Rroxscaffold_6G00389440

NEMP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0004377
Physical Location & Seq
Forward (+)
2968609 .. 2976204
7596 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00389440.1

Sequence Viewer

Length: 1200 bp
ATGTTCCACTACCCCTTCCCCTACCCCTACCGGGAAAGCAGACTAGAGGGTATTGATCTTGAAAATCCAGTACTAAATGTCAACCCAGCACCGCTTTCTCTTTCTGGGCACTCGCCTACCGCTGGTTCTAAAGATGTGTTATTATTTTGTGAGCGGGTTCGAGTTTCTGGTATGCCTAGATCAAAAATTGGGAGTTATGCAAATTCCTTTCGGATTACTCTGTCTCCATCTGTTGTAATCCCAGAGAGGTTGCATAGCAAAATTGAGGTCTGTTTTCACCGTAATGATTCGCTTGGACTCTGTCAGTGTAGAAATGATGAGTGGAAAACTCTCGAGAAAGGGCTATGGGAATCTGTCATGTCCCCTTATGAGGAGAAATATGTTGATGTCAAGTACATTGGCAATTTACTTGGGTCTTCCACCATCACTGTTGAAGAAGTTTCTCAGCAATGGCGCCTTTGTAACAAGGATGCTGTAATTTTAGAGATCTCTAAATTTTTACTGGTGGCGATTATCCTTATGGGTGCTGCACTAGGTTACTGGATTGGGAGGAAGCTTTTCATCTCAAAGGATGGAACAGTAGATGTTGTTGTAGCCCAATTTTCTATGTGGGCAATCCGCTTTTTTGGAATCACGTTCATCTCTCAGAGCACTCTCGATACTCCTTTGGCTATTGGAGCATTGGTTTCCTGCTTGGTTATCTGCAAACTTATCACTTCTGTGAAGTGGCATGGTAGAATGAATCAGTCAAATGGTGCGAGTGGGAGTCCTTTACAGCAGAAAGGAAGACACGTCACAGGAAGACACAGTCGTCCAGAGTTCCTTAGCAGGTCCAGCGCACTAGAGGCATGGAACAGTACAAGGGGTGCATCTGCTTGGTCTGATTCTCCTGTTAAAGGTGTTGTGTCGCCATCTTTTAGGACATGTGATCAACAGGAGTACTATTCAACCTTCCACAAGATGCGAAACCGAAAGAAGTTCACAAAGAAAGAGTGGGATGATTTTACAATGGAATCAACACGGCATGCTCTAGCAGAATGGGCAGCATCTCCTGAATTCACCGATTGGGTTATTGAGCATGCCGACCGCATACAACTCCTTCCAAGCGATAGTTCAGATGAAACTGTGGGAAGTGAATCTGATTCTACTGATGAGAATGTCGTGGGGAGCATGGATAGGTGTGGTTTTCTCAAATGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NEMP

Protein Analysis

399

Amino Acids

44.94

Weight (kDa)

7.13

Isoelectric Point (pI)

46.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NEMP PF10225 136 - 318 1.3e-11 NEMP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 810
Acc36I ACCTGC 1 cut(s) 819
AccB1I GGYRCC 1 cut(s) 453
AccBSI CCGCTC 1 cut(s) 154
AciI CCGC 5 cut(s) 92, 120, 154, 619, 1087
AcsI RAATTY 3 cut(s) 202, 494, 1055
AcyI GRCGYC 1 cut(s) 454
AfaI GTAC 4 cut(s) 72, 395, 859, 941
AfiI CCNNNNNNNGG 4 cut(s) 31, 122, 370, 896
AflIII ACRYGT 2 cut(s) 790, 923
AgsI TTSAA 3 cut(s) 62, 434, 948
AjiI CACGTC 1 cut(s) 793
AjuI GAANNNNNNNTTGG 2 cut(s) 1096, 1128
AleI CACNNNNGTG 1 cut(s) 719
AluBI AGCT 1 cut(s) 556
AluI AGCT 1 cut(s) 556
Alw21I GWGCWC 1 cut(s) 653
Alw26I GTCTC 1 cut(s) 228
Ama87I CYCGRG 1 cut(s) 332
ApeKI GCWGC 2 cut(s) 527, 1043
ApoI RAATTY 3 cut(s) 202, 494, 1055
ArsI GACNNNNNNTTYG 2 cut(s) 252, 284
Asp700I GAANNNNTTC 1 cut(s) 557
AspLEI GCGC 2 cut(s) 456, 839
AspS9I GGNCC 1 cut(s) 831
AsuC2I CCSGG 1 cut(s) 32
AsuHPI GGTGA 2 cut(s) 269, 1051
AvaI CYCGRG 1 cut(s) 332
AvaII GGWCC 1 cut(s) 831
BaeGI GKGCMC 1 cut(s) 111
BanI GGYRCC 1 cut(s) 453
BbsI GAAGAC 3 cut(s) 408, 793, 808
Bbv12I GWGCWC 1 cut(s) 653
BbvI GCAGC 2 cut(s) 514, 1055
BccI CCATC 4 cut(s) 235, 431, 566, 919
BceAI ACGGC 1 cut(s) 1037
BclI TGATCA 1 cut(s) 928
BcnI CCSGG 1 cut(s) 32
BcoDI GTCTC 1 cut(s) 228
BfaI CTAG 5 cut(s) 44, 177, 533, 842, 1031
BfoI RGCGCY 1 cut(s) 457
BfuAI ACCTGC 1 cut(s) 819
BglII AGATCT 1 cut(s) 486
BisI GCNGC 2 cut(s) 528, 1044
BlsI GCNGC 2 cut(s) 529, 1045
BmcAI AGTACT 2 cut(s) 72, 941
Bme1390I CCNGG 1 cut(s) 32
Bme18I GGWCC 1 cut(s) 831
BmeT110I CYCGRG 1 cut(s) 332
BmgBI CACGTC 1 cut(s) 793
BmgT120I GGNCC 1 cut(s) 831
BmiI GGNNCC 1 cut(s) 455
BmrFI CCNGG 1 cut(s) 32
BmsI GCATC 4 cut(s) 460, 878, 951, 1055
BpiI GAAGAC 3 cut(s) 408, 793, 808
Bpu10I CCTNAGC 1 cut(s) 824
BpuMI CCSGG 1 cut(s) 32
BsaHI GRCGYC 1 cut(s) 454
BsaXI ACNNNNNCTCC 4 cut(s) 208, 238, 669, 699
Bsc4I CCNNNNNNNGG 4 cut(s) 31, 122, 370, 896
Bse1I ACTGG 3 cut(s) 68, 507, 545
Bse3DI GCAATG 1 cut(s) 455
BseGI GGATG 3 cut(s) 475, 577, 1003
BseLI CCNNNNNNNGG 4 cut(s) 31, 122, 370, 896
BseMI GCAATG 1 cut(s) 455
BseMII CTCAG 2 cut(s) 458, 659
BseNI ACTGG 3 cut(s) 68, 507, 545
BseRI GAGGAG 1 cut(s) 386
BseSI GKGCMC 1 cut(s) 111
BseXI GCAGC 2 cut(s) 514, 1055
BseYI CCCAGC 1 cut(s) 85
BsgI GTGCAG 1 cut(s) 513
Bsh1285I CGRYCG 1 cut(s) 1087
BshNI GGYRCC 1 cut(s) 453
BsiEI CGRYCG 1 cut(s) 1087
BsiHKAI GWGCWC 1 cut(s) 653
BsiHKCI CYCGRG 1 cut(s) 332
BsiSI CCGG 1 cut(s) 31
BslFI GGGAC 1 cut(s) 346
BslI CCNNNNNNNGG 4 cut(s) 31, 122, 370, 896
BsmAI GTCTC 1 cut(s) 228
BsmFI GGGAC 1 cut(s) 346
BsoBI CYCGRG 1 cut(s) 332
Bsp1286I GDGCHC 2 cut(s) 111, 653
Bsp143I GATC 4 cut(s) 55, 179, 486, 928
BspACI CCGC 5 cut(s) 92, 120, 154, 619, 1087
BspCNI CTCAG 2 cut(s) 457, 658
BspLI GGNNCC 1 cut(s) 455
BspMI ACCTGC 1 cut(s) 819
BspT107I GGYRCC 1 cut(s) 453
BsrBI CCGCTC 1 cut(s) 154
BsrDI GCAATG 1 cut(s) 455
BsrI ACTGG 3 cut(s) 68, 507, 545
BssMI GATC 4 cut(s) 55, 179, 486, 928
BssNI GRCGYC 1 cut(s) 454
Bst4CI ACNGT 6 cut(s) 281, 430, 580, 809, 857, 1126
BstACI GRCGYC 1 cut(s) 454
BstC8I GCNNGC 2 cut(s) 1026, 1080
BstDEI CTNAG 3 cut(s) 444, 645, 824
BstENI CCTNNNNNAGG 1 cut(s) 894
BstF5I GGATG 3 cut(s) 475, 577, 1003
BstH2I RGCGCY 1 cut(s) 457
BstHHI GCGC 2 cut(s) 456, 839
BstKTI GATC 4 cut(s) 58, 182, 489, 931
BstMAI GTCTC 1 cut(s) 228
BstMBI GATC 4 cut(s) 55, 179, 486, 928
BstMCI CGRYCG 1 cut(s) 1087
BstMWI GCNNNNNNNGC 4 cut(s) 677, 834, 845, 1040
BstNSI RCATGY 3 cut(s) 927, 1028, 1082
BstSCI CCNGG 1 cut(s) 30
BstSLI GKGCMC 1 cut(s) 111
BstV1I GCAGC 2 cut(s) 514, 1055
BstV2I GAAGAC 3 cut(s) 408, 793, 808
BstX2I RGATCY 1 cut(s) 486
BstYI RGATCY 1 cut(s) 486
BtrI CACGTC 1 cut(s) 793
BtsCI GGATG 3 cut(s) 475, 577, 1003
BtsIMutI CAGTG 2 cut(s) 311, 426
BveI ACCTGC 1 cut(s) 819
Cac8I GCNNGC 2 cut(s) 1026, 1080
CfoI GCGC 2 cut(s) 456, 839
Cfr13I GGNCC 1 cut(s) 831
Csp6I GTAC 4 cut(s) 71, 394, 858, 940
CviAII CATG 7 cut(s) 358, 731, 849, 924, 1025, 1079, 1171
CviJI RGCY 4 cut(s) 343, 556, 596, 671
CviKI_1 RGCY 4 cut(s) 343, 556, 596, 671
CviQI GTAC 4 cut(s) 71, 394, 858, 940
DdeI CTNAG 3 cut(s) 444, 645, 824
DinI GGCGCC 1 cut(s) 455
DpnI GATC 4 cut(s) 57, 181, 488, 930
DpnII GATC 4 cut(s) 55, 179, 486, 928
DrdI GACNNNNNNGTC 1 cut(s) 810
DseDI GACNNNNNNGTC 1 cut(s) 810
Eco47I GGWCC 1 cut(s) 831
Eco88I CYCGRG 1 cut(s) 332
EcoNI CCTNNNNNAGG 1 cut(s) 894
EcoRI GAATTC 1 cut(s) 1055
EgeI GGCGCC 1 cut(s) 455
EheI GGCGCC 1 cut(s) 455
FaeI CATG 7 cut(s) 361, 734, 852, 927, 1028, 1082, 1174
FaqI GGGAC 1 cut(s) 346
FatI CATG 7 cut(s) 357, 730, 848, 923, 1024, 1078, 1170
FauI CCCGC 1 cut(s) 147
FbaI TGATCA 1 cut(s) 928
Fnu4HI GCNGC 2 cut(s) 528, 1044
FokI GGATG 3 cut(s) 482, 584, 1010
Fsp4HI GCNGC 2 cut(s) 528, 1044
FspBI CTAG 5 cut(s) 44, 177, 533, 842, 1031
GlaI GCGC 2 cut(s) 455, 838
GluI GCNGC 2 cut(s) 528, 1044
GsaI CCCAGC 1 cut(s) 89
HaeII RGCGCY 1 cut(s) 457
HapII CCGG 1 cut(s) 31
HhaI GCGC 2 cut(s) 456, 839
Hin1I GRCGYC 1 cut(s) 454
Hin1II CATG 7 cut(s) 361, 734, 852, 927, 1028, 1082, 1174
Hin6I GCGC 2 cut(s) 454, 837
HinP1I GCGC 2 cut(s) 454, 837
HincII GTYRAC 1 cut(s) 82
HindII GTYRAC 1 cut(s) 82
HindIII AAGCTT 1 cut(s) 554
HpaII CCGG 1 cut(s) 31
HphI GGTGA 2 cut(s) 269, 1051
Hpy166II GTNNAC 2 cut(s) 82, 981
Hpy188I TCNGA 5 cut(s) 213, 648, 883, 1117, 1141
Hpy188III TCNNGA 6 cut(s) 59, 332, 334, 656, 815, 1052
Hpy8I GTNNAC 2 cut(s) 82, 981
HpyAV CCTTC 3 cut(s) 25, 961, 1109
HpyCH4III ACNGT 6 cut(s) 281, 430, 580, 809, 857, 1126
HpyCH4IV ACGT 2 cut(s) 635, 792
HpyCH4V TGCA 5 cut(s) 200, 253, 530, 705, 869
HpyF10VI GCNNNNNNNGC 4 cut(s) 677, 834, 845, 1040
HpyF3I CTNAG 3 cut(s) 444, 645, 824
HpySE526I ACGT 2 cut(s) 635, 792
Hsp92I GRCGYC 1 cut(s) 454
Hsp92II CATG 7 cut(s) 361, 734, 852, 927, 1028, 1082, 1174
HspAI GCGC 2 cut(s) 454, 837
KasI GGCGCC 1 cut(s) 453
Ksp22I TGATCA 1 cut(s) 928
Kzo9I GATC 4 cut(s) 55, 179, 486, 928
LmnI GCTCC 2 cut(s) 677, 1167
Lsp1109I GCAGC 2 cut(s) 514, 1055
LweI GCATC 4 cut(s) 460, 878, 951, 1055
MaeI CTAG 5 cut(s) 44, 177, 533, 842, 1031
MaeII ACGT 2 cut(s) 635, 792
MaeIII GTNAC 3 cut(s) 461, 536, 793
MalI GATC 4 cut(s) 57, 181, 488, 930
MbiI CCGCTC 1 cut(s) 154
MboI GATC 4 cut(s) 55, 179, 486, 928
MboII GAAGA 4 cut(s) 408, 446, 798, 813
MflI RGATCY 1 cut(s) 486
MhlI GDGCHC 2 cut(s) 111, 653
MluCI AATT 8 cut(s) 186, 202, 261, 403, 477, 494, 599, 1055
Mly113I GGCGCC 1 cut(s) 454
MlyI GAGTC 2 cut(s) 291, 775
MnlI CCTC 6 cut(s) 40, 240, 259, 364, 543, 838
MroXI GAANNNNTTC 1 cut(s) 557
MseI TTAA 1 cut(s) 894
MslI CAYNNNNRTG 2 cut(s) 282, 719
MspA1I CMGCKG 1 cut(s) 122
MspI CCGG 1 cut(s) 31
MspR9I CCNGG 1 cut(s) 32
MwoI GCNNNNNNNGC 4 cut(s) 677, 834, 845, 1040
NarI GGCGCC 1 cut(s) 454
NciI CCSGG 1 cut(s) 32
NdeII GATC 4 cut(s) 55, 179, 486, 928
NlaIII CATG 7 cut(s) 361, 734, 852, 927, 1028, 1082, 1174
NlaIV GGNNCC 1 cut(s) 455
NmuCI GTSAC 1 cut(s) 793
NspI RCATGY 3 cut(s) 927, 1028, 1082
OliI CACNNNNGTG 1 cut(s) 719
PaeI GCATGC 2 cut(s) 1028, 1082
PaeR7I CTCGAG 1 cut(s) 332
PciI ACATGT 1 cut(s) 923
PdmI GAANNNNTTC 1 cut(s) 557
PfeI GAWTC 8 cut(s) 287, 350, 630, 742, 884, 1013, 1136, 1142
PflFI GACNNNGTC 2 cut(s) 300, 807
PkrI GCNGC 2 cut(s) 529, 1045
PleI GAGTC 2 cut(s) 291, 774
PluTI GGCGCC 1 cut(s) 457
PpsI GAGTC 2 cut(s) 291, 774
PscI ACATGT 1 cut(s) 923
PspFI CCCAGC 1 cut(s) 85
PspN4I GGNNCC 1 cut(s) 455
PspPI GGNCC 1 cut(s) 831
PsuI RGATCY 1 cut(s) 486
PsyI GACNNNGTC 2 cut(s) 300, 807
RsaI GTAC 4 cut(s) 72, 395, 859, 941
RsaNI GTAC 4 cut(s) 71, 394, 858, 940
RseI CAYNNNNRTG 2 cut(s) 282, 719
SaqAI TTAA 1 cut(s) 894
SatI GCNGC 2 cut(s) 528, 1044
Sau3AI GATC 4 cut(s) 55, 179, 486, 928
Sau96I GGNCC 1 cut(s) 831
ScaI AGTACT 2 cut(s) 72, 941
SchI GAGTC 2 cut(s) 291, 775
ScrFI CCNGG 1 cut(s) 32
SduI GDGCHC 2 cut(s) 111, 653
SfaNI GCATC 4 cut(s) 460, 878, 951, 1055
SfoI GGCGCC 1 cut(s) 455
Sfr274I CTCGAG 1 cut(s) 332
SinI GGWCC 1 cut(s) 831
SlaI CTCGAG 1 cut(s) 332
SmiMI CAYNNNNRTG 2 cut(s) 282, 719
SmlI CTYRAG 1 cut(s) 332
SmoI CTYRAG 1 cut(s) 332
SphI GCATGC 2 cut(s) 1028, 1082
Sse9I AATT 8 cut(s) 186, 202, 261, 403, 477, 494, 599, 1055
SsiI CCGC 5 cut(s) 92, 120, 154, 619, 1087
SspDI GGCGCC 1 cut(s) 453
SspMI CTAG 5 cut(s) 44, 177, 533, 842, 1031
StyD4I CCNGG 1 cut(s) 30
TaaI ACNGT 6 cut(s) 281, 430, 580, 809, 857, 1126
TaiI ACGT 2 cut(s) 638, 795
TaqI TCGA 3 cut(s) 160, 333, 657
TasI AATT 8 cut(s) 186, 202, 261, 403, 477, 494, 599, 1055
TatI WGTACW 4 cut(s) 70, 393, 857, 939
TfiI GAWTC 8 cut(s) 287, 350, 630, 742, 884, 1013, 1136, 1142
Tru1I TTAA 1 cut(s) 894
Tru9I TTAA 1 cut(s) 894
TscAI CASTG 2 cut(s) 311, 433
TseFI GTSAC 1 cut(s) 793
TseI GCWGC 2 cut(s) 527, 1043
Tsp45I GTSAC 1 cut(s) 793
TspDTI ATGAA 4 cut(s) 550, 628, 755, 1134
TspRI CASTG 2 cut(s) 311, 433
Tth111I GACNNNGTC 2 cut(s) 300, 807
VpaK11BI GGWCC 1 cut(s) 831
XagI CCTNNNNNAGG 1 cut(s) 894
XapI RAATTY 3 cut(s) 202, 494, 1055
XceI RCATGY 3 cut(s) 927, 1028, 1082
XhoI CTCGAG 1 cut(s) 332
XmnI GAANNNNTTC 1 cut(s) 557
XspI CTAG 5 cut(s) 44, 177, 533, 842, 1031
ZrmI AGTACT 2 cut(s) 72, 941
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.