Rorug04G0326500

Protein of unknown function (DUF1664)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0003884
Physical Location & Seq
Forward (+)
50150710 .. 50151725
1016 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0326500.1

Sequence Viewer

Length: 768 bp
ATGGAAGAAGAACAGGACTCAACAACCGTGCAACCACCGAGGGAAGAAGAAGGAGAGGACGCATCCCCGATCCGACCCGAGTCCGGAGAAGAAAACGCAGTCCGACTCGAATCGGATACAGCAACGGTCCAAGCCGTATCGGCAGAAGAGAACACGCAACCCCAGATCGAAGAAGAAGAAGAGGAACCACCGGTGGTTGAAGAAGACGACGCAGCCATGACCCGACCCGAATCCGGTGAAGAGAACGCAGTCGGATCTGAATCCGAAGAAGATAACATGGCACCGATCGAAATCGCATCGGAAGAAGAGAACACCGCAACGGTCCAACCCCTATCAGAAGGAGAGAAGGCAAAAGCGAACCGACTCGAACCGATCCGACTCGAACTTCCGACGGGTCGGGTCAAGAAGATAATGAAGCTGGACAGAGACATCAACAAAATAAACTCAGAAGCCCTACACCTGGTCTCATGCTCCACCCAGCTCTTCCTCCAATTCCTCGCCGAGGCCTCCGGCGAGGCGGCGATGGAGAAGAAGCGCAAGATCGTGAAGCTCGAACACATACGGACGGCGGCGAAGAGGCACCGGGCCACCAGAGATTTCCTCGTGGATGAGCTCCCCGTGCCGTCTCAGCCGTCCGATCAGAAGTCCGCTGATCGGAGCCGCTCTCGCCCTGTTGCTGACAATCCAGCTCCGGCTGGCACTCGCCGGATTGAGGACTTCTTTAGTAAGCCAGCAACTAAATCCCCTATTGAGGTTGAGGAGTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1664

Protein Analysis

255

Amino Acids

28.27

Weight (kDa)

4.53

Isoelectric Point (pI)

82.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBFD_NFYB_HMF PF00808 128 - 190 4.3e-16 Histone-like transcription factor (CBF/NF-Y) and archaeal histone
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 280, 579
AccBSI CCGCTC 1 cut(s) 663
AccIII TCCGGA 1 cut(s) 83
AciI CCGC 5 cut(s) 315, 518, 569, 648, 661
AclWI GGATC 3 cut(s) 64, 262, 367
AfiI CCNNNNNNNGG 7 cut(s) 83, 233, 460, 502, 654, 712, 751
AgeI ACCGGT 1 cut(s) 190
AgsI TTSAA 1 cut(s) 200
AjnI CCWGG 1 cut(s) 459
AluBI AGCT 5 cut(s) 418, 481, 550, 613, 689
AluI AGCT 5 cut(s) 418, 481, 550, 613, 689
Alw21I GWGCWC 1 cut(s) 615
Alw26I GTCTC 3 cut(s) 420, 469, 630
AlwI GGATC 3 cut(s) 64, 262, 367
Ama87I CYCGRG 1 cut(s) 77
Aor13HI TCCGGA 1 cut(s) 83
AoxI GGCC 2 cut(s) 504, 585
ApeKI GCWGC 1 cut(s) 212
AsiGI ACCGGT 1 cut(s) 190
AspLEI GCGC 1 cut(s) 537
AspS9I GGNCC 3 cut(s) 127, 322, 585
AsuC2I CCSGG 1 cut(s) 584
AsuHPI GGTGA 1 cut(s) 248
AvaI CYCGRG 1 cut(s) 77
AvaII GGWCC 2 cut(s) 127, 322
BanI GGYRCC 2 cut(s) 280, 579
BanII GRGCYC 1 cut(s) 615
BauI CACGAG 1 cut(s) 602
BbsI GAAGAC 1 cut(s) 210
Bbv12I GWGCWC 1 cut(s) 615
BbvI GCAGC 1 cut(s) 224
BccI CCATC 1 cut(s) 517
BceAI ACGGC 4 cut(s) 119, 582, 607, 616
BciT130I CCWGG 1 cut(s) 461
BciVI GTATCC 1 cut(s) 109
BcnI CCSGG 1 cut(s) 584
BcoDI GTCTC 3 cut(s) 420, 469, 630
BfaI CTAG 1 cut(s) 766
BfuI GTATCC 1 cut(s) 109
BglI GCCNNNNNGGC 1 cut(s) 140
BisI GCNGC 4 cut(s) 213, 519, 570, 661
BlsI GCNGC 4 cut(s) 214, 520, 571, 662
Bme1390I CCNGG 2 cut(s) 461, 584
Bme18I GGWCC 2 cut(s) 127, 322
BmeT110I CYCGRG 1 cut(s) 77
BmgT120I GGNCC 3 cut(s) 127, 322, 585
BmiI GGNNCC 4 cut(s) 186, 282, 581, 659
BmrFI CCNGG 2 cut(s) 461, 584
BmsI GCATC 2 cut(s) 71, 305
BoxI GACNNNNGTC 1 cut(s) 79
BpiI GAAGAC 1 cut(s) 210
BplI GAGNNNNNCTC 4 cut(s) 585, 617, 649, 681
BpuMI CCSGG 1 cut(s) 584
BsaBI GATNNNNATC 2 cut(s) 259, 290
BsaI GGTCTC 1 cut(s) 469
BsaJI CCNNGG 2 cut(s) 38, 501
BsaWI WCCGGW 3 cut(s) 83, 190, 233
Bsc4I CCNNNNNNNGG 7 cut(s) 83, 233, 460, 502, 654, 712, 751
Bse118I RCCGGY 1 cut(s) 190
Bse8I GATNNNNATC 2 cut(s) 259, 290
BseAI TCCGGA 1 cut(s) 83
BseBI CCWGG 1 cut(s) 461
BseDI CCNNGG 2 cut(s) 38, 501
BseGI GGATG 2 cut(s) 62, 613
BseJI GATNNNNATC 2 cut(s) 259, 290
BseLI CCNNNNNNNGG 7 cut(s) 83, 233, 460, 502, 654, 712, 751
BseMII CTCAG 2 cut(s) 459, 641
BseXI GCAGC 1 cut(s) 224
BseYI CCCAGC 1 cut(s) 477
Bsh1285I CGRYCG 1 cut(s) 288
BshFI GGCC 2 cut(s) 506, 587
BshNI GGYRCC 2 cut(s) 280, 579
BshTI ACCGGT 1 cut(s) 190
BsiEI CGRYCG 1 cut(s) 288
BsiHKAI GWGCWC 1 cut(s) 615
BsiHKCI CYCGRG 1 cut(s) 77
BsiSI CCGG 7 cut(s) 84, 191, 234, 510, 583, 692, 706
BslI CCNNNNNNNGG 7 cut(s) 83, 233, 460, 502, 654, 712, 751
BsmAI GTCTC 3 cut(s) 420, 469, 630
BsmBI CGTCTC 1 cut(s) 630
BsnI GGCC 2 cut(s) 506, 587
Bso31I GGTCTC 1 cut(s) 469
BsoBI CYCGRG 1 cut(s) 77
Bsp1286I GDGCHC 1 cut(s) 615
Bsp13I TCCGGA 1 cut(s) 83
Bsp143I GATC 8 cut(s) 69, 165, 254, 285, 372, 540, 637, 652
BspACI CCGC 5 cut(s) 315, 518, 569, 648, 661
BspANI GGCC 2 cut(s) 506, 587
BspCNI CTCAG 2 cut(s) 458, 640
BspEI TCCGGA 1 cut(s) 83
BspLI GGNNCC 4 cut(s) 186, 282, 581, 659
BspPI GGATC 3 cut(s) 64, 262, 367
BspQI GCTCTTC 1 cut(s) 488
BspT107I GGYRCC 2 cut(s) 280, 579
BspTNI GGTCTC 1 cut(s) 469
BsrBI CCGCTC 1 cut(s) 663
BsrFI RCCGGY 1 cut(s) 190
BssAI RCCGGY 1 cut(s) 190
BssECI CCNNGG 2 cut(s) 38, 501
BssMI GATC 8 cut(s) 69, 165, 254, 285, 372, 540, 637, 652
BssSI CACGAG 1 cut(s) 602
Bst2BI CACGAG 1 cut(s) 602
Bst2UI CCWGG 1 cut(s) 461
Bst4CI ACNGT 3 cut(s) 28, 127, 322
Bst6I CTCTTC 6 cut(s) 141, 174, 234, 300, 488, 569
BstC8I GCNNGC 2 cut(s) 697, 732
BstDEI CTNAG 2 cut(s) 445, 627
BstENI CCTNNNNNAGG 1 cut(s) 500
BstF5I GGATG 2 cut(s) 62, 613
BstHHI GCGC 1 cut(s) 537
BstKTI GATC 8 cut(s) 72, 168, 257, 288, 375, 543, 640, 655
BstMAI GTCTC 3 cut(s) 420, 469, 630
BstMBI GATC 8 cut(s) 69, 165, 254, 285, 372, 540, 637, 652
BstMCI CGRYCG 1 cut(s) 288
BstMWI GCNNNNNNNGC 4 cut(s) 140, 619, 628, 666
BstNI CCWGG 1 cut(s) 461
BstPAI GACNNNNGTC 1 cut(s) 79
BstSCI CCNGG 2 cut(s) 459, 582
BstV1I GCAGC 1 cut(s) 224
BstV2I GAAGAC 1 cut(s) 210
BstX2I RGATCY 1 cut(s) 254
BstYI RGATCY 1 cut(s) 254
BsuI GTATCC 1 cut(s) 109
BsuRI GGCC 2 cut(s) 506, 587
BtgZI GCGATG 1 cut(s) 536
BtsCI GGATG 2 cut(s) 62, 613
Cac8I GCNNGC 2 cut(s) 697, 732
CfoI GCGC 1 cut(s) 537
Cfr10I RCCGGY 1 cut(s) 190
Cfr13I GGNCC 3 cut(s) 127, 322, 585
CseI GACGC 2 cut(s) 68, 218
CsiI ACCWGGT 1 cut(s) 459
CspAI ACCGGT 1 cut(s) 190
CviAII CATG 3 cut(s) 217, 277, 468
DdeI CTNAG 2 cut(s) 445, 627
DpnI GATC 8 cut(s) 71, 167, 256, 287, 374, 542, 639, 654
DpnII GATC 8 cut(s) 69, 165, 254, 285, 372, 540, 637, 652
Eam1104I CTCTTC 6 cut(s) 141, 174, 234, 300, 488, 569
EarI CTCTTC 6 cut(s) 141, 174, 234, 300, 488, 569
Ecl136II GAGCTC 1 cut(s) 613
Eco147I AGGCCT 1 cut(s) 506
Eco24I GRGCYC 1 cut(s) 615
Eco31I GGTCTC 1 cut(s) 469
Eco47I GGWCC 2 cut(s) 127, 322
Eco53kI GAGCTC 1 cut(s) 613
Eco88I CYCGRG 1 cut(s) 77
EcoICRI GAGCTC 1 cut(s) 613
EcoNI CCTNNNNNAGG 1 cut(s) 500
EcoRII CCWGG 1 cut(s) 459
EcoT38I GRGCYC 1 cut(s) 615
Esp3I CGTCTC 1 cut(s) 630
FaeI CATG 3 cut(s) 220, 280, 471
FaiI YATR 4 cut(s) 218, 278, 469, 560
FatI CATG 3 cut(s) 216, 276, 467
Fnu4HI GCNGC 4 cut(s) 213, 519, 570, 661
FokI GGATG 2 cut(s) 49, 620
FriOI GRGCYC 1 cut(s) 615
Fsp4HI GCNGC 4 cut(s) 213, 519, 570, 661
FspBI CTAG 1 cut(s) 766
GlaI GCGC 1 cut(s) 536
GluI GCNGC 4 cut(s) 213, 519, 570, 661
GsaI CCCAGC 1 cut(s) 481
HaeIII GGCC 2 cut(s) 506, 587
HapII CCGG 7 cut(s) 84, 191, 234, 510, 583, 692, 706
HgaI GACGC 2 cut(s) 68, 218
HhaI GCGC 1 cut(s) 537
Hin1II CATG 3 cut(s) 220, 280, 471
Hin6I GCGC 1 cut(s) 535
HinP1I GCGC 1 cut(s) 535
HinfI GANTC 9 cut(s) 17, 80, 105, 110, 230, 260, 363, 378, 761
HpaII CCGG 7 cut(s) 84, 191, 234, 510, 583, 692, 706
HphI GGTGA 1 cut(s) 248
Hpy188III TCNNGA 3 cut(s) 84, 403, 544
Hpy99I CGWCG 2 cut(s) 212, 394
HpyAV CCTTC 3 cut(s) 44, 332, 340
HpyCH4III ACNGT 3 cut(s) 28, 127, 322
HpyCH4V TGCA 1 cut(s) 31
HpyF10VI GCNNNNNNNGC 4 cut(s) 140, 619, 628, 666
HpyF3I CTNAG 2 cut(s) 445, 627
Hsp92II CATG 3 cut(s) 220, 280, 471
HspAI GCGC 1 cut(s) 535
Kpn2I TCCGGA 1 cut(s) 83
Kzo9I GATC 8 cut(s) 69, 165, 254, 285, 372, 540, 637, 652
LguI GCTCTTC 1 cut(s) 488
LmnI GCTCC 4 cut(s) 476, 618, 657, 694
Lsp1109I GCAGC 1 cut(s) 224
LweI GCATC 2 cut(s) 71, 305
MabI ACCWGGT 1 cut(s) 459
MaeI CTAG 1 cut(s) 766
MalI GATC 8 cut(s) 71, 167, 256, 287, 374, 542, 639, 654
MbiI CCGCTC 1 cut(s) 663
MboI GATC 8 cut(s) 69, 165, 254, 285, 372, 540, 637, 652
MflI RGATCY 1 cut(s) 254
MhlI GDGCHC 1 cut(s) 615
MluCI AATT 1 cut(s) 491
MlyI GAGTC 5 cut(s) 11, 89, 99, 357, 372
MmeI TCCRAC 6 cut(s) 97, 127, 232, 349, 400, 413
MroI TCCGGA 1 cut(s) 83
MspA1I CMGCKG 1 cut(s) 650
MspI CCGG 7 cut(s) 84, 191, 234, 510, 583, 692, 706
MspR9I CCNGG 2 cut(s) 461, 584
MvaI CCWGG 1 cut(s) 461
MwoI GCNNNNNNNGC 4 cut(s) 140, 619, 628, 666
NciI CCSGG 1 cut(s) 584
NdeII GATC 8 cut(s) 69, 165, 254, 285, 372, 540, 637, 652
NlaIII CATG 3 cut(s) 220, 280, 471
NlaIV GGNNCC 4 cut(s) 186, 282, 581, 659
NmeAIII GCCGAG 1 cut(s) 526
PceI AGGCCT 1 cut(s) 506
PciSI GCTCTTC 1 cut(s) 488
PcsI WCGNNNNNNNCGW 1 cut(s) 549
PfeI GAWTC 3 cut(s) 110, 230, 260
PinAI ACCGGT 1 cut(s) 190
PkrI GCNGC 4 cut(s) 214, 520, 571, 662
Ple19I CGATCG 1 cut(s) 288
PleI GAGTC 5 cut(s) 11, 88, 99, 357, 372
PpsI GAGTC 5 cut(s) 11, 88, 99, 357, 372
PshAI GACNNNNGTC 1 cut(s) 79
Psp124BI GAGCTC 1 cut(s) 615
Psp6I CCWGG 1 cut(s) 459
PspFI CCCAGC 1 cut(s) 477
PspGI CCWGG 1 cut(s) 459
PspN4I GGNNCC 4 cut(s) 186, 282, 581, 659
PspPI GGNCC 3 cut(s) 127, 322, 585
PsuI RGATCY 1 cut(s) 254
PvuI CGATCG 1 cut(s) 288
SacI GAGCTC 1 cut(s) 615
SapI GCTCTTC 1 cut(s) 488
SatI GCNGC 4 cut(s) 213, 519, 570, 661
Sau3AI GATC 8 cut(s) 69, 165, 254, 285, 372, 540, 637, 652
Sau96I GGNCC 3 cut(s) 127, 322, 585
SchI GAGTC 5 cut(s) 11, 89, 99, 357, 372
ScrFI CCNGG 2 cut(s) 461, 584
SduI GDGCHC 1 cut(s) 615
SetI ASST 7 cut(s) 420, 462, 483, 552, 615, 691, 756
SexAI ACCWGGT 1 cut(s) 459
SfaNI GCATC 2 cut(s) 71, 305
SgrAI CRCCGGYG 1 cut(s) 190
SinI GGWCC 2 cut(s) 127, 322
Sse9I AATT 1 cut(s) 491
SseBI AGGCCT 1 cut(s) 506
SsiI CCGC 5 cut(s) 315, 518, 569, 648, 661
SspMI CTAG 1 cut(s) 766
SstI GAGCTC 1 cut(s) 615
StuI AGGCCT 1 cut(s) 506
StyD4I CCNGG 2 cut(s) 459, 582
TaaI ACNGT 3 cut(s) 28, 127, 322
TaqI TCGA 6 cut(s) 108, 168, 288, 366, 381, 552
TasI AATT 1 cut(s) 491
TauI GCSGC 3 cut(s) 521, 572, 663
TfiI GAWTC 3 cut(s) 110, 230, 260
TseI GCWGC 1 cut(s) 212
TspDTI ATGAA 1 cut(s) 428
TspGWI ACGGA 1 cut(s) 577
VpaK11BI GGWCC 2 cut(s) 127, 322
XagI CCTNNNNNAGG 1 cut(s) 500
XspI CTAG 1 cut(s) 766
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.