AT1G76720

Contains the following InterPro domains Small GTP-binding protein (InterPro IPR005225), Translation elongation factor EFTu EF1A, domain 2 (InterPro IPR004161), Translation initiation factor 2 related (InterPro IPR015760), Protein synthesis factor, GTP-binding (InterPro IPR000795)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0002582
Physical Location & Seq
Reverse (-)
28794144 .. 28800402
6259 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G76720.2

Sequence Viewer

Length: 3576 bp
ATGGGTCGGAAGAAGCCGAGTAGTTCTCGAGGTGGAGATGATGAGCAACCACCTGCTGCGTCATCTCTCGTTGGTGCTGAATCCAAGAAGAAAGCTGTCCAAAGTGATGAAGAAGATAAGTACTCCATAAATACTGAGGAAGAGAAAGTTGTTATCACTGGGAAGAAGAAGAGTAATAAGAAGGTTACGCAGAAGCATGATGACGACGATGATTTTACTGAGGCTGTTCCCGAGAATGGTTTTGTAGGGAAGAAGAAGAAGTCCAAAGGTAAGAACAGAGGTGGCTCTGTTAGCTTTGCCCTGCTTTCTGGTAAGGAGGAAACGGATGATAATGAATCTAATGGGGACAAAGATGATGAGCCAGTTATAAGTTTTACAGGTAAGAAGAATGCGTCTAACAAGGGTAAAAAAGGCTTTGCGGTTTCAGCTTTTGATGCCCTTGGTGGTGATAAGGATGACGAGGAAGTGGATGGAGATGAGGAACAAGTTTCACCTATCACTTTCTCTGAAGGGACAATGGGAGACGAAGAGAGCCTCGAAATCACGTTTTCCGGTAAAAAGAAGGGCAGCATTGTTTTGGCCTCACTAGGTGATGACTCAGTTGCCGACGAAACTAGTCAAGCTAAAACTCCTGATACTAAGAGTGTGGAGGTTATAGAAACTGGAAAGATCAAGAAAAAGAAGAAGAATAAGAATAAGGTTGCAAGGACATTAGAGGAAGAGGATGATTTGGACAAACTTCTTGCAGAACTTGGAGAAACCCCTGCTGCTGAAAGACCTGCTTCATCGACACCCGAAGTAGAGAAAGTTCAAGCTCAGCCTGGGCCAGTTGCACCTGTAGAGAATGCTGGTGAGAAGGAGGGTGAAAAAGAGACTGTTGAGACTGCTGCAGCGAAGAAAAAGAAAAAGAAGAAGGAGAAAGACAAGGAAAAAAAAGCAGCTGCTGCAGCAACCTCTTCTGTAGAGGCTAAGGAGGAAAAACAAGAAGAATCAGTTACTGAGCCACTACAGCCGAAGAAGAAGGATGCAAAGGGTAAAGCAGCAGAGAAGAAAATTCCTAAACATGTTAGAGAGATGCAAGAGGCTCTTGCACGGCGGCAAGAAGCAGAAGAGAGAAAAAAGAAGGAAGAAGAAGAAAAATTAAGGAAGGAGGAAGAGGAGCGCCGCAGGCAGGAAGAGCTTGAGGCTCAGGCTGAGGAGGCTAAGCGTAAGAGAAAGGAAAAGGAAAAGGAGAAACTGTTGAGGAAGAAGCTAGAGGGCAAGCTTCTAACAGCAAAGCAAAAGACTGAAGCTCAAAAGAGGGAGGCGTTTAAGAACCAGCTGCTGGCTGCTGGTAGAGGTCTTCCTGTTGCAGATGATGTTGGTGATGCTACTTCCTCAAAGCGTCCCATTTATGCCAACAAGAAAAAACCATCTCGCCAGAAAGGCAATGACACTTCTGTCCAGGTGGAAGATGAGGTAGAGCCACAAGAAAACCATGCAGCTACTTTAGGTGAAGTGGGTTCAGAAGACACTGAAAAGGTTGATTTACTAGAGTCAGCAAACACAGGCGAGAAATCAGGTCCTGCTGATGTAGCTCAGGAGAATGGGGTTGAGGAAGATGATGAATGGGATGCAAAAAGCTGGGATAATGTTGATCTTAAAATTGACGATAAAGAGGAAGAAGCTCAGCCTGTGGTTAAGAAAGAATTAAAGGCACATGATTCAGACCATGAAACTGAAAAGCCAACAGCTAAACCAGCAGGTATGAGCAAGCTAACAACAGGTGCGGTAAAAGCTATTTCTGAAGTAGAAGATGCTGCTACACAGACAAAACGTGCTAAGAAGGGTAAATGCTTAGCTCCAAGTGAATTTATAAAAGAAGGTGGAGAAAATCTCCGTTCTATTATTTGCTGTATCATGGGTCATGTCGATAGTGGTAAAACAAAGCTGTTGGATTGCATCAGAGGAACAAATGTACAGGAAGGTGAGGCTGGAGGTATAACTCAGCAGATTGGTGCAACATATTTCCCTGCCAAAAACATCCGAGAGAGGACCAGGGAACTGAAAGCGGATGCAAAACTTAAGGTGCCAGGTCTATTGGTTATCGATACACCTGGCCATGAGTCCTTTACTAATCTGCGGTCAAGAGGTTCAAGTTTGTGCGACCTTGCAATTTTGGTGGTTGACATAAAGCATGGGTTAGAGCCACAAACCATAGAATCTCTAAATCTTTTGAGGATGAGGAATACAGAGTTCATTATTGCATTGAATAAGGTGGATAGGCTATATGGATGGAAAACATGCAAGAATGCTCCTATCGTGAAGGCAATGAAGCAGCAAAATAAAGATGTTACGAATGAATTCAACATAAGGCTGACTGATATTATAACCCAATTCAAGGAGCAAGGACTCAACACTGAGATTTATTACAAGAATAAAGAAATGGGAGAAACTTTCAGTATTGTTCCTACTAGTGCTAAAAGTGGAGAAGGGGTCCCAGATCTCTTGCTGTGGTTGGTTCAATGGGCTCAGAAAACAATGGTTGAGAAACTTACATATGTTGACGAAGTGCAGTGTACTGTACTTGAGGTCAAAGTTATTGAAGGCCATGGGACAACGATTGATGTTGTGTTGGTAAACGGTGAACTCCATGAAGGTGATCAAATCGTCGTTTGTGGGCTACAGGGACCTATTGTCACAACGATTAGAGCATTACTGACTCCTCATCCAATGAAGGAGTTAAGGGTGAAGGGTACTTATCTGCATCACAAAGAAATAAAGGCTGCACAAGGCATCAAAATTACTGCCCAGGGCCTTGAACACGCAATTGCTGGTACTTCCCTGCATGTGGTTGGACCTGATGATGACATCGAGGCAATGAAGGAGTCGGCTATGGAAGATATGGAATCGGTTTTGAGCAGGATTGACAAAAGTGGTGAAGGAGTTTATGTACAAACGTCTACATTAGGGTCATTAGAAGCATTGCTTGAATTCTTGAAGACCCCAGCTGTAAATATACCTGTCAGTGAAGGATATAATGAAGGCTGGAGTGATGCTCGAGAAGAAAAGGAATATGCTACAATTTTGGCTTTTGATGTGAAAGTGACTACAGAGGCTCGTGAACTTGCAGACGAAATGGGAGTAAAAATCTTCTGCGCTGATATCATCTATCAGTTATTCAATCAGTTTCAGGTTTACATTGAGAATATCAAAGAGGAGAAAAAGAAGGAATCAGCCGGTGAGGCAGTCTTCCCATGTGTCCTTCAGATTTTACCTAACTGTGTGTTCAATAAGAGAGACCCAATAATCCTTGGAGTTAAGGTCCACGACGGTATACTTAAGATCGGAACCCCCATTTGCGTCCCGGGCAGAGAGTTCACAGATATTGGCCGCATTGCCTCTATTGAGAACAACCACAAGCCTGTTGACTATGCAGAGAAGGGCGACGAGGTGGCGATTAAGATTGTTGCTTCAAATCGCGAAGAACAGAAGATGTTTGGAAGGCATTTTGACATGGAAGATGAGCTTGTGAGTCACATTTCGAGGAGATCTATCGACATACTCAAAGCTGACTACATGGTAAGTCAAGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 ko03013 map00195 map01100 map03013
Pfam Domains
Protein Families
GTP_EFTU GTP_EFTU_D2 IF-2

Protein Analysis

1191

Amino Acids

131.36

Weight (kDa)

5.4

Isoelectric Point (pI)

39.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 661 - 871 1.7e-34 Elongation factor Tu GTP binding domain
MMR_HSR1 PF01926 665 - 785 3e-06 50S ribosome-binding GTPase
GTP_EFTU_D2 PF03144 898 - 973 1e-05 Elongation factor Tu domain 2
IF-2 PF11987 986 - 1090 2.6e-14 Translation-initiation factor 2
GTP_EFTU_D4 PF14578 1112 - 1203 2.1e-15 Elongation factor Tu domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 368, 1856, 2369
AarI CACCTGC 1 cut(s) 61
AasI GACNNNNNNGTC 1 cut(s) 1439
Acc36I ACCTGC 3 cut(s) 61, 787, 1733
AccB1I GGYRCC 1 cut(s) 2068
AccB7I CCANNNNNTGG 1 cut(s) 1324
AccI GTMKAC 2 cut(s) 2942, 3316
AccII CGCG 1 cut(s) 3462
AciI CCGC 7 cut(s) 419, 1096, 1165, 1769, 2051, 2122, 3373
AcoI YGGCCR 2 cut(s) 2098, 3370
AcsI RAATTY 4 cut(s) 1053, 1850, 2342, 2972
AcuI CTGAAG 4 cut(s) 528, 1308, 1806, 3230
AdeI CACNNNGTG 1 cut(s) 590
AfaI GTAC 7 cut(s) 122, 1959, 2560, 2565, 2737, 2818, 2934
AfiI CCNNNNNNNGG 5 cut(s) 236, 1171, 1324, 2380, 2830
AflII CTTAAG 2 cut(s) 2063, 3320
AflIII ACRYGT 1 cut(s) 1063
AhdI GACNNNNNGTC 1 cut(s) 2675
AhlI ACTAGT 2 cut(s) 614, 2453
AjnI CCWGG 6 cut(s) 820, 1443, 2036, 2071, 2095, 2790
Alw26I GTCTC 4 cut(s) 516, 866, 875, 3273
AlwNI CAGNNNCTG 4 cut(s) 944, 998, 1324, 1565
Ama87I CYCGRG 4 cut(s) 27, 230, 3039, 3346
AoxI GGCC 6 cut(s) 579, 824, 2098, 2587, 2794, 3370
ApoI RAATTY 4 cut(s) 1053, 1850, 2342, 2972
Asp700I GAANNNNTTC 1 cut(s) 2342
AspLEI GCGC 2 cut(s) 1164, 3140
AspS9I GGNCC 8 cut(s) 824, 1562, 2034, 2476, 2669, 2794, 2837, 3304
AsuC2I CCSGG 2 cut(s) 3347, 3348
AvaI CYCGRG 4 cut(s) 27, 230, 3039, 3346
AvaII GGWCC 6 cut(s) 1562, 2034, 2476, 2669, 2837, 3304
BalI TGGCCA 1 cut(s) 2100
BanI GGYRCC 1 cut(s) 2068
BanII GRGCYC 1 cut(s) 2512
BauI CACGAG 1 cut(s) 3099
BbsI GAAGAC 4 cut(s) 1334, 1515, 2987, 3223
BbvCI CCTCAGC 1 cut(s) 1194
BccI CCATC 3 cut(s) 464, 1420, 2268
BceAI ACGGC 1 cut(s) 1109
BciT130I CCWGG 6 cut(s) 822, 1445, 2038, 2073, 2097, 2792
BclI TGATCA 1 cut(s) 2641
BcnI CCSGG 2 cut(s) 3347, 3348
BcoDI GTCTC 4 cut(s) 516, 866, 875, 3273
BcuI ACTAGT 2 cut(s) 614, 2453
BfaI CTAG 5 cut(s) 587, 615, 1253, 1532, 2454
BfmI CTRYAG 7 cut(s) 837, 888, 945, 960, 1007, 2663, 3090
BfoI RGCGCY 1 cut(s) 1165
BfrI CTTAAG 2 cut(s) 2063, 3320
BfuAI ACCTGC 3 cut(s) 61, 787, 1733
BglI GCCNNNNNGGC 2 cut(s) 1425, 3224
BglII AGATCT 2 cut(s) 2482, 3530
BlpI GCTNAGC 4 cut(s) 816, 1203, 1668, 1837
BmcAI AGTACT 1 cut(s) 122
Bme1390I CCNGG 8 cut(s) 822, 1445, 2038, 2073, 2097, 2792, 3347, 3348
Bme18I GGWCC 6 cut(s) 1562, 2034, 2476, 2669, 2837, 3304
BmeRI GACNNNNNGTC 1 cut(s) 2675
BmeT110I CYCGRG 4 cut(s) 27, 230, 3039, 3346
BmgT120I GGNCC 8 cut(s) 824, 1562, 2034, 2476, 2669, 2794, 2837, 3304
BmiI GGNNCC 5 cut(s) 2070, 2477, 2478, 2670, 3331
BmrFI CCNGG 8 cut(s) 822, 1445, 2038, 2073, 2097, 2792, 3347, 3348
BmrI ACTGGG 1 cut(s) 168
BmuI ACTGGG 1 cut(s) 168
BpiI GAAGAC 4 cut(s) 1334, 1515, 2987, 3223
BplI GAGNNNNNCTC 6 cut(s) 10, 42, 1860, 1892, 3022, 3054
BpmI CTGGAG 2 cut(s) 1995, 3049
Bpu10I CCTNAGC 4 cut(s) 969, 1188, 1194, 1578
Bpu1102I GCTNAGC 4 cut(s) 816, 1203, 1668, 1837
BpuEI CTTGAG 2 cut(s) 1202, 2588
BpuMI CCSGG 2 cut(s) 3347, 3348
Bsa29I ATCGAT 1 cut(s) 2088
BsaI GGTCTC 1 cut(s) 3273
BsaJI CCNNGG 8 cut(s) 439, 821, 2037, 2590, 2790, 2791, 3292, 3346
BsaWI WCCGGW 1 cut(s) 551
BsaXI ACNNNNNCTCC 8 cut(s) 1223, 1253, 2460, 2490, 2916, 2946, 3288, 3318
Bsc4I CCNNNNNNNGG 5 cut(s) 236, 1171, 1324, 2380, 2830
Bse118I RCCGGY 1 cut(s) 3218
Bse1I ACTGG 4 cut(s) 163, 362, 667, 827
Bse3DI GCAATG 5 cut(s) 1435, 2316, 2865, 2963, 3375
BseBI CCWGG 6 cut(s) 822, 1445, 2038, 2073, 2097, 2792
BseCI ATCGAT 1 cut(s) 2088
BseDI CCNNGG 8 cut(s) 439, 821, 2037, 2590, 2790, 2791, 3292, 3346
BseLI CCNNNNNNNGG 5 cut(s) 236, 1171, 1324, 2380, 2830
BseMI GCAATG 5 cut(s) 1435, 2316, 2865, 2963, 3375
BseNI ACTGG 4 cut(s) 163, 362, 667, 827
BseRI GAGGAG 5 cut(s) 1172, 1211, 2694, 3212, 3541
BseYI CCCAGC 2 cut(s) 1623, 2986
BsgI GTGCAG 2 cut(s) 2573, 2751
Bsh1236I CGCG 1 cut(s) 3462
BshFI GGCC 6 cut(s) 581, 826, 2100, 2589, 2796, 3372
BshNI GGYRCC 1 cut(s) 2068
BshVI ATCGAT 1 cut(s) 2088
BsiHKCI CYCGRG 4 cut(s) 27, 230, 3039, 3346
BsiSI CCGG 3 cut(s) 552, 3219, 3347
BslFI GGGAC 7 cut(s) 359, 526, 1371, 2462, 2608, 2682, 3329
BslI CCNNNNNNNGG 5 cut(s) 236, 1171, 1324, 2380, 2830
BsmAI GTCTC 4 cut(s) 516, 866, 875, 3273
BsmBI CGTCTC 1 cut(s) 516
BsmFI GGGAC 7 cut(s) 359, 526, 1371, 2462, 2608, 2682, 3329
BsmI GAATGC 3 cut(s) 394, 850, 2296
BsnI GGCC 6 cut(s) 581, 826, 2100, 2589, 2796, 3372
Bso31I GGTCTC 1 cut(s) 3273
BsoBI CYCGRG 4 cut(s) 27, 230, 3039, 3346
Bsp1286I GDGCHC 1 cut(s) 2512
Bsp1407I TGTACA 2 cut(s) 1957, 2932
Bsp143I GATC 6 cut(s) 669, 1636, 2482, 2641, 3324, 3530
Bsp1720I GCTNAGC 4 cut(s) 816, 1203, 1668, 1837
Bsp19I CCATGG 1 cut(s) 2590
Bsp68I TCGCGA 1 cut(s) 3462
BspACI CCGC 7 cut(s) 419, 1096, 1165, 1769, 2051, 2122, 3373
BspANI GGCC 6 cut(s) 581, 826, 2100, 2589, 2796, 3372
BspDI ATCGAT 1 cut(s) 2088
BspFNI CGCG 1 cut(s) 3462
BspLI GGNNCC 5 cut(s) 2070, 2477, 2478, 2670, 3331
BspMAI CTGCAG 2 cut(s) 892, 949
BspMI ACCTGC 3 cut(s) 61, 787, 1733
BspQI GCTCTTC 1 cut(s) 1170
BspT107I GGYRCC 1 cut(s) 2068
BspTI CTTAAG 2 cut(s) 2063, 3320
BspTNI GGTCTC 1 cut(s) 3273
BsrDI GCAATG 5 cut(s) 1435, 2316, 2865, 2963, 3375
BsrFI RCCGGY 1 cut(s) 3218
BsrGI TGTACA 2 cut(s) 1957, 2932
BsrI ACTGG 4 cut(s) 163, 362, 667, 827
BssAI RCCGGY 1 cut(s) 3218
BssECI CCNNGG 8 cut(s) 439, 821, 2037, 2590, 2790, 2791, 3292, 3346
BssMI GATC 6 cut(s) 669, 1636, 2482, 2641, 3324, 3530
BssNAI GTATAC 1 cut(s) 3317
BssSI CACGAG 1 cut(s) 3099
BssT1I CCWWGG 3 cut(s) 439, 2590, 3292
Bst1107I GTATAC 1 cut(s) 3317
Bst2BI CACGAG 1 cut(s) 3099
Bst2UI CCWGG 6 cut(s) 822, 1445, 2038, 2073, 2097, 2792
Bst4CI ACNGT 6 cut(s) 877, 1239, 2563, 2624, 3263, 3314
Bst6I CTCTTC 8 cut(s) 135, 164, 522, 714, 961, 1104, 1149, 1170
BstAFI CTTAAG 2 cut(s) 2063, 3320
BstAPI GCANNNNNTGC 1 cut(s) 944
BstAUI TGTACA 2 cut(s) 1957, 2932
BstC8I GCNNGC 4 cut(s) 1169, 1262, 1326, 1754
BstDSI CCRYGG 1 cut(s) 2590
BstFNI CGCG 1 cut(s) 3462
BstH2I RGCGCY 1 cut(s) 1165
BstHHI GCGC 2 cut(s) 1164, 3140
BstKTI GATC 6 cut(s) 672, 1639, 2485, 2644, 3327, 3533
BstMAI GTCTC 4 cut(s) 516, 866, 875, 3273
BstMBI GATC 6 cut(s) 669, 1636, 2482, 2641, 3324, 3530
BstNI CCWGG 6 cut(s) 822, 1445, 2038, 2073, 2097, 2792
BstNSI RCATGY 3 cut(s) 1067, 2286, 2831
BstSCI CCNGG 8 cut(s) 820, 1443, 2036, 2071, 2095, 2790, 3345, 3346
BstSFI CTRYAG 7 cut(s) 837, 888, 945, 960, 1007, 2663, 3090
BstUI CGCG 1 cut(s) 3462
BstV2I GAAGAC 4 cut(s) 1334, 1515, 2987, 3223
BstX2I RGATCY 2 cut(s) 2482, 3530
BstYI RGATCY 2 cut(s) 2482, 3530
BstZ17I GTATAC 1 cut(s) 3317
Bsu15I ATCGAT 1 cut(s) 2088
BsuRI GGCC 6 cut(s) 581, 826, 2100, 2589, 2796, 3372
BsuTUI ATCGAT 1 cut(s) 2088
BtgI CCRYGG 1 cut(s) 2590
BtsI GCAGTG 1 cut(s) 2561
BtsIMutI CAGTG 5 cut(s) 156, 1512, 2397, 2561, 3013
BtuMI TCGCGA 1 cut(s) 3462
BveI ACCTGC 3 cut(s) 61, 787, 1733
Cac8I GCNNGC 4 cut(s) 1169, 1262, 1326, 1754
CaiI CAGNNNCTG 4 cut(s) 944, 998, 1324, 1565
CfoI GCGC 2 cut(s) 1164, 3140
Cfr10I RCCGGY 1 cut(s) 3218
Cfr13I GGNCC 8 cut(s) 824, 1562, 2034, 2476, 2669, 2794, 2837, 3304
Cfr9I CCCGGG 1 cut(s) 3346
ClaI ATCGAT 1 cut(s) 2088
CseI GACGC 4 cut(s) 48, 381, 1373, 3331
Csp6I GTAC 7 cut(s) 121, 1958, 2559, 2564, 2736, 2817, 2933
CspCI CAANNNNNGTGG 2 cut(s) 2142, 2177
CviQI GTAC 7 cut(s) 121, 1958, 2559, 2564, 2736, 2817, 2933
DpnI GATC 6 cut(s) 671, 1638, 2484, 2643, 3326, 3532
DpnII GATC 6 cut(s) 669, 1636, 2482, 2641, 3324, 3530
DraIII CACNNNGTG 1 cut(s) 590
DrdI GACNNNNNNGTC 1 cut(s) 1439
DriI GACNNNNNGTC 1 cut(s) 2675
DseDI GACNNNNNNGTC 1 cut(s) 1439
EaeI YGGCCR 2 cut(s) 2098, 3370
Eam1104I CTCTTC 8 cut(s) 135, 164, 522, 714, 961, 1104, 1149, 1170
Eam1105I GACNNNNNGTC 1 cut(s) 2675
EarI CTCTTC 8 cut(s) 135, 164, 522, 714, 961, 1104, 1149, 1170
Eco130I CCWWGG 3 cut(s) 439, 2590, 3292
Eco24I GRGCYC 1 cut(s) 2512
Eco31I GGTCTC 1 cut(s) 3273
Eco32I GATATC 1 cut(s) 3145
Eco47I GGWCC 6 cut(s) 1562, 2034, 2476, 2669, 2837, 3304
Eco57I CTGAAG 4 cut(s) 528, 1308, 1806, 3230
Eco88I CYCGRG 4 cut(s) 27, 230, 3039, 3346
EcoO109I RGGNCCY 4 cut(s) 1562, 2476, 2669, 2794
EcoRI GAATTC 2 cut(s) 2342, 2972
EcoRII CCWGG 6 cut(s) 820, 1443, 2036, 2071, 2095, 2790
EcoRV GATATC 1 cut(s) 3145
EcoT14I CCWWGG 3 cut(s) 439, 2590, 3292
EcoT38I GRGCYC 1 cut(s) 2512
ErhI CCWWGG 3 cut(s) 439, 2590, 3292
Esp3I CGTCTC 1 cut(s) 516
FalI AAGNNNNNCTT 8 cut(s) 766, 798, 1071, 1103, 2952, 2984, 3492, 3524
FaqI GGGAC 7 cut(s) 359, 526, 1371, 2462, 2608, 2682, 3329
FauNDI CATATG 1 cut(s) 2539
FbaI TGATCA 1 cut(s) 2641
FblI GTMKAC 2 cut(s) 2942, 3316
FriOI GRGCYC 1 cut(s) 2512
FspBI CTAG 5 cut(s) 587, 615, 1253, 1532, 2454
GlaI GCGC 2 cut(s) 1163, 3139
GsaI CCCAGC 2 cut(s) 1627, 2990
GsuI CTGGAG 2 cut(s) 1995, 3049
HaeII RGCGCY 1 cut(s) 1165
HaeIII GGCC 6 cut(s) 581, 826, 2100, 2589, 2796, 3372
HapII CCGG 3 cut(s) 552, 3219, 3347
HgaI GACGC 4 cut(s) 48, 381, 1373, 3331
HhaI GCGC 2 cut(s) 1164, 3140
Hin6I GCGC 2 cut(s) 1162, 3138
HinP1I GCGC 2 cut(s) 1162, 3138
HincII GTYRAC 3 cut(s) 2167, 2545, 3409
HindII GTYRAC 3 cut(s) 2167, 2545, 3409
HindIII AAGCTT 1 cut(s) 1262
HpaII CCGG 3 cut(s) 552, 3219, 3347
Hpy99I CGWCG 5 cut(s) 209, 611, 2654, 3314, 3431
HpyCH4III ACNGT 6 cut(s) 877, 1239, 2563, 2624, 3263, 3314
HpyCH4IV ACGT 3 cut(s) 545, 1816, 2939
HpySE526I ACGT 3 cut(s) 545, 1816, 2939
HspAI GCGC 2 cut(s) 1162, 3138
KflI GGGWCCC 1 cut(s) 2476
Ksp22I TGATCA 1 cut(s) 2641
Kzo9I GATC 6 cut(s) 669, 1636, 2482, 2641, 3324, 3530
LguI GCTCTTC 1 cut(s) 1170
LmnI GCTCC 4 cut(s) 1159, 1846, 2299, 2383
MaeI CTAG 5 cut(s) 587, 615, 1253, 1532, 2454
MaeII ACGT 3 cut(s) 545, 1816, 2939
MaeIII GTNAC 6 cut(s) 184, 994, 2332, 2677, 3085, 3515
MalI GATC 6 cut(s) 671, 1638, 2484, 2643, 3326, 3532
MboI GATC 6 cut(s) 669, 1636, 2482, 2641, 3324, 3530
MfeI CAATTG 1 cut(s) 2808
MflI RGATCY 2 cut(s) 2482, 3530
MhlI GDGCHC 1 cut(s) 2512
MlsI TGGCCA 1 cut(s) 2100
MluNI TGGCCA 1 cut(s) 2100
MlyI GAGTC 7 cut(s) 590, 1544, 2114, 2385, 2695, 2876, 3523
MmeI TCCRAC 2 cut(s) 1914, 2815
Mox20I TGGCCA 1 cut(s) 2100
MroXI GAANNNNTTC 1 cut(s) 2342
MscI TGGCCA 1 cut(s) 2100
MslI CAYNNNNRTG 1 cut(s) 2637
Msp20I TGGCCA 1 cut(s) 2100
MspA1I CMGCKG 3 cut(s) 941, 1321, 2990
MspCI CTTAAG 2 cut(s) 2063, 3320
MspI CCGG 3 cut(s) 552, 3219, 3347
MspR9I CCNGG 8 cut(s) 822, 1445, 2038, 2073, 2097, 2792, 3347, 3348
MunI CAATTG 1 cut(s) 2808
Mva1269I GAATGC 3 cut(s) 394, 850, 2296
MvaI CCWGG 6 cut(s) 822, 1445, 2038, 2073, 2097, 2792
MvnI CGCG 1 cut(s) 3462
NciI CCSGG 2 cut(s) 3347, 3348
NcoI CCATGG 1 cut(s) 2590
NdeI CATATG 1 cut(s) 2539
NdeII GATC 6 cut(s) 669, 1636, 2482, 2641, 3324, 3530
NlaIV GGNNCC 5 cut(s) 2070, 2477, 2478, 2670, 3331
NmeAIII GCCGAG 1 cut(s) 42
NmuCI GTSAC 3 cut(s) 2677, 3085, 3515
NruI TCGCGA 1 cut(s) 3462
NspI RCATGY 3 cut(s) 1067, 2286, 2831
PaeR7I CTCGAG 2 cut(s) 27, 3039
PaqCI CACCTGC 1 cut(s) 61
PasI CCCWGGG 1 cut(s) 2791
PciI ACATGT 1 cut(s) 1063
PciSI GCTCTTC 1 cut(s) 1170
PctI GAATGC 3 cut(s) 394, 850, 2296
PdmI GAANNNNTTC 1 cut(s) 2342
PfeI GAWTC 7 cut(s) 80, 335, 989, 1703, 2201, 2888, 3212
PflMI CCANNNNNTGG 1 cut(s) 1324
PleI GAGTC 7 cut(s) 590, 1543, 2113, 2385, 2695, 2875, 3522
PpsI GAGTC 7 cut(s) 590, 1543, 2113, 2385, 2695, 2875, 3522
PpuMI RGGWCCY 3 cut(s) 1562, 2476, 2669
PscI ACATGT 1 cut(s) 1063
PsiI TTATAA 3 cut(s) 368, 1856, 2369
Psp5II RGGWCCY 3 cut(s) 1562, 2476, 2669
Psp6I CCWGG 6 cut(s) 820, 1443, 2036, 2071, 2095, 2790
PspFI CCCAGC 2 cut(s) 1623, 2986
PspGI CCWGG 6 cut(s) 820, 1443, 2036, 2071, 2095, 2790
PspN4I GGNNCC 5 cut(s) 2070, 2477, 2478, 2670, 3331
PspPI GGNCC 8 cut(s) 824, 1562, 2034, 2476, 2669, 2794, 2837, 3304
PspPPI RGGWCCY 3 cut(s) 1562, 2476, 2669
PsrI GAACNNNNNNTAC 2 cut(s) 792, 824
PstI CTGCAG 2 cut(s) 892, 949
PstNI CAGNNNCTG 4 cut(s) 944, 998, 1324, 1565
PsuI RGATCY 2 cut(s) 2482, 3530
PvuII CAGCTG 3 cut(s) 941, 1321, 2990
RruI TCGCGA 1 cut(s) 3462
RsaI GTAC 7 cut(s) 122, 1959, 2560, 2565, 2737, 2818, 2934
RsaNI GTAC 7 cut(s) 121, 1958, 2559, 2564, 2736, 2817, 2933
RseI CAYNNNNRTG 1 cut(s) 2637
SapI GCTCTTC 1 cut(s) 1170
Sau3AI GATC 6 cut(s) 669, 1636, 2482, 2641, 3324, 3530
Sau96I GGNCC 8 cut(s) 824, 1562, 2034, 2476, 2669, 2794, 2837, 3304
ScaI AGTACT 1 cut(s) 122
SchI GAGTC 7 cut(s) 590, 1544, 2114, 2385, 2695, 2876, 3523
ScrFI CCNGG 8 cut(s) 822, 1445, 2038, 2073, 2097, 2792, 3347, 3348
SduI GDGCHC 1 cut(s) 2512
SfcI CTRYAG 7 cut(s) 837, 888, 945, 960, 1007, 2663, 3090
Sfr274I CTCGAG 2 cut(s) 27, 3039
SinI GGWCC 6 cut(s) 1562, 2034, 2476, 2669, 2837, 3304
SlaI CTCGAG 2 cut(s) 27, 3039
SmaI CCCGGG 1 cut(s) 3348
SmiMI CAYNNNNRTG 1 cut(s) 2637
SmlI CTYRAG 6 cut(s) 27, 1181, 2063, 2567, 3039, 3320
SmoI CTYRAG 6 cut(s) 27, 1181, 2063, 2567, 3039, 3320
SpeI ACTAGT 2 cut(s) 614, 2453
SsiI CCGC 7 cut(s) 419, 1096, 1165, 1769, 2051, 2122, 3373
SspMI CTAG 5 cut(s) 587, 615, 1253, 1532, 2454
StyD4I CCNGG 8 cut(s) 820, 1443, 2036, 2071, 2095, 2790, 3345, 3346
StyI CCWWGG 3 cut(s) 439, 2590, 3292
TaaI ACNGT 6 cut(s) 877, 1239, 2563, 2624, 3263, 3314
TaiI ACGT 3 cut(s) 548, 1819, 2942
TaqI TCGA 9 cut(s) 28, 537, 788, 1911, 2088, 2853, 3040, 3524, 3537
TatI WGTACW 5 cut(s) 120, 1957, 2558, 2563, 2932
TauI GCSGC 3 cut(s) 1099, 1167, 3375
TfiI GAWTC 7 cut(s) 80, 335, 989, 1703, 2201, 2888, 3212
TscAI CASTG 5 cut(s) 163, 1519, 2404, 2561, 3013
TseFI GTSAC 3 cut(s) 2677, 3085, 3515
Tsp45I GTSAC 3 cut(s) 2677, 3085, 3515
TspGWI ACGGA 2 cut(s) 338, 1868
TspMI CCCGGG 1 cut(s) 3346
TspRI CASTG 5 cut(s) 163, 1519, 2404, 2561, 3013
Van91I CCANNNNNTGG 1 cut(s) 1324
Vha464I CTTAAG 2 cut(s) 2063, 3320
VpaK11BI GGWCC 6 cut(s) 1562, 2034, 2476, 2669, 2837, 3304
XapI RAATTY 4 cut(s) 1053, 1850, 2342, 2972
XceI RCATGY 3 cut(s) 1067, 2286, 2831
XhoI CTCGAG 2 cut(s) 27, 3039
XmaI CCCGGG 1 cut(s) 3346
XmiI GTMKAC 2 cut(s) 2942, 3316
XmnI GAANNNNTTC 1 cut(s) 2342
XspI CTAG 5 cut(s) 587, 615, 1253, 1532, 2454
ZrmI AGTACT 1 cut(s) 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.