Rh3BG363700

NEMP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0004377
Physical Location & Seq
Reverse (-)
44678821 .. 44685617
6797 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG363700.1

Sequence Viewer

Length: 1506 bp
ATGGCTACCCTTCCTCTCTTCGTTTTCTCTCTCTTTCTTTCGACCATCTCATTCTTATGTTCCACTACCCCTACCCCTACCCCTACCCCTACCGGGAAAGGTATTGATCTTGAAAATCCAGTACTAAATGTCAACCCAGCACCGCTTTCTCTTTCTGGGCACTTGCCTACCGCTGGTTCTAAAGATGTTTTATTTTGTGAACGGGTTCGAGTTTCTGGTATACCTAGATCAAAAATTGGGAGTTATGCAAATTCCTTTCGGATTACTCTGTCTCCATCTGTCGTAATCCCGGAGAGGTTGCATAGCAAAATTGAGGTCTGTTTTCACCGTATCGATTCGCTTGGACTCTGTCAGTGTAGAAATGATGAGTGGAAAACTCTCGAGAAAGGGCTATGGGAATCTGTCATGTCCCCTTATGAGGAGAAATATGTTGATGTCAAGTACATTGGCGATTTACTTGGGTCTTCCACCATCACTGTTGAAGAAGTTTCTCAGCAATGGCGCCTTTGGTTTCTAGCAGTGGGATTCATTTTACTGTTGCTGGCACCAAGCATCAGTAGTTGGGTTCATTTTTATTATAGCACTTCAATGGTTATAGGAGTTTTTATGGTCATTATATTCCTTCTCTTCCAGGGAATGAAATTGCTGCCATTTGGAAGAAAAAATTTCTTCTATCTTGTTACTGTATATGGGTCAGTGCTTGGAGCTGGATCTTATCTCTTTCATCACTTCTCTGTCATGGTAAATTCAATTCTTGTAAATTTTGGGATTAGTGAAGACATGCACAATCTGATCTCTAAATTTTTACTGGTGGCGATTATCCTTATGGGTGCTGCACTAGGTTACTGGATTGGGAGGAAGCTTTTCATCTCAAAGGATGGAACAGTAGATGTTGTTGTAGCCCAATTTGCTATGTGGGCAATCCGCTTTTTTGGAATCACGTTCATCTCTCAGAGCACTCTTGATACTCCTTTGGCTATTGGAGCGTTGGTTTCCTGCTTGGTTATCTGCAAACTTATCACTTTTGTGAAGTGGCATGGTAGAATGAATCAGTCAAATGGTGCGAGTGGGAGTCCTTTACAGCAGAAAGGAAGACACGTCATAGGAAGACACAGTCGTCCAGAGTTCCTTAGCAGGTCCAGCGCACTAGAGGCATGGAACAGTACAAGGGGTGCATCTGCTTGGTCTGATTCTCCTGTTAAAGGTGTTGTGTCGCCATCTTTTAGGACATGTGATCAACAGGAGTACTATTCAACCTTCCACAAGATGCGAAACCGAAAGAAGTTCACAAAGAAAGAGTGGGATGATTTTACAATGGAATCAACGCGGCATGCTCTAGCAAAATGGGCAGCATCTCCTGAATTCACCGATTGGGTTATCGAGCATGCCGACCGCATACAACTCCTTCCAAGCGATAGTTCAGATGAAACTGTAGGAAGTGAATCTGATTCTACTGATGAGAATGTCGTGGGGAGCACGGATAGGTGTGGATTTCTCAAATGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NEMP

Protein Analysis

501

Amino Acids

56.07

Weight (kDa)

8.47

Isoelectric Point (pI)

39.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NEMP PF10225 158 - 403 4.6e-39 NEMP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1116
Acc36I ACCTGC 1 cut(s) 1125
AccB1I GGYRCC 2 cut(s) 501, 544
AccI GTMKAC 1 cut(s) 220
AccII CGCG 1 cut(s) 1327
AciI CCGC 5 cut(s) 143, 171, 925, 1327, 1393
AclWI GGATC 1 cut(s) 718
AcsI RAATTY 6 cut(s) 250, 664, 745, 760, 800, 1361
AcyI GRCGYC 1 cut(s) 502
AfaI GTAC 4 cut(s) 123, 443, 1165, 1247
AfiI CCNNNNNNNGG 4 cut(s) 93, 173, 418, 1202
AflIII ACRYGT 2 cut(s) 1096, 1229
AgsI TTSAA 5 cut(s) 113, 482, 588, 750, 1254
AjiI CACGTC 1 cut(s) 1099
AjnI CCWGG 1 cut(s) 630
AjuI GAANNNNNNNTTGG 2 cut(s) 1402, 1434
AleI CACNNNNGTG 1 cut(s) 1025
AluBI AGCT 2 cut(s) 707, 862
AluI AGCT 2 cut(s) 707, 862
Alw21I GWGCWC 2 cut(s) 959, 1478
Alw26I GTCTC 1 cut(s) 276
AlwI GGATC 1 cut(s) 718
Ama87I CYCGRG 1 cut(s) 380
ApeKI GCWGC 3 cut(s) 646, 833, 1349
ApoI RAATTY 6 cut(s) 250, 664, 745, 760, 800, 1361
ArsI GACNNNNNNTTYG 2 cut(s) 300, 332
Asp700I GAANNNNTTC 2 cut(s) 204, 863
AspLEI GCGC 2 cut(s) 504, 1145
AspS9I GGNCC 1 cut(s) 1137
AsuC2I CCSGG 2 cut(s) 94, 290
AsuHPI GGTGA 2 cut(s) 317, 1357
AvaI CYCGRG 1 cut(s) 380
AvaII GGWCC 1 cut(s) 1137
BaeGI GKGCMC 1 cut(s) 162
BanI GGYRCC 2 cut(s) 501, 544
BbsI GAAGAC 4 cut(s) 456, 783, 1099, 1114
Bbv12I GWGCWC 2 cut(s) 959, 1478
BbvI GCAGC 3 cut(s) 633, 820, 1361
BccI CCATC 5 cut(s) 53, 283, 479, 872, 1225
BciT130I CCWGG 1 cut(s) 632
BclI TGATCA 1 cut(s) 1234
BcnI CCSGG 2 cut(s) 94, 290
BcoDI GTCTC 1 cut(s) 276
BfaI CTAG 5 cut(s) 225, 515, 839, 1148, 1337
BfmI CTRYAG 1 cut(s) 1431
BfoI RGCGCY 1 cut(s) 505
BfuAI ACCTGC 1 cut(s) 1125
BisI GCNGC 4 cut(s) 647, 834, 1328, 1350
BlsI GCNGC 4 cut(s) 648, 835, 1329, 1351
BmcAI AGTACT 2 cut(s) 123, 1247
Bme1390I CCNGG 3 cut(s) 94, 290, 632
Bme18I GGWCC 1 cut(s) 1137
BmeT110I CYCGRG 1 cut(s) 380
BmgBI CACGTC 1 cut(s) 1099
BmgT120I GGNCC 1 cut(s) 1137
BmiI GGNNCC 2 cut(s) 503, 546
BmrFI CCNGG 3 cut(s) 94, 290, 632
BmsI GCATC 4 cut(s) 561, 1184, 1257, 1361
BpiI GAAGAC 4 cut(s) 456, 783, 1099, 1114
Bpu10I CCTNAGC 1 cut(s) 1130
BpuMI CCSGG 2 cut(s) 94, 290
Bsa29I ATCGAT 1 cut(s) 333
BsaHI GRCGYC 1 cut(s) 502
BsaJI CCNNGG 1 cut(s) 631
BsaXI ACNNNNNCTCC 4 cut(s) 256, 286, 975, 1005
Bsc4I CCNNNNNNNGG 4 cut(s) 93, 173, 418, 1202
Bse1I ACTGG 3 cut(s) 119, 813, 851
Bse3DI GCAATG 1 cut(s) 503
BseBI CCWGG 1 cut(s) 632
BseCI ATCGAT 1 cut(s) 333
BseDI CCNNGG 1 cut(s) 631
BseGI GGATG 2 cut(s) 883, 1309
BseLI CCNNNNNNNGG 4 cut(s) 93, 173, 418, 1202
BseMI GCAATG 1 cut(s) 503
BseMII CTCAG 2 cut(s) 506, 965
BseNI ACTGG 3 cut(s) 119, 813, 851
BseRI GAGGAG 1 cut(s) 434
BseSI GKGCMC 1 cut(s) 162
BseXI GCAGC 3 cut(s) 633, 820, 1361
BseYI CCCAGC 1 cut(s) 136
BsgI GTGCAG 1 cut(s) 819
Bsh1236I CGCG 1 cut(s) 1327
Bsh1285I CGRYCG 1 cut(s) 1393
BshNI GGYRCC 2 cut(s) 501, 544
BshVI ATCGAT 1 cut(s) 333
BsiEI CGRYCG 1 cut(s) 1393
BsiHKAI GWGCWC 2 cut(s) 959, 1478
BsiHKCI CYCGRG 1 cut(s) 380
BsiSI CCGG 2 cut(s) 93, 290
BslFI GGGAC 1 cut(s) 394
BslI CCNNNNNNNGG 4 cut(s) 93, 173, 418, 1202
BsmAI GTCTC 1 cut(s) 276
BsmFI GGGAC 1 cut(s) 394
BsoBI CYCGRG 1 cut(s) 380
Bsp1286I GDGCHC 3 cut(s) 162, 959, 1478
Bsp143I GATC 5 cut(s) 106, 227, 710, 792, 1234
BspACI CCGC 5 cut(s) 143, 171, 925, 1327, 1393
BspCNI CTCAG 2 cut(s) 505, 964
BspDI ATCGAT 1 cut(s) 333
BspFNI CGCG 1 cut(s) 1327
BspLI GGNNCC 2 cut(s) 503, 546
BspMI ACCTGC 1 cut(s) 1125
BspPI GGATC 1 cut(s) 718
BspT107I GGYRCC 2 cut(s) 501, 544
BsrDI GCAATG 1 cut(s) 503
BsrI ACTGG 3 cut(s) 119, 813, 851
BssECI CCNNGG 1 cut(s) 631
BssMI GATC 5 cut(s) 106, 227, 710, 792, 1234
BssNAI GTATAC 1 cut(s) 221
BssNI GRCGYC 1 cut(s) 502
Bst1107I GTATAC 1 cut(s) 221
Bst2UI CCWGG 1 cut(s) 632
Bst4CI ACNGT 8 cut(s) 329, 478, 537, 685, 886, 1115, 1163, 1432
Bst6I CTCTTC 2 cut(s) 23, 632
BstACI GRCGYC 1 cut(s) 502
BstC8I GCNNGC 3 cut(s) 543, 1332, 1386
BstDEI CTNAG 3 cut(s) 492, 951, 1130
BstENI CCTNNNNNAGG 1 cut(s) 1200
BstF5I GGATG 2 cut(s) 883, 1309
BstFNI CGCG 1 cut(s) 1327
BstH2I RGCGCY 1 cut(s) 505
BstHHI GCGC 2 cut(s) 504, 1145
BstKTI GATC 5 cut(s) 109, 230, 713, 795, 1237
BstMAI GTCTC 1 cut(s) 276
BstMBI GATC 5 cut(s) 106, 227, 710, 792, 1234
BstMCI CGRYCG 1 cut(s) 1393
BstMWI GCNNNNNNNGC 6 cut(s) 908, 917, 983, 1140, 1151, 1346
BstNI CCWGG 1 cut(s) 632
BstNSI RCATGY 4 cut(s) 784, 1233, 1334, 1388
BstSCI CCNGG 3 cut(s) 92, 288, 630
BstSFI CTRYAG 1 cut(s) 1431
BstSLI GKGCMC 1 cut(s) 162
BstUI CGCG 1 cut(s) 1327
BstV1I GCAGC 3 cut(s) 633, 820, 1361
BstV2I GAAGAC 4 cut(s) 456, 783, 1099, 1114
BstX2I RGATCY 1 cut(s) 710
BstYI RGATCY 1 cut(s) 710
BstZ17I GTATAC 1 cut(s) 221
Bsu15I ATCGAT 1 cut(s) 333
BsuTUI ATCGAT 1 cut(s) 333
BtrI CACGTC 1 cut(s) 1099
BtsCI GGATG 2 cut(s) 883, 1309
BtsI GCAGTG 1 cut(s) 525
BtsIMutI CAGTG 4 cut(s) 359, 474, 525, 702
BveI ACCTGC 1 cut(s) 1125
Cac8I GCNNGC 3 cut(s) 543, 1332, 1386
CfoI GCGC 2 cut(s) 504, 1145
Cfr13I GGNCC 1 cut(s) 1137
ClaI ATCGAT 1 cut(s) 333
Csp6I GTAC 4 cut(s) 122, 442, 1164, 1246
CviAII CATG 8 cut(s) 406, 739, 781, 1037, 1155, 1230, 1331, 1385
CviJI RGCY 6 cut(s) 5, 391, 707, 862, 902, 977
CviKI_1 RGCY 6 cut(s) 5, 391, 707, 862, 902, 977
CviQI GTAC 4 cut(s) 122, 442, 1164, 1246
DdeI CTNAG 3 cut(s) 492, 951, 1130
DinI GGCGCC 1 cut(s) 503
DpnI GATC 5 cut(s) 108, 229, 712, 794, 1236
DpnII GATC 5 cut(s) 106, 227, 710, 792, 1234
DrdI GACNNNNNNGTC 1 cut(s) 1116
DseDI GACNNNNNNGTC 1 cut(s) 1116
Eam1104I CTCTTC 2 cut(s) 23, 632
EarI CTCTTC 2 cut(s) 23, 632
Eco47I GGWCC 1 cut(s) 1137
Eco88I CYCGRG 1 cut(s) 380
EcoNI CCTNNNNNAGG 1 cut(s) 1200
EcoRI GAATTC 1 cut(s) 1361
EcoRII CCWGG 1 cut(s) 630
EgeI GGCGCC 1 cut(s) 503
EheI GGCGCC 1 cut(s) 503
FaeI CATG 8 cut(s) 409, 742, 784, 1040, 1158, 1233, 1334, 1388
FaqI GGGAC 1 cut(s) 394
FatI CATG 8 cut(s) 405, 738, 780, 1036, 1154, 1229, 1330, 1384
FbaI TGATCA 1 cut(s) 1234
FblI GTMKAC 1 cut(s) 220
Fnu4HI GCNGC 4 cut(s) 647, 834, 1328, 1350
FokI GGATG 2 cut(s) 890, 1316
Fsp4HI GCNGC 4 cut(s) 647, 834, 1328, 1350
FspBI CTAG 5 cut(s) 225, 515, 839, 1148, 1337
GlaI GCGC 2 cut(s) 503, 1144
GluI GCNGC 4 cut(s) 647, 834, 1328, 1350
GsaI CCCAGC 1 cut(s) 140
HaeII RGCGCY 1 cut(s) 505
HapII CCGG 2 cut(s) 93, 290
HhaI GCGC 2 cut(s) 504, 1145
Hin1I GRCGYC 1 cut(s) 502
Hin1II CATG 8 cut(s) 409, 742, 784, 1040, 1158, 1233, 1334, 1388
Hin6I GCGC 2 cut(s) 502, 1143
HinP1I GCGC 2 cut(s) 502, 1143
HincII GTYRAC 1 cut(s) 133
HindII GTYRAC 1 cut(s) 133
HindIII AAGCTT 1 cut(s) 860
HpaII CCGG 2 cut(s) 93, 290
HphI GGTGA 2 cut(s) 317, 1357
Hpy166II GTNNAC 4 cut(s) 133, 200, 221, 1287
Hpy188I TCNGA 6 cut(s) 261, 792, 954, 1189, 1423, 1447
Hpy188III TCNNGA 6 cut(s) 110, 380, 382, 962, 1121, 1358
Hpy8I GTNNAC 4 cut(s) 133, 200, 221, 1287
HpyAV CCTTC 4 cut(s) 20, 632, 1267, 1415
HpyCH4III ACNGT 8 cut(s) 329, 478, 537, 685, 886, 1115, 1163, 1432
HpyCH4IV ACGT 2 cut(s) 941, 1098
HpyCH4V TGCA 6 cut(s) 248, 301, 784, 836, 1011, 1175
HpyF10VI GCNNNNNNNGC 6 cut(s) 908, 917, 983, 1140, 1151, 1346
HpyF3I CTNAG 3 cut(s) 492, 951, 1130
HpySE526I ACGT 2 cut(s) 941, 1098
Hsp92I GRCGYC 1 cut(s) 502
Hsp92II CATG 8 cut(s) 409, 742, 784, 1040, 1158, 1233, 1334, 1388
HspAI GCGC 2 cut(s) 502, 1143
KasI GGCGCC 1 cut(s) 501
Ksp22I TGATCA 1 cut(s) 1234
Kzo9I GATC 5 cut(s) 106, 227, 710, 792, 1234
LmnI GCTCC 3 cut(s) 704, 983, 1473
Lsp1109I GCAGC 3 cut(s) 633, 820, 1361
LweI GCATC 4 cut(s) 561, 1184, 1257, 1361
MaeI CTAG 5 cut(s) 225, 515, 839, 1148, 1337
MaeII ACGT 2 cut(s) 941, 1098
MaeIII GTNAC 2 cut(s) 679, 842
MalI GATC 5 cut(s) 108, 229, 712, 794, 1236
MboI GATC 5 cut(s) 106, 227, 710, 792, 1234
MboII GAAGA 9 cut(s) 10, 456, 494, 619, 661, 669, 788, 1104, 1119
MflI RGATCY 1 cut(s) 710
MhlI GDGCHC 3 cut(s) 162, 959, 1478
Mly113I GGCGCC 1 cut(s) 502
MlyI GAGTC 2 cut(s) 339, 1081
MnlI CCTC 6 cut(s) 24, 288, 307, 412, 849, 1144
MroXI GAANNNNTTC 2 cut(s) 204, 863
MseI TTAA 1 cut(s) 1200
MslI CAYNNNNRTG 3 cut(s) 55, 587, 1025
MspA1I CMGCKG 1 cut(s) 173
MspI CCGG 2 cut(s) 93, 290
MspR9I CCNGG 3 cut(s) 94, 290, 632
MvaI CCWGG 1 cut(s) 632
MvnI CGCG 1 cut(s) 1327
MwoI GCNNNNNNNGC 6 cut(s) 908, 917, 983, 1140, 1151, 1346
NarI GGCGCC 1 cut(s) 502
NciI CCSGG 2 cut(s) 94, 290
NdeII GATC 5 cut(s) 106, 227, 710, 792, 1234
NlaIII CATG 8 cut(s) 409, 742, 784, 1040, 1158, 1233, 1334, 1388
NlaIV GGNNCC 2 cut(s) 503, 546
NspI RCATGY 4 cut(s) 784, 1233, 1334, 1388
OliI CACNNNNGTG 1 cut(s) 1025
PaeI GCATGC 2 cut(s) 1334, 1388
PaeR7I CTCGAG 1 cut(s) 380
PciI ACATGT 1 cut(s) 1229
PcsI WCGNNNNNNNCGW 1 cut(s) 1386
PdmI GAANNNNTTC 2 cut(s) 204, 863
PfeI GAWTC 9 cut(s) 335, 398, 525, 936, 1048, 1190, 1319, 1442, 1448
PflFI GACNNNGTC 2 cut(s) 348, 1113
PfoI TCCNGGA 1 cut(s) 288
PkrI GCNGC 4 cut(s) 648, 835, 1329, 1351
PleI GAGTC 2 cut(s) 339, 1080
PluTI GGCGCC 1 cut(s) 505
PpsI GAGTC 2 cut(s) 339, 1080
PscI ACATGT 1 cut(s) 1229
Psp6I CCWGG 1 cut(s) 630
PspFI CCCAGC 1 cut(s) 136
PspGI CCWGG 1 cut(s) 630
PspN4I GGNNCC 2 cut(s) 503, 546
PspPI GGNCC 1 cut(s) 1137
PsuI RGATCY 1 cut(s) 710
PsyI GACNNNGTC 2 cut(s) 348, 1113
RsaI GTAC 4 cut(s) 123, 443, 1165, 1247
RsaNI GTAC 4 cut(s) 122, 442, 1164, 1246
RseI CAYNNNNRTG 3 cut(s) 55, 587, 1025
SaqAI TTAA 1 cut(s) 1200
SatI GCNGC 4 cut(s) 647, 834, 1328, 1350
Sau3AI GATC 5 cut(s) 106, 227, 710, 792, 1234
Sau96I GGNCC 1 cut(s) 1137
ScaI AGTACT 2 cut(s) 123, 1247
SchI GAGTC 2 cut(s) 339, 1081
ScrFI CCNGG 3 cut(s) 94, 290, 632
SduI GDGCHC 3 cut(s) 162, 959, 1478
SfaNI GCATC 4 cut(s) 561, 1184, 1257, 1361
SfcI CTRYAG 1 cut(s) 1431
SfoI GGCGCC 1 cut(s) 503
Sfr274I CTCGAG 1 cut(s) 380
SinI GGWCC 1 cut(s) 1137
SlaI CTCGAG 1 cut(s) 380
SmiMI CAYNNNNRTG 3 cut(s) 55, 587, 1025
SmlI CTYRAG 1 cut(s) 380
SmoI CTYRAG 1 cut(s) 380
SphI GCATGC 2 cut(s) 1334, 1388
SsiI CCGC 5 cut(s) 143, 171, 925, 1327, 1393
SspDI GGCGCC 1 cut(s) 501
SspMI CTAG 5 cut(s) 225, 515, 839, 1148, 1337
StyD4I CCNGG 3 cut(s) 92, 288, 630
TaaI ACNGT 8 cut(s) 329, 478, 537, 685, 886, 1115, 1163, 1432
TaiI ACGT 2 cut(s) 944, 1101
TaqI TCGA 5 cut(s) 41, 208, 333, 381, 1380
TatI WGTACW 4 cut(s) 121, 441, 1163, 1245
TauI GCSGC 1 cut(s) 1330
TfiI GAWTC 9 cut(s) 335, 398, 525, 936, 1048, 1190, 1319, 1442, 1448
Tru1I TTAA 1 cut(s) 1200
Tru9I TTAA 1 cut(s) 1200
TscAI CASTG 4 cut(s) 359, 481, 525, 702
TseI GCWGC 3 cut(s) 646, 833, 1349
TspDTI ATGAA 8 cut(s) 517, 557, 653, 713, 856, 934, 1061, 1440
TspGWI ACGGA 1 cut(s) 1493
TspRI CASTG 4 cut(s) 359, 481, 525, 702
Tth111I GACNNNGTC 2 cut(s) 348, 1113
VpaK11BI GGWCC 1 cut(s) 1137
XagI CCTNNNNNAGG 1 cut(s) 1200
XapI RAATTY 6 cut(s) 250, 664, 745, 760, 800, 1361
XceI RCATGY 4 cut(s) 784, 1233, 1334, 1388
XhoI CTCGAG 1 cut(s) 380
XmiI GTMKAC 1 cut(s) 220
XmnI GAANNNNTTC 2 cut(s) 204, 863
XspI CTAG 5 cut(s) 225, 515, 839, 1148, 1337
ZrmI AGTACT 2 cut(s) 123, 1247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.