MD16G1077900.v1.1

Protein of unknown function (DUF1664)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0003884
Physical Location & Seq
Forward (+)
5458399 .. 5462801
4403 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1077900.v1.1.491

Sequence Viewer

Length: 918 bp
ATGGCTATGCAGAGCGGAATAGGGTTTTCCAAGATCCTGATCTTAGCAGGGGCAGGGTATACAAGCACCATCCTGCTTAAGAACGGTAAATTATCCGACTTGCTCGGTGAACTTCAGTCTTTGTTGAATGGATCCGGGGAACACACAGAAGGTGACTATGATTCCTTGACAGCACAGATTAGCCGACTAACAGCTGAGATCAGGCAAATTGGCTCGTCGCGGGGCGGCAGCACGGTTTTCGTGAATGGGAACGGTAGCCAAATTGGTAATATGACATCTCTAATAATGCCAGCAGCTACCTTGGGAGCAGTGGGTTATGGTTACATGTGGTGGAAGGGTCTAAAACTCTCAGATATTATGTATGTGACAAAACGTGGCATGGCTACCGCTGTTGAGAACTTGACTAAAAATCTGGATAATGTGAAAGAGGCCGTTGCTAAAGCAAAGAAGCACCTGACGCAGAGAATCCAGCATGTTGATGACAAAATGGTGGAGCAAAATGAGCTCTCAAGGTCAATTAAGGAAGATGTTGCTGGAGTGCAACATTCTCTCACTGATATTGACTATGACTTGAGTAGATTGCAGAGCATGGTTTCTGGTTTGGATCATAAGATTGGTTCTCTTGAACACAAGCAGGATCTCGCAAACCTTGGTGTTATCTACCTGGTCAATTTTGTGGATGGGAAAAAAGTTGAAATGCCTAAAACTCTGCAGAAGCAACTTGAAAACCCTGGGAAGTCACGTGGTCGTTTACTCTCATATTCAGATAGTTCAGGCTTGATGGGTCTCAAAGAAATTGCGGACTCTCTGTCTGAAACATTAAATCCATCAACTGATGCTATTGTGAAAGATGATATTGAAATGACGGGGGAGTATCGGAAGAACAACCTGATAAGGTCGGTGTCGACCAGATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1664

Protein Analysis

306

Amino Acids

33.2

Weight (kDa)

7.03

Isoelectric Point (pI)

34.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1664 PF07889 89 - 212 7.8e-48 Protein of unknown function (DUF1664)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 15
AccI GTMKAC 2 cut(s) 59, 905
AccII CGCG 1 cut(s) 220
AciI CCGC 5 cut(s) 15, 220, 225, 387, 800
AclWI GGATC 5 cut(s) 28, 126, 139, 612, 645
AcuI CTGAAG 1 cut(s) 98
AcvI CACGTG 1 cut(s) 743
AflII CTTAAG 1 cut(s) 77
AflIII ACRYGT 1 cut(s) 324
AgsI TTSAA 5 cut(s) 127, 626, 695, 725, 860
AhdI GACNNNNNGTC 1 cut(s) 808
AjnI CCWGG 2 cut(s) 663, 730
AluBI AGCT 3 cut(s) 194, 296, 505
AluI AGCT 3 cut(s) 194, 296, 505
Alw21I GWGCWC 1 cut(s) 507
Alw26I GTCTC 1 cut(s) 791
AlwI GGATC 5 cut(s) 28, 126, 139, 612, 645
AoxI GGCC 1 cut(s) 429
ApeKI GCWGC 2 cut(s) 228, 293
AsuC2I CCSGG 1 cut(s) 136
AsuHPI GGTGA 2 cut(s) 119, 164
BamHI GGATCC 1 cut(s) 131
BanII GRGCYC 1 cut(s) 507
BbrPI CACGTG 1 cut(s) 743
Bbv12I GWGCWC 1 cut(s) 507
BbvI GCAGC 2 cut(s) 240, 305
BccI CCATC 4 cut(s) 77, 674, 775, 835
BceAI ACGGC 1 cut(s) 416
BcgI CGANNNNNNTGC 2 cut(s) 220, 254
BciT130I CCWGG 2 cut(s) 665, 732
BcnI CCSGG 1 cut(s) 136
BcoDI GTCTC 1 cut(s) 791
BfmI CTRYAG 1 cut(s) 710
BfrI CTTAAG 1 cut(s) 77
BisI GCNGC 3 cut(s) 226, 229, 294
BlsI GCNGC 3 cut(s) 227, 230, 295
Bme1390I CCNGG 3 cut(s) 136, 665, 732
BmeRI GACNNNNNGTC 1 cut(s) 808
BmiI GGNNCC 1 cut(s) 133
BmrFI CCNGG 3 cut(s) 136, 665, 732
BmsI GCATC 1 cut(s) 826
BpmI CTGGAG 1 cut(s) 555
BpuEI CTTGAG 2 cut(s) 493, 592
BpuMI CCSGG 1 cut(s) 136
BsaAI YACGTR 1 cut(s) 743
BsaBI GATNNNNATC 1 cut(s) 38
BsaI GGTCTC 1 cut(s) 791
BsaJI CCNNGG 5 cut(s) 135, 300, 649, 730, 731
Bse8I GATNNNNATC 1 cut(s) 38
BseBI CCWGG 2 cut(s) 665, 732
BseDI CCNNGG 5 cut(s) 135, 300, 649, 730, 731
BseGI GGATG 2 cut(s) 69, 685
BseJI GATNNNNATC 1 cut(s) 38
BseMII CTCAG 2 cut(s) 186, 363
BseXI GCAGC 2 cut(s) 240, 305
Bsh1236I CGCG 1 cut(s) 220
BshFI GGCC 1 cut(s) 431
BsiHKAI GWGCWC 1 cut(s) 507
BsiSI CCGG 1 cut(s) 135
BsmAI GTCTC 1 cut(s) 791
BsnI GGCC 1 cut(s) 431
Bso31I GGTCTC 1 cut(s) 791
Bsp1286I GDGCHC 1 cut(s) 507
Bsp143I GATC 6 cut(s) 33, 39, 131, 198, 604, 637
BspACI CCGC 5 cut(s) 15, 220, 225, 387, 800
BspANI GGCC 1 cut(s) 431
BspCNI CTCAG 2 cut(s) 187, 362
BspFNI CGCG 1 cut(s) 220
BspLI GGNNCC 1 cut(s) 133
BspMAI CTGCAG 1 cut(s) 714
BspPI GGATC 5 cut(s) 28, 126, 139, 612, 645
BspTI CTTAAG 1 cut(s) 77
BspTNI GGTCTC 1 cut(s) 791
BsrBI CCGCTC 1 cut(s) 15
BssECI CCNNGG 5 cut(s) 135, 300, 649, 730, 731
BssMI GATC 6 cut(s) 33, 39, 131, 198, 604, 637
BssNAI GTATAC 1 cut(s) 60
BssT1I CCWWGG 2 cut(s) 300, 649
Bst1107I GTATAC 1 cut(s) 60
Bst2UI CCWGG 2 cut(s) 665, 732
Bst4CI ACNGT 3 cut(s) 86, 235, 254
BstAFI CTTAAG 1 cut(s) 77
BstBAI YACGTR 1 cut(s) 743
BstC8I GCNNGC 1 cut(s) 291
BstDEI CTNAG 3 cut(s) 43, 195, 349
BstF5I GGATG 2 cut(s) 69, 685
BstFNI CGCG 1 cut(s) 220
BstKTI GATC 6 cut(s) 36, 42, 134, 201, 607, 640
BstMAI GTCTC 1 cut(s) 791
BstMBI GATC 6 cut(s) 33, 39, 131, 198, 604, 637
BstMWI GCNNNNNNNGC 2 cut(s) 457, 502
BstNI CCWGG 2 cut(s) 665, 732
BstNSI RCATGY 2 cut(s) 328, 476
BstSCI CCNGG 3 cut(s) 134, 663, 730
BstSFI CTRYAG 1 cut(s) 710
BstUI CGCG 1 cut(s) 220
BstV1I GCAGC 2 cut(s) 240, 305
BstX2I RGATCY 3 cut(s) 33, 131, 637
BstYI RGATCY 3 cut(s) 33, 131, 637
BstZ17I GTATAC 1 cut(s) 60
BsuRI GGCC 1 cut(s) 431
BtsCI GGATG 2 cut(s) 69, 685
BtsI GCAGTG 1 cut(s) 315
BtsIMutI CAGTG 2 cut(s) 315, 552
Cac8I GCNNGC 1 cut(s) 291
CseI GACGC 1 cut(s) 466
CsiI ACCWGGT 1 cut(s) 663
CviAII CATG 4 cut(s) 325, 379, 473, 589
DdeI CTNAG 3 cut(s) 43, 195, 349
DpnI GATC 6 cut(s) 35, 41, 133, 200, 606, 639
DpnII GATC 6 cut(s) 33, 39, 131, 198, 604, 637
DriI GACNNNNNGTC 1 cut(s) 808
Eam1105I GACNNNNNGTC 1 cut(s) 808
Ecl136II GAGCTC 1 cut(s) 505
Eco130I CCWWGG 2 cut(s) 300, 649
Eco24I GRGCYC 1 cut(s) 507
Eco31I GGTCTC 1 cut(s) 791
Eco53kI GAGCTC 1 cut(s) 505
Eco57I CTGAAG 1 cut(s) 98
Eco72I CACGTG 1 cut(s) 743
EcoICRI GAGCTC 1 cut(s) 505
EcoRII CCWGG 2 cut(s) 663, 730
EcoT14I CCWWGG 2 cut(s) 300, 649
EcoT38I GRGCYC 1 cut(s) 507
ErhI CCWWGG 2 cut(s) 300, 649
FaeI CATG 4 cut(s) 328, 382, 476, 592
FatI CATG 4 cut(s) 324, 378, 472, 588
FauI CCCGC 1 cut(s) 213
FblI GTMKAC 2 cut(s) 59, 905
Fnu4HI GCNGC 3 cut(s) 226, 229, 294
FokI GGATG 2 cut(s) 56, 692
FriOI GRGCYC 1 cut(s) 507
Fsp4HI GCNGC 3 cut(s) 226, 229, 294
GluI GCNGC 3 cut(s) 226, 229, 294
GsuI CTGGAG 1 cut(s) 555
HaeIII GGCC 1 cut(s) 431
HapII CCGG 1 cut(s) 135
HgaI GACGC 1 cut(s) 466
Hin1II CATG 4 cut(s) 328, 382, 476, 592
HincII GTYRAC 1 cut(s) 906
HindII GTYRAC 1 cut(s) 906
HinfI GANTC 3 cut(s) 161, 465, 803
HpaII CCGG 1 cut(s) 135
HphI GGTGA 2 cut(s) 119, 164
Hpy166II GTNNAC 4 cut(s) 60, 110, 752, 906
Hpy188I TCNGA 5 cut(s) 97, 352, 766, 814, 879
Hpy188III TCNNGA 4 cut(s) 37, 241, 413, 623
Hpy8I GTNNAC 4 cut(s) 60, 110, 752, 906
Hpy99I CGWCG 1 cut(s) 220
HpyAV CCTTC 2 cut(s) 143, 328
HpyCH4III ACNGT 3 cut(s) 86, 235, 254
HpyCH4IV ACGT 2 cut(s) 373, 742
HpyCH4V TGCA 4 cut(s) 10, 541, 583, 712
HpyF10VI GCNNNNNNNGC 2 cut(s) 457, 502
HpyF3I CTNAG 3 cut(s) 43, 195, 349
HpySE526I ACGT 2 cut(s) 373, 742
Hsp92II CATG 4 cut(s) 328, 382, 476, 592
Kzo9I GATC 6 cut(s) 33, 39, 131, 198, 604, 637
LmnI GCTCC 2 cut(s) 305, 493
Lsp1109I GCAGC 2 cut(s) 240, 305
LweI GCATC 1 cut(s) 826
MabI ACCWGGT 1 cut(s) 663
MaeII ACGT 2 cut(s) 373, 742
MaeIII GTNAC 4 cut(s) 152, 320, 364, 738
MalI GATC 6 cut(s) 35, 41, 133, 200, 606, 639
MbiI CCGCTC 1 cut(s) 15
MboI GATC 6 cut(s) 33, 39, 131, 198, 604, 637
MboII GAAGA 2 cut(s) 536, 892
MflI RGATCY 3 cut(s) 33, 131, 637
MhlI GDGCHC 1 cut(s) 507
MluCI AATT 6 cut(s) 89, 207, 261, 516, 670, 795
MlyI GAGTC 1 cut(s) 797
MmeI TCCRAC 1 cut(s) 120
MnlI CCTC 1 cut(s) 421
MseI TTAA 3 cut(s) 78, 519, 821
MslI CAYNNNNRTG 1 cut(s) 477
MspA1I CMGCKG 2 cut(s) 194, 389
MspCI CTTAAG 1 cut(s) 77
MspI CCGG 1 cut(s) 135
MspR9I CCNGG 3 cut(s) 136, 665, 732
MvaI CCWGG 2 cut(s) 665, 732
MvnI CGCG 1 cut(s) 220
MwoI GCNNNNNNNGC 2 cut(s) 457, 502
NciI CCSGG 1 cut(s) 136
NdeII GATC 6 cut(s) 33, 39, 131, 198, 604, 637
NlaIII CATG 4 cut(s) 328, 382, 476, 592
NlaIV GGNNCC 1 cut(s) 133
NmuCI GTSAC 3 cut(s) 152, 364, 738
NspI RCATGY 2 cut(s) 328, 476
PasI CCCWGGG 1 cut(s) 731
PciI ACATGT 1 cut(s) 324
PfeI GAWTC 2 cut(s) 161, 465
PkrI GCNGC 3 cut(s) 227, 230, 295
PleI GAGTC 1 cut(s) 797
PmaCI CACGTG 1 cut(s) 743
PmlI CACGTG 1 cut(s) 743
PpsI GAGTC 1 cut(s) 797
Ppu21I YACGTR 1 cut(s) 743
PscI ACATGT 1 cut(s) 324
Psp124BI GAGCTC 1 cut(s) 507
Psp6I CCWGG 2 cut(s) 663, 730
PspCI CACGTG 1 cut(s) 743
PspGI CCWGG 2 cut(s) 663, 730
PspN4I GGNNCC 1 cut(s) 133
PstI CTGCAG 1 cut(s) 714
PsuI RGATCY 3 cut(s) 33, 131, 637
PvuII CAGCTG 1 cut(s) 194
RseI CAYNNNNRTG 1 cut(s) 477
SacI GAGCTC 1 cut(s) 507
SalI GTCGAC 1 cut(s) 904
SaqAI TTAA 3 cut(s) 78, 519, 821
SatI GCNGC 3 cut(s) 226, 229, 294
Sau3AI GATC 6 cut(s) 33, 39, 131, 198, 604, 637
SchI GAGTC 1 cut(s) 797
ScrFI CCNGG 3 cut(s) 136, 665, 732
SduI GDGCHC 1 cut(s) 507
SexAI ACCWGGT 1 cut(s) 663
SfaNI GCATC 1 cut(s) 826
SfcI CTRYAG 1 cut(s) 710
SmiMI CAYNNNNRTG 1 cut(s) 477
SmlI CTYRAG 3 cut(s) 77, 508, 571
SmoI CTYRAG 3 cut(s) 77, 508, 571
Sse9I AATT 6 cut(s) 89, 207, 261, 516, 670, 795
SsiI CCGC 5 cut(s) 15, 220, 225, 387, 800
SstI GAGCTC 1 cut(s) 507
StyD4I CCNGG 3 cut(s) 134, 663, 730
StyI CCWWGG 2 cut(s) 300, 649
TaaI ACNGT 3 cut(s) 86, 235, 254
TaiI ACGT 2 cut(s) 376, 745
TaqI TCGA 1 cut(s) 905
TasI AATT 6 cut(s) 89, 207, 261, 516, 670, 795
TauI GCSGC 1 cut(s) 228
TfiI GAWTC 2 cut(s) 161, 465
Tru1I TTAA 3 cut(s) 78, 519, 821
Tru9I TTAA 3 cut(s) 78, 519, 821
TscAI CASTG 2 cut(s) 315, 559
TseFI GTSAC 3 cut(s) 152, 364, 738
TseI GCWGC 2 cut(s) 228, 293
Tsp45I GTSAC 3 cut(s) 152, 364, 738
TspRI CASTG 2 cut(s) 315, 559
Vha464I CTTAAG 1 cut(s) 77
XceI RCATGY 2 cut(s) 328, 476
XmiI GTMKAC 2 cut(s) 59, 905
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.