AT1G27000

Protein of unknown function (DUF1664)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003884
Physical Location & Seq
Forward (+)
9373885 .. 9376828
2944 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G27000.1

Sequence Viewer

Length: 915 bp
ATGGCGATGCAGGCCGGAGTTGGCTTATCCAGGATCTTTCTCTTAGCCGGAGCAGGTTACACTGGTACGATCATGATGAAGAATGGAAAATTGTCGGATTTGTTGGGTGAATTGCAGAGTTTGGTGAAGGGAATGGAGAAATCTGGAGAAGGGTCTGAGGGAGATTCTGATGTTTCTGATGCTATAGCTGCACAGGTTCGCCGGTTGGCTATGGAGATTCGACAGCTAGCTTCGCAGCAACACATAACGGTCATGAATGGAGTTTCTGGTGCAAACCTACAAGCCCTTGCTGTTCCTGCTGCGGCATTGGGAGCTCTCGGATATGGTTATATGTGGTGGAAGGGACTCTCATTCACTGACCTTATGTATGTGACAAAAGCCAACATGGCTGCTGCAGTGGCTAACTTGACAAAGAATCTGGAGCAAGTTTCTGAGACTCTTGCGGCTGCGAAAAGGCATTTAACACAAAGGATTCAGAATCTGGATGATAAGGTAGAAAAGCAGATCGATCTCTCGAAGGAAATCAATAGCCAGGTTATTTCTGCTCGTGAGAATATCAGCTCACTTGAGATGGATTTGGAATCATTGCATAATTTGATTACTGGACTGGATGGGAAGTTGGACACGCTGGAATACAAGCAGGATGTCACAAATGTTTTCATGCTAAACTTGTATAACTATTTTGGAGGCAAGAGCACAAAGCTGCCTGAAATGGAACAGCTTCAGCTTCCTGTAAACCAGAGGGCTCGTAATTTGCTTGCAGATGTTGAAACTAAGGGTCTGAAAAACTTGGCTGAAGAGCTGTTTAAAAGCAATGGCACACAGGTGACAACCACAGTGAAACAAATCAGTTTGAGTAAAGTGAATGTGAAGTCGAGGCCATTGTTATCAAGGGCTGCTTCAGCTAAGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1664

Protein Analysis

304

Amino Acids

33.01

Weight (kDa)

8.46

Isoelectric Point (pI)

30.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1664 PF07889 92 - 214 1.4e-46 Protein of unknown function (DUF1664)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 44
AciI CCGC 2 cut(s) 302, 443
AclWI GGATC 1 cut(s) 41
AcuI CTGAAG 3 cut(s) 707, 816, 885
AfaI GTAC 1 cut(s) 67
AgsI TTSAA 1 cut(s) 770
AjnI CCWGG 2 cut(s) 29, 531
AleI CACNNNNGTG 1 cut(s) 824
Alw21I GWGCWC 2 cut(s) 316, 698
Alw26I GTCTC 1 cut(s) 428
AlwI GGATC 1 cut(s) 41
AlwNI CAGNNNCTG 1 cut(s) 481
AoxI GGCC 2 cut(s) 12, 878
ApeKI GCWGC 8 cut(s) 188, 235, 299, 389, 392, 446, 703, 896
Asp700I GAANNNNTTC 1 cut(s) 720
AsuHPI GGTGA 3 cut(s) 119, 136, 838
AsuNHI GCTAGC 1 cut(s) 226
BanII GRGCYC 2 cut(s) 316, 748
BauI CACGAG 1 cut(s) 546
Bbv12I GWGCWC 2 cut(s) 316, 698
BbvI GCAGC 8 cut(s) 175, 247, 286, 376, 379, 433, 690, 883
BccI CCATC 2 cut(s) 565, 605
BciT130I CCWGG 2 cut(s) 31, 533
BcoDI GTCTC 1 cut(s) 428
BfaI CTAG 1 cut(s) 227
BfmI CTRYAG 2 cut(s) 183, 393
BfuAI ACCTGC 1 cut(s) 44
BglI GCCNNNNNGGC 1 cut(s) 386
Bme1390I CCNGG 2 cut(s) 31, 533
BmrFI CCNGG 2 cut(s) 31, 533
BmsI GCATC 1 cut(s) 169
BmtI GCTAGC 1 cut(s) 230
BpmI CTGGAG 2 cut(s) 165, 440
BpuEI CTTGAG 1 cut(s) 587
Bsa29I ATCGAT 1 cut(s) 507
Bse118I RCCGGY 1 cut(s) 201
Bse1I ACTGG 3 cut(s) 67, 607, 612
Bse3DI GCAATG 2 cut(s) 584, 820
BseBI CCWGG 2 cut(s) 31, 533
BseCI ATCGAT 1 cut(s) 507
BseGI GGATG 3 cut(s) 490, 616, 649
BseMI GCAATG 2 cut(s) 584, 820
BseMII CTCAG 2 cut(s) 147, 423
BseNI ACTGG 3 cut(s) 67, 607, 612
BseXI GCAGC 8 cut(s) 175, 247, 286, 376, 379, 433, 690, 883
BsgI GTGCAG 1 cut(s) 174
BshFI GGCC 2 cut(s) 14, 880
BshVI ATCGAT 1 cut(s) 507
BsiHKAI GWGCWC 2 cut(s) 316, 698
BsiSI CCGG 3 cut(s) 15, 48, 202
BslFI GGGAC 1 cut(s) 357
BsmAI GTCTC 1 cut(s) 428
BsmFI GGGAC 1 cut(s) 357
BsnI GGCC 2 cut(s) 14, 880
Bsp1286I GDGCHC 3 cut(s) 316, 698, 748
Bsp143I GATC 4 cut(s) 33, 69, 504, 508
BspACI CCGC 2 cut(s) 302, 443
BspANI GGCC 2 cut(s) 14, 880
BspCNI CTCAG 2 cut(s) 148, 424
BspDI ATCGAT 1 cut(s) 507
BspHI TCATGA 2 cut(s) 72, 252
BspMAI CTGCAG 1 cut(s) 397
BspMI ACCTGC 1 cut(s) 44
BspOI GCTAGC 1 cut(s) 230
BspPI GGATC 1 cut(s) 41
BspQI GCTCTTC 1 cut(s) 792
BsrDI GCAATG 2 cut(s) 584, 820
BsrFI RCCGGY 1 cut(s) 201
BsrI ACTGG 3 cut(s) 67, 607, 612
BssAI RCCGGY 1 cut(s) 201
BssMI GATC 4 cut(s) 33, 69, 504, 508
BssSI CACGAG 1 cut(s) 546
Bst2BI CACGAG 1 cut(s) 546
Bst2UI CCWGG 2 cut(s) 31, 533
Bst4CI ACNGT 2 cut(s) 250, 838
Bst6I CTCTTC 1 cut(s) 792
BstC8I GCNNGC 3 cut(s) 12, 228, 759
BstDEI CTNAG 5 cut(s) 43, 156, 432, 774, 906
BstF5I GGATG 3 cut(s) 490, 616, 649
BstKTI GATC 4 cut(s) 36, 72, 507, 511
BstMAI GTCTC 1 cut(s) 428
BstMBI GATC 4 cut(s) 33, 69, 504, 508
BstMWI GCNNNNNNNGC 8 cut(s) 11, 188, 232, 296, 311, 386, 398, 902
BstNI CCWGG 2 cut(s) 31, 533
BstSCI CCNGG 2 cut(s) 29, 531
BstSFI CTRYAG 2 cut(s) 183, 393
BstV1I GCAGC 8 cut(s) 175, 247, 286, 376, 379, 433, 690, 883
BstX2I RGATCY 1 cut(s) 33
BstYI RGATCY 1 cut(s) 33
Bsu15I ATCGAT 1 cut(s) 507
BsuRI GGCC 2 cut(s) 14, 880
BsuTUI ATCGAT 1 cut(s) 507
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 3 cut(s) 490, 616, 649
BtsI GCAGTG 1 cut(s) 402
BtsIMutI CAGTG 4 cut(s) 60, 354, 402, 843
BveI ACCTGC 1 cut(s) 44
Cac8I GCNNGC 3 cut(s) 12, 228, 759
CaiI CAGNNNCTG 1 cut(s) 481
CciI TCATGA 2 cut(s) 72, 252
Cfr10I RCCGGY 1 cut(s) 201
ClaI ATCGAT 1 cut(s) 507
Csp6I GTAC 1 cut(s) 66
CviAII CATG 4 cut(s) 73, 253, 385, 661
CviQI GTAC 1 cut(s) 66
DdeI CTNAG 5 cut(s) 43, 156, 432, 774, 906
DpnI GATC 4 cut(s) 35, 71, 506, 510
DpnII GATC 4 cut(s) 33, 69, 504, 508
DraI TTTAAA 1 cut(s) 808
Eam1104I CTCTTC 1 cut(s) 792
EarI CTCTTC 1 cut(s) 792
Ecl136II GAGCTC 1 cut(s) 314
Eco24I GRGCYC 2 cut(s) 316, 748
Eco53kI GAGCTC 1 cut(s) 314
Eco57I CTGAAG 3 cut(s) 707, 816, 885
EcoICRI GAGCTC 1 cut(s) 314
EcoRII CCWGG 2 cut(s) 29, 531
EcoT38I GRGCYC 2 cut(s) 316, 748
FaeI CATG 4 cut(s) 76, 256, 388, 664
FalI AAGNNNNNCTT 2 cut(s) 883, 915
FaqI GGGAC 1 cut(s) 357
FatI CATG 4 cut(s) 72, 252, 384, 660
FokI GGATG 3 cut(s) 497, 623, 656
FriOI GRGCYC 2 cut(s) 316, 748
FspBI CTAG 1 cut(s) 227
GsuI CTGGAG 2 cut(s) 165, 440
HaeIII GGCC 2 cut(s) 14, 880
HapII CCGG 3 cut(s) 15, 48, 202
Hin1II CATG 4 cut(s) 76, 256, 388, 664
HinfI GANTC 8 cut(s) 164, 217, 345, 415, 436, 472, 478, 581
HpaII CCGG 3 cut(s) 15, 48, 202
HphI GGTGA 3 cut(s) 119, 136, 838
Hpy166II GTNNAC 1 cut(s) 736
Hpy188I TCNGA 8 cut(s) 97, 157, 169, 178, 320, 433, 477, 783
Hpy188III TCNNGA 7 cut(s) 73, 144, 253, 419, 482, 514, 548
Hpy8I GTNNAC 1 cut(s) 736
HpyAV CCTTC 4 cut(s) 121, 143, 334, 511
HpyCH4III ACNGT 2 cut(s) 250, 838
HpyCH4V TGCA 7 cut(s) 10, 115, 191, 272, 395, 589, 761
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 188, 232, 296, 311, 386, 398, 902
HpyF3I CTNAG 5 cut(s) 43, 156, 432, 774, 906
Hsp92II CATG 4 cut(s) 76, 256, 388, 664
Kzo9I GATC 4 cut(s) 33, 69, 504, 508
LguI GCTCTTC 1 cut(s) 792
LmnI GCTCC 3 cut(s) 50, 311, 421
Lsp1109I GCAGC 8 cut(s) 175, 247, 286, 376, 379, 433, 690, 883
LweI GCATC 1 cut(s) 169
MaeI CTAG 1 cut(s) 227
MaeIII GTNAC 4 cut(s) 56, 370, 646, 826
MalI GATC 4 cut(s) 35, 71, 506, 510
MboI GATC 4 cut(s) 33, 69, 504, 508
MboII GAAGA 2 cut(s) 91, 809
MflI RGATCY 1 cut(s) 33
MhlI GDGCHC 3 cut(s) 316, 698, 748
MluCI AATT 4 cut(s) 89, 110, 592, 751
MlyI GAGTC 2 cut(s) 339, 430
MmeI TCCRAC 2 cut(s) 75, 600
MnlI CCTC 4 cut(s) 151, 680, 735, 870
MroXI GAANNNNTTC 1 cut(s) 720
MseI TTAA 2 cut(s) 461, 807
MslI CAYNNNNRTG 1 cut(s) 824
MspI CCGG 3 cut(s) 15, 48, 202
MspR9I CCNGG 2 cut(s) 31, 533
MvaI CCWGG 2 cut(s) 31, 533
MwoI GCNNNNNNNGC 8 cut(s) 11, 188, 232, 296, 311, 386, 398, 902
NdeII GATC 4 cut(s) 33, 69, 504, 508
NheI GCTAGC 1 cut(s) 226
NlaIII CATG 4 cut(s) 76, 256, 388, 664
NmuCI GTSAC 3 cut(s) 370, 646, 826
OliI CACNNNNGTG 1 cut(s) 824
PagI TCATGA 2 cut(s) 72, 252
PciSI GCTCTTC 1 cut(s) 792
PdmI GAANNNNTTC 1 cut(s) 720
PfeI GAWTC 6 cut(s) 164, 217, 415, 472, 478, 581
PfoI TCCNGGA 1 cut(s) 29
PleI GAGTC 2 cut(s) 339, 430
PpsI GAGTC 2 cut(s) 339, 430
Psp124BI GAGCTC 1 cut(s) 316
Psp6I CCWGG 2 cut(s) 29, 531
PspGI CCWGG 2 cut(s) 29, 531
PstI CTGCAG 1 cut(s) 397
PstNI CAGNNNCTG 1 cut(s) 481
PsuI RGATCY 1 cut(s) 33
RsaI GTAC 1 cut(s) 67
RsaNI GTAC 1 cut(s) 66
RseI CAYNNNNRTG 1 cut(s) 824
SacI GAGCTC 1 cut(s) 316
SapI GCTCTTC 1 cut(s) 792
SaqAI TTAA 2 cut(s) 461, 807
Sau3AI GATC 4 cut(s) 33, 69, 504, 508
SchI GAGTC 2 cut(s) 339, 430
ScrFI CCNGG 2 cut(s) 31, 533
SduI GDGCHC 3 cut(s) 316, 698, 748
SfaNI GCATC 1 cut(s) 169
SfcI CTRYAG 2 cut(s) 183, 393
SmiMI CAYNNNNRTG 1 cut(s) 824
SmlI CTYRAG 1 cut(s) 566
SmoI CTYRAG 1 cut(s) 566
Sse9I AATT 4 cut(s) 89, 110, 592, 751
SsiI CCGC 2 cut(s) 302, 443
SspMI CTAG 1 cut(s) 227
SstI GAGCTC 1 cut(s) 316
StyD4I CCNGG 2 cut(s) 29, 531
TaaI ACNGT 2 cut(s) 250, 838
TaqI TCGA 4 cut(s) 220, 507, 515, 875
TasI AATT 4 cut(s) 89, 110, 592, 751
TauI GCSGC 2 cut(s) 305, 446
TfiI GAWTC 6 cut(s) 164, 217, 415, 472, 478, 581
Tru1I TTAA 2 cut(s) 461, 807
Tru9I TTAA 2 cut(s) 461, 807
TscAI CASTG 4 cut(s) 67, 361, 402, 843
TseFI GTSAC 3 cut(s) 370, 646, 826
TseI GCWGC 8 cut(s) 188, 235, 299, 389, 392, 446, 703, 896
Tsp45I GTSAC 3 cut(s) 370, 646, 826
TspDTI ATGAA 3 cut(s) 92, 269, 649
TspRI CASTG 4 cut(s) 67, 361, 402, 843
XmnI GAANNNNTTC 1 cut(s) 720
XspI CTAG 1 cut(s) 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.