Rroxscaffold_2G00132890

NEMP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0004377
Physical Location & Seq
Reverse (-)
69924675 .. 69929400
4726 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00132890.1

Sequence Viewer

Length: 771 bp
ATGGCTACCCTTCCTCTCTTCGTTTTCTCTCTCTTTCATTCGACCATCTCATTCTTATGTTCCACTACCCCTACCGGGAAAGGTATTGATCTTGAAAATCCAATACTAAATGTCAACCCAGCACCGCTTTCTCTTTCTGGACACTCGCCTACCGCTGGTTCTAAAGATGTTTTATTTTGTGAACGGGTTCGAGTTTCTGGTGTACCTAGATCAAAAATTGGGAGTTATGCAAATTCCTTTCGGAGTACTCTGTCTCCATCTGTCTTAATCCCAGAGAGGTTGCATAGCAAAATTGAGGTCTGTTTTCACTGTAATGATTCTCTTGGACTCTGTCAGTGTAGAAATGATGAGTGGAAAACTCTCGAGAAAGGGCTATGGGAATCTGTCATGTCCCCTTATGAGGAGAAATATGTTGATGTCAAGTACATTGGCGATTTAATTGGGTCTTCCACCATCACTGTTGAAGAAGTTTCTCAGCAATGGCTCCTTTGGTTTCTAGCAGTGGGATTCATTTTACTATTGTTGGCACCAAGCATCAGTAGTTGGGTTCATTTTTATTATAGCACTTCAATGGTTATAGGAGTTTTTATGGTCATTATATTCCTTCTCATCCAGATATCTCCTAGGTCAAGAAGCTATGCAACTGTGGCTGAACTTAATAGCTGGAACATGTACTCAAGCTTAAATAAGAGCCATAGAGCACTTCCTTCTACTTCTAGAAATGATATGACGATGCGTCTGCAATGGCTGTCGAGTTGCAAATCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NEMP

Protein Analysis

256

Amino Acids

28.68

Weight (kDa)

8.15

Isoelectric Point (pI)

52.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NEMP PF10225 152 - 208 8.7e-15 NEMP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 526
AciI CCGC 2 cut(s) 125, 153
AcsI RAATTY 1 cut(s) 232
AfaI GTAC 4 cut(s) 204, 247, 425, 674
AfiI CCNNNNNNNGG 3 cut(s) 75, 155, 400
AflIII ACRYGT 1 cut(s) 669
AgsI TTSAA 3 cut(s) 95, 464, 570
AhdI GACNNNNNGTC 1 cut(s) 735
AluBI AGCT 3 cut(s) 636, 663, 681
AluI AGCT 3 cut(s) 636, 663, 681
Alw21I GWGCWC 1 cut(s) 703
Alw26I GTCTC 1 cut(s) 258
Ama87I CYCGRG 1 cut(s) 362
ApoI RAATTY 1 cut(s) 232
ArsI GACNNNNNNTTYG 2 cut(s) 282, 314
Asp700I GAANNNNTTC 1 cut(s) 186
AspA2I CCTAGG 1 cut(s) 623
AsuC2I CCSGG 1 cut(s) 76
AvaI CYCGRG 1 cut(s) 362
AvrII CCTAGG 1 cut(s) 623
BanI GGYRCC 1 cut(s) 526
BbsI GAAGAC 1 cut(s) 438
Bbv12I GWGCWC 1 cut(s) 703
BccI CCATC 3 cut(s) 53, 265, 461
BcgI CGANNNNNNTGC 2 cut(s) 721, 755
BcnI CCSGG 1 cut(s) 76
BcoDI GTCTC 1 cut(s) 258
BfaI CTAG 4 cut(s) 207, 497, 624, 717
BlnI CCTAGG 1 cut(s) 623
BmcAI AGTACT 1 cut(s) 247
Bme1390I CCNGG 1 cut(s) 76
BmeRI GACNNNNNGTC 1 cut(s) 735
BmeT110I CYCGRG 1 cut(s) 362
BmiI GGNNCC 2 cut(s) 485, 528
BmrFI CCNGG 1 cut(s) 76
BmsI GCATC 2 cut(s) 543, 723
BpiI GAAGAC 1 cut(s) 438
BpuEI CTTGAG 1 cut(s) 661
BpuMI CCSGG 1 cut(s) 76
BsaJI CCNNGG 1 cut(s) 623
BsaXI ACNNNNNCTCC 2 cut(s) 238, 268
Bsc4I CCNNNNNNNGG 3 cut(s) 75, 155, 400
Bse3DI GCAATG 2 cut(s) 485, 749
BseDI CCNNGG 1 cut(s) 623
BseGI GGATG 1 cut(s) 609
BseLI CCNNNNNNNGG 3 cut(s) 75, 155, 400
BseMI GCAATG 2 cut(s) 485, 749
BseMII CTCAG 1 cut(s) 488
BseRI GAGGAG 1 cut(s) 416
BseYI CCCAGC 1 cut(s) 118
BshNI GGYRCC 1 cut(s) 526
BsiHKAI GWGCWC 1 cut(s) 703
BsiHKCI CYCGRG 1 cut(s) 362
BsiSI CCGG 1 cut(s) 75
BslFI GGGAC 1 cut(s) 376
BslI CCNNNNNNNGG 3 cut(s) 75, 155, 400
BsmAI GTCTC 1 cut(s) 258
BsmFI GGGAC 1 cut(s) 376
BsoBI CYCGRG 1 cut(s) 362
Bsp1286I GDGCHC 1 cut(s) 703
Bsp143I GATC 2 cut(s) 88, 209
BspACI CCGC 2 cut(s) 125, 153
BspCNI CTCAG 1 cut(s) 487
BspLI GGNNCC 2 cut(s) 485, 528
BspT107I GGYRCC 1 cut(s) 526
BsrDI GCAATG 2 cut(s) 485, 749
BssECI CCNNGG 1 cut(s) 623
BssMI GATC 2 cut(s) 88, 209
BssT1I CCWWGG 1 cut(s) 623
Bst4CI ACNGT 3 cut(s) 311, 460, 646
Bst6I CTCTTC 1 cut(s) 23
BstDEI CTNAG 1 cut(s) 474
BstF5I GGATG 1 cut(s) 609
BstKTI GATC 2 cut(s) 91, 212
BstMAI GTCTC 1 cut(s) 258
BstMBI GATC 2 cut(s) 88, 209
BstMWI GCNNNNNNNGC 1 cut(s) 647
BstNSI RCATGY 1 cut(s) 673
BstSCI CCNGG 1 cut(s) 74
BstV2I GAAGAC 1 cut(s) 438
BtsCI GGATG 1 cut(s) 609
BtsI GCAGTG 1 cut(s) 507
BtsIMutI CAGTG 4 cut(s) 307, 341, 456, 507
CseI GACGC 1 cut(s) 725
Csp6I GTAC 4 cut(s) 203, 246, 424, 673
CviAII CATG 2 cut(s) 388, 670
CviJI RGCY 9 cut(s) 5, 373, 484, 636, 650, 663, 681, 693, 748
CviKI_1 RGCY 9 cut(s) 5, 373, 484, 636, 650, 663, 681, 693, 748
CviQI GTAC 4 cut(s) 203, 246, 424, 673
DdeI CTNAG 1 cut(s) 474
DpnI GATC 2 cut(s) 90, 211
DpnII GATC 2 cut(s) 88, 209
DriI GACNNNNNGTC 1 cut(s) 735
Eam1104I CTCTTC 1 cut(s) 23
Eam1105I GACNNNNNGTC 1 cut(s) 735
EarI CTCTTC 1 cut(s) 23
Eco130I CCWWGG 1 cut(s) 623
Eco32I GATATC 1 cut(s) 618
Eco88I CYCGRG 1 cut(s) 362
EcoRV GATATC 1 cut(s) 618
EcoT14I CCWWGG 1 cut(s) 623
ErhI CCWWGG 1 cut(s) 623
FaeI CATG 2 cut(s) 391, 673
FaqI GGGAC 1 cut(s) 376
FatI CATG 2 cut(s) 387, 669
FokI GGATG 1 cut(s) 596
FspBI CTAG 4 cut(s) 207, 497, 624, 717
GsaI CCCAGC 1 cut(s) 122
HapII CCGG 1 cut(s) 75
HgaI GACGC 1 cut(s) 725
Hin1II CATG 2 cut(s) 391, 673
HincII GTYRAC 1 cut(s) 115
HindII GTYRAC 1 cut(s) 115
HindIII AAGCTT 1 cut(s) 679
HinfI GANTC 4 cut(s) 317, 327, 380, 507
HpaII CCGG 1 cut(s) 75
Hpy166II GTNNAC 3 cut(s) 115, 182, 203
Hpy188I TCNGA 1 cut(s) 243
Hpy188III TCNNGA 8 cut(s) 92, 138, 362, 364, 613, 630, 717, 768
Hpy8I GTNNAC 3 cut(s) 115, 182, 203
HpyAV CCTTC 3 cut(s) 20, 614, 717
HpyCH4III ACNGT 3 cut(s) 311, 460, 646
HpyCH4V TGCA 5 cut(s) 230, 283, 641, 742, 759
HpyF10VI GCNNNNNNNGC 1 cut(s) 647
HpyF3I CTNAG 1 cut(s) 474
Hsp92II CATG 2 cut(s) 391, 673
Kzo9I GATC 2 cut(s) 88, 209
LmnI GCTCC 1 cut(s) 489
LpnPI CCDG 8 cut(s) 88, 123, 132, 141, 183, 285, 626, 649
LweI GCATC 2 cut(s) 543, 723
MaeI CTAG 4 cut(s) 207, 497, 624, 717
MalI GATC 2 cut(s) 90, 211
MboI GATC 2 cut(s) 88, 209
MboII GAAGA 4 cut(s) 10, 438, 476, 756
MhlI GDGCHC 1 cut(s) 703
MluCI AATT 4 cut(s) 216, 232, 291, 438
MlyI GAGTC 1 cut(s) 321
MnlI CCTC 4 cut(s) 24, 270, 289, 394
MroXI GAANNNNTTC 1 cut(s) 186
MseI TTAA 4 cut(s) 266, 437, 657, 683
MslI CAYNNNNRTG 3 cut(s) 55, 312, 569
MspA1I CMGCKG 1 cut(s) 155
MspI CCGG 1 cut(s) 75
MspR9I CCNGG 1 cut(s) 76
MwoI GCNNNNNNNGC 1 cut(s) 647
NciI CCSGG 1 cut(s) 76
NdeII GATC 2 cut(s) 88, 209
NlaIII CATG 2 cut(s) 391, 673
NlaIV GGNNCC 2 cut(s) 485, 528
NspI RCATGY 1 cut(s) 673
PaeR7I CTCGAG 1 cut(s) 362
PciI ACATGT 1 cut(s) 669
PdmI GAANNNNTTC 1 cut(s) 186
PfeI GAWTC 3 cut(s) 317, 380, 507
PflFI GACNNNGTC 1 cut(s) 330
PleI GAGTC 1 cut(s) 321
PpsI GAGTC 1 cut(s) 321
PscI ACATGT 1 cut(s) 669
PspFI CCCAGC 1 cut(s) 118
PspN4I GGNNCC 2 cut(s) 485, 528
PsyI GACNNNGTC 1 cut(s) 330
RsaI GTAC 4 cut(s) 204, 247, 425, 674
RsaNI GTAC 4 cut(s) 203, 246, 424, 673
RseI CAYNNNNRTG 3 cut(s) 55, 312, 569
SaqAI TTAA 4 cut(s) 266, 437, 657, 683
Sau3AI GATC 2 cut(s) 88, 209
ScaI AGTACT 1 cut(s) 247
SchI GAGTC 1 cut(s) 321
ScrFI CCNGG 1 cut(s) 76
SduI GDGCHC 1 cut(s) 703
SetI ASST 8 cut(s) 85, 208, 281, 300, 629, 638, 665, 683
SfaNI GCATC 2 cut(s) 543, 723
Sfr274I CTCGAG 1 cut(s) 362
SlaI CTCGAG 1 cut(s) 362
SmiMI CAYNNNNRTG 3 cut(s) 55, 312, 569
SmlI CTYRAG 2 cut(s) 362, 676
SmoI CTYRAG 2 cut(s) 362, 676
Sse9I AATT 4 cut(s) 216, 232, 291, 438
SsiI CCGC 2 cut(s) 125, 153
SspMI CTAG 4 cut(s) 207, 497, 624, 717
StyD4I CCNGG 1 cut(s) 74
StyI CCWWGG 1 cut(s) 623
TaaI ACNGT 3 cut(s) 311, 460, 646
TaqI TCGA 4 cut(s) 41, 190, 363, 752
TasI AATT 4 cut(s) 216, 232, 291, 438
TatI WGTACW 3 cut(s) 245, 423, 672
TfiI GAWTC 3 cut(s) 317, 380, 507
Tru1I TTAA 4 cut(s) 266, 437, 657, 683
Tru9I TTAA 4 cut(s) 266, 437, 657, 683
TscAI CASTG 4 cut(s) 314, 341, 463, 507
TspDTI ATGAA 3 cut(s) 26, 499, 539
TspRI CASTG 4 cut(s) 314, 341, 463, 507
Tth111I GACNNNGTC 1 cut(s) 330
XapI RAATTY 1 cut(s) 232
XbaI TCTAGA 1 cut(s) 716
XceI RCATGY 1 cut(s) 673
XhoI CTCGAG 1 cut(s) 362
XmaJI CCTAGG 1 cut(s) 623
XmnI GAANNNNTTC 1 cut(s) 186
XspI CTAG 4 cut(s) 207, 497, 624, 717
ZrmI AGTACT 1 cut(s) 247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.