Rh6DG078000

NEMP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0004377
Physical Location & Seq
Forward (+)
8882763 .. 8888654
5892 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG078000.1

Sequence Viewer

Length: 906 bp
ATGGCTACCCTTCCTCTCTTCGTTTTCTCTCTCTTTCTTTCGACCATCTCATTCTTATGTTCCACTACCCCTACCGGGAAAGGTATTGATCTTGAAAATCAAGTACTAAATGTCAACCCAGCACCGCTTTCTCTTTTTGGGCACTCGCCTACCGCTGGTTCTAAAGATGTATTATTTTGTGAACGGGTTCGAGTTTCTGGTGTACCTAGATCAAAAATTGGGAGTTATGCAAATTCCTTTCGGATTACTCTGTCTCCATCTGTCTTAATCCCAGAGAGGTTGCATAGCAAAATTGAGGTATGTTTTCACCGTAATGATTCGCTTGGACTCTGTCAGTGTAGAAATGATGAGTGGAAAACTCTCGAGAAAGGGCTATGGGAATCTGTCATGTCCCCTTATGAGGAGAAATATGTTGATGTCAAGTACATTGGCGATTTACTTGGGTCTTCCACCATCACTGTTGAAGAAGTTTCTCAGCAATGGCGCCTTTGGTTTCTAGCAGTGGGATTCATTTTACTATTGCTGGCACCAAGCATCAGTAGTTGGGTTCATTTTTATTATAGCACTTCAATGGCTGTCGAGTTGCCAATCTTCTTGAATGATAAAAGGTTGCTATCCACACGGTTGCTATCCACACAAGTAAAAGCACCTGTCCAAGCAAGGCAAATGAAATATGATGCTGACCCAGCGGCAGAGGCAGCTGCTGCAACTGTCCTGGGCATCCAAGTTGGGTGTGGATTGCATTCAGGAGATGAATCTAGAGCTAATGTTGCACAAGATAATGAACTTTTCGGTGTGAGTGGAGTCGGATCACTGATGAAACGCGAATCTGATTTCTTCTTGTTCTTTTTTGGATACATTTCATTGCGAGCTTCTCAGGGTCTTGGGGTCCGTTGGAGGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NEMP

Protein Analysis

301

Amino Acids

33.34

Weight (kDa)

6.95

Isoelectric Point (pI)

39.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NEMP PF10225 152 - 193 4.1e-09 NEMP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 483, 526
AccII CGCG 1 cut(s) 825
AciI CCGC 3 cut(s) 125, 153, 689
AclWI GGATC 1 cut(s) 817
AcsI RAATTY 1 cut(s) 232
AcyI GRCGYC 1 cut(s) 484
AfaI GTAC 3 cut(s) 105, 204, 425
AfiI CCNNNNNNNGG 4 cut(s) 75, 155, 400, 898
AgsI TTSAA 4 cut(s) 95, 464, 570, 598
AjnI CCWGG 1 cut(s) 714
AluBI AGCT 3 cut(s) 701, 764, 872
AluI AGCT 3 cut(s) 701, 764, 872
Alw26I GTCTC 1 cut(s) 258
AlwI GGATC 1 cut(s) 817
AlwNI CAGNNNCTG 1 cut(s) 704
Ama87I CYCGRG 1 cut(s) 362
ApeKI GCWGC 3 cut(s) 698, 701, 704
ApoI RAATTY 1 cut(s) 232
Asp700I GAANNNNTTC 1 cut(s) 186
AspLEI GCGC 1 cut(s) 486
AspS9I GGNCC 1 cut(s) 889
AsuC2I CCSGG 1 cut(s) 76
AsuHPI GGTGA 1 cut(s) 299
AvaI CYCGRG 1 cut(s) 362
AvaII GGWCC 1 cut(s) 889
BaeGI GKGCMC 1 cut(s) 144
BanI GGYRCC 2 cut(s) 483, 526
BbsI GAAGAC 1 cut(s) 438
BbvI GCAGC 3 cut(s) 688, 691, 710
BccI CCATC 3 cut(s) 53, 265, 461
BciT130I CCWGG 1 cut(s) 716
BciVI GTATCC 1 cut(s) 848
BcnI CCSGG 1 cut(s) 76
BcoDI GTCTC 1 cut(s) 258
BfaI CTAG 3 cut(s) 207, 497, 759
BfoI RGCGCY 1 cut(s) 487
BfuI GTATCC 1 cut(s) 848
BisI GCNGC 4 cut(s) 690, 699, 702, 705
BlsI GCNGC 4 cut(s) 691, 700, 703, 706
BmcAI AGTACT 1 cut(s) 105
Bme1390I CCNGG 2 cut(s) 76, 716
Bme18I GGWCC 1 cut(s) 889
BmeT110I CYCGRG 1 cut(s) 362
BmgT120I GGNCC 1 cut(s) 889
BmiI GGNNCC 3 cut(s) 485, 528, 890
BmrFI CCNGG 2 cut(s) 76, 716
BmsI GCATC 3 cut(s) 543, 667, 729
BpiI GAAGAC 1 cut(s) 438
BpuMI CCSGG 1 cut(s) 76
BsaHI GRCGYC 1 cut(s) 484
BsaJI CCNNGG 1 cut(s) 715
BsaXI ACNNNNNCTCC 2 cut(s) 238, 268
Bsc4I CCNNNNNNNGG 4 cut(s) 75, 155, 400, 898
Bse3DI GCAATG 2 cut(s) 485, 863
BseBI CCWGG 1 cut(s) 716
BseDI CCNNGG 1 cut(s) 715
BseGI GGATG 1 cut(s) 720
BseLI CCNNNNNNNGG 4 cut(s) 75, 155, 400, 898
BseMI GCAATG 2 cut(s) 485, 863
BseMII CTCAG 2 cut(s) 488, 890
BseRI GAGGAG 1 cut(s) 416
BseSI GKGCMC 1 cut(s) 144
BseXI GCAGC 3 cut(s) 688, 691, 710
BseYI CCCAGC 2 cut(s) 118, 685
Bsh1236I CGCG 1 cut(s) 825
BshNI GGYRCC 2 cut(s) 483, 526
BsiHKCI CYCGRG 1 cut(s) 362
BsiSI CCGG 1 cut(s) 75
BslFI GGGAC 1 cut(s) 376
BslI CCNNNNNNNGG 4 cut(s) 75, 155, 400, 898
BsmAI GTCTC 1 cut(s) 258
BsmFI GGGAC 1 cut(s) 376
BsmI GAATGC 1 cut(s) 742
BsoBI CYCGRG 1 cut(s) 362
Bsp1286I GDGCHC 1 cut(s) 144
Bsp143I GATC 3 cut(s) 88, 209, 809
BspACI CCGC 3 cut(s) 125, 153, 689
BspCNI CTCAG 2 cut(s) 487, 889
BspFNI CGCG 1 cut(s) 825
BspLI GGNNCC 3 cut(s) 485, 528, 890
BspPI GGATC 1 cut(s) 817
BspT107I GGYRCC 2 cut(s) 483, 526
BsrDI GCAATG 2 cut(s) 485, 863
BssECI CCNNGG 1 cut(s) 715
BssMI GATC 3 cut(s) 88, 209, 809
BssNI GRCGYC 1 cut(s) 484
Bst2UI CCWGG 1 cut(s) 716
Bst4CI ACNGT 4 cut(s) 311, 460, 624, 712
Bst6I CTCTTC 1 cut(s) 23
BstACI GRCGYC 1 cut(s) 484
BstAPI GCANNNNNTGC 1 cut(s) 704
BstC8I GCNNGC 2 cut(s) 525, 870
BstDEI CTNAG 2 cut(s) 474, 876
BstF5I GGATG 1 cut(s) 720
BstFNI CGCG 1 cut(s) 825
BstH2I RGCGCY 1 cut(s) 487
BstHHI GCGC 1 cut(s) 486
BstKTI GATC 3 cut(s) 91, 212, 812
BstMAI GTCTC 1 cut(s) 258
BstMBI GATC 3 cut(s) 88, 209, 809
BstMWI GCNNNNNNNGC 5 cut(s) 686, 695, 698, 704, 770
BstNI CCWGG 1 cut(s) 716
BstSCI CCNGG 2 cut(s) 74, 714
BstSLI GKGCMC 1 cut(s) 144
BstUI CGCG 1 cut(s) 825
BstV1I GCAGC 3 cut(s) 688, 691, 710
BstV2I GAAGAC 1 cut(s) 438
BsuI GTATCC 1 cut(s) 848
BtsCI GGATG 1 cut(s) 720
BtsI GCAGTG 1 cut(s) 507
BtsIMutI CAGTG 4 cut(s) 341, 456, 507, 812
Cac8I GCNNGC 2 cut(s) 525, 870
CaiI CAGNNNCTG 1 cut(s) 704
CfoI GCGC 1 cut(s) 486
Cfr13I GGNCC 1 cut(s) 889
Csp6I GTAC 3 cut(s) 104, 203, 424
CviAII CATG 1 cut(s) 388
CviJI RGCY 6 cut(s) 5, 373, 575, 701, 764, 872
CviKI_1 RGCY 6 cut(s) 5, 373, 575, 701, 764, 872
CviQI GTAC 3 cut(s) 104, 203, 424
DdeI CTNAG 2 cut(s) 474, 876
DinI GGCGCC 1 cut(s) 485
DpnI GATC 3 cut(s) 90, 211, 811
DpnII GATC 3 cut(s) 88, 209, 809
Eam1104I CTCTTC 1 cut(s) 23
EarI CTCTTC 1 cut(s) 23
Eco47I GGWCC 1 cut(s) 889
Eco88I CYCGRG 1 cut(s) 362
EcoRII CCWGG 1 cut(s) 714
EgeI GGCGCC 1 cut(s) 485
EheI GGCGCC 1 cut(s) 485
FaeI CATG 1 cut(s) 391
FaqI GGGAC 1 cut(s) 376
FatI CATG 1 cut(s) 387
Fnu4HI GCNGC 4 cut(s) 690, 699, 702, 705
FokI GGATG 1 cut(s) 707
Fsp4HI GCNGC 4 cut(s) 690, 699, 702, 705
FspBI CTAG 3 cut(s) 207, 497, 759
GlaI GCGC 1 cut(s) 485
GluI GCNGC 4 cut(s) 690, 699, 702, 705
GsaI CCCAGC 2 cut(s) 122, 689
HaeII RGCGCY 1 cut(s) 487
HapII CCGG 1 cut(s) 75
HhaI GCGC 1 cut(s) 486
Hin1I GRCGYC 1 cut(s) 484
Hin1II CATG 1 cut(s) 391
Hin6I GCGC 1 cut(s) 484
HinP1I GCGC 1 cut(s) 484
HincII GTYRAC 1 cut(s) 115
HindII GTYRAC 1 cut(s) 115
HinfI GANTC 7 cut(s) 317, 327, 380, 507, 755, 804, 827
HpaII CCGG 1 cut(s) 75
HphI GGTGA 1 cut(s) 299
Hpy166II GTNNAC 3 cut(s) 115, 182, 203
Hpy188I TCNGA 3 cut(s) 243, 809, 832
Hpy188III TCNNGA 6 cut(s) 92, 362, 364, 595, 747, 759
Hpy8I GTNNAC 3 cut(s) 115, 182, 203
HpyAV CCTTC 1 cut(s) 20
HpyCH4III ACNGT 4 cut(s) 311, 460, 624, 712
HpyCH4V TGCA 5 cut(s) 230, 283, 707, 742, 773
HpyF10VI GCNNNNNNNGC 5 cut(s) 686, 695, 698, 704, 770
HpyF3I CTNAG 2 cut(s) 474, 876
Hsp92I GRCGYC 1 cut(s) 484
Hsp92II CATG 1 cut(s) 391
HspAI GCGC 1 cut(s) 484
KasI GGCGCC 1 cut(s) 483
Kzo9I GATC 3 cut(s) 88, 209, 809
Lsp1109I GCAGC 3 cut(s) 688, 691, 710
LweI GCATC 3 cut(s) 543, 667, 729
MaeI CTAG 3 cut(s) 207, 497, 759
MalI GATC 3 cut(s) 90, 211, 811
MboI GATC 3 cut(s) 88, 209, 809
MboII GAAGA 5 cut(s) 10, 438, 476, 583, 829
MhlI GDGCHC 1 cut(s) 144
MluCI AATT 3 cut(s) 216, 232, 291
Mly113I GGCGCC 1 cut(s) 484
MlyI GAGTC 2 cut(s) 321, 813
MmeI TCCRAC 2 cut(s) 787, 875
MnlI CCTC 6 cut(s) 24, 270, 289, 394, 688, 891
MroXI GAANNNNTTC 1 cut(s) 186
MseI TTAA 1 cut(s) 266
MslI CAYNNNNRTG 3 cut(s) 55, 312, 569
MspA1I CMGCKG 3 cut(s) 155, 689, 701
MspI CCGG 1 cut(s) 75
MspR9I CCNGG 2 cut(s) 76, 716
Mva1269I GAATGC 1 cut(s) 742
MvaI CCWGG 1 cut(s) 716
MvnI CGCG 1 cut(s) 825
MwoI GCNNNNNNNGC 5 cut(s) 686, 695, 698, 704, 770
NarI GGCGCC 1 cut(s) 484
NciI CCSGG 1 cut(s) 76
NdeII GATC 3 cut(s) 88, 209, 809
NlaIII CATG 1 cut(s) 391
NlaIV GGNNCC 3 cut(s) 485, 528, 890
PaeR7I CTCGAG 1 cut(s) 362
PctI GAATGC 1 cut(s) 742
PdmI GAANNNNTTC 1 cut(s) 186
PfeI GAWTC 5 cut(s) 317, 380, 507, 755, 827
PflFI GACNNNGTC 1 cut(s) 330
PkrI GCNGC 4 cut(s) 691, 700, 703, 706
PleI GAGTC 2 cut(s) 321, 812
PluTI GGCGCC 1 cut(s) 487
PpsI GAGTC 2 cut(s) 321, 812
Psp6I CCWGG 1 cut(s) 714
PspFI CCCAGC 2 cut(s) 118, 685
PspGI CCWGG 1 cut(s) 714
PspN4I GGNNCC 3 cut(s) 485, 528, 890
PspPI GGNCC 1 cut(s) 889
PstNI CAGNNNCTG 1 cut(s) 704
PsyI GACNNNGTC 1 cut(s) 330
PvuII CAGCTG 1 cut(s) 701
RsaI GTAC 3 cut(s) 105, 204, 425
RsaNI GTAC 3 cut(s) 104, 203, 424
RseI CAYNNNNRTG 3 cut(s) 55, 312, 569
SaqAI TTAA 1 cut(s) 266
SatI GCNGC 4 cut(s) 690, 699, 702, 705
Sau3AI GATC 3 cut(s) 88, 209, 809
Sau96I GGNCC 1 cut(s) 889
ScaI AGTACT 1 cut(s) 105
SchI GAGTC 2 cut(s) 321, 813
ScrFI CCNGG 2 cut(s) 76, 716
SduI GDGCHC 1 cut(s) 144
SetI ASST 9 cut(s) 85, 208, 281, 300, 611, 652, 703, 766, 874
SfaNI GCATC 3 cut(s) 543, 667, 729
SfoI GGCGCC 1 cut(s) 485
Sfr274I CTCGAG 1 cut(s) 362
SinI GGWCC 1 cut(s) 889
SlaI CTCGAG 1 cut(s) 362
SmiMI CAYNNNNRTG 3 cut(s) 55, 312, 569
SmlI CTYRAG 1 cut(s) 362
SmoI CTYRAG 1 cut(s) 362
Sse9I AATT 3 cut(s) 216, 232, 291
SsiI CCGC 3 cut(s) 125, 153, 689
SspDI GGCGCC 1 cut(s) 483
SspMI CTAG 3 cut(s) 207, 497, 759
StyD4I CCNGG 2 cut(s) 74, 714
TaaI ACNGT 4 cut(s) 311, 460, 624, 712
TaqI TCGA 4 cut(s) 41, 190, 363, 579
TasI AATT 3 cut(s) 216, 232, 291
TatI WGTACW 2 cut(s) 103, 423
TauI GCSGC 1 cut(s) 692
TfiI GAWTC 5 cut(s) 317, 380, 507, 755, 827
Tru1I TTAA 1 cut(s) 266
Tru9I TTAA 1 cut(s) 266
TscAI CASTG 4 cut(s) 341, 463, 507, 819
TseI GCWGC 3 cut(s) 698, 701, 704
TspDTI ATGAA 7 cut(s) 499, 539, 683, 768, 798, 833, 852
TspGWI ACGGA 1 cut(s) 881
TspRI CASTG 4 cut(s) 341, 463, 507, 819
Tth111I GACNNNGTC 1 cut(s) 330
VpaK11BI GGWCC 1 cut(s) 889
XapI RAATTY 1 cut(s) 232
XbaI TCTAGA 1 cut(s) 758
XcmI CCANNNNNNNNNTGG 1 cut(s) 731
XhoI CTCGAG 1 cut(s) 362
XmnI GAANNNNTTC 1 cut(s) 186
XspI CTAG 3 cut(s) 207, 497, 759
ZrmI AGTACT 1 cut(s) 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.