Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0002582

Physical Location & Seq

Genomic Coordinates Forward (+)

63313862 .. 63315643

Gene Structure 1782 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G036490.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1782 bp

ATGGGGCATGTTGATGCTGGTAAAACCAAGTTGCTGGATTGCATCCGCGGCACGAATGTTCAGAAAGGTGAGGCTGGTGGTATTACTCAGCAGATTGGTGCAACTTATCTTCCTGCTGAGAATATACAAGAGAGAATCAAGGAGCTGAAAGCTGCTGATGATAAGCACATGAAGGTACCGGGTCTGTTGCTTATTGATACCCCAGGGCATGAGCCATTCACTAAGTTACGGTCACGGGGTTCTGGTTTGTGTGACATTGCTGTTTTGGTTGTTGACATTATGGATGGCTTAAAGCCTCAGACTATAGAGTCACTCAATCTTTTGAAGTCAAGGAATACTGAATTCATTGTTGCATTGAATAAGGTGGACAGACTCAACGGGTGGAAGCCTTGTCCAAATGTACCAATTATCAAGGCAATGAAGCAGCAATCTCAGGATGTTAAAAATGAGTTCAATATGAGAGTCGTGCAGATTGCCACTCAGTTCAAGGAGCAGGGGATTAATACTAAATTGAACGTCGATGAGAACAAACGGATGAGGGAAACATACAGCATTGTACCTATAAGTGCCAAGAGTGGTGAAGGCATCCCAGATTTGTTGTCACTGATGGTTTATTGGACCCAGAAATCTATGACAGAGAAACTGACTTACAGCAATGAAGTGCAGTGTACAGTGTTGGAGGTCAAGTATGTTGAAGGCCTTGGGACAACAATTGATGTTATATTGGTTAATGGGGTGCTTCGCCGTGGAGATCAAATAGTTTTTTGTGGTAACCATGGTACTCCTATTGTTACTAGGATTCAATCTTTGTTGACCCCAGAGCCGATGAAAGAGCTTCGTGTGAAGGGAAAGTATCAGCATCATAGTGAAATCAGAGCTGCGCAGGCTATCAAGATCACATCACCACAGGGGTTTGAACATGCTATTGCTGGCACTGCTCTCTATGTAGTAGGGCCTGACGATGACTTGGAAGAGGTCAAGGAAGCAGCTATGGAAGACATGATGAAGTCAGTCCTTGTGAACAACAACAAGAATGGTAGTACTAGTACTGCTGGTGACGGAGGAGTTTGTGCACAAGCATCAACTCTAGGCTCTTTGGAAGCAATGCTAGAGTTTCTGAAGGCCCAAGAAGTTAATATTCCAGTTCGTGGAATTGGCATAGGCCCTATACAGAAAAAGGATGTCAGGAAGGCCAGTGTGATGCTCGGAAAGAAGATCTTGGCATTTGATGTCAAAGTGACACCGGAGGCCCAGAAATTTGCAGATGAATTGGGTGTGAAGATTTTTGTTGGTGATATAATCCATGAGGTGATTAAGGCCTACATGAAAAACCTCAAGGAGGAAAAGGAGAAGGAGTTGGCTGCTGTGGATGAGGAAGCTGTCTTTCCATGTGTGCTCAAGATTTTACCCAACTGTGTTTTCAACAAGAAGGATCCAATCATAGTTGGGGTTAAAGTTCTTCAAGGCATTGTCAAGGTTGGGACTCCACTTTGTATTCCTCAGAGGGACTTTGTGGTTATTGGGCGCATTGAGTCGATTGAGAATAACCATAAACGTGTTGATATGGCCAACAAGGGGAAGGAAGTTTGCATTAAGATTGTTGGGACGAATCCTAATCAGCAGCAGGTGATGTTTGGTAGGCACTTTAACATTGAAGATGAACTTGTCAGCCATATCTCAAGGAGGTCTATTGATGTGCTAAAATCATGCTACCGGAACGACCTGTCGCAGGTGGAGTGGAAGCTGGTTTTGAAATTGAAGACTATTTTCAAGATACTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001882 GO:0001883 GO:0002181 GO:0002183 GO:0003674 GO:0003676 GO:0003723 GO:0003743 GO:0003824 GO:0003924 GO:0005488 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006412 GO:0006413 GO:0006518 GO:0006807 GO:0008135 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019001 GO:0019538 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032991 GO:0033290 GO:0034641 GO:0034645 GO:0035639 GO:0036094 GO:0043043 GO:0043167 GO:0043168 GO:0043170 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0070993 GO:0071704 GO:0097159 GO:0097367 GO:1901265 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko00195 ko01100 ko03013 map00195 map01100 map03013

Pfam Domains

Protein Families

GTP_EFTU GTP_EFTU_D2 IF-2

Protein Analysis

593

Amino Acids

65.72

Weight (kDa)

8.72

Isoelectric Point (pI)

26.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GTP_EFTU	PF00009	1 - 207	1.5e-32	Elongation factor Tu GTP binding domain
GTP_EFTU_D2	PF03144	235 - 280	3.8e-07	Elongation factor Tu domain 2
IF-2	PF11987	350 - 440	1.2e-16	Translation-initiation factor 2
GTP_EFTU_D4	PF14578	463 - 555	1.1e-17	Elongation factor Tu domain 4

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002582)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G21160 AT1G76720 AT1G76720 AT1G76810 AT2G27700 AT2G27700
fragaria_vesca	FvH4_2g13940 FvH4_2g13940 FvH4_2g13940 FvH4_4g36100 FvH4_4g36312
malus_domestica	MD05G1023200.v1.1 MD09G1087100.v1.1 MD10G1024100.v1.1
prunus_persica	Prupe.2G099300_v2.0.a1 Prupe.8G027900_v2.0.a1
pyrus_communis	pycom05g01470 pycom09g01240 pycom10g01600
rosa_chinensis	RchiOBHm_Chr4g0445721 RchiOBHm_Chr6g0275701
rosa_laevigata	RLG00000005714 RLG00000013337 RLG00000013444
rosa_multiflora	Rmu_co8469881.1_g000001 Rmu_sc0003637.1_g000011 Rmu_sc0003637.1_g000012 Rmu_sc0007122.1_g000003
rosa_roxburghii	Rroxscaffold_7G00193000
rosa_rugosa	Rorug04G0365200 Rorug06G0095700
rosa_samantha	Rh4AG426100 Rh4CG453100 Rh4DG434300 Rh6AG207600 Rh6BG210300 Rh6CG214100 Rh6DG203400
rosa_wichuraiana	Rw4G036490 Rw6G018100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	1615, 1720
AasI	GACNNNNNNGTC	1 cut(s)	307
Acc16I	TGCGCA	1 cut(s)	882
Acc36I	ACCTGC	2 cut(s)	1615, 1720
Acc65I	GGTACC	1 cut(s)	175
AccB1I	GGYRCC	1 cut(s)	175
AccB7I	CCANNNNNTGG	1 cut(s)	1148
AccII	CGCG	1 cut(s)	48
AciI	CCGC	2 cut(s)	46, 48
AclWI	GGATC	2 cut(s)	1427, 1440
AcoI	YGGCCR	1 cut(s)	1566
AcsI	RAATTY	2 cut(s)	341, 1256
AcuI	CTGAAG	1 cut(s)	1139
AfaI	GTAC	7 cut(s)	177, 402, 558, 670, 781, 1042, 1048
AfiI	CCNNNNNNNGG	4 cut(s)	1148, 1339, 1575, 1729
AflIII	ACRYGT	1 cut(s)	1555
AhlI	ACTAGT	1 cut(s)	1043
AjnI	CCWGG	1 cut(s)	202
AjuI	GAANNNNNNNTTGG	2 cut(s)	1403, 1435
AluBI	AGCT	7 cut(s)	145, 152, 835, 878, 989, 1379, 1744
AluI	AGCT	7 cut(s)	145, 152, 835, 878, 989, 1379, 1744
Alw21I	GWGCWC	2 cut(s)	1075, 1398
Alw44I	GTGCAC	1 cut(s)	1071
AlwI	GGATC	2 cut(s)	1427, 1440
AoxI	GGCC	8 cut(s)	697, 953, 1122, 1162, 1191, 1248, 1317, 1566
ApaLI	GTGCAC	1 cut(s)	1071
ApeKI	GCWGC	6 cut(s)	152, 424, 878, 986, 1361, 1621
ApoI	RAATTY	2 cut(s)	341, 1256
AseI	ATTAAT	1 cut(s)	501
Asp718I	GGTACC	1 cut(s)	175
AspLEI	GCGC	2 cut(s)	883, 1527
AspS9I	GGNCC	5 cut(s)	618, 953, 1123, 1163, 1249
AsuC2I	CCSGG	1 cut(s)	180
AsuHPI	GGTGA	7 cut(s)	80, 590, 894, 1067, 1304, 1321, 1639
AvaII	GGWCC	1 cut(s)	618
BaeGI	GKGCMC	1 cut(s)	1075
BalI	TGGCCA	1 cut(s)	1568
BamHI	GGATCC	1 cut(s)	1432
BanI	GGYRCC	1 cut(s)	175
BbsI	GAAGAC	2 cut(s)	1002, 1766
Bbv12I	GWGCWC	2 cut(s)	1075, 1398
BbvI	GCAGC	6 cut(s)	139, 436, 865, 998, 1348, 1633
BccI	CCATC	2 cut(s)	278, 601
BceAI	ACGGC	1 cut(s)	729
BciT130I	CCWGG	1 cut(s)	204
BcnI	CCSGG	1 cut(s)	180
BcuI	ACTAGT	1 cut(s)	1043
BfaI	CTAG	4 cut(s)	795, 1044, 1088, 1109
BfmI	CTRYAG	1 cut(s)	303
BfuAI	ACCTGC	2 cut(s)	1615, 1720
BglII	AGATCT	1 cut(s)	1215
BisI	GCNGC	7 cut(s)	49, 153, 425, 879, 987, 1362, 1622
BlsI	GCNGC	7 cut(s)	50, 154, 426, 880, 988, 1363, 1623
BmcAI	AGTACT	2 cut(s)	1042, 1048
Bme1390I	CCNGG	2 cut(s)	180, 204
Bme18I	GGWCC	1 cut(s)	618
BmgT120I	GGNCC	5 cut(s)	618, 953, 1123, 1163, 1249
BmiI	GGNNCC	3 cut(s)	177, 620, 1434
BmrFI	CCNGG	2 cut(s)	180, 204
BmsI	GCATC	6 cut(s)	4, 51, 594, 868, 1088, 1191
BpiI	GAAGAC	2 cut(s)	1002, 1766
BpuEI	CTTGAG	3 cut(s)	1319, 1382, 1663
BpuMI	CCSGG	1 cut(s)	180
BsaJI	CCNNGG	6 cut(s)	46, 202, 203, 700, 745, 775
BsaWI	WCCGGW	2 cut(s)	1243, 1713
BsaXI	ACNNNNNCTCC	4 cut(s)	671, 701, 1340, 1370
Bsc4I	CCNNNNNNNGG	4 cut(s)	1148, 1339, 1575, 1729
Bse1I	ACTGG	2 cut(s)	1142, 1194
Bse3DI	GCAATG	4 cut(s)	255, 423, 661, 1110
BseBI	CCWGG	1 cut(s)	204
BseDI	CCNNGG	6 cut(s)	46, 202, 203, 700, 745, 775
BseGI	GGATG	7 cut(s)	42, 289, 442, 540, 585, 1186, 1375
BseLI	CCNNNNNNNGG	4 cut(s)	1148, 1339, 1575, 1729
BseMI	GCAATG	4 cut(s)	255, 423, 661, 1110
BseMII	CTCAG	6 cut(s)	101, 108, 311, 446, 494, 1514
BseNI	ACTGG	2 cut(s)	1142, 1194
BseRI	GAGGAG	1 cut(s)	1077
BseSI	GKGCMC	1 cut(s)	1075
BseXI	GCAGC	6 cut(s)	139, 436, 865, 998, 1348, 1633
BsgI	GTGCAG	2 cut(s)	488, 683
Bsh1236I	CGCG	1 cut(s)	48
BshFI	GGCC	8 cut(s)	699, 955, 1124, 1164, 1193, 1250, 1319, 1568
BshNI	GGYRCC	1 cut(s)	175
BsiHKAI	GWGCWC	2 cut(s)	1075, 1398
BsiSI	CCGG	3 cut(s)	179, 1244, 1714
BslFI	GGGAC	4 cut(s)	718, 1495, 1520, 1618
BslI	CCNNNNNNNGG	4 cut(s)	1148, 1339, 1575, 1729
BsmFI	GGGAC	4 cut(s)	718, 1495, 1520, 1618
BsnI	GGCC	8 cut(s)	699, 955, 1124, 1164, 1193, 1250, 1319, 1568
Bsp1286I	GDGCHC	2 cut(s)	1075, 1398
Bsp1407I	TGTACA	1 cut(s)	668
Bsp143I	GATC	4 cut(s)	751, 894, 1215, 1432
Bsp19I	CCATGG	1 cut(s)	775
BspACI	CCGC	2 cut(s)	46, 48
BspANI	GGCC	8 cut(s)	699, 955, 1124, 1164, 1193, 1250, 1319, 1568
BspCNI	CTCAG	6 cut(s)	100, 109, 310, 445, 493, 1513
BspFNI	CGCG	1 cut(s)	48
BspLI	GGNNCC	3 cut(s)	177, 620, 1434
BspMI	ACCTGC	2 cut(s)	1615, 1720
BspPI	GGATC	2 cut(s)	1427, 1440
BspT107I	GGYRCC	1 cut(s)	175
BsrDI	GCAATG	4 cut(s)	255, 423, 661, 1110
BsrGI	TGTACA	1 cut(s)	668
BsrI	ACTGG	2 cut(s)	1142, 1194
BssECI	CCNNGG	6 cut(s)	46, 202, 203, 700, 745, 775
BssMI	GATC	4 cut(s)	751, 894, 1215, 1432
BssT1I	CCWWGG	2 cut(s)	700, 775
Bst2UI	CCWGG	1 cut(s)	204
Bst4CI	ACNGT	4 cut(s)	231, 673, 1415, 1779
Bst6I	CTCTTC	1 cut(s)	966
BstAUI	TGTACA	1 cut(s)	668
BstC8I	GCNNGC	2 cut(s)	885, 931
BstDEI	CTNAG	7 cut(s)	87, 117, 222, 297, 432, 480, 1500
BstDSI	CCRYGG	3 cut(s)	46, 745, 775
BstEII	GGTNACC	1 cut(s)	770
BstENI	CCTNNNNNAGG	2 cut(s)	1337, 1727
BstF5I	GGATG	7 cut(s)	42, 289, 442, 540, 585, 1186, 1375
BstFNI	CGCG	1 cut(s)	48
BstHHI	GCGC	2 cut(s)	883, 1527
BstKTI	GATC	4 cut(s)	754, 897, 1218, 1435
BstMBI	GATC	4 cut(s)	751, 894, 1215, 1432
BstMWI	GCNNNNNNNGC	3 cut(s)	48, 884, 935
BstNI	CCWGG	1 cut(s)	204
BstNSI	RCATGY	2 cut(s)	11, 923
BstPI	GGTNACC	1 cut(s)	770
BstSCI	CCNGG	2 cut(s)	178, 202
BstSFI	CTRYAG	1 cut(s)	303
BstSLI	GKGCMC	1 cut(s)	1075
BstUI	CGCG	1 cut(s)	48
BstV1I	GCAGC	6 cut(s)	139, 436, 865, 998, 1348, 1633
BstV2I	GAAGAC	2 cut(s)	1002, 1766
BstX2I	RGATCY	2 cut(s)	1215, 1432
BstXI	CCANNNNNNTGG	1 cut(s)	34
BstYI	RGATCY	2 cut(s)	1215, 1432
BsuRI	GGCC	8 cut(s)	699, 955, 1124, 1164, 1193, 1250, 1319, 1568
BtgI	CCRYGG	3 cut(s)	46, 745, 775
BtsCI	GGATG	7 cut(s)	42, 289, 442, 540, 585, 1186, 1375
BtsI	GCAGTG	2 cut(s)	671, 933
BtsIMutI	CAGTG	5 cut(s)	602, 671, 678, 933, 1201
BveI	ACCTGC	2 cut(s)	1615, 1720
Cac8I	GCNNGC	2 cut(s)	885, 931
CfoI	GCGC	2 cut(s)	883, 1527
Cfr13I	GGNCC	5 cut(s)	618, 953, 1123, 1163, 1249
Cfr42I	CCGCGG	1 cut(s)	49
Csp6I	GTAC	7 cut(s)	176, 401, 557, 669, 780, 1041, 1047
CviQI	GTAC	7 cut(s)	176, 401, 557, 669, 780, 1041, 1047
DdeI	CTNAG	7 cut(s)	87, 117, 222, 297, 432, 480, 1500
DpnI	GATC	4 cut(s)	753, 896, 1217, 1434
DpnII	GATC	4 cut(s)	751, 894, 1215, 1432
DrdI	GACNNNNNNGTC	1 cut(s)	307
DseDI	GACNNNNNNGTC	1 cut(s)	307
EaeI	YGGCCR	1 cut(s)	1566
Eam1104I	CTCTTC	1 cut(s)	966
EarI	CTCTTC	1 cut(s)	966
Eco130I	CCWWGG	2 cut(s)	700, 775
Eco147I	AGGCCT	2 cut(s)	699, 1319
Eco47I	GGWCC	1 cut(s)	618
Eco57I	CTGAAG	1 cut(s)	1139
Eco91I	GGTNACC	1 cut(s)	770
EcoNI	CCTNNNNNAGG	2 cut(s)	1337, 1727
EcoO109I	RGGNCCY	2 cut(s)	953, 1163
EcoO65I	GGTNACC	1 cut(s)	770
EcoRI	GAATTC	1 cut(s)	341
EcoRII	CCWGG	1 cut(s)	202
EcoT14I	CCWWGG	2 cut(s)	700, 775
ErhI	CCWWGG	2 cut(s)	700, 775
FalI	AAGNNNNNCTT	4 cut(s)	1202, 1234, 1647, 1679
FaqI	GGGAC	4 cut(s)	718, 1495, 1520, 1618
Fnu4HI	GCNGC	7 cut(s)	49, 153, 425, 879, 987, 1362, 1622
FokI	GGATG	7 cut(s)	29, 296, 449, 547, 572, 1193, 1382
Fsp4HI	GCNGC	7 cut(s)	49, 153, 425, 879, 987, 1362, 1622
FspBI	CTAG	4 cut(s)	795, 1044, 1088, 1109
FspI	TGCGCA	1 cut(s)	882
GlaI	GCGC	2 cut(s)	882, 1526
GluI	GCNGC	7 cut(s)	49, 153, 425, 879, 987, 1362, 1622
HaeIII	GGCC	8 cut(s)	699, 955, 1124, 1164, 1193, 1250, 1319, 1568
HapII	CCGG	3 cut(s)	179, 1244, 1714
HhaI	GCGC	2 cut(s)	883, 1527
Hin6I	GCGC	2 cut(s)	881, 1525
HinP1I	GCGC	2 cut(s)	881, 1525
HincII	GTYRAC	2 cut(s)	274, 813
HindII	GTYRAC	2 cut(s)	274, 813
HinfI	GANTC	8 cut(s)	135, 308, 372, 462, 799, 1483, 1532, 1609
HpaII	CCGG	3 cut(s)	179, 1244, 1714
HphI	GGTGA	7 cut(s)	80, 590, 894, 1067, 1304, 1321, 1639
Hpy166II	GTNNAC	6 cut(s)	274, 367, 669, 813, 1021, 1073
Hpy188I	TCNGA	6 cut(s)	63, 300, 875, 1119, 1208, 1503
Hpy188III	TCNNGA	5 cut(s)	434, 892, 1186, 1399, 1771
Hpy8I	GTNNAC	6 cut(s)	274, 367, 669, 813, 1021, 1073
Hpy99I	CGWCG	1 cut(s)	521
HpyAV	CCTTC	9 cut(s)	166, 575, 689, 838, 1114, 1183, 1345, 1423, 1573
HpyCH4III	ACNGT	4 cut(s)	231, 673, 1415, 1779
HpyCH4IV	ACGT	2 cut(s)	516, 1555
HpyCH4V	TGCA	8 cut(s)	42, 101, 353, 469, 664, 1073, 1262, 1590
HpyF10VI	GCNNNNNNNGC	3 cut(s)	48, 884, 935
HpyF3I	CTNAG	7 cut(s)	87, 117, 222, 297, 432, 480, 1500
HpySE526I	ACGT	2 cut(s)	516, 1555
HspAI	GCGC	2 cut(s)	881, 1525
KpnI	GGTACC	1 cut(s)	179
KspI	CCGCGG	1 cut(s)	49
Kzo9I	GATC	4 cut(s)	751, 894, 1215, 1432
LmnI	GCTCC	2 cut(s)	142, 490
Lsp1109I	GCAGC	6 cut(s)	139, 436, 865, 998, 1348, 1633
LweI	GCATC	6 cut(s)	4, 51, 594, 868, 1088, 1191
MaeI	CTAG	4 cut(s)	795, 1044, 1088, 1109
MaeII	ACGT	2 cut(s)	516, 1555
MaeIII	GTNAC	9 cut(s)	225, 231, 251, 309, 600, 770, 790, 1055, 1237
MalI	GATC	4 cut(s)	753, 896, 1217, 1434
MboI	GATC	4 cut(s)	751, 894, 1215, 1432
MboII	GAAGA	8 cut(s)	101, 983, 1007, 1225, 1291, 1451, 1667, 1771
MfeI	CAATTG	1 cut(s)	711
MflI	RGATCY	2 cut(s)	1215, 1432
MhlI	GDGCHC	2 cut(s)	1075, 1398
MlsI	TGGCCA	1 cut(s)	1568
MluCI	AATT	8 cut(s)	341, 405, 509, 711, 1152, 1256, 1268, 1754
MluNI	TGGCCA	1 cut(s)	1568
MlyI	GAGTC	5 cut(s)	317, 366, 471, 1477, 1541
MmeI	TCCRAC	1 cut(s)	657
Mox20I	TGGCCA	1 cut(s)	1568
MscI	TGGCCA	1 cut(s)	1568
MseI	TTAA	9 cut(s)	290, 441, 501, 729, 1134, 1314, 1452, 1593, 1647
MslI	CAYNNNNRTG	3 cut(s)	12, 864, 1554
Msp20I	TGGCCA	1 cut(s)	1568
MspA1I	CMGCKG	1 cut(s)	48
MspI	CCGG	3 cut(s)	179, 1244, 1714
MspR9I	CCNGG	2 cut(s)	180, 204
MunI	CAATTG	1 cut(s)	711
MvaI	CCWGG	1 cut(s)	204
MvnI	CGCG	1 cut(s)	48
MwoI	GCNNNNNNNGC	3 cut(s)	48, 884, 935
NciI	CCSGG	1 cut(s)	180
NcoI	CCATGG	1 cut(s)	775
NdeII	GATC	4 cut(s)	751, 894, 1215, 1432
NlaIV	GGNNCC	3 cut(s)	177, 620, 1434
NmuCI	GTSAC	6 cut(s)	231, 251, 309, 600, 1055, 1237
NsbI	TGCGCA	1 cut(s)	882
NspI	RCATGY	2 cut(s)	11, 923
PaqCI	CACCTGC	2 cut(s)	1615, 1720
PasI	CCCWGGG	1 cut(s)	203
PceI	AGGCCT	2 cut(s)	699, 1319
PfeI	GAWTC	3 cut(s)	135, 799, 1609
PflMI	CCANNNNNTGG	1 cut(s)	1148
PkrI	GCNGC	7 cut(s)	50, 154, 426, 880, 988, 1363, 1623
PleI	GAGTC	5 cut(s)	316, 366, 470, 1477, 1540
PpsI	GAGTC	5 cut(s)	316, 366, 470, 1477, 1540
PshBI	ATTAAT	1 cut(s)	501
Psp6I	CCWGG	1 cut(s)	202
PspEI	GGTNACC	1 cut(s)	770
PspGI	CCWGG	1 cut(s)	202
PspN4I	GGNNCC	3 cut(s)	177, 620, 1434
PspPI	GGNCC	5 cut(s)	618, 953, 1123, 1163, 1249
PsuI	RGATCY	2 cut(s)	1215, 1432
RsaI	GTAC	7 cut(s)	177, 402, 558, 670, 781, 1042, 1048
RsaNI	GTAC	7 cut(s)	176, 401, 557, 669, 780, 1041, 1047
RseI	CAYNNNNRTG	3 cut(s)	12, 864, 1554
SacII	CCGCGG	1 cut(s)	49
SaqAI	TTAA	9 cut(s)	290, 441, 501, 729, 1134, 1314, 1452, 1593, 1647
SatI	GCNGC	7 cut(s)	49, 153, 425, 879, 987, 1362, 1622
Sau3AI	GATC	4 cut(s)	751, 894, 1215, 1432
Sau96I	GGNCC	5 cut(s)	618, 953, 1123, 1163, 1249
ScaI	AGTACT	2 cut(s)	1042, 1048
SchI	GAGTC	5 cut(s)	317, 366, 471, 1477, 1541
ScrFI	CCNGG	2 cut(s)	180, 204
SduI	GDGCHC	2 cut(s)	1075, 1398
SfaNI	GCATC	6 cut(s)	4, 51, 594, 868, 1088, 1191
SfcI	CTRYAG	1 cut(s)	303
Sfr303I	CCGCGG	1 cut(s)	49
SgrBI	CCGCGG	1 cut(s)	49
SinI	GGWCC	1 cut(s)	618
SmiMI	CAYNNNNRTG	3 cut(s)	12, 864, 1554
SmlI	CTYRAG	3 cut(s)	1334, 1397, 1678
SmoI	CTYRAG	3 cut(s)	1334, 1397, 1678
SpeI	ACTAGT	1 cut(s)	1043
Sse9I	AATT	8 cut(s)	341, 405, 509, 711, 1152, 1256, 1268, 1754
SseBI	AGGCCT	2 cut(s)	699, 1319
SsiI	CCGC	2 cut(s)	46, 48
SspI	AATATT	1 cut(s)	1138
SspMI	CTAG	4 cut(s)	795, 1044, 1088, 1109
StuI	AGGCCT	2 cut(s)	699, 1319
StyD4I	CCNGG	2 cut(s)	178, 202
StyI	CCWWGG	2 cut(s)	700, 775
TaaI	ACNGT	4 cut(s)	231, 673, 1415, 1779
TaiI	ACGT	2 cut(s)	519, 1558
TaqI	TCGA	2 cut(s)	519, 1535
TasI	AATT	8 cut(s)	341, 405, 509, 711, 1152, 1256, 1268, 1754
TatI	WGTACW	3 cut(s)	668, 1040, 1046
TauI	GCSGC	1 cut(s)	51
TfiI	GAWTC	3 cut(s)	135, 799, 1609
Tru1I	TTAA	9 cut(s)	290, 441, 501, 729, 1134, 1314, 1452, 1593, 1647
Tru9I	TTAA	9 cut(s)	290, 441, 501, 729, 1134, 1314, 1452, 1593, 1647
TscAI	CASTG	5 cut(s)	609, 671, 678, 940, 1201
TseFI	GTSAC	6 cut(s)	231, 251, 309, 600, 1055, 1237
TseI	GCWGC	6 cut(s)	152, 424, 878, 986, 1361, 1621
Tsp45I	GTSAC	6 cut(s)	231, 251, 309, 600, 1055, 1237
TspDTI	ATGAA	9 cut(s)	185, 334, 434, 672, 842, 1019, 1281, 1340, 1674
TspGWI	ACGGA	2 cut(s)	547, 1074
TspRI	CASTG	5 cut(s)	609, 671, 678, 940, 1201
Van91I	CCANNNNNTGG	1 cut(s)	1148
VneI	GTGCAC	1 cut(s)	1071
VpaK11BI	GGWCC	1 cut(s)	618
VspI	ATTAAT	1 cut(s)	501
XagI	CCTNNNNNAGG	2 cut(s)	1337, 1727
XapI	RAATTY	2 cut(s)	341, 1256
XceI	RCATGY	2 cut(s)	11, 923
XspI	CTAG	4 cut(s)	795, 1044, 1088, 1109
ZrmI	AGTACT	2 cut(s)	1042, 1048

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.