RLG00000022546

NEMP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0004377
Physical Location & Seq
Forward (+)
3277092 .. 3283893
6802 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022546

Sequence Viewer

Length: 1500 bp
ATGGCTACCCTTCCTCTCTTCGTTTTCTCTCTCTTTCTTTCGACCATCTCATTCTTATGTTCCACTACCCCTACCCCTACCCCTACCGGGAAAGGTATTGGTCTTGAAAACCCAGTACTAAATGTCAACCCAGCACCGCTTTCTCTTTCTGGGCACTCGCCTACCGCTGGTTCTAAAGATGTTTTATTTTGTGAGCGGGTTCGAGTTTCTGGTATACCTAGATCAAAAATTGGGAGTTATGCAAATTCCTTTCGGATTACTCTGTCTCCATCTGTCGTAATCCCAGAGAGGTTGCATAGCAAAATTGAGGTCTGTTTTCACCGTAATGATTCGCTTGGACTCTGTCAGTGTAGAAATGATGAGTGGAAATCTCTCGAGAAAGGGCTATGGGAATCTGTCATGTCCCCTTATGAGGAGAAATATGTTGATGTCAAGTACATTGGCAATTTACTTGGGTCGTCTAGCATCACTGTTGAAGAAGTTTCTCAGCAATGGCGCCTTTGGTTTCTAGCAGTGGGATTCATTTTACTATTGCTGGCACCAAGCATCAGTAGTTGGGTTCATTTTTATTATAGCACTTCAATGGTTATAGGAGTTTTTATGGTCATTATATTCCTTCTCTTCCAGGGAATGAAATTGCTGCCATTTGGAAGAAAAAATTTCTTCTATCTTGTTACTGTATATGGATCAGTGCTTGGAGCTGGATCTTACCTCTTTCATCACTTCTCTGTCATGGTAAATTCAATTCTTGTAAATTTTGGGATTAGTGAAGACATGCACAATCTGATCTCTAAATTTTTACTGGTGGCGATTATCCTTATGGGTGCTGCACTAGGTTACTGGATTGGGAGGAAGCTTTTCATCTCAAAGGATGGAACCGTAGATGTTGTTGTAGCCCAATTTGCTATGTGGGCAATCCGCTTTTTTGGAATCACGTCCATCTCTCAGAGCACTCTCGATACTCCATTGGCTATTGGAGCGTTGGTTTCCTGCTTGGTTATCTGCAAACTTATCACTTCTGTGAAGTGGCATGGTAGAATGAATCAGTCAAATGGTGCGAGTGGGAGTCCTTTACAGCAGAAAGGAAGACATGTCACAGGAAGATACAGTCGTCCAGAGTTCCTTAGCAGGTCCAGCGCACTAGAGGCATGGAACAGTACAAGGGGTGCATCTGCTTGGTCTGATTCTCCTGTTAAAGGTGTTGTGTCGCCATCTTTTAGGACATGTGATCAACAGGAGTACTATTCAACCTTCCACAAGATGCGAAACCGAAAGAATTTCACAAAGAAAGAGTGGGATGATTTTACAATGGAATCAACACGGCATGCTCTAGCAGAATGGGCAGCATCTCCTGCATTCACCGATTGGGTTATCGAGCATGCTGACCGCATACAACTCCTTCCAAGCGATAGTTCAGATGAAACTGTGGGAAGTGAATCTGATTCTACTGATGAGAATGTCGTGGGGGGCACGGATAGGCGTGGTTTTCTCAAATGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NEMP

Protein Analysis

500

Amino Acids

55.6

Weight (kDa)

8.66

Isoelectric Point (pI)

39.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NEMP PF10225 156 - 349 1.2e-37 NEMP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1119
AccB1I GGYRCC 2 cut(s) 495, 538
AccBSI CCGCTC 1 cut(s) 196
AccI GTMKAC 1 cut(s) 214
AciI CCGC 5 cut(s) 137, 165, 196, 919, 1387
AclWI GGATC 2 cut(s) 694, 712
AcsI RAATTY 6 cut(s) 244, 658, 739, 754, 794, 1276
AcyI GRCGYC 1 cut(s) 496
AfaI GTAC 4 cut(s) 117, 437, 1159, 1241
AfiI CCNNNNNNNGG 4 cut(s) 87, 167, 412, 1196
AflIII ACRYGT 2 cut(s) 1090, 1223
AgsI TTSAA 5 cut(s) 107, 476, 582, 744, 1248
AjiI CACGTC 1 cut(s) 936
AjnI CCWGG 1 cut(s) 624
AjuI GAANNNNNNNTTGG 2 cut(s) 1396, 1428
AleI CACNNNNGTG 1 cut(s) 1019
AluBI AGCT 2 cut(s) 701, 856
AluI AGCT 2 cut(s) 701, 856
Alw21I GWGCWC 1 cut(s) 953
Alw26I GTCTC 1 cut(s) 270
AlwI GGATC 2 cut(s) 694, 712
Ama87I CYCGRG 1 cut(s) 374
ApeKI GCWGC 3 cut(s) 640, 827, 1343
ApoI RAATTY 6 cut(s) 244, 658, 739, 754, 794, 1276
ArsI GACNNNNNNTTYG 2 cut(s) 294, 326
Asp700I GAANNNNTTC 1 cut(s) 857
AspLEI GCGC 2 cut(s) 498, 1139
AspS9I GGNCC 1 cut(s) 1131
AsuC2I CCSGG 1 cut(s) 88
AsuHPI GGTGA 2 cut(s) 311, 1351
AvaI CYCGRG 1 cut(s) 374
AvaII GGWCC 1 cut(s) 1131
BaeGI GKGCMC 2 cut(s) 156, 1472
BaeI ACNNNNGTAYC 2 cut(s) 1096, 1129
BanI GGYRCC 2 cut(s) 495, 538
BbsI GAAGAC 2 cut(s) 777, 1093
Bbv12I GWGCWC 1 cut(s) 953
BbvI GCAGC 3 cut(s) 627, 814, 1355
BccI CCATC 5 cut(s) 53, 277, 866, 947, 1219
BceAI ACGGC 1 cut(s) 1337
BciT130I CCWGG 1 cut(s) 626
BclI TGATCA 1 cut(s) 1228
BcnI CCSGG 1 cut(s) 88
BcoDI GTCTC 1 cut(s) 270
BfaI CTAG 6 cut(s) 219, 462, 509, 833, 1142, 1331
BfoI RGCGCY 1 cut(s) 499
BfuAI ACCTGC 1 cut(s) 1119
BisI GCNGC 3 cut(s) 641, 828, 1344
BlsI GCNGC 3 cut(s) 642, 829, 1345
BmcAI AGTACT 2 cut(s) 117, 1241
Bme1390I CCNGG 2 cut(s) 88, 626
Bme18I GGWCC 1 cut(s) 1131
BmeT110I CYCGRG 1 cut(s) 374
BmgBI CACGTC 1 cut(s) 936
BmgT120I GGNCC 1 cut(s) 1131
BmiI GGNNCC 3 cut(s) 497, 540, 877
BmrFI CCNGG 2 cut(s) 88, 626
BmrI ACTGGG 1 cut(s) 107
BmsI GCATC 5 cut(s) 474, 555, 1178, 1251, 1355
BmuI ACTGGG 1 cut(s) 107
BpiI GAAGAC 2 cut(s) 777, 1093
Bpu10I CCTNAGC 1 cut(s) 1124
BpuMI CCSGG 1 cut(s) 88
BsaHI GRCGYC 1 cut(s) 496
BsaJI CCNNGG 1 cut(s) 625
BsaXI ACNNNNNCTCC 4 cut(s) 250, 280, 969, 999
Bsc4I CCNNNNNNNGG 4 cut(s) 87, 167, 412, 1196
Bse1I ACTGG 3 cut(s) 113, 807, 845
Bse3DI GCAATG 1 cut(s) 497
BseBI CCWGG 1 cut(s) 626
BseDI CCNNGG 1 cut(s) 625
BseGI GGATG 2 cut(s) 877, 1303
BseLI CCNNNNNNNGG 4 cut(s) 87, 167, 412, 1196
BseMI GCAATG 1 cut(s) 497
BseMII CTCAG 2 cut(s) 500, 959
BseNI ACTGG 3 cut(s) 113, 807, 845
BseRI GAGGAG 1 cut(s) 428
BseSI GKGCMC 2 cut(s) 156, 1472
BseXI GCAGC 3 cut(s) 627, 814, 1355
BseYI CCCAGC 1 cut(s) 130
BsgI GTGCAG 1 cut(s) 813
BshNI GGYRCC 2 cut(s) 495, 538
BsiHKAI GWGCWC 1 cut(s) 953
BsiHKCI CYCGRG 1 cut(s) 374
BsiSI CCGG 1 cut(s) 87
BslFI GGGAC 1 cut(s) 388
BslI CCNNNNNNNGG 4 cut(s) 87, 167, 412, 1196
BsmAI GTCTC 1 cut(s) 270
BsmFI GGGAC 1 cut(s) 388
BsmI GAATGC 1 cut(s) 1355
BsoBI CYCGRG 1 cut(s) 374
Bsp1286I GDGCHC 3 cut(s) 156, 953, 1472
Bsp143I GATC 5 cut(s) 221, 686, 704, 786, 1228
BspACI CCGC 5 cut(s) 137, 165, 196, 919, 1387
BspCNI CTCAG 2 cut(s) 499, 958
BspLI GGNNCC 3 cut(s) 497, 540, 877
BspMI ACCTGC 1 cut(s) 1119
BspPI GGATC 2 cut(s) 694, 712
BspT107I GGYRCC 2 cut(s) 495, 538
BsrBI CCGCTC 1 cut(s) 196
BsrDI GCAATG 1 cut(s) 497
BsrI ACTGG 3 cut(s) 113, 807, 845
BssECI CCNNGG 1 cut(s) 625
BssMI GATC 5 cut(s) 221, 686, 704, 786, 1228
BssNAI GTATAC 1 cut(s) 215
BssNI GRCGYC 1 cut(s) 496
Bst1107I GTATAC 1 cut(s) 215
Bst2UI CCWGG 1 cut(s) 626
Bst4CI ACNGT 7 cut(s) 323, 472, 679, 880, 1109, 1157, 1426
Bst6I CTCTTC 2 cut(s) 23, 626
BstACI GRCGYC 1 cut(s) 496
BstAPI GCANNNNNTGC 1 cut(s) 1352
BstC8I GCNNGC 3 cut(s) 537, 1326, 1380
BstDEI CTNAG 3 cut(s) 486, 945, 1124
BstENI CCTNNNNNAGG 1 cut(s) 1194
BstF5I GGATG 2 cut(s) 877, 1303
BstH2I RGCGCY 1 cut(s) 499
BstHHI GCGC 2 cut(s) 498, 1139
BstKTI GATC 5 cut(s) 224, 689, 707, 789, 1231
BstMAI GTCTC 1 cut(s) 270
BstMBI GATC 5 cut(s) 221, 686, 704, 786, 1228
BstMWI GCNNNNNNNGC 7 cut(s) 902, 911, 977, 1134, 1145, 1340, 1352
BstNI CCWGG 1 cut(s) 626
BstNSI RCATGY 5 cut(s) 778, 1094, 1227, 1328, 1382
BstSCI CCNGG 2 cut(s) 86, 624
BstSLI GKGCMC 2 cut(s) 156, 1472
BstV1I GCAGC 3 cut(s) 627, 814, 1355
BstV2I GAAGAC 2 cut(s) 777, 1093
BstX2I RGATCY 1 cut(s) 704
BstYI RGATCY 1 cut(s) 704
BstZ17I GTATAC 1 cut(s) 215
BtrI CACGTC 1 cut(s) 936
BtsCI GGATG 2 cut(s) 877, 1303
BtsI GCAGTG 1 cut(s) 519
BtsIMutI CAGTG 4 cut(s) 353, 468, 519, 696
BveI ACCTGC 1 cut(s) 1119
Cac8I GCNNGC 3 cut(s) 537, 1326, 1380
CfoI GCGC 2 cut(s) 498, 1139
Cfr13I GGNCC 1 cut(s) 1131
Csp6I GTAC 4 cut(s) 116, 436, 1158, 1240
CviAII CATG 9 cut(s) 400, 733, 775, 1031, 1091, 1149, 1224, 1325, 1379
CviJI RGCY 6 cut(s) 5, 385, 701, 856, 896, 971
CviKI_1 RGCY 6 cut(s) 5, 385, 701, 856, 896, 971
CviQI GTAC 4 cut(s) 116, 436, 1158, 1240
DdeI CTNAG 3 cut(s) 486, 945, 1124
DinI GGCGCC 1 cut(s) 497
DpnI GATC 5 cut(s) 223, 688, 706, 788, 1230
DpnII GATC 5 cut(s) 221, 686, 704, 786, 1228
Eam1104I CTCTTC 2 cut(s) 23, 626
EarI CTCTTC 2 cut(s) 23, 626
Eco47I GGWCC 1 cut(s) 1131
Eco88I CYCGRG 1 cut(s) 374
EcoNI CCTNNNNNAGG 1 cut(s) 1194
EcoRII CCWGG 1 cut(s) 624
EgeI GGCGCC 1 cut(s) 497
EheI GGCGCC 1 cut(s) 497
FaeI CATG 9 cut(s) 403, 736, 778, 1034, 1094, 1152, 1227, 1328, 1382
FaqI GGGAC 1 cut(s) 388
FatI CATG 9 cut(s) 399, 732, 774, 1030, 1090, 1148, 1223, 1324, 1378
FauI CCCGC 1 cut(s) 189
FbaI TGATCA 1 cut(s) 1228
FblI GTMKAC 1 cut(s) 214
Fnu4HI GCNGC 3 cut(s) 641, 828, 1344
FokI GGATG 2 cut(s) 884, 1310
Fsp4HI GCNGC 3 cut(s) 641, 828, 1344
FspBI CTAG 6 cut(s) 219, 462, 509, 833, 1142, 1331
GlaI GCGC 2 cut(s) 497, 1138
GluI GCNGC 3 cut(s) 641, 828, 1344
GsaI CCCAGC 1 cut(s) 134
HaeII RGCGCY 1 cut(s) 499
HapII CCGG 1 cut(s) 87
HhaI GCGC 2 cut(s) 498, 1139
Hin1I GRCGYC 1 cut(s) 496
Hin1II CATG 9 cut(s) 403, 736, 778, 1034, 1094, 1152, 1227, 1328, 1382
Hin6I GCGC 2 cut(s) 496, 1137
HinP1I GCGC 2 cut(s) 496, 1137
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HindIII AAGCTT 1 cut(s) 854
HpaII CCGG 1 cut(s) 87
HphI GGTGA 2 cut(s) 311, 1351
Hpy166II GTNNAC 2 cut(s) 127, 215
Hpy188I TCNGA 6 cut(s) 255, 786, 948, 1183, 1417, 1441
Hpy188III TCNNGA 5 cut(s) 104, 374, 376, 956, 1115
Hpy8I GTNNAC 2 cut(s) 127, 215
HpyAV CCTTC 4 cut(s) 20, 626, 1261, 1409
HpyCH4III ACNGT 7 cut(s) 323, 472, 679, 880, 1109, 1157, 1426
HpyCH4IV ACGT 1 cut(s) 935
HpyCH4V TGCA 7 cut(s) 242, 295, 778, 830, 1005, 1169, 1355
HpyF10VI GCNNNNNNNGC 7 cut(s) 902, 911, 977, 1134, 1145, 1340, 1352
HpyF3I CTNAG 3 cut(s) 486, 945, 1124
HpySE526I ACGT 1 cut(s) 935
Hsp92I GRCGYC 1 cut(s) 496
Hsp92II CATG 9 cut(s) 403, 736, 778, 1034, 1094, 1152, 1227, 1328, 1382
HspAI GCGC 2 cut(s) 496, 1137
KasI GGCGCC 1 cut(s) 495
Ksp22I TGATCA 1 cut(s) 1228
Kzo9I GATC 5 cut(s) 221, 686, 704, 786, 1228
LmnI GCTCC 2 cut(s) 698, 977
Lsp1109I GCAGC 3 cut(s) 627, 814, 1355
LweI GCATC 5 cut(s) 474, 555, 1178, 1251, 1355
MaeI CTAG 6 cut(s) 219, 462, 509, 833, 1142, 1331
MaeII ACGT 1 cut(s) 935
MaeIII GTNAC 3 cut(s) 673, 836, 1093
MalI GATC 5 cut(s) 223, 688, 706, 788, 1230
MbiI CCGCTC 1 cut(s) 196
MboI GATC 5 cut(s) 221, 686, 704, 786, 1228
MboII GAAGA 8 cut(s) 10, 488, 613, 655, 663, 782, 1098, 1113
MflI RGATCY 1 cut(s) 704
MhlI GDGCHC 3 cut(s) 156, 953, 1472
Mly113I GGCGCC 1 cut(s) 496
MlyI GAGTC 2 cut(s) 333, 1075
MnlI CCTC 7 cut(s) 24, 282, 301, 406, 722, 843, 1138
MroXI GAANNNNTTC 1 cut(s) 857
MseI TTAA 1 cut(s) 1194
MslI CAYNNNNRTG 4 cut(s) 55, 324, 581, 1019
MspA1I CMGCKG 1 cut(s) 167
MspI CCGG 1 cut(s) 87
MspR9I CCNGG 2 cut(s) 88, 626
Mva1269I GAATGC 1 cut(s) 1355
MvaI CCWGG 1 cut(s) 626
MwoI GCNNNNNNNGC 7 cut(s) 902, 911, 977, 1134, 1145, 1340, 1352
NarI GGCGCC 1 cut(s) 496
NciI CCSGG 1 cut(s) 88
NdeII GATC 5 cut(s) 221, 686, 704, 786, 1228
NlaIII CATG 9 cut(s) 403, 736, 778, 1034, 1094, 1152, 1227, 1328, 1382
NlaIV GGNNCC 3 cut(s) 497, 540, 877
NmuCI GTSAC 1 cut(s) 1093
NspI RCATGY 5 cut(s) 778, 1094, 1227, 1328, 1382
OliI CACNNNNGTG 1 cut(s) 1019
PaeI GCATGC 2 cut(s) 1328, 1382
PaeR7I CTCGAG 1 cut(s) 374
PciI ACATGT 2 cut(s) 1090, 1223
PctI GAATGC 1 cut(s) 1355
PdmI GAANNNNTTC 1 cut(s) 857
PfeI GAWTC 9 cut(s) 329, 392, 519, 930, 1042, 1184, 1313, 1436, 1442
PflFI GACNNNGTC 1 cut(s) 342
PkrI GCNGC 3 cut(s) 642, 829, 1345
PleI GAGTC 2 cut(s) 333, 1074
PluTI GGCGCC 1 cut(s) 499
PpsI GAGTC 2 cut(s) 333, 1074
PscI ACATGT 2 cut(s) 1090, 1223
Psp6I CCWGG 1 cut(s) 624
PspFI CCCAGC 1 cut(s) 130
PspGI CCWGG 1 cut(s) 624
PspN4I GGNNCC 3 cut(s) 497, 540, 877
PspPI GGNCC 1 cut(s) 1131
PsuI RGATCY 1 cut(s) 704
PsyI GACNNNGTC 1 cut(s) 342
RsaI GTAC 4 cut(s) 117, 437, 1159, 1241
RsaNI GTAC 4 cut(s) 116, 436, 1158, 1240
RseI CAYNNNNRTG 4 cut(s) 55, 324, 581, 1019
SaqAI TTAA 1 cut(s) 1194
SatI GCNGC 3 cut(s) 641, 828, 1344
Sau3AI GATC 5 cut(s) 221, 686, 704, 786, 1228
Sau96I GGNCC 1 cut(s) 1131
ScaI AGTACT 2 cut(s) 117, 1241
SchI GAGTC 2 cut(s) 333, 1075
ScrFI CCNGG 2 cut(s) 88, 626
SduI GDGCHC 3 cut(s) 156, 953, 1472
SfaNI GCATC 5 cut(s) 474, 555, 1178, 1251, 1355
SfoI GGCGCC 1 cut(s) 497
Sfr274I CTCGAG 1 cut(s) 374
SinI GGWCC 1 cut(s) 1131
SlaI CTCGAG 1 cut(s) 374
SmiMI CAYNNNNRTG 4 cut(s) 55, 324, 581, 1019
SmlI CTYRAG 1 cut(s) 374
SmoI CTYRAG 1 cut(s) 374
SphI GCATGC 2 cut(s) 1328, 1382
SsiI CCGC 5 cut(s) 137, 165, 196, 919, 1387
SspDI GGCGCC 1 cut(s) 495
SspMI CTAG 6 cut(s) 219, 462, 509, 833, 1142, 1331
StyD4I CCNGG 2 cut(s) 86, 624
TaaI ACNGT 7 cut(s) 323, 472, 679, 880, 1109, 1157, 1426
TaiI ACGT 1 cut(s) 938
TaqI TCGA 5 cut(s) 41, 202, 375, 957, 1374
TatI WGTACW 4 cut(s) 115, 435, 1157, 1239
TfiI GAWTC 9 cut(s) 329, 392, 519, 930, 1042, 1184, 1313, 1436, 1442
Tru1I TTAA 1 cut(s) 1194
Tru9I TTAA 1 cut(s) 1194
TscAI CASTG 4 cut(s) 353, 475, 519, 696
TseFI GTSAC 1 cut(s) 1093
TseI GCWGC 3 cut(s) 640, 827, 1343
Tsp45I GTSAC 1 cut(s) 1093
TspDTI ATGAA 7 cut(s) 511, 551, 647, 707, 850, 1055, 1434
TspGWI ACGGA 1 cut(s) 1487
TspRI CASTG 4 cut(s) 353, 475, 519, 696
Tth111I GACNNNGTC 1 cut(s) 342
VpaK11BI GGWCC 1 cut(s) 1131
XagI CCTNNNNNAGG 1 cut(s) 1194
XapI RAATTY 6 cut(s) 244, 658, 739, 754, 794, 1276
XceI RCATGY 5 cut(s) 778, 1094, 1227, 1328, 1382
XhoI CTCGAG 1 cut(s) 374
XmiI GTMKAC 1 cut(s) 214
XmnI GAANNNNTTC 1 cut(s) 857
XspI CTAG 6 cut(s) 219, 462, 509, 833, 1142, 1331
ZrmI AGTACT 2 cut(s) 117, 1241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.