RchiOBHm_Chr3g0496221

NEMP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0004377
Physical Location & Seq
Reverse (-)
46890849 .. 46898960
8112 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45856

Sequence Viewer

Length: 1494 bp
ATGGCTACCCTTCCTCTCTTCGTTTTCTCTCTCTTTCTTTCGACCATCTCATTCTTATGTTCCACTACCCCTACCCCTACCGGGAAAGGCATTGATCTTGAAAACCCAGTACTAAATGTCAACCCAGCACCGCTTTCTCTTTCTGGGCACTTGCCTACCGCTGGTTCTAAAGATGTTTTATTTTGTGAACGGGTTCGAGTTTCTGGTATACCTAGATCAAAAATTGGGAGTTATGCAAATTCCTTTCGGATTACTCTCTCTCCATCTGTCGTAATCCCAGAGAGGTTGCATAGCAAAATTGAGGTCTGTTTTCACCGTAATGATTCGCTTGGACTCTGTCAGTGTAGAAATGATGAATGGAAAAATCTCGAGAAAGGGCTATGGGAATCTGTCATGTCCCCTTATGAGGAGAAATATGTTGATGTCAAGTACATTGGCAATTTACTTGGGTCTTCTAGCATCACTGTTGAAGAAGTTTCTCAGCAATGGCGCCTTTGGTTTCTAGCAGTGGGATTCATTTTACTGTTGCTGGCACCAAGCATCAGTAGTTGGGTTCATTTTTATTATAGCACTTCAATGGTCATAGGAGTTTTTATGGTCATTATATTCCTTCTCTTCCAGGGAATGAAATTGCTGCCATTTGGAAGAAAAAATTTCTTCTATCTTGTTACTGTATATGGATCAGTGCTTGGAGCCGGATCTTATCTCTTTCATCACTTCTCTGCCATGGTAAATTCAATTCTTGTAAATTTTGGGATTAGTGAAGACATGCACAATCTGATCTCTAAATTTTTACTGGTGGCGATTATCCTTATGGGTGCTGCACTAGGTTACTGGATTGGGAGGAAGCTTTTCATCTCAAAGGATGGAACAGTAGATGTTGTTGTAGCCCAATTTGCTATGTGGGCAATCCGCTTTTTTGGAATCACATTCATCTCTCAGAGCACTCTCGATACTCCTTTGGCTATTGGAGCGTTGGTTTCCTGCTTGGTTATCTGCAAACTTATCACTTCTGTGAAGTGGCATGGTAGAATGAATCAGTCAAATGGTGCGAGTGGGAGTCCTTTACAGCAGAAAGGAAGACACGTCACAGGAAGACACAGTCGTCCAGAGTTCCTTTGCAGGTCAAGCGCACTAGAGGCATGGAACAGTACAAGGGGTGCATCTGCTTGGTCTGATTCTCCTGTTAAAGGTGTTGTGTCGCCATCTTTTAGGACATGTGATCAACAGGAGTACTATTCAACCTTCCACAAGATGCGAAACCGAAAGAAGTTCACAAAGAAAGAGTGGGATGATTTTACAAAGGAATCAACACGGCATGCTCTAGCAGAATGGGCAGCATCTCCTGAATTCACCGATTGGATTATCGAGCATGCCGACCGCATACAACTCCTTCCAAGCGATAGTTCAGATGAAACTGTGGGTAGTGAATCTGATTCTACTGATGAGAATGTCGTGGGGAGCACGGATAGGTGTGGTTTTCTCAAATGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NEMP

Protein Analysis

497

Amino Acids

55.6

Weight (kDa)

8.43

Isoelectric Point (pI)

38.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NEMP PF10225 154 - 342 5e-38 NEMP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1104
Acc36I ACCTGC 1 cut(s) 1113
AccB1I GGYRCC 2 cut(s) 489, 532
AccI GTMKAC 1 cut(s) 208
AciI CCGC 4 cut(s) 131, 159, 913, 1381
AclWI GGATC 2 cut(s) 688, 706
AcsI RAATTY 6 cut(s) 238, 652, 733, 748, 788, 1349
AcyI GRCGYC 1 cut(s) 490
AfaI GTAC 4 cut(s) 111, 431, 1153, 1235
AfiI CCNNNNNNNGG 4 cut(s) 81, 161, 406, 1190
AflIII ACRYGT 2 cut(s) 1084, 1217
AgsI TTSAA 5 cut(s) 101, 470, 576, 738, 1242
AjiI CACGTC 1 cut(s) 1087
AjnI CCWGG 1 cut(s) 618
AjuI GAANNNNNNNTTGG 2 cut(s) 1390, 1422
AleI CACNNNNGTG 1 cut(s) 1013
AluBI AGCT 1 cut(s) 850
AluI AGCT 1 cut(s) 850
Alw21I GWGCWC 2 cut(s) 947, 1466
AlwI GGATC 2 cut(s) 688, 706
Ama87I CYCGRG 1 cut(s) 368
ApeKI GCWGC 3 cut(s) 634, 821, 1337
ApoI RAATTY 6 cut(s) 238, 652, 733, 748, 788, 1349
ArsI GACNNNNNNTTYG 2 cut(s) 288, 320
Asp700I GAANNNNTTC 2 cut(s) 192, 851
AspLEI GCGC 2 cut(s) 492, 1133
AsuC2I CCSGG 1 cut(s) 82
AsuHPI GGTGA 2 cut(s) 305, 1345
AvaI CYCGRG 1 cut(s) 368
BaeGI GKGCMC 1 cut(s) 150
BanI GGYRCC 2 cut(s) 489, 532
BbsI GAAGAC 4 cut(s) 444, 771, 1087, 1102
Bbv12I GWGCWC 2 cut(s) 947, 1466
BbvI GCAGC 3 cut(s) 621, 808, 1349
BccI CCATC 4 cut(s) 53, 271, 860, 1213
BceAI ACGGC 1 cut(s) 1331
BciT130I CCWGG 1 cut(s) 620
BclI TGATCA 1 cut(s) 1222
BcnI CCSGG 1 cut(s) 82
BfaI CTAG 6 cut(s) 213, 456, 503, 827, 1136, 1325
BfoI RGCGCY 1 cut(s) 493
BfuAI ACCTGC 1 cut(s) 1113
BisI GCNGC 3 cut(s) 635, 822, 1338
BlsI GCNGC 3 cut(s) 636, 823, 1339
BmcAI AGTACT 2 cut(s) 111, 1235
Bme1390I CCNGG 2 cut(s) 82, 620
BmeT110I CYCGRG 1 cut(s) 368
BmgBI CACGTC 1 cut(s) 1087
BmiI GGNNCC 3 cut(s) 491, 534, 694
BmrFI CCNGG 2 cut(s) 82, 620
BmrI ACTGGG 1 cut(s) 101
BmsI GCATC 5 cut(s) 468, 549, 1172, 1245, 1349
BmuI ACTGGG 1 cut(s) 101
BpiI GAAGAC 4 cut(s) 444, 771, 1087, 1102
BpuMI CCSGG 1 cut(s) 82
BsaHI GRCGYC 1 cut(s) 490
BsaJI CCNNGG 2 cut(s) 619, 726
BsaXI ACNNNNNCTCC 4 cut(s) 244, 274, 963, 993
Bsc4I CCNNNNNNNGG 4 cut(s) 81, 161, 406, 1190
Bse1I ACTGG 3 cut(s) 107, 801, 839
Bse3DI GCAATG 1 cut(s) 491
BseBI CCWGG 1 cut(s) 620
BseDI CCNNGG 2 cut(s) 619, 726
BseGI GGATG 2 cut(s) 871, 1297
BseLI CCNNNNNNNGG 4 cut(s) 81, 161, 406, 1190
BseMI GCAATG 1 cut(s) 491
BseMII CTCAG 2 cut(s) 494, 953
BseNI ACTGG 3 cut(s) 107, 801, 839
BseRI GAGGAG 1 cut(s) 422
BseSI GKGCMC 1 cut(s) 150
BseXI GCAGC 3 cut(s) 621, 808, 1349
BseYI CCCAGC 1 cut(s) 124
BsgI GTGCAG 1 cut(s) 807
Bsh1285I CGRYCG 1 cut(s) 1381
BshNI GGYRCC 2 cut(s) 489, 532
BsiEI CGRYCG 1 cut(s) 1381
BsiHKAI GWGCWC 2 cut(s) 947, 1466
BsiHKCI CYCGRG 1 cut(s) 368
BsiSI CCGG 2 cut(s) 81, 696
BslFI GGGAC 1 cut(s) 382
BslI CCNNNNNNNGG 4 cut(s) 81, 161, 406, 1190
BsmFI GGGAC 1 cut(s) 382
BsoBI CYCGRG 1 cut(s) 368
Bsp1286I GDGCHC 3 cut(s) 150, 947, 1466
Bsp143I GATC 6 cut(s) 94, 215, 680, 698, 780, 1222
Bsp19I CCATGG 1 cut(s) 726
BspACI CCGC 4 cut(s) 131, 159, 913, 1381
BspCNI CTCAG 2 cut(s) 493, 952
BspLI GGNNCC 3 cut(s) 491, 534, 694
BspMI ACCTGC 1 cut(s) 1113
BspPI GGATC 2 cut(s) 688, 706
BspT107I GGYRCC 2 cut(s) 489, 532
BsrDI GCAATG 1 cut(s) 491
BsrI ACTGG 3 cut(s) 107, 801, 839
BssECI CCNNGG 2 cut(s) 619, 726
BssMI GATC 6 cut(s) 94, 215, 680, 698, 780, 1222
BssNAI GTATAC 1 cut(s) 209
BssNI GRCGYC 1 cut(s) 490
BssT1I CCWWGG 1 cut(s) 726
Bst1107I GTATAC 1 cut(s) 209
Bst2UI CCWGG 1 cut(s) 620
Bst4CI ACNGT 8 cut(s) 317, 466, 525, 673, 874, 1103, 1151, 1420
Bst6I CTCTTC 2 cut(s) 23, 620
BstACI GRCGYC 1 cut(s) 490
BstC8I GCNNGC 3 cut(s) 531, 1320, 1374
BstDEI CTNAG 2 cut(s) 480, 939
BstDSI CCRYGG 1 cut(s) 726
BstENI CCTNNNNNAGG 1 cut(s) 1188
BstF5I GGATG 2 cut(s) 871, 1297
BstH2I RGCGCY 1 cut(s) 493
BstHHI GCGC 2 cut(s) 492, 1133
BstKTI GATC 6 cut(s) 97, 218, 683, 701, 783, 1225
BstMBI GATC 6 cut(s) 94, 215, 680, 698, 780, 1222
BstMCI CGRYCG 1 cut(s) 1381
BstMWI GCNNNNNNNGC 6 cut(s) 896, 905, 971, 1128, 1139, 1334
BstNI CCWGG 1 cut(s) 620
BstNSI RCATGY 4 cut(s) 772, 1221, 1322, 1376
BstSCI CCNGG 2 cut(s) 80, 618
BstSLI GKGCMC 1 cut(s) 150
BstV1I GCAGC 3 cut(s) 621, 808, 1349
BstV2I GAAGAC 4 cut(s) 444, 771, 1087, 1102
BstX2I RGATCY 1 cut(s) 698
BstYI RGATCY 1 cut(s) 698
BstZ17I GTATAC 1 cut(s) 209
BtgI CCRYGG 1 cut(s) 726
BtrI CACGTC 1 cut(s) 1087
BtsCI GGATG 2 cut(s) 871, 1297
BtsI GCAGTG 1 cut(s) 513
BtsIMutI CAGTG 4 cut(s) 347, 462, 513, 690
BveI ACCTGC 1 cut(s) 1113
Cac8I GCNNGC 3 cut(s) 531, 1320, 1374
CfoI GCGC 2 cut(s) 492, 1133
Csp6I GTAC 4 cut(s) 110, 430, 1152, 1234
CviAII CATG 8 cut(s) 394, 727, 769, 1025, 1143, 1218, 1319, 1373
CviJI RGCY 6 cut(s) 5, 379, 695, 850, 890, 965
CviKI_1 RGCY 6 cut(s) 5, 379, 695, 850, 890, 965
CviQI GTAC 4 cut(s) 110, 430, 1152, 1234
DdeI CTNAG 2 cut(s) 480, 939
DinI GGCGCC 1 cut(s) 491
DpnI GATC 6 cut(s) 96, 217, 682, 700, 782, 1224
DpnII GATC 6 cut(s) 94, 215, 680, 698, 780, 1222
DrdI GACNNNNNNGTC 1 cut(s) 1104
DseDI GACNNNNNNGTC 1 cut(s) 1104
Eam1104I CTCTTC 2 cut(s) 23, 620
EarI CTCTTC 2 cut(s) 23, 620
Eco130I CCWWGG 1 cut(s) 726
Eco88I CYCGRG 1 cut(s) 368
EcoNI CCTNNNNNAGG 1 cut(s) 1188
EcoRI GAATTC 1 cut(s) 1349
EcoRII CCWGG 1 cut(s) 618
EcoT14I CCWWGG 1 cut(s) 726
EgeI GGCGCC 1 cut(s) 491
EheI GGCGCC 1 cut(s) 491
ErhI CCWWGG 1 cut(s) 726
FaeI CATG 8 cut(s) 397, 730, 772, 1028, 1146, 1221, 1322, 1376
FaqI GGGAC 1 cut(s) 382
FatI CATG 8 cut(s) 393, 726, 768, 1024, 1142, 1217, 1318, 1372
FbaI TGATCA 1 cut(s) 1222
FblI GTMKAC 1 cut(s) 208
Fnu4HI GCNGC 3 cut(s) 635, 822, 1338
FokI GGATG 2 cut(s) 878, 1304
Fsp4HI GCNGC 3 cut(s) 635, 822, 1338
FspBI CTAG 6 cut(s) 213, 456, 503, 827, 1136, 1325
GlaI GCGC 2 cut(s) 491, 1132
GluI GCNGC 3 cut(s) 635, 822, 1338
GsaI CCCAGC 1 cut(s) 128
HaeII RGCGCY 1 cut(s) 493
HapII CCGG 2 cut(s) 81, 696
HhaI GCGC 2 cut(s) 492, 1133
Hin1I GRCGYC 1 cut(s) 490
Hin1II CATG 8 cut(s) 397, 730, 772, 1028, 1146, 1221, 1322, 1376
Hin6I GCGC 2 cut(s) 490, 1131
HinP1I GCGC 2 cut(s) 490, 1131
HincII GTYRAC 1 cut(s) 121
HindII GTYRAC 1 cut(s) 121
HindIII AAGCTT 1 cut(s) 848
HpaII CCGG 2 cut(s) 81, 696
HphI GGTGA 2 cut(s) 305, 1345
Hpy166II GTNNAC 4 cut(s) 121, 188, 209, 1275
Hpy188I TCNGA 6 cut(s) 249, 780, 942, 1177, 1411, 1435
Hpy188III TCNNGA 6 cut(s) 98, 368, 370, 950, 1109, 1346
Hpy8I GTNNAC 4 cut(s) 121, 188, 209, 1275
HpyAV CCTTC 4 cut(s) 20, 620, 1255, 1403
HpyCH4III ACNGT 8 cut(s) 317, 466, 525, 673, 874, 1103, 1151, 1420
HpyCH4IV ACGT 1 cut(s) 1086
HpyCH4V TGCA 7 cut(s) 236, 289, 772, 824, 999, 1122, 1163
HpyF10VI GCNNNNNNNGC 6 cut(s) 896, 905, 971, 1128, 1139, 1334
HpyF3I CTNAG 2 cut(s) 480, 939
HpySE526I ACGT 1 cut(s) 1086
Hsp92I GRCGYC 1 cut(s) 490
Hsp92II CATG 8 cut(s) 397, 730, 772, 1028, 1146, 1221, 1322, 1376
HspAI GCGC 2 cut(s) 490, 1131
KasI GGCGCC 1 cut(s) 489
Ksp22I TGATCA 1 cut(s) 1222
Kzo9I GATC 6 cut(s) 94, 215, 680, 698, 780, 1222
LmnI GCTCC 3 cut(s) 692, 971, 1461
Lsp1109I GCAGC 3 cut(s) 621, 808, 1349
LweI GCATC 5 cut(s) 468, 549, 1172, 1245, 1349
MaeI CTAG 6 cut(s) 213, 456, 503, 827, 1136, 1325
MaeII ACGT 1 cut(s) 1086
MaeIII GTNAC 3 cut(s) 667, 830, 1087
MalI GATC 6 cut(s) 96, 217, 682, 700, 782, 1224
MboI GATC 6 cut(s) 94, 215, 680, 698, 780, 1222
MboII GAAGA 9 cut(s) 10, 444, 482, 607, 649, 657, 776, 1092, 1107
MflI RGATCY 1 cut(s) 698
MhlI GDGCHC 3 cut(s) 150, 947, 1466
Mly113I GGCGCC 1 cut(s) 490
MlyI GAGTC 2 cut(s) 327, 1069
MnlI CCTC 6 cut(s) 24, 276, 295, 400, 837, 1132
MroXI GAANNNNTTC 2 cut(s) 192, 851
MseI TTAA 1 cut(s) 1188
MslI CAYNNNNRTG 4 cut(s) 55, 318, 575, 1013
MspA1I CMGCKG 1 cut(s) 161
MspI CCGG 2 cut(s) 81, 696
MspR9I CCNGG 2 cut(s) 82, 620
MvaI CCWGG 1 cut(s) 620
MwoI GCNNNNNNNGC 6 cut(s) 896, 905, 971, 1128, 1139, 1334
NarI GGCGCC 1 cut(s) 490
NciI CCSGG 1 cut(s) 82
NcoI CCATGG 1 cut(s) 726
NdeII GATC 6 cut(s) 94, 215, 680, 698, 780, 1222
NlaIII CATG 8 cut(s) 397, 730, 772, 1028, 1146, 1221, 1322, 1376
NlaIV GGNNCC 3 cut(s) 491, 534, 694
NmuCI GTSAC 1 cut(s) 1087
NspI RCATGY 4 cut(s) 772, 1221, 1322, 1376
OliI CACNNNNGTG 1 cut(s) 1013
PaeI GCATGC 2 cut(s) 1322, 1376
PaeR7I CTCGAG 1 cut(s) 368
PciI ACATGT 1 cut(s) 1217
PcsI WCGNNNNNNNCGW 1 cut(s) 1374
PdmI GAANNNNTTC 2 cut(s) 192, 851
PfeI GAWTC 9 cut(s) 323, 386, 513, 924, 1036, 1178, 1307, 1430, 1436
PflFI GACNNNGTC 2 cut(s) 336, 1101
PkrI GCNGC 3 cut(s) 636, 823, 1339
PleI GAGTC 2 cut(s) 327, 1068
PluTI GGCGCC 1 cut(s) 493
PpsI GAGTC 2 cut(s) 327, 1068
PscI ACATGT 1 cut(s) 1217
Psp6I CCWGG 1 cut(s) 618
PspFI CCCAGC 1 cut(s) 124
PspGI CCWGG 1 cut(s) 618
PspN4I GGNNCC 3 cut(s) 491, 534, 694
PsuI RGATCY 1 cut(s) 698
PsyI GACNNNGTC 2 cut(s) 336, 1101
RsaI GTAC 4 cut(s) 111, 431, 1153, 1235
RsaNI GTAC 4 cut(s) 110, 430, 1152, 1234
RseI CAYNNNNRTG 4 cut(s) 55, 318, 575, 1013
SaqAI TTAA 1 cut(s) 1188
SatI GCNGC 3 cut(s) 635, 822, 1338
Sau3AI GATC 6 cut(s) 94, 215, 680, 698, 780, 1222
ScaI AGTACT 2 cut(s) 111, 1235
SchI GAGTC 2 cut(s) 327, 1069
ScrFI CCNGG 2 cut(s) 82, 620
SduI GDGCHC 3 cut(s) 150, 947, 1466
SfaNI GCATC 5 cut(s) 468, 549, 1172, 1245, 1349
SfoI GGCGCC 1 cut(s) 491
Sfr274I CTCGAG 1 cut(s) 368
SlaI CTCGAG 1 cut(s) 368
SmiMI CAYNNNNRTG 4 cut(s) 55, 318, 575, 1013
SmlI CTYRAG 1 cut(s) 368
SmoI CTYRAG 1 cut(s) 368
SphI GCATGC 2 cut(s) 1322, 1376
SsiI CCGC 4 cut(s) 131, 159, 913, 1381
SspDI GGCGCC 1 cut(s) 489
SspMI CTAG 6 cut(s) 213, 456, 503, 827, 1136, 1325
StyD4I CCNGG 2 cut(s) 80, 618
StyI CCWWGG 1 cut(s) 726
TaaI ACNGT 8 cut(s) 317, 466, 525, 673, 874, 1103, 1151, 1420
TaiI ACGT 1 cut(s) 1089
TaqI TCGA 5 cut(s) 41, 196, 369, 951, 1368
TatI WGTACW 4 cut(s) 109, 429, 1151, 1233
TfiI GAWTC 9 cut(s) 323, 386, 513, 924, 1036, 1178, 1307, 1430, 1436
Tru1I TTAA 1 cut(s) 1188
Tru9I TTAA 1 cut(s) 1188
TscAI CASTG 4 cut(s) 347, 469, 513, 690
TseFI GTSAC 1 cut(s) 1087
TseI GCWGC 3 cut(s) 634, 821, 1337
Tsp45I GTSAC 1 cut(s) 1087
TspDTI ATGAA 9 cut(s) 369, 505, 545, 641, 701, 844, 922, 1049, 1428
TspGWI ACGGA 1 cut(s) 1481
TspRI CASTG 4 cut(s) 347, 469, 513, 690
Tth111I GACNNNGTC 2 cut(s) 336, 1101
XagI CCTNNNNNAGG 1 cut(s) 1188
XapI RAATTY 6 cut(s) 238, 652, 733, 748, 788, 1349
XceI RCATGY 4 cut(s) 772, 1221, 1322, 1376
XhoI CTCGAG 1 cut(s) 368
XmiI GTMKAC 1 cut(s) 208
XmnI GAANNNNTTC 2 cut(s) 192, 851
XspI CTAG 6 cut(s) 213, 456, 503, 827, 1136, 1325
ZrmI AGTACT 2 cut(s) 111, 1235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.