MD12G1009700.v1.1

NEMP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0004377
Physical Location & Seq
Forward (+)
1002078 .. 1003398
1321 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1009700.v1.1.491

Sequence Viewer

Length: 705 bp
ATGTCGCACCTTACCGCTCGATGTCGCTTTGATTTTGAGTTTTTAATCTGCTGTGTAGATATTGAAAACCCAGTACTAGAAGTCATCCCAGCACCTCTTTCTGAGCACTCAGCTGTTCCTGGTTCTAAAGATGTTTTATTTTGTAAACGGGCACGAGTTTCTGGTATATCCAGGTTAAAACTTGGGAGTTATGCAAGTTCACTTAAGGTCACCTTGTCTACATCTCTAGGAATCCTGGAGAGGTTGCTTAGCAAAATTCAAGTCTGTTTTCATAGGAATAATACGCTTGGATTATGTCAGTGTGAAGAGGATGATTGGAAAAATATTCAGAAAGGGTTATGGAGCTCTGTCATGTCGCCTTATGATGAAAGATATGTCGATGTCAAGTTCATTGGCAAAATACCTGGCTCTGTCACCATAACTGTTGAAGAAGGTCTGATAAAAATGAAAGTAGATGGCGCCTTGTGTGTCTGGCACTGGGATTTACTTTACTATTGTTGGCAGCAATTGTCAGCAATGGCTATAGGAGCTTTTCTGGTCATTATAATCATTCTCTTCCAGGGAATGAAGTTGTTGTCAACTGGAAGGAAAACTGTCCTCTGTCTTACCATGTATGGATCAGTCAAACTTGGTGTCTTGAACTTATTGGCATTGCTAAACTACTACAAGATTGAAACTAGACAGGGATTAGGAGGGATTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NEMP

Protein Analysis

235

Amino Acids

26.15

Weight (kDa)

8.64

Isoelectric Point (pI)

33.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NEMP PF10225 159 - 215 2.1e-06 NEMP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 545
AccB1I GGYRCC 1 cut(s) 458
AccBSI CCGCTC 1 cut(s) 17
AccI GTMKAC 1 cut(s) 218
AciI CCGC 1 cut(s) 15
AclWI GGATC 1 cut(s) 625
AcsI RAATTY 1 cut(s) 255
AcyI GRCGYC 1 cut(s) 459
AfaI GTAC 1 cut(s) 75
AflII CTTAAG 1 cut(s) 203
AgsI TTSAA 5 cut(s) 65, 260, 428, 640, 674
AjnI CCWGG 5 cut(s) 118, 170, 234, 403, 558
AluBI AGCT 3 cut(s) 113, 345, 530
AluI AGCT 3 cut(s) 113, 345, 530
Alw21I GWGCWC 2 cut(s) 108, 347
AlwI GGATC 1 cut(s) 625
ApeKI GCWGC 1 cut(s) 502
ApoI RAATTY 1 cut(s) 255
ArsI GACNNNNNNTTYG 4 cut(s) 246, 278, 618, 650
AspLEI GCGC 1 cut(s) 461
AsuHPI GGTGA 2 cut(s) 202, 406
BaeGI GKGCMC 1 cut(s) 154
BanI GGYRCC 1 cut(s) 458
BanII GRGCYC 1 cut(s) 347
BauI CACGAG 1 cut(s) 153
Bbv12I GWGCWC 2 cut(s) 108, 347
BbvI GCAGC 1 cut(s) 514
BccI CCATC 1 cut(s) 449
BciT130I CCWGG 5 cut(s) 120, 172, 236, 405, 560
BfaI CTAG 3 cut(s) 77, 227, 678
BfmI CTRYAG 1 cut(s) 522
BfoI RGCGCY 1 cut(s) 462
BfrI CTTAAG 1 cut(s) 203
BisI GCNGC 1 cut(s) 503
BlpI GCTNAGC 1 cut(s) 248
BlsI GCNGC 1 cut(s) 504
BmcAI AGTACT 1 cut(s) 75
Bme1390I CCNGG 5 cut(s) 120, 172, 236, 405, 560
BmiI GGNNCC 1 cut(s) 460
BmrFI CCNGG 5 cut(s) 120, 172, 236, 405, 560
BmrI ACTGGG 2 cut(s) 65, 487
BmuI ACTGGG 2 cut(s) 65, 487
BpmI CTGGAG 1 cut(s) 257
Bpu1102I GCTNAGC 1 cut(s) 248
BsaHI GRCGYC 1 cut(s) 459
BsaJI CCNNGG 1 cut(s) 559
Bse1I ACTGG 3 cut(s) 71, 482, 586
Bse3DI GCAATG 2 cut(s) 522, 650
BseBI CCWGG 5 cut(s) 120, 172, 236, 405, 560
BseDI CCNNGG 1 cut(s) 559
BseGI GGATG 2 cut(s) 84, 316
BseMI GCAATG 2 cut(s) 522, 650
BseMII CTCAG 2 cut(s) 93, 123
BseNI ACTGG 3 cut(s) 71, 482, 586
BseSI GKGCMC 1 cut(s) 154
BseXI GCAGC 1 cut(s) 514
BseYI CCCAGC 1 cut(s) 88
BshNI GGYRCC 1 cut(s) 458
BsiHKAI GWGCWC 2 cut(s) 108, 347
Bsp1286I GDGCHC 3 cut(s) 108, 154, 347
Bsp143I GATC 1 cut(s) 617
Bsp1720I GCTNAGC 1 cut(s) 248
BspACI CCGC 1 cut(s) 15
BspCNI CTCAG 2 cut(s) 94, 122
BspLI GGNNCC 1 cut(s) 460
BspPI GGATC 1 cut(s) 625
BspT107I GGYRCC 1 cut(s) 458
BspTI CTTAAG 1 cut(s) 203
BsrBI CCGCTC 1 cut(s) 17
BsrDI GCAATG 2 cut(s) 522, 650
BsrI ACTGG 3 cut(s) 71, 482, 586
BssECI CCNNGG 1 cut(s) 559
BssMI GATC 1 cut(s) 617
BssNI GRCGYC 1 cut(s) 459
BssSI CACGAG 1 cut(s) 153
Bst2BI CACGAG 1 cut(s) 153
Bst2UI CCWGG 5 cut(s) 120, 172, 236, 405, 560
Bst4CI ACNGT 2 cut(s) 424, 595
Bst6I CTCTTC 2 cut(s) 300, 560
BstACI GRCGYC 1 cut(s) 459
BstAFI CTTAAG 1 cut(s) 203
BstDEI CTNAG 3 cut(s) 102, 109, 248
BstEII GGTNACC 1 cut(s) 208
BstF5I GGATG 2 cut(s) 84, 316
BstH2I RGCGCY 1 cut(s) 462
BstHHI GCGC 1 cut(s) 461
BstKTI GATC 1 cut(s) 620
BstMBI GATC 1 cut(s) 617
BstMWI GCNNNNNNNGC 1 cut(s) 527
BstNI CCWGG 5 cut(s) 120, 172, 236, 405, 560
BstPI GGTNACC 1 cut(s) 208
BstSCI CCNGG 5 cut(s) 118, 170, 234, 403, 558
BstSFI CTRYAG 1 cut(s) 522
BstSLI GKGCMC 1 cut(s) 154
BstV1I GCAGC 1 cut(s) 514
BtsCI GGATG 2 cut(s) 84, 316
BtsIMutI CAGTG 2 cut(s) 305, 475
CfoI GCGC 1 cut(s) 461
Csp6I GTAC 1 cut(s) 74
CviAII CATG 2 cut(s) 352, 610
CviJI RGCY 5 cut(s) 113, 345, 408, 521, 530
CviKI_1 RGCY 5 cut(s) 113, 345, 408, 521, 530
CviQI GTAC 1 cut(s) 74
DdeI CTNAG 3 cut(s) 102, 109, 248
DinI GGCGCC 1 cut(s) 460
DpnI GATC 1 cut(s) 619
DpnII GATC 1 cut(s) 617
Eam1104I CTCTTC 2 cut(s) 300, 560
EarI CTCTTC 2 cut(s) 300, 560
Ecl136II GAGCTC 1 cut(s) 345
Eco24I GRGCYC 1 cut(s) 347
Eco53kI GAGCTC 1 cut(s) 345
Eco91I GGTNACC 1 cut(s) 208
EcoICRI GAGCTC 1 cut(s) 345
EcoO65I GGTNACC 1 cut(s) 208
EcoRII CCWGG 5 cut(s) 118, 170, 234, 403, 558
EcoT38I GRGCYC 1 cut(s) 347
EgeI GGCGCC 1 cut(s) 460
EheI GGCGCC 1 cut(s) 460
FaeI CATG 2 cut(s) 355, 613
FalI AAGNNNNNCTT 2 cut(s) 197, 229
FatI CATG 2 cut(s) 351, 609
FblI GTMKAC 1 cut(s) 218
Fnu4HI GCNGC 1 cut(s) 503
FokI GGATG 2 cut(s) 71, 323
FriOI GRGCYC 1 cut(s) 347
Fsp4HI GCNGC 1 cut(s) 503
FspBI CTAG 3 cut(s) 77, 227, 678
GlaI GCGC 1 cut(s) 460
GluI GCNGC 1 cut(s) 503
GsaI CCCAGC 1 cut(s) 92
GsuI CTGGAG 1 cut(s) 257
HaeII RGCGCY 1 cut(s) 462
HhaI GCGC 1 cut(s) 461
Hin1I GRCGYC 1 cut(s) 459
Hin1II CATG 2 cut(s) 355, 613
Hin6I GCGC 1 cut(s) 459
HinP1I GCGC 1 cut(s) 459
HincII GTYRAC 1 cut(s) 579
HindII GTYRAC 1 cut(s) 579
HinfI GANTC 1 cut(s) 231
HphI GGTGA 2 cut(s) 202, 406
Hpy166II GTNNAC 4 cut(s) 146, 200, 219, 579
Hpy188I TCNGA 3 cut(s) 103, 330, 438
Hpy188III TCNNGA 1 cut(s) 637
Hpy8I GTNNAC 4 cut(s) 146, 200, 219, 579
HpyAV CCTTC 2 cut(s) 425, 579
HpyCH4III ACNGT 2 cut(s) 424, 595
HpyCH4V TGCA 1 cut(s) 194
HpyF10VI GCNNNNNNNGC 1 cut(s) 527
HpyF3I CTNAG 3 cut(s) 102, 109, 248
Hsp92I GRCGYC 1 cut(s) 459
Hsp92II CATG 2 cut(s) 355, 613
HspAI GCGC 1 cut(s) 459
KasI GGCGCC 1 cut(s) 458
Kzo9I GATC 1 cut(s) 617
LmnI GCTCC 2 cut(s) 342, 527
Lsp1109I GCAGC 1 cut(s) 514
MaeI CTAG 3 cut(s) 77, 227, 678
MaeIII GTNAC 2 cut(s) 208, 412
MalI GATC 1 cut(s) 619
MbiI CCGCTC 1 cut(s) 17
MboI GATC 1 cut(s) 617
MboII GAAGA 3 cut(s) 317, 440, 547
MfeI CAATTG 1 cut(s) 506
MhlI GDGCHC 3 cut(s) 108, 154, 347
MluCI AATT 2 cut(s) 255, 506
Mly113I GGCGCC 1 cut(s) 459
MnlI CCTC 5 cut(s) 105, 234, 301, 608, 686
MseI TTAA 4 cut(s) 44, 176, 204, 703
MspA1I CMGCKG 1 cut(s) 113
MspCI CTTAAG 1 cut(s) 203
MspR9I CCNGG 5 cut(s) 120, 172, 236, 405, 560
MunI CAATTG 1 cut(s) 506
MvaI CCWGG 5 cut(s) 120, 172, 236, 405, 560
MwoI GCNNNNNNNGC 1 cut(s) 527
NarI GGCGCC 1 cut(s) 459
NdeII GATC 1 cut(s) 617
NlaIII CATG 2 cut(s) 355, 613
NlaIV GGNNCC 1 cut(s) 460
NmuCI GTSAC 2 cut(s) 208, 412
PfeI GAWTC 1 cut(s) 231
PfoI TCCNGGA 1 cut(s) 234
PkrI GCNGC 1 cut(s) 504
PluTI GGCGCC 1 cut(s) 462
PsiI TTATAA 1 cut(s) 545
Psp124BI GAGCTC 1 cut(s) 347
Psp6I CCWGG 5 cut(s) 118, 170, 234, 403, 558
PspEI GGTNACC 1 cut(s) 208
PspFI CCCAGC 1 cut(s) 88
PspGI CCWGG 5 cut(s) 118, 170, 234, 403, 558
PspN4I GGNNCC 1 cut(s) 460
PvuII CAGCTG 1 cut(s) 113
RsaI GTAC 1 cut(s) 75
RsaNI GTAC 1 cut(s) 74
SacI GAGCTC 1 cut(s) 347
SaqAI TTAA 4 cut(s) 44, 176, 204, 703
SatI GCNGC 1 cut(s) 503
Sau3AI GATC 1 cut(s) 617
ScaI AGTACT 1 cut(s) 75
ScrFI CCNGG 5 cut(s) 120, 172, 236, 405, 560
SduI GDGCHC 3 cut(s) 108, 154, 347
SfcI CTRYAG 1 cut(s) 522
SfoI GGCGCC 1 cut(s) 460
SmlI CTYRAG 1 cut(s) 203
SmoI CTYRAG 1 cut(s) 203
Sse9I AATT 2 cut(s) 255, 506
SsiI CCGC 1 cut(s) 15
SspDI GGCGCC 1 cut(s) 458
SspI AATATT 1 cut(s) 325
SspMI CTAG 3 cut(s) 77, 227, 678
SstI GAGCTC 1 cut(s) 347
StyD4I CCNGG 5 cut(s) 118, 170, 234, 403, 558
TaaI ACNGT 2 cut(s) 424, 595
TaqI TCGA 2 cut(s) 19, 378
TasI AATT 2 cut(s) 255, 506
TatI WGTACW 1 cut(s) 73
TfiI GAWTC 1 cut(s) 231
Tru1I TTAA 4 cut(s) 44, 176, 204, 703
Tru9I TTAA 4 cut(s) 44, 176, 204, 703
TscAI CASTG 2 cut(s) 305, 482
TseFI GTSAC 2 cut(s) 208, 412
TseI GCWGC 1 cut(s) 502
Tsp45I GTSAC 2 cut(s) 208, 412
TspDTI ATGAA 5 cut(s) 260, 379, 381, 461, 581
TspRI CASTG 2 cut(s) 305, 482
Vha464I CTTAAG 1 cut(s) 203
XapI RAATTY 1 cut(s) 255
XmiI GTMKAC 1 cut(s) 218
XspI CTAG 3 cut(s) 77, 227, 678
ZrmI AGTACT 1 cut(s) 75
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.