MD14G1007100.v1.1

NEMP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0004377
Physical Location & Seq
Forward (+)
752191 .. 756147
3957 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1007100.v1.1.491

Sequence Viewer

Length: 1494 bp
ATGGGCTCTTCTCCCTCCTCCGCCACTCTCTTCCTCCTCTTCGCTTTCTGTCTCTCTCTTCCGACCCTCTCACCTGCCACCGACCTCAAAGGTGTTGATATTGAAAACCCAGTACTAGAAGTCATCCCGGCACCTCTTTCCGAGCACTCAGATGTGCCTGGTTCTAAAGATGTTTTATTCTGTGAACGGGCACGAGTTTCTGGGGTATCAAGGTTAAAACTTGGGAGTTATGCAAGTTCACTTAAGATCACTTTGTCTACATCTCTTGGAATCCCAGAGAGCTTGCATAACAAAATTCAAGTCTGTTTTCATAGGAATAATACGCTTGGATTATGTCAGTGCGAAGAGGATGATTGGAAAAATCTTCAGAAAGGGTTATGGAGCTCTGTCATGTCGCCTTACGATGAAAAATATGTCGATGTCAAGTTCATTGGCAAAATACCTGGCTCTGTCACCATAACTGTTGAAGAAGATTTTCAGAGATGGCGCCTTGTGTTTCTGGCATTGGGATTCACATTTCTATTGTTGGCACCAATTGTCAGCAGTTGGGTACCTTTTTATTATAGCAGTTCAATGATTATAGGAGTATTTCTGGTCATTATAATCATTCTCTTCCAGGGAATGAAGCTGTTGCCAACTGGAAGGAAAAATGTCCTCTATCTTACCATATATGGATCAGTGCTTGGAGCAGGATCTTTCCTTGTTCATCAGTTTTCACTGATGGTAAATTCAATTCTTTTGAATTTTGGGCTAAGTGAGGAGATGCATAACCCAGTCTCAATATTTTTACTAGTGGGAATTATCCTTGCGGGAGCTGCATTAGGGTACTGGATTGTGAGGAAGTTTGTCGTCTCAAATGATGGGACAGTAGATGTTGGTATAGCACAATTTGTCAAGTGGGCTATGCGTATCATTGGAATCACCTCCATCTTGCAGAGCTCACTTGATACTCCTTTAGCAATGGGCGCTTTGGTTTCTTACTGGATTATCTGCAAGTTCTTTACTTCTCTGAAACGGCGTCTCAAATCTCATCAGTCGGGTGCTGGGAGTGGGAGTCTTTGGCTGCAGAAGGGAAAACAGGTCAAGGGTAGACAAAGTCGTCCAGAATTTCTTAGCAGGTCTAGCCCACAAGAAAAGAGTTGGAACAGCCGAAAGAGTCTATCTGCTTGGTCTGACTCTCCTGTTAAAGGTGTCATGACCCCATCCTCCAGGGCACCTAATCAACAGGAGTACTATTCAACCTTCCACGAGGTGCAGAACCGAAAGAAGTTCACAAAAAAAGAGTGGGATGATTTCACACACGAATCAACCCGCCAAGCTCTAGCAGAATGGGCAGCATCTCCTGAAGTCCCTAATTGGCTCATTGAGCATGCTGACCGCATACAGCTCCTTCCAAGCGAGAGTTCAGATGAAACTGTGGGAAGCGCATCAGATTCTACGGATGAGAATGTCGTGGGGAGCACAGATCGATTAGGTTTTCTTAAGTGGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NEMP

Protein Analysis

498

Amino Acids

55.21

Weight (kDa)

8.57

Isoelectric Point (pI)

45.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NEMP PF10225 152 - 415 3.5e-49 NEMP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 602
AarI CACCTGC 1 cut(s) 82
AasI GACNNNNNNGTC 1 cut(s) 1098
Acc36I ACCTGC 2 cut(s) 82, 1107
Acc65I GGTACC 1 cut(s) 550
AccB1I GGYRCC 5 cut(s) 130, 486, 529, 550, 1213
AccI GTMKAC 2 cut(s) 257, 1090
AciI CCGC 4 cut(s) 21, 809, 1312, 1378
AclWI GGATC 2 cut(s) 682, 700
AcsI RAATTY 4 cut(s) 294, 727, 742, 1106
AcuI CTGAAG 2 cut(s) 350, 1365
AcyI GRCGYC 2 cut(s) 487, 1018
AdeI CACNNNGTG 1 cut(s) 1252
AfaI GTAC 4 cut(s) 114, 552, 827, 1232
AfiI CCNNNNNNNGG 1 cut(s) 1187
AflII CTTAAG 2 cut(s) 242, 1481
AgsI TTSAA 7 cut(s) 104, 299, 467, 573, 732, 742, 1239
AhlI ACTAGT 1 cut(s) 790
AjnI CCWGG 4 cut(s) 157, 442, 615, 1208
AjuI GAANNNNNNNTTGG 2 cut(s) 1387, 1419
AluBI AGCT 7 cut(s) 282, 384, 628, 815, 939, 1319, 1387
AluI AGCT 7 cut(s) 282, 384, 628, 815, 939, 1319, 1387
Alw21I GWGCWC 4 cut(s) 147, 386, 941, 1463
Alw26I GTCTC 4 cut(s) 56, 781, 856, 1025
AlwI GGATC 2 cut(s) 682, 700
ApeKI GCWGC 3 cut(s) 815, 1063, 1334
ApoI RAATTY 4 cut(s) 294, 727, 742, 1106
ArsI GACNNNNNNTTYG 2 cut(s) 285, 317
Asp700I GAANNNNTTC 1 cut(s) 474
Asp718I GGTACC 1 cut(s) 550
AspLEI GCGC 3 cut(s) 489, 968, 1427
AsuC2I CCSGG 1 cut(s) 128
AsuHPI GGTGA 3 cut(s) 63, 445, 913
BaeGI GKGCMC 2 cut(s) 193, 1216
BanI GGYRCC 5 cut(s) 130, 486, 529, 550, 1213
BanII GRGCYC 3 cut(s) 8, 386, 941
BauI CACGAG 2 cut(s) 192, 1247
Bbv12I GWGCWC 4 cut(s) 147, 386, 941, 1463
BbvI GCAGC 3 cut(s) 802, 1050, 1346
BccI CCATC 5 cut(s) 477, 715, 854, 935, 1210
BceAI ACGGC 1 cut(s) 1031
BciT130I CCWGG 4 cut(s) 159, 444, 617, 1210
BcnI CCSGG 1 cut(s) 128
BcoDI GTCTC 4 cut(s) 56, 781, 856, 1025
BcuI ACTAGT 1 cut(s) 790
BfaI CTAG 4 cut(s) 116, 791, 1122, 1322
BfmI CTRYAG 1 cut(s) 1064
BfoI RGCGCY 2 cut(s) 490, 969
BfrI CTTAAG 2 cut(s) 242, 1481
BfuAI ACCTGC 2 cut(s) 82, 1107
BisI GCNGC 3 cut(s) 816, 1064, 1335
BlsI GCNGC 3 cut(s) 817, 1065, 1336
BmcAI AGTACT 2 cut(s) 114, 1232
Bme1390I CCNGG 5 cut(s) 128, 159, 444, 617, 1210
BmiI GGNNCC 5 cut(s) 132, 488, 531, 552, 1215
BmrFI CCNGG 5 cut(s) 128, 159, 444, 617, 1210
BmrI ACTGGG 2 cut(s) 104, 767
BmsI GCATC 3 cut(s) 753, 1346, 1436
BmuI ACTGGG 2 cut(s) 104, 767
BpmI CTGGAG 1 cut(s) 1192
BpuMI CCSGG 1 cut(s) 128
Bsa29I ATCGAT 1 cut(s) 1468
BsaHI GRCGYC 2 cut(s) 487, 1018
BsaJI CCNNGG 2 cut(s) 616, 1209
BsaXI ACNNNNNCTCC 2 cut(s) 1039, 1069
Bsc4I CCNNNNNNNGG 1 cut(s) 1187
Bse1I ACTGG 5 cut(s) 110, 643, 773, 833, 986
Bse3DI GCAATG 1 cut(s) 966
BseBI CCWGG 4 cut(s) 159, 444, 617, 1210
BseCI ATCGAT 1 cut(s) 1468
BseDI CCNNGG 2 cut(s) 616, 1209
BseGI GGATG 5 cut(s) 123, 355, 1202, 1294, 1447
BseLI CCNNNNNNNGG 1 cut(s) 1187
BseMI GCAATG 1 cut(s) 966
BseMII CTCAG 1 cut(s) 162
BseNI ACTGG 5 cut(s) 110, 643, 773, 833, 986
BseRI GAGGAG 3 cut(s) 7, 26, 773
BseSI GKGCMC 2 cut(s) 193, 1216
BseXI GCAGC 3 cut(s) 802, 1050, 1346
BseYI CCCAGC 1 cut(s) 1043
BsgI GTGCAG 1 cut(s) 1274
BshNI GGYRCC 5 cut(s) 130, 486, 529, 550, 1213
BshVI ATCGAT 1 cut(s) 1468
BsiHKAI GWGCWC 4 cut(s) 147, 386, 941, 1463
BsiSI CCGG 1 cut(s) 128
BslFI GGGAC 2 cut(s) 877, 1334
BslI CCNNNNNNNGG 1 cut(s) 1187
BsmAI GTCTC 4 cut(s) 56, 781, 856, 1025
BsmBI CGTCTC 2 cut(s) 856, 1025
BsmFI GGGAC 2 cut(s) 877, 1334
Bsp1286I GDGCHC 7 cut(s) 8, 147, 193, 386, 941, 1216, 1463
Bsp143I GATC 4 cut(s) 246, 674, 692, 1465
BspACI CCGC 4 cut(s) 21, 809, 1312, 1378
BspCNI CTCAG 1 cut(s) 161
BspDI ATCGAT 1 cut(s) 1468
BspHI TCATGA 1 cut(s) 1194
BspLI GGNNCC 5 cut(s) 132, 488, 531, 552, 1215
BspMAI CTGCAG 1 cut(s) 1068
BspMI ACCTGC 2 cut(s) 82, 1107
BspPI GGATC 2 cut(s) 682, 700
BspQI GCTCTTC 1 cut(s) 13
BspT107I GGYRCC 5 cut(s) 130, 486, 529, 550, 1213
BspTI CTTAAG 2 cut(s) 242, 1481
BsrDI GCAATG 1 cut(s) 966
BsrI ACTGG 5 cut(s) 110, 643, 773, 833, 986
BssECI CCNNGG 2 cut(s) 616, 1209
BssMI GATC 4 cut(s) 246, 674, 692, 1465
BssNI GRCGYC 2 cut(s) 487, 1018
BssSI CACGAG 2 cut(s) 192, 1247
Bst2BI CACGAG 2 cut(s) 192, 1247
Bst2UI CCWGG 4 cut(s) 159, 444, 617, 1210
Bst4CI ACNGT 3 cut(s) 463, 868, 1417
Bst6I CTCTTC 6 cut(s) 13, 35, 44, 63, 339, 617
BstACI GRCGYC 2 cut(s) 487, 1018
BstAFI CTTAAG 2 cut(s) 242, 1481
BstC8I GCNNGC 2 cut(s) 284, 1371
BstDEI CTNAG 3 cut(s) 148, 752, 1112
BstENI CCTNNNNNAGG 1 cut(s) 1185
BstF5I GGATG 5 cut(s) 123, 355, 1202, 1294, 1447
BstH2I RGCGCY 2 cut(s) 490, 969
BstHHI GCGC 3 cut(s) 489, 968, 1427
BstKTI GATC 4 cut(s) 249, 677, 695, 1468
BstMAI GTCTC 4 cut(s) 56, 781, 856, 1025
BstMBI GATC 4 cut(s) 246, 674, 692, 1465
BstMWI GCNNNNNNNGC 5 cut(s) 815, 965, 1122, 1331, 1366
BstNI CCWGG 4 cut(s) 159, 444, 617, 1210
BstNSI RCATGY 1 cut(s) 1373
BstSCI CCNGG 5 cut(s) 126, 157, 442, 615, 1208
BstSFI CTRYAG 1 cut(s) 1064
BstSLI GKGCMC 2 cut(s) 193, 1216
BstV1I GCAGC 3 cut(s) 802, 1050, 1346
BstX2I RGATCY 1 cut(s) 692
BstYI RGATCY 1 cut(s) 692
Bsu15I ATCGAT 1 cut(s) 1468
BsuTUI ATCGAT 1 cut(s) 1468
BtsCI GGATG 5 cut(s) 123, 355, 1202, 1294, 1447
BtsIMutI CAGTG 3 cut(s) 344, 684, 716
BveI ACCTGC 2 cut(s) 82, 1107
Cac8I GCNNGC 2 cut(s) 284, 1371
CciI TCATGA 1 cut(s) 1194
CfoI GCGC 3 cut(s) 489, 968, 1427
ClaI ATCGAT 1 cut(s) 1468
CseI GACGC 1 cut(s) 1007
Csp6I GTAC 4 cut(s) 113, 551, 826, 1231
CviAII CATG 3 cut(s) 391, 1195, 1370
CviQI GTAC 4 cut(s) 113, 551, 826, 1231
DdeI CTNAG 3 cut(s) 148, 752, 1112
DinI GGCGCC 1 cut(s) 488
DpnI GATC 4 cut(s) 248, 676, 694, 1467
DpnII GATC 4 cut(s) 246, 674, 692, 1465
DraIII CACNNNGTG 1 cut(s) 1252
DrdI GACNNNNNNGTC 1 cut(s) 1098
DseDI GACNNNNNNGTC 1 cut(s) 1098
Eam1104I CTCTTC 6 cut(s) 13, 35, 44, 63, 339, 617
EarI CTCTTC 6 cut(s) 13, 35, 44, 63, 339, 617
EciI GGCGGA 1 cut(s) 10
Ecl136II GAGCTC 2 cut(s) 384, 939
Eco24I GRGCYC 3 cut(s) 8, 386, 941
Eco53kI GAGCTC 2 cut(s) 384, 939
Eco57I CTGAAG 2 cut(s) 350, 1365
EcoICRI GAGCTC 2 cut(s) 384, 939
EcoNI CCTNNNNNAGG 1 cut(s) 1185
EcoRII CCWGG 4 cut(s) 157, 442, 615, 1208
EcoT22I ATGCAT 1 cut(s) 768
EcoT38I GRGCYC 3 cut(s) 8, 386, 941
EgeI GGCGCC 1 cut(s) 488
EheI GGCGCC 1 cut(s) 488
Esp3I CGTCTC 2 cut(s) 856, 1025
FaeI CATG 3 cut(s) 394, 1198, 1373
FaqI GGGAC 2 cut(s) 877, 1334
FatI CATG 3 cut(s) 390, 1194, 1369
FauI CCCGC 2 cut(s) 802, 1319
FblI GTMKAC 2 cut(s) 257, 1090
Fnu4HI GCNGC 3 cut(s) 816, 1064, 1335
FokI GGATG 5 cut(s) 110, 362, 1189, 1301, 1454
FriOI GRGCYC 3 cut(s) 8, 386, 941
Fsp4HI GCNGC 3 cut(s) 816, 1064, 1335
FspBI CTAG 4 cut(s) 116, 791, 1122, 1322
GlaI GCGC 3 cut(s) 488, 967, 1426
GluI GCNGC 3 cut(s) 816, 1064, 1335
GsaI CCCAGC 1 cut(s) 1047
GsuI CTGGAG 1 cut(s) 1192
HaeII RGCGCY 2 cut(s) 490, 969
HapII CCGG 1 cut(s) 128
HgaI GACGC 1 cut(s) 1007
HhaI GCGC 3 cut(s) 489, 968, 1427
Hin1I GRCGYC 2 cut(s) 487, 1018
Hin1II CATG 3 cut(s) 394, 1198, 1373
Hin6I GCGC 3 cut(s) 487, 966, 1425
HinP1I GCGC 3 cut(s) 487, 966, 1425
HinfI GANTC 8 cut(s) 270, 510, 918, 1054, 1156, 1175, 1304, 1433
HpaII CCGG 1 cut(s) 128
HphI GGTGA 3 cut(s) 63, 445, 913
Hpy166II GTNNAC 5 cut(s) 185, 239, 258, 1091, 1272
Hpy188I TCNGA 9 cut(s) 63, 142, 151, 369, 480, 1011, 1174, 1408, 1432
Hpy188III TCNNGA 3 cut(s) 1103, 1195, 1343
Hpy8I GTNNAC 5 cut(s) 185, 239, 258, 1091, 1272
HpyAV CCTTC 4 cut(s) 636, 1063, 1252, 1400
HpyCH4III ACNGT 3 cut(s) 463, 868, 1417
HpyCH4V TGCA 8 cut(s) 233, 286, 766, 818, 934, 993, 1066, 1255
HpyF10VI GCNNNNNNNGC 5 cut(s) 815, 965, 1122, 1331, 1366
HpyF3I CTNAG 3 cut(s) 148, 752, 1112
Hsp92I GRCGYC 2 cut(s) 487, 1018
Hsp92II CATG 3 cut(s) 394, 1198, 1373
HspAI GCGC 3 cut(s) 487, 966, 1425
KasI GGCGCC 1 cut(s) 486
KpnI GGTACC 1 cut(s) 554
Kzo9I GATC 4 cut(s) 246, 674, 692, 1465
LguI GCTCTTC 1 cut(s) 13
LmnI GCTCC 5 cut(s) 381, 686, 812, 1392, 1458
Lsp1109I GCAGC 3 cut(s) 802, 1050, 1346
LweI GCATC 3 cut(s) 753, 1346, 1436
MaeI CTAG 4 cut(s) 116, 791, 1122, 1322
MaeIII GTNAC 1 cut(s) 451
MalI GATC 4 cut(s) 248, 676, 694, 1467
MboI GATC 4 cut(s) 246, 674, 692, 1465
MboII GAAGA 8 cut(s) 22, 31, 50, 356, 356, 479, 482, 604
MfeI CAATTG 1 cut(s) 534
MflI RGATCY 1 cut(s) 692
MhlI GDGCHC 7 cut(s) 8, 147, 193, 386, 941, 1216, 1463
MluCI AATT 9 cut(s) 294, 534, 727, 732, 742, 798, 887, 1106, 1354
Mly113I GGCGCC 1 cut(s) 487
MlyI GAGTC 3 cut(s) 1063, 1165, 1169
MmeI TCCRAC 2 cut(s) 86, 1121
Mph1103I ATGCAT 1 cut(s) 768
MroXI GAANNNNTTC 1 cut(s) 474
MseI TTAA 4 cut(s) 215, 243, 1185, 1482
MslI CAYNNNNRTG 1 cut(s) 150
MspCI CTTAAG 2 cut(s) 242, 1481
MspI CCGG 1 cut(s) 128
MspR9I CCNGG 5 cut(s) 128, 159, 444, 617, 1210
MunI CAATTG 1 cut(s) 534
MvaI CCWGG 4 cut(s) 159, 444, 617, 1210
MwoI GCNNNNNNNGC 5 cut(s) 815, 965, 1122, 1331, 1366
NarI GGCGCC 1 cut(s) 487
NciI CCSGG 1 cut(s) 128
NdeII GATC 4 cut(s) 246, 674, 692, 1465
NlaIII CATG 3 cut(s) 394, 1198, 1373
NlaIV GGNNCC 5 cut(s) 132, 488, 531, 552, 1215
NmuCI GTSAC 1 cut(s) 451
NsiI ATGCAT 1 cut(s) 768
NspI RCATGY 1 cut(s) 1373
PaeI GCATGC 1 cut(s) 1373
PagI TCATGA 1 cut(s) 1194
PaqCI CACCTGC 1 cut(s) 82
PciSI GCTCTTC 1 cut(s) 13
PdmI GAANNNNTTC 1 cut(s) 474
PfeI GAWTC 5 cut(s) 270, 510, 918, 1304, 1433
PflFI GACNNNGTC 1 cut(s) 1095
PkrI GCNGC 3 cut(s) 817, 1065, 1336
PleI GAGTC 3 cut(s) 1062, 1164, 1169
PluTI GGCGCC 1 cut(s) 490
PpsI GAGTC 3 cut(s) 1062, 1164, 1169
PsiI TTATAA 1 cut(s) 602
Psp124BI GAGCTC 2 cut(s) 386, 941
Psp6I CCWGG 4 cut(s) 157, 442, 615, 1208
PspFI CCCAGC 1 cut(s) 1043
PspGI CCWGG 4 cut(s) 157, 442, 615, 1208
PspN4I GGNNCC 5 cut(s) 132, 488, 531, 552, 1215
PstI CTGCAG 1 cut(s) 1068
PsuI RGATCY 1 cut(s) 692
PsyI GACNNNGTC 1 cut(s) 1095
RsaI GTAC 4 cut(s) 114, 552, 827, 1232
RsaNI GTAC 4 cut(s) 113, 551, 826, 1231
RseI CAYNNNNRTG 1 cut(s) 150
SacI GAGCTC 2 cut(s) 386, 941
SapI GCTCTTC 1 cut(s) 13
SaqAI TTAA 4 cut(s) 215, 243, 1185, 1482
SatI GCNGC 3 cut(s) 816, 1064, 1335
Sau3AI GATC 4 cut(s) 246, 674, 692, 1465
ScaI AGTACT 2 cut(s) 114, 1232
SchI GAGTC 3 cut(s) 1063, 1165, 1169
ScrFI CCNGG 5 cut(s) 128, 159, 444, 617, 1210
SduI GDGCHC 7 cut(s) 8, 147, 193, 386, 941, 1216, 1463
SfaNI GCATC 3 cut(s) 753, 1346, 1436
SfcI CTRYAG 1 cut(s) 1064
SfoI GGCGCC 1 cut(s) 488
SmiMI CAYNNNNRTG 1 cut(s) 150
SmlI CTYRAG 2 cut(s) 242, 1481
SmoI CTYRAG 2 cut(s) 242, 1481
SpeI ACTAGT 1 cut(s) 790
SphI GCATGC 1 cut(s) 1373
Sse9I AATT 9 cut(s) 294, 534, 727, 732, 742, 798, 887, 1106, 1354
SsiI CCGC 4 cut(s) 21, 809, 1312, 1378
SspDI GGCGCC 1 cut(s) 486
SspI AATATT 1 cut(s) 783
SspMI CTAG 4 cut(s) 116, 791, 1122, 1322
SstI GAGCTC 2 cut(s) 386, 941
StyD4I CCNGG 5 cut(s) 126, 157, 442, 615, 1208
TaaI ACNGT 3 cut(s) 463, 868, 1417
TaqI TCGA 2 cut(s) 417, 1468
TasI AATT 9 cut(s) 294, 534, 727, 732, 742, 798, 887, 1106, 1354
TatI WGTACW 2 cut(s) 112, 1230
TfiI GAWTC 5 cut(s) 270, 510, 918, 1304, 1433
Tru1I TTAA 4 cut(s) 215, 243, 1185, 1482
Tru9I TTAA 4 cut(s) 215, 243, 1185, 1482
TscAI CASTG 3 cut(s) 344, 684, 723
TseFI GTSAC 1 cut(s) 451
TseI GCWGC 3 cut(s) 815, 1063, 1334
Tsp45I GTSAC 1 cut(s) 451
TspDTI ATGAA 6 cut(s) 299, 418, 420, 638, 695, 1425
TspGWI ACGGA 1 cut(s) 1454
TspRI CASTG 3 cut(s) 344, 684, 723
Tth111I GACNNNGTC 1 cut(s) 1095
Vha464I CTTAAG 2 cut(s) 242, 1481
XagI CCTNNNNNAGG 1 cut(s) 1185
XapI RAATTY 4 cut(s) 294, 727, 742, 1106
XceI RCATGY 1 cut(s) 1373
XmiI GTMKAC 2 cut(s) 257, 1090
XmnI GAANNNNTTC 1 cut(s) 474
XspI CTAG 4 cut(s) 116, 791, 1122, 1322
ZrmI AGTACT 2 cut(s) 114, 1232
Zsp2I ATGCAT 1 cut(s) 768
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.