Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0002582

Physical Location & Seq

Genomic Coordinates Reverse (-)

30001325 .. 30010836

Gene Structure 9512 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000013337

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3675 bp

ATGGGTCGGAAGAAGCCGACTGCTCGTGACGAAGAGAATCCGCCACCAGCGGGTGGTGGTGGAGGAAAATCTAAGAAGAAGAACCTGGTGATCGAAGACGACGAGTATTCCGTCGCAACTGAGCTATCCGAGGAGAGTCAAGTCCAGAAAAAGAGAAGGTTCCTCCGCCTAGTAAAGGGAAGAAGGAAGAAGGGCAAGTCGAAGAAGAGTGGCAGGAGTAGCGCGTTCAGCTCGTCCAGTTTCGGGTTGTTTGAGGATGAGGATGATAAGGATGCTGAGCAATCTGGTTTAACAGGTGATGATGATGAGGAGGATGCTCCGGCGTTGAAATTTTCGGGGAAGAAGAAGCCTTCAAAGTCGTCCAAGAAAACCGGCGGTAGTTCGTTTACAGCGTCGGCTTTTGATGCCACTGGTGACGAAGGGGATAGTGATGGCGTGGTTGATGATGAGAGTAATGCAGCTGAAGATCATGAGAATGACGATGAGCCTGCAATTGCTTTTACAGGGAAGAAGAAGGCTTCCAAGGGTGGAAAGAAGGGTGGGAGTGTGTTTGCAGCAGCCAGCTTTAGTGCCCTTGATGGTGCGGAGGAGGATAAGGATGAAAAAAATGATGAGGATGACGACATTCCGCAGATTACTTTCTCTGGTAAGAAAAAGAAGTTATCAAAGGCTTCAAAGAAGAGTGGCGGTAATTCTTTTAGCGCAGCATTGCTTGATGAGGAAGACGATGAAGATACTTCGGTTTCCAAACCAACTAGTGCCGGTGATGGTACAGTTGAGGATGAAGATGCATCTATGATTACATTCACAGGTAAGAAGAAGGTCTTCAAAGAAGAAAGTAAAATTGAAGCTGATAATTCTAACGTTAGTAAGAATAAAAATCCAGAACCTTCAAAAAGTAAGAAAAAGAAGAAGAAGAGTGGAAGGACTGTTCAAGAAGACGATGATTTGGATAAGATTCTTGCAGAGCTAGAGGAGACGGTAGAGGTTCAGCCTGCTGATTCTCAGAAAGAGGAGAAAGTTGAGGTTCAACCTGATGCGGTTTCCCCAGTTGATGGTTCGGCTGCAAAGGAAGCTGAAGTAGAGACTGTGGAGTCTGCTGCAGCAAAGAAGAAGAAAAAGAAGAAGGAAAAGGAAAAGGAGAAAAAGGCGGCAGCGGCATCTGCATCTGTAGATGTCAAGGAGGAAAAACAAGAACAAGCAAAAATTGAGCCAGCAATTGAACCAAAGAAGAAGGAAGTGAAAGGCAAAGCAGCTGAAAAGAAGGTGCCTAAACATGTTAAGGAGATGCAAGAGGCCCTTGCTAGGCGACAGGAAGAAGAAGAGGGGAAGAAAAGGGAAGAAGAGGAGAAACTGAGGAAAGAAGAAGAGGAGAGGCATAAGCAGGAAGAACTTGACAAGCAAAAGGAGGAGGCAAAGCGGAAGAAAAAGGAGAGGATCCAAGAGTTGAAGAAGGCAGGTTTATATAAAACTCCCAAGCAAAAAGAAGAGGAGAAGCGGTGGGAGCAAATGAGAAACCAGTTACTTGTGAAATTACCTAATGCAGTGGAGGGTGTGCCTCCTCCTCCTACCACAGACAATGAAAAACCGGCTAAAAGACCCTTATATCAGAAAAAGAAGTCAACCAATCAGGCAAATGGTGCAGCTACTACGAAGCCTGTGGAAAACACAGAAGAAAGAAAAGAAGAAAACCAGCAAGTTACTGTTCATGAGATGGATTCTGTGGAACTCGAGAAGGTTGAAGAGCAGGAATCAGTTGAAGTAGCAAAAAAGCTATCCGGAGTTTCTGAATCTGTTAAAGAGAATGGATTGGAACAAGAAGAGGATGATGGTGATGATGATGAATGGGATGCAAAGAGCTGGGATGATGCTGCTGCTGTTAATCTTTCATTTAAATGCGGGCTTTCTGATGAAGAGGTTGAACCTGTTGTGAATAAAGGGATGAAGCCTGCAGTTTCTGAGCAGAAAACATTTCCTTCTCAACCAAGAAAACCTCATGATGTTGAGAATCAGAAAAAACAAGCTGAGGTGGAAGTTGACATAGTCACAAATGTTCAGGAGGGTGAGGCTGGAGGTATTACTCAACGGATTGGTGCTACGTATTTTCCTGCGGATAACATCCGTGAGAGGACTAAGGAACTGAAAGCTGATGCAAAGCTGAAGGTCCCAGGTCTGTTGGTCATTGATACCCCTGGGCACGAATCATTTACTAATTTAAGGTCACGGGGTTCTGGTTTATGTGATATTGCAATGTCGGTTGTTGACATTATGCATGGCTTAGAGCCTCAAACAATAGAGTCACTCAATCTCTTGAAAATGAGGAATACCGAATTCATTGTTGCATTGAATAAGGTGGACAGGCTCTATGGGTGGAAAACCTGCCGCAACCAGCCAATAGGGAAGGCAATGAAGCAACAATCCAAGGATGTAGTAAATGAGTACAATATGAGGGTTTCTCAGCAGGGCTTAAATACTGAATTGTATTATAAGAATAAAGAAATGGGAGAAACACACAGTATTGTACCTACAAGTGCCATGAGTGGTGAAGGGATTCCAGATTTGCTTTTACTATTGGTTCAGTGGGCCCAGAAAACTATGGTTGAGAAACTTACTTACAGCAATGAAATTCAGTGTACGGTAGTGGAGGTCAAGGTTATTGAAGGCCTTGGGACAACAATTGATGTTGTGTTGGTTAATGGTGTGCTTCATGAAGGAGATCAAATAGTCGTCTGCAGCATGCAGGGACCTATTGTTACTTCAACTCGAGTTTTGTTGACACCACGTCCCATGAAAGAACTTCGTATCAAGGGAACTTATCAGCACCATAGTCAAATCAAAGCTGCACAAGGTATCAAAATCACAGCACAGGGACTTGAACATGCTATTGCCGGCACTGCTCTATATGTTGTTGGGCCTCGTGATGACTCGGATGATATCAAGGAATCAGCTATGGAAGATATGAAATCAGTCCTGAATAGGATTGACAAGAGTGGTGAGGGAGTTTGGGTAGAGGCATCTACTCTGGGATCCTTGGAAGCATTGCTAGAGTTTTTGAAAACGCCAGAAGTTAATATTCCTGTTAGTGGTATTAGCATCGGCCCTGTACACAAGAAGGATGTCATGAAGGCCAGTGTAATGCTTGAAAAGAGAAAGGAGTACGCCACTATCTTGGCATTTGATGTTAAAGTGACACCGGAGGCCCGTGACCTTGCAGATGAATTGGGTGTAAAGATTTTTCTTGCTGATATAATCGATCACTTATTTGACCAATTTAAGGCCTATATTGACAATCTCAAAGAGGAAAAGAAGGAGGAAGCAGCTGATGAAGCTGTTTTTCCATGTGTGCTCAAGATTTTTACCCAACTGTGTTTTCAACAAGAAGGATCCAATTGTCTTGGAGATTTTATTGATATTGGGTGCATTTCTTCCTTCGAGAATAACCACAAACCTGTAGATATAGTGAAGAAGGGGCTGAAGGTGGCTATCAAGATAGTCGGCACAGATTCTGATGAGCAGCAAAAGATGTATGGTAGGCATTTTGAAATTGAAGATGAACTTGTCAGCCATGTCTCCAGGAGGTCGATTGATGTACTTAAAACAAGCTACCGGGATGAACTGTCAATTGACGAGTGGAAGCTGGTTGCGAAATTGAAGAAACTGTTTGAGATACCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001882 GO:0001883 GO:0002181 GO:0002183 GO:0003674 GO:0003676 GO:0003723 GO:0003743 GO:0003824 GO:0003924 GO:0005488 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006412 GO:0006413 GO:0006518 GO:0006807 GO:0008135 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019001 GO:0019538 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032991 GO:0033290 GO:0034641 GO:0034645 GO:0035639 GO:0036094 GO:0043043 GO:0043167 GO:0043168 GO:0043170 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0070993 GO:0071704 GO:0097159 GO:0097367 GO:1901265 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko00195 ko01100 ko03013 map00195 map01100 map03013

Pfam Domains

Protein Families

GTP_EFTU GTP_EFTU_D2 IF-2

Protein Analysis

1225

Amino Acids

135.67

Weight (kDa)

5.32

Isoelectric Point (pI)

41.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GTP_EFTU	PF00009	670 - 863	1.6e-27	Elongation factor Tu GTP binding domain
IF-2	PF11987	980 - 1092	4.6e-20	Translation-initiation factor 2

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002582)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G21160 AT1G76720 AT1G76720 AT1G76810 AT2G27700 AT2G27700
fragaria_vesca	FvH4_2g13940 FvH4_2g13940 FvH4_2g13940 FvH4_4g36100 FvH4_4g36312
malus_domestica	MD05G1023200.v1.1 MD09G1087100.v1.1 MD10G1024100.v1.1
prunus_persica	Prupe.2G099300_v2.0.a1 Prupe.8G027900_v2.0.a1
pyrus_communis	pycom05g01470 pycom09g01240 pycom10g01600
rosa_chinensis	RchiOBHm_Chr4g0445721 RchiOBHm_Chr6g0275701
rosa_laevigata	RLG00000005714 RLG00000013337 RLG00000013444
rosa_multiflora	Rmu_co8469881.1_g000001 Rmu_sc0003637.1_g000011 Rmu_sc0003637.1_g000012 Rmu_sc0007122.1_g000003
rosa_roxburghii	Rroxscaffold_7G00193000
rosa_rugosa	Rorug04G0365200 Rorug06G0095700
rosa_samantha	Rh4AG426100 Rh4CG453100 Rh4DG434300 Rh6AG207600 Rh6BG210300 Rh6CG214100 Rh6DG203400
rosa_wichuraiana	Rw4G036490 Rw6G018100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2487
AasI	GACNNNNNNGTC	1 cut(s)	1093
Acc36I	ACCTGC	2 cut(s)	1448, 2387
AccB1I	GGYRCC	1 cut(s)	1267
AccB7I	CCANNNNNTGG	2 cut(s)	53, 1055
AccII	CGCG	1 cut(s)	224
AccIII	TCCGGA	1 cut(s)	1778
AclI	AACGTT	1 cut(s)	864
AclWI	GGATC	6 cut(s)	1432, 1445, 3021, 3034, 3378, 3391
AcsI	RAATTY	3 cut(s)	329, 2330, 2625
AcuI	CTGAAG	4 cut(s)	483, 1098, 2180, 3494
AfaI	GTAC	7 cut(s)	772, 2441, 2523, 2635, 3105, 3158, 3591
AfiI	CCNNNNNNNGG	8 cut(s)	50, 53, 175, 243, 1055, 1305, 3083, 3274
AflIII	ACRYGT	1 cut(s)	1276
AhdI	GACNNNNNGTC	1 cut(s)	2781
AhlI	ACTAGT	1 cut(s)	755
AjiI	CACGTC	1 cut(s)	2783
AjnI	CCWGG	4 cut(s)	84, 2167, 2191, 3572
AjuI	GAANNNNNNNTTGG	2 cut(s)	3354, 3386
AloI	GAACNNNNNNTCC	2 cut(s)	915, 947
Alw21I	GWGCWC	1 cut(s)	3348
Alw26I	GTCTC	3 cut(s)	971, 1079, 3574
AlwI	GGATC	6 cut(s)	1432, 1445, 3021, 3034, 3378, 3391
AlwNI	CAGNNNCTG	2 cut(s)	1958, 3506
Ama87I	CYCGRG	2 cut(s)	1730, 2763
Aor13HI	TCCGGA	1 cut(s)	1778
AoxI	GGCC	8 cut(s)	1296, 2583, 2662, 2912, 3097, 3126, 3198, 3276
ApaI	GGGCCC	1 cut(s)	2587
ApoI	RAATTY	3 cut(s)	329, 2330, 2625
Asp700I	GAANNNNTTC	2 cut(s)	824, 2550
AspLEI	GCGC	2 cut(s)	224, 704
AspS9I	GGNCC	8 cut(s)	1297, 2164, 2583, 2584, 2744, 2912, 3098, 3199
AsuC2I	CCSGG	1 cut(s)	3608
AsuHPI	GGTGA	8 cut(s)	100, 308, 425, 776, 1844, 2075, 2555, 3005
AvaI	CYCGRG	2 cut(s)	1730, 2763
AvaII	GGWCC	2 cut(s)	2164, 2744
BaeGI	GKGCMC	3 cut(s)	574, 2199, 2587
BaeI	ACNNNNGTAYC	2 cut(s)	726, 759
BamHI	GGATCC	3 cut(s)	1437, 3026, 3383
BanI	GGYRCC	1 cut(s)	1267
BanII	GRGCYC	1 cut(s)	2587
BauI	CACGAG	2 cut(s)	24, 2916
BbsI	GAAGAC	4 cut(s)	102, 729, 817, 945
Bbv12I	GWGCWC	1 cut(s)	3348
BbvCI	CCTCAGC	1 cut(s)	2025
BccI	CCATC	6 cut(s)	425, 572, 761, 1049, 1708, 1823
BcgI	CGANNNNNNTGC	2 cut(s)	470, 504
BciT130I	CCWGG	4 cut(s)	86, 2169, 2193, 3574
BcnI	CCSGG	1 cut(s)	3608
BcoDI	GTCTC	3 cut(s)	971, 1079, 3574
BcuI	ACTAGT	1 cut(s)	755
BfaI	CTAG	5 cut(s)	170, 756, 971, 1305, 3044
BfmI	CTRYAG	5 cut(s)	1101, 1170, 1950, 2731, 3450
BfuAI	ACCTGC	2 cut(s)	1448, 2387
BlpI	GCTNAGC	1 cut(s)	276
Bme1390I	CCNGG	5 cut(s)	86, 2169, 2193, 3574, 3608
Bme18I	GGWCC	2 cut(s)	2164, 2744
BmeRI	GACNNNNNGTC	1 cut(s)	2781
BmeT110I	CYCGRG	2 cut(s)	1730, 2763
BmgBI	CACGTC	1 cut(s)	2783
BmgT120I	GGNCC	8 cut(s)	1297, 2164, 2583, 2584, 2744, 2912, 3098, 3199
BmiI	GGNNCC	8 cut(s)	161, 1269, 1439, 2166, 2585, 2745, 3028, 3385
BmrFI	CCNGG	5 cut(s)	86, 2169, 2193, 3574, 3608
BmrI	ACTGGG	1 cut(s)	1043
BmuI	ACTGGG	1 cut(s)	1043
BpiI	GAAGAC	4 cut(s)	102, 729, 817, 945
BplI	GAGNNNNNCTC	2 cut(s)	2440, 2472
BpmI	CTGGAG	2 cut(s)	2091, 3556
Bpu10I	CCTNAGC	1 cut(s)	2025
Bpu1102I	GCTNAGC	1 cut(s)	276
BpuEI	CTTGAG	1 cut(s)	3332
BpuMI	CCSGG	1 cut(s)	3608
Bsa29I	ATCGAT	1 cut(s)	3252
BsaAI	YACGTR	1 cut(s)	2100
BsaJI	CCNNGG	8 cut(s)	129, 522, 2167, 2191, 2192, 2421, 2665, 3030
BsaWI	WCCGGW	2 cut(s)	1778, 3193
BsaXI	ACNNNNNCTCC	4 cut(s)	1484, 1514, 2991, 3021
Bsc4I	CCNNNNNNNGG	8 cut(s)	50, 53, 175, 243, 1055, 1305, 3083, 3274
Bse118I	RCCGGY	4 cut(s)	371, 761, 1588, 2888
Bse1I	ACTGG	5 cut(s)	237, 415, 1049, 1519, 3129
Bse3DI	GCAATG	5 cut(s)	707, 2256, 2412, 2626, 3038
BseAI	TCCGGA	1 cut(s)	1778
BseBI	CCWGG	4 cut(s)	86, 2169, 2193, 3574
BseCI	ATCGAT	1 cut(s)	3252
BseDI	CCNNGG	8 cut(s)	129, 522, 2167, 2191, 2192, 2421, 2665, 3030
BseLI	CCNNNNNNNGG	8 cut(s)	50, 53, 175, 243, 1055, 1305, 3083, 3274
BseMI	GCAATG	5 cut(s)	707, 2256, 2412, 2626, 3038
BseMII	CTCAG	7 cut(s)	111, 267, 1019, 1346, 1950, 2016, 2471
BseNI	ACTGG	5 cut(s)	237, 415, 1049, 1519, 3129
BseSI	GKGCMC	3 cut(s)	574, 2199, 2587
BseYI	CCCAGC	1 cut(s)	1860
BsgI	GTGCAG	2 cut(s)	1662, 2826
Bsh1236I	CGCG	1 cut(s)	224
BshFI	GGCC	8 cut(s)	1298, 2585, 2664, 2914, 3099, 3128, 3200, 3278
BshNI	GGYRCC	1 cut(s)	1267
BshVI	ATCGAT	1 cut(s)	3252
BsiHKAI	GWGCWC	1 cut(s)	3348
BsiHKCI	CYCGRG	2 cut(s)	1730, 2763
BsiSI	CCGG	8 cut(s)	320, 372, 762, 1589, 1779, 2889, 3194, 3607
BslFI	GGGAC	5 cut(s)	2150, 2683, 2757, 2769, 2883
BslI	CCNNNNNNNGG	8 cut(s)	50, 53, 175, 243, 1055, 1305, 3083, 3274
BsmAI	GTCTC	3 cut(s)	971, 1079, 3574
BsmBI	CGTCTC	1 cut(s)	971
BsmFI	GGGAC	5 cut(s)	2150, 2683, 2757, 2769, 2883
BsnI	GGCC	8 cut(s)	1298, 2585, 2664, 2914, 3099, 3128, 3200, 3278
BsoBI	CYCGRG	2 cut(s)	1730, 2763
Bsp120I	GGGCCC	1 cut(s)	2583
Bsp1286I	GDGCHC	4 cut(s)	574, 2199, 2587, 3348
Bsp13I	TCCGGA	1 cut(s)	1778
Bsp1407I	TGTACA	1 cut(s)	3103
Bsp143I	GATC	7 cut(s)	90, 466, 1437, 2716, 3026, 3253, 3383
Bsp1720I	GCTNAGC	1 cut(s)	276
BspANI	GGCC	8 cut(s)	1298, 2585, 2664, 2914, 3099, 3128, 3200, 3278
BspCNI	CTCAG	7 cut(s)	112, 268, 1018, 1347, 1951, 2017, 2470
BspDI	ATCGAT	1 cut(s)	3252
BspEI	TCCGGA	1 cut(s)	1778
BspFNI	CGCG	1 cut(s)	224
BspHI	TCATGA	5 cut(s)	469, 1708, 1996, 2707, 3120
BspLI	GGNNCC	8 cut(s)	161, 1269, 1439, 2166, 2585, 2745, 3028, 3385
BspMAI	CTGCAG	3 cut(s)	1105, 1954, 2735
BspMI	ACCTGC	2 cut(s)	1448, 2387
BspPI	GGATC	6 cut(s)	1432, 1445, 3021, 3034, 3378, 3391
BspQI	GCTCTTC	1 cut(s)	1737
BspT107I	GGYRCC	1 cut(s)	1267
BsrDI	GCAATG	5 cut(s)	707, 2256, 2412, 2626, 3038
BsrFI	RCCGGY	4 cut(s)	371, 761, 1588, 2888
BsrGI	TGTACA	1 cut(s)	3103
BsrI	ACTGG	5 cut(s)	237, 415, 1049, 1519, 3129
BssAI	RCCGGY	4 cut(s)	371, 761, 1588, 2888
BssECI	CCNNGG	8 cut(s)	129, 522, 2167, 2191, 2192, 2421, 2665, 3030
BssMI	GATC	7 cut(s)	90, 466, 1437, 2716, 3026, 3253, 3383
BssSI	CACGAG	2 cut(s)	24, 2916
BssT1I	CCWWGG	4 cut(s)	522, 2421, 2665, 3030
Bst2BI	CACGAG	2 cut(s)	24, 2916
Bst2UI	CCWGG	4 cut(s)	86, 2169, 2193, 3574
BstAPI	GCANNNNNTGC	1 cut(s)	1640
BstAUI	TGTACA	1 cut(s)	3103
BstBAI	YACGTR	1 cut(s)	2100
BstC8I	GCNNGC	8 cut(s)	489, 562, 996, 1215, 1901, 1950, 2738, 2890
BstENI	CCTNNNNNAGG	1 cut(s)	173
BstFNI	CGCG	1 cut(s)	224
BstHHI	GCGC	2 cut(s)	224, 704
BstKTI	GATC	7 cut(s)	93, 469, 1440, 2719, 3029, 3256, 3386
BstMAI	GTCTC	3 cut(s)	971, 1079, 3574
BstMBI	GATC	7 cut(s)	90, 466, 1437, 2716, 3026, 3253, 3383
BstNI	CCWGG	4 cut(s)	86, 2169, 2193, 3574
BstNSI	RCATGY	3 cut(s)	1280, 2740, 2882
BstSCI	CCNGG	5 cut(s)	84, 2167, 2191, 3572, 3606
BstSFI	CTRYAG	5 cut(s)	1101, 1170, 1950, 2731, 3450
BstSLI	GKGCMC	3 cut(s)	574, 2199, 2587
BstSNI	TACGTA	1 cut(s)	2100
BstUI	CGCG	1 cut(s)	224
BstV2I	GAAGAC	4 cut(s)	102, 729, 817, 945
BstX2I	RGATCY	3 cut(s)	1437, 3026, 3383
BstXI	CCANNNNNNTGG	1 cut(s)	3169
BstYI	RGATCY	3 cut(s)	1437, 3026, 3383
Bsu15I	ATCGAT	1 cut(s)	3252
BsuRI	GGCC	8 cut(s)	1298, 2585, 2664, 2914, 3099, 3128, 3200, 3278
BsuTUI	ATCGAT	1 cut(s)	3252
BtrI	CACGTC	1 cut(s)	2783
BtsI	GCAGTG	2 cut(s)	1551, 2892
BtsIMutI	CAGTG	6 cut(s)	408, 1551, 2585, 2636, 2892, 3136
BveI	ACCTGC	2 cut(s)	1448, 2387
Cac8I	GCNNGC	8 cut(s)	489, 562, 996, 1215, 1901, 1950, 2738, 2890
CaiI	CAGNNNCTG	2 cut(s)	1958, 3506
CciI	TCATGA	5 cut(s)	469, 1708, 1996, 2707, 3120
CfoI	GCGC	2 cut(s)	224, 704
Cfr10I	RCCGGY	4 cut(s)	371, 761, 1588, 2888
Cfr13I	GGNCC	8 cut(s)	1297, 2164, 2583, 2584, 2744, 2912, 3098, 3199
ClaI	ATCGAT	1 cut(s)	3252
CseI	GACGC	1 cut(s)	381
CsiI	ACCWGGT	1 cut(s)	84
Csp6I	GTAC	7 cut(s)	771, 2440, 2522, 2634, 3104, 3157, 3590
CviQI	GTAC	7 cut(s)	771, 2440, 2522, 2634, 3104, 3157, 3590
DpnI	GATC	7 cut(s)	92, 468, 1439, 2718, 3028, 3255, 3385
DpnII	GATC	7 cut(s)	90, 466, 1437, 2716, 3026, 3253, 3383
DraI	TTTAAA	1 cut(s)	1894
DrdI	GACNNNNNNGTC	1 cut(s)	1093
DriI	GACNNNNNGTC	1 cut(s)	2781
DseDI	GACNNNNNNGTC	1 cut(s)	1093
Eam1105I	GACNNNNNGTC	1 cut(s)	2781
EciI	GGCGGA	2 cut(s)	30, 155
Eco105I	TACGTA	1 cut(s)	2100
Eco130I	CCWWGG	4 cut(s)	522, 2421, 2665, 3030
Eco147I	AGGCCT	2 cut(s)	2664, 3278
Eco24I	GRGCYC	1 cut(s)	2587
Eco32I	GATATC	1 cut(s)	2935
Eco47I	GGWCC	2 cut(s)	2164, 2744
Eco57I	CTGAAG	4 cut(s)	483, 1098, 2180, 3494
Eco88I	CYCGRG	2 cut(s)	1730, 2763
EcoNI	CCTNNNNNAGG	1 cut(s)	173
EcoO109I	RGGNCCY	3 cut(s)	1297, 2164, 2744
EcoRI	GAATTC	1 cut(s)	2330
EcoRII	CCWGG	4 cut(s)	84, 2167, 2191, 3572
EcoRV	GATATC	1 cut(s)	2935
EcoT14I	CCWWGG	4 cut(s)	522, 2421, 2665, 3030
EcoT22I	ATGCAT	2 cut(s)	793, 2274
EcoT38I	GRGCYC	1 cut(s)	2587
ErhI	CCWWGG	4 cut(s)	522, 2421, 2665, 3030
Esp3I	CGTCTC	1 cut(s)	971
FalI	AAGNNNNNCTT	6 cut(s)	809, 841, 1284, 1316, 3540, 3572
FaqI	GGGAC	5 cut(s)	2150, 2683, 2757, 2769, 2883
FauI	CCCGC	2 cut(s)	43, 1892
FriOI	GRGCYC	1 cut(s)	2587
FspBI	CTAG	5 cut(s)	170, 756, 971, 1305, 3044
GlaI	GCGC	2 cut(s)	223, 703
GsaI	CCCAGC	1 cut(s)	1864
GsuI	CTGGAG	2 cut(s)	2091, 3556
HaeIII	GGCC	8 cut(s)	1298, 2585, 2664, 2914, 3099, 3128, 3200, 3278
HapII	CCGG	8 cut(s)	320, 372, 762, 1589, 1779, 2889, 3194, 3607
HgaI	GACGC	1 cut(s)	381
HhaI	GCGC	2 cut(s)	224, 704
Hin6I	GCGC	2 cut(s)	222, 702
HinP1I	GCGC	2 cut(s)	222, 702
HincII	GTYRAC	4 cut(s)	1623, 2038, 2263, 2775
HindII	GTYRAC	4 cut(s)	1623, 2038, 2263, 2775
HpaII	CCGG	8 cut(s)	320, 372, 762, 1589, 1779, 2889, 3194, 3607
HphI	GGTGA	8 cut(s)	100, 308, 425, 776, 1844, 2075, 2555, 3005
Hpy166II	GTNNAC	8 cut(s)	387, 1623, 2038, 2263, 2356, 2634, 2775, 3106
Hpy8I	GTNNAC	8 cut(s)	387, 1623, 2038, 2263, 2356, 2634, 2775, 3106
Hpy99I	CGWCG	3 cut(s)	104, 116, 397
HpyCH4IV	ACGT	3 cut(s)	864, 2099, 2782
HpySE526I	ACGT	3 cut(s)	864, 2099, 2782
HspAI	GCGC	2 cut(s)	222, 702
Kpn2I	TCCGGA	1 cut(s)	1778
KroI	GCCGGC	1 cut(s)	2888
KroNI	GCCGGC	1 cut(s)	2890
Kzo9I	GATC	7 cut(s)	90, 466, 1437, 2716, 3026, 3253, 3383
LguI	GCTCTTC	1 cut(s)	1737
LmnI	GCTCC	2 cut(s)	322, 1504
MabI	ACCWGGT	1 cut(s)	84
MaeI	CTAG	5 cut(s)	170, 756, 971, 1305, 3044
MaeII	ACGT	3 cut(s)	864, 2099, 2782
MalI	GATC	7 cut(s)	92, 468, 1439, 2718, 3028, 3255, 3385
MboI	GATC	7 cut(s)	90, 466, 1437, 2716, 3026, 3253, 3383
MfeI	CAATTG	5 cut(s)	492, 1218, 2676, 3388, 3621
MflI	RGATCY	3 cut(s)	1437, 3026, 3383
MhlI	GDGCHC	4 cut(s)	574, 2199, 2587, 3348
MlyI	GAGTC	4 cut(s)	145, 1103, 2306, 2918
Mph1103I	ATGCAT	2 cut(s)	793, 2274
MroI	TCCGGA	1 cut(s)	1778
MroNI	GCCGGC	1 cut(s)	2888
MroXI	GAANNNNTTC	2 cut(s)	824, 2550
MslI	CAYNNNNRTG	2 cut(s)	474, 1894
MspA1I	CMGCKG	5 cut(s)	50, 461, 1157, 1256, 3320
MspI	CCGG	8 cut(s)	320, 372, 762, 1589, 1779, 2889, 3194, 3607
MspR9I	CCNGG	5 cut(s)	86, 2169, 2193, 3574, 3608
MunI	CAATTG	5 cut(s)	492, 1218, 2676, 3388, 3621
MvaI	CCWGG	4 cut(s)	86, 2169, 2193, 3574
MvnI	CGCG	1 cut(s)	224
NaeI	GCCGGC	1 cut(s)	2890
NciI	CCSGG	1 cut(s)	3608
NdeII	GATC	7 cut(s)	90, 466, 1437, 2716, 3026, 3253, 3383
NgoMIV	GCCGGC	1 cut(s)	2888
NlaIV	GGNNCC	8 cut(s)	161, 1269, 1439, 2166, 2585, 2745, 3028, 3385
NmuCI	GTSAC	7 cut(s)	26, 413, 2044, 2220, 2298, 3187, 3203
NsiI	ATGCAT	2 cut(s)	793, 2274
NspI	RCATGY	3 cut(s)	1280, 2740, 2882
PaeI	GCATGC	1 cut(s)	2740
PaeR7I	CTCGAG	2 cut(s)	1730, 2763
PagI	TCATGA	5 cut(s)	469, 1708, 1996, 2707, 3120
PasI	CCCWGGG	1 cut(s)	2192
PceI	AGGCCT	2 cut(s)	2664, 3278
PciI	ACATGT	1 cut(s)	1276
PciSI	GCTCTTC	1 cut(s)	1737
PcsI	WCGNNNNNNNCGW	3 cut(s)	99, 108, 389
PdiI	GCCGGC	1 cut(s)	2890
PdmI	GAANNNNTTC	2 cut(s)	824, 2550
PflFI	GACNNNGTC	1 cut(s)	2042
PflMI	CCANNNNNTGG	2 cut(s)	53, 1055
PfoI	TCCNGGA	1 cut(s)	3572
PleI	GAGTC	4 cut(s)	144, 1102, 2305, 2918
PpsI	GAGTC	4 cut(s)	144, 1102, 2305, 2918
Ppu21I	YACGTR	1 cut(s)	2100
PpuMI	RGGWCCY	2 cut(s)	2164, 2744
PscI	ACATGT	1 cut(s)	1276
PsiI	TTATAA	1 cut(s)	2487
Psp1406I	AACGTT	1 cut(s)	864
Psp5II	RGGWCCY	2 cut(s)	2164, 2744
Psp6I	CCWGG	4 cut(s)	84, 2167, 2191, 3572
PspFI	CCCAGC	1 cut(s)	1860
PspGI	CCWGG	4 cut(s)	84, 2167, 2191, 3572
PspN4I	GGNNCC	8 cut(s)	161, 1269, 1439, 2166, 2585, 2745, 3028, 3385
PspOMI	GGGCCC	1 cut(s)	2583
PspPI	GGNCC	8 cut(s)	1297, 2164, 2583, 2584, 2744, 2912, 3098, 3199
PspPPI	RGGWCCY	2 cut(s)	2164, 2744
PspXI	VCTCGAGB	1 cut(s)	2763
PstI	CTGCAG	3 cut(s)	1105, 1954, 2735
PstNI	CAGNNNCTG	2 cut(s)	1958, 3506
PsuI	RGATCY	3 cut(s)	1437, 3026, 3383
PsyI	GACNNNGTC	1 cut(s)	2042
PvuII	CAGCTG	3 cut(s)	461, 1256, 3320
RsaI	GTAC	7 cut(s)	772, 2441, 2523, 2635, 3105, 3158, 3591
RsaNI	GTAC	7 cut(s)	771, 2440, 2522, 2634, 3104, 3157, 3590
RseI	CAYNNNNRTG	2 cut(s)	474, 1894
SapI	GCTCTTC	1 cut(s)	1737
Sau3AI	GATC	7 cut(s)	90, 466, 1437, 2716, 3026, 3253, 3383
Sau96I	GGNCC	8 cut(s)	1297, 2164, 2583, 2584, 2744, 2912, 3098, 3199
SchI	GAGTC	4 cut(s)	145, 1103, 2306, 2918
ScrFI	CCNGG	5 cut(s)	86, 2169, 2193, 3574, 3608
SduI	GDGCHC	4 cut(s)	574, 2199, 2587, 3348
SexAI	ACCWGGT	1 cut(s)	84
SfcI	CTRYAG	5 cut(s)	1101, 1170, 1950, 2731, 3450
Sfr274I	CTCGAG	2 cut(s)	1730, 2763
SinI	GGWCC	2 cut(s)	2164, 2744
SlaI	CTCGAG	2 cut(s)	1730, 2763
SmiI	ATTTAAAT	1 cut(s)	1894
SmiMI	CAYNNNNRTG	2 cut(s)	474, 1894
SmlI	CTYRAG	3 cut(s)	1730, 2763, 3347
SmoI	CTYRAG	3 cut(s)	1730, 2763, 3347
SnaBI	TACGTA	1 cut(s)	2100
SpeI	ACTAGT	1 cut(s)	755
SphI	GCATGC	1 cut(s)	2740
SseBI	AGGCCT	2 cut(s)	2664, 3278
SspI	AATATT	1 cut(s)	3073
SspMI	CTAG	5 cut(s)	170, 756, 971, 1305, 3044
StuI	AGGCCT	2 cut(s)	2664, 3278
StyD4I	CCNGG	5 cut(s)	84, 2167, 2191, 3572, 3606
StyI	CCWWGG	4 cut(s)	522, 2421, 2665, 3030
SwaI	ATTTAAAT	1 cut(s)	1894
TaiI	ACGT	3 cut(s)	867, 2102, 2785
TaqI	TCGA	7 cut(s)	93, 200, 1731, 2764, 3252, 3432, 3581
TatI	WGTACW	3 cut(s)	2439, 3103, 3589
TauI	GCSGC	3 cut(s)	1154, 1160, 2385
TscAI	CASTG	6 cut(s)	415, 1551, 2585, 2636, 2899, 3136
TseFI	GTSAC	7 cut(s)	26, 413, 2044, 2220, 2298, 3187, 3203
Tsp45I	GTSAC	7 cut(s)	26, 413, 2044, 2220, 2298, 3187, 3203
TspGWI	ACGGA	3 cut(s)	100, 2101, 2111
TspRI	CASTG	6 cut(s)	415, 1551, 2585, 2636, 2899, 3136
Tth111I	GACNNNGTC	1 cut(s)	2042
Van91I	CCANNNNNTGG	2 cut(s)	53, 1055
VpaK11BI	GGWCC	2 cut(s)	2164, 2744
XagI	CCTNNNNNAGG	1 cut(s)	173
XapI	RAATTY	3 cut(s)	329, 2330, 2625
XceI	RCATGY	3 cut(s)	1280, 2740, 2882
XhoI	CTCGAG	2 cut(s)	1730, 2763
XmnI	GAANNNNTTC	2 cut(s)	824, 2550
XspI	CTAG	5 cut(s)	170, 756, 971, 1305, 3044
Zsp2I	ATGCAT	2 cut(s)	793, 2274

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.