Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0002582

Physical Location & Seq

Genomic Coordinates Reverse (-)

11813949 .. 11816002

Gene Structure 2054 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G27700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1440 bp

ATGGATCTGAAGAAGCCGAGTGCTCGAGGTGGAGATGATAAGCAGCAAATTGTTGGCGATGAACACTCTAGACGTAATAAGCTCTCCGAGGAAGACAAAGTCCGTTCTCCCATTTGCTGTATGATGGGTCATATCGATACTGGTAAAACAAAGCTGTTAGATTACATCACAGGAAATGTACAAGAAGGCGAGGCTGGAGGTACCACTCAGAAGATGGGTGCAACATATCTCTCAGCGAGAAACATCCTAGAGAAGACCATGGAACTGAAATCCGATACAAAACTTAAGGTGCCACGTCCACTGTTTATCGATACACCTGGTTACGAGTTCTACACAAATCTTCGCTCAAGAGGCTTAGGTTTGTGTGACTTTGCAATTTTGGTTGTTGACATAATGCATGGGTTAGAGCCACAAACTATAGAATGTCTAAATCTTCTGAGAATGAAGAATACGGAGTTCATTATTGCATTGAATAAGGTGGATAGGCTATATGGATGGAGAATATGTGAAAATGCTCCTATCTTGGAGGCGATGAAGCAACAAACTAAAGATGTTATAAATGAATTTAACATGAGGCTGAATGAGATTATAAACCAGTTTAAGGAGCAAGGACTCAACAGTGAGCTTTATTACAAGAATAAAGAAATGGGAGAAACTTTTAGTATTGTTCCTACTTGTGCTATTAGTGGGGAAGGGATCCCAGATCTATTGTTGTTGTTGGTTCAATTGACTCAGAAAACAATGGTTGAGAAACTAATTACATATGTTGACAAAGTGCAGTGTACTGTCCTAGAGGTCAAAGTTATGGAAGGCTATGGTACAACGATTGATGTTGTGTTGGTAAATGGTGAACTCCATGAAGGTGGTCAAATCGTCGTTTGTGGTCTACAGGGACCTATTGTCACAACGATTAGAGCATTACTGACTCCTCATCCAATAAAGGAGTTGCATGTGAATGGTAATCATGTACATCACGAAGTAATAAAGGCTGCAGAGTGTATCAATATTATTGCCAAGGACCTTGAACATGTAATTGTTGGTACTGCCCTGCATGTCGTTGGACCTGATGATGACATCGAAGCAATTAAGGAGTTGGTTATGGAAGATGTAAACTCAGTTTTGAGCAGGATTGACAAAAGTGGTGAAGGAGTTTATATACAAGCGTCTACATTAGGTTCTTTGGAAGCATTGCTTGAATTCTTGAAGTCCCCAGCTGTAAAGTTACCTGTTGGTGGTATTGGCATAGGGCCTGTGCAAAAGAAGGATGTAATGAAGGCGGGAGTGATGCTCGAGAGGAAAAAGGAATTTGCTACAATTTTGGCTTTAGACGTGGAAGTGACTACAGAGGCTCGTGAACTTGCTGACGAAATGGAAGTTAAAATCTTCTGCTCTGATATCATGTATCATTTATTCGATCAGTATCAGGCATATATATGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00195 ko01100 ko03013 map00195 map01100 map03013

Pfam Domains

Protein Families

GTP_EFTU GTP_EFTU_D2 IF-2

Protein Analysis

479

Amino Acids

53.33

Weight (kDa)

5.28

Isoelectric Point (pI)

28.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GTP_EFTU	PF00009	37 - 245	1.5e-29	Elongation factor Tu GTP binding domain
IF-2	PF11987	367 - 474	4.1e-18	Translation-initiation factor 2

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002582)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G21160 AT1G76720 AT1G76720 AT1G76810 AT2G27700 AT2G27700
fragaria_vesca	FvH4_2g13940 FvH4_2g13940 FvH4_2g13940 FvH4_4g36100 FvH4_4g36312
malus_domestica	MD05G1023200.v1.1 MD09G1087100.v1.1 MD10G1024100.v1.1
prunus_persica	Prupe.2G099300_v2.0.a1 Prupe.8G027900_v2.0.a1
pyrus_communis	pycom05g01470 pycom09g01240 pycom10g01600
rosa_chinensis	RchiOBHm_Chr4g0445721 RchiOBHm_Chr6g0275701
rosa_laevigata	RLG00000005714 RLG00000013337 RLG00000013444
rosa_multiflora	Rmu_co8469881.1_g000001 Rmu_sc0003637.1_g000011 Rmu_sc0003637.1_g000012 Rmu_sc0007122.1_g000003
rosa_roxburghii	Rroxscaffold_7G00193000
rosa_rugosa	Rorug04G0365200 Rorug06G0095700
rosa_samantha	Rh4AG426100 Rh4CG453100 Rh4DG434300 Rh6AG207600 Rh6BG210300 Rh6CG214100 Rh6DG203400
rosa_wichuraiana	Rw4G036490 Rw6G018100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	557, 590
Acc65I	GGTACC	1 cut(s)	200
AccB1I	GGYRCC	2 cut(s)	200, 289
AccI	GTMKAC	2 cut(s)	886, 1166
AciI	CCGC	1 cut(s)	1277
AclWI	GGATC	3 cut(s)	12, 691, 704
AcsI	RAATTY	3 cut(s)	563, 1196, 1304
AcuI	CTGAAG	1 cut(s)	29
AfaI	GTAC	6 cut(s)	180, 202, 784, 820, 969, 1042
AfiI	CCNNNNNNNGG	2 cut(s)	601, 1232
AflII	CTTAAG	1 cut(s)	284
AflIII	ACRYGT	1 cut(s)	1027
AgsI	TTSAA	5 cut(s)	472, 725, 1025, 1196, 1204
AhdI	GACNNNNNGTC	1 cut(s)	899
AjiI	CACGTC	2 cut(s)	296, 1330
AjnI	CCWGG	1 cut(s)	316
AluBI	AGCT	4 cut(s)	82, 154, 625, 1214
AluI	AGCT	4 cut(s)	82, 154, 625, 1214
Alw21I	GWGCWC	1 cut(s)	25
AlwI	GGATC	3 cut(s)	12, 691, 704
Ama87I	CYCGRG	2 cut(s)	24, 1289
AoxI	GGCC	1 cut(s)	1247
ApeKI	GCWGC	2 cut(s)	43, 989
ApoI	RAATTY	3 cut(s)	563, 1196, 1304
Asp718I	GGTACC	1 cut(s)	200
AspS9I	GGNCC	4 cut(s)	893, 1018, 1061, 1247
AsuHPI	GGTGA	2 cut(s)	860, 1154
AvaI	CYCGRG	2 cut(s)	24, 1289
AvaII	GGWCC	3 cut(s)	893, 1018, 1061
BamHI	GGATCC	1 cut(s)	696
BanI	GGYRCC	2 cut(s)	200, 289
BauI	CACGAG	1 cut(s)	1350
BbsI	GAAGAC	2 cut(s)	99, 260
Bbv12I	GWGCWC	1 cut(s)	25
BbvI	GCAGC	2 cut(s)	55, 976
BccI	CCATC	3 cut(s)	118, 208, 489
BciT130I	CCWGG	1 cut(s)	318
BfaI	CTAG	3 cut(s)	69, 248, 791
BfmI	CTRYAG	4 cut(s)	417, 887, 990, 1341
BfrI	CTTAAG	1 cut(s)	284
BglII	AGATCT	1 cut(s)	703
BisI	GCNGC	2 cut(s)	44, 990
BlsI	GCNGC	2 cut(s)	45, 991
Bme1390I	CCNGG	1 cut(s)	318
Bme18I	GGWCC	3 cut(s)	893, 1018, 1061
BmeRI	GACNNNNNGTC	1 cut(s)	899
BmeT110I	CYCGRG	2 cut(s)	24, 1289
BmgBI	CACGTC	2 cut(s)	296, 1330
BmgT120I	GGNCC	4 cut(s)	893, 1018, 1061, 1247
BmiI	GGNNCC	4 cut(s)	202, 291, 698, 894
BmrFI	CCNGG	1 cut(s)	318
BmsI	GCATC	1 cut(s)	1275
BpiI	GAAGAC	2 cut(s)	99, 260
BplI	GAGNNNNNCTC	2 cut(s)	1272, 1304
BpmI	CTGGAG	1 cut(s)	216
Bpu10I	CCTNAGC	1 cut(s)	355
BpuEI	CTTGAG	1 cut(s)	331
Bsa29I	ATCGAT	2 cut(s)	135, 309
BsaBI	GATNNNNATC	1 cut(s)	1419
BsaJI	CCNNGG	3 cut(s)	87, 258, 1014
BsaXI	ACNNNNNCTCC	2 cut(s)	490, 520
Bsc4I	CCNNNNNNNGG	2 cut(s)	601, 1232
Bse1I	ACTGG	2 cut(s)	145, 595
Bse3DI	GCAATG	1 cut(s)	1187
Bse8I	GATNNNNATC	1 cut(s)	1419
BseBI	CCWGG	1 cut(s)	318
BseCI	ATCGAT	2 cut(s)	135, 309
BseDI	CCNNGG	3 cut(s)	87, 258, 1014
BseGI	GGATG	4 cut(s)	243, 500, 931, 1270
BseJI	GATNNNNATC	1 cut(s)	1419
BseLI	CCNNNNNNNGG	2 cut(s)	601, 1232
BseMI	GCAATG	1 cut(s)	1187
BseMII	CTCAG	5 cut(s)	221, 246, 428, 746, 1128
BseNI	ACTGG	2 cut(s)	145, 595
BseRI	GAGGAG	1 cut(s)	918
BseXI	GCAGC	2 cut(s)	55, 976
BseYI	CCCAGC	1 cut(s)	1210
BsgI	GTGCAG	1 cut(s)	797
BshFI	GGCC	1 cut(s)	1249
BshNI	GGYRCC	2 cut(s)	200, 289
BshVI	ATCGAT	2 cut(s)	135, 309
BsiHKAI	GWGCWC	1 cut(s)	25
BsiHKCI	CYCGRG	2 cut(s)	24, 1289
BslFI	GGGAC	2 cut(s)	906, 1192
BslI	CCNNNNNNNGG	2 cut(s)	601, 1232
BsmFI	GGGAC	2 cut(s)	906, 1192
BsnI	GGCC	1 cut(s)	1249
BsoBI	CYCGRG	2 cut(s)	24, 1289
Bsp1286I	GDGCHC	1 cut(s)	25
Bsp1407I	TGTACA	2 cut(s)	178, 967
Bsp143I	GATC	4 cut(s)	4, 696, 703, 1414
Bsp19I	CCATGG	1 cut(s)	258
BspACI	CCGC	1 cut(s)	1277
BspANI	GGCC	1 cut(s)	1249
BspCNI	CTCAG	5 cut(s)	220, 245, 429, 745, 1127
BspDI	ATCGAT	2 cut(s)	135, 309
BspLI	GGNNCC	4 cut(s)	202, 291, 698, 894
BspMAI	CTGCAG	1 cut(s)	994
BspPI	GGATC	3 cut(s)	12, 691, 704
BspT107I	GGYRCC	2 cut(s)	200, 289
BspTI	CTTAAG	1 cut(s)	284
BsrDI	GCAATG	1 cut(s)	1187
BsrGI	TGTACA	2 cut(s)	178, 967
BsrI	ACTGG	2 cut(s)	145, 595
BssECI	CCNNGG	3 cut(s)	87, 258, 1014
BssMI	GATC	4 cut(s)	4, 696, 703, 1414
BssSI	CACGAG	1 cut(s)	1350
BssT1I	CCWWGG	2 cut(s)	258, 1014
Bst2BI	CACGAG	1 cut(s)	1350
Bst2UI	CCWGG	1 cut(s)	318
Bst4CI	ACNGT	3 cut(s)	303, 620, 787
BstAFI	CTTAAG	1 cut(s)	284
BstAUI	TGTACA	2 cut(s)	178, 967
BstDEI	CTNAG	6 cut(s)	207, 232, 355, 437, 732, 1114
BstDSI	CCRYGG	1 cut(s)	258
BstF5I	GGATG	4 cut(s)	243, 500, 931, 1270
BstKTI	GATC	4 cut(s)	7, 699, 706, 1417
BstMBI	GATC	4 cut(s)	4, 696, 703, 1414
BstMWI	GCNNNNNNNGC	1 cut(s)	351
BstNI	CCWGG	1 cut(s)	318
BstNSI	RCATGY	3 cut(s)	953, 1031, 1055
BstSCI	CCNGG	1 cut(s)	316
BstSFI	CTRYAG	4 cut(s)	417, 887, 990, 1341
BstV1I	GCAGC	2 cut(s)	55, 976
BstV2I	GAAGAC	2 cut(s)	99, 260
BstX2I	RGATCY	3 cut(s)	4, 696, 703
BstXI	CCANNNNNNTGG	1 cut(s)	863
BstYI	RGATCY	3 cut(s)	4, 696, 703
Bsu15I	ATCGAT	2 cut(s)	135, 309
BsuRI	GGCC	1 cut(s)	1249
BsuTUI	ATCGAT	2 cut(s)	135, 309
BtgI	CCRYGG	1 cut(s)	258
BtgZI	GCGATG	2 cut(s)	72, 545
BtrI	CACGTC	2 cut(s)	296, 1330
BtsCI	GGATG	4 cut(s)	243, 500, 931, 1270
BtsI	GCAGTG	1 cut(s)	785
BtsIMutI	CAGTG	3 cut(s)	299, 625, 785
Cfr13I	GGNCC	4 cut(s)	893, 1018, 1061, 1247
ClaI	ATCGAT	2 cut(s)	135, 309
CseI	GACGC	1 cut(s)	1152
CsiI	ACCWGGT	1 cut(s)	316
Csp6I	GTAC	6 cut(s)	179, 201, 783, 819, 968, 1041
CviAII	CATG	9 cut(s)	259, 398, 571, 857, 950, 965, 1028, 1052, 1399
CviQI	GTAC	6 cut(s)	179, 201, 783, 819, 968, 1041
DdeI	CTNAG	6 cut(s)	207, 232, 355, 437, 732, 1114
DpnI	GATC	4 cut(s)	6, 698, 705, 1416
DpnII	GATC	4 cut(s)	4, 696, 703, 1414
DriI	GACNNNNNGTC	1 cut(s)	899
Eam1105I	GACNNNNNGTC	1 cut(s)	899
Eco130I	CCWWGG	2 cut(s)	258, 1014
Eco32I	GATATC	1 cut(s)	1396
Eco47I	GGWCC	3 cut(s)	893, 1018, 1061
Eco57I	CTGAAG	1 cut(s)	29
Eco88I	CYCGRG	2 cut(s)	24, 1289
EcoO109I	RGGNCCY	3 cut(s)	893, 1018, 1247
EcoRI	GAATTC	1 cut(s)	1196
EcoRII	CCWGG	1 cut(s)	316
EcoRV	GATATC	1 cut(s)	1396
EcoT14I	CCWWGG	2 cut(s)	258, 1014
EcoT22I	ATGCAT	1 cut(s)	399
ErhI	CCWWGG	2 cut(s)	258, 1014
FaeI	CATG	9 cut(s)	262, 401, 574, 860, 953, 968, 1031, 1055, 1402
FalI	AAGNNNNNCTT	2 cut(s)	1176, 1208
FaqI	GGGAC	2 cut(s)	906, 1192
FatI	CATG	9 cut(s)	258, 397, 570, 856, 949, 964, 1027, 1051, 1398
FauI	CCCGC	1 cut(s)	1270
FauNDI	CATATG	1 cut(s)	763
FblI	GTMKAC	2 cut(s)	886, 1166
Fnu4HI	GCNGC	2 cut(s)	44, 990
FokI	GGATG	4 cut(s)	230, 507, 918, 1277
Fsp4HI	GCNGC	2 cut(s)	44, 990
FspBI	CTAG	3 cut(s)	69, 248, 791
GluI	GCNGC	2 cut(s)	44, 990
GsaI	CCCAGC	1 cut(s)	1214
GsuI	CTGGAG	1 cut(s)	216
HaeIII	GGCC	1 cut(s)	1249
HgaI	GACGC	1 cut(s)	1152
Hin1II	CATG	9 cut(s)	262, 401, 574, 860, 953, 968, 1031, 1055, 1402
HincII	GTYRAC	2 cut(s)	388, 769
HindII	GTYRAC	2 cut(s)	388, 769
HinfI	GANTC	3 cut(s)	612, 730, 925
HphI	GGTGA	2 cut(s)	860, 1154
Hpy166II	GTNNAC	9 cut(s)	299, 388, 769, 783, 851, 887, 1111, 1167, 1355
Hpy188I	TCNGA	7 cut(s)	9, 88, 210, 274, 438, 735, 1393
Hpy188III	TCNNGA	6 cut(s)	69, 348, 974, 1201, 1291, 1352
Hpy8I	GTNNAC	9 cut(s)	299, 388, 769, 783, 851, 887, 1111, 1167, 1355
Hpy99I	CGWCG	1 cut(s)	878
HpyAV	CCTTC	7 cut(s)	179, 686, 803, 854, 1139, 1255, 1267
HpyCH4III	ACNGT	3 cut(s)	303, 620, 787
HpyCH4IV	ACGT	3 cut(s)	73, 295, 1329
HpyCH4V	TGCA	9 cut(s)	221, 374, 397, 467, 778, 949, 992, 1051, 1255
HpyF10VI	GCNNNNNNNGC	1 cut(s)	351
HpyF3I	CTNAG	6 cut(s)	207, 232, 355, 437, 732, 1114
HpySE526I	ACGT	3 cut(s)	73, 295, 1329
Hsp92II	CATG	9 cut(s)	262, 401, 574, 860, 953, 968, 1031, 1055, 1402
KpnI	GGTACC	1 cut(s)	204
Kzo9I	GATC	4 cut(s)	4, 696, 703, 1414
LmnI	GCTCC	2 cut(s)	520, 604
Lsp1109I	GCAGC	2 cut(s)	55, 976
LweI	GCATC	1 cut(s)	1275
MabI	ACCWGGT	1 cut(s)	316
MaeI	CTAG	3 cut(s)	69, 248, 791
MaeII	ACGT	3 cut(s)	73, 295, 1329
MaeIII	GTNAC	5 cut(s)	320, 365, 901, 1221, 1336
MalI	GATC	4 cut(s)	6, 698, 705, 1416
MboI	GATC	4 cut(s)	4, 696, 703, 1414
MboII	GAAGA	9 cut(s)	22, 104, 223, 265, 332, 425, 457, 1115, 1375
MfeI	CAATTG	1 cut(s)	725
MflI	RGATCY	3 cut(s)	4, 696, 703
MhlI	GDGCHC	1 cut(s)	25
MlyI	GAGTC	3 cut(s)	606, 724, 919
MmeI	TCCRAC	1 cut(s)	1039
Mph1103I	ATGCAT	1 cut(s)	399
MseI	TTAA	5 cut(s)	285, 567, 600, 1086, 1377
MslI	CAYNNNNRTG	3 cut(s)	861, 954, 1432
MspA1I	CMGCKG	1 cut(s)	1214
MspCI	CTTAAG	1 cut(s)	284
MspR9I	CCNGG	1 cut(s)	318
MunI	CAATTG	1 cut(s)	725
MvaI	CCWGG	1 cut(s)	318
MwoI	GCNNNNNNNGC	1 cut(s)	351
NcoI	CCATGG	1 cut(s)	258
NdeI	CATATG	1 cut(s)	763
NdeII	GATC	4 cut(s)	4, 696, 703, 1414
NlaIII	CATG	9 cut(s)	262, 401, 574, 860, 953, 968, 1031, 1055, 1402
NlaIV	GGNNCC	4 cut(s)	202, 291, 698, 894
NmeAIII	GCCGAG	1 cut(s)	42
NmuCI	GTSAC	3 cut(s)	365, 901, 1336
NsiI	ATGCAT	1 cut(s)	399
NspI	RCATGY	3 cut(s)	953, 1031, 1055
PaeR7I	CTCGAG	2 cut(s)	24, 1289
PciI	ACATGT	1 cut(s)	1027
PflFI	GACNNNGTC	1 cut(s)	98
PkrI	GCNGC	2 cut(s)	45, 991
PleI	GAGTC	3 cut(s)	606, 724, 919
PpsI	GAGTC	3 cut(s)	606, 724, 919
PpuMI	RGGWCCY	2 cut(s)	893, 1018
PscI	ACATGT	1 cut(s)	1027
PsiI	TTATAA	2 cut(s)	557, 590
Psp5II	RGGWCCY	2 cut(s)	893, 1018
Psp6I	CCWGG	1 cut(s)	316
PspFI	CCCAGC	1 cut(s)	1210
PspGI	CCWGG	1 cut(s)	316
PspN4I	GGNNCC	4 cut(s)	202, 291, 698, 894
PspPI	GGNCC	4 cut(s)	893, 1018, 1061, 1247
PspPPI	RGGWCCY	2 cut(s)	893, 1018
PspXI	VCTCGAGB	1 cut(s)	24
PstI	CTGCAG	1 cut(s)	994
PsuI	RGATCY	3 cut(s)	4, 696, 703
PsyI	GACNNNGTC	1 cut(s)	98
PvuII	CAGCTG	1 cut(s)	1214
RsaI	GTAC	6 cut(s)	180, 202, 784, 820, 969, 1042
RsaNI	GTAC	6 cut(s)	179, 201, 783, 819, 968, 1041
RseI	CAYNNNNRTG	3 cut(s)	861, 954, 1432
SaqAI	TTAA	5 cut(s)	285, 567, 600, 1086, 1377
SatI	GCNGC	2 cut(s)	44, 990
Sau3AI	GATC	4 cut(s)	4, 696, 703, 1414
Sau96I	GGNCC	4 cut(s)	893, 1018, 1061, 1247
SchI	GAGTC	3 cut(s)	606, 724, 919
ScrFI	CCNGG	1 cut(s)	318
SduI	GDGCHC	1 cut(s)	25
SexAI	ACCWGGT	1 cut(s)	316
SfaNI	GCATC	1 cut(s)	1275
SfcI	CTRYAG	4 cut(s)	417, 887, 990, 1341
Sfr274I	CTCGAG	2 cut(s)	24, 1289
SinI	GGWCC	3 cut(s)	893, 1018, 1061
SlaI	CTCGAG	2 cut(s)	24, 1289
SmiMI	CAYNNNNRTG	3 cut(s)	861, 954, 1432
SmlI	CTYRAG	4 cut(s)	24, 284, 346, 1289
SmoI	CTYRAG	4 cut(s)	24, 284, 346, 1289
SsiI	CCGC	1 cut(s)	1277
SspI	AATATT	1 cut(s)	1006
SspMI	CTAG	3 cut(s)	69, 248, 791
StyD4I	CCNGG	1 cut(s)	316
StyI	CCWWGG	2 cut(s)	258, 1014
TaaI	ACNGT	3 cut(s)	303, 620, 787
TaiI	ACGT	3 cut(s)	76, 298, 1332
TaqI	TCGA	6 cut(s)	25, 135, 309, 1077, 1290, 1413
TatI	WGTACW	3 cut(s)	178, 782, 967
Tru1I	TTAA	5 cut(s)	285, 567, 600, 1086, 1377
Tru9I	TTAA	5 cut(s)	285, 567, 600, 1086, 1377
TscAI	CASTG	3 cut(s)	306, 625, 785
TseFI	GTSAC	3 cut(s)	365, 901, 1336
TseI	GCWGC	2 cut(s)	43, 989
Tsp45I	GTSAC	3 cut(s)	365, 901, 1336
TspDTI	ATGAA	7 cut(s)	75, 448, 458, 548, 576, 873, 1286
TspGWI	ACGGA	2 cut(s)	92, 467
TspRI	CASTG	3 cut(s)	306, 625, 785
Tth111I	GACNNNGTC	1 cut(s)	98
Vha464I	CTTAAG	1 cut(s)	284
VpaK11BI	GGWCC	3 cut(s)	893, 1018, 1061
XapI	RAATTY	3 cut(s)	563, 1196, 1304
XbaI	TCTAGA	1 cut(s)	68
XceI	RCATGY	3 cut(s)	953, 1031, 1055
XcmI	CCANNNNNNNNNTGG	1 cut(s)	211
XhoI	CTCGAG	2 cut(s)	24, 1289
XmiI	GTMKAC	2 cut(s)	886, 1166
XspI	CTAG	3 cut(s)	69, 248, 791
Zsp2I	ATGCAT	1 cut(s)	399

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.