MD09G1231100.v1.1

NEMP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0004377
Physical Location & Seq
Reverse (-)
28619210 .. 28626322
7113 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1231100.v1.1.491

Sequence Viewer

Length: 1935 bp
ATGGGCTCTTCTCTCTCCTCCGCCATTCTCATTCTTCTCTTTGCTTTCTGTCTCTCTGTTCCGACCCTCTCACTTGCCACCGAGCTCGAGGGTGTTGATATTGAAAACCCAGTACTAGAAGTCATCCCAGCACCTCTTTCTGAGCACTCAGCTGTGCCTGGTTCTAAAGATGTTTTATTTTGTGAACGGGCACGAGTTTCTGGTATATCCAGGTTAAAACTTGGGAGTTATGCAAGTTCACTTAAGATCACCTTGTTTACATCTCTTGTAATCCCGGAGAGGTTGCAGAGCAAAATTCAAGTCTGTTTTCATAGGAATAATACGCTTGGATTATGTCAGTGTGTAGAGGATGATTGGAAAAATCTTCAGAAAGGGTTATGGAGCTCTGTCATGTCGCCTTATGATGAAAGATATGTTGATGTCAAGTTCATTGGCAAAATACCTGACTCTGTCACCATAACTGTTGAAGAAGATTTTCAGAGATGGCGCCTTGTGTGTCTTGCACTGGGATTTACTTTACTATTGTTGGCACCAATTGTCAGCAATTGGGTACCTTTTTATTATAGCACTTCAATGGTTATAGGAGTTTTTATGGTCATGATAATCATTCTCTTCCAGGGAATGAAGCTGTTGCCAACTGGAAGGAAAAATGTCCTCTATCTTACCATATATGGATCAGTGCTTGGAGCAGGATCTTTCCTTGTTCCTCAGTTTTCACTGATGGTAAATTCTATTCGTTTGAGTTTTGGGCTAAGTGAAGAGATGCATAACCCAGTCTCTATATTTTTACTAGTGGGAATTATCCCTGCGGGAGCTGCATTAGGGTACTGGATTGTGAGGGAGTTTGTTGTCTCAAAAGATGGGACAGTAGATGTTGGTATAGCACAATTTGTCAAGTGGGCTATGCGTATCATTGGAACAACCTCCATCTTTCAGAGCACTCTCGATACTCCTTTAGCAATGGGCGCTTTGGTTTCTTACTGGATTATCATCAAGTTGATTACTTCTTTGAAATGGCATCTCAAATTTCATCAGTCGGATGCTTGGAGTGGGAGTCCTTGGCTGCAGAAGGGAAAACAGGTCAAGGGTAGACAAAGTCGTCTAGGATTTCTTAGCAGGAGTCTATCTGCTTGGTCTGACTCTCCTGTTAAAGATTCCAAGTACACACAAGAGTTGCAGTTCCAAGAGGCTCTAATGTCCTCTTCTGCAATCACCTCCCAATCGACACACAATGGCGGTTCCTCTTCCTCTTCATCATCGGCAGCAATCCAAGCAACCCAAGCAATCCAATCCAACCCACCACAACCATCAACACAAATCCTTTGTGAGATTTGTGTTGAAATGATAGAGGTTGACGAGATGTTTCAAAATGAGGGCTGCGTTCACTCCTTCTGCTCTGACTGCATAACCAAGCATGTGGCATCCAAAATCCAAGCAAACATTCACATAGTTTCGTGCCCTGGCTTGGACTGCAGGGCTGTGCTGGAACTTGATGCTTGTATGCCAATACTTCCCAAAGAAGTAATAGAAAGGTGGAATGATGCCCTTTGTGAAGCAATGGTTTTAGGGGCACAAAGACTCTACTGTCCTTTCAGTGACTGCTCAACGGTTTTGATGATTGACAACGAAGGTGAGGAAGCTGTAAGAGTGTCCGAGTGTCCCATTTGCCATAGACTGTTCTGTGCACGATGTCAAGTCCCTTGGCATCCAGGAGTTGATTGTGAGGAGTATCGGAGGCTTAACGAGGACGAACGAGGGAGGGCGGATCTTATGGTTAAGGAACTTGCAAAGCAAAAGAAGTGGAAGCGGTGCCCTAGGTGCAAATATTATGTGGAGAAGACTCAAGGTTGTTTGCATATTACTTGCAGGTGCCAATACCAGTTCTGCTATGGATGTGGAGCAGAATGGACAAGTACTCATGGTGGTTGCCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR NEMP

Protein Analysis

645

Amino Acids

71.69

Weight (kDa)

6.67

Isoelectric Point (pI)

45.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NEMP PF10225 152 - 341 3e-45 NEMP family
IBR PF01485 511 - 574 8.5e-11 IBR domain, a half RING-finger domain
IBR PF01485 592 - 643 2.3e-09 IBR domain, a half RING-finger domain
Rcat_RNF216 PF26200 597 - 640 9.8e-06 RNF216 Rcat domain
IBR_1 PF22191 601 - 639 9.4e-08 IBR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1857
AasI GACNNNNNNGTC 2 cut(s) 1098, 1584
Acc36I ACCTGC 1 cut(s) 1857
Acc65I GGTACC 1 cut(s) 550
AccB1I GGYRCC 5 cut(s) 486, 529, 550, 1809, 1869
AccI GTMKAC 1 cut(s) 1090
AciI CCGC 5 cut(s) 21, 809, 1236, 1763, 1807
AclWI GGATC 3 cut(s) 682, 700, 1773
AcsI RAATTY 3 cut(s) 294, 727, 1025
AcuI CTGAAG 1 cut(s) 350
AcyI GRCGYC 1 cut(s) 487
AfaI GTAC 5 cut(s) 114, 552, 827, 1163, 1915
AfiI CCNNNNNNNGG 1 cut(s) 1465
AflII CTTAAG 1 cut(s) 242
AgsI TTSAA 7 cut(s) 104, 299, 467, 573, 1012, 1340, 1367
AhlI ACTAGT 1 cut(s) 790
AjnI CCWGG 5 cut(s) 157, 209, 615, 1459, 1708
AjuI GAANNNNNNNTTGG 2 cut(s) 1425, 1457
AluBI AGCT 6 cut(s) 85, 152, 384, 628, 815, 1640
AluI AGCT 6 cut(s) 85, 152, 384, 628, 815, 1640
Alw21I GWGCWC 5 cut(s) 87, 147, 386, 941, 1687
Alw26I GTCTC 3 cut(s) 56, 781, 856
Alw44I GTGCAC 1 cut(s) 1683
AlwI GGATC 3 cut(s) 682, 700, 1773
AlwNI CAGNNNCTG 1 cut(s) 1599
Ama87I CYCGRG 1 cut(s) 86
ApaLI GTGCAC 1 cut(s) 1683
ApeKI GCWGC 4 cut(s) 815, 1063, 1262, 1377
ApoI RAATTY 3 cut(s) 294, 727, 1025
ArsI GACNNNNNNTTYG 2 cut(s) 285, 317
Asp700I GAANNNNTTC 1 cut(s) 474
Asp718I GGTACC 1 cut(s) 550
AspA2I CCTAGG 1 cut(s) 1814
AspLEI GCGC 2 cut(s) 489, 968
AsuC2I CCSGG 1 cut(s) 275
AsuHPI GGTGA 4 cut(s) 241, 445, 1204, 1643
AvaI CYCGRG 1 cut(s) 86
AvrII CCTAGG 1 cut(s) 1814
BaeGI GKGCMC 5 cut(s) 193, 1460, 1573, 1687, 1814
BanI GGYRCC 5 cut(s) 486, 529, 550, 1809, 1869
BanII GRGCYC 3 cut(s) 8, 87, 386
BauI CACGAG 1 cut(s) 192
BbsI GAAGAC 1 cut(s) 1844
Bbv12I GWGCWC 5 cut(s) 87, 147, 386, 941, 1687
BbvI GCAGC 4 cut(s) 802, 1050, 1274, 1364
BccI CCATC 5 cut(s) 477, 715, 854, 935, 1315
BciT130I CCWGG 5 cut(s) 159, 211, 617, 1461, 1710
BcnI CCSGG 1 cut(s) 275
BcoDI GTCTC 3 cut(s) 56, 781, 856
BcuI ACTAGT 1 cut(s) 790
BfaI CTAG 4 cut(s) 116, 791, 1103, 1815
BfmI CTRYAG 2 cut(s) 1064, 1471
BfoI RGCGCY 2 cut(s) 490, 969
BfrI CTTAAG 1 cut(s) 242
BfuAI ACCTGC 1 cut(s) 1857
BisI GCNGC 4 cut(s) 816, 1064, 1263, 1378
BlnI CCTAGG 1 cut(s) 1814
BlsI GCNGC 4 cut(s) 817, 1065, 1264, 1379
BmcAI AGTACT 2 cut(s) 114, 1915
Bme1390I CCNGG 6 cut(s) 159, 211, 275, 617, 1461, 1710
BmeT110I CYCGRG 1 cut(s) 86
BmiI GGNNCC 6 cut(s) 488, 531, 552, 1240, 1811, 1871
BmrFI CCNGG 6 cut(s) 159, 211, 275, 617, 1461, 1710
BmrI ACTGGG 3 cut(s) 104, 515, 767
BmsI GCATC 7 cut(s) 753, 1027, 1030, 1430, 1483, 1531, 1714
BmuI ACTGGG 3 cut(s) 104, 515, 767
BpiI GAAGAC 1 cut(s) 1844
BpuEI CTTGAG 1 cut(s) 1827
BpuMI CCSGG 1 cut(s) 275
BsaBI GATNNNNATC 1 cut(s) 989
BsaHI GRCGYC 1 cut(s) 487
BsaJI CCNNGG 5 cut(s) 616, 1058, 1459, 1700, 1814
BsaXI ACNNNNNCTCC 2 cut(s) 1039, 1069
Bsc4I CCNNNNNNNGG 1 cut(s) 1465
Bse1I ACTGG 8 cut(s) 110, 510, 643, 773, 833, 986, 1879, 1930
Bse3DI GCAATG 2 cut(s) 966, 1563
Bse8I GATNNNNATC 1 cut(s) 989
BseBI CCWGG 5 cut(s) 159, 211, 617, 1461, 1710
BseDI CCNNGG 5 cut(s) 616, 1058, 1459, 1700, 1814
BseGI GGATG 6 cut(s) 123, 355, 1045, 1421, 1705, 1898
BseJI GATNNNNATC 1 cut(s) 989
BseLI CCNNNNNNNGG 1 cut(s) 1465
BseMI GCAATG 2 cut(s) 966, 1563
BseMII CTCAG 3 cut(s) 132, 162, 722
BseNI ACTGG 8 cut(s) 110, 510, 643, 773, 833, 986, 1879, 1930
BseRI GAGGAG 2 cut(s) 7, 1739
BseSI GKGCMC 5 cut(s) 193, 1460, 1573, 1687, 1814
BseXI GCAGC 4 cut(s) 802, 1050, 1274, 1364
BseYI CCCAGC 1 cut(s) 127
BshNI GGYRCC 5 cut(s) 486, 529, 550, 1809, 1869
BsiHKAI GWGCWC 5 cut(s) 87, 147, 386, 941, 1687
BsiHKCI CYCGRG 1 cut(s) 86
BsiSI CCGG 1 cut(s) 275
BslFI GGGAC 3 cut(s) 877, 1644, 1682
BslI CCNNNNNNNGG 1 cut(s) 1465
BsmAI GTCTC 3 cut(s) 56, 781, 856
BsmFI GGGAC 3 cut(s) 877, 1644, 1682
BsoBI CYCGRG 1 cut(s) 86
Bsp143I GATC 4 cut(s) 246, 674, 692, 1765
BspACI CCGC 5 cut(s) 21, 809, 1236, 1763, 1807
BspCNI CTCAG 3 cut(s) 133, 161, 721
BspHI TCATGA 1 cut(s) 597
BspLI GGNNCC 6 cut(s) 488, 531, 552, 1240, 1811, 1871
BspMAI CTGCAG 2 cut(s) 1068, 1475
BspMI ACCTGC 1 cut(s) 1857
BspPI GGATC 3 cut(s) 682, 700, 1773
BspQI GCTCTTC 1 cut(s) 13
BspT107I GGYRCC 5 cut(s) 486, 529, 550, 1809, 1869
BspTI CTTAAG 1 cut(s) 242
BsrDI GCAATG 2 cut(s) 966, 1563
BsrI ACTGG 8 cut(s) 110, 510, 643, 773, 833, 986, 1879, 1930
BssECI CCNNGG 5 cut(s) 616, 1058, 1459, 1700, 1814
BssMI GATC 4 cut(s) 246, 674, 692, 1765
BssNI GRCGYC 1 cut(s) 487
BssSI CACGAG 1 cut(s) 192
BssT1I CCWWGG 3 cut(s) 1058, 1700, 1814
Bst2BI CACGAG 1 cut(s) 192
Bst2UI CCWGG 5 cut(s) 159, 211, 617, 1461, 1710
Bst4CI ACNGT 5 cut(s) 463, 868, 1586, 1609, 1677
Bst6I CTCTTC 6 cut(s) 13, 617, 753, 1207, 1249, 1255
BstACI GRCGYC 1 cut(s) 487
BstAFI CTTAAG 1 cut(s) 242
BstDEI CTNAG 5 cut(s) 141, 148, 708, 752, 1112
BstF5I GGATG 6 cut(s) 123, 355, 1045, 1421, 1705, 1898
BstH2I RGCGCY 2 cut(s) 490, 969
BstHHI GCGC 2 cut(s) 489, 968
BstKTI GATC 4 cut(s) 249, 677, 695, 1768
BstMAI GTCTC 3 cut(s) 56, 781, 856
BstMBI GATC 4 cut(s) 246, 674, 692, 1765
BstMWI GCNNNNNNNGC 7 cut(s) 815, 965, 1271, 1280, 1401, 1470, 1818
BstNI CCWGG 5 cut(s) 159, 211, 617, 1461, 1710
BstNSI RCATGY 1 cut(s) 1418
BstSCI CCNGG 6 cut(s) 157, 209, 273, 615, 1459, 1708
BstSFI CTRYAG 2 cut(s) 1064, 1471
BstSLI GKGCMC 5 cut(s) 193, 1460, 1573, 1687, 1814
BstV1I GCAGC 4 cut(s) 802, 1050, 1274, 1364
BstV2I GAAGAC 1 cut(s) 1844
BstX2I RGATCY 2 cut(s) 692, 1765
BstXI CCANNNNNNTGG 1 cut(s) 1417
BstYI RGATCY 2 cut(s) 692, 1765
BtsCI GGATG 6 cut(s) 123, 355, 1045, 1421, 1705, 1898
BtsIMutI CAGTG 5 cut(s) 344, 503, 684, 716, 1600
BveI ACCTGC 1 cut(s) 1857
CaiI CAGNNNCTG 1 cut(s) 1599
CciI TCATGA 1 cut(s) 597
CfoI GCGC 2 cut(s) 489, 968
Csp6I GTAC 5 cut(s) 113, 551, 826, 1162, 1914
CspCI CAANNNNNGTGG 2 cut(s) 1781, 1816
CviAII CATG 4 cut(s) 391, 598, 1415, 1919
CviQI GTAC 5 cut(s) 113, 551, 826, 1162, 1914
DdeI CTNAG 5 cut(s) 141, 148, 708, 752, 1112
DinI GGCGCC 1 cut(s) 488
DpnI GATC 4 cut(s) 248, 676, 694, 1767
DpnII GATC 4 cut(s) 246, 674, 692, 1765
DrdI GACNNNNNNGTC 2 cut(s) 1098, 1584
DseDI GACNNNNNNGTC 2 cut(s) 1098, 1584
Eam1104I CTCTTC 6 cut(s) 13, 617, 753, 1207, 1249, 1255
EarI CTCTTC 6 cut(s) 13, 617, 753, 1207, 1249, 1255
EciI GGCGGA 2 cut(s) 10, 1778
Ecl136II GAGCTC 2 cut(s) 85, 384
Eco130I CCWWGG 3 cut(s) 1058, 1700, 1814
Eco24I GRGCYC 3 cut(s) 8, 87, 386
Eco53kI GAGCTC 2 cut(s) 85, 384
Eco57I CTGAAG 1 cut(s) 350
Eco88I CYCGRG 1 cut(s) 86
EcoICRI GAGCTC 2 cut(s) 85, 384
EcoRII CCWGG 5 cut(s) 157, 209, 615, 1459, 1708
EcoT14I CCWWGG 3 cut(s) 1058, 1700, 1814
EcoT22I ATGCAT 1 cut(s) 768
EcoT38I GRGCYC 3 cut(s) 8, 87, 386
EgeI GGCGCC 1 cut(s) 488
EheI GGCGCC 1 cut(s) 488
ErhI CCWWGG 3 cut(s) 1058, 1700, 1814
FaeI CATG 4 cut(s) 394, 601, 1418, 1922
FalI AAGNNNNNCTT 2 cut(s) 236, 268
FaqI GGGAC 3 cut(s) 877, 1644, 1682
FatI CATG 4 cut(s) 390, 597, 1414, 1918
FauI CCCGC 1 cut(s) 802
FblI GTMKAC 1 cut(s) 1090
Fnu4HI GCNGC 4 cut(s) 816, 1064, 1263, 1378
FokI GGATG 6 cut(s) 110, 362, 1052, 1408, 1692, 1905
FriOI GRGCYC 3 cut(s) 8, 87, 386
Fsp4HI GCNGC 4 cut(s) 816, 1064, 1263, 1378
FspBI CTAG 4 cut(s) 116, 791, 1103, 1815
GlaI GCGC 2 cut(s) 488, 967
GluI GCNGC 4 cut(s) 816, 1064, 1263, 1378
GsaI CCCAGC 1 cut(s) 131
HaeII RGCGCY 2 cut(s) 490, 969
HapII CCGG 1 cut(s) 275
HhaI GCGC 2 cut(s) 489, 968
Hin1I GRCGYC 1 cut(s) 487
Hin1II CATG 4 cut(s) 394, 601, 1418, 1922
Hin6I GCGC 2 cut(s) 487, 966
HinP1I GCGC 2 cut(s) 487, 966
HincII GTYRAC 1 cut(s) 1354
HindII GTYRAC 1 cut(s) 1354
HinfI GANTC 7 cut(s) 446, 1054, 1120, 1139, 1154, 1578, 1840
HpaII CCGG 1 cut(s) 275
HphI GGTGA 4 cut(s) 241, 445, 1204, 1643
Hpy166II GTNNAC 8 cut(s) 185, 239, 258, 1091, 1164, 1354, 1384, 1685
Hpy188III TCNNGA 2 cut(s) 598, 944
Hpy8I GTNNAC 8 cut(s) 185, 239, 258, 1091, 1164, 1354, 1384, 1685
HpyAV CCTTC 4 cut(s) 636, 1063, 1399, 1622
HpyCH4III ACNGT 5 cut(s) 463, 868, 1586, 1609, 1677
HpyF10VI GCNNNNNNNGC 7 cut(s) 815, 965, 1271, 1280, 1401, 1470, 1818
HpyF3I CTNAG 5 cut(s) 141, 148, 708, 752, 1112
Hsp92I GRCGYC 1 cut(s) 487
Hsp92II CATG 4 cut(s) 394, 601, 1418, 1922
HspAI GCGC 2 cut(s) 487, 966
KasI GGCGCC 1 cut(s) 486
KpnI GGTACC 1 cut(s) 554
Kzo9I GATC 4 cut(s) 246, 674, 692, 1765
LguI GCTCTTC 1 cut(s) 13
LmnI GCTCC 4 cut(s) 381, 686, 812, 1898
Lsp1109I GCAGC 4 cut(s) 802, 1050, 1274, 1364
LweI GCATC 7 cut(s) 753, 1027, 1030, 1430, 1483, 1531, 1714
MaeI CTAG 4 cut(s) 116, 791, 1103, 1815
MaeIII GTNAC 2 cut(s) 451, 1595
MalI GATC 4 cut(s) 248, 676, 694, 1767
MboI GATC 4 cut(s) 246, 674, 692, 1765
MfeI CAATTG 2 cut(s) 534, 544
MflI RGATCY 2 cut(s) 692, 1765
MluCI AATT 7 cut(s) 294, 534, 544, 727, 798, 887, 1025
Mly113I GGCGCC 1 cut(s) 487
MlyI GAGTC 6 cut(s) 440, 1063, 1129, 1133, 1572, 1834
MmeI TCCRAC 3 cut(s) 86, 1017, 1317
Mph1103I ATGCAT 1 cut(s) 768
MroXI GAANNNNTTC 1 cut(s) 474
MseI TTAA 5 cut(s) 215, 243, 1149, 1740, 1776
MslI CAYNNNNRTG 1 cut(s) 572
MspA1I CMGCKG 1 cut(s) 152
MspCI CTTAAG 1 cut(s) 242
MspI CCGG 1 cut(s) 275
MspR9I CCNGG 6 cut(s) 159, 211, 275, 617, 1461, 1710
MunI CAATTG 2 cut(s) 534, 544
MvaI CCWGG 5 cut(s) 159, 211, 617, 1461, 1710
MwoI GCNNNNNNNGC 7 cut(s) 815, 965, 1271, 1280, 1401, 1470, 1818
NarI GGCGCC 1 cut(s) 487
NciI CCSGG 1 cut(s) 275
NdeII GATC 4 cut(s) 246, 674, 692, 1765
NlaIII CATG 4 cut(s) 394, 601, 1418, 1922
NlaIV GGNNCC 6 cut(s) 488, 531, 552, 1240, 1811, 1871
NmuCI GTSAC 2 cut(s) 451, 1595
NsiI ATGCAT 1 cut(s) 768
NspI RCATGY 1 cut(s) 1418
PaeR7I CTCGAG 1 cut(s) 86
PagI TCATGA 1 cut(s) 597
PaqCI CACCTGC 1 cut(s) 1857
PciSI GCTCTTC 1 cut(s) 13
PdmI GAANNNNTTC 1 cut(s) 474
PfeI GAWTC 1 cut(s) 1154
PflFI GACNNNGTC 2 cut(s) 449, 1095
PfoI TCCNGGA 2 cut(s) 273, 1708
PkrI GCNGC 4 cut(s) 817, 1065, 1264, 1379
PleI GAGTC 6 cut(s) 440, 1062, 1128, 1133, 1572, 1834
PluTI GGCGCC 1 cut(s) 490
PpsI GAGTC 6 cut(s) 440, 1062, 1128, 1133, 1572, 1834
Psp124BI GAGCTC 2 cut(s) 87, 386
Psp6I CCWGG 5 cut(s) 157, 209, 615, 1459, 1708
PspFI CCCAGC 1 cut(s) 127
PspGI CCWGG 5 cut(s) 157, 209, 615, 1459, 1708
PspN4I GGNNCC 6 cut(s) 488, 531, 552, 1240, 1811, 1871
PspXI VCTCGAGB 1 cut(s) 86
PstI CTGCAG 2 cut(s) 1068, 1475
PstNI CAGNNNCTG 1 cut(s) 1599
PsuI RGATCY 2 cut(s) 692, 1765
PsyI GACNNNGTC 2 cut(s) 449, 1095
PvuII CAGCTG 1 cut(s) 152
RsaI GTAC 5 cut(s) 114, 552, 827, 1163, 1915
RsaNI GTAC 5 cut(s) 113, 551, 826, 1162, 1914
RseI CAYNNNNRTG 1 cut(s) 572
SacI GAGCTC 2 cut(s) 87, 386
SapI GCTCTTC 1 cut(s) 13
SaqAI TTAA 5 cut(s) 215, 243, 1149, 1740, 1776
SatI GCNGC 4 cut(s) 816, 1064, 1263, 1378
Sau3AI GATC 4 cut(s) 246, 674, 692, 1765
ScaI AGTACT 2 cut(s) 114, 1915
SchI GAGTC 6 cut(s) 440, 1063, 1129, 1133, 1572, 1834
ScrFI CCNGG 6 cut(s) 159, 211, 275, 617, 1461, 1710
SfaNI GCATC 7 cut(s) 753, 1027, 1030, 1430, 1483, 1531, 1714
SfcI CTRYAG 2 cut(s) 1064, 1471
SfoI GGCGCC 1 cut(s) 488
Sfr274I CTCGAG 1 cut(s) 86
SlaI CTCGAG 1 cut(s) 86
SmiMI CAYNNNNRTG 1 cut(s) 572
SmlI CTYRAG 3 cut(s) 86, 242, 1842
SmoI CTYRAG 3 cut(s) 86, 242, 1842
SpeI ACTAGT 1 cut(s) 790
Sse9I AATT 7 cut(s) 294, 534, 544, 727, 798, 887, 1025
SsiI CCGC 5 cut(s) 21, 809, 1236, 1763, 1807
SspDI GGCGCC 1 cut(s) 486
SspI AATATT 1 cut(s) 1826
SspMI CTAG 4 cut(s) 116, 791, 1103, 1815
SstI GAGCTC 2 cut(s) 87, 386
StyD4I CCNGG 6 cut(s) 157, 209, 273, 615, 1459, 1708
StyI CCWWGG 3 cut(s) 1058, 1700, 1814
TaaI ACNGT 5 cut(s) 463, 868, 1586, 1609, 1677
TaqI TCGA 3 cut(s) 87, 945, 1223
TasI AATT 7 cut(s) 294, 534, 544, 727, 798, 887, 1025
TatI WGTACW 3 cut(s) 112, 1161, 1913
TfiI GAWTC 1 cut(s) 1154
Tru1I TTAA 5 cut(s) 215, 243, 1149, 1740, 1776
Tru9I TTAA 5 cut(s) 215, 243, 1149, 1740, 1776
TscAI CASTG 5 cut(s) 344, 510, 684, 723, 1600
TseFI GTSAC 2 cut(s) 451, 1595
TseI GCWGC 4 cut(s) 815, 1063, 1262, 1377
Tsp45I GTSAC 2 cut(s) 451, 1595
TspDTI ATGAA 6 cut(s) 299, 418, 420, 638, 1019, 1242
TspRI CASTG 5 cut(s) 344, 510, 684, 723, 1600
Tth111I GACNNNGTC 2 cut(s) 449, 1095
Vha464I CTTAAG 1 cut(s) 242
VneI GTGCAC 1 cut(s) 1683
XapI RAATTY 3 cut(s) 294, 727, 1025
XceI RCATGY 1 cut(s) 1418
XcmI CCANNNNNNNNNTGG 1 cut(s) 1886
XhoI CTCGAG 1 cut(s) 86
XmaJI CCTAGG 1 cut(s) 1814
XmiI GTMKAC 1 cut(s) 1090
XmnI GAANNNNTTC 1 cut(s) 474
XspI CTAG 4 cut(s) 116, 791, 1103, 1815
ZrmI AGTACT 2 cut(s) 114, 1915
Zsp2I ATGCAT 1 cut(s) 768
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.