MD13G1078200.v1.1

Protein of unknown function (DUF1664)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0003884
Physical Location & Seq
Forward (+)
5503401 .. 5507770
4370 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1078200.v1.1.491

Sequence Viewer

Length: 912 bp
ATGGCTATGCAGAGCGGAATAGGGGTTTCCAAGATCCTGATCTTAGCTGGGGCAGGGTATACTGGCACCATCCTTCTTAAGAACGGTAAATTATCCGAGCTGCTCGGTGAACTTCAGTCATTGTTGAATGGATCCGGGGAACACGCAGACGGTGACTATGACTCCCTCACAGCACAGATTAGCCGAATGACAGCTGAGATCAGGCAAATTGGCTCGTCGCGTGGCGGGGGAACGGTTTTTGTGAATGGGAACGGTAGCCAAATTGGTAATATGACATCACTAATAATGCCAGCAGCTACCTTGGGAGCAGTGGGTTATGGATACATGTGGTGGAAGGGTCTAAAATTCTCAGATATGATGTATGTGACAAAGCGTGGTATGGCTACTGCTGTTGAGAACTTGACTAAAAATCTGGATAACGTGAAGGAGGCTGTTGCTAAAGCAAAGAAGCACCTGACGCAGAGGATTCAGCATGTGGATGACAAAATGGCGGAGCAAAATGAGCTCTCAAGGTCAATTAAGGAAGATGTTGCTGGAGTGCAACATTCTCTTACTGATATTGACTATGACTTGAGTAGATTGCAGAGCATGGTTTCTGGTTTGGATCATAAGATGGGTTCTCTTGAACAAAAGCAGGATCTTGCAAATCTTGGTGTTATCTACCTGGTCAATTTTGTCGATGGAAAAAAAGTTGAAATGCCTAAAACTAAGCAACTTGAAAACTCTGGGAAGTCACGTGGTCGTTTACTCTCATATTCAGATACTTCAGGCCTGATGGGTCTTAAAGAAATTGCGGACTCTCTGTCTGAAACATTAAATCCATCAACTGATGCTATTGTGAAAGATGATACTGAAATGACAGGAGAGTATCAGAAGAATAACCTAATAAGGTCAGTGTCAACCAGGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1664

Protein Analysis

304

Amino Acids

32.84

Weight (kDa)

6.54

Isoelectric Point (pI)

32.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1664 PF07889 89 - 212 1e-48 Protein of unknown function (DUF1664)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 65
AccBSI CCGCTC 1 cut(s) 15
AccI GTMKAC 1 cut(s) 59
AccII CGCG 1 cut(s) 220
AciI CCGC 4 cut(s) 15, 225, 491, 794
AclWI GGATC 5 cut(s) 28, 126, 139, 612, 645
AcsI RAATTY 1 cut(s) 344
AcuI CTGAAG 2 cut(s) 98, 750
AcvI CACGTG 1 cut(s) 737
AflII CTTAAG 1 cut(s) 77
AflIII ACRYGT 1 cut(s) 324
AgsI TTSAA 4 cut(s) 127, 626, 695, 719
AhdI GACNNNNNGTC 1 cut(s) 802
AjnI CCWGG 2 cut(s) 663, 902
AluBI AGCT 5 cut(s) 47, 100, 194, 296, 505
AluI AGCT 5 cut(s) 47, 100, 194, 296, 505
Alw21I GWGCWC 1 cut(s) 507
AlwI GGATC 5 cut(s) 28, 126, 139, 612, 645
AoxI GGCC 1 cut(s) 769
ApeKI GCWGC 2 cut(s) 100, 293
ApoI RAATTY 1 cut(s) 344
AsuC2I CCSGG 1 cut(s) 136
AsuHPI GGTGA 2 cut(s) 119, 164
BamHI GGATCC 1 cut(s) 131
BanI GGYRCC 1 cut(s) 65
BanII GRGCYC 1 cut(s) 507
BbrPI CACGTG 1 cut(s) 737
Bbv12I GWGCWC 1 cut(s) 507
BbvI GCAGC 2 cut(s) 87, 305
BccI CCATC 5 cut(s) 77, 607, 674, 769, 829
BciT130I CCWGG 2 cut(s) 665, 904
BciVI GTATCC 1 cut(s) 314
BcnI CCSGG 1 cut(s) 136
BfrI CTTAAG 1 cut(s) 77
BfuI GTATCC 1 cut(s) 314
BisI GCNGC 2 cut(s) 101, 294
BlsI GCNGC 2 cut(s) 102, 295
Bme1390I CCNGG 3 cut(s) 136, 665, 904
BmeRI GACNNNNNGTC 1 cut(s) 802
BmiI GGNNCC 2 cut(s) 67, 133
BmrFI CCNGG 3 cut(s) 136, 665, 904
BmsI GCATC 1 cut(s) 820
BpmI CTGGAG 1 cut(s) 555
BpuEI CTTGAG 2 cut(s) 493, 592
BpuMI CCSGG 1 cut(s) 136
BsaAI YACGTR 1 cut(s) 737
BsaBI GATNNNNATC 1 cut(s) 38
BsaJI CCNNGG 2 cut(s) 135, 300
BsaXI ACNNNNNCTCC 2 cut(s) 146, 176
Bse1I ACTGG 1 cut(s) 67
Bse8I GATNNNNATC 1 cut(s) 38
BseBI CCWGG 2 cut(s) 665, 904
BseDI CCNNGG 2 cut(s) 135, 300
BseGI GGATG 2 cut(s) 69, 484
BseJI GATNNNNATC 1 cut(s) 38
BseMII CTCAG 2 cut(s) 186, 363
BseNI ACTGG 1 cut(s) 67
BseXI GCAGC 2 cut(s) 87, 305
BseYI CCCAGC 1 cut(s) 47
Bsh1236I CGCG 1 cut(s) 220
BshFI GGCC 1 cut(s) 771
BshNI GGYRCC 1 cut(s) 65
BsiHKAI GWGCWC 1 cut(s) 507
BsiSI CCGG 1 cut(s) 135
BsnI GGCC 1 cut(s) 771
Bsp1286I GDGCHC 1 cut(s) 507
Bsp143I GATC 6 cut(s) 33, 39, 131, 198, 604, 637
BspACI CCGC 4 cut(s) 15, 225, 491, 794
BspANI GGCC 1 cut(s) 771
BspCNI CTCAG 2 cut(s) 187, 362
BspFNI CGCG 1 cut(s) 220
BspLI GGNNCC 2 cut(s) 67, 133
BspPI GGATC 5 cut(s) 28, 126, 139, 612, 645
BspT107I GGYRCC 1 cut(s) 65
BspTI CTTAAG 1 cut(s) 77
BsrBI CCGCTC 1 cut(s) 15
BsrI ACTGG 1 cut(s) 67
BssECI CCNNGG 2 cut(s) 135, 300
BssMI GATC 6 cut(s) 33, 39, 131, 198, 604, 637
BssNAI GTATAC 1 cut(s) 60
BssT1I CCWWGG 1 cut(s) 300
Bst1107I GTATAC 1 cut(s) 60
Bst2UI CCWGG 2 cut(s) 665, 904
Bst4CI ACNGT 4 cut(s) 86, 152, 235, 254
BstAFI CTTAAG 1 cut(s) 77
BstBAI YACGTR 1 cut(s) 737
BstC8I GCNNGC 1 cut(s) 291
BstDEI CTNAG 4 cut(s) 43, 195, 349, 708
BstF5I GGATG 2 cut(s) 69, 484
BstFNI CGCG 1 cut(s) 220
BstKTI GATC 6 cut(s) 36, 42, 134, 201, 607, 640
BstMBI GATC 6 cut(s) 33, 39, 131, 198, 604, 637
BstMWI GCNNNNNNNGC 2 cut(s) 457, 502
BstNI CCWGG 2 cut(s) 665, 904
BstNSI RCATGY 2 cut(s) 328, 476
BstSCI CCNGG 3 cut(s) 134, 663, 902
BstUI CGCG 1 cut(s) 220
BstV1I GCAGC 2 cut(s) 87, 305
BstX2I RGATCY 3 cut(s) 33, 131, 637
BstYI RGATCY 3 cut(s) 33, 131, 637
BstZ17I GTATAC 1 cut(s) 60
BsuI GTATCC 1 cut(s) 314
BsuRI GGCC 1 cut(s) 771
BtsCI GGATG 2 cut(s) 69, 484
BtsI GCAGTG 1 cut(s) 315
BtsIMutI CAGTG 2 cut(s) 315, 900
Cac8I GCNNGC 1 cut(s) 291
CseI GACGC 1 cut(s) 466
CsiI ACCWGGT 2 cut(s) 663, 902
CviAII CATG 3 cut(s) 325, 473, 589
DdeI CTNAG 4 cut(s) 43, 195, 349, 708
DpnI GATC 6 cut(s) 35, 41, 133, 200, 606, 639
DpnII GATC 6 cut(s) 33, 39, 131, 198, 604, 637
DriI GACNNNNNGTC 1 cut(s) 802
Eam1105I GACNNNNNGTC 1 cut(s) 802
EciI GGCGGA 1 cut(s) 506
Ecl136II GAGCTC 1 cut(s) 505
Eco130I CCWWGG 1 cut(s) 300
Eco147I AGGCCT 1 cut(s) 771
Eco24I GRGCYC 1 cut(s) 507
Eco53kI GAGCTC 1 cut(s) 505
Eco57I CTGAAG 2 cut(s) 98, 750
Eco72I CACGTG 1 cut(s) 737
EcoICRI GAGCTC 1 cut(s) 505
EcoRII CCWGG 2 cut(s) 663, 902
EcoT14I CCWWGG 1 cut(s) 300
EcoT38I GRGCYC 1 cut(s) 507
ErhI CCWWGG 1 cut(s) 300
FaeI CATG 3 cut(s) 328, 476, 592
FatI CATG 3 cut(s) 324, 472, 588
FauI CCCGC 1 cut(s) 218
FblI GTMKAC 1 cut(s) 59
Fnu4HI GCNGC 2 cut(s) 101, 294
FokI GGATG 2 cut(s) 56, 491
FriOI GRGCYC 1 cut(s) 507
Fsp4HI GCNGC 2 cut(s) 101, 294
GluI GCNGC 2 cut(s) 101, 294
GsaI CCCAGC 1 cut(s) 51
GsuI CTGGAG 1 cut(s) 555
HaeIII GGCC 1 cut(s) 771
HapII CCGG 1 cut(s) 135
HgaI GACGC 1 cut(s) 466
Hin1II CATG 3 cut(s) 328, 476, 592
HincII GTYRAC 1 cut(s) 900
HindII GTYRAC 1 cut(s) 900
HinfI GANTC 3 cut(s) 161, 466, 797
HpaII CCGG 1 cut(s) 135
HphI GGTGA 2 cut(s) 119, 164
Hpy166II GTNNAC 4 cut(s) 60, 110, 746, 900
Hpy188I TCNGA 5 cut(s) 97, 352, 760, 808, 873
Hpy188III TCNNGA 3 cut(s) 37, 413, 623
Hpy8I GTNNAC 4 cut(s) 60, 110, 746, 900
Hpy99I CGWCG 1 cut(s) 220
HpyAV CCTTC 3 cut(s) 83, 328, 418
HpyCH4III ACNGT 4 cut(s) 86, 152, 235, 254
HpyCH4IV ACGT 2 cut(s) 420, 736
HpyCH4V TGCA 4 cut(s) 10, 541, 583, 644
HpyF10VI GCNNNNNNNGC 2 cut(s) 457, 502
HpyF3I CTNAG 4 cut(s) 43, 195, 349, 708
HpySE526I ACGT 2 cut(s) 420, 736
Hsp92II CATG 3 cut(s) 328, 476, 592
Kzo9I GATC 6 cut(s) 33, 39, 131, 198, 604, 637
LmnI GCTCC 2 cut(s) 305, 493
Lsp1109I GCAGC 2 cut(s) 87, 305
LweI GCATC 1 cut(s) 820
MabI ACCWGGT 2 cut(s) 663, 902
MaeII ACGT 2 cut(s) 420, 736
MaeIII GTNAC 3 cut(s) 152, 364, 732
MalI GATC 6 cut(s) 35, 41, 133, 200, 606, 639
MbiI CCGCTC 1 cut(s) 15
MboI GATC 6 cut(s) 33, 39, 131, 198, 604, 637
MboII GAAGA 2 cut(s) 536, 886
MflI RGATCY 3 cut(s) 33, 131, 637
MhlI GDGCHC 1 cut(s) 507
MluCI AATT 7 cut(s) 89, 207, 261, 344, 516, 670, 789
MlyI GAGTC 2 cut(s) 155, 791
MnlI CCTC 3 cut(s) 176, 421, 456
MseI TTAA 4 cut(s) 78, 519, 783, 815
MslI CAYNNNNRTG 1 cut(s) 477
MspA1I CMGCKG 1 cut(s) 194
MspCI CTTAAG 1 cut(s) 77
MspI CCGG 1 cut(s) 135
MspR9I CCNGG 3 cut(s) 136, 665, 904
MvaI CCWGG 2 cut(s) 665, 904
MvnI CGCG 1 cut(s) 220
MwoI GCNNNNNNNGC 2 cut(s) 457, 502
NciI CCSGG 1 cut(s) 136
NdeII GATC 6 cut(s) 33, 39, 131, 198, 604, 637
NlaIII CATG 3 cut(s) 328, 476, 592
NlaIV GGNNCC 2 cut(s) 67, 133
NmuCI GTSAC 3 cut(s) 152, 364, 732
NspI RCATGY 2 cut(s) 328, 476
PceI AGGCCT 1 cut(s) 771
PciI ACATGT 1 cut(s) 324
PfeI GAWTC 1 cut(s) 466
PkrI GCNGC 2 cut(s) 102, 295
PleI GAGTC 2 cut(s) 155, 791
PmaCI CACGTG 1 cut(s) 737
PmlI CACGTG 1 cut(s) 737
PpsI GAGTC 2 cut(s) 155, 791
Ppu21I YACGTR 1 cut(s) 737
PscI ACATGT 1 cut(s) 324
Psp124BI GAGCTC 1 cut(s) 507
Psp6I CCWGG 2 cut(s) 663, 902
PspCI CACGTG 1 cut(s) 737
PspFI CCCAGC 1 cut(s) 47
PspGI CCWGG 2 cut(s) 663, 902
PspN4I GGNNCC 2 cut(s) 67, 133
PsuI RGATCY 3 cut(s) 33, 131, 637
PvuII CAGCTG 1 cut(s) 194
RseI CAYNNNNRTG 1 cut(s) 477
SacI GAGCTC 1 cut(s) 507
SaqAI TTAA 4 cut(s) 78, 519, 783, 815
SatI GCNGC 2 cut(s) 101, 294
Sau3AI GATC 6 cut(s) 33, 39, 131, 198, 604, 637
SchI GAGTC 2 cut(s) 155, 791
ScrFI CCNGG 3 cut(s) 136, 665, 904
SduI GDGCHC 1 cut(s) 507
SexAI ACCWGGT 2 cut(s) 663, 902
SfaNI GCATC 1 cut(s) 820
SmiMI CAYNNNNRTG 1 cut(s) 477
SmlI CTYRAG 3 cut(s) 77, 508, 571
SmoI CTYRAG 3 cut(s) 77, 508, 571
Sse9I AATT 7 cut(s) 89, 207, 261, 344, 516, 670, 789
SseBI AGGCCT 1 cut(s) 771
SsiI CCGC 4 cut(s) 15, 225, 491, 794
SstI GAGCTC 1 cut(s) 507
StuI AGGCCT 1 cut(s) 771
StyD4I CCNGG 3 cut(s) 134, 663, 902
StyI CCWWGG 1 cut(s) 300
TaaI ACNGT 4 cut(s) 86, 152, 235, 254
TaiI ACGT 2 cut(s) 423, 739
TaqI TCGA 1 cut(s) 678
TasI AATT 7 cut(s) 89, 207, 261, 344, 516, 670, 789
TfiI GAWTC 1 cut(s) 466
Tru1I TTAA 4 cut(s) 78, 519, 783, 815
Tru9I TTAA 4 cut(s) 78, 519, 783, 815
TscAI CASTG 2 cut(s) 315, 900
TseFI GTSAC 3 cut(s) 152, 364, 732
TseI GCWGC 2 cut(s) 100, 293
Tsp45I GTSAC 3 cut(s) 152, 364, 732
TspRI CASTG 2 cut(s) 315, 900
Vha464I CTTAAG 1 cut(s) 77
XapI RAATTY 1 cut(s) 344
XceI RCATGY 2 cut(s) 328, 476
XmiI GTMKAC 1 cut(s) 59
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.