Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0002582

Physical Location & Seq

Genomic Coordinates Reverse (-)

31590760 .. 31597352

Gene Structure 6593 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000013444

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4155 bp

ATGGGTCGGAAGAAGCCGACTGCTCGTGATGAAGAGAATCCGCCGCCAGCGGGTGGTGGTGGAGGAAAATCTAAGAAGAAGAACCTGGTGATCGAGGACGACGAGTATTCCGTCGGAACTGAGCTATCCGAGGAGAGTCAGGTCAAGGAGGAGAAGGTTCCTCCGCCTAGTAAAGGGAAGAAGGGCAAGAAGGGGAGTTCTAAAGCTTCTAAGGGAGTGGATGAGGACTTGGACGATGCCGATGAGGATGCAGATGAGGTTCCCCAAGTTGTTTTCACTGGGAAGAAGAAGGGCAAGTCGAAGAAGAGTGGCGGGAGTAGCGCGTTCAGCTCGTCCAGTTTCGGGTTGCTTGAGGATGAGGATGATAAGGATACTGAGGATGATGAGCAATCTGGTTTAACAGGTGATGATGAGGAGGAGGATGCTCCGGCGTTGAAATTTTCGGGGAAGAAGAAGCCTTCAAAGTCGACCAAGAAAACCGGGGTTAGTTCGTTTACAGGGTCGGCTTTTGATGCCATTGGTGACGAAGGGGATAGTGATGGCGGGGTTGATGATGAGAGTAATGCAGCTGAAGATCATGAGAATGACGATGAGCCTGCAATTGCTTTTACAGGGAAGAAGAAGGCTTCCAAGGGTGGAAAGAAGGGTGGGAGTGTGTTTGCAGCAGCCAGCTTTAGTGCCCTTGATGATGCGGAGGAGGATAACGATGAAAAAAACGATGAGGATGATGATATTCCTCAGATTACTTTCTCTGGTAAGAAAAAGAAGTCATCAAAGGCTTCAAAGAAGAGTGGCGGTAATTCTTTTAGCGCAGCATTGCTTGATGAGGAAGATGATGAAGATACTTCCGTTTCCAAACCAACTAGTGCCGGTGATGATACAGTTGAGGATGAAGATGCATCTATGATAACATTCACAGGTAAGAAGAAGTCTTCAAAGAAGAAAGGTAATAGTGTGTTTACTGCACTGGGTGAAGATGCTGGGGTTGGGAGTGAAATTACAGATGTAGTTGACCCCAAACAACCAAGTACGGGAACTAGTAAAATTGAAGCTGATAATTCTAACGTTAGTAAGAATAAAGATCCAGAACCTTCAAAAAGTAAGAAAAAGAAGAAGAAGAGTGGAAGGACTGCTCAAGAAGAGGATGATTTGGATAAGATTCTTGCAGAGCTAGGTGAGCTTTCATCGAAACCTGCTGCTCCTCAGAAAGAGGAGACGGTGGAGGTTCAGCCTGCTGATTCTCAGAAAGAGGAGAAAGTTGAGGTTCAACCTGATGTGGTTGCCCCAGTTGATGGTTCTGCTGCAAAGGAAGCTGAAGAAGAGAGTGTGGAGTCTGCTGCAGCAAAGAAGAAGAAAAAGAAGAAGGAAAAGGAAAAGGAGAAAAAGGCGGCAGCGGCAGCAGCAGCGGCATCTGTAGATGTCAAGGAGGAAAAACAAGAACAAGCAAAAATTGAGCCAGCAATTGAACCAAAGAAGAAGGAAGTGAAAGGCAAAGCAGCTGAAAAGAAGGTGCCTAAACATGTTAGGGAGATGCAAGAGGCCCTTGCTAGGCGACAGGAAGAAGAAGAGAAGAAGAAAAGGGAAGAAGAGGAGAAACTGAAGAAAGAAGAAGAGGAGAGGCATAAGCAGGAAGAACTTGAGAAGCAAAAGGAGGAGGCAAAGCGGAAGAAAAAGGAGAGGGAAAAGGAGAAGATCCAAGAGTTGAAGAAGGCAGGTTTATATAAAACTCCCAAGCAAAAAGAAGAGGAGAAGCGGCGGGAGCAAAAGAGACTCCAGTTACTTAGGGATTTACCTAATGCAGTGGAGGGTGTGCCTCCTCCTACCACAGACAATGAAAAACCGGCTAAAAGACCCTTGTATCAGAAAAAGAAGTCAAAACCAGCACCCAATCAGGCAAATGGTGCAGGTACTACAAAGCCTGTGGAAAGCACAGAAGGAATAAAAGAAGAAAACCAGCAGGATACTGTTCCTGAGCTGGATTCTGTGGGACTTGAGAAGGATGAAGAGCAGGAATCAGTTGAAGTAGCAGAAAAGCTATCTGAAGTTTCTGAATCTGTTAAAGAGAATGGATTGGAACAAGAAGAGGATGATGGTGATGATGATGAATGGGATGCAAAGAGCTGGGATGATGATGCTGTTGTTAATCTTTCATTTAAACGTGGGTTTTCTGATGAAGAGGTTGAACCTGTTGTGAATAAAGGGATGAAGCCTGCAGTGCAGAAAACCGTTCCTTCTCAACCAATAAAACCTCATGATGTTGAGAATCAGAAAAAACAAGCTGAGGTGGAAGTTGACAAAGGTAGGAAGAAAGTTGCTACTGCAAAGAAAGAAGCACCAAATTCAGATTCTGCCTCTAAACAGAGTGAAGATAATCTTCGCTCTCCCATTTGCTGCATTATGGGCCATGTTGATACTGGTAAAACTAAGCTTCTGGATTGTATCCGAGGCACAAATGTTCAGGAGGGTGAGGCTGGAGGTATTACTCAACAGATTGGTGCTACGTATTTTCCTGCGGATAATATCCGTGAGAGGACTAAGGAACTGAAAGCTGATGCAAAGCTGAAGGTCCCAGGTCTATTGGTCATTGATACCCCTGGCCACGAGTCATTTACTAATTTAAGGTCACGGGGTTCAGGTTTATGTGATATTGCAATTTTGGTTGTTGACATTATGCATGGCTTAGAGCCTCAAACAATAGAGTCACTCAATCTCTTGAAAATGAGGAATACCGAATTCATTGTTGCATTGAATAAGGTGGACAGGCTCTATGGGTGGAAAACCTGCCGCAACCAGCCAATAGGGAAGGCAATGAAGCAACAATCCAAGGATGTAGTAAATGAGTTCAATATGAGGGTTTCTCAGATTATCACTCAATTCATGGAGCAGGGCTTAAATACTGAATTGTATTATAAGAATAAAGAAATGGGAGAAACATACAGTATTGTACCTACAAGTGCCATTAGTGGTGAAGGGATTCCAGATTTGTTATTACTATTGGTTCAGTGGGCCCAGAAAACTATGGTTGAGAAACTTACTTACAGCAATGAAATTCAGTGTACGGTATTGGAGGTCAAGGTTATTGAAGGTCTTGGGACAACAATCGATGTTGTGTTGGTTAATGGTGTGCTTCATGAAGGGGATCAAATAGTCGTTTGCGGCATGCAGGGGCCTATTGTTACTTCAATTCGAGCTTTGTTGACACCACATCCCATGAAAGAACTTCGTGTCAAGGGAACTTATCAGCACCATAGTCAAATCAAAGCTGCACAAGGTATCAAGATCACAGCACAGGGACTTGAACATGCTATTGCTGGCACTGCTCTATATGTTGTTGGGCCTAGCGATGACTTGGATGATATCAAGGAAACAGCTATGGAAGATATGAAATCAGTCATGAATAGGATTGACAAGAGTGGTGAGGGAGTTTGTGTACAAGCATCTACTCTAGGATCCTTGGAAGCATTGCTAGAGTTTTTGAAGACGCCAGAAGTTAATATTCCTGTTAGTGGTATTAGCATCGGCCCTGTACACAAGAAGGATGTCATGAAGGCCAGTGTAATGCTTGAAAAGAAAAAAGAGTACGCCACTATCTTGGCATTTGATGTTAAAGTGACACCTGAGGCCCGGGACCTTGCAGATGAATTGGGTGTGAAGATTTTTATTGCTGATATAATCTATCACTTATTTGACCAATTTAAGGCCTATATCGACAATCTCAAAGAGGAAAAGAAGAAGGAAGCCGCTGATGAAGCTGTTTTTCCGTGTGTGCTCAAGATTTTACCCAACTGTGTTTTCAACAAGAAGGATCCAATTGTGTTGGGTGTTGATGTTCTTGAAGGCATTGCAAAGGTAGGGACTCCAATTTGCATTCCTCAGAGAGATTTTATTGATATTGGACGCATTGCATCCATTGAGAATAACCACAAACCTGTAGATATAGCGAAAAAGGGGCTGAAGGTGGCTATCAAGATAGTCGGCACAAATTCTGATGAGCAGCAAAAGATGTATGGTAGGCATTTTGAAATTGAAGATGAGCTTGTCAGCCATATCTCCAGGAGGTCAATTGATGTACTTAAAACAAGCTACCGGGATGAACTGTCAATTGACGAGTGGAAGCTGGTTGTGAAATTGAAGAAACTTTTTGAGATACCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001882 GO:0001883 GO:0002181 GO:0002183 GO:0003674 GO:0003676 GO:0003723 GO:0003743 GO:0003824 GO:0003924 GO:0005488 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006412 GO:0006413 GO:0006518 GO:0006807 GO:0008135 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019001 GO:0019538 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032991 GO:0033290 GO:0034641 GO:0034645 GO:0035639 GO:0036094 GO:0043043 GO:0043167 GO:0043168 GO:0043170 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0070993 GO:0071704 GO:0097159 GO:0097367 GO:1901265 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko00195 ko01100 ko03013 map00195 map01100 map03013

Pfam Domains

Protein Families

GTP_EFTU GTP_EFTU_D2 IF-2

Protein Analysis

1385

Amino Acids

151.85

Weight (kDa)

5.2

Isoelectric Point (pI)

40.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GTP_EFTU	PF00009	795 - 1004	4.3e-34	Elongation factor Tu GTP binding domain
IF-2	PF11987	1121 - 1233	3.7e-23	Translation-initiation factor 2
GTP_EFTU_D4	PF14578	1254 - 1327	1.5e-14	Elongation factor Tu domain 4

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002582)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G21160 AT1G76720 AT1G76720 AT1G76810 AT2G27700 AT2G27700
fragaria_vesca	FvH4_2g13940 FvH4_2g13940 FvH4_2g13940 FvH4_4g36100 FvH4_4g36312
malus_domestica	MD05G1023200.v1.1 MD09G1087100.v1.1 MD10G1024100.v1.1
prunus_persica	Prupe.2G099300_v2.0.a1 Prupe.8G027900_v2.0.a1
pyrus_communis	pycom05g01470 pycom09g01240 pycom10g01600
rosa_chinensis	RchiOBHm_Chr4g0445721 RchiOBHm_Chr6g0275701
rosa_laevigata	RLG00000005714 RLG00000013337 RLG00000013444
rosa_multiflora	Rmu_co8469881.1_g000001 Rmu_sc0003637.1_g000011 Rmu_sc0003637.1_g000012 Rmu_sc0007122.1_g000003
rosa_roxburghii	Rroxscaffold_7G00193000
rosa_rugosa	Rorug04G0365200 Rorug06G0095700
rosa_samantha	Rh4AG426100 Rh4CG453100 Rh4DG434300 Rh6AG207600 Rh6BG210300 Rh6CG214100 Rh6DG203400
rosa_wichuraiana	Rw4G036490 Rw6G018100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2910
Acc36I	ACCTGC	4 cut(s)	1201, 1703, 1895, 2789
AccB1I	GGYRCC	1 cut(s)	1510
AccB7I	CCANNNNNTGG	2 cut(s)	53, 1292
AccI	GTMKAC	1 cut(s)	467
AccII	CGCG	1 cut(s)	323
AclI	AACGTT	1 cut(s)	1065
AclWI	GGATC	7 cut(s)	1076, 1687, 3147, 3444, 3457, 3800, 3813
AcoI	YGGCCR	1 cut(s)	2596
AcsI	RAATTY	5 cut(s)	437, 2338, 2732, 3048, 3982
AcuI	CTGAAG	6 cut(s)	591, 1335, 1619, 2061, 2582, 3974
AcyI	GRCGYC	1 cut(s)	3482
AdeI	CACNNNGTG	1 cut(s)	971
AfaI	GTAC	8 cut(s)	1030, 1909, 2946, 3058, 3432, 3528, 3581, 4071
AfiI	CCNNNNNNNGG	9 cut(s)	50, 53, 173, 342, 1031, 1292, 1548, 3506, 3697
AflIII	ACRYGT	1 cut(s)	1519
AhlI	ACTAGT	2 cut(s)	863, 1037
AjnI	CCWGG	4 cut(s)	84, 2569, 2593, 4052
AjuI	GAANNNNNNNTTGG	4 cut(s)	2329, 2361, 3776, 3808
Alw21I	GWGCWC	1 cut(s)	3771
Alw26I	GTCTC	2 cut(s)	1208, 1762
AlwI	GGATC	7 cut(s)	1076, 1687, 3147, 3444, 3457, 3800, 3813
AlwNI	CAGNNNCTG	1 cut(s)	2348
Ama87I	CYCGRG	1 cut(s)	3624
ApaI	GGGCCC	1 cut(s)	3010
ApoI	RAATTY	5 cut(s)	437, 2338, 2732, 3048, 3982
Asp700I	GAANNNNTTC	1 cut(s)	2973
AspLEI	GCGC	2 cut(s)	323, 812
AsuC2I	CCSGG	4 cut(s)	481, 3625, 3626, 4088
AvaI	CYCGRG	1 cut(s)	3624
AvaII	GGWCC	2 cut(s)	2566, 3628
AxyI	CCTNAGG	1 cut(s)	3618
BaeGI	GKGCMC	2 cut(s)	682, 3010
BaeI	ACNNNNGTAYC	4 cut(s)	834, 867, 1841, 1874
BalI	TGGCCA	1 cut(s)	2598
BamHI	GGATCC	2 cut(s)	3449, 3805
BanI	GGYRCC	1 cut(s)	1510
BanII	GRGCYC	1 cut(s)	3010
BauI	CACGAG	2 cut(s)	24, 2600
BbsI	GAAGAC	2 cut(s)	924, 3485
Bbv12I	GWGCWC	1 cut(s)	3771
BbvCI	CCTCAGC	1 cut(s)	2280
BccI	CCATC	3 cut(s)	533, 1286, 2084
BcgI	CGANNNNNNTGC	4 cut(s)	230, 264, 578, 612
BciT130I	CCWGG	4 cut(s)	86, 2571, 2595, 4054
BciVI	GTATCC	3 cut(s)	364, 1954, 2450
BcnI	CCSGG	4 cut(s)	481, 3625, 3626, 4088
BcoDI	GTCTC	2 cut(s)	1208, 1762
BcuI	ACTAGT	2 cut(s)	863, 1037
BfaI	CTAG	8 cut(s)	168, 864, 1038, 1172, 1548, 3339, 3446, 3467
BfmI	CTRYAG	4 cut(s)	1338, 1413, 2211, 3930
BfuAI	ACCTGC	4 cut(s)	1201, 1703, 1895, 2789
BfuI	GTATCC	3 cut(s)	364, 1954, 2450
Bme1390I	CCNGG	8 cut(s)	86, 481, 2571, 2595, 3625, 3626, 4054, 4088
Bme18I	GGWCC	2 cut(s)	2566, 3628
BmeT110I	CYCGRG	1 cut(s)	3624
BmiI	GGNNCC	9 cut(s)	159, 261, 1512, 2568, 3008, 3168, 3451, 3629, 3807
BmrFI	CCNGG	8 cut(s)	86, 481, 2571, 2595, 3625, 3626, 4054, 4088
BmrI	ACTGGG	3 cut(s)	288, 977, 1280
BmuI	ACTGGG	3 cut(s)	288, 977, 1280
BpiI	GAAGAC	2 cut(s)	924, 3485
BplI	GAGNNNNNCTC	2 cut(s)	2842, 2874
BpmI	CTGGAG	3 cut(s)	1757, 2493, 4036
Bpu10I	CCTNAGC	2 cut(s)	1971, 2280
BpuEI	CTTGAG	5 cut(s)	371, 1119, 1658, 2012, 3755
BpuMI	CCSGG	4 cut(s)	481, 3625, 3626, 4088
Bsa29I	ATCGAT	1 cut(s)	3102
BsaAI	YACGTR	1 cut(s)	2502
BsaHI	GRCGYC	1 cut(s)	3482
BsaJI	CCNNGG	9 cut(s)	129, 480, 630, 2443, 2569, 2593, 2823, 3453, 3624
BsaXI	ACNNNNNCTCC	2 cut(s)	3414, 3444
Bsc4I	CCNNNNNNNGG	9 cut(s)	50, 53, 173, 342, 1031, 1292, 1548, 3506, 3697
Bse118I	RCCGGY	2 cut(s)	869, 1840
Bse1I	ACTGG	7 cut(s)	283, 336, 972, 1286, 1774, 2419, 3552
Bse21I	CCTNAGG	1 cut(s)	3618
Bse3DI	GCAATG	6 cut(s)	815, 2814, 3049, 3461, 3840, 3900
BseBI	CCWGG	4 cut(s)	86, 2571, 2595, 4054
BseCI	ATCGAT	1 cut(s)	3102
BseDI	CCNNGG	9 cut(s)	129, 480, 630, 2443, 2569, 2593, 2823, 3453, 3624
BseLI	CCNNNNNNNGG	9 cut(s)	50, 53, 173, 342, 1031, 1292, 1548, 3506, 3697
BseMI	GCAATG	6 cut(s)	815, 2814, 3049, 3461, 3840, 3900
BseNI	ACTGG	7 cut(s)	283, 336, 972, 1286, 1774, 2419, 3552
BseSI	GKGCMC	2 cut(s)	682, 3010
BseYI	CCCAGC	2 cut(s)	980, 2121
BsgI	GTGCAG	4 cut(s)	948, 1923, 2237, 3249
Bsh1236I	CGCG	1 cut(s)	323
BshNI	GGYRCC	1 cut(s)	1510
BshVI	ATCGAT	1 cut(s)	3102
BsiHKAI	GWGCWC	1 cut(s)	3771
BsiHKCI	CYCGRG	1 cut(s)	3624
BsiSI	CCGG	6 cut(s)	428, 480, 870, 1841, 3625, 4087
BslFI	GGGAC	6 cut(s)	2001, 2552, 3106, 3306, 3641, 3868
BslI	CCNNNNNNNGG	9 cut(s)	50, 53, 173, 342, 1031, 1292, 1548, 3506, 3697
BsmAI	GTCTC	2 cut(s)	1208, 1762
BsmBI	CGTCTC	1 cut(s)	1208
BsmFI	GGGAC	6 cut(s)	2001, 2552, 3106, 3306, 3641, 3868
BsmI	GAATGC	1 cut(s)	3867
BsoBI	CYCGRG	1 cut(s)	3624
Bsp120I	GGGCCC	1 cut(s)	3006
Bsp1286I	GDGCHC	3 cut(s)	682, 3010, 3771
Bsp1407I	TGTACA	2 cut(s)	3430, 3526
Bsp143I	GATC	8 cut(s)	90, 574, 1081, 1692, 3139, 3279, 3449, 3805
BspDI	ATCGAT	1 cut(s)	3102
BspFNI	CGCG	1 cut(s)	323
BspHI	TCATGA	5 cut(s)	577, 2251, 3130, 3393, 3543
BspLI	GGNNCC	9 cut(s)	159, 261, 1512, 2568, 3008, 3168, 3451, 3629, 3807
BspMAI	CTGCAG	2 cut(s)	1342, 2215
BspMI	ACCTGC	4 cut(s)	1201, 1703, 1895, 2789
BspPI	GGATC	7 cut(s)	1076, 1687, 3147, 3444, 3457, 3800, 3813
BspQI	GCTCTTC	1 cut(s)	1998
BspT107I	GGYRCC	1 cut(s)	1510
BsrDI	GCAATG	6 cut(s)	815, 2814, 3049, 3461, 3840, 3900
BsrFI	RCCGGY	2 cut(s)	869, 1840
BsrGI	TGTACA	2 cut(s)	3430, 3526
BsrI	ACTGG	7 cut(s)	283, 336, 972, 1286, 1774, 2419, 3552
BssAI	RCCGGY	2 cut(s)	869, 1840
BssECI	CCNNGG	9 cut(s)	129, 480, 630, 2443, 2569, 2593, 2823, 3453, 3624
BssMI	GATC	8 cut(s)	90, 574, 1081, 1692, 3139, 3279, 3449, 3805
BssNI	GRCGYC	1 cut(s)	3482
BssSI	CACGAG	2 cut(s)	24, 2600
BssT1I	CCWWGG	3 cut(s)	630, 2823, 3453
Bst2BI	CACGAG	2 cut(s)	24, 2600
Bst2UI	CCWGG	4 cut(s)	86, 2571, 2595, 4054
Bst4CI	ACNGT	8 cut(s)	883, 1219, 1966, 2227, 2939, 3061, 3788, 4098
BstACI	GRCGYC	1 cut(s)	3482
BstAPI	GCANNNNNTGC	1 cut(s)	1901
BstAUI	TGTACA	2 cut(s)	3430, 3526
BstBAI	YACGTR	1 cut(s)	2502
BstC8I	GCNNGC	8 cut(s)	48, 597, 670, 1233, 1458, 2211, 3161, 3313
BstENI	CCTNNNNNAGG	1 cut(s)	171
BstFNI	CGCG	1 cut(s)	323
BstHHI	GCGC	2 cut(s)	323, 812
BstKTI	GATC	8 cut(s)	93, 577, 1084, 1695, 3142, 3282, 3452, 3808
BstMAI	GTCTC	2 cut(s)	1208, 1762
BstMBI	GATC	8 cut(s)	90, 574, 1081, 1692, 3139, 3279, 3449, 3805
BstNI	CCWGG	4 cut(s)	86, 2571, 2595, 4054
BstNSI	RCATGY	3 cut(s)	1523, 3163, 3305
BstSCI	CCNGG	8 cut(s)	84, 479, 2569, 2593, 3623, 3624, 4052, 4086
BstSFI	CTRYAG	4 cut(s)	1338, 1413, 2211, 3930
BstSLI	GKGCMC	2 cut(s)	682, 3010
BstSNI	TACGTA	1 cut(s)	2502
BstUI	CGCG	1 cut(s)	323
BstV2I	GAAGAC	2 cut(s)	924, 3485
BstX2I	RGATCY	4 cut(s)	1081, 1692, 3449, 3805
BstXI	CCANNNNNNTGG	1 cut(s)	3592
BstYI	RGATCY	4 cut(s)	1081, 1692, 3449, 3805
Bsu15I	ATCGAT	1 cut(s)	3102
Bsu36I	CCTNAGG	1 cut(s)	3618
BsuI	GTATCC	3 cut(s)	364, 1954, 2450
BsuTUI	ATCGAT	1 cut(s)	3102
BtgZI	GCGATG	1 cut(s)	3357
BtsI	GCAGTG	3 cut(s)	1806, 2220, 3315
BtsIMutI	CAGTG	8 cut(s)	276, 965, 1806, 2220, 3008, 3059, 3315, 3559
BveI	ACCTGC	4 cut(s)	1201, 1703, 1895, 2789
Cac8I	GCNNGC	8 cut(s)	48, 597, 670, 1233, 1458, 2211, 3161, 3313
CaiI	CAGNNNCTG	1 cut(s)	2348
CciI	TCATGA	5 cut(s)	577, 2251, 3130, 3393, 3543
CfoI	GCGC	2 cut(s)	323, 812
Cfr10I	RCCGGY	2 cut(s)	869, 1840
Cfr9I	CCCGGG	1 cut(s)	3624
ClaI	ATCGAT	1 cut(s)	3102
CseI	GACGC	2 cut(s)	3490, 3906
CsiI	ACCWGGT	1 cut(s)	84
Csp6I	GTAC	8 cut(s)	1029, 1908, 2945, 3057, 3431, 3527, 3580, 4070
CspCI	CAANNNNNGTGG	2 cut(s)	1902, 1937
CviQI	GTAC	8 cut(s)	1029, 1908, 2945, 3057, 3431, 3527, 3580, 4070
DpnI	GATC	8 cut(s)	92, 576, 1083, 1694, 3141, 3281, 3451, 3807
DpnII	GATC	8 cut(s)	90, 574, 1081, 1692, 3139, 3279, 3449, 3805
DraI	TTTAAA	1 cut(s)	2155
DraIII	CACNNNGTG	1 cut(s)	971
EaeI	YGGCCR	1 cut(s)	2596
EciI	GGCGGA	2 cut(s)	30, 153
Eco105I	TACGTA	1 cut(s)	2502
Eco130I	CCWWGG	3 cut(s)	630, 2823, 3453
Eco147I	AGGCCT	1 cut(s)	3701
Eco24I	GRGCYC	1 cut(s)	3010
Eco32I	GATATC	1 cut(s)	3358
Eco47I	GGWCC	2 cut(s)	2566, 3628
Eco57I	CTGAAG	6 cut(s)	591, 1335, 1619, 2061, 2582, 3974
Eco81I	CCTNAGG	1 cut(s)	3618
Eco88I	CYCGRG	1 cut(s)	3624
EcoNI	CCTNNNNNAGG	1 cut(s)	171
EcoO109I	RGGNCCY	4 cut(s)	1540, 2566, 3167, 3628
EcoRI	GAATTC	1 cut(s)	2732
EcoRII	CCWGG	4 cut(s)	84, 2569, 2593, 4052
EcoRV	GATATC	1 cut(s)	3358
EcoT14I	CCWWGG	3 cut(s)	630, 2823, 3453
EcoT22I	ATGCAT	2 cut(s)	901, 2676
EcoT38I	GRGCYC	1 cut(s)	3010
ErhI	CCWWGG	3 cut(s)	630, 2823, 3453
Esp3I	CGTCTC	1 cut(s)	1208
FalI	AAGNNNNNCTT	6 cut(s)	1527, 1559, 2358, 2390, 4020, 4052
FaqI	GGGAC	6 cut(s)	2001, 2552, 3106, 3306, 3641, 3868
FauI	CCCGC	4 cut(s)	43, 305, 536, 1749
FblI	GTMKAC	1 cut(s)	467
FriOI	GRGCYC	1 cut(s)	3010
FspBI	CTAG	8 cut(s)	168, 864, 1038, 1172, 1548, 3339, 3446, 3467
GlaI	GCGC	2 cut(s)	322, 811
GsaI	CCCAGC	2 cut(s)	984, 2125
GsuI	CTGGAG	3 cut(s)	1757, 2493, 4036
HapII	CCGG	6 cut(s)	428, 480, 870, 1841, 3625, 4087
HgaI	GACGC	2 cut(s)	3490, 3906
HhaI	GCGC	2 cut(s)	323, 812
Hin1I	GRCGYC	1 cut(s)	3482
Hin6I	GCGC	2 cut(s)	321, 810
HinP1I	GCGC	2 cut(s)	321, 810
HincII	GTYRAC	5 cut(s)	468, 1012, 2293, 2665, 3198
HindII	GTYRAC	5 cut(s)	468, 1012, 2293, 2665, 3198
HindIII	AAGCTT	2 cut(s)	204, 2426
HpaII	CCGG	6 cut(s)	428, 480, 870, 1841, 3625, 4087
Hpy99I	CGWCG	2 cut(s)	104, 116
HpyCH4III	ACNGT	8 cut(s)	883, 1219, 1966, 2227, 2939, 3061, 3788, 4098
HpyCH4IV	ACGT	3 cut(s)	1065, 2158, 2501
HpySE526I	ACGT	3 cut(s)	1065, 2158, 2501
Hsp92I	GRCGYC	1 cut(s)	3482
HspAI	GCGC	2 cut(s)	321, 810
Kzo9I	GATC	8 cut(s)	90, 574, 1081, 1692, 3139, 3279, 3449, 3805
LguI	GCTCTTC	1 cut(s)	1998
LmnI	GCTCC	4 cut(s)	430, 1204, 1759, 2881
MabI	ACCWGGT	1 cut(s)	84
MaeI	CTAG	8 cut(s)	168, 864, 1038, 1172, 1548, 3339, 3446, 3467
MaeII	ACGT	3 cut(s)	1065, 2158, 2501
MaeIII	GTNAC	6 cut(s)	521, 1776, 2622, 2700, 3175, 3610
MalI	GATC	8 cut(s)	92, 576, 1083, 1694, 3141, 3281, 3451, 3807
MboI	GATC	8 cut(s)	90, 574, 1081, 1692, 3139, 3279, 3449, 3805
MfeI	CAATTG	5 cut(s)	600, 1461, 3810, 4062, 4101
MflI	RGATCY	4 cut(s)	1081, 1692, 3449, 3805
MhlI	GDGCHC	3 cut(s)	682, 3010, 3771
MlsI	TGGCCA	1 cut(s)	2598
MluNI	TGGCCA	1 cut(s)	2598
MlyI	GAGTC	6 cut(s)	145, 1340, 1764, 2612, 2708, 3850
MmeI	TCCRAC	1 cut(s)	94
Mox20I	TGGCCA	1 cut(s)	2598
Mph1103I	ATGCAT	2 cut(s)	901, 2676
MroXI	GAANNNNTTC	1 cut(s)	2973
MscI	TGGCCA	1 cut(s)	2598
MslI	CAYNNNNRTG	1 cut(s)	582
Msp20I	TGGCCA	1 cut(s)	2598
MspA1I	CMGCKG	6 cut(s)	50, 569, 1394, 1406, 1499, 3743
MspI	CCGG	6 cut(s)	428, 480, 870, 1841, 3625, 4087
MspR9I	CCNGG	8 cut(s)	86, 481, 2571, 2595, 3625, 3626, 4054, 4088
MunI	CAATTG	5 cut(s)	600, 1461, 3810, 4062, 4101
Mva1269I	GAATGC	1 cut(s)	3867
MvaI	CCWGG	4 cut(s)	86, 2571, 2595, 4054
MvnI	CGCG	1 cut(s)	323
NciI	CCSGG	4 cut(s)	481, 3625, 3626, 4088
NdeII	GATC	8 cut(s)	90, 574, 1081, 1692, 3139, 3279, 3449, 3805
NlaIV	GGNNCC	9 cut(s)	159, 261, 1512, 2568, 3008, 3168, 3451, 3629, 3807
NmuCI	GTSAC	4 cut(s)	521, 2622, 2700, 3610
NsiI	ATGCAT	2 cut(s)	901, 2676
NspI	RCATGY	3 cut(s)	1523, 3163, 3305
PaeI	GCATGC	1 cut(s)	3163
PagI	TCATGA	5 cut(s)	577, 2251, 3130, 3393, 3543
PceI	AGGCCT	1 cut(s)	3701
PciI	ACATGT	1 cut(s)	1519
PciSI	GCTCTTC	1 cut(s)	1998
PcsI	WCGNNNNNNNCGW	2 cut(s)	99, 108
PctI	GAATGC	1 cut(s)	3867
PdmI	GAANNNNTTC	1 cut(s)	2973
PfeI	GAWTC	9 cut(s)	37, 1159, 1238, 1979, 2012, 2051, 2263, 2345, 2974
PflMI	CCANNNNNTGG	2 cut(s)	53, 1292
PfoI	TCCNGGA	1 cut(s)	4052
PleI	GAGTC	6 cut(s)	144, 1339, 1764, 2611, 2707, 3850
PpsI	GAGTC	6 cut(s)	144, 1339, 1764, 2611, 2707, 3850
Ppu21I	YACGTR	1 cut(s)	2502
PpuMI	RGGWCCY	2 cut(s)	2566, 3628
PscI	ACATGT	1 cut(s)	1519
PsiI	TTATAA	1 cut(s)	2910
Psp1406I	AACGTT	1 cut(s)	1065
Psp5II	RGGWCCY	2 cut(s)	2566, 3628
Psp6I	CCWGG	4 cut(s)	84, 2569, 2593, 4052
PspFI	CCCAGC	2 cut(s)	980, 2121
PspGI	CCWGG	4 cut(s)	84, 2569, 2593, 4052
PspN4I	GGNNCC	9 cut(s)	159, 261, 1512, 2568, 3008, 3168, 3451, 3629, 3807
PspOMI	GGGCCC	1 cut(s)	3006
PspPPI	RGGWCCY	2 cut(s)	2566, 3628
PstI	CTGCAG	2 cut(s)	1342, 2215
PstNI	CAGNNNCTG	1 cut(s)	2348
PsuI	RGATCY	4 cut(s)	1081, 1692, 3449, 3805
PvuII	CAGCTG	2 cut(s)	569, 1499
RsaI	GTAC	8 cut(s)	1030, 1909, 2946, 3058, 3432, 3528, 3581, 4071
RsaNI	GTAC	8 cut(s)	1029, 1908, 2945, 3057, 3431, 3527, 3580, 4070
RseI	CAYNNNNRTG	1 cut(s)	582
SalI	GTCGAC	1 cut(s)	466
SapI	GCTCTTC	1 cut(s)	1998
Sau3AI	GATC	8 cut(s)	90, 574, 1081, 1692, 3139, 3279, 3449, 3805
SchI	GAGTC	6 cut(s)	145, 1340, 1764, 2612, 2708, 3850
ScrFI	CCNGG	8 cut(s)	86, 481, 2571, 2595, 3625, 3626, 4054, 4088
SduI	GDGCHC	3 cut(s)	682, 3010, 3771
SexAI	ACCWGGT	1 cut(s)	84
SfcI	CTRYAG	4 cut(s)	1338, 1413, 2211, 3930
SinI	GGWCC	2 cut(s)	2566, 3628
SmaI	CCCGGG	1 cut(s)	3626
SmiMI	CAYNNNNRTG	1 cut(s)	582
SmlI	CTYRAG	5 cut(s)	350, 1134, 1637, 1991, 3770
SmoI	CTYRAG	5 cut(s)	350, 1134, 1637, 1991, 3770
SnaBI	TACGTA	1 cut(s)	2502
SpeI	ACTAGT	2 cut(s)	863, 1037
SphI	GCATGC	1 cut(s)	3163
SseBI	AGGCCT	1 cut(s)	3701
SspI	AATATT	1 cut(s)	3496
SspMI	CTAG	8 cut(s)	168, 864, 1038, 1172, 1548, 3339, 3446, 3467
StuI	AGGCCT	1 cut(s)	3701
StyD4I	CCNGG	8 cut(s)	84, 479, 2569, 2593, 3623, 3624, 4052, 4086
StyI	CCWWGG	3 cut(s)	630, 2823, 3453
TaaI	ACNGT	8 cut(s)	883, 1219, 1966, 2227, 2939, 3061, 3788, 4098
TaiI	ACGT	3 cut(s)	1068, 2161, 2504
TaqI	TCGA	7 cut(s)	93, 299, 467, 1187, 3102, 3187, 3708
TatI	WGTACW	3 cut(s)	3430, 3526, 4069
TauI	GCSGC	8 cut(s)	46, 1391, 1397, 1409, 1756, 2787, 3159, 3743
TfiI	GAWTC	9 cut(s)	37, 1159, 1238, 1979, 2012, 2051, 2263, 2345, 2974
TscAI	CASTG	8 cut(s)	283, 972, 1806, 2220, 3008, 3059, 3322, 3559
TseFI	GTSAC	4 cut(s)	521, 2622, 2700, 3610
Tsp45I	GTSAC	4 cut(s)	521, 2622, 2700, 3610
TspGWI	ACGGA	4 cut(s)	100, 838, 2513, 3750
TspMI	CCCGGG	1 cut(s)	3624
TspRI	CASTG	8 cut(s)	283, 972, 1806, 2220, 3008, 3059, 3322, 3559
Van91I	CCANNNNNTGG	2 cut(s)	53, 1292
VpaK11BI	GGWCC	2 cut(s)	2566, 3628
XagI	CCTNNNNNAGG	1 cut(s)	171
XapI	RAATTY	5 cut(s)	437, 2338, 2732, 3048, 3982
XceI	RCATGY	3 cut(s)	1523, 3163, 3305
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2411
XmaI	CCCGGG	1 cut(s)	3624
XmiI	GTMKAC	1 cut(s)	467
XmnI	GAANNNNTTC	1 cut(s)	2973
XspI	CTAG	8 cut(s)	168, 864, 1038, 1172, 1548, 3339, 3446, 3467
Zsp2I	ATGCAT	2 cut(s)	901, 2676

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.