Rh5AG246200

NEMP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0004377
Physical Location & Seq
Reverse (-)
30444030 .. 30445518
1489 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG246200.1

Sequence Viewer

Length: 654 bp
ATGGCTACCCTTCCTCTCTTCGTTTTCTCTCTCTTTCTTTCGACCATCTCATTCTTATGTTCCACTACCCCTACCGGGAAAGGTATTGATCTTGAAAATCAAGTACTAAATGTCAACCCAGCACCGCTTTCTCTTTTTGGGCACTCGCCTACCGCTGGTTCTAAAGATGTTTTATTTTGTGAACGGGTTCGAGTTTCTGGTGTACCTAGATCAAAAATTGGGAGTTATGCAAATTCCTTTCGGATTACTCTGTCTCCATCTGTCTTAATCCCAGAGAGGTTGCATAGCAAAATTGAGGTATGTTTTCACCGTAATGATTCGCTTGGACTCTGTCAGTGTAGAAATGATGAGTGGAAAACTCTCGAGAAAGGGCTATGGGAATCTGTCATGTCCCCTTATGAGGAGAAATATGTTGATGTCAAGTACATTGGCGATTTACTTGGGTCTTCCACCATCACTGTTGAAGAAGTTTCTCAGCAATGGCGCCTTTGGTTTCTAGCAGTGGGATTCATTTTACTATTGCTGGCACCAAGCATCAGTAGTTGGGTTCATTTTTATTATAGCACTTCAATGGTTATAGGAGTTTTTATGGTCATTATATTCCTTCTCTTCCAGATACTTTTTCTTTTCTTTTTCTGTTTTAGATGGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NEMP

Protein Analysis

217

Amino Acids

24.74

Weight (kDa)

6.89

Isoelectric Point (pI)

41.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NEMP PF10225 151 - 206 5.3e-14 NEMP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 483, 526
AciI CCGC 2 cut(s) 125, 153
AcsI RAATTY 1 cut(s) 232
AcyI GRCGYC 1 cut(s) 484
AfaI GTAC 3 cut(s) 105, 204, 425
AfiI CCNNNNNNNGG 3 cut(s) 75, 155, 400
AgsI TTSAA 3 cut(s) 95, 464, 570
Alw26I GTCTC 1 cut(s) 258
Ama87I CYCGRG 1 cut(s) 362
ApoI RAATTY 1 cut(s) 232
Asp700I GAANNNNTTC 1 cut(s) 186
AspLEI GCGC 1 cut(s) 486
AsuC2I CCSGG 1 cut(s) 76
AsuHPI GGTGA 1 cut(s) 299
AvaI CYCGRG 1 cut(s) 362
BaeGI GKGCMC 1 cut(s) 144
BanI GGYRCC 2 cut(s) 483, 526
BbsI GAAGAC 1 cut(s) 438
BccI CCATC 4 cut(s) 53, 265, 461, 639
BcnI CCSGG 1 cut(s) 76
BcoDI GTCTC 1 cut(s) 258
BfaI CTAG 2 cut(s) 207, 497
BfoI RGCGCY 1 cut(s) 487
BmcAI AGTACT 1 cut(s) 105
Bme1390I CCNGG 1 cut(s) 76
BmeT110I CYCGRG 1 cut(s) 362
BmiI GGNNCC 2 cut(s) 485, 528
BmrFI CCNGG 1 cut(s) 76
BmsI GCATC 1 cut(s) 543
BpiI GAAGAC 1 cut(s) 438
BpuMI CCSGG 1 cut(s) 76
BsaHI GRCGYC 1 cut(s) 484
BsaXI ACNNNNNCTCC 2 cut(s) 238, 268
Bsc4I CCNNNNNNNGG 3 cut(s) 75, 155, 400
Bse3DI GCAATG 1 cut(s) 485
BseLI CCNNNNNNNGG 3 cut(s) 75, 155, 400
BseMI GCAATG 1 cut(s) 485
BseMII CTCAG 1 cut(s) 488
BseRI GAGGAG 1 cut(s) 416
BseSI GKGCMC 1 cut(s) 144
BseYI CCCAGC 1 cut(s) 118
BshNI GGYRCC 2 cut(s) 483, 526
BsiHKCI CYCGRG 1 cut(s) 362
BsiSI CCGG 1 cut(s) 75
BslFI GGGAC 1 cut(s) 376
BslI CCNNNNNNNGG 3 cut(s) 75, 155, 400
BsmAI GTCTC 1 cut(s) 258
BsmFI GGGAC 1 cut(s) 376
BsoBI CYCGRG 1 cut(s) 362
Bsp1286I GDGCHC 1 cut(s) 144
Bsp143I GATC 2 cut(s) 88, 209
BspACI CCGC 2 cut(s) 125, 153
BspCNI CTCAG 1 cut(s) 487
BspLI GGNNCC 2 cut(s) 485, 528
BspT107I GGYRCC 2 cut(s) 483, 526
BsrDI GCAATG 1 cut(s) 485
BssMI GATC 2 cut(s) 88, 209
BssNI GRCGYC 1 cut(s) 484
Bst4CI ACNGT 2 cut(s) 311, 460
Bst6I CTCTTC 2 cut(s) 23, 614
BstACI GRCGYC 1 cut(s) 484
BstC8I GCNNGC 1 cut(s) 525
BstDEI CTNAG 1 cut(s) 474
BstH2I RGCGCY 1 cut(s) 487
BstHHI GCGC 1 cut(s) 486
BstKTI GATC 2 cut(s) 91, 212
BstMAI GTCTC 1 cut(s) 258
BstMBI GATC 2 cut(s) 88, 209
BstSCI CCNGG 1 cut(s) 74
BstSLI GKGCMC 1 cut(s) 144
BstV2I GAAGAC 1 cut(s) 438
BtsI GCAGTG 1 cut(s) 507
BtsIMutI CAGTG 3 cut(s) 341, 456, 507
Cac8I GCNNGC 1 cut(s) 525
CfoI GCGC 1 cut(s) 486
Csp6I GTAC 3 cut(s) 104, 203, 424
CviAII CATG 1 cut(s) 388
CviJI RGCY 2 cut(s) 5, 373
CviKI_1 RGCY 2 cut(s) 5, 373
CviQI GTAC 3 cut(s) 104, 203, 424
DdeI CTNAG 1 cut(s) 474
DinI GGCGCC 1 cut(s) 485
DpnI GATC 2 cut(s) 90, 211
DpnII GATC 2 cut(s) 88, 209
Eam1104I CTCTTC 2 cut(s) 23, 614
EarI CTCTTC 2 cut(s) 23, 614
Eco88I CYCGRG 1 cut(s) 362
EgeI GGCGCC 1 cut(s) 485
EheI GGCGCC 1 cut(s) 485
FaeI CATG 1 cut(s) 391
FaqI GGGAC 1 cut(s) 376
FatI CATG 1 cut(s) 387
FspBI CTAG 2 cut(s) 207, 497
GlaI GCGC 1 cut(s) 485
GsaI CCCAGC 1 cut(s) 122
HaeII RGCGCY 1 cut(s) 487
HapII CCGG 1 cut(s) 75
HhaI GCGC 1 cut(s) 486
Hin1I GRCGYC 1 cut(s) 484
Hin1II CATG 1 cut(s) 391
Hin6I GCGC 1 cut(s) 484
HinP1I GCGC 1 cut(s) 484
HincII GTYRAC 1 cut(s) 115
HindII GTYRAC 1 cut(s) 115
HinfI GANTC 4 cut(s) 317, 327, 380, 507
HpaII CCGG 1 cut(s) 75
HphI GGTGA 1 cut(s) 299
Hpy166II GTNNAC 3 cut(s) 115, 182, 203
Hpy188I TCNGA 1 cut(s) 243
Hpy188III TCNNGA 4 cut(s) 92, 362, 364, 613
Hpy8I GTNNAC 3 cut(s) 115, 182, 203
HpyAV CCTTC 2 cut(s) 20, 614
HpyCH4III ACNGT 2 cut(s) 311, 460
HpyCH4V TGCA 2 cut(s) 230, 283
HpyF3I CTNAG 1 cut(s) 474
Hsp92I GRCGYC 1 cut(s) 484
Hsp92II CATG 1 cut(s) 391
HspAI GCGC 1 cut(s) 484
KasI GGCGCC 1 cut(s) 483
Kzo9I GATC 2 cut(s) 88, 209
LpnPI CCDG 7 cut(s) 88, 132, 141, 183, 285, 509, 626
LweI GCATC 1 cut(s) 543
MaeI CTAG 2 cut(s) 207, 497
MalI GATC 2 cut(s) 90, 211
MboI GATC 2 cut(s) 88, 209
MboII GAAGA 4 cut(s) 10, 438, 476, 601
MhlI GDGCHC 1 cut(s) 144
MluCI AATT 3 cut(s) 216, 232, 291
Mly113I GGCGCC 1 cut(s) 484
MlyI GAGTC 1 cut(s) 321
MnlI CCTC 4 cut(s) 24, 270, 289, 394
MroXI GAANNNNTTC 1 cut(s) 186
MseI TTAA 1 cut(s) 266
MslI CAYNNNNRTG 3 cut(s) 55, 312, 569
MspA1I CMGCKG 1 cut(s) 155
MspI CCGG 1 cut(s) 75
MspR9I CCNGG 1 cut(s) 76
NarI GGCGCC 1 cut(s) 484
NciI CCSGG 1 cut(s) 76
NdeII GATC 2 cut(s) 88, 209
NlaIII CATG 1 cut(s) 391
NlaIV GGNNCC 2 cut(s) 485, 528
PaeR7I CTCGAG 1 cut(s) 362
PdmI GAANNNNTTC 1 cut(s) 186
PfeI GAWTC 3 cut(s) 317, 380, 507
PflFI GACNNNGTC 1 cut(s) 330
PleI GAGTC 1 cut(s) 321
PluTI GGCGCC 1 cut(s) 487
PpsI GAGTC 1 cut(s) 321
PspFI CCCAGC 1 cut(s) 118
PspN4I GGNNCC 2 cut(s) 485, 528
PsyI GACNNNGTC 1 cut(s) 330
RsaI GTAC 3 cut(s) 105, 204, 425
RsaNI GTAC 3 cut(s) 104, 203, 424
RseI CAYNNNNRTG 3 cut(s) 55, 312, 569
SaqAI TTAA 1 cut(s) 266
Sau3AI GATC 2 cut(s) 88, 209
ScaI AGTACT 1 cut(s) 105
SchI GAGTC 1 cut(s) 321
ScrFI CCNGG 1 cut(s) 76
SduI GDGCHC 1 cut(s) 144
SetI ASST 4 cut(s) 85, 208, 281, 300
SfaNI GCATC 1 cut(s) 543
SfoI GGCGCC 1 cut(s) 485
Sfr274I CTCGAG 1 cut(s) 362
SlaI CTCGAG 1 cut(s) 362
SmiMI CAYNNNNRTG 3 cut(s) 55, 312, 569
SmlI CTYRAG 1 cut(s) 362
SmoI CTYRAG 1 cut(s) 362
Sse9I AATT 3 cut(s) 216, 232, 291
SsiI CCGC 2 cut(s) 125, 153
SspDI GGCGCC 1 cut(s) 483
SspMI CTAG 2 cut(s) 207, 497
StyD4I CCNGG 1 cut(s) 74
TaaI ACNGT 2 cut(s) 311, 460
TaqI TCGA 3 cut(s) 41, 190, 363
TasI AATT 3 cut(s) 216, 232, 291
TatI WGTACW 2 cut(s) 103, 423
TfiI GAWTC 3 cut(s) 317, 380, 507
Tru1I TTAA 1 cut(s) 266
Tru9I TTAA 1 cut(s) 266
TscAI CASTG 3 cut(s) 341, 463, 507
TspDTI ATGAA 2 cut(s) 499, 539
TspRI CASTG 3 cut(s) 341, 463, 507
Tth111I GACNNNGTC 1 cut(s) 330
XapI RAATTY 1 cut(s) 232
XhoI CTCGAG 1 cut(s) 362
XmnI GAANNNNTTC 1 cut(s) 186
XspI CTAG 2 cut(s) 207, 497
ZrmI AGTACT 1 cut(s) 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.