Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6C

Orthogroup ID

OG0002582

Physical Location & Seq

Genomic Coordinates Forward (+)

35575995 .. 35584192

Gene Structure 8198 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6CG214100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3282 bp

ATGGGTCGGAAGAAGCCGACTGCTCGTGACGAAGAGAATCCGCCGCCAGCGGGTGGTGGTGGAGGAAAATCGAAAAAGAAGAACCTGGTGATCGAAGACGACGAGTATTCCGTCGGAACTGAGCTATCCGAGGAGAGTCAAGTCAAGGAAGAGAAGGTTCCTCCGCCTAGTAAAGGGAAGAAGGGCAAGAAGGGGAGTTCTAAAGCTTCTAAGGGAGTGGATGAGGACTTGGACGATGCCGACGAGGATGCAGATGAGGTTCCCCAAGTTGTTTTCACTGGGAAGAAGAAGGGCAAGTCGAAGAAGAGTGGCGGGAGTAGCGCATTCAGCTCGTCCAGTTTTGGGTTGCTTGAGGATGAGGATGATAAGGATGCTGAGGATGATGAGCAATCTGGTTTAACAGGCGATGATGAGGAGGAGGATGCTCCGGTGTTGAAATTTTCGGGGAAGAAGAAGCCTTCAAAGTCGACCAAGAAAACCGGGGCTAGTTCGTTTACAGGGTCGGCTTTTGATGCCATTGGTGAAGAAGGGGATAGTGATGGCGCGGTTGATGATGAGAGTAATGCAGCTGAAGATCATGAGAATGACGATGAGCCTGCAATTGCTTTTACAGGGAAAAAGAAGGCTTCCAAGGGTGGAAAGAAGGGTGGGAGTGTGTTTGCAGCAGCCAGCTTTAGTGCCCTTGATGATGCGGAGGAGGATAAGGATGAAAAAAACGATGAGGATGACGATATTCCTCAGATTACTTTCTCTGGTAAGAAAAAGAAGTCATCAAAGGCTTCAAAGAAGAGTGGCGGTAATTCTTTTAGCGCTGCATTGCTTGATGAGGAAGATGATGAAGATACTTCCGTTTCCAAAGCAACTAGTGCCGGTGATGATACAGTTGAGGATGAAGATGCATCTATGATAACATTCACAGGTAAGAAGAAGTCTTCAAAGAAGAAAGGTAATAGTGTGTTTACTGCACTGGGTGAAGATGCTGGGGTTGGGAGTGAAATTACAGATGTAGTTGAGCCCAAACAACCAACTACGGGAACTAGTAAAATTGAAGCTGATAATTCTAACGTTAGTAAGAATAAAGATCCAGAACCTTTAAAAAGTAAGAAAAAGAAGAAGAAGAGTGGAAGGACTGCTCAAGAAGAGGATGATTTGGATAAGATTCTTGCAGAGCTAGGTGAGCTTTCATCGAAACCTGCTGCTCCTCAGAAAGAGGAGACGGTGGAGGTTCAGCCTGCTGATTCTCAGAAAGAGGAGAAAGTTGTTGAGGTTCAACCTGATGCGGTTGCCCCAGTTGATGGTTCTGCTGCAAAGGAAGCTGAAGAAGAGACTGTGGAGTCTGCTGCAGCAAAGAAGAAGAAAAAGAAGAAGGAAAAGGAAAAGGAGAAAAAGGCGGCGGCAGCAGCAGCATCTGTAGGTGTCAAGGAGGAAAAACAAGAACAAGCAAAAATTGAGCCAGCAATTGAACCAAAGAAGAAGGAAGTGAAAGGCAAAGCAGCTGAAAAGAAGGTGCCTAAACATGTTAGGGAGATGCAGGAGGCCCTTGCTAGGCGACAGGAAGAAGAAGAGAAGAAGAAAAGGGAAGAAGAGGAGAAACTGAAGAAAGAAGAAGAGGAGAGGCATAAGCAGGAAGAACTTGAGAAGCAAAAGGAGGAGGCAAAGCGGAAGAAAAAGGAGAGGGAAAAGGAGAAGATCCAAGAGTTGAAGAAGGCAGGTTTATATAAAACTCCCAAGCAAAAAGAAGAGGAGAAGCGGCGGGAGCAAAAGAGACTCCAGTTACTTAGGGATTTACCTAATGCAGTGGATGGTGTGCCTCCTCCTACCACAGACAATGAAAAACCGGCTAAAAGACCCTTGTATCAGAAAAAGAAGTCAAAACCAGCACCCAATCAGCCAAATGGTGCAGCTGCTACGAAGCCTGTGGAAAGCACAGAAGGAATAAAAGAAGAAAACCAGCAGGTTACTGTTCCTGAGCTGGATTCTGTGGGAGTTGAGAAGGATGAAGAGCAGGAATCGGTTGAAGTAGCAGAAAAGCTATCAGAAGTTTCTGAATCTGTTAAAGAGAATGGATTGGAACAAGAAGAGGATGATGGTGATGATGATGAATGGGATGCAAAGAGCTGGGATGATGATGCGGTTGTTAATCTTTCATTTAAACGTGGGTTTTCTGATGAAGAGGTTGAACCTGTTGTGAATAAAGGGATGAAGCCTGCAGTGCAGAAAACCGTTCCTTCTCAACCAATAAAACCTCATGATGTTGAGAATCAGAAAAAGCAAGCTGAGGTGGAAGTTGACAAAGGTAGGAAGAAAGATGCTACTGCAAAGAAAGAAGCACCAAATTCAGATTCTGCCTCTAAACAGAGTGAAGATAATCTTCGCTCTCCCATTTGCTGCATTATGGGCCATGTTGATACTGGTAAAACTAAGCTTCTGGATTGTATCCGAGGCACAAATGTTCAGGAGGGTGAGGCTGGAGGTATTACTCAACAGATTGGTGCTACGTATTTTCCTGCGGATAATATCCGTGAGAGGACTAAAGAACTGAAAGCTGATGCAAAGCTGAAGGTCCCAGGTCTATTGGTCATTGATACCCCTGGCCACGAGTCATTTACTAATTTAAGGTCACGGGGTTCAGGTTTATGTGATATTGCAATTTTGGTTGTTGACATTATGCATGGCTTAGAGCCTCAAACAATAGAGTCACTCAATCTCTTGAAAATGAGGAATACCGAATTCATTGTTGCATTGAATAAGGTGGACAGGCTCTATGGGTGGAAAACCTGCCGCAACCAGCCAATAGGGAAGGCAATGAAGCAACAATCCAAGGATGTAGTAAATGAGTTCAATATGAGGGTTTCTCAGATTATCACTCAATTCATGGAGCAGGGCTTAAATACTGAATTGTATTATAAGAATAAAGAAATGGGAGAAACATACAGTATTGTACCTACAAGTGCCATTAGTGGTGAAGGGATTCCAGATTTGTTATTACTATTGATTCAGTGGGCCCAGAAAACTATGGTTGAGAAACTTACTTACAGCAATGAAATTCAGTGTACGGTATTGGAGGTCAAGGTTATTGAAGGTCTTGGGACAACAATTGATGTTGTGTTGGTTAATGGTGTGCTTCATGAAGGGGATCAAATAGTCGTTTGTGGCATGCAGGGACCTATTGTTACTTCAATTCGAGCTTTGTTGACACCACATCCCATGAAAGAACTTCGTGTCAAGGTATGGTTTTCATTTTCAGTTTTAATTTGTCTTGAGCTGTGTATTGGTTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001882 GO:0001883 GO:0002181 GO:0002183 GO:0003674 GO:0003676 GO:0003723 GO:0003743 GO:0003824 GO:0003924 GO:0005488 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006412 GO:0006413 GO:0006518 GO:0006807 GO:0008135 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019001 GO:0019538 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032991 GO:0033290 GO:0034641 GO:0034645 GO:0035639 GO:0036094 GO:0043043 GO:0043167 GO:0043168 GO:0043170 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0070993 GO:0071704 GO:0097159 GO:0097367 GO:1901265 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko00195 ko01100 ko03013 map00195 map01100 map03013

Pfam Domains

Protein Families

GTP_EFTU GTP_EFTU_D2 IF-2

Protein Analysis

1093

Amino Acids

119.61

Weight (kDa)

5.12

Isoelectric Point (pI)

45.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GTP_EFTU	PF00009	794 - 1003	3.1e-34	Elongation factor Tu GTP binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0002582)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G21160 AT1G76720 AT1G76720 AT1G76810 AT2G27700 AT2G27700
fragaria_vesca	FvH4_2g13940 FvH4_2g13940 FvH4_2g13940 FvH4_4g36100 FvH4_4g36312
malus_domestica	MD05G1023200.v1.1 MD09G1087100.v1.1 MD10G1024100.v1.1
prunus_persica	Prupe.2G099300_v2.0.a1 Prupe.8G027900_v2.0.a1
pyrus_communis	pycom05g01470 pycom09g01240 pycom10g01600
rosa_chinensis	RchiOBHm_Chr4g0445721 RchiOBHm_Chr6g0275701
rosa_laevigata	RLG00000005714 RLG00000013337 RLG00000013444
rosa_multiflora	Rmu_co8469881.1_g000001 Rmu_sc0003637.1_g000011 Rmu_sc0003637.1_g000012 Rmu_sc0007122.1_g000003
rosa_roxburghii	Rroxscaffold_7G00193000
rosa_rugosa	Rorug04G0365200 Rorug06G0095700
rosa_samantha	Rh4AG426100 Rh4CG453100 Rh4DG434300 Rh6AG207600 Rh6BG210300 Rh6CG214100 Rh6DG203400
rosa_wichuraiana	Rw4G036490 Rw6G018100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2907
AasI	GACNNNNNNGTC	1 cut(s)	1333
Acc36I	ACCTGC	4 cut(s)	1201, 1700, 1945, 2786
AccB1I	GGYRCC	1 cut(s)	1507
AccB7I	CCANNNNNTGG	2 cut(s)	53, 1295
AccI	GTMKAC	1 cut(s)	467
AccII	CGCG	1 cut(s)	545
AclI	AACGTT	1 cut(s)	1065
AclWI	GGATC	3 cut(s)	1076, 1684, 3144
AcoI	YGGCCR	1 cut(s)	2593
AcsI	RAATTY	4 cut(s)	437, 2335, 2729, 3045
AcuI	CTGAAG	4 cut(s)	591, 1338, 1616, 2579
AdeI	CACNNNGTG	1 cut(s)	971
AfaI	GTAC	2 cut(s)	2943, 3055
AfeI	AGCGCT	1 cut(s)	811
AfiI	CCNNNNNNNGG	7 cut(s)	50, 53, 173, 342, 1031, 1295, 1545
AflIII	ACRYGT	1 cut(s)	1516
AhlI	ACTAGT	2 cut(s)	863, 1037
AjnI	CCWGG	3 cut(s)	84, 2566, 2590
AjuI	GAANNNNNNNTTGG	2 cut(s)	2326, 2358
Alw26I	GTCTC	3 cut(s)	1208, 1319, 1759
AlwI	GGATC	3 cut(s)	1076, 1684, 3144
AlwNI	CAGNNNCTG	2 cut(s)	1409, 2345
Aor51HI	AGCGCT	1 cut(s)	811
AoxI	GGCC	4 cut(s)	1536, 2398, 2593, 3003
ApaI	GGGCCC	1 cut(s)	3007
ApoI	RAATTY	4 cut(s)	437, 2335, 2729, 3045
Asp700I	GAANNNNTTC	1 cut(s)	2970
AspLEI	GCGC	3 cut(s)	323, 545, 812
AspS9I	GGNCC	6 cut(s)	1537, 2398, 2563, 3003, 3004, 3164
AsuC2I	CCSGG	1 cut(s)	481
AsuHPI	GGTGA	8 cut(s)	100, 533, 884, 983, 1187, 2102, 2474, 2975
AvaII	GGWCC	2 cut(s)	2563, 3164
BaeGI	GKGCMC	2 cut(s)	682, 3007
BaeI	ACNNNNGTAYC	4 cut(s)	834, 867, 1838, 1871
BalI	TGGCCA	1 cut(s)	2595
BanI	GGYRCC	1 cut(s)	1507
BanII	GRGCYC	2 cut(s)	1017, 3007
BauI	CACGAG	2 cut(s)	24, 2597
BbsI	GAAGAC	2 cut(s)	102, 924
BbvCI	CCTCAGC	2 cut(s)	375, 2277
BccI	CCATC	4 cut(s)	533, 1289, 1796, 2081
BcgI	CGANNNNNNTGC	6 cut(s)	230, 264, 312, 346, 578, 612
BciT130I	CCWGG	3 cut(s)	86, 2568, 2592
BciVI	GTATCC	1 cut(s)	2447
BcnI	CCSGG	1 cut(s)	481
BcoDI	GTCTC	3 cut(s)	1208, 1319, 1759
BcuI	ACTAGT	2 cut(s)	863, 1037
BfaI	CTAG	6 cut(s)	168, 486, 864, 1038, 1172, 1545
BfmI	CTRYAG	3 cut(s)	1341, 1410, 2208
BfoI	RGCGCY	1 cut(s)	813
BfuAI	ACCTGC	4 cut(s)	1201, 1700, 1945, 2786
BfuI	GTATCC	1 cut(s)	2447
Bme1390I	CCNGG	4 cut(s)	86, 481, 2568, 2592
Bme18I	GGWCC	2 cut(s)	2563, 3164
BmgT120I	GGNCC	6 cut(s)	1537, 2398, 2563, 3003, 3004, 3164
BmiI	GGNNCC	6 cut(s)	159, 261, 1509, 2565, 3005, 3165
BmrFI	CCNGG	4 cut(s)	86, 481, 2568, 2592
BmrI	ACTGGG	3 cut(s)	288, 977, 1283
BmuI	ACTGGG	3 cut(s)	288, 977, 1283
BpiI	GAAGAC	2 cut(s)	102, 924
BplI	GAGNNNNNCTC	2 cut(s)	2839, 2871
BpmI	CTGGAG	2 cut(s)	1754, 2490
Bpu10I	CCTNAGC	3 cut(s)	375, 1968, 2277
BpuEI	CTTGAG	4 cut(s)	371, 1119, 1655, 3281
BpuMI	CCSGG	1 cut(s)	481
BsaAI	YACGTR	1 cut(s)	2499
BsaJI	CCNNGG	7 cut(s)	129, 480, 630, 2440, 2566, 2590, 2820
BsaWI	WCCGGW	1 cut(s)	427
Bsc4I	CCNNNNNNNGG	7 cut(s)	50, 53, 173, 342, 1031, 1295, 1545
Bse118I	RCCGGY	2 cut(s)	869, 1837
Bse1I	ACTGG	6 cut(s)	283, 336, 972, 1289, 1771, 2416
Bse3DI	GCAATG	3 cut(s)	815, 2811, 3046
BseBI	CCWGG	3 cut(s)	86, 2568, 2592
BseDI	CCNNGG	7 cut(s)	129, 480, 630, 2440, 2566, 2590, 2820
BseLI	CCNNNNNNNGG	7 cut(s)	50, 53, 173, 342, 1031, 1295, 1545
BseMI	GCAATG	3 cut(s)	815, 2811, 3046
BseMII	CTCAG	8 cut(s)	111, 366, 752, 1217, 1256, 1959, 2268, 2870
BseNI	ACTGG	6 cut(s)	283, 336, 972, 1289, 1771, 2416
BseSI	GKGCMC	2 cut(s)	682, 3007
BseYI	CCCAGC	2 cut(s)	980, 2118
BsgI	GTGCAG	3 cut(s)	948, 1920, 2234
Bsh1236I	CGCG	1 cut(s)	545
BshFI	GGCC	4 cut(s)	1538, 2400, 2595, 3005
BshNI	GGYRCC	1 cut(s)	1507
BsiSI	CCGG	4 cut(s)	428, 480, 870, 1838
BslFI	GGGAC	3 cut(s)	2549, 3103, 3177
BslI	CCNNNNNNNGG	7 cut(s)	50, 53, 173, 342, 1031, 1295, 1545
BsmAI	GTCTC	3 cut(s)	1208, 1319, 1759
BsmBI	CGTCTC	1 cut(s)	1208
BsmFI	GGGAC	3 cut(s)	2549, 3103, 3177
BsmI	GAATGC	1 cut(s)	323
BsnI	GGCC	4 cut(s)	1538, 2400, 2595, 3005
Bsp120I	GGGCCC	1 cut(s)	3003
Bsp1286I	GDGCHC	3 cut(s)	682, 1017, 3007
Bsp143I	GATC	5 cut(s)	90, 574, 1081, 1689, 3136
BspANI	GGCC	4 cut(s)	1538, 2400, 2595, 3005
BspCNI	CTCAG	8 cut(s)	112, 367, 751, 1216, 1255, 1960, 2269, 2869
BspFNI	CGCG	1 cut(s)	545
BspHI	TCATGA	3 cut(s)	577, 2248, 3127
BspLI	GGNNCC	6 cut(s)	159, 261, 1509, 2565, 3005, 3165
BspMAI	CTGCAG	2 cut(s)	1345, 2212
BspMI	ACCTGC	4 cut(s)	1201, 1700, 1945, 2786
BspPI	GGATC	3 cut(s)	1076, 1684, 3144
BspQI	GCTCTTC	1 cut(s)	1995
BspT107I	GGYRCC	1 cut(s)	1507
BsrDI	GCAATG	3 cut(s)	815, 2811, 3046
BsrFI	RCCGGY	2 cut(s)	869, 1837
BsrI	ACTGG	6 cut(s)	283, 336, 972, 1289, 1771, 2416
BssAI	RCCGGY	2 cut(s)	869, 1837
BssECI	CCNNGG	7 cut(s)	129, 480, 630, 2440, 2566, 2590, 2820
BssMI	GATC	5 cut(s)	90, 574, 1081, 1689, 3136
BssSI	CACGAG	2 cut(s)	24, 2597
BssT1I	CCWWGG	2 cut(s)	630, 2820
Bst2BI	CACGAG	2 cut(s)	24, 2597
Bst2UI	CCWGG	3 cut(s)	86, 2568, 2592
Bst4CI	ACNGT	7 cut(s)	883, 1219, 1330, 1963, 2224, 2936, 3058
BstAPI	GCANNNNNTGC	1 cut(s)	866
BstBAI	YACGTR	1 cut(s)	2499
BstC8I	GCNNGC	8 cut(s)	48, 597, 670, 1233, 1455, 2208, 2274, 3158
BstENI	CCTNNNNNAGG	1 cut(s)	171
BstFNI	CGCG	1 cut(s)	545
BstH2I	RGCGCY	1 cut(s)	813
BstHHI	GCGC	3 cut(s)	323, 545, 812
BstKTI	GATC	5 cut(s)	93, 577, 1084, 1692, 3139
BstMAI	GTCTC	3 cut(s)	1208, 1319, 1759
BstMBI	GATC	5 cut(s)	90, 574, 1081, 1689, 3136
BstNI	CCWGG	3 cut(s)	86, 2568, 2592
BstNSI	RCATGY	2 cut(s)	1520, 3160
BstSCI	CCNGG	4 cut(s)	84, 479, 2566, 2590
BstSFI	CTRYAG	3 cut(s)	1341, 1410, 2208
BstSLI	GKGCMC	2 cut(s)	682, 3007
BstSNI	TACGTA	1 cut(s)	2499
BstUI	CGCG	1 cut(s)	545
BstV2I	GAAGAC	2 cut(s)	102, 924
BstX2I	RGATCY	2 cut(s)	1081, 1689
BstYI	RGATCY	2 cut(s)	1081, 1689
BsuI	GTATCC	1 cut(s)	2447
BsuRI	GGCC	4 cut(s)	1538, 2400, 2595, 3005
BtgZI	GCGATG	1 cut(s)	420
BtsI	GCAGTG	2 cut(s)	1803, 2217
BtsIMutI	CAGTG	6 cut(s)	276, 965, 1803, 2217, 3005, 3056
BveI	ACCTGC	4 cut(s)	1201, 1700, 1945, 2786
Cac8I	GCNNGC	8 cut(s)	48, 597, 670, 1233, 1455, 2208, 2274, 3158
CaiI	CAGNNNCTG	2 cut(s)	1409, 2345
CciI	TCATGA	3 cut(s)	577, 2248, 3127
CfoI	GCGC	3 cut(s)	323, 545, 812
Cfr10I	RCCGGY	2 cut(s)	869, 1837
Cfr13I	GGNCC	6 cut(s)	1537, 2398, 2563, 3003, 3004, 3164
CsiI	ACCWGGT	1 cut(s)	84
Csp6I	GTAC	2 cut(s)	2942, 3054
CviAII	CATG	9 cut(s)	578, 1517, 2249, 2402, 2672, 2875, 3128, 3157, 3208
CviQI	GTAC	2 cut(s)	2942, 3054
DpnI	GATC	5 cut(s)	92, 576, 1083, 1691, 3138
DpnII	GATC	5 cut(s)	90, 574, 1081, 1689, 3136
DraI	TTTAAA	2 cut(s)	1095, 2152
DraIII	CACNNNGTG	1 cut(s)	971
DrdI	GACNNNNNNGTC	1 cut(s)	1333
DseDI	GACNNNNNNGTC	1 cut(s)	1333
EaeI	YGGCCR	1 cut(s)	2593
EciI	GGCGGA	2 cut(s)	30, 153
Eco105I	TACGTA	1 cut(s)	2499
Eco130I	CCWWGG	2 cut(s)	630, 2820
Eco24I	GRGCYC	2 cut(s)	1017, 3007
Eco47I	GGWCC	2 cut(s)	2563, 3164
Eco47III	AGCGCT	1 cut(s)	811
Eco57I	CTGAAG	4 cut(s)	591, 1338, 1616, 2579
EcoNI	CCTNNNNNAGG	1 cut(s)	171
EcoO109I	RGGNCCY	3 cut(s)	1537, 2563, 3164
EcoRI	GAATTC	1 cut(s)	2729
EcoRII	CCWGG	3 cut(s)	84, 2566, 2590
EcoT14I	CCWWGG	2 cut(s)	630, 2820
EcoT22I	ATGCAT	2 cut(s)	901, 2673
EcoT38I	GRGCYC	2 cut(s)	1017, 3007
ErhI	CCWWGG	2 cut(s)	630, 2820
Esp3I	CGTCTC	1 cut(s)	1208
FaeI	CATG	9 cut(s)	581, 1520, 2252, 2405, 2675, 2878, 3131, 3160, 3211
FalI	AAGNNNNNCTT	2 cut(s)	2355, 2387
FaqI	GGGAC	3 cut(s)	2549, 3103, 3177
FatI	CATG	9 cut(s)	577, 1516, 2248, 2401, 2671, 2874, 3127, 3156, 3207
FauI	CCCGC	3 cut(s)	43, 305, 1746
FblI	GTMKAC	1 cut(s)	467
FriOI	GRGCYC	2 cut(s)	1017, 3007
FspBI	CTAG	6 cut(s)	168, 486, 864, 1038, 1172, 1545
GlaI	GCGC	3 cut(s)	322, 544, 811
GsaI	CCCAGC	2 cut(s)	984, 2122
GsuI	CTGGAG	2 cut(s)	1754, 2490
HaeII	RGCGCY	1 cut(s)	813
HaeIII	GGCC	4 cut(s)	1538, 2400, 2595, 3005
HapII	CCGG	4 cut(s)	428, 480, 870, 1838
HhaI	GCGC	3 cut(s)	323, 545, 812
Hin1II	CATG	9 cut(s)	581, 1520, 2252, 2405, 2675, 2878, 3131, 3160, 3211
Hin6I	GCGC	3 cut(s)	321, 543, 810
HinP1I	GCGC	3 cut(s)	321, 543, 810
HincII	GTYRAC	4 cut(s)	468, 2290, 2662, 3195
HindII	GTYRAC	4 cut(s)	468, 2290, 2662, 3195
HindIII	AAGCTT	2 cut(s)	204, 2423
HpaII	CCGG	4 cut(s)	428, 480, 870, 1838
HphI	GGTGA	8 cut(s)	100, 533, 884, 983, 1187, 2102, 2474, 2975
Hpy166II	GTNNAC	8 cut(s)	468, 495, 960, 2290, 2662, 2755, 3054, 3195
Hpy8I	GTNNAC	8 cut(s)	468, 495, 960, 2290, 2662, 2755, 3054, 3195
Hpy99I	CGWCG	3 cut(s)	104, 116, 245
HpyCH4III	ACNGT	7 cut(s)	883, 1219, 1330, 1963, 2224, 2936, 3058
HpyCH4IV	ACGT	3 cut(s)	1065, 2155, 2498
HpySE526I	ACGT	3 cut(s)	1065, 2155, 2498
Hsp92II	CATG	9 cut(s)	581, 1520, 2252, 2405, 2675, 2878, 3131, 3160, 3211
HspAI	GCGC	3 cut(s)	321, 543, 810
Kzo9I	GATC	5 cut(s)	90, 574, 1081, 1689, 3136
LguI	GCTCTTC	1 cut(s)	1995
LmnI	GCTCC	4 cut(s)	430, 1204, 1756, 2878
MabI	ACCWGGT	1 cut(s)	84
MaeI	CTAG	6 cut(s)	168, 486, 864, 1038, 1172, 1545
MaeII	ACGT	3 cut(s)	1065, 2155, 2498
MaeIII	GTNAC	6 cut(s)	26, 1773, 1957, 2619, 2697, 3172
MalI	GATC	5 cut(s)	92, 576, 1083, 1691, 3138
MboI	GATC	5 cut(s)	90, 574, 1081, 1689, 3136
MfeI	CAATTG	3 cut(s)	600, 1458, 3096
MflI	RGATCY	2 cut(s)	1081, 1689
MhlI	GDGCHC	3 cut(s)	682, 1017, 3007
MlsI	TGGCCA	1 cut(s)	2595
MluNI	TGGCCA	1 cut(s)	2595
MlyI	GAGTC	5 cut(s)	145, 1343, 1761, 2609, 2705
MmeI	TCCRAC	1 cut(s)	94
Mox20I	TGGCCA	1 cut(s)	2595
Mph1103I	ATGCAT	2 cut(s)	901, 2673
MroXI	GAANNNNTTC	1 cut(s)	2970
MscI	TGGCCA	1 cut(s)	2595
MseI	TTAA	9 cut(s)	398, 1094, 2055, 2139, 2151, 2615, 2888, 3114, 3251
MslI	CAYNNNNRTG	1 cut(s)	582
Msp20I	TGGCCA	1 cut(s)	2595
MspA1I	CMGCKG	4 cut(s)	50, 569, 1496, 1904
MspI	CCGG	4 cut(s)	428, 480, 870, 1838
MspR9I	CCNGG	4 cut(s)	86, 481, 2568, 2592
MunI	CAATTG	3 cut(s)	600, 1458, 3096
Mva1269I	GAATGC	1 cut(s)	323
MvaI	CCWGG	3 cut(s)	86, 2568, 2592
MvnI	CGCG	1 cut(s)	545
NciI	CCSGG	1 cut(s)	481
NdeII	GATC	5 cut(s)	90, 574, 1081, 1689, 3136
NlaIII	CATG	9 cut(s)	581, 1520, 2252, 2405, 2675, 2878, 3131, 3160, 3211
NlaIV	GGNNCC	6 cut(s)	159, 261, 1509, 2565, 3005, 3165
NmuCI	GTSAC	3 cut(s)	26, 2619, 2697
NsiI	ATGCAT	2 cut(s)	901, 2673
NspI	RCATGY	2 cut(s)	1520, 3160
PaeI	GCATGC	1 cut(s)	3160
PagI	TCATGA	3 cut(s)	577, 2248, 3127
PciI	ACATGT	1 cut(s)	1516
PciSI	GCTCTTC	1 cut(s)	1995
PcsI	WCGNNNNNNNCGW	3 cut(s)	99, 108, 240
PctI	GAATGC	1 cut(s)	323
PdmI	GAANNNNTTC	1 cut(s)	2970
PflMI	CCANNNNNTGG	2 cut(s)	53, 1295
PleI	GAGTC	5 cut(s)	144, 1342, 1761, 2608, 2704
PpsI	GAGTC	5 cut(s)	144, 1342, 1761, 2608, 2704
Ppu21I	YACGTR	1 cut(s)	2499
PpuMI	RGGWCCY	2 cut(s)	2563, 3164
PscI	ACATGT	1 cut(s)	1516
PsiI	TTATAA	1 cut(s)	2907
Psp1406I	AACGTT	1 cut(s)	1065
Psp5II	RGGWCCY	2 cut(s)	2563, 3164
Psp6I	CCWGG	3 cut(s)	84, 2566, 2590
PspFI	CCCAGC	2 cut(s)	980, 2118
PspGI	CCWGG	3 cut(s)	84, 2566, 2590
PspN4I	GGNNCC	6 cut(s)	159, 261, 1509, 2565, 3005, 3165
PspOMI	GGGCCC	1 cut(s)	3003
PspPI	GGNCC	6 cut(s)	1537, 2398, 2563, 3003, 3004, 3164
PspPPI	RGGWCCY	2 cut(s)	2563, 3164
PstI	CTGCAG	2 cut(s)	1345, 2212
PstNI	CAGNNNCTG	2 cut(s)	1409, 2345
PsuI	RGATCY	2 cut(s)	1081, 1689
PvuII	CAGCTG	3 cut(s)	569, 1496, 1904
RsaI	GTAC	2 cut(s)	2943, 3055
RsaNI	GTAC	2 cut(s)	2942, 3054
RseI	CAYNNNNRTG	1 cut(s)	582
SalI	GTCGAC	1 cut(s)	466
SapI	GCTCTTC	1 cut(s)	1995
SaqAI	TTAA	9 cut(s)	398, 1094, 2055, 2139, 2151, 2615, 2888, 3114, 3251
Sau3AI	GATC	5 cut(s)	90, 574, 1081, 1689, 3136
Sau96I	GGNCC	6 cut(s)	1537, 2398, 2563, 3003, 3004, 3164
SchI	GAGTC	5 cut(s)	145, 1343, 1761, 2609, 2705
ScrFI	CCNGG	4 cut(s)	86, 481, 2568, 2592
SduI	GDGCHC	3 cut(s)	682, 1017, 3007
SexAI	ACCWGGT	1 cut(s)	84
SfcI	CTRYAG	3 cut(s)	1341, 1410, 2208
SinI	GGWCC	2 cut(s)	2563, 3164
SmiMI	CAYNNNNRTG	1 cut(s)	582
SmlI	CTYRAG	4 cut(s)	350, 1134, 1634, 3260
SmoI	CTYRAG	4 cut(s)	350, 1134, 1634, 3260
SnaBI	TACGTA	1 cut(s)	2499
SpeI	ACTAGT	2 cut(s)	863, 1037
SphI	GCATGC	1 cut(s)	3160
SspMI	CTAG	6 cut(s)	168, 486, 864, 1038, 1172, 1545
StyD4I	CCNGG	4 cut(s)	84, 479, 2566, 2590
StyI	CCWWGG	2 cut(s)	630, 2820
TaaI	ACNGT	7 cut(s)	883, 1219, 1330, 1963, 2224, 2936, 3058
TaiI	ACGT	3 cut(s)	1068, 2158, 2501
TaqI	TCGA	6 cut(s)	71, 93, 299, 467, 1187, 3184
TauI	GCSGC	5 cut(s)	46, 1394, 1397, 1753, 2784
Tru1I	TTAA	9 cut(s)	398, 1094, 2055, 2139, 2151, 2615, 2888, 3114, 3251
Tru9I	TTAA	9 cut(s)	398, 1094, 2055, 2139, 2151, 2615, 2888, 3114, 3251
TscAI	CASTG	6 cut(s)	283, 972, 1803, 2217, 3005, 3056
TseFI	GTSAC	3 cut(s)	26, 2619, 2697
Tsp45I	GTSAC	3 cut(s)	26, 2619, 2697
TspGWI	ACGGA	3 cut(s)	100, 838, 2510
TspRI	CASTG	6 cut(s)	283, 972, 1803, 2217, 3005, 3056
Van91I	CCANNNNNTGG	2 cut(s)	53, 1295
VpaK11BI	GGWCC	2 cut(s)	2563, 3164
XagI	CCTNNNNNAGG	1 cut(s)	171
XapI	RAATTY	4 cut(s)	437, 2335, 2729, 3045
XceI	RCATGY	2 cut(s)	1520, 3160
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2408
XmiI	GTMKAC	1 cut(s)	467
XmnI	GAANNNNTTC	1 cut(s)	2970
XspI	CTAG	6 cut(s)	168, 486, 864, 1038, 1172, 1545
Zsp2I	ATGCAT	2 cut(s)	901, 2673

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.