCTGCTGCGGGTGCAAAGTCGAAGAAGAAGGCTGTGGTAATTGAAGACGATGAGTTTTCTATTGGAACTGAGCTGTCCGAGGAGTCTCAGGTGCAGGAAGAAAAAGTTGCTGTCACTGGAAAGAAGGGCAAAAAGGGTAATTCAAAAGCTTCAAAGAAGAGCAGGGATGAGGACGACCTCGACGATGTGGGAAAGGGGGACGAGGGCGAAGATGAGGTTCCCCAAGTTGCTTTCACTGGAAAGAAGAAGGGAAAGTCGAAGAAGAGTGGTGGGAATAGTGTGGTCAGCGCTTCGAGTTTTGGGCTGCTTGGAGATGAAGAAGACGGTGTTGATGATGGGAAATCTGGGTTAACTGGTAATGATGAGGAGGATTCTCCAGTGGTGAGTTTTTCTGGCAAGAAGAAGGCTTCGACATCGTCAAAGAAAACTGGTGGTAGTTTGTTTACTGGGTCCCCTTTTGATGCTATTGGTGATGAAGGTGATAGTGATGGTGAGGTTGTTGATGGTTCTGTGGGTAAGAGTAAAGAATATGACGATAGTGAGAATGAAGATGAGCCTGTAATTTCTTTTACGGGGAAGAAGAAGCCATCCAAGGGTGGAAAGAAGGGCAGGAGTGTGTTTGCAGCAGCTAGCTTTGATGTTCTCAATGATGCAGATGAGGATAAGGATGAAAAGAAAGATGAGGACGATGAAGTTCTTCAGATTACATTCTCCGGTAAGAAAAAGAAATCATCAAAGGGTTCAAAGAAGAGCGGTGGTAATTCTTTTAGCTCAGCCCTGCTTGATGAGGGGAATGATGAAGATACTTCAGTATCCGAACCAACTAGAGTTGGTGATGACACAGTTGATGATGAAGATGAATCTGTGATTGCGTTCACAGGTAAAAAGAAGTCTTCCAAAAAGAAAGGTAATGATGTTTTTACTGTATTGAGTGAAGATACAGATGTAATTGAACCGGAACAACCAAGTGTCGAAACTAATAAAAATGAAGCTGATGATTCTAAAACTAACAAGAGTAAAGAGGTTCCAGAAACTTCAAAAAGTAAGAAAAAGAAGAAGAAGAGTGGACGGACTGCTCAAGAAGAAGATGACTTGGATAAAATTCTTGCTGAGCTAGGTGAGGGTCCTACAACATTGACACCAGCTGAGGCTACCATGAAAGAGGAGAAAGTTGAGGTTCAACCTGATCCGGTTGCCCCAGTTGATGCTTCTGGTGAAAAGGAAGGTGAAGAAGAGACTGTGGAGTCTGCTGCTGCAAAGAAGAAGAGAAAGAAGAAGGAGAAGGAAAAGGAGAAAAAGTCAGCAGCGGCAGCAGGGACTGCTACTGCTCCCATGGTTCCTAAGGATGATAAACGAGAAGAAACCCAAATTGAACCCACAGACCCCAAGAAGAAAGAAGTGAAAGGTAAAGTAGCAGATAAGAAACTTCCAAAACATGTGAGGGAGATGCAAGAGGCTCTTGCTAGGCGACAGGAACAAGAAGAGAGATTGAAAAGGGAAGCAGAGGAGAAACAGAGGAAAGAAGAAGAGGAGCGGCGTAAGCAGGAAGAACTTGAGAGGGAAAAGGAGGCGGCCAGGATTCTGAAAAAGGAAAGGGAAAAGGAGAAGCTACAAAGGAAGAGACAGGAAGGCAAGATTTTAACTGCGAAGCAAAAAGAAGAACAACGCAGGCGGGAGGCAATGAGAAACCAGATACTTGCTAATGCAGGGGGTTTACCTCTCCTTACCACAGACAATGAGAAAAAGGCTAAACGGCCTATGTACCAGAAAAAGAAGACAAAAGCAGCACCCATTCATGCAAATGGTGCAGCTTCTGCGAAGCCAGTGGAAAACCTAGAAGAGGGAGACCAGCAGGACACTATCCCCGAGCCAGAGTCTGGGGAATTTGACAAGGTTGAAGATGTAGAGTCACTGGATCTGGAGGATAAATCTGAAGTTGCTGAATCTGTTAAAGAGAATGGAGTGGAAGAAGAAGAAGAAGAAGATGATGATGATGATGATCCTTGGGATGTTAAGAGCTTTGATGATGTTAACCTTTCTGTTAAACGTGGATTTTCTGATGAAGAGATTGACTCTGAGCCTGAACCTGTTGCAAAAAAAGACATAAAGAGTTCAGGTGCCAATCCATCAGTTTCTGCTCAGAAAACTGTTCCTTCTCAACCAATAAAATCTCAGGATGGTGAGGATAAGAAAAAACAACCTGATAATGACGTTGACAAAAGTAAGAAGAAAAGCGTTTCTGTTAAAAAAGAAGCGCCAACTTCTAATGCTGACCCCAAAGAGAGTGGAGATAACCTTCGTTCACCTATTTGCTGCATTATGGGCCATGTTGATACTGGTAAAACTAAGCTGCTGGATTGTATCAGAGGCACAAATGTTCAGGAAGGTGAGGCTGGAGGTATTACTCAGCAGATTGGAGCAACATATTTTCCTGCTGAGAACATTCGTGAGAGGACTAAAGAACTGAAAGCTGATGCGAAGCTGAAGGTCCCTGGTCTGTTGGTCATTGATACCCCCGGGCACGAGTCATTCACTAATTTACGGTCACGGGGCTCAGGTTTATGTGATATTGCTATATTGGTTGTTGACATTATGCATGGCTTAGAGCCTCAGACAATAGAGTCACTTAACCTTTTGAAAATGAGGAATACGGAATTTATTGTTGCACTAAATAAGGTGGACAGACTCTACGGGTGGAAAACCTGCCGCAATGCACCAATTGTGAAAGCAATGAAGCAACAATCTAAGGATGTACAAAATGAATTCAATATGAGGCTTGTACAGATTATCACTCAATTCAAGGAGCAGGGTTTAAATACTGAATTGTATTATAAGAATAAAGAAATGGGGGAAACCTACAGTATTATTCCTACAAGTGCCATTAGTGGTGAAGGAATTCCAGATTTGCTATTACTATTGGTTCAGTGGACACAGAAAACTATGGTTGAGAAACTCACTTTCAGCAATGAAATCCAGTGTACGGTTTTGGAGGTTAAGGTTGTGGAAGGCCTTGGGACAACAATTGATGTTGTATTGGTTAATGGTGTGCTTCATGAAGGAGATCAAATAGTGGTTTGCGGCATGCAGGGACCTATTGTTGCAACAATTCGATCTTTATTGACTCCACATCCAATGAAAGAACTTCGTGTGAAGGGAGCGTATCTGCATCATAGTGAAATCAAGGCTGCACAGGGTATTAAAATTGCAGCACAGGGTCTTGAACACGCTATTGCTGGTACTGCTCTACATGTAGTAGGGCCTCATGATGACTTGGAGGATATCAAGGAAACAGCTATGGAAGACATGAAGTCAGTTTTGAATAGAATTGACAAGACTGGTGAGGGAGTTTGCGTACAAGCATCTACTCTAGGTTCCTTGGAAGCATTGCTAGAGTTTTTGAAAACACCAGCAGTAAACATTCCTGTTAGTGGTATTAGCATAGGCCCTGTACACAAAAAGGATGTCATGAAGGCCAGTGTAATGCTTGAAAAGAAAAAGGAGTTTGCCACCATCTTGGCATTTGATGTCAAAGTTACACCGGAGGCCCGAGAAATGGCAGACGATTTAGGTGTGAAGATTTTTATTGCTGATATCATCTATCACTTGTTCGATCAATTTAAGGCATATATTGACAATATTAAGGAGGAAAAGAAGAAGGAATCTGCTGATGAAGCTGTCTTTCCTTGCGTGCTCAAGATTATGCCCAACTGTGTTTTCAACAAGAAGGATCCAATTATCTTGGGGGTTGACGTTCTTGATGGCATTTTGAAGGTGGGTACTCCAATTTGTATTCCTCAAAGGGATTTTATTTCTATTGGGCGCATTGCATCCATTGAAAATAACCACAAACCTGTTGATATGGCGAAGAAGGGGTCGAAATTAGCCATCAAGATTATTGGGTCAAATTCCGAAGAACAGCAAAAGATGTTTGGTAGGCATTTTGAGATTGATGATGAACTTGTCAGCCAAATCTCAAGGAATTCGATTGATGTACTTAAAGCAAATTACCGAGATGAACTGTCAATGGAGGAGTGGAAGCTGCTTGTGAAATTGAAGAAGCTTTTCGAGATACCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001882 GO:0001883 GO:0002181 GO:0002183 GO:0003674 GO:0003676 GO:0003723 GO:0003743 GO:0003824 GO:0003924 GO:0005488 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006412 GO:0006413 GO:0006518 GO:0006807 GO:0008135 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019001 GO:0019538 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032991 GO:0033290 GO:0034641 GO:0034645 GO:0035639 GO:0036094 GO:0043043 GO:0043167 GO:0043168 GO:0043170 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0070993 GO:0071704 GO:0097159 GO:0097367 GO:1901265 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko00195 ko01100 ko03013 map00195 map01100 map03013

Pfam Domains

Protein Families

GTP_EFTU GTP_EFTU_D2 IF-2

Protein Analysis

1374

Amino Acids

151.01

Weight (kDa)

5.24

Isoelectric Point (pI)

37.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GTP_EFTU	PF00009	784 - 993	6.7e-35	Elongation factor Tu GTP binding domain
GTP_EFTU_D2	PF03144	1020 - 1095	6.9e-07	Elongation factor Tu domain 2
IF-2	PF11987	1110 - 1222	8.2e-22	Translation-initiation factor 2
GTP_EFTU_D4	PF14578	1243 - 1335	4.6e-15	Elongation factor Tu domain 4

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002582)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G21160 AT1G76720 AT1G76720 AT1G76810 AT2G27700 AT2G27700
fragaria_vesca	FvH4_2g13940 FvH4_2g13940 FvH4_2g13940 FvH4_4g36100 FvH4_4g36312
malus_domestica	MD05G1023200.v1.1 MD09G1087100.v1.1 MD10G1024100.v1.1
prunus_persica	Prupe.2G099300_v2.0.a1 Prupe.8G027900_v2.0.a1
pyrus_communis	pycom05g01470 pycom09g01240 pycom10g01600
rosa_chinensis	RchiOBHm_Chr4g0445721 RchiOBHm_Chr6g0275701
rosa_laevigata	RLG00000005714 RLG00000013337 RLG00000013444
rosa_multiflora	Rmu_co8469881.1_g000001 Rmu_sc0003637.1_g000011 Rmu_sc0003637.1_g000012 Rmu_sc0007122.1_g000003
rosa_roxburghii	Rroxscaffold_7G00193000
rosa_rugosa	Rorug04G0365200 Rorug06G0095700
rosa_samantha	Rh4AG426100 Rh4CG453100 Rh4DG434300 Rh6AG207600 Rh6BG210300 Rh6CG214100 Rh6DG203400
rosa_wichuraiana	Rw4G036490 Rw6G018100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2877
AasI	GACNNNNNNGTC	2 cut(s)	1288, 2665
Acc36I	ACCTGC	2 cut(s)	55, 2756
AccB1I	GGYRCC	1 cut(s)	2162
AccB7I	CCANNNNNTGG	1 cut(s)	1922
AccBSI	CCGCTC	2 cut(s)	798, 1579
AccII	CGCG	1 cut(s)	27
AciI	CCGC	9 cut(s)	41, 53, 798, 1352, 1579, 1616, 1717, 2752, 3123
AclWI	GGATC	5 cut(s)	1226, 1968, 2039, 3767, 3780
AcoI	YGGCCR	1 cut(s)	1617
AcsI	RAATTY	7 cut(s)	1146, 1928, 2699, 2807, 2940, 3949, 4024
AcuI	CTGAAG	4 cut(s)	728, 837, 1998, 2549
AfaI	GTAC	9 cut(s)	1808, 2799, 2826, 3025, 3283, 3399, 3495, 3823, 4038
AfeI	AGCGCT	1 cut(s)	334
AfiI	CCNNNNNNNGG	7 cut(s)	53, 638, 1885, 1922, 3025, 3473, 3598
AflIII	ACRYGT	2 cut(s)	1480, 3292
AhdI	GACNNNNNGTC	1 cut(s)	3352
AjnI	CCWGG	2 cut(s)	1619, 2536
AjuI	GAANNNNNNNTTGG	2 cut(s)	3743, 3775
Alw21I	GWGCWC	1 cut(s)	3738
Alw26I	GTCTC	4 cut(s)	135, 1274, 1660, 1884
AlwI	GGATC	5 cut(s)	1226, 1968, 2039, 3767, 3780
AlwNI	CAGNNNCTG	4 cut(s)	1364, 1859, 1922, 2180
Ama87I	CYCGRG	3 cut(s)	1910, 2561, 3591
Aor51HI	AGCGCT	1 cut(s)	334
AoxI	GGCC	8 cut(s)	1617, 1799, 2368, 3052, 3302, 3487, 3516, 3588
ApoI	RAATTY	7 cut(s)	1146, 1928, 2699, 2807, 2940, 3949, 4024
ArsI	GACNNNNNNTTYG	4 cut(s)	3492, 3524, 3902, 3934
Asp700I	GAANNNNTTC	3 cut(s)	741, 2940, 4106
AspLEI	GCGC	3 cut(s)	335, 2302, 3867
AspS9I	GGNCC	8 cut(s)	495, 1169, 2368, 2533, 3134, 3302, 3488, 3589
AsuC2I	CCSGG	2 cut(s)	2562, 2563
AsuNHI	GCTAGC	1 cut(s)	674
AvaI	CYCGRG	3 cut(s)	1910, 2561, 3591
AvaII	GGWCC	4 cut(s)	495, 1169, 2533, 3134
AxyI	CCTNAGG	1 cut(s)	1386
BaeGI	GKGCMC	1 cut(s)	2568
BaeI	ACNNNNGTAYC	6 cut(s)	840, 840, 873, 873, 975, 1008
BamHI	GGATCC	1 cut(s)	3772
BanI	GGYRCC	1 cut(s)	2162
BanII	GRGCYC	1 cut(s)	2600
BauI	CACGAG	1 cut(s)	2567
BbsI	GAAGAC	5 cut(s)	96, 372, 930, 1826, 3351
Bbv12I	GWGCWC	1 cut(s)	3738
BbvCI	CCTCAGC	1 cut(s)	1191
BccI	CCATC	9 cut(s)	374, 527, 542, 640, 2179, 2216, 3563, 3797, 3938
BceAI	ACGGC	1 cut(s)	1814
BciT130I	CCWGG	2 cut(s)	1621, 2538
BciVI	GTATCC	1 cut(s)	868
BcnI	CCSGG	2 cut(s)	2562, 2563
BcoDI	GTCTC	4 cut(s)	135, 1274, 1660, 1884
BfaI	CTAG	7 cut(s)	675, 870, 1160, 1509, 1880, 3413, 3434
BfmI	CTRYAG	1 cut(s)	2902
BfoI	RGCGCY	2 cut(s)	336, 2303
BfuAI	ACCTGC	2 cut(s)	55, 2756
BfuI	GTATCC	1 cut(s)	868
BlpI	GCTNAGC	2 cut(s)	817, 1155
Bme1390I	CCNGG	4 cut(s)	1621, 2538, 2562, 2563
Bme18I	GGWCC	4 cut(s)	495, 1169, 2533, 3134
BmeRI	GACNNNNNGTC	1 cut(s)	3352
BmeT110I	CYCGRG	3 cut(s)	1910, 2561, 3591
BmgT120I	GGNCC	8 cut(s)	495, 1169, 2368, 2533, 3134, 3302, 3488, 3589
BmrFI	CCNGG	4 cut(s)	1621, 2538, 2562, 2563
BmrI	ACTGGG	2 cut(s)	501, 1238
BmsI	GCATC	8 cut(s)	496, 685, 1240, 1482, 2509, 3220, 3413, 3881
BmtI	GCTAGC	1 cut(s)	678
BmuI	ACTGGG	2 cut(s)	501, 1238
BpiI	GAAGAC	5 cut(s)	96, 372, 930, 1826, 3351
BplI	GAGNNNNNCTC	2 cut(s)	2102, 2134
BpmI	CTGGAG	3 cut(s)	405, 1985, 2460
Bpu10I	CCTNAGC	2 cut(s)	1191, 2599
Bpu1102I	GCTNAGC	2 cut(s)	817, 1155
BpuEI	CTTGAG	4 cut(s)	1107, 1619, 3722, 4003
BpuMI	CCSGG	2 cut(s)	2562, 2563
BsaBI	GATNNNNATC	1 cut(s)	2043
BsaI	GGTCTC	1 cut(s)	1884
BsaJI	CCNNGG	9 cut(s)	123, 636, 1377, 2048, 2536, 2560, 2561, 3055, 3420
BsaWI	WCCGGW	4 cut(s)	758, 1000, 1234, 3583
BsaXI	ACNNNNNCTCC	4 cut(s)	1328, 1358, 3381, 3411
Bsc4I	CCNNNNNNNGG	7 cut(s)	53, 638, 1885, 1922, 3025, 3473, 3598
Bse21I	CCTNAGG	1 cut(s)	1386
Bse3DI	GCAATG	6 cut(s)	1731, 2761, 2781, 3016, 3428, 3867
Bse8I	GATNNNNATC	1 cut(s)	2043
BseBI	CCWGG	2 cut(s)	1621, 2538
BseDI	CCNNGG	9 cut(s)	123, 636, 1377, 2048, 2536, 2560, 2561, 3055, 3420
BseJI	GATNNNNATC	1 cut(s)	2043
BseLI	CCNNNNNNNGG	7 cut(s)	53, 638, 1885, 1922, 3025, 3473, 3598
BseMI	GCAATG	6 cut(s)	1731, 2761, 2781, 3016, 3428, 3867
BseRI	GAGGAG	6 cut(s)	140, 425, 1223, 1565, 1589, 4088
BseSI	GKGCMC	1 cut(s)	2568
BsgI	GTGCAG	3 cut(s)	158, 1872, 3216
Bsh1236I	CGCG	1 cut(s)	27
BshFI	GGCC	8 cut(s)	1619, 1801, 2370, 3054, 3304, 3489, 3518, 3590
BshNI	GGYRCC	1 cut(s)	2162
BsiHKAI	GWGCWC	1 cut(s)	3738
BsiHKCI	CYCGRG	3 cut(s)	1910, 2561, 3591
BsiSI	CCGG	5 cut(s)	759, 1001, 1235, 2562, 3584
BslFI	GGGAC	6 cut(s)	257, 481, 1375, 2519, 3073, 3147
BslI	CCNNNNNNNGG	7 cut(s)	53, 638, 1885, 1922, 3025, 3473, 3598
BsmAI	GTCTC	4 cut(s)	135, 1274, 1660, 1884
BsmFI	GGGAC	6 cut(s)	257, 481, 1375, 2519, 3073, 3147
BsnI	GGCC	8 cut(s)	1619, 1801, 2370, 3054, 3304, 3489, 3518, 3590
Bso31I	GGTCTC	1 cut(s)	1884
BsoBI	CYCGRG	3 cut(s)	1910, 2561, 3591
Bsp1286I	GDGCHC	3 cut(s)	2568, 2600, 3738
Bsp1407I	TGTACA	3 cut(s)	2797, 2824, 3493
Bsp143I	GATC	7 cut(s)	1231, 1960, 2044, 3106, 3155, 3655, 3772
Bsp1720I	GCTNAGC	2 cut(s)	817, 1155
Bsp19I	CCATGG	1 cut(s)	1377
Bsp68I	TCGCGA	1 cut(s)	27
BspACI	CCGC	9 cut(s)	41, 53, 798, 1352, 1579, 1616, 1717, 2752, 3123
BspANI	GGCC	8 cut(s)	1619, 1801, 2370, 3054, 3304, 3489, 3518, 3590
BspFNI	CGCG	1 cut(s)	27
BspHI	TCATGA	3 cut(s)	3097, 3307, 3510
BspMI	ACCTGC	2 cut(s)	55, 2756
BspOI	GCTAGC	1 cut(s)	678
BspPI	GGATC	5 cut(s)	1226, 1968, 2039, 3767, 3780
BspQI	GCTCTTC	2 cut(s)	197, 788
BspT107I	GGYRCC	1 cut(s)	2162
BspTNI	GGTCTC	1 cut(s)	1884
BsrBI	CCGCTC	2 cut(s)	798, 1579
BsrDI	GCAATG	6 cut(s)	1731, 2761, 2781, 3016, 3428, 3867
BsrGI	TGTACA	3 cut(s)	2797, 2824, 3493
BssECI	CCNNGG	9 cut(s)	123, 636, 1377, 2048, 2536, 2560, 2561, 3055, 3420
BssMI	GATC	7 cut(s)	1231, 1960, 2044, 3106, 3155, 3655, 3772
BssSI	CACGAG	1 cut(s)	2567
BssT1I	CCWWGG	5 cut(s)	636, 1377, 2048, 3055, 3420
Bst2BI	CACGAG	1 cut(s)	2567
Bst2UI	CCWGG	2 cut(s)	1621, 2538
BstAPI	GCANNNNNTGC	3 cut(s)	1364, 1850, 1859
BstAUI	TGTACA	3 cut(s)	2797, 2824, 3493
BstC8I	GCNNGC	5 cut(s)	25, 676, 1715, 3128, 3734
BstDSI	CCRYGG	1 cut(s)	1377
BstENI	CCTNNNNNAGG	1 cut(s)	1883
BstFNI	CGCG	1 cut(s)	27
BstH2I	RGCGCY	2 cut(s)	336, 2303
BstHHI	GCGC	3 cut(s)	335, 2302, 3867
BstKTI	GATC	7 cut(s)	1234, 1963, 2047, 3109, 3158, 3658, 3775
BstMAI	GTCTC	4 cut(s)	135, 1274, 1660, 1884
BstMBI	GATC	7 cut(s)	1231, 1960, 2044, 3106, 3155, 3655, 3772
BstMWI	GCNNNNNNNGC	9 cut(s)	56, 1355, 1364, 1585, 1850, 1859, 2367, 3284, 3716
BstNI	CCWGG	2 cut(s)	1621, 2538
BstNSI	RCATGY	3 cut(s)	1484, 3130, 3296
BstSCI	CCNGG	4 cut(s)	1619, 2536, 2560, 2561
BstSFI	CTRYAG	1 cut(s)	2902
BstSLI	GKGCMC	1 cut(s)	2568
BstUI	CGCG	1 cut(s)	27
BstV2I	GAAGAC	5 cut(s)	96, 372, 930, 1826, 3351
BstX2I	RGATCY	2 cut(s)	1960, 3772
BstXI	CCANNNNNNTGG	1 cut(s)	3559
BstYI	RGATCY	2 cut(s)	1960, 3772
Bsu36I	CCTNAGG	1 cut(s)	1386
BsuI	GTATCC	1 cut(s)	868
BsuRI	GGCC	8 cut(s)	1619, 1801, 2370, 3054, 3304, 3489, 3518, 3590
BtgI	CCRYGG	1 cut(s)	1377
BtsIMutI	CAGTG	8 cut(s)	159, 279, 429, 1875, 1955, 2975, 3026, 3526
BtuMI	TCGCGA	1 cut(s)	27
BveI	ACCTGC	2 cut(s)	55, 2756
Cac8I	GCNNGC	5 cut(s)	25, 676, 1715, 3128, 3734
CaiI	CAGNNNCTG	4 cut(s)	1364, 1859, 1922, 2180
CciI	TCATGA	3 cut(s)	3097, 3307, 3510
CfoI	GCGC	3 cut(s)	335, 2302, 3867
Cfr13I	GGNCC	8 cut(s)	495, 1169, 2368, 2533, 3134, 3302, 3488, 3589
Cfr9I	CCCGGG	1 cut(s)	2561
Csp6I	GTAC	9 cut(s)	1807, 2798, 2825, 3024, 3282, 3398, 3494, 3822, 4037
CspCI	CAANNNNNGTGG	2 cut(s)	2311, 2346
CviQI	GTAC	9 cut(s)	1807, 2798, 2825, 3024, 3282, 3398, 3494, 3822, 4037
DpnI	GATC	7 cut(s)	1233, 1962, 2046, 3108, 3157, 3657, 3774
DpnII	GATC	7 cut(s)	1231, 1960, 2044, 3106, 3155, 3655, 3772
DraI	TTTAAA	1 cut(s)	2859
DrdI	GACNNNNNNGTC	2 cut(s)	1288, 2665
DriI	GACNNNNNGTC	1 cut(s)	3352
DseDI	GACNNNNNNGTC	2 cut(s)	1288, 2665
EaeI	YGGCCR	1 cut(s)	1617
Eam1105I	GACNNNNNGTC	1 cut(s)	3352
Eco130I	CCWWGG	5 cut(s)	636, 1377, 2048, 3055, 3420
Eco147I	AGGCCT	1 cut(s)	3054
Eco24I	GRGCYC	1 cut(s)	2600
Eco31I	GGTCTC	1 cut(s)	1884
Eco32I	GATATC	2 cut(s)	3325, 3637
Eco47I	GGWCC	4 cut(s)	495, 1169, 2533, 3134
Eco47III	AGCGCT	1 cut(s)	334
Eco57I	CTGAAG	4 cut(s)	728, 837, 1998, 2549
Eco81I	CCTNAGG	1 cut(s)	1386
Eco88I	CYCGRG	3 cut(s)	1910, 2561, 3591
EcoNI	CCTNNNNNAGG	1 cut(s)	1883
EcoO109I	RGGNCCY	6 cut(s)	495, 1169, 2533, 3134, 3302, 3488
EcoRI	GAATTC	3 cut(s)	2807, 2940, 4024
EcoRII	CCWGG	2 cut(s)	1619, 2536
EcoRV	GATATC	2 cut(s)	3325, 3637
EcoT14I	CCWWGG	5 cut(s)	636, 1377, 2048, 3055, 3420
EcoT22I	ATGCAT	1 cut(s)	2643
EcoT38I	GRGCYC	1 cut(s)	2600
ErhI	CCWWGG	5 cut(s)	636, 1377, 2048, 3055, 3420
FalI	AAGNNNNNCTT	2 cut(s)	1488, 1520
FaqI	GGGAC	6 cut(s)	257, 481, 1375, 2519, 3073, 3147
FauI	CCCGC	3 cut(s)	46, 48, 1710
FriOI	GRGCYC	1 cut(s)	2600
FspBI	CTAG	7 cut(s)	675, 870, 1160, 1509, 1880, 3413, 3434
GlaI	GCGC	3 cut(s)	334, 2301, 3866
GsuI	CTGGAG	3 cut(s)	405, 1985, 2460
HaeII	RGCGCY	2 cut(s)	336, 2303
HaeIII	GGCC	8 cut(s)	1619, 1801, 2370, 3054, 3304, 3489, 3518, 3590
HapII	CCGG	5 cut(s)	759, 1001, 1235, 2562, 3584
HhaI	GCGC	3 cut(s)	335, 2302, 3867
Hin6I	GCGC	3 cut(s)	333, 2300, 3865
HinP1I	GCGC	3 cut(s)	333, 2300, 3865
HincII	GTYRAC	5 cut(s)	396, 2077, 2260, 2632, 3793
HindII	GTYRAC	5 cut(s)	396, 2077, 2260, 2632, 3793
HindIII	AAGCTT	2 cut(s)	192, 4103
HpaI	GTTAAC	2 cut(s)	396, 2077
HpaII	CCGG	5 cut(s)	759, 1001, 1235, 2562, 3584
Hpy99I	CGWCG	2 cut(s)	32, 230
HpyCH4IV	ACGT	3 cut(s)	2092, 2256, 3795
HpyF10VI	GCNNNNNNNGC	9 cut(s)	56, 1355, 1364, 1585, 1850, 1859, 2367, 3284, 3716
HpySE526I	ACGT	3 cut(s)	2092, 2256, 3795
HspAI	GCGC	3 cut(s)	333, 2300, 3865
KflI	GGGWCCC	1 cut(s)	495
KspAI	GTTAAC	2 cut(s)	396, 2077
Kzo9I	GATC	7 cut(s)	1231, 1960, 2044, 3106, 3155, 3655, 3772
LguI	GCTCTTC	2 cut(s)	197, 788
LmnI	GCTCC	5 cut(s)	1378, 1576, 2462, 2848, 3200
LweI	GCATC	8 cut(s)	496, 685, 1240, 1482, 2509, 3220, 3413, 3881
MaeI	CTAG	7 cut(s)	675, 870, 1160, 1509, 1880, 3413, 3434
MaeII	ACGT	3 cut(s)	2092, 2256, 3795
MaeIII	GTNAC	5 cut(s)	157, 1953, 2589, 2667, 3577
MalI	GATC	7 cut(s)	1233, 1962, 2046, 3108, 3157, 3657, 3774
MbiI	CCGCTC	2 cut(s)	798, 1579
MboI	GATC	7 cut(s)	1231, 1960, 2044, 3106, 3155, 3655, 3772
MfeI	CAATTG	2 cut(s)	2763, 3066
MflI	RGATCY	2 cut(s)	1960, 3772
MhlI	GDGCHC	3 cut(s)	2568, 2600, 3738
MlyI	GAGTC	9 cut(s)	137, 1298, 1928, 1961, 2111, 2579, 2675, 2724, 3160
Mph1103I	ATGCAT	1 cut(s)	2643
MroXI	GAANNNNTTC	3 cut(s)	741, 2940, 4106
MslI	CAYNNNNRTG	2 cut(s)	1845, 3216
MspA1I	CMGCKG	2 cut(s)	1190, 1352
MspI	CCGG	5 cut(s)	759, 1001, 1235, 2562, 3584
MspR9I	CCNGG	4 cut(s)	1621, 2538, 2562, 2563
MunI	CAATTG	2 cut(s)	2763, 3066
MvaI	CCWGG	2 cut(s)	1621, 2538
MvnI	CGCG	1 cut(s)	27
MwoI	GCNNNNNNNGC	9 cut(s)	56, 1355, 1364, 1585, 1850, 1859, 2367, 3284, 3716
NciI	CCSGG	2 cut(s)	2562, 2563
NcoI	CCATGG	1 cut(s)	1377
NdeII	GATC	7 cut(s)	1231, 1960, 2044, 3106, 3155, 3655, 3772
NheI	GCTAGC	1 cut(s)	674
NmuCI	GTSAC	4 cut(s)	157, 1953, 2589, 2667
NruI	TCGCGA	1 cut(s)	27
NsiI	ATGCAT	1 cut(s)	2643
NspI	RCATGY	3 cut(s)	1484, 3130, 3296
PaeI	GCATGC	1 cut(s)	3130
PagI	TCATGA	3 cut(s)	3097, 3307, 3510
PceI	AGGCCT	1 cut(s)	3054
PciI	ACATGT	2 cut(s)	1480, 3292
PciSI	GCTCTTC	2 cut(s)	197, 788
PcsI	WCGNNNNNNNCGW	1 cut(s)	225
PdmI	GAANNNNTTC	3 cut(s)	741, 2940, 4106
PfeI	GAWTC	6 cut(s)	416, 905, 1043, 1624, 1988, 3704
PflFI	GACNNNGTC	1 cut(s)	460
PflMI	CCANNNNNTGG	1 cut(s)	1922
PleI	GAGTC	9 cut(s)	136, 1297, 1927, 1960, 2111, 2578, 2674, 2724, 3160
PpsI	GAGTC	9 cut(s)	136, 1297, 1927, 1960, 2111, 2578, 2674, 2724, 3160
PpuMI	RGGWCCY	4 cut(s)	495, 1169, 2533, 3134
PscI	ACATGT	2 cut(s)	1480, 3292
PsiI	TTATAA	1 cut(s)	2877
Psp5II	RGGWCCY	4 cut(s)	495, 1169, 2533, 3134
Psp6I	CCWGG	2 cut(s)	1619, 2536
PspGI	CCWGG	2 cut(s)	1619, 2536
PspPI	GGNCC	8 cut(s)	495, 1169, 2368, 2533, 3134, 3302, 3488, 3589
PspPPI	RGGWCCY	4 cut(s)	495, 1169, 2533, 3134
PstNI	CAGNNNCTG	4 cut(s)	1364, 1859, 1922, 2180
PsuI	RGATCY	2 cut(s)	1960, 3772
PsyI	GACNNNGTC	1 cut(s)	460
PvuII	CAGCTG	1 cut(s)	1190
RruI	TCGCGA	1 cut(s)	27
RsaI	GTAC	9 cut(s)	1808, 2799, 2826, 3025, 3283, 3399, 3495, 3823, 4038
RsaNI	GTAC	9 cut(s)	1807, 2798, 2825, 3024, 3282, 3398, 3494, 3822, 4037
RseI	CAYNNNNRTG	2 cut(s)	1845, 3216
SapI	GCTCTTC	2 cut(s)	197, 788
Sau3AI	GATC	7 cut(s)	1231, 1960, 2044, 3106, 3155, 3655, 3772
Sau96I	GGNCC	8 cut(s)	495, 1169, 2368, 2533, 3134, 3302, 3488, 3589
SchI	GAGTC	9 cut(s)	137, 1298, 1928, 1961, 2111, 2579, 2675, 2724, 3160
ScrFI	CCNGG	4 cut(s)	1621, 2538, 2562, 2563
SduI	GDGCHC	3 cut(s)	2568, 2600, 3738
SfaNI	GCATC	8 cut(s)	496, 685, 1240, 1482, 2509, 3220, 3413, 3881
SfcI	CTRYAG	1 cut(s)	2902
SinI	GGWCC	4 cut(s)	495, 1169, 2533, 3134
SmaI	CCCGGG	1 cut(s)	2563
SmiMI	CAYNNNNRTG	2 cut(s)	1845, 3216
SmlI	CTYRAG	4 cut(s)	1122, 1598, 3737, 4018
SmoI	CTYRAG	4 cut(s)	1122, 1598, 3737, 4018
SphI	GCATGC	1 cut(s)	3130
SseBI	AGGCCT	1 cut(s)	3054
SsiI	CCGC	9 cut(s)	41, 53, 798, 1352, 1579, 1616, 1717, 2752, 3123
SspI	AATATT	1 cut(s)	3682
SspMI	CTAG	7 cut(s)	675, 870, 1160, 1509, 1880, 3413, 3434
StuI	AGGCCT	1 cut(s)	3054
StyD4I	CCNGG	4 cut(s)	1619, 2536, 2560, 2561
StyI	CCWWGG	5 cut(s)	636, 1377, 2048, 3055, 3420
TaiI	ACGT	3 cut(s)	2095, 2259, 3798
TatI	WGTACW	4 cut(s)	2797, 2824, 3493, 4036
TauI	GCSGC	5 cut(s)	1355, 1582, 1619, 2754, 3126
TfiI	GAWTC	6 cut(s)	416, 905, 1043, 1624, 1988, 3704
TscAI	CASTG	8 cut(s)	166, 286, 429, 1875, 1962, 2975, 3026, 3526
TseFI	GTSAC	4 cut(s)	157, 1953, 2589, 2667
Tsp45I	GTSAC	4 cut(s)	157, 1953, 2589, 2667
TspGWI	ACGGA	2 cut(s)	1129, 2711
TspMI	CCCGGG	1 cut(s)	2561
TspRI	CASTG	8 cut(s)	166, 286, 429, 1875, 1962, 2975, 3026, 3526
Tth111I	GACNNNGTC	1 cut(s)	460
Van91I	CCANNNNNTGG	1 cut(s)	1922
VpaK11BI	GGWCC	4 cut(s)	495, 1169, 2533, 3134
XagI	CCTNNNNNAGG	1 cut(s)	1883
XapI	RAATTY	7 cut(s)	1146, 1928, 2699, 2807, 2940, 3949, 4024
XceI	RCATGY	3 cut(s)	1484, 3130, 3296
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2378
XmaI	CCCGGG	1 cut(s)	2561
XmnI	GAANNNNTTC	3 cut(s)	741, 2940, 4106
XspI	CTAG	7 cut(s)	675, 870, 1160, 1509, 1880, 3413, 3434
Zsp2I	ATGCAT	1 cut(s)	2643

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.