Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0002582

Physical Location & Seq

Genomic Coordinates Forward (+)

12238780 .. 12246567

Gene Structure 7788 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g13940.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4149 bp

ATGGGTCGGAAGAAGCCGACTGCTCGCGGCGGTGACGAGGAGAATCCGCAACCGGCGGGTGGTGGGAAATCGAAGAAGAAGAACCTGGTGATCGAAGACGACGAGTATTCCGTCGGAACTGAGCTGTCCGAGGAGAGTCAGGTCCAGGAAGACAAGGTTGTTGCTCCTCCTAGTAAAGGGAAGAAGGCCAAAAAGGGGGGTTCGAAAGCTGCCAAGGGAGTGGATGAGGAGTTTGAGGGGGCCGGAGAGGATGAAGATGAGGTTCCGCAAGTTGTGTTCACTGGGAAGAAGAAGGGGAAGTCGAAGAAGAGTGGTGGGAATACGTTCAGCTCGTCGGGTTTCGGGTTGCTTGAGGATGAGGATAAGGATAAGGATGGGGAGGCTGATGAGCAGTCTGCGGTAACAGGTGATGAAGAGGAGGAGGAGGATGCTCCGGCGTTGAAATTTTCGGGGAAGAAGAAACCTTCAAAGTGGTCGAAGAAAACTTTGTTTGCGGGGTCGGCTTTTGATGCCATTGGTGATGAAGGGGATAATGATGGTGAGGTGGATGATGATATTGTGGATAAGAGTAATGCAGCTGAAGATGATGAGAAAGACGACGATCCTGTGATTGCTTTTACGGGGAAGAAGAAGGCTTCCAAGGGTGGAAAGAAGGGTGGGAGTGTGTTTGCAGCGGCCAGCTTTAGTGCTCCTGATGATGAGGAGGAGGAGGATGAAAAGAATGATGAGGATGACGATATTCCTCAGATTACTTTCTCTGGTAAGAAAAAAAAGTCTTCAAAGACTTCAAAGAAGAGTGGTGGTAATTCTTTCAGTGCAGCATTGCTTGACGATGAAGATGATGAAGATACTTCCATTTCGAAACCAACTACAGCTGGTGATGATACAGTTGAGGATGAAGGTGCATCTATGATTACATTCACTGGTAAAAAGAAGTCTTCAAAGAAGAAAAGTAATAGTGTGTTTACTGCAGTCGGTGAAGATGCTGGGGTTGGGAGTGAAATTGCAGATGTAGTTGAGCCCGAACAACCAGCTACCGCTACTAGTAAAATTGAAGCTGATTATTCTAACAACAATAAGAACCAAGATCCAGAACCTTCAAAAAGTAAGAAAAAGAAGAAGAAGAATGTAAGGACTGCTCAAGAAGAGGATGATTTGGATAAGATTCTTGCAGAGCTAGGTGAGGTTCCATCAAAACCTGCTGCTCCTCAGAAAGAGGAGAAAGTTGAGGTTCAGCCTGCCGATTCTCAAAAAGAGGAGAAAGTTGAGGTTCAACCTGATGCTGTTGCCCCAGTTGGTGGGTCTGCTGCAAAGGAAGCTGAAGTAGAGACTGTTGAGTCTGCTGCAGCAAAGAAGAAGAAAAAGAAGAAGGAGAAGGAAAAGGAGAAAAAGGCGGCAGCAGCAGCATCTGTAGATGTCAAGGATGAAAAACAAGAACAAGTGAAAATTGAGCTAGCAATTGAACCAAAGAAGAAGGAAGTGAAAGGCAAAGCAGCTGAAAAGAAGGTGCCTAAACATGTCAGGGAGATGCAAGAGGCCCTTGCTAGGCGACAGGAAGAAGAAGAGAGGAAGAAAAAGGAAGAAGAAGAGAAACTGAAAAAAGAAGAAGAGGAGAGGCTTAAGCAAGAAGAACTTGAGAGGCAAAAGGAGGAGGCAAAGCGGAAGAAAAAAGAGAGGGAAAAGGAGAGGATCCAAGAGTTGAAGAAGGCAGGTTTATATAAGACTCCCAAGCAAAAAGAAGAGGACAGGCGACTACAGCAAATGAGAGACCAGTTTCTTAAAAATAATCCCAATGCAGTGGAGGGTGTACCTCCTACCACAGAAAACGAAAAACCGGCTAAAAGACCTTTGTATCAGAAAAAGAAGTCAAAACCAGCACCAAATCAAGCAAATGGTGCAGCTACTATGAAGCCTGTGGAACTCACAGAAGAAAGAAAAGAAGAAAACCAGCAAGTTAATGTTCCTGAGCTTCACTCTGTGGAACTTGAGAAGGTTGAGGAGCAGGAATCAGTTGAAGTTGCAGAGAAGCTATCCGAAGTTTCTGAATCTGTTAATGAGAATGGAGTGGAACAAGAAGAGGATGATGATGATGAATGGGATGCAAAAAGCTGGGATGATGATGCTGTTGTTAACCTTTCATTCAAAAGCGGGTTTTCTGATGAAGAGGTCGAACCTGTTGTGAATAAAGGGATGAAGCCTGCAGTTTCTGTGCAGAAAACCATTCCTTCTCAACCAATAAAGCCTCATGAGGTTGAGAATCAGAAAAAACAATCTGAGGTGGAAGTTGACAAAGGTAAGAAGAAAGATGCTGCTGCAAAGAAAAAAGCACCAAATTCAGATTCTGCCTCTAAAGAGGGTGAAGATAATCTTCGCTCTCCCATTTGCTGCATTATGGGCCATGTTGATACTGGTAAAACTAAGCTTCTGGATTGTATTCGAGGCACAAATGTTCAGGAAGGTGAGGCCGGAGGTATTACTCAACAGATTGGTGCGACATATTTTCCTGCAGAAAACATCCGTGAGAGGACTAAGGAACTGAAAGCTGATGCAAAGCTGAAGGTCCCAGGTCTATTGGTCATTGATACCCCTGGGCACGAGTCATTTACAAATTTAAGGTCGCGGGGTTCAGGTTTATGCGATATTGCAATTTTGGTTGTTGACATTATGCATGGCTTAGAGCCTCAAACAATAGAGTCACTCAATCTCTTGAAAATGAGGAATACCGAATTCATTGTTGCGTTGAATAAGGTGGACAGGCTCTATGGATGGAAAACCTGCCGCAACCAGCCAATAGGGAAGGCAATGAAGCAACAATCCAAGGATGTTGTAAATGAGTACAATATGAGGGTTTCTCAGATTATCACTCAATTCATGGAGCAAGGCTTAAATACTGAGTTGTACTATAAAAATAAAGAAATGGGAGAAACATACAGTATTGTACCTACAAGTGCCATCAGTGGTGAAGGGATTCCAGATTTGTTATTACTATTGGTTCAGTGGGCCCAGAAAACCATGGTTGAAAAACTTACATACAGCAATGAAATTCAGTGTACGGTATTGGAGGTCAAGGTTATTGAAGGCCTTGGGACAACAATTGATGTTGTGTTGGTTAATGGTGTGCTTCATGAAGGAGATCAAATAGTGGTTTGCGGCATGCAGGGACCTATTGTTACTTCAATTCGAGCTTTGTTGACACCACATCCCATGAAAGAACTTCGTGTCAAGGGAACTTATCAGCACCATAGTCAAATCAAAGCTGCACAAGGTATCAAGATCACAGCACAGGGACTTGAACATGCTATTGCTGGCACTGCTCTATATGTTGTTGGGCCTGGTGACGACTTGGATGATATCAAGGAAACAGCTATGGAAGATATGAAATCAGTCTTGAATAGGATTGACAAGAGTGGTGAGGGAGTTTGTGTTCAAGCATCTACTCTAGGATCCTTGGAAGCATTGCTGGAGTTTCTGAAAACTCCAGAAGTTAATATTCCTGTTAGTGGTATTAGCATTGGCCCTGTACACAAGAAGGATGTCATGAAGGCCAGTGTAATGCTTGAAAAGAAAAAGGAGTATGCCACTATCTTGGCATTTGATGTTAAAGTGACACCTGAGGCCCGGGACCTTGCAGAAGAATTGGGTGTGAAGATTTTTATTGCTGACATAATCTATCACTTATTTGACCAATTTAAGGCCTATATCGACAATCTCAAAGAGGAAAAGAAGAAGGAAGCAGCTGATGAAGCTGTTTTTCCGTGTGTGCTCAAGATTTTACCCAACTGTGTTTTCAACAAAAAGGATCCAATTGTCTTGGGTGTTGATGTTCTTGAAGGCATTGCAAAGGTAGGGACTCCAATTTGCATTCCTCAGAGAGATTTTATTGACATTGGGCGTATTGCATCCATAGAGAATAATCACAAACCTGTAGATGTTGCGAAAAAGGGGCTGAAGGTGGCTATCAAGATCGTTGGCACAAATTCTGATGAACAGCAAAAGATGTATGGAAGGCATTTTGAAATTGAAGATGAACTTGTCAGCCATATCTCCAGGAGGTCGATTGATGTACTTAAGACAAGCTACAGGGATGAGCTGTCAATTGATGAGTGGAAGCTGGTTGTGAAATTGAAGAAACTTTTTGAGATACCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001882 GO:0001883 GO:0002181 GO:0002183 GO:0003674 GO:0003676 GO:0003723 GO:0003743 GO:0003824 GO:0003924 GO:0005488 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006412 GO:0006413 GO:0006518 GO:0006807 GO:0008135 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019001 GO:0019538 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032991 GO:0033290 GO:0034641 GO:0034645 GO:0035639 GO:0036094 GO:0043043 GO:0043167 GO:0043168 GO:0043170 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0070993 GO:0071704 GO:0097159 GO:0097367 GO:1901265 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko00195 ko01100 ko03013 map00195 map01100 map03013

Pfam Domains

Protein Families

GTP_EFTU GTP_EFTU_D2 IF-2

Protein Analysis

1383

Amino Acids

152.31

Weight (kDa)

5.2

Isoelectric Point (pI)

37.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GTP_EFTU	PF00009	793 - 1002	9.2e-35	Elongation factor Tu GTP binding domain
IF-2	PF11987	1119 - 1231	4.6e-23	Translation-initiation factor 2
GTP_EFTU_D4	PF14578	1252 - 1325	1.2e-14	Elongation factor Tu domain 4

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002582)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G21160 AT1G76720 AT1G76720 AT1G76810 AT2G27700 AT2G27700
fragaria_vesca	FvH4_2g13940 FvH4_2g13940 FvH4_2g13940 FvH4_4g36100 FvH4_4g36312
malus_domestica	MD05G1023200.v1.1 MD09G1087100.v1.1 MD10G1024100.v1.1
prunus_persica	Prupe.2G099300_v2.0.a1 Prupe.8G027900_v2.0.a1
pyrus_communis	pycom05g01470 pycom09g01240 pycom10g01600
rosa_chinensis	RchiOBHm_Chr4g0445721 RchiOBHm_Chr6g0275701
rosa_laevigata	RLG00000005714 RLG00000013337 RLG00000013444
rosa_multiflora	Rmu_co8469881.1_g000001 Rmu_sc0003637.1_g000011 Rmu_sc0003637.1_g000012 Rmu_sc0007122.1_g000003
rosa_roxburghii	Rroxscaffold_7G00193000
rosa_rugosa	Rorug04G0365200 Rorug06G0095700
rosa_samantha	Rh4AG426100 Rh4CG453100 Rh4DG434300 Rh6AG207600 Rh6BG210300 Rh6CG214100 Rh6DG203400
rosa_wichuraiana	Rw4G036490 Rw6G018100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1336
Acc36I	ACCTGC	3 cut(s)	1207, 1700, 2783
AccB1I	GGYRCC	1 cut(s)	1507
AccB7I	CCANNNNNTGG	1 cut(s)	1298
AccII	CGCG	2 cut(s)	27, 2620
AclWI	GGATC	8 cut(s)	596, 1082, 1684, 1697, 3438, 3451, 3794, 3807
AcoI	YGGCCR	1 cut(s)	675
AcsI	RAATTY	6 cut(s)	443, 2332, 2608, 2726, 3042, 3976
AcuI	CTGAAG	4 cut(s)	600, 1341, 2576, 3968
AdeI	CACNNNGTG	1 cut(s)	1978
AfaI	GTAC	7 cut(s)	1809, 2837, 2900, 2940, 3052, 3522, 4065
AfiI	CCNNNNNNNGG	7 cut(s)	59, 176, 195, 1298, 1545, 3500, 3691
AflII	CTTAAG	2 cut(s)	1619, 4067
AflIII	ACRYGT	1 cut(s)	1516
AhlI	ACTAGT	1 cut(s)	1043
AjnI	CCWGG	6 cut(s)	84, 144, 2563, 2587, 3331, 4046
AjuI	GAANNNNNNNTTGG	4 cut(s)	2323, 2355, 3770, 3802
AloI	GAACNNNNNNTCC	2 cut(s)	3408, 3440
Alw21I	GWGCWC	2 cut(s)	691, 3765
Alw26I	GTCTC	2 cut(s)	1322, 1761
AlwI	GGATC	8 cut(s)	596, 1082, 1684, 1697, 3438, 3451, 3794, 3807
AlwNI	CAGNNNCTG	3 cut(s)	1409, 2207, 2342
Ama87I	CYCGRG	1 cut(s)	3618
ApaI	GGGCCC	1 cut(s)	3004
ApoI	RAATTY	6 cut(s)	443, 2332, 2608, 2726, 3042, 3976
ArsI	GACNNNNNNTTYG	2 cut(s)	2600, 2632
Asp700I	GAANNNNTTC	2 cut(s)	323, 2967
AsuC2I	CCSGG	2 cut(s)	3619, 3620
AsuII	TTCGAA	2 cut(s)	203, 860
AsuNHI	GCTAGC	1 cut(s)	1453
AvaI	CYCGRG	1 cut(s)	3618
AvaII	GGWCC	4 cut(s)	142, 2560, 3161, 3622
AxyI	CCTNAGG	1 cut(s)	3612
BaeGI	GKGCMC	2 cut(s)	2595, 3004
BaeI	ACNNNNGTAYC	2 cut(s)	1835, 1868
BamHI	GGATCC	3 cut(s)	1689, 3443, 3799
BanI	GGYRCC	1 cut(s)	1507
BanII	GRGCYC	2 cut(s)	1023, 3004
BauI	CACGAG	1 cut(s)	2594
BbsI	GAAGAC	4 cut(s)	102, 156, 768, 930
Bbv12I	GWGCWC	2 cut(s)	691, 3765
BccI	CCATC	5 cut(s)	368, 530, 1198, 2760, 2960
BciT130I	CCWGG	6 cut(s)	86, 146, 2565, 2589, 3333, 4048
BcnI	CCSGG	2 cut(s)	3619, 3620
BcoDI	GTCTC	2 cut(s)	1322, 1761
BcuI	ACTAGT	1 cut(s)	1043
BfaI	CTAG	6 cut(s)	171, 1044, 1178, 1454, 1545, 3440
BfmI	CTRYAG	9 cut(s)	870, 969, 1344, 1410, 1754, 2199, 2505, 3924, 4078
BfrI	CTTAAG	2 cut(s)	1619, 4067
BfuAI	ACCTGC	3 cut(s)	1207, 1700, 2783
Bme1390I	CCNGG	8 cut(s)	86, 146, 2565, 2589, 3333, 3619, 3620, 4048
Bme18I	GGWCC	4 cut(s)	142, 2560, 3161, 3622
BmeT110I	CYCGRG	1 cut(s)	3618
BmrFI	CCNGG	8 cut(s)	86, 146, 2565, 2589, 3333, 3619, 3620, 4048
BmrI	ACTGGG	2 cut(s)	291, 1286
BmtI	GCTAGC	1 cut(s)	1457
BmuI	ACTGGG	2 cut(s)	291, 1286
BpiI	GAAGAC	4 cut(s)	102, 156, 768, 930
BplI	GAGNNNNNCTC	4 cut(s)	1958, 1990, 2836, 2868
BpmI	CTGGAG	3 cut(s)	3462, 3482, 4030
Bpu10I	CCTNAGC	1 cut(s)	1965
Bpu14I	TTCGAA	2 cut(s)	203, 860
BpuEI	CTTGAG	5 cut(s)	371, 1125, 1655, 2006, 3749
BpuMI	CCSGG	2 cut(s)	3619, 3620
BsaI	GGTCTC	1 cut(s)	1761
BsaXI	ACNNNNNCTCC	2 cut(s)	3408, 3438
Bsc4I	CCNNNNNNNGG	7 cut(s)	59, 176, 195, 1298, 1545, 3500, 3691
Bse118I	RCCGGY	2 cut(s)	52, 1834
Bse1I	ACTGG	6 cut(s)	286, 928, 1292, 1771, 2413, 3546
Bse21I	CCTNAGG	1 cut(s)	3612
Bse3DI	GCAATG	5 cut(s)	821, 2808, 3043, 3455, 3834
BseBI	CCWGG	6 cut(s)	86, 146, 2565, 2589, 3333, 4048
BseLI	CCNNNNNNNGG	7 cut(s)	59, 176, 195, 1298, 1545, 3500, 3691
BseMI	GCAATG	5 cut(s)	821, 2808, 3043, 3455, 3834
BseMII	CTCAG	9 cut(s)	111, 758, 1223, 1956, 2265, 2867, 2883, 3603, 3881
BseNI	ACTGG	6 cut(s)	286, 928, 1292, 1771, 2413, 3546
BseSI	GKGCMC	2 cut(s)	2595, 3004
BseYI	CCCAGC	2 cut(s)	986, 2109
BsgI	GTGCAG	4 cut(s)	837, 1917, 2231, 3243
Bsh1236I	CGCG	2 cut(s)	27, 2620
BshNI	GGYRCC	1 cut(s)	1507
BsiHKAI	GWGCWC	2 cut(s)	691, 3765
BsiHKCI	CYCGRG	1 cut(s)	3618
BsiSI	CCGG	6 cut(s)	53, 243, 434, 1835, 2466, 3619
BslFI	GGGAC	6 cut(s)	2546, 3100, 3174, 3300, 3635, 3862
BslI	CCNNNNNNNGG	7 cut(s)	59, 176, 195, 1298, 1545, 3500, 3691
BsmAI	GTCTC	2 cut(s)	1322, 1761
BsmFI	GGGAC	6 cut(s)	2546, 3100, 3174, 3300, 3635, 3862
BsmI	GAATGC	1 cut(s)	3861
Bso31I	GGTCTC	1 cut(s)	1761
BsoBI	CYCGRG	1 cut(s)	3618
Bsp119I	TTCGAA	2 cut(s)	203, 860
Bsp120I	GGGCCC	1 cut(s)	3000
Bsp1286I	GDGCHC	5 cut(s)	691, 1023, 2595, 3004, 3765
Bsp1407I	TGTACA	1 cut(s)	3520
Bsp143I	GATC	9 cut(s)	90, 601, 1087, 1689, 3133, 3273, 3443, 3799, 3963
Bsp19I	CCATGG	1 cut(s)	3012
BspCNI	CTCAG	9 cut(s)	112, 757, 1222, 1957, 2266, 2866, 2884, 3604, 3880
BspFNI	CGCG	2 cut(s)	27, 2620
BspHI	TCATGA	3 cut(s)	2245, 3124, 3537
BspMAI	CTGCAG	4 cut(s)	973, 1348, 2203, 2509
BspMI	ACCTGC	3 cut(s)	1207, 1700, 2783
BspOI	GCTAGC	1 cut(s)	1457
BspPI	GGATC	8 cut(s)	596, 1082, 1684, 1697, 3438, 3451, 3794, 3807
BspT104I	TTCGAA	2 cut(s)	203, 860
BspT107I	GGYRCC	1 cut(s)	1507
BspTI	CTTAAG	2 cut(s)	1619, 4067
BspTNI	GGTCTC	1 cut(s)	1761
BsrDI	GCAATG	5 cut(s)	821, 2808, 3043, 3455, 3834
BsrFI	RCCGGY	2 cut(s)	52, 1834
BsrGI	TGTACA	1 cut(s)	3520
BsrI	ACTGG	6 cut(s)	286, 928, 1292, 1771, 2413, 3546
BssAI	RCCGGY	2 cut(s)	52, 1834
BssMI	GATC	9 cut(s)	90, 601, 1087, 1689, 3133, 3273, 3443, 3799, 3963
BssSI	CACGAG	1 cut(s)	2594
BssT1I	CCWWGG	6 cut(s)	213, 639, 2817, 3012, 3082, 3447
Bst2BI	CACGAG	1 cut(s)	2594
Bst2UI	CCWGG	6 cut(s)	86, 146, 2565, 2589, 3333, 4048
Bst4CI	ACNGT	5 cut(s)	889, 1333, 2933, 3055, 3782
BstAFI	CTTAAG	2 cut(s)	1619, 4067
BstAPI	GCANNNNNTGC	1 cut(s)	1895
BstAUI	TGTACA	1 cut(s)	3520
BstBI	TTCGAA	2 cut(s)	203, 860
BstC8I	GCNNGC	7 cut(s)	25, 679, 1239, 1455, 2199, 3155, 3307
BstDSI	CCRYGG	1 cut(s)	3012
BstENI	CCTNNNNNAGG	1 cut(s)	174
BstFNI	CGCG	2 cut(s)	27, 2620
BstKTI	GATC	9 cut(s)	93, 604, 1090, 1692, 3136, 3276, 3446, 3802, 3966
BstMAI	GTCTC	2 cut(s)	1322, 1761
BstMBI	GATC	9 cut(s)	90, 601, 1087, 1689, 3133, 3273, 3443, 3799, 3963
BstNI	CCWGG	6 cut(s)	86, 146, 2565, 2589, 3333, 4048
BstNSI	RCATGY	3 cut(s)	1520, 3157, 3299
BstSCI	CCNGG	8 cut(s)	84, 144, 2563, 2587, 3331, 3617, 3618, 4046
BstSFI	CTRYAG	9 cut(s)	870, 969, 1344, 1410, 1754, 2199, 2505, 3924, 4078
BstSLI	GKGCMC	2 cut(s)	2595, 3004
BstUI	CGCG	2 cut(s)	27, 2620
BstV2I	GAAGAC	4 cut(s)	102, 156, 768, 930
BstX2I	RGATCY	4 cut(s)	1087, 1689, 3443, 3799
BstXI	CCANNNNNNTGG	3 cut(s)	220, 1798, 3586
BstYI	RGATCY	4 cut(s)	1087, 1689, 3443, 3799
Bsu36I	CCTNAGG	1 cut(s)	3612
BtgI	CCRYGG	1 cut(s)	3012
BtsI	GCAGTG	2 cut(s)	1803, 3309
BtsIMutI	CAGTG	9 cut(s)	279, 820, 921, 1803, 2962, 3002, 3053, 3309, 3553
BveI	ACCTGC	3 cut(s)	1207, 1700, 2783
Cac8I	GCNNGC	7 cut(s)	25, 679, 1239, 1455, 2199, 3155, 3307
CaiI	CAGNNNCTG	3 cut(s)	1409, 2207, 2342
CciI	TCATGA	3 cut(s)	2245, 3124, 3537
Cfr10I	RCCGGY	2 cut(s)	52, 1834
Cfr9I	CCCGGG	1 cut(s)	3618
CsiI	ACCWGGT	1 cut(s)	84
Csp6I	GTAC	7 cut(s)	1808, 2836, 2899, 2939, 3051, 3521, 4064
CviQI	GTAC	7 cut(s)	1808, 2836, 2899, 2939, 3051, 3521, 4064
DpnI	GATC	9 cut(s)	92, 603, 1089, 1691, 3135, 3275, 3445, 3801, 3965
DpnII	GATC	9 cut(s)	90, 601, 1087, 1689, 3133, 3273, 3443, 3799, 3963
DraIII	CACNNNGTG	1 cut(s)	1978
DrdI	GACNNNNNNGTC	1 cut(s)	1336
DseDI	GACNNNNNNGTC	1 cut(s)	1336
EaeI	YGGCCR	1 cut(s)	675
Eco130I	CCWWGG	6 cut(s)	213, 639, 2817, 3012, 3082, 3447
Eco147I	AGGCCT	2 cut(s)	3081, 3695
Eco24I	GRGCYC	2 cut(s)	1023, 3004
Eco31I	GGTCTC	1 cut(s)	1761
Eco32I	GATATC	1 cut(s)	3352
Eco47I	GGWCC	4 cut(s)	142, 2560, 3161, 3622
Eco57I	CTGAAG	4 cut(s)	600, 1341, 2576, 3968
Eco81I	CCTNAGG	1 cut(s)	3612
Eco88I	CYCGRG	1 cut(s)	3618
EcoNI	CCTNNNNNAGG	1 cut(s)	174
EcoO109I	RGGNCCY	4 cut(s)	1537, 2560, 3161, 3622
EcoRI	GAATTC	1 cut(s)	2726
EcoRII	CCWGG	6 cut(s)	84, 144, 2563, 2587, 3331, 4046
EcoRV	GATATC	1 cut(s)	3352
EcoT14I	CCWWGG	6 cut(s)	213, 639, 2817, 3012, 3082, 3447
EcoT22I	ATGCAT	1 cut(s)	2670
EcoT38I	GRGCYC	2 cut(s)	1023, 3004
ErhI	CCWWGG	6 cut(s)	213, 639, 2817, 3012, 3082, 3447
FalI	AAGNNNNNCTT	8 cut(s)	1524, 1556, 1617, 1649, 2352, 2384, 4014, 4046
FaqI	GGGAC	6 cut(s)	2546, 3100, 3174, 3300, 3635, 3862
FauI	CCCGC	4 cut(s)	49, 487, 2141, 2613
FriOI	GRGCYC	2 cut(s)	1023, 3004
FspBI	CTAG	6 cut(s)	171, 1044, 1178, 1454, 1545, 3440
GsaI	CCCAGC	2 cut(s)	990, 2113
GsuI	CTGGAG	3 cut(s)	3462, 3482, 4030
HapII	CCGG	6 cut(s)	53, 243, 434, 1835, 2466, 3619
HincII	GTYRAC	4 cut(s)	2131, 2287, 2659, 3192
HindII	GTYRAC	4 cut(s)	2131, 2287, 2659, 3192
HindIII	AAGCTT	1 cut(s)	2420
HpaI	GTTAAC	1 cut(s)	2131
HpaII	CCGG	6 cut(s)	53, 243, 434, 1835, 2466, 3619
Hpy99I	CGWCG	4 cut(s)	104, 116, 337, 602
HpyCH4III	ACNGT	5 cut(s)	889, 1333, 2933, 3055, 3782
HpyCH4IV	ACGT	1 cut(s)	323
HpySE526I	ACGT	1 cut(s)	323
KspAI	GTTAAC	1 cut(s)	2131
Kzo9I	GATC	9 cut(s)	90, 601, 1087, 1689, 3133, 3273, 3443, 3799, 3963
LmnI	GCTCC	6 cut(s)	169, 436, 694, 1210, 1999, 2875
MabI	ACCWGGT	1 cut(s)	84
MaeI	CTAG	6 cut(s)	171, 1044, 1178, 1454, 1545, 3440
MaeII	ACGT	1 cut(s)	323
MaeIII	GTNAC	6 cut(s)	32, 400, 2694, 3169, 3335, 3604
MalI	GATC	9 cut(s)	92, 603, 1089, 1691, 3135, 3275, 3445, 3801, 3965
MboI	GATC	9 cut(s)	90, 601, 1087, 1689, 3133, 3273, 3443, 3799, 3963
MfeI	CAATTG	4 cut(s)	1458, 3093, 3804, 4095
MflI	RGATCY	4 cut(s)	1087, 1689, 3443, 3799
MhlI	GDGCHC	5 cut(s)	691, 1023, 2595, 3004, 3765
MlyI	GAGTC	6 cut(s)	145, 1346, 1717, 2606, 2702, 3844
MmeI	TCCRAC	1 cut(s)	94
Mph1103I	ATGCAT	1 cut(s)	2670
MroXI	GAANNNNTTC	2 cut(s)	323, 2967
MspA1I	CMGCKG	5 cut(s)	578, 674, 875, 1496, 3737
MspCI	CTTAAG	2 cut(s)	1619, 4067
MspI	CCGG	6 cut(s)	53, 243, 434, 1835, 2466, 3619
MspR9I	CCNGG	8 cut(s)	86, 146, 2565, 2589, 3333, 3619, 3620, 4048
MunI	CAATTG	4 cut(s)	1458, 3093, 3804, 4095
Mva1269I	GAATGC	1 cut(s)	3861
MvaI	CCWGG	6 cut(s)	86, 146, 2565, 2589, 3333, 4048
MvnI	CGCG	2 cut(s)	27, 2620
NciI	CCSGG	2 cut(s)	3619, 3620
NcoI	CCATGG	1 cut(s)	3012
NdeII	GATC	9 cut(s)	90, 601, 1087, 1689, 3133, 3273, 3443, 3799, 3963
NheI	GCTAGC	1 cut(s)	1453
NmuCI	GTSAC	4 cut(s)	32, 2694, 3335, 3604
NsiI	ATGCAT	1 cut(s)	2670
NspI	RCATGY	3 cut(s)	1520, 3157, 3299
NspV	TTCGAA	2 cut(s)	203, 860
PaeI	GCATGC	1 cut(s)	3157
PagI	TCATGA	3 cut(s)	2245, 3124, 3537
PasI	CCCWGGG	1 cut(s)	2588
PceI	AGGCCT	2 cut(s)	3081, 3695
PciI	ACATGT	1 cut(s)	1516
PcsI	WCGNNNNNNNCGW	3 cut(s)	99, 108, 329
PctI	GAATGC	1 cut(s)	3861
PdmI	GAANNNNTTC	2 cut(s)	323, 2967
PfeI	GAWTC	8 cut(s)	43, 1165, 1244, 2006, 2045, 2257, 2339, 2968
PflMI	CCANNNNNTGG	1 cut(s)	1298
PfoI	TCCNGGA	2 cut(s)	144, 4046
PleI	GAGTC	6 cut(s)	144, 1345, 1717, 2605, 2701, 3844
PpsI	GAGTC	6 cut(s)	144, 1345, 1717, 2605, 2701, 3844
PpuMI	RGGWCCY	3 cut(s)	2560, 3161, 3622
PscI	ACATGT	1 cut(s)	1516
Psp5II	RGGWCCY	3 cut(s)	2560, 3161, 3622
Psp6I	CCWGG	6 cut(s)	84, 144, 2563, 2587, 3331, 4046
PspFI	CCCAGC	2 cut(s)	986, 2109
PspGI	CCWGG	6 cut(s)	84, 144, 2563, 2587, 3331, 4046
PspOMI	GGGCCC	1 cut(s)	3000
PspPPI	RGGWCCY	3 cut(s)	2560, 3161, 3622
PstI	CTGCAG	4 cut(s)	973, 1348, 2203, 2509
PstNI	CAGNNNCTG	3 cut(s)	1409, 2207, 2342
PsuI	RGATCY	4 cut(s)	1087, 1689, 3443, 3799
PvuII	CAGCTG	4 cut(s)	578, 875, 1496, 3737
RsaI	GTAC	7 cut(s)	1809, 2837, 2900, 2940, 3052, 3522, 4065
RsaNI	GTAC	7 cut(s)	1808, 2836, 2899, 2939, 3051, 3521, 4064
Sau3AI	GATC	9 cut(s)	90, 601, 1087, 1689, 3133, 3273, 3443, 3799, 3963
SchI	GAGTC	6 cut(s)	145, 1346, 1717, 2606, 2702, 3844
ScrFI	CCNGG	8 cut(s)	86, 146, 2565, 2589, 3333, 3619, 3620, 4048
SduI	GDGCHC	5 cut(s)	691, 1023, 2595, 3004, 3765
SexAI	ACCWGGT	1 cut(s)	84
SfcI	CTRYAG	9 cut(s)	870, 969, 1344, 1410, 1754, 2199, 2505, 3924, 4078
SfuI	TTCGAA	2 cut(s)	203, 860
SinI	GGWCC	4 cut(s)	142, 2560, 3161, 3622
SmaI	CCCGGG	1 cut(s)	3620
SmlI	CTYRAG	7 cut(s)	350, 1140, 1619, 1634, 1985, 3764, 4067
SmoI	CTYRAG	7 cut(s)	350, 1140, 1619, 1634, 1985, 3764, 4067
SpeI	ACTAGT	1 cut(s)	1043
SphI	GCATGC	1 cut(s)	3157
SseBI	AGGCCT	2 cut(s)	3081, 3695
SspI	AATATT	1 cut(s)	3490
SspMI	CTAG	6 cut(s)	171, 1044, 1178, 1454, 1545, 3440
StuI	AGGCCT	2 cut(s)	3081, 3695
StyD4I	CCNGG	8 cut(s)	84, 144, 2563, 2587, 3331, 3617, 3618, 4046
StyI	CCWWGG	6 cut(s)	213, 639, 2817, 3012, 3082, 3447
TaaI	ACNGT	5 cut(s)	889, 1333, 2933, 3055, 3782
TaiI	ACGT	1 cut(s)	326
TatI	WGTACW	4 cut(s)	2835, 2898, 3520, 4063
TauI	GCSGC	5 cut(s)	30, 677, 1397, 2781, 3153
TfiI	GAWTC	8 cut(s)	43, 1165, 1244, 2006, 2045, 2257, 2339, 2968
TscAI	CASTG	9 cut(s)	286, 820, 928, 1803, 2962, 3002, 3053, 3316, 3553
TseFI	GTSAC	4 cut(s)	32, 2694, 3335, 3604
Tsp45I	GTSAC	4 cut(s)	32, 2694, 3335, 3604
TspGWI	ACGGA	3 cut(s)	100, 2507, 3744
TspMI	CCCGGG	1 cut(s)	3618
TspRI	CASTG	9 cut(s)	286, 820, 928, 1803, 2962, 3002, 3053, 3316, 3553
Van91I	CCANNNNNTGG	1 cut(s)	1298
Vha464I	CTTAAG	2 cut(s)	1619, 4067
VpaK11BI	GGWCC	4 cut(s)	142, 2560, 3161, 3622
XagI	CCTNNNNNAGG	1 cut(s)	174
XapI	RAATTY	6 cut(s)	443, 2332, 2608, 2726, 3042, 3976
XceI	RCATGY	3 cut(s)	1520, 3157, 3299
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2405
XmaI	CCCGGG	1 cut(s)	3618
XmnI	GAANNNNTTC	2 cut(s)	323, 2967
XspI	CTAG	6 cut(s)	171, 1044, 1178, 1454, 1545, 3440
Zsp2I	ATGCAT	1 cut(s)	2670

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.