FvH4_1g28540

NEMP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0004377
Physical Location & Seq
Forward (+)
20751606 .. 20758856
7251 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g28540.t3

Sequence Viewer

Length: 1443 bp
ATGGCCCTACATCTCCTCTTCGTTTTCAGTCTCTTTATCTCATTCTTATGTTCATCAACTGGCGGGATCACTCTCAAAGGTATTGATATTGAAAACCCAGTAATTAATGTCAACCCGGCACCGGTTTCTGGTTCCAAAGATGCTTTATTCTGTGAACGAGTTCGAGTATCTGGTATATCTAGATCAAATCTTGCGAGTTATGCTAATTCCTTTCGGATTTCGCTGTCTCCATCTGTCGTAATCCCAGAGAGACTGCATAGCAGAATTGCTATCTGTTTTCACAGTAATGCCTCGCTTGGACTCTGTCAGTGTAGAAAGGATGATTGGAAAACTCTTGAGAATGGGTTATGGGAATCTGTCATGTCCCCTTATCAGGAAAAATATGTTGATGTCAAGTACATTGGCAATTCACTTGGGTGTTCCACTCTCACTGTTGAACAAGTCTCTCAGCAATGGCGCCTATTGTTTCTAGCCATGGGATTCAGTTTACTGTTGCTGGCACCGAGCATCAGTAGTTGGGTTCATTTTTATTACAGCACTTCAATGGTTATAGGAGTTTTTATGGTCATTATATTCCTTCTCTTCCAGGGAATGAAATTGCTGCCATTTGGAAGAAAAAATTTCTTCTATCTTGTTACTATATATGGATCAGTGCTTGGAGCTGGATCTTATGTTTTTCAGCACTTCTCTGCCATGGTAAATTCATTTCTTGTGAATTTTGGGATAAGTGAAGACATGCACAATCTGATCTCAAAATTTTTACTGGTTGCAATTATCGTTACGGGTGCTGCACTAGGTTACTGGATTGGGAGGAAGTTTTTCATCTCAAAAGATGGAACAGTAGATGTTTCTGTAGCCCAATTTGCTATGTGGGCAATACGCTTTTTCGGAATCACGTTCATCTCTCAGAGCACTCTCGATACACCTTTGGCTATTGGGGCGTTGGTTTCCTGCTTGGGTATCTGCAAGCTTATCACTTCTCTGAAATGGCATGGCAGAATGAACCAGTCAAATGGTGCGAGTGGGAGACTTCTACTGCAGAAAAGAAGACACAGTCGTTCAGAGTTCCTTAGTAGGTCCAGCCCACTAGAGGCATGGAACAGTACAAGGGATGCATCTGCTTGGTCTGATTCTCCTGTTAAAGGTGTTGTATCGCCATCTTTTAGGGCATGTAATCAACAGGAGTACTATTCAACCTTCCACAAAATGCGAAACCAAAAGTTCACAAAGAAAGAGTGGGATGAGTTTACACGGGAATCAACGCGACATGCTCTAGCAGAATGGGCAGCATCTCCTGAATTCACTGATTGGGTTATGGAGCATGCCGACCGCATTCAACTCCTTCCAAGCGATAGTTCAGATGAAACTGTGGGAAGTGATTCTGATTCTACAGATGAGAATGACGTGGGTAGCATGGATCGGTGTGGTTTTCTCAAATGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NEMP

Protein Analysis

481

Amino Acids

53.8

Weight (kDa)

8.45

Isoelectric Point (pI)

38.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NEMP PF10225 143 - 391 3.7e-36 NEMP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 118, 456, 499
AccII CGCG 1 cut(s) 1264
AciI CCGC 2 cut(s) 63, 1330
AclWI GGATC 4 cut(s) 74, 655, 673, 1425
AcsI RAATTY 5 cut(s) 619, 700, 715, 755, 1298
AcyI GRCGYC 1 cut(s) 457
AfaI GTAC 3 cut(s) 398, 1105, 1187
AfiI CCNNNNNNNGG 5 cut(s) 121, 128, 373, 1090, 1142
AgeI ACCGGT 1 cut(s) 121
AgsI TTSAA 5 cut(s) 92, 437, 543, 1194, 1337
AjiI CACGTC 1 cut(s) 1405
AjnI CCWGG 1 cut(s) 585
AjuI GAANNNNNNNTTGG 4 cut(s) 307, 339, 1339, 1371
AleI CACNNNNGTG 1 cut(s) 415
AluBI AGCT 2 cut(s) 662, 970
AluI AGCT 2 cut(s) 662, 970
Alw21I GWGCWC 1 cut(s) 914
Alw26I GTCTC 5 cut(s) 35, 231, 244, 448, 1021
AlwI GGATC 4 cut(s) 74, 655, 673, 1425
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 3 cut(s) 601, 788, 1286
ApoI RAATTY 5 cut(s) 619, 700, 715, 755, 1298
AseI ATTAAT 1 cut(s) 105
AsiGI ACCGGT 1 cut(s) 121
Asp700I GAANNNNTTC 3 cut(s) 159, 818, 1378
AspLEI GCGC 1 cut(s) 459
AspS9I GGNCC 2 cut(s) 4, 1077
AsuC2I CCSGG 1 cut(s) 116
AvaII GGWCC 1 cut(s) 1077
BanI GGYRCC 3 cut(s) 118, 456, 499
BbsI GAAGAC 2 cut(s) 738, 1054
Bbv12I GWGCWC 1 cut(s) 914
BbvI GCAGC 3 cut(s) 588, 775, 1298
BccI CCATC 3 cut(s) 238, 827, 1165
BciT130I CCWGG 1 cut(s) 587
BcnI CCSGG 1 cut(s) 116
BcoDI GTCTC 5 cut(s) 35, 231, 244, 448, 1021
BfaI CTAG 5 cut(s) 180, 470, 794, 1088, 1274
BfmI CTRYAG 3 cut(s) 852, 1037, 1389
BfoI RGCGCY 1 cut(s) 460
BisI GCNGC 3 cut(s) 602, 789, 1287
BlsI GCNGC 3 cut(s) 603, 790, 1288
BmcAI AGTACT 1 cut(s) 1187
Bme1390I CCNGG 2 cut(s) 116, 587
Bme18I GGWCC 1 cut(s) 1077
BmgBI CACGTC 1 cut(s) 1405
BmgT120I GGNCC 2 cut(s) 4, 1077
BmiI GGNNCC 4 cut(s) 120, 133, 458, 501
BmrFI CCNGG 2 cut(s) 116, 587
BmrI ACTGGG 1 cut(s) 92
BmsI GCATC 5 cut(s) 130, 516, 1102, 1124, 1298
BmuI ACTGGG 1 cut(s) 92
BpiI GAAGAC 2 cut(s) 738, 1054
BpuEI CTTGAG 1 cut(s) 356
BpuMI CCSGG 1 cut(s) 116
BsaHI GRCGYC 1 cut(s) 457
BsaJI CCNNGG 3 cut(s) 474, 586, 693
BsaWI WCCGGW 1 cut(s) 121
Bsc4I CCNNNNNNNGG 5 cut(s) 121, 128, 373, 1090, 1142
Bse118I RCCGGY 1 cut(s) 121
Bse1I ACTGG 5 cut(s) 64, 98, 768, 806, 1006
Bse3DI GCAATG 1 cut(s) 458
BseBI CCWGG 1 cut(s) 587
BseDI CCNNGG 3 cut(s) 474, 586, 693
BseGI GGATG 3 cut(s) 325, 1117, 1246
BseLI CCNNNNNNNGG 5 cut(s) 121, 128, 373, 1090, 1142
BseMI GCAATG 1 cut(s) 458
BseMII CTCAG 2 cut(s) 461, 920
BseNI ACTGG 5 cut(s) 64, 98, 768, 806, 1006
BseRI GAGGAG 1 cut(s) 5
BseXI GCAGC 3 cut(s) 588, 775, 1298
BsgI GTGCAG 1 cut(s) 774
Bsh1236I CGCG 1 cut(s) 1264
Bsh1285I CGRYCG 1 cut(s) 1330
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 3 cut(s) 118, 456, 499
BshTI ACCGGT 1 cut(s) 121
BsiEI CGRYCG 1 cut(s) 1330
BsiHKAI GWGCWC 1 cut(s) 914
BsiSI CCGG 2 cut(s) 116, 122
BslFI GGGAC 1 cut(s) 349
BslI CCNNNNNNNGG 5 cut(s) 121, 128, 373, 1090, 1142
BsmAI GTCTC 5 cut(s) 35, 231, 244, 448, 1021
BsmFI GGGAC 1 cut(s) 349
BsmI GAATGC 1 cut(s) 1332
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 1 cut(s) 914
Bsp143I GATC 6 cut(s) 66, 182, 647, 665, 747, 1417
Bsp19I CCATGG 2 cut(s) 474, 693
BspACI CCGC 2 cut(s) 63, 1330
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 460, 919
BspFNI CGCG 1 cut(s) 1264
BspLI GGNNCC 4 cut(s) 120, 133, 458, 501
BspMAI CTGCAG 1 cut(s) 1041
BspPI GGATC 4 cut(s) 74, 655, 673, 1425
BspT107I GGYRCC 3 cut(s) 118, 456, 499
BsrDI GCAATG 1 cut(s) 458
BsrFI RCCGGY 1 cut(s) 121
BsrI ACTGG 5 cut(s) 64, 98, 768, 806, 1006
BssAI RCCGGY 1 cut(s) 121
BssECI CCNNGG 3 cut(s) 474, 586, 693
BssMI GATC 6 cut(s) 66, 182, 647, 665, 747, 1417
BssNI GRCGYC 1 cut(s) 457
BssT1I CCWWGG 2 cut(s) 474, 693
Bst2UI CCWGG 1 cut(s) 587
Bst4CI ACNGT 7 cut(s) 284, 433, 492, 841, 1055, 1103, 1369
Bst6I CTCTTC 2 cut(s) 23, 587
BstACI GRCGYC 1 cut(s) 457
BstC8I GCNNGC 3 cut(s) 498, 968, 1323
BstDEI CTNAG 3 cut(s) 447, 906, 1070
BstDSI CCRYGG 2 cut(s) 474, 693
BstENI CCTNNNNNAGG 1 cut(s) 1140
BstF5I GGATG 3 cut(s) 325, 1117, 1246
BstFNI CGCG 1 cut(s) 1264
BstH2I RGCGCY 1 cut(s) 460
BstHHI GCGC 1 cut(s) 459
BstKTI GATC 6 cut(s) 69, 185, 650, 668, 750, 1420
BstMAI GTCTC 5 cut(s) 35, 231, 244, 448, 1021
BstMBI GATC 6 cut(s) 66, 182, 647, 665, 747, 1417
BstMCI CGRYCG 1 cut(s) 1330
BstMWI GCNNNNNNNGC 5 cut(s) 200, 863, 872, 938, 1283
BstNI CCWGG 1 cut(s) 587
BstNSI RCATGY 4 cut(s) 739, 1173, 1271, 1325
BstSCI CCNGG 2 cut(s) 114, 585
BstSFI CTRYAG 3 cut(s) 852, 1037, 1389
BstUI CGCG 1 cut(s) 1264
BstV1I GCAGC 3 cut(s) 588, 775, 1298
BstV2I GAAGAC 2 cut(s) 738, 1054
BstX2I RGATCY 1 cut(s) 665
BstXI CCANNNNNNTGG 1 cut(s) 1013
BstYI RGATCY 1 cut(s) 665
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 2 cut(s) 474, 693
BtrI CACGTC 1 cut(s) 1405
BtsCI GGATG 3 cut(s) 325, 1117, 1246
BtsIMutI CAGTG 4 cut(s) 314, 429, 657, 1302
Cac8I GCNNGC 3 cut(s) 498, 968, 1323
CfoI GCGC 1 cut(s) 459
Cfr10I RCCGGY 1 cut(s) 121
Cfr13I GGNCC 2 cut(s) 4, 1077
Csp6I GTAC 3 cut(s) 397, 1104, 1186
CspAI ACCGGT 1 cut(s) 121
CviJI RGCY 7 cut(s) 5, 473, 662, 857, 932, 970, 1083
CviKI_1 RGCY 7 cut(s) 5, 473, 662, 857, 932, 970, 1083
CviQI GTAC 3 cut(s) 397, 1104, 1186
DdeI CTNAG 3 cut(s) 447, 906, 1070
DinI GGCGCC 1 cut(s) 458
DpnI GATC 6 cut(s) 68, 184, 649, 667, 749, 1419
DpnII GATC 6 cut(s) 66, 182, 647, 665, 747, 1417
Eam1104I CTCTTC 2 cut(s) 23, 587
EarI CTCTTC 2 cut(s) 23, 587
Eco130I CCWWGG 2 cut(s) 474, 693
Eco47I GGWCC 1 cut(s) 1077
EcoNI CCTNNNNNAGG 1 cut(s) 1140
EcoRI GAATTC 1 cut(s) 1298
EcoRII CCWGG 1 cut(s) 585
EcoT14I CCWWGG 2 cut(s) 474, 693
EcoT22I ATGCAT 1 cut(s) 1117
EgeI GGCGCC 1 cut(s) 458
EheI GGCGCC 1 cut(s) 458
ErhI CCWWGG 2 cut(s) 474, 693
FaqI GGGAC 1 cut(s) 349
FauI CCCGC 1 cut(s) 56
Fnu4HI GCNGC 3 cut(s) 602, 789, 1287
FokI GGATG 3 cut(s) 332, 1124, 1253
Fsp4HI GCNGC 3 cut(s) 602, 789, 1287
FspBI CTAG 5 cut(s) 180, 470, 794, 1088, 1274
GlaI GCGC 1 cut(s) 458
GluI GCNGC 3 cut(s) 602, 789, 1287
HaeII RGCGCY 1 cut(s) 460
HaeIII GGCC 1 cut(s) 5
HapII CCGG 2 cut(s) 116, 122
HhaI GCGC 1 cut(s) 459
Hin1I GRCGYC 1 cut(s) 457
Hin6I GCGC 1 cut(s) 457
HinP1I GCGC 1 cut(s) 457
HincII GTYRAC 1 cut(s) 112
HindII GTYRAC 1 cut(s) 112
HindIII AAGCTT 1 cut(s) 968
HinfI GANTC 8 cut(s) 300, 353, 480, 891, 1130, 1256, 1379, 1385
HpaII CCGG 2 cut(s) 116, 122
Hpy166II GTNNAC 5 cut(s) 112, 155, 488, 1224, 1248
Hpy188I TCNGA 9 cut(s) 216, 747, 890, 909, 984, 1063, 1129, 1360, 1384
Hpy188III TCNNGA 5 cut(s) 180, 335, 374, 917, 1295
Hpy8I GTNNAC 5 cut(s) 112, 155, 488, 1224, 1248
HpyAV CCTTC 3 cut(s) 587, 1207, 1352
HpyCH4III ACNGT 7 cut(s) 284, 433, 492, 841, 1055, 1103, 1369
HpyCH4IV ACGT 2 cut(s) 896, 1404
HpyCH4V TGCA 7 cut(s) 256, 739, 770, 791, 966, 1039, 1115
HpyF10VI GCNNNNNNNGC 5 cut(s) 200, 863, 872, 938, 1283
HpyF3I CTNAG 3 cut(s) 447, 906, 1070
HpySE526I ACGT 2 cut(s) 896, 1404
Hsp92I GRCGYC 1 cut(s) 457
HspAI GCGC 1 cut(s) 457
KasI GGCGCC 1 cut(s) 456
Kzo9I GATC 6 cut(s) 66, 182, 647, 665, 747, 1417
LmnI GCTCC 2 cut(s) 659, 1318
Lsp1109I GCAGC 3 cut(s) 588, 775, 1298
LweI GCATC 5 cut(s) 130, 516, 1102, 1124, 1298
MaeI CTAG 5 cut(s) 180, 470, 794, 1088, 1274
MaeII ACGT 2 cut(s) 896, 1404
MaeIII GTNAC 3 cut(s) 634, 778, 797
MalI GATC 6 cut(s) 68, 184, 649, 667, 749, 1419
MboI GATC 6 cut(s) 66, 182, 647, 665, 747, 1417
MboII GAAGA 6 cut(s) 10, 574, 616, 624, 743, 1059
MflI RGATCY 1 cut(s) 665
MhlI GDGCHC 1 cut(s) 914
Mly113I GGCGCC 1 cut(s) 457
MlyI GAGTC 1 cut(s) 294
MnlI CCTC 4 cut(s) 26, 301, 804, 1084
Mph1103I ATGCAT 1 cut(s) 1117
MroXI GAANNNNTTC 3 cut(s) 159, 818, 1378
MseI TTAA 2 cut(s) 105, 1140
MslI CAYNNNNRTG 4 cut(s) 46, 285, 415, 542
MspI CCGG 2 cut(s) 116, 122
MspR9I CCNGG 2 cut(s) 116, 587
Mva1269I GAATGC 1 cut(s) 1332
MvaI CCWGG 1 cut(s) 587
MvnI CGCG 1 cut(s) 1264
MwoI GCNNNNNNNGC 5 cut(s) 200, 863, 872, 938, 1283
NarI GGCGCC 1 cut(s) 457
NciI CCSGG 1 cut(s) 116
NcoI CCATGG 2 cut(s) 474, 693
NdeII GATC 6 cut(s) 66, 182, 647, 665, 747, 1417
NlaIV GGNNCC 4 cut(s) 120, 133, 458, 501
NsiI ATGCAT 1 cut(s) 1117
NspI RCATGY 4 cut(s) 739, 1173, 1271, 1325
OliI CACNNNNGTG 1 cut(s) 415
PaeI GCATGC 1 cut(s) 1325
PctI GAATGC 1 cut(s) 1332
PdmI GAANNNNTTC 3 cut(s) 159, 818, 1378
PfeI GAWTC 7 cut(s) 353, 480, 891, 1130, 1256, 1379, 1385
PflFI GACNNNGTC 2 cut(s) 303, 1053
PinAI ACCGGT 1 cut(s) 121
PkrI GCNGC 3 cut(s) 603, 790, 1288
PleI GAGTC 1 cut(s) 294
PluTI GGCGCC 1 cut(s) 460
PpsI GAGTC 1 cut(s) 294
PshBI ATTAAT 1 cut(s) 105
Psp6I CCWGG 1 cut(s) 585
PspGI CCWGG 1 cut(s) 585
PspN4I GGNNCC 4 cut(s) 120, 133, 458, 501
PspPI GGNCC 2 cut(s) 4, 1077
PstI CTGCAG 1 cut(s) 1041
PsuI RGATCY 1 cut(s) 665
PsyI GACNNNGTC 2 cut(s) 303, 1053
RsaI GTAC 3 cut(s) 398, 1105, 1187
RsaNI GTAC 3 cut(s) 397, 1104, 1186
RseI CAYNNNNRTG 4 cut(s) 46, 285, 415, 542
SaqAI TTAA 2 cut(s) 105, 1140
SatI GCNGC 3 cut(s) 602, 789, 1287
Sau3AI GATC 6 cut(s) 66, 182, 647, 665, 747, 1417
Sau96I GGNCC 2 cut(s) 4, 1077
ScaI AGTACT 1 cut(s) 1187
SchI GAGTC 1 cut(s) 294
ScrFI CCNGG 2 cut(s) 116, 587
SduI GDGCHC 1 cut(s) 914
SfaNI GCATC 5 cut(s) 130, 516, 1102, 1124, 1298
SfcI CTRYAG 3 cut(s) 852, 1037, 1389
SfoI GGCGCC 1 cut(s) 458
SinI GGWCC 1 cut(s) 1077
SmiMI CAYNNNNRTG 4 cut(s) 46, 285, 415, 542
SmlI CTYRAG 1 cut(s) 335
SmoI CTYRAG 1 cut(s) 335
SphI GCATGC 1 cut(s) 1325
SsiI CCGC 2 cut(s) 63, 1330
SspDI GGCGCC 1 cut(s) 456
SspMI CTAG 5 cut(s) 180, 470, 794, 1088, 1274
StyD4I CCNGG 2 cut(s) 114, 585
StyI CCWWGG 2 cut(s) 474, 693
TaaI ACNGT 7 cut(s) 284, 433, 492, 841, 1055, 1103, 1369
TaiI ACGT 2 cut(s) 899, 1407
TaqI TCGA 2 cut(s) 163, 918
TatI WGTACW 3 cut(s) 396, 1103, 1185
TfiI GAWTC 7 cut(s) 353, 480, 891, 1130, 1256, 1379, 1385
Tru1I TTAA 2 cut(s) 105, 1140
Tru9I TTAA 2 cut(s) 105, 1140
TscAI CASTG 4 cut(s) 314, 436, 657, 1309
TseI GCWGC 3 cut(s) 601, 788, 1286
TspDTI ATGAA 8 cut(s) 42, 512, 608, 693, 811, 889, 1016, 1377
TspRI CASTG 4 cut(s) 314, 436, 657, 1309
Tth111I GACNNNGTC 2 cut(s) 303, 1053
VpaK11BI GGWCC 1 cut(s) 1077
VspI ATTAAT 1 cut(s) 105
XagI CCTNNNNNAGG 1 cut(s) 1140
XapI RAATTY 5 cut(s) 619, 700, 715, 755, 1298
XbaI TCTAGA 1 cut(s) 179
XceI RCATGY 4 cut(s) 739, 1173, 1271, 1325
XcmI CCANNNNNNNNNTGG 1 cut(s) 1092
XmnI GAANNNNTTC 3 cut(s) 159, 818, 1378
XspI CTAG 5 cut(s) 180, 470, 794, 1088, 1274
ZrmI AGTACT 1 cut(s) 1187
Zsp2I ATGCAT 1 cut(s) 1117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.