Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0002582

Physical Location & Seq

Genomic Coordinates Reverse (-)

3010405 .. 3019649

Gene Structure 9245 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1024100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4128 bp

ATGGGTCGGAAGAAGCCGACTGCTCGTGACGACGAGAACCCTCAGCAGGCTGCGGGTGCAAAGTCGAAGAAGAAAGCTTTGGTAATTGAAGACGATGAGTATTCTATAGGAACTGAGCCGTCCGAGGAGTCTCAGGCGCAGGAAGAGAAAGTTGCTGTCACGGGAAAGAAGGGTAAAAAGGGTAATTCAAAAGCGTCAAAGCGCAGGGATGATGATGACTTTGATGATGTGGGACAGGGGGACGAGGGCGAAGATGAGGTTCCCGAAGTTGCCTTCACTGGAAAGAAGAAGGGAAAGTCGAAGAAGAGTGGTGGGAATAGTGTGTTCAGCTCTTCGACTTTTGGGCTGCTTGGAGACGAAGACGGTGATGATGATGAGAAATCTGGGTTAACTGGTGATGATGATGAGGATGCTCCAGTGGTGAGTTTTTCTGGCAAGAAGAAGGCTTCAAAATCGTCCAAGAAAACTGGTGGTAGTTTGTTTACAGGGTCAGCTTTTGATGCGATTGGTGATGAAGGCGATAGTGATGGTGAGGTTATTGATAGCTCTGTGGATAAGAGTAAAGAAGATGACGATAGTGAGAACGAAGATGAGCTTGTAATTTCTTTTACAGGGAAGAAGAAGCCGTCTAAGGGTGGGAAGAAGGGTGGGAATGTGTTTGCAGCAGCTAGCTTTGATGCCCTCGATGATGCAGACGAGGAGAAGGATGAAAAGAATGATGAGGACGATGAAGTTCCACAGATTACATTCTCCGGTAAGAAAAAGAAGTCATCAAAGGTATCAAAAAAGAGCGGTGCTAATTCTCTTAGCTCAGCTCTGCTTGATGAGGGGAATGATCAAGATACTTCTGTATCTGAACCAACTAGAGTTGGTGATGACACAGTTGATGATCAAGATGCATCTGTGATTGCATTCACAGGTAAAAAGAAGTCTTCCAAAAAGAAAGGTAATGGTGTTTTTACTGCATTGAGTGAAGATGCAGATGTATTTGAACCAGAACAACCAAACACTGAAACTAGTAAAAGCGAAGCTGATGATTCTAAAACTAACAAGAGTAAAGAGGTTCTAGAAACTTCAAAAAGTAAGAAAAAGAAGAAGAAGAGTGGACGGACTGCTCAAGAAGAAGATGACTTGGATAAGATTCTTGCTGAGCTAGGTGAGGGTCCTACTACGTTGAAACCAGCTGAGGCTACTGTGCAAGCGGAGAAAGTTGAGGTTCAACCTGATCTGGTTGCCCCGGTTGATGCTTCTGGTGAAAAGGAAGGTGAAGAAGAGACTGGGGAGTCTGCTGCCGCAAAGAAGAAGAGAAAGAAGAAGGAGAAGGAAAAGGAGAAAAAGGCAGCAGCTGCAGCCGTAGCAGGGACTGCTACTGCTCCTGTGGTCACTAAGGATGATAAACAAGAAGAAACCCAAATTGAACCTACAGATCCCAAGAAGAAAGAAGTGAAAGGTAAAGTAGCAGATAAGAAACTTCCAAAACATGTGAGGGAGATGCAAGAGGCTCTTGCTAGGCGACAGGAACTGGAAGAGAGGTTGAAAAGGGAAGCAGAGGAGAAACAGAGGAAGGAAGAAGAGGAGCGGCGTAAGCAGGAAGAACTTGAGAGGGAAAAGGAGGCGGCCAGGATTCTGAGAAGGGAAAGGGAAAAGGAGAAGCTACAAAGGAAGAGACAGGAAGGCAAGGTTTTAACGCCGAAGCAAAAAGAAGAACAACGTCGGCGGGAGGCAATGAGAAACCAGATACTTGCTAATGCAGGGGGTTTGCCTCTCCCGACCTCAGACAATGATAAAAAGGCTAAACGGCCCATGTATCAGAAAAAGAAGACAAAAGCAGCACCCATTCATGCAAATGGTGCAGCTTCTGTGAAGCCAGTGGAAAACCTAGAAGAGGGAGGCCAGCAGGACACTATCCCTGAGCCAGAGTCTGGGGAATTTGAAAAGGTTGAAGATGTAGAGTCGGTGGATCTGGAGGAAAAATCTGAAGTTGCTGAATCGGTTAAAGATAATGGAGTGGAAGAAGAAGAAGAAGAAGAAGAAGAAGATGATGATGATGATGATGATCCATGGGATGTTAAGAGCTTTGATGATGTTAACCTTTCTGTTAAACGTGGATTTTCTGATGAAGAGATTGACTCTCAACCTGTTGCAAAAAAAGACATAAAGAGTGCAGGTGCCAAGCCATCAGTTTCTGCCCAGAACACTGTTCCTTCTCAACCAATAAAATCTCAGGATGGTGAAAATAAGAAAAAACAACCTGATAATGATGTTGACAAAAGTAAGAAGAAAGATGTTTCTGTTAAAAAAGCAGCGGCAACTTCTAATGCTGACCCTAAAGAAAGTGGAGATAACCTTCGTTCACCTATTTGCTGCATAATGGGCCATGTTGATACTGGTAAAACTAAGCTGCTGGATTGTATCAGAGGCACAAATGTTCAGGAAGGTGAGGCCGGAGGTATTACTCAGCAGATTGGAGCAACATATTTTCCTGCTGAGAACATCCGTGAGAGGACCAAGGAACTGAAAGCTGATGCGAAGCTGAAGGTCCCTGGTCTGTTGGTCATTGATACCCCTGGGCACGAGTCATTCACTAATTTACGGTCACGGGGCTCAGGTTTATGTGATATTGCTATATTGGTGGTTGATATTATGCATGGCTTAGAGCCTCAGACAATAGAATCACTCAACCTTTTGAAAATGAGGAATACGGAATTCATTGTTGCGCTGAATAAGGTGGACAGACTCTACGGATGGAAAACCTGCCGCAATGCACCAATTGTGAAAGCAATGAAACAACAATCTAAGGATGTACAAAATGAGTTCAATATGAGGCTCGTACAGATTATCACTCAATTCAAGGAGCAGGGGTTAAATACTGAATTGTATTATAAGAATAAAGAAATGGGGGAAACATACAGTATTATTCCTACAAGTGCCATTAGTGGTGAAGGAATTCCAGATTTGCTATTACTATTGGTTCAGTGGACGCAGAAAACTATGGTTGAGAAACTCACTTTCAGCAATGAAATTCAGTGTACGGTTTTGGAGGTTAAGGTTATAGAAGGCCTTGGGACAACAATAGATGTTGTTTTGGTTAATGGTGTGCTTCATGAAGGAGATCAAATAGTGGTTTGCGGCATGCAGGGACCTATTGTTGCTACAATTCGGTCTCTATTGACACCACATCCAATGAAAGAACTTCGTGTGAAGGGAGCATATCTGCATCATAGTGAAATCAAGGCTGCACAGGGTATCAAAATCTCAGCACAGGGTCTTGAGCACGCTATTGCTGGTACTGCTCTACATGTGGTAGGGCCTCATGATGACTTGGACGAAATCAAGGAAACAGCTATGGAAGACATGAAGTCAGTTATGAATAGGATTGACAAGAGCGGTGAGGGAGTTTGCGTACAAGCATCCACTCTAGGTTCCTTGGAAGCATTGCTAGAGTTTTTGAAAACACCTGTCGTTAACATTCCCGTTAGTGGTATTAGCATAGGACCTGTGCATAAAAAGGATGTCATGAAGGCCAGTGTCATGCTTGAAAAGAAAAAGGAGTTTGCCACCATCTTGGCATTTGATGTCAAAGTGACACCAGAGGCCCGAGAAATGGCAGATGATTTAGGTGTGAAGATCTTCATTGCCGATATCATCTATCACTTGTTTGATCAATTTAAGGCCTATATTGAAAATATCAAGGAGGAAAAGAAGAAGGAATCTGCTGATGAAGCTGTCTTTCCTTGCGTGCTCAAGATTATGCCCAACTGTGTTTTCAACAAGAAGGATCCAATTATTTTGGGGGTTGAGGTTCTCGATGGCATTTTGAAGGTGGGTACTCCAATTTGTATTCCTCAAAGGGATTTTATTTCTATTGGGCGCATTGCATCCATTGAAAATAACCACAAACCTGTTGATACGGCGAAGAAGGGGTCGAAGTTAGCCATTAAGATTATTGGGTCAAATTCGGAAGAGCAGCAAAAGATGTTTGGTAGGCATTTTGAAATTGATGACGAACTTGTCAGCCAAATCTCAAGGAATTCGATTGATGTACTTAAAGCGAATTACCGGGATGAACTATCAATGGAGGAGTGGAAGCTGCTCGTGAAATTGAAGAAACTTTTCGAGATACCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001882 GO:0001883 GO:0002181 GO:0002183 GO:0003674 GO:0003676 GO:0003723 GO:0003743 GO:0003824 GO:0003924 GO:0005488 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006412 GO:0006413 GO:0006518 GO:0006807 GO:0008135 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019001 GO:0019538 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032991 GO:0033290 GO:0034641 GO:0034645 GO:0035639 GO:0036094 GO:0043043 GO:0043167 GO:0043168 GO:0043170 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0070993 GO:0071704 GO:0097159 GO:0097367 GO:1901265 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko00195 ko01100 ko03013 map00195 map01100 map03013

Pfam Domains

Protein Families

GTP_EFTU GTP_EFTU_D2 IF-2

Protein Analysis

1376

Amino Acids

151.07

Weight (kDa)

5.18

Isoelectric Point (pI)

37.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GTP_EFTU	PF00009	786 - 995	6.8e-35	Elongation factor Tu GTP binding domain
GTP_EFTU_D2	PF03144	1022 - 1097	2.7e-06	Elongation factor Tu domain 2
IF-2	PF11987	1112 - 1224	5.3e-22	Translation-initiation factor 2
GTP_EFTU_D4	PF14578	1245 - 1337	1.8e-15	Elongation factor Tu domain 4

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002582)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G21160 AT1G76720 AT1G76720 AT1G76810 AT2G27700 AT2G27700
fragaria_vesca	FvH4_2g13940 FvH4_2g13940 FvH4_2g13940 FvH4_4g36100 FvH4_4g36312
malus_domestica	MD05G1023200.v1.1 MD09G1087100.v1.1 MD10G1024100.v1.1
prunus_persica	Prupe.2G099300_v2.0.a1 Prupe.8G027900_v2.0.a1
pyrus_communis	pycom05g01470 pycom09g01240 pycom10g01600
rosa_chinensis	RchiOBHm_Chr4g0445721 RchiOBHm_Chr6g0275701
rosa_laevigata	RLG00000005714 RLG00000013337 RLG00000013444
rosa_multiflora	Rmu_co8469881.1_g000001 Rmu_sc0003637.1_g000011 Rmu_sc0003637.1_g000012 Rmu_sc0007122.1_g000003
rosa_roxburghii	Rroxscaffold_7G00193000
rosa_rugosa	Rorug04G0365200 Rorug06G0095700
rosa_samantha	Rh4AG426100 Rh4CG453100 Rh4DG434300 Rh6AG207600 Rh6BG210300 Rh6CG214100 Rh6DG203400
rosa_wichuraiana	Rw4G036490 Rw6G018100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2883
AarI	CACCTGC	1 cut(s)	2156
AasI	GACNNNNNNGTC	2 cut(s)	1282, 4010
Acc36I	ACCTGC	2 cut(s)	2156, 2762
AccB1I	GGYRCC	1 cut(s)	2168
AccB7I	CCANNNNNTGG	1 cut(s)	1922
AccBSI	CCGCTC	3 cut(s)	792, 1579, 3387
AclWI	GGATC	5 cut(s)	1421, 1968, 2051, 3773, 3786
AcoI	YGGCCR	1 cut(s)	1617
AcsI	RAATTY	6 cut(s)	1928, 2705, 2946, 3021, 3955, 4030
AcuI	CTGAAG	2 cut(s)	1998, 2555
AfaI	GTAC	7 cut(s)	2805, 2832, 3031, 3289, 3405, 3829, 4044
AfiI	CCNNNNNNNGG	7 cut(s)	46, 632, 1359, 1885, 1922, 3479, 3604
AflIII	ACRYGT	2 cut(s)	1480, 3298
AhdI	GACNNNNNGTC	1 cut(s)	3358
AhlI	ACTAGT	1 cut(s)	1016
AjnI	CCWGG	3 cut(s)	1619, 2542, 2566
AjuI	GAANNNNNNNTTGG	4 cut(s)	62, 94, 3749, 3781
Alw21I	GWGCWC	2 cut(s)	3276, 3744
Alw26I	GTCTC	5 cut(s)	135, 348, 1268, 1660, 3168
AlwI	GGATC	5 cut(s)	1421, 1968, 2051, 3773, 3786
AlwNI	CAGNNNCTG	6 cut(s)	1346, 1364, 1522, 1859, 1922, 2186
Ama87I	CYCGRG	1 cut(s)	3597
ApoI	RAATTY	6 cut(s)	1928, 2705, 2946, 3021, 3955, 4030
ArsI	GACNNNNNNTTYG	2 cut(s)	3908, 3940
Asp700I	GAANNNNTTC	3 cut(s)	2946, 3629, 4112
AspLEI	GCGC	4 cut(s)	139, 204, 2719, 3873
AspS9I	GGNCC	9 cut(s)	1163, 1800, 2374, 2505, 2539, 3140, 3308, 3494, 3595
AsuC2I	CCSGG	2 cut(s)	1238, 4061
AsuNHI	GCTAGC	1 cut(s)	668
AvaI	CYCGRG	1 cut(s)	3597
AvaII	GGWCC	5 cut(s)	1163, 2505, 2539, 3140, 3494
BaeGI	GKGCMC	1 cut(s)	2574
BaeI	ACNNNNGTAYC	4 cut(s)	834, 834, 867, 867
BamHI	GGATCC	1 cut(s)	3778
BanI	GGYRCC	1 cut(s)	2168
BanII	GRGCYC	1 cut(s)	2606
BauI	CACGAG	3 cut(s)	24, 2573, 4094
BbsI	GAAGAC	5 cut(s)	96, 366, 924, 1826, 3357
Bbv12I	GWGCWC	2 cut(s)	3276, 3744
BbvCI	CCTCAGC	2 cut(s)	42, 1185
BccI	CCATC	6 cut(s)	521, 2185, 2222, 2739, 3569, 3803
BceAI	ACGGC	5 cut(s)	103, 610, 1337, 1814, 3927
BciT130I	CCWGG	3 cut(s)	1621, 2544, 2568
BclI	TGATCA	3 cut(s)	835, 889, 3661
BcnI	CCSGG	2 cut(s)	1238, 4061
BcoDI	GTCTC	5 cut(s)	135, 348, 1268, 1660, 3168
BcuI	ACTAGT	1 cut(s)	1016
BfaI	CTAG	9 cut(s)	669, 864, 1017, 1067, 1154, 1509, 1880, 3419, 3440
BfmI	CTRYAG	3 cut(s)	105, 1347, 1422
BfuAI	ACCTGC	2 cut(s)	2156, 2762
BglII	AGATCT	1 cut(s)	3627
BlpI	GCTNAGC	2 cut(s)	811, 1149
Bme1390I	CCNGG	5 cut(s)	1238, 1621, 2544, 2568, 4061
Bme18I	GGWCC	5 cut(s)	1163, 2505, 2539, 3140, 3494
BmeRI	GACNNNNNGTC	1 cut(s)	3358
BmeT110I	CYCGRG	1 cut(s)	3597
BmgT120I	GGNCC	9 cut(s)	1163, 1800, 2374, 2505, 2539, 3140, 3308, 3494, 3595
BmiI	GGNNCC	7 cut(s)	261, 1164, 2170, 2541, 3141, 3424, 3780
BmrFI	CCNGG	5 cut(s)	1238, 1621, 2544, 2568, 4061
BmrI	ACTGGG	1 cut(s)	1287
BmtI	GCTAGC	1 cut(s)	672
BmuI	ACTGGG	1 cut(s)	1287
BpiI	GAAGAC	5 cut(s)	96, 366, 924, 1826, 3357
BplI	GAGNNNNNCTC	2 cut(s)	2114, 2146
BpmI	CTGGAG	2 cut(s)	399, 1985
Bpu10I	CCTNAGC	4 cut(s)	42, 1185, 1911, 2605
Bpu1102I	GCTNAGC	2 cut(s)	811, 1149
BpuEI	CTTGAG	5 cut(s)	1101, 1619, 3290, 3728, 4009
BpuMI	CCSGG	2 cut(s)	1238, 4061
BsaBI	GATNNNNATC	1 cut(s)	2055
BsaI	GGTCTC	1 cut(s)	3168
BsaJI	CCNNGG	9 cut(s)	123, 1236, 2060, 2508, 2542, 2566, 2567, 3061, 3426
BsaWI	WCCGGW	1 cut(s)	752
BsaXI	ACNNNNNCTCC	4 cut(s)	2846, 2876, 3387, 3417
Bsc4I	CCNNNNNNNGG	7 cut(s)	46, 632, 1359, 1885, 1922, 3479, 3604
Bse1I	ACTGG	9 cut(s)	283, 397, 416, 472, 1282, 1527, 1868, 2392, 3525
Bse3DI	GCAATG	7 cut(s)	1731, 2767, 2787, 3022, 3434, 3633, 3873
Bse8I	GATNNNNATC	1 cut(s)	2055
BseBI	CCWGG	3 cut(s)	1621, 2544, 2568
BseDI	CCNNGG	9 cut(s)	123, 1236, 2060, 2508, 2542, 2566, 2567, 3061, 3426
BseJI	GATNNNNATC	1 cut(s)	2055
BseLI	CCNNNNNNNGG	7 cut(s)	46, 632, 1359, 1885, 1922, 3479, 3604
BseMI	GCAATG	7 cut(s)	1731, 2767, 2787, 3022, 3434, 3633, 3873
BseNI	ACTGG	9 cut(s)	283, 397, 416, 472, 1282, 1527, 1868, 2392, 3525
BseRI	GAGGAG	5 cut(s)	140, 713, 1565, 1589, 4094
BseSI	GKGCMC	1 cut(s)	2574
BsgI	GTGCAG	3 cut(s)	1872, 2184, 3222
BshNI	GGYRCC	1 cut(s)	2168
BsiHKAI	GWGCWC	2 cut(s)	3276, 3744
BsiHKCI	CYCGRG	1 cut(s)	3597
BsiSI	CCGG	4 cut(s)	753, 1238, 2445, 4060
BslFI	GGGAC	6 cut(s)	246, 254, 1375, 2525, 3079, 3153
BslI	CCNNNNNNNGG	7 cut(s)	46, 632, 1359, 1885, 1922, 3479, 3604
BsmAI	GTCTC	5 cut(s)	135, 348, 1268, 1660, 3168
BsmBI	CGTCTC	1 cut(s)	348
BsmFI	GGGAC	6 cut(s)	246, 254, 1375, 2525, 3079, 3153
BsmI	GAATGC	1 cut(s)	911
Bso31I	GGTCTC	1 cut(s)	3168
BsoBI	CYCGRG	1 cut(s)	3597
Bsp1286I	GDGCHC	4 cut(s)	2574, 2606, 3276, 3744
Bsp1407I	TGTACA	1 cut(s)	2803
Bsp1720I	GCTNAGC	2 cut(s)	811, 1149
Bsp19I	CCATGG	1 cut(s)	2060
BspHI	TCATGA	3 cut(s)	3103, 3313, 3516
BspLI	GGNNCC	7 cut(s)	261, 1164, 2170, 2541, 3141, 3424, 3780
BspMAI	CTGCAG	1 cut(s)	1351
BspMI	ACCTGC	2 cut(s)	2156, 2762
BspOI	GCTAGC	1 cut(s)	672
BspPI	GGATC	5 cut(s)	1421, 1968, 2051, 3773, 3786
BspQI	GCTCTTC	2 cut(s)	337, 3957
BspT107I	GGYRCC	1 cut(s)	2168
BspTNI	GGTCTC	1 cut(s)	3168
BsrBI	CCGCTC	3 cut(s)	792, 1579, 3387
BsrDI	GCAATG	7 cut(s)	1731, 2767, 2787, 3022, 3434, 3633, 3873
BsrGI	TGTACA	1 cut(s)	2803
BsrI	ACTGG	9 cut(s)	283, 397, 416, 472, 1282, 1527, 1868, 2392, 3525
BssECI	CCNNGG	9 cut(s)	123, 1236, 2060, 2508, 2542, 2566, 2567, 3061, 3426
BssSI	CACGAG	3 cut(s)	24, 2573, 4094
BssT1I	CCWWGG	4 cut(s)	2060, 2508, 3061, 3426
Bst2BI	CACGAG	3 cut(s)	24, 2573, 4094
Bst2UI	CCWGG	3 cut(s)	1621, 2544, 2568
Bst4CI	ACNGT	8 cut(s)	365, 883, 1195, 2200, 2595, 2912, 3034, 3761
BstAPI	GCANNNNNTGC	3 cut(s)	1346, 1364, 1850
BstAUI	TGTACA	1 cut(s)	2803
BstC8I	GCNNGC	7 cut(s)	48, 670, 1200, 1895, 3134, 3276, 3740
BstDSI	CCRYGG	1 cut(s)	2060
BstENI	CCTNNNNNAGG	1 cut(s)	1883
BstHHI	GCGC	4 cut(s)	139, 204, 2719, 3873
BstMAI	GTCTC	5 cut(s)	135, 348, 1268, 1660, 3168
BstNI	CCWGG	3 cut(s)	1621, 2544, 2568
BstNSI	RCATGY	3 cut(s)	1484, 3136, 3302
BstSCI	CCNGG	5 cut(s)	1236, 1619, 2542, 2566, 4059
BstSFI	CTRYAG	3 cut(s)	105, 1347, 1422
BstSLI	GKGCMC	1 cut(s)	2574
BstV2I	GAAGAC	5 cut(s)	96, 366, 924, 1826, 3357
BstX2I	RGATCY	4 cut(s)	1426, 1960, 3627, 3778
BstXI	CCANNNNNNTGG	1 cut(s)	3565
BstYI	RGATCY	4 cut(s)	1426, 1960, 3627, 3778
BtgI	CCRYGG	1 cut(s)	2060
BtsIMutI	CAGTG	8 cut(s)	276, 423, 1008, 1875, 2196, 2981, 3032, 3532
BveI	ACCTGC	2 cut(s)	2156, 2762
Cac8I	GCNNGC	7 cut(s)	48, 670, 1200, 1895, 3134, 3276, 3740
CaiI	CAGNNNCTG	6 cut(s)	1346, 1364, 1522, 1859, 1922, 2186
CciI	TCATGA	3 cut(s)	3103, 3313, 3516
CfoI	GCGC	4 cut(s)	139, 204, 2719, 3873
Cfr13I	GGNCC	9 cut(s)	1163, 1800, 2374, 2505, 2539, 3140, 3308, 3494, 3595
CseI	GACGC	2 cut(s)	183, 2989
Csp6I	GTAC	7 cut(s)	2804, 2831, 3030, 3288, 3404, 3828, 4043
CviQI	GTAC	7 cut(s)	2804, 2831, 3030, 3288, 3404, 3828, 4043
DrdI	GACNNNNNNGTC	2 cut(s)	1282, 4010
DriI	GACNNNNNGTC	1 cut(s)	3358
DseDI	GACNNNNNNGTC	2 cut(s)	1282, 4010
EaeI	YGGCCR	1 cut(s)	1617
Eam1105I	GACNNNNNGTC	1 cut(s)	3358
Eco130I	CCWWGG	4 cut(s)	2060, 2508, 3061, 3426
Eco147I	AGGCCT	2 cut(s)	3060, 3674
Eco24I	GRGCYC	1 cut(s)	2606
Eco31I	GGTCTC	1 cut(s)	3168
Eco32I	GATATC	1 cut(s)	3643
Eco47I	GGWCC	5 cut(s)	1163, 2505, 2539, 3140, 3494
Eco57I	CTGAAG	2 cut(s)	1998, 2555
Eco88I	CYCGRG	1 cut(s)	3597
EcoNI	CCTNNNNNAGG	1 cut(s)	1883
EcoO109I	RGGNCCY	5 cut(s)	1163, 2539, 3140, 3308, 3494
EcoRI	GAATTC	3 cut(s)	2705, 2946, 4030
EcoRII	CCWGG	3 cut(s)	1619, 2542, 2566
EcoRV	GATATC	1 cut(s)	3643
EcoT14I	CCWWGG	4 cut(s)	2060, 2508, 3061, 3426
EcoT22I	ATGCAT	2 cut(s)	901, 2649
EcoT38I	GRGCYC	1 cut(s)	2606
ErhI	CCWWGG	4 cut(s)	2060, 2508, 3061, 3426
Esp3I	CGTCTC	1 cut(s)	348
FalI	AAGNNNNNCTT	4 cut(s)	579, 611, 1488, 1520
FaqI	GGGAC	6 cut(s)	246, 254, 1375, 2525, 3079, 3153
FauI	CCCGC	2 cut(s)	46, 1710
FbaI	TGATCA	3 cut(s)	835, 889, 3661
FriOI	GRGCYC	1 cut(s)	2606
FspBI	CTAG	9 cut(s)	669, 864, 1017, 1067, 1154, 1509, 1880, 3419, 3440
GlaI	GCGC	4 cut(s)	138, 203, 2718, 3872
GsuI	CTGGAG	2 cut(s)	399, 1985
HapII	CCGG	4 cut(s)	753, 1238, 2445, 4060
HgaI	GACGC	2 cut(s)	183, 2989
HhaI	GCGC	4 cut(s)	139, 204, 2719, 3873
Hin6I	GCGC	4 cut(s)	137, 202, 2717, 3871
HinP1I	GCGC	4 cut(s)	137, 202, 2717, 3871
HincII	GTYRAC	4 cut(s)	390, 2089, 2266, 3466
HindII	GTYRAC	4 cut(s)	390, 2089, 2266, 3466
HindIII	AAGCTT	1 cut(s)	75
HpaI	GTTAAC	3 cut(s)	390, 2089, 3466
HpaII	CCGG	4 cut(s)	753, 1238, 2445, 4060
Hpy99I	CGWCG	2 cut(s)	35, 1716
HpyCH4III	ACNGT	8 cut(s)	365, 883, 1195, 2200, 2595, 2912, 3034, 3761
HpyCH4IV	ACGT	3 cut(s)	1172, 1711, 2104
HpySE526I	ACGT	3 cut(s)	1172, 1711, 2104
HspAI	GCGC	4 cut(s)	137, 202, 2717, 3871
Ksp22I	TGATCA	3 cut(s)	835, 889, 3661
KspAI	GTTAAC	3 cut(s)	390, 2089, 3466
LguI	GCTCTTC	2 cut(s)	337, 3957
LmnI	GCTCC	6 cut(s)	418, 1378, 1576, 2468, 2854, 3206
MaeI	CTAG	9 cut(s)	669, 864, 1017, 1067, 1154, 1509, 1880, 3419, 3440
MaeII	ACGT	3 cut(s)	1172, 1711, 2104
MaeIII	GTNAC	5 cut(s)	26, 157, 1381, 2595, 3583
MbiI	CCGCTC	3 cut(s)	792, 1579, 3387
MfeI	CAATTG	1 cut(s)	2769
MflI	RGATCY	4 cut(s)	1426, 1960, 3627, 3778
MhlI	GDGCHC	4 cut(s)	2574, 2606, 3276, 3744
MlyI	GAGTC	7 cut(s)	137, 1292, 1928, 1961, 2123, 2585, 2730
Mph1103I	ATGCAT	2 cut(s)	901, 2649
MroXI	GAANNNNTTC	3 cut(s)	2946, 3629, 4112
MslI	CAYNNNNRTG	2 cut(s)	1845, 3222
MspA1I	CMGCKG	3 cut(s)	1184, 1346, 2306
MspI	CCGG	4 cut(s)	753, 1238, 2445, 4060
MspR9I	CCNGG	5 cut(s)	1238, 1621, 2544, 2568, 4061
MunI	CAATTG	1 cut(s)	2769
Mva1269I	GAATGC	1 cut(s)	911
MvaI	CCWGG	3 cut(s)	1621, 2544, 2568
NciI	CCSGG	2 cut(s)	1238, 4061
NcoI	CCATGG	1 cut(s)	2060
NheI	GCTAGC	1 cut(s)	668
NlaIV	GGNNCC	7 cut(s)	261, 1164, 2170, 2541, 3141, 3424, 3780
NmuCI	GTSAC	5 cut(s)	26, 157, 1381, 2595, 3583
NsiI	ATGCAT	2 cut(s)	901, 2649
NspI	RCATGY	3 cut(s)	1484, 3136, 3302
PaeI	GCATGC	1 cut(s)	3136
PagI	TCATGA	3 cut(s)	3103, 3313, 3516
PaqCI	CACCTGC	1 cut(s)	2156
PasI	CCCWGGG	1 cut(s)	2567
PceI	AGGCCT	2 cut(s)	3060, 3674
PciI	ACATGT	2 cut(s)	1480, 3298
PciSI	GCTCTTC	2 cut(s)	337, 3957
PctI	GAATGC	1 cut(s)	911
PdmI	GAANNNNTTC	3 cut(s)	2946, 3629, 4112
PfeI	GAWTC	6 cut(s)	1037, 1141, 1624, 1988, 2672, 3710
PflMI	CCANNNNNTGG	1 cut(s)	1922
PleI	GAGTC	7 cut(s)	136, 1291, 1927, 1960, 2123, 2584, 2730
PpsI	GAGTC	7 cut(s)	136, 1291, 1927, 1960, 2123, 2584, 2730
PpuMI	RGGWCCY	4 cut(s)	1163, 2539, 3140, 3494
PscI	ACATGT	2 cut(s)	1480, 3298
PsiI	TTATAA	1 cut(s)	2883
Psp5II	RGGWCCY	4 cut(s)	1163, 2539, 3140, 3494
Psp6I	CCWGG	3 cut(s)	1619, 2542, 2566
PspGI	CCWGG	3 cut(s)	1619, 2542, 2566
PspN4I	GGNNCC	7 cut(s)	261, 1164, 2170, 2541, 3141, 3424, 3780
PspPI	GGNCC	9 cut(s)	1163, 1800, 2374, 2505, 2539, 3140, 3308, 3494, 3595
PspPPI	RGGWCCY	4 cut(s)	1163, 2539, 3140, 3494
PstI	CTGCAG	1 cut(s)	1351
PstNI	CAGNNNCTG	6 cut(s)	1346, 1364, 1522, 1859, 1922, 2186
PsuI	RGATCY	4 cut(s)	1426, 1960, 3627, 3778
PvuII	CAGCTG	2 cut(s)	1184, 1346
RsaI	GTAC	7 cut(s)	2805, 2832, 3031, 3289, 3405, 3829, 4044
RsaNI	GTAC	7 cut(s)	2804, 2831, 3030, 3288, 3404, 3828, 4043
RseI	CAYNNNNRTG	2 cut(s)	1845, 3222
SapI	GCTCTTC	2 cut(s)	337, 3957
Sau96I	GGNCC	9 cut(s)	1163, 1800, 2374, 2505, 2539, 3140, 3308, 3494, 3595
SchI	GAGTC	7 cut(s)	137, 1292, 1928, 1961, 2123, 2585, 2730
ScrFI	CCNGG	5 cut(s)	1238, 1621, 2544, 2568, 4061
SduI	GDGCHC	4 cut(s)	2574, 2606, 3276, 3744
SfcI	CTRYAG	3 cut(s)	105, 1347, 1422
SinI	GGWCC	5 cut(s)	1163, 2505, 2539, 3140, 3494
SmiMI	CAYNNNNRTG	2 cut(s)	1845, 3222
SmlI	CTYRAG	5 cut(s)	1116, 1598, 3269, 3743, 4024
SmoI	CTYRAG	5 cut(s)	1116, 1598, 3269, 3743, 4024
SpeI	ACTAGT	1 cut(s)	1016
SphI	GCATGC	1 cut(s)	3136
SseBI	AGGCCT	2 cut(s)	3060, 3674
SspMI	CTAG	9 cut(s)	669, 864, 1017, 1067, 1154, 1509, 1880, 3419, 3440
StuI	AGGCCT	2 cut(s)	3060, 3674
StyD4I	CCNGG	5 cut(s)	1236, 1619, 2542, 2566, 4059
StyI	CCWWGG	4 cut(s)	2060, 2508, 3061, 3426
TaaI	ACNGT	8 cut(s)	365, 883, 1195, 2200, 2595, 2912, 3034, 3761
TaiI	ACGT	3 cut(s)	1175, 1714, 2107
TaqI	TCGA	8 cut(s)	65, 299, 335, 684, 3807, 3926, 4034, 4116
TaqII	GACCGA	1 cut(s)	3150
TatI	WGTACW	2 cut(s)	2803, 4042
TauI	GCSGC	6 cut(s)	1295, 1582, 1619, 2309, 2760, 3132
TfiI	GAWTC	6 cut(s)	1037, 1141, 1624, 1988, 2672, 3710
TscAI	CASTG	8 cut(s)	283, 423, 1015, 1875, 2203, 2981, 3032, 3532
TseFI	GTSAC	5 cut(s)	26, 157, 1381, 2595, 3583
Tsp45I	GTSAC	5 cut(s)	26, 157, 1381, 2595, 3583
TspGWI	ACGGA	4 cut(s)	1123, 2486, 2717, 2757
TspRI	CASTG	8 cut(s)	283, 423, 1015, 1875, 2203, 2981, 3032, 3532
Van91I	CCANNNNNTGG	1 cut(s)	1922
VpaK11BI	GGWCC	5 cut(s)	1163, 2505, 2539, 3140, 3494
XagI	CCTNNNNNAGG	1 cut(s)	1883
XapI	RAATTY	6 cut(s)	1928, 2705, 2946, 3021, 3955, 4030
XbaI	TCTAGA	1 cut(s)	1066
XceI	RCATGY	3 cut(s)	1484, 3136, 3302
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2384
XmnI	GAANNNNTTC	3 cut(s)	2946, 3629, 4112
XspI	CTAG	9 cut(s)	669, 864, 1017, 1067, 1154, 1509, 1880, 3419, 3440
Zsp2I	ATGCAT	2 cut(s)	901, 2649

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.