AT1G28760

NEMP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004377
Physical Location & Seq
Forward (+)
10103081 .. 10104883
1803 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G28760.1

Sequence Viewer

Length: 1431 bp
ATGACTACTTCTTCTTCTTTTCTTCGTCTTCCTCATCGAATCTGGTTTCTCCTTCCTCTTATTGTCTTTCTCTTGTTATCCTACGCGGCGGCGCTCAAAAGTGTTGGTCCCGGCGAGCCAGCTCTGAATATTAGCCTTAGGTCGTGCGAGCGAATCCAAGTTTCAGGCTATTCGAGAACCAAATTAGGGAAATTCGCTAACTCTCTCAGAGTAAACTTAGTACCAATCCCAGAAAAGCTACACCTCAAAATCCAAGTTTGTGTTCACCGAAATGCTACACTTGGAATGTGCCACTGTGAGAAATCTAATTGGAAAAATCTCCAGAGCAGCTCTGTTATATCACCATATGATAAACAATACATTGATGTGAGATTCACTGGTAGTGAATATCAGTCGTCTCGTTCAATGATTACGGTCAATGTTACAGAAGAGTTTCATCAATGGCGGATTTTTTGTCTTGTAGCGGGATTGATTGTGATTTTGTTAGCACCTGTTGTTAGCAGTTGGCTACCGTTTTACTATACGAGTTCTATGGCGGTTGGTGTTTTTCTCGTTGTTTTGATTATTATCTTCCAGGTTATGAGGTTATTACCTACTGGGAGGAAGAATGTTATGTATCTTGCGTTTTATGGATCTGTTGTTGGTGCTGGATCTTTCATTCTACATCAATTCTCAATGATGGTGAATATGATTCTTGTTAATTTTGGGTTGAGTGAGGATATGTATAATCCGGTAGCGATACTTGTGCTTGTTGGGGTGGTTATTACTGGAGCTGCTTTTGGATTTTGGACTGTGAGGAAGTTTGTTGTTTCGAAAGATGGAGGAGGTGTGGATGCTAGTGTTGCACAGTTTGTGAAATGGGCTATGCGTTCTGTTGCTGCTACGTTTATTTTGCAGAGTAGTCTTGATACTCCTATGGCCATGGGAGCTTTTGTTTTAGCTAGCTTGTTTGGTTTTCTTGTTAGTAAAACGAAAAGCTTTGTAGGGAAAAGTCATTGGTTGGTTTCTGCTGGTGAGAGACGTCCGATAATGCATGGACGAGCTGAGTTCCTTAGTCGCCCTGGAGGTGGTGGACTGTGGAAGAGTGCTAAGAGTGTACCTTCGTATTCCGGCTCTCCTTCTTATGATGTGATTGTGAGCTCACCGTCTTCAGTTAACCGGAGGATACCGATCGGAAATCAGGATTACTATTCTACATTCCATACGACGCCAAACCGGAAGAGGATGTCGAAGAGAGAGTATGACGAGTTGACACAAGAAACGACACGAGAAGCAATGGCAGGACTTGCAGCTTCTCCAGGATTTAGTGATTGGTTAGTTGAGCATGCTGACAGAATCAAGTTGCTTCCCACTGATAGTAGCTATGATGACGAATTGGGTAGTGAATCAGATTCAACCGGTGAACAATCTTGGACACGTTTCTTCTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NEMP

Protein Analysis

476

Amino Acids

52.92

Weight (kDa)

9.47

Isoelectric Point (pI)

50.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NEMP PF10225 138 - 353 1.9e-49 NEMP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1024
AccII CGCG 1 cut(s) 86
AciI CCGC 5 cut(s) 86, 89, 445, 464, 536
AclWI GGATC 2 cut(s) 640, 658
AcoI YGGCCR 1 cut(s) 918
AcsI RAATTY 1 cut(s) 191
AcuI CTGAAG 1 cut(s) 1134
AcyI GRCGYC 2 cut(s) 1021, 1208
AfaI GTAC 2 cut(s) 222, 1098
AfiI CCNNNNNNNGG 2 cut(s) 186, 1067
AflIII ACRYGT 1 cut(s) 1415
AgeI ACCGGT 1 cut(s) 1397
AgsI TTSAA 2 cut(s) 405, 1395
AjnI CCWGG 3 cut(s) 573, 1060, 1297
AjuI GAANNNNNNNTTGG 2 cut(s) 246, 278
Alw21I GWGCWC 1 cut(s) 1142
Alw26I GTCTC 2 cut(s) 402, 1012
AlwI GGATC 2 cut(s) 640, 658
AoxI GGCC 1 cut(s) 918
ApeKI GCWGC 4 cut(s) 327, 773, 878, 1289
ApoI RAATTY 1 cut(s) 191
AsiGI ACCGGT 1 cut(s) 1397
Asp700I GAANNNNTTC 1 cut(s) 432
AspLEI GCGC 1 cut(s) 94
AspS9I GGNCC 1 cut(s) 107
AsuC2I CCSGG 1 cut(s) 111
AsuHPI GGTGA 6 cut(s) 257, 333, 694, 1025, 1134, 1412
AsuII TTCGAA 1 cut(s) 812
AsuNHI GCTAGC 1 cut(s) 941
AvaII GGWCC 1 cut(s) 107
AxyI CCTNAGG 1 cut(s) 137
BalI TGGCCA 1 cut(s) 920
BanII GRGCYC 1 cut(s) 1142
BauI CACGAG 1 cut(s) 1266
BbsI GAAGAC 2 cut(s) 20, 1140
Bbv12I GWGCWC 1 cut(s) 1142
BbvI GCAGC 4 cut(s) 339, 760, 865, 1301
BccI CCATC 2 cut(s) 673, 812
BcgI CGANNNNNNTGC 2 cut(s) 727, 761
BciT130I CCWGG 3 cut(s) 575, 1062, 1299
BciVI GTATCC 1 cut(s) 1158
BcnI CCSGG 1 cut(s) 111
BcoDI GTCTC 2 cut(s) 402, 1012
BfaI CTAG 2 cut(s) 837, 942
BfoI RGCGCY 1 cut(s) 95
BfuI GTATCC 1 cut(s) 1158
BisI GCNGC 6 cut(s) 87, 90, 328, 774, 879, 1290
BlsI GCNGC 6 cut(s) 88, 91, 329, 775, 880, 1291
Bme1390I CCNGG 4 cut(s) 111, 575, 1062, 1299
Bme18I GGWCC 1 cut(s) 107
BmgT120I GGNCC 1 cut(s) 107
BmiI GGNNCC 1 cut(s) 109
BmrFI CCNGG 4 cut(s) 111, 575, 1062, 1299
BmrI ACTGGG 1 cut(s) 606
BmsI GCATC 1 cut(s) 823
BmtI GCTAGC 1 cut(s) 945
BmuI ACTGGG 1 cut(s) 606
BpiI GAAGAC 2 cut(s) 20, 1140
BpmI CTGGAG 4 cut(s) 305, 789, 1083, 1281
Bpu14I TTCGAA 1 cut(s) 812
BpuMI CCSGG 1 cut(s) 111
BsaBI GATNNNNATC 2 cut(s) 566, 1169
BsaHI GRCGYC 2 cut(s) 1021, 1208
BsaJI CCNNGG 2 cut(s) 921, 1060
BsaWI WCCGGW 4 cut(s) 730, 1158, 1215, 1397
BsaXI ACNNNNNCTCC 4 cut(s) 813, 843, 918, 948
Bsc4I CCNNNNNNNGG 2 cut(s) 186, 1067
Bse118I RCCGGY 1 cut(s) 1397
Bse1I ACTGG 3 cut(s) 382, 601, 772
Bse21I CCTNAGG 1 cut(s) 137
Bse3DI GCAATG 1 cut(s) 1281
Bse8I GATNNNNATC 2 cut(s) 566, 1169
BseBI CCWGG 3 cut(s) 575, 1062, 1299
BseDI CCNNGG 2 cut(s) 921, 1060
BseGI GGATG 2 cut(s) 838, 1230
BseJI GATNNNNATC 2 cut(s) 566, 1169
BseLI CCNNNNNNNGG 2 cut(s) 186, 1067
BseMI GCAATG 1 cut(s) 1281
BseMII CTCAG 2 cut(s) 220, 1035
BseNI ACTGG 3 cut(s) 382, 601, 772
BseRI GAGGAG 1 cut(s) 837
BseXI GCAGC 4 cut(s) 339, 760, 865, 1301
Bsh1236I CGCG 1 cut(s) 86
Bsh1285I CGRYCG 1 cut(s) 1173
BshFI GGCC 1 cut(s) 920
BshTI ACCGGT 1 cut(s) 1397
BsiEI CGRYCG 1 cut(s) 1173
BsiHKAI GWGCWC 1 cut(s) 1142
BsiSI CCGG 6 cut(s) 111, 731, 1110, 1159, 1216, 1398
BslFI GGGAC 1 cut(s) 93
BslI CCNNNNNNNGG 2 cut(s) 186, 1067
BsmAI GTCTC 2 cut(s) 402, 1012
BsmBI CGTCTC 2 cut(s) 402, 1012
BsmFI GGGAC 1 cut(s) 93
BsnI GGCC 1 cut(s) 920
Bsp119I TTCGAA 1 cut(s) 812
Bsp1286I GDGCHC 1 cut(s) 1142
Bsp143I GATC 3 cut(s) 632, 650, 1170
Bsp19I CCATGG 1 cut(s) 921
BspACI CCGC 5 cut(s) 86, 89, 445, 464, 536
BspANI GGCC 1 cut(s) 920
BspCNI CTCAG 2 cut(s) 219, 1036
BspFNI CGCG 1 cut(s) 86
BspLI GGNNCC 1 cut(s) 109
BspOI GCTAGC 1 cut(s) 945
BspPI GGATC 2 cut(s) 640, 658
BspT104I TTCGAA 1 cut(s) 812
BsrDI GCAATG 1 cut(s) 1281
BsrFI RCCGGY 1 cut(s) 1397
BsrI ACTGG 3 cut(s) 382, 601, 772
BssAI RCCGGY 1 cut(s) 1397
BssECI CCNNGG 2 cut(s) 921, 1060
BssMI GATC 3 cut(s) 632, 650, 1170
BssNI GRCGYC 2 cut(s) 1021, 1208
BssSI CACGAG 1 cut(s) 1266
BssT1I CCWWGG 1 cut(s) 921
Bst2BI CACGAG 1 cut(s) 1266
Bst2UI CCWGG 3 cut(s) 575, 1062, 1299
Bst4CI ACNGT 7 cut(s) 296, 415, 513, 793, 849, 1077, 1146
Bst6I CTCTTC 4 cut(s) 423, 1076, 1214, 1226
BstACI GRCGYC 2 cut(s) 1021, 1208
BstAPI GCANNNNNTGC 1 cut(s) 1286
BstBI TTCGAA 1 cut(s) 812
BstC8I GCNNGC 5 cut(s) 116, 120, 149, 943, 1326
BstDEI CTNAG 6 cut(s) 137, 206, 217, 1044, 1052, 1089
BstDSI CCRYGG 1 cut(s) 921
BstF5I GGATG 2 cut(s) 838, 1230
BstFNI CGCG 1 cut(s) 86
BstH2I RGCGCY 1 cut(s) 95
BstHHI GCGC 1 cut(s) 94
BstKTI GATC 3 cut(s) 635, 653, 1173
BstMAI GTCTC 2 cut(s) 402, 1012
BstMBI GATC 3 cut(s) 632, 650, 1170
BstMCI CGRYCG 1 cut(s) 1173
BstMWI GCNNNNNNNGC 3 cut(s) 842, 926, 1286
BstNI CCWGG 3 cut(s) 575, 1062, 1299
BstNSI RCATGY 1 cut(s) 1328
BstSCI CCNGG 4 cut(s) 109, 573, 1060, 1297
BstUI CGCG 1 cut(s) 86
BstV1I GCAGC 4 cut(s) 339, 760, 865, 1301
BstV2I GAAGAC 2 cut(s) 20, 1140
BstX2I RGATCY 2 cut(s) 632, 650
BstYI RGATCY 2 cut(s) 632, 650
Bsu36I CCTNAGG 1 cut(s) 137
BsuI GTATCC 1 cut(s) 1158
BsuRI GGCC 1 cut(s) 920
BtgI CCRYGG 1 cut(s) 921
BtsCI GGATG 2 cut(s) 838, 1230
BtsIMutI CAGTG 3 cut(s) 292, 375, 1350
Cac8I GCNNGC 5 cut(s) 116, 120, 149, 943, 1326
CfoI GCGC 1 cut(s) 94
Cfr10I RCCGGY 1 cut(s) 1397
Cfr13I GGNCC 1 cut(s) 107
CseI GACGC 1 cut(s) 1216
Csp6I GTAC 2 cut(s) 221, 1097
CspAI ACCGGT 1 cut(s) 1397
CviAII CATG 3 cut(s) 922, 1034, 1325
CviQI GTAC 2 cut(s) 221, 1097
DdeI CTNAG 6 cut(s) 137, 206, 217, 1044, 1052, 1089
DpnI GATC 3 cut(s) 634, 652, 1172
DpnII GATC 3 cut(s) 632, 650, 1170
EaeI YGGCCR 1 cut(s) 918
Eam1104I CTCTTC 4 cut(s) 423, 1076, 1214, 1226
EarI CTCTTC 4 cut(s) 423, 1076, 1214, 1226
EciI GGCGGA 1 cut(s) 460
Ecl136II GAGCTC 1 cut(s) 1140
Eco130I CCWWGG 1 cut(s) 921
Eco24I GRGCYC 1 cut(s) 1142
Eco47I GGWCC 1 cut(s) 107
Eco53kI GAGCTC 1 cut(s) 1140
Eco57I CTGAAG 1 cut(s) 1134
Eco81I CCTNAGG 1 cut(s) 137
EcoICRI GAGCTC 1 cut(s) 1140
EcoRII CCWGG 3 cut(s) 573, 1060, 1297
EcoT14I CCWWGG 1 cut(s) 921
EcoT22I ATGCAT 1 cut(s) 1035
EcoT38I GRGCYC 1 cut(s) 1142
ErhI CCWWGG 1 cut(s) 921
Esp3I CGTCTC 2 cut(s) 402, 1012
FaeI CATG 3 cut(s) 925, 1037, 1328
FaqI GGGAC 1 cut(s) 93
FatI CATG 3 cut(s) 921, 1033, 1324
FauI CCCGC 1 cut(s) 457
FauNDI CATATG 1 cut(s) 346
Fnu4HI GCNGC 6 cut(s) 87, 90, 328, 774, 879, 1290
FokI GGATG 2 cut(s) 845, 1237
FriOI GRGCYC 1 cut(s) 1142
Fsp4HI GCNGC 6 cut(s) 87, 90, 328, 774, 879, 1290
FspBI CTAG 2 cut(s) 837, 942
GlaI GCGC 1 cut(s) 93
GluI GCNGC 6 cut(s) 87, 90, 328, 774, 879, 1290
GsuI CTGGAG 4 cut(s) 305, 789, 1083, 1281
HaeII RGCGCY 1 cut(s) 95
HaeIII GGCC 1 cut(s) 920
HapII CCGG 6 cut(s) 111, 731, 1110, 1159, 1216, 1398
HgaI GACGC 1 cut(s) 1216
HhaI GCGC 1 cut(s) 94
Hin1I GRCGYC 2 cut(s) 1021, 1208
Hin1II CATG 3 cut(s) 925, 1037, 1328
Hin6I GCGC 1 cut(s) 92
HinP1I GCGC 1 cut(s) 92
HincII GTYRAC 2 cut(s) 1156, 1251
HindII GTYRAC 2 cut(s) 1156, 1251
HindIII AAGCTT 1 cut(s) 976
HinfI GANTC 7 cut(s) 39, 153, 372, 691, 1335, 1385, 1391
HpaI GTTAAC 1 cut(s) 1156
HpaII CCGG 6 cut(s) 111, 731, 1110, 1159, 1216, 1398
HphI GGTGA 6 cut(s) 257, 333, 694, 1025, 1134, 1412
Hpy166II GTNNAC 7 cut(s) 214, 265, 1073, 1097, 1156, 1251, 1403
Hpy188I TCNGA 5 cut(s) 126, 209, 1026, 1175, 1390
Hpy188III TCNNGA 4 cut(s) 174, 322, 905, 1181
Hpy8I GTNNAC 7 cut(s) 214, 265, 1073, 1097, 1156, 1251, 1403
Hpy99I CGWCG 1 cut(s) 1210
HpyAV CCTTC 3 cut(s) 62, 1110, 1128
HpyCH4III ACNGT 7 cut(s) 296, 415, 513, 793, 849, 1077, 1146
HpyCH4IV ACGT 3 cut(s) 884, 1021, 1417
HpyCH4V TGCA 4 cut(s) 845, 895, 1033, 1289
HpyF10VI GCNNNNNNNGC 3 cut(s) 842, 926, 1286
HpyF3I CTNAG 6 cut(s) 137, 206, 217, 1044, 1052, 1089
HpySE526I ACGT 3 cut(s) 884, 1021, 1417
Hsp92I GRCGYC 2 cut(s) 1021, 1208
Hsp92II CATG 3 cut(s) 925, 1037, 1328
HspAI GCGC 1 cut(s) 92
KspAI GTTAAC 1 cut(s) 1156
Kzo9I GATC 3 cut(s) 632, 650, 1170
LmnI GCTCC 2 cut(s) 770, 926
Lsp1109I GCAGC 4 cut(s) 339, 760, 865, 1301
LweI GCATC 1 cut(s) 823
MaeI CTAG 2 cut(s) 837, 942
MaeII ACGT 3 cut(s) 884, 1021, 1417
MaeIII GTNAC 1 cut(s) 421
MalI GATC 3 cut(s) 634, 652, 1172
MboI GATC 3 cut(s) 632, 650, 1170
MflI RGATCY 2 cut(s) 632, 650
MhlI GDGCHC 1 cut(s) 1142
MlsI TGGCCA 1 cut(s) 920
MluCI AATT 6 cut(s) 182, 191, 307, 668, 700, 1373
MluNI TGGCCA 1 cut(s) 920
Mox20I TGGCCA 1 cut(s) 920
Mph1103I ATGCAT 1 cut(s) 1035
MroXI GAANNNNTTC 1 cut(s) 432
MscI TGGCCA 1 cut(s) 920
MseI TTAA 2 cut(s) 699, 1155
MslI CAYNNNNRTG 2 cut(s) 270, 365
Msp20I TGGCCA 1 cut(s) 920
MspI CCGG 6 cut(s) 111, 731, 1110, 1159, 1216, 1398
MspR9I CCNGG 4 cut(s) 111, 575, 1062, 1299
MvaI CCWGG 3 cut(s) 575, 1062, 1299
MvnI CGCG 1 cut(s) 86
MwoI GCNNNNNNNGC 3 cut(s) 842, 926, 1286
NciI CCSGG 1 cut(s) 111
NcoI CCATGG 1 cut(s) 921
NdeI CATATG 1 cut(s) 346
NdeII GATC 3 cut(s) 632, 650, 1170
NheI GCTAGC 1 cut(s) 941
NlaIII CATG 3 cut(s) 925, 1037, 1328
NlaIV GGNNCC 1 cut(s) 109
NsiI ATGCAT 1 cut(s) 1035
NspI RCATGY 1 cut(s) 1328
NspV TTCGAA 1 cut(s) 812
PaeI GCATGC 1 cut(s) 1328
PdmI GAANNNNTTC 1 cut(s) 432
PfeI GAWTC 7 cut(s) 39, 153, 372, 691, 1335, 1385, 1391
PfoI TCCNGGA 1 cut(s) 1297
PinAI ACCGGT 1 cut(s) 1397
PkrI GCNGC 6 cut(s) 88, 91, 329, 775, 880, 1291
Ple19I CGATCG 1 cut(s) 1173
Psp124BI GAGCTC 1 cut(s) 1142
Psp6I CCWGG 3 cut(s) 573, 1060, 1297
PspGI CCWGG 3 cut(s) 573, 1060, 1297
PspN4I GGNNCC 1 cut(s) 109
PspPI GGNCC 1 cut(s) 107
PsuI RGATCY 2 cut(s) 632, 650
PvuI CGATCG 1 cut(s) 1173
RsaI GTAC 2 cut(s) 222, 1098
RsaNI GTAC 2 cut(s) 221, 1097
RseI CAYNNNNRTG 2 cut(s) 270, 365
SacI GAGCTC 1 cut(s) 1142
SaqAI TTAA 2 cut(s) 699, 1155
SatI GCNGC 6 cut(s) 87, 90, 328, 774, 879, 1290
Sau3AI GATC 3 cut(s) 632, 650, 1170
Sau96I GGNCC 1 cut(s) 107
ScrFI CCNGG 4 cut(s) 111, 575, 1062, 1299
SduI GDGCHC 1 cut(s) 1142
SfaNI GCATC 1 cut(s) 823
SfuI TTCGAA 1 cut(s) 812
SinI GGWCC 1 cut(s) 107
SmiMI CAYNNNNRTG 2 cut(s) 270, 365
SphI GCATGC 1 cut(s) 1328
Sse9I AATT 6 cut(s) 182, 191, 307, 668, 700, 1373
SsiI CCGC 5 cut(s) 86, 89, 445, 464, 536
SspI AATATT 1 cut(s) 130
SspMI CTAG 2 cut(s) 837, 942
SstI GAGCTC 1 cut(s) 1142
StyD4I CCNGG 4 cut(s) 109, 573, 1060, 1297
StyI CCWWGG 1 cut(s) 921
TaaI ACNGT 7 cut(s) 296, 415, 513, 793, 849, 1077, 1146
TaiI ACGT 3 cut(s) 887, 1024, 1420
TaqI TCGA 4 cut(s) 37, 173, 812, 1229
TasI AATT 6 cut(s) 182, 191, 307, 668, 700, 1373
TauI GCSGC 2 cut(s) 89, 92
TfiI GAWTC 7 cut(s) 39, 153, 372, 691, 1335, 1385, 1391
Tru1I TTAA 2 cut(s) 699, 1155
Tru9I TTAA 2 cut(s) 699, 1155
TscAI CASTG 3 cut(s) 299, 382, 1357
TseI GCWGC 4 cut(s) 327, 773, 878, 1289
TspDTI ATGAA 2 cut(s) 425, 646
TspRI CASTG 3 cut(s) 299, 382, 1357
VpaK11BI GGWCC 1 cut(s) 107
XapI RAATTY 1 cut(s) 191
XceI RCATGY 1 cut(s) 1328
XmnI GAANNNNTTC 1 cut(s) 432
XspI CTAG 2 cut(s) 837, 942
ZraI GACGTC 1 cut(s) 1022
Zsp2I ATGCAT 1 cut(s) 1035
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.