FvH4_4g31870

Protein of unknown function (DUF1664)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0003884
Physical Location & Seq
Forward (+)
30798862 .. 30802665
3804 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g31870.t1

Sequence Viewer

Length: 882 bp
ATGGCTCAGATAGGTCTCACCAGGGTCATCCTCATCGCCGGAGCAGGTTACACTACCTCCATCCTCGCTCGGAACGGCAAGCTCTCCGACGTGCTCGCTGAACTTCAGGCTTTGTTGAAAGGATCAGGAGAGCATGAAGGAGATTCTGATAAAGTTGCGTCGCAGATGTATAACCGATTGCTGGATGAGATACGCACTGCACGACAAACTGTTGTTCTGAATGCCAATGCTGGCCAGGGTAGCTTGTCATTTCTTATAATGCCAGCTGCTACACTGGGGGCAGTGGGATATGCTTACATGTGGTGGAAGGGCCTTAAATTCTCGGATCTCATGTATGTGACAAAGCGTAATATGACTGCTGCTGTTACAAACCTGACCAAAAGTCTGGATACTGTAAAAGAAGCTGTTGTTAATACAAAGAAGCACCTGACGCAGAGGATCCAGAATTTGGATGATAAAGTGCTGGAACAAAATGAACTCTCAAAGACAATTAAGAATGATGTTGCTGGGGTGCAATATTCTCTGACTGAAATGGACTTTACGTTGAGTCAGCTGCAGTGTTTGGTTGCTGGTCTGGATGGGAAGATTTGTTCTCTTGAAGAAAAGCAGGATTTCGCAAATATGGGTGTTGCTTACCTCTGCAACTTTGTTAGTGGGAAAAAAATTGAAATGCCTGGAGCTCTGCAGAAGCAACTAAAACTTTCTGATAATGGACGGGGTAGCCGATTGCTCTCATATTCAGAACCAAACGTGATGGGGCTTAAAGAAATTGCGGAATCTTTGACTTCCCGTTTGAGTACTGATGGTATCGTGCAAGATGATATTACCAAGTTAGAAGAGCCTCCAAAGAACTTGCTGAGGTCGGTGTCAACCAAGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1664

Protein Analysis

294

Amino Acids

32.02

Weight (kDa)

8.22

Isoelectric Point (pI)

30.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1664 PF07889 80 - 203 7e-45 Protein of unknown function (DUF1664)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 257
Acc36I ACCTGC 1 cut(s) 35
AccB7I CCANNNNNTGG 1 cut(s) 448
AciI CCGC 1 cut(s) 773
AclWI GGATC 4 cut(s) 130, 333, 433, 446
AcoI YGGCCR 1 cut(s) 232
AcsI RAATTY 2 cut(s) 317, 445
AcuI CTGAAG 1 cut(s) 89
AfaI GTAC 1 cut(s) 799
AfiI CCNNNNNNNGG 2 cut(s) 181, 448
AflIII ACRYGT 1 cut(s) 297
AgsI TTSAA 3 cut(s) 118, 599, 668
AhdI GACNNNNNGTC 1 cut(s) 381
AjiI CACGTC 1 cut(s) 91
AjnI CCWGG 3 cut(s) 20, 234, 673
AloI GAACNNNNNNTCC 2 cut(s) 574, 606
AluBI AGCT 6 cut(s) 82, 243, 266, 404, 553, 680
AluI AGCT 6 cut(s) 82, 243, 266, 404, 553, 680
Alw21I GWGCWC 2 cut(s) 96, 682
Alw26I GTCTC 1 cut(s) 20
AlwI GGATC 4 cut(s) 130, 333, 433, 446
AoxI GGCC 2 cut(s) 232, 310
ApeKI GCWGC 3 cut(s) 266, 359, 553
ApoI RAATTY 2 cut(s) 317, 445
ArsI GACNNNNNNTTYG 2 cut(s) 775, 807
AspS9I GGNCC 1 cut(s) 310
AsuHPI GGTGA 1 cut(s) 10
BalI TGGCCA 1 cut(s) 234
BamHI GGATCC 1 cut(s) 438
BanII GRGCYC 1 cut(s) 682
Bbv12I GWGCWC 2 cut(s) 96, 682
BbvCI CCTCAGC 1 cut(s) 857
BbvI GCAGC 3 cut(s) 253, 346, 540
BccI CCATC 4 cut(s) 68, 572, 748, 797
BceAI ACGGC 1 cut(s) 91
BciT130I CCWGG 3 cut(s) 22, 236, 675
BciVI GTATCC 1 cut(s) 382
BcoDI GTCTC 1 cut(s) 20
BfmI CTRYAG 2 cut(s) 554, 683
BfuAI ACCTGC 1 cut(s) 35
BfuI GTATCC 1 cut(s) 382
BisI GCNGC 3 cut(s) 267, 360, 554
BlsI GCNGC 3 cut(s) 268, 361, 555
BmcAI AGTACT 1 cut(s) 799
Bme1390I CCNGG 3 cut(s) 22, 236, 675
BmeRI GACNNNNNGTC 1 cut(s) 381
BmgBI CACGTC 1 cut(s) 91
BmgT120I GGNCC 1 cut(s) 310
BmiI GGNNCC 1 cut(s) 440
BmrFI CCNGG 3 cut(s) 22, 236, 675
BmrI ACTGGG 1 cut(s) 284
BmuI ACTGGG 1 cut(s) 284
BpmI CTGGAG 1 cut(s) 696
Bpu10I CCTNAGC 1 cut(s) 857
BsaI GGTCTC 1 cut(s) 20
BsaJI CCNNGG 2 cut(s) 21, 235
BsaXI ACNNNNNCTCC 2 cut(s) 41, 71
Bsc4I CCNNNNNNNGG 2 cut(s) 181, 448
Bse1I ACTGG 1 cut(s) 279
BseBI CCWGG 3 cut(s) 22, 236, 675
BseDI CCNNGG 2 cut(s) 21, 235
BseGI GGATG 5 cut(s) 27, 60, 190, 457, 583
BseLI CCNNNNNNNGG 2 cut(s) 181, 448
BseMII CTCAG 2 cut(s) 20, 848
BseNI ACTGG 1 cut(s) 279
BseXI GCAGC 3 cut(s) 253, 346, 540
BseYI CCCAGC 1 cut(s) 506
BsgI GTGCAG 1 cut(s) 183
BshFI GGCC 2 cut(s) 234, 312
BsiHKAI GWGCWC 2 cut(s) 96, 682
BsiSI CCGG 1 cut(s) 39
BslI CCNNNNNNNGG 2 cut(s) 181, 448
BsmAI GTCTC 1 cut(s) 20
BsmI GAATGC 1 cut(s) 226
BsnI GGCC 2 cut(s) 234, 312
Bso31I GGTCTC 1 cut(s) 20
Bsp1286I GDGCHC 2 cut(s) 96, 682
Bsp143I GATC 3 cut(s) 122, 325, 438
BspACI CCGC 1 cut(s) 773
BspANI GGCC 2 cut(s) 234, 312
BspCNI CTCAG 2 cut(s) 19, 849
BspLI GGNNCC 1 cut(s) 440
BspMAI CTGCAG 2 cut(s) 558, 687
BspMI ACCTGC 1 cut(s) 35
BspPI GGATC 4 cut(s) 130, 333, 433, 446
BspQI GCTCTTC 1 cut(s) 831
BspTNI GGTCTC 1 cut(s) 20
BsrI ACTGG 1 cut(s) 279
BssECI CCNNGG 2 cut(s) 21, 235
BssMI GATC 3 cut(s) 122, 325, 438
Bst2UI CCWGG 3 cut(s) 22, 236, 675
Bst4CI ACNGT 2 cut(s) 211, 394
Bst6I CTCTTC 1 cut(s) 831
BstC8I GCNNGC 4 cut(s) 80, 96, 232, 264
BstDEI CTNAG 2 cut(s) 6, 857
BstF5I GGATG 5 cut(s) 27, 60, 190, 457, 583
BstKTI GATC 3 cut(s) 125, 328, 441
BstMAI GTCTC 1 cut(s) 20
BstMBI GATC 3 cut(s) 122, 325, 438
BstMWI GCNNNNNNNGC 2 cut(s) 240, 430
BstNI CCWGG 3 cut(s) 22, 236, 675
BstNSI RCATGY 1 cut(s) 301
BstSCI CCNGG 3 cut(s) 20, 234, 673
BstSFI CTRYAG 2 cut(s) 554, 683
BstV1I GCAGC 3 cut(s) 253, 346, 540
BstX2I RGATCY 2 cut(s) 325, 438
BstXI CCANNNNNNTGG 1 cut(s) 385
BstYI RGATCY 2 cut(s) 325, 438
BsuI GTATCC 1 cut(s) 382
BsuRI GGCC 2 cut(s) 234, 312
BtgZI GCGATG 1 cut(s) 19
BtrI CACGTC 1 cut(s) 91
BtsCI GGATG 5 cut(s) 27, 60, 190, 457, 583
BtsI GCAGTG 3 cut(s) 195, 288, 563
BtsIMutI CAGTG 4 cut(s) 195, 272, 288, 563
BveI ACCTGC 1 cut(s) 35
Cac8I GCNNGC 4 cut(s) 80, 96, 232, 264
Cfr13I GGNCC 1 cut(s) 310
CseI GACGC 2 cut(s) 147, 439
Csp6I GTAC 1 cut(s) 798
CviAII CATG 3 cut(s) 134, 298, 331
CviQI GTAC 1 cut(s) 798
DdeI CTNAG 2 cut(s) 6, 857
DpnI GATC 3 cut(s) 124, 327, 440
DpnII GATC 3 cut(s) 122, 325, 438
DriI GACNNNNNGTC 1 cut(s) 381
EaeI YGGCCR 1 cut(s) 232
Eam1104I CTCTTC 1 cut(s) 831
Eam1105I GACNNNNNGTC 1 cut(s) 381
EarI CTCTTC 1 cut(s) 831
Ecl136II GAGCTC 1 cut(s) 680
Eco24I GRGCYC 1 cut(s) 682
Eco31I GGTCTC 1 cut(s) 20
Eco53kI GAGCTC 1 cut(s) 680
Eco57I CTGAAG 1 cut(s) 89
EcoICRI GAGCTC 1 cut(s) 680
EcoO109I RGGNCCY 1 cut(s) 310
EcoRII CCWGG 3 cut(s) 20, 234, 673
EcoT38I GRGCYC 1 cut(s) 682
FaeI CATG 3 cut(s) 137, 301, 334
FatI CATG 3 cut(s) 133, 297, 330
Fnu4HI GCNGC 3 cut(s) 267, 360, 554
FokI GGATG 5 cut(s) 14, 47, 197, 464, 590
FriOI GRGCYC 1 cut(s) 682
Fsp4HI GCNGC 3 cut(s) 267, 360, 554
GluI GCNGC 3 cut(s) 267, 360, 554
GsaI CCCAGC 1 cut(s) 510
GsuI CTGGAG 1 cut(s) 696
HaeIII GGCC 2 cut(s) 234, 312
HapII CCGG 1 cut(s) 39
HgaI GACGC 2 cut(s) 147, 439
Hin1II CATG 3 cut(s) 137, 301, 334
HincII GTYRAC 1 cut(s) 870
HindII GTYRAC 1 cut(s) 870
HinfI GANTC 3 cut(s) 143, 547, 776
HpaII CCGG 1 cut(s) 39
HphI GGTGA 1 cut(s) 10
Hpy166II GTNNAC 1 cut(s) 870
Hpy188I TCNGA 9 cut(s) 9, 72, 88, 148, 219, 325, 525, 706, 742
Hpy188III TCNNGA 5 cut(s) 126, 386, 442, 575, 596
Hpy8I GTNNAC 1 cut(s) 870
Hpy99I CGWCG 2 cut(s) 92, 163
HpyAV CCTTC 2 cut(s) 131, 301
HpyCH4III ACNGT 2 cut(s) 211, 394
HpyCH4IV ACGT 3 cut(s) 90, 542, 750
HpyCH4V TGCA 6 cut(s) 200, 514, 556, 642, 685, 814
HpyF10VI GCNNNNNNNGC 2 cut(s) 240, 430
HpyF3I CTNAG 2 cut(s) 6, 857
HpySE526I ACGT 3 cut(s) 90, 542, 750
Hsp92II CATG 3 cut(s) 137, 301, 334
Kzo9I GATC 3 cut(s) 122, 325, 438
LguI GCTCTTC 1 cut(s) 831
LmnI GCTCC 2 cut(s) 41, 677
Lsp1109I GCAGC 3 cut(s) 253, 346, 540
MaeII ACGT 3 cut(s) 90, 542, 750
MaeIII GTNAC 3 cut(s) 47, 337, 364
MalI GATC 3 cut(s) 124, 327, 440
MboI GATC 3 cut(s) 122, 325, 438
MboII GAAGA 3 cut(s) 595, 611, 848
MflI RGATCY 2 cut(s) 325, 438
MhlI GDGCHC 2 cut(s) 96, 682
MlsI TGGCCA 1 cut(s) 234
MluCI AATT 5 cut(s) 317, 445, 489, 663, 768
MluNI TGGCCA 1 cut(s) 234
MlyI GAGTC 1 cut(s) 556
MmeI TCCRAC 1 cut(s) 111
MnlI CCTC 7 cut(s) 41, 67, 74, 429, 647, 852, 852
Mox20I TGGCCA 1 cut(s) 234
MscI TGGCCA 1 cut(s) 234
MseI TTAA 4 cut(s) 315, 411, 492, 762
MslI CAYNNNNRTG 1 cut(s) 335
Msp20I TGGCCA 1 cut(s) 234
MspA1I CMGCKG 2 cut(s) 266, 553
MspI CCGG 1 cut(s) 39
MspR9I CCNGG 3 cut(s) 22, 236, 675
Mva1269I GAATGC 1 cut(s) 226
MvaI CCWGG 3 cut(s) 22, 236, 675
MwoI GCNNNNNNNGC 2 cut(s) 240, 430
NdeII GATC 3 cut(s) 122, 325, 438
NlaIII CATG 3 cut(s) 137, 301, 334
NlaIV GGNNCC 1 cut(s) 440
NmuCI GTSAC 1 cut(s) 337
NspI RCATGY 1 cut(s) 301
PciI ACATGT 1 cut(s) 297
PciSI GCTCTTC 1 cut(s) 831
PcsI WCGNNNNNNNCGW 2 cut(s) 199, 721
PctI GAATGC 1 cut(s) 226
PfeI GAWTC 2 cut(s) 143, 776
PflMI CCANNNNNTGG 1 cut(s) 448
PkrI GCNGC 3 cut(s) 268, 361, 555
PleI GAGTC 1 cut(s) 555
PpsI GAGTC 1 cut(s) 555
PscI ACATGT 1 cut(s) 297
PsiI TTATAA 1 cut(s) 257
Psp124BI GAGCTC 1 cut(s) 682
Psp6I CCWGG 3 cut(s) 20, 234, 673
PspFI CCCAGC 1 cut(s) 506
PspGI CCWGG 3 cut(s) 20, 234, 673
PspN4I GGNNCC 1 cut(s) 440
PspPI GGNCC 1 cut(s) 310
PstI CTGCAG 2 cut(s) 558, 687
PsuI RGATCY 2 cut(s) 325, 438
PvuII CAGCTG 2 cut(s) 266, 553
RsaI GTAC 1 cut(s) 799
RsaNI GTAC 1 cut(s) 798
RseI CAYNNNNRTG 1 cut(s) 335
SacI GAGCTC 1 cut(s) 682
SapI GCTCTTC 1 cut(s) 831
SaqAI TTAA 4 cut(s) 315, 411, 492, 762
SatI GCNGC 3 cut(s) 267, 360, 554
Sau3AI GATC 3 cut(s) 122, 325, 438
Sau96I GGNCC 1 cut(s) 310
ScaI AGTACT 1 cut(s) 799
SchI GAGTC 1 cut(s) 556
ScrFI CCNGG 3 cut(s) 22, 236, 675
SduI GDGCHC 2 cut(s) 96, 682
SfcI CTRYAG 2 cut(s) 554, 683
SmiMI CAYNNNNRTG 1 cut(s) 335
Sse9I AATT 5 cut(s) 317, 445, 489, 663, 768
SsiI CCGC 1 cut(s) 773
SspI AATATT 1 cut(s) 518
SstI GAGCTC 1 cut(s) 682
StyD4I CCNGG 3 cut(s) 20, 234, 673
TaaI ACNGT 2 cut(s) 211, 394
TaiI ACGT 3 cut(s) 93, 545, 753
TasI AATT 5 cut(s) 317, 445, 489, 663, 768
TatI WGTACW 1 cut(s) 797
TfiI GAWTC 2 cut(s) 143, 776
Tru1I TTAA 4 cut(s) 315, 411, 492, 762
Tru9I TTAA 4 cut(s) 315, 411, 492, 762
TscAI CASTG 4 cut(s) 202, 279, 288, 563
TseFI GTSAC 1 cut(s) 337
TseI GCWGC 3 cut(s) 266, 359, 553
Tsp45I GTSAC 1 cut(s) 337
TspDTI ATGAA 2 cut(s) 150, 489
TspRI CASTG 4 cut(s) 202, 279, 288, 563
Van91I CCANNNNNTGG 1 cut(s) 448
XapI RAATTY 2 cut(s) 317, 445
XceI RCATGY 1 cut(s) 301
ZrmI AGTACT 1 cut(s) 799
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.