MD12G1009900.v1.1

NEMP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0004377
Physical Location & Seq
Forward (+)
1014605 .. 1018848
4244 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1009900.v1.1.491

Sequence Viewer

Length: 1491 bp
ATGGGCTCTTCTCTCTTCTCCGCCATTCTCCTTCTTCTCTTTGCTTTCTGTCTCTCTCTTCCGACCCTCTCACCTGCCACCGAACTCAAAGGTGTTGATATTGAAGACCCAGTACTAGAAGTCATCCCGGCACCTCTTTCTGAGCACTCAGCTGTGCCTGGTTCTAAAGATGTTATATTTTGTGAACGGGCACGAGTTTCTGGTATATCCAGATTAAAACTTGGGAGTTATGCAAGTTCACTTAAGATCACCTTGTCTACATCTGTTGCAATCCCGGAGAGGTTGCATAGCAAAATTCAAGTCTGTTTTCATAGGAATAATACGCTTGGATTATGTCAGTGTGAAGAGGATGATTGGAAAAATATTCAGAAAGGGTTATGGAGCTCTGTCTTGTCGCCTTATGATGAAAGATATGTCGATGTCAAGTTCATTGGCAAAATACCTGGCTCTGTCACCATAACTGTTGAAGAAGATTTTCAGAGATGGCGCCTTGTGTGTCTGGCACTGGGATTTACTTTACTGTTGTTGGCACCAATTGTCAGCAATTGGGTACCTTTTTATTATACCAGTTCAATGGTTATAGGAGTTTTTCTGGTCATTATAATCATTCTCTTCCAGGGAATGAAGCTGTTGCCAACTGGAAGGAAAAATGTCCTCTATCTTACCATATATGGATCAGTGCTTGGAGCAGGATCTTTCCTTGTTCATCAGTTTTCACTGATGGTAAATTCAATTCTTTTGAGTTTTGGGCTAAGTGAAGAGATGCATAACCCAGTCTCTATATTTTTACTAGTGGGAATTATCCTCACGGGAGCTGCATTAGGGTACTGGATTGTGAGGAAGTTTGTTGTCTCAAAAGATGGGACAGTAGATGTTGGTATAGCACAATTTGTCAAGTGGGCTATGCGTATCATTGGAACAACCTCCATCTTGCAGAGCACTCTCGATACTCCTTTAGCAATGGGCGCGTTGGTTAATTACTGGATTATCAGCAAGTTGATTACTTCTCTGAAATGGCATCTCAAATCTCATCAGTCGGATGCTGGGAGTGGGAGTCCTTGTCTGCCGAAGGGAAAACAGGTCAAGGGTAGACAAAGTCATCCAAAATTTCTTAGCAGGTCTAGTCCACAAGAAAAGAATTGGAACAGCCGAAGGAGTCTATCTGCTTGGTCTGACTCTCCTGTTACAGGTGTCATGTCCCCATCTTCCAGGGCACCTAACCAACAGGAGTACTATTCAACCTTCCACAAGGTGCAGAACAGAAAGAAGTTCACAAGGAAAGAGTGGGATGATTTCACACATGAATCTACCCGCCAAGCTATAGCAGAATGGGCAGCATCTCCTGAAGTCCCTAATTGGCTCATCGAGCATGCTGATCGCATACAACTCCTTCCAAGCGAGAGTTCAGATGAAACTGGGGGAAGTGAACCAGACTCCATAGATGAGAATGTCGTGGGGAGCACAGATCGAGTAGGTTTTCTTAAGTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NEMP

Protein Analysis

497

Amino Acids

55.07

Weight (kDa)

8.54

Isoelectric Point (pI)

44.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NEMP PF10225 152 - 415 4.7e-48 NEMP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 602
AarI CACCTGC 1 cut(s) 82
Acc36I ACCTGC 2 cut(s) 82, 1107
Acc65I GGTACC 1 cut(s) 550
AccB1I GGYRCC 5 cut(s) 130, 486, 529, 550, 1213
AccI GTMKAC 2 cut(s) 257, 1090
AccII CGCG 1 cut(s) 968
AciI CCGC 2 cut(s) 21, 1312
AclWI GGATC 2 cut(s) 682, 700
AcsI RAATTY 3 cut(s) 294, 727, 1106
AcuI CTGAAG 1 cut(s) 1365
AcyI GRCGYC 1 cut(s) 487
AdeI CACNNNGTG 1 cut(s) 1252
AfaI GTAC 4 cut(s) 114, 552, 827, 1232
AfiI CCNNNNNNNGG 1 cut(s) 1187
AflII CTTAAG 2 cut(s) 242, 1481
AgsI TTSAA 6 cut(s) 104, 299, 467, 573, 732, 1239
AhlI ACTAGT 1 cut(s) 790
AjnI CCWGG 4 cut(s) 157, 442, 615, 1208
AjuI GAANNNNNNNTTGG 2 cut(s) 1387, 1419
AluBI AGCT 5 cut(s) 152, 384, 628, 815, 1319
AluI AGCT 5 cut(s) 152, 384, 628, 815, 1319
Alw21I GWGCWC 4 cut(s) 147, 386, 941, 1463
Alw26I GTCTC 3 cut(s) 56, 781, 856
AlwI GGATC 2 cut(s) 682, 700
ApeKI GCWGC 2 cut(s) 815, 1334
ApoI RAATTY 3 cut(s) 294, 727, 1106
ArsI GACNNNNNNTTYG 2 cut(s) 285, 317
Asp700I GAANNNNTTC 1 cut(s) 474
Asp718I GGTACC 1 cut(s) 550
AspLEI GCGC 2 cut(s) 489, 968
AsuC2I CCSGG 2 cut(s) 128, 275
AsuHPI GGTGA 3 cut(s) 63, 241, 445
BaeGI GKGCMC 2 cut(s) 193, 1216
BanI GGYRCC 5 cut(s) 130, 486, 529, 550, 1213
BanII GRGCYC 2 cut(s) 8, 386
BarI GAAGNNNNNNTAC 2 cut(s) 96, 128
BauI CACGAG 1 cut(s) 192
BbsI GAAGAC 1 cut(s) 111
Bbv12I GWGCWC 4 cut(s) 147, 386, 941, 1463
BbvI GCAGC 2 cut(s) 802, 1346
BccI CCATC 5 cut(s) 477, 715, 854, 935, 1210
BcgI CGANNNNNNTGC 2 cut(s) 1358, 1392
BciT130I CCWGG 4 cut(s) 159, 444, 617, 1210
BcnI CCSGG 2 cut(s) 128, 275
BcoDI GTCTC 3 cut(s) 56, 781, 856
BcuI ACTAGT 1 cut(s) 790
BfaI CTAG 3 cut(s) 116, 791, 1122
BfmI CTRYAG 1 cut(s) 1320
BfoI RGCGCY 1 cut(s) 490
BfrI CTTAAG 2 cut(s) 242, 1481
BfuAI ACCTGC 2 cut(s) 82, 1107
BisI GCNGC 2 cut(s) 816, 1335
BlsI GCNGC 2 cut(s) 817, 1336
BmcAI AGTACT 2 cut(s) 114, 1232
Bme1390I CCNGG 6 cut(s) 128, 159, 275, 444, 617, 1210
BmiI GGNNCC 5 cut(s) 132, 488, 531, 552, 1215
BmrFI CCNGG 6 cut(s) 128, 159, 275, 444, 617, 1210
BmrI ACTGGG 4 cut(s) 104, 515, 767, 1425
BmsI GCATC 4 cut(s) 753, 1027, 1030, 1346
BmuI ACTGGG 4 cut(s) 104, 515, 767, 1425
BpiI GAAGAC 1 cut(s) 111
BpuMI CCSGG 2 cut(s) 128, 275
BsaHI GRCGYC 1 cut(s) 487
BsaJI CCNNGG 2 cut(s) 616, 1209
BsaXI ACNNNNNCTCC 4 cut(s) 1039, 1045, 1069, 1075
Bsc4I CCNNNNNNNGG 1 cut(s) 1187
Bse1I ACTGG 8 cut(s) 110, 510, 567, 643, 773, 833, 986, 1420
Bse3DI GCAATG 1 cut(s) 966
BseBI CCWGG 4 cut(s) 159, 444, 617, 1210
BseDI CCNNGG 2 cut(s) 616, 1209
BseGI GGATG 5 cut(s) 123, 355, 1045, 1099, 1294
BseLI CCNNNNNNNGG 1 cut(s) 1187
BseMI GCAATG 1 cut(s) 966
BseMII CTCAG 2 cut(s) 132, 162
BseNI ACTGG 8 cut(s) 110, 510, 567, 643, 773, 833, 986, 1420
BseSI GKGCMC 2 cut(s) 193, 1216
BseXI GCAGC 2 cut(s) 802, 1346
BseYI CCCAGC 1 cut(s) 1043
BsgI GTGCAG 1 cut(s) 1274
Bsh1236I CGCG 1 cut(s) 968
BshNI GGYRCC 5 cut(s) 130, 486, 529, 550, 1213
BsiHKAI GWGCWC 4 cut(s) 147, 386, 941, 1463
BsiSI CCGG 2 cut(s) 128, 275
BslFI GGGAC 3 cut(s) 877, 1183, 1334
BslI CCNNNNNNNGG 1 cut(s) 1187
BsmAI GTCTC 3 cut(s) 56, 781, 856
BsmFI GGGAC 3 cut(s) 877, 1183, 1334
Bsp1286I GDGCHC 7 cut(s) 8, 147, 193, 386, 941, 1216, 1463
Bsp143I GATC 5 cut(s) 246, 674, 692, 1375, 1465
BspACI CCGC 2 cut(s) 21, 1312
BspCNI CTCAG 2 cut(s) 133, 161
BspFNI CGCG 1 cut(s) 968
BspLI GGNNCC 5 cut(s) 132, 488, 531, 552, 1215
BspMI ACCTGC 2 cut(s) 82, 1107
BspPI GGATC 2 cut(s) 682, 700
BspQI GCTCTTC 1 cut(s) 13
BspT107I GGYRCC 5 cut(s) 130, 486, 529, 550, 1213
BspTI CTTAAG 2 cut(s) 242, 1481
BsrDI GCAATG 1 cut(s) 966
BsrI ACTGG 8 cut(s) 110, 510, 567, 643, 773, 833, 986, 1420
BssECI CCNNGG 2 cut(s) 616, 1209
BssMI GATC 5 cut(s) 246, 674, 692, 1375, 1465
BssNI GRCGYC 1 cut(s) 487
BssSI CACGAG 1 cut(s) 192
Bst2BI CACGAG 1 cut(s) 192
Bst2UI CCWGG 4 cut(s) 159, 444, 617, 1210
Bst4CI ACNGT 3 cut(s) 463, 522, 868
Bst6I CTCTTC 6 cut(s) 13, 20, 63, 339, 617, 753
BstACI GRCGYC 1 cut(s) 487
BstAFI CTTAAG 2 cut(s) 242, 1481
BstC8I GCNNGC 1 cut(s) 1371
BstDEI CTNAG 4 cut(s) 141, 148, 752, 1112
BstENI CCTNNNNNAGG 1 cut(s) 1185
BstF5I GGATG 5 cut(s) 123, 355, 1045, 1099, 1294
BstFNI CGCG 1 cut(s) 968
BstH2I RGCGCY 1 cut(s) 490
BstHHI GCGC 2 cut(s) 489, 968
BstKTI GATC 5 cut(s) 249, 677, 695, 1378, 1468
BstMAI GTCTC 3 cut(s) 56, 781, 856
BstMBI GATC 5 cut(s) 246, 674, 692, 1375, 1465
BstMWI GCNNNNNNNGC 3 cut(s) 965, 1331, 1366
BstNI CCWGG 4 cut(s) 159, 444, 617, 1210
BstNSI RCATGY 1 cut(s) 1373
BstSCI CCNGG 6 cut(s) 126, 157, 273, 442, 615, 1208
BstSFI CTRYAG 1 cut(s) 1320
BstSLI GKGCMC 2 cut(s) 193, 1216
BstUI CGCG 1 cut(s) 968
BstV1I GCAGC 2 cut(s) 802, 1346
BstV2I GAAGAC 1 cut(s) 111
BstX2I RGATCY 1 cut(s) 692
BstXI CCANNNNNNTGG 1 cut(s) 574
BstYI RGATCY 1 cut(s) 692
BtsCI GGATG 5 cut(s) 123, 355, 1045, 1099, 1294
BtsIMutI CAGTG 4 cut(s) 344, 503, 684, 716
BveI ACCTGC 2 cut(s) 82, 1107
Cac8I GCNNGC 1 cut(s) 1371
CfoI GCGC 2 cut(s) 489, 968
Csp6I GTAC 4 cut(s) 113, 551, 826, 1231
CviAII CATG 3 cut(s) 1195, 1301, 1370
CviQI GTAC 4 cut(s) 113, 551, 826, 1231
DdeI CTNAG 4 cut(s) 141, 148, 752, 1112
DinI GGCGCC 1 cut(s) 488
DpnI GATC 5 cut(s) 248, 676, 694, 1377, 1467
DpnII GATC 5 cut(s) 246, 674, 692, 1375, 1465
DraIII CACNNNGTG 1 cut(s) 1252
Eam1104I CTCTTC 6 cut(s) 13, 20, 63, 339, 617, 753
EarI CTCTTC 6 cut(s) 13, 20, 63, 339, 617, 753
EciI GGCGGA 1 cut(s) 10
Ecl136II GAGCTC 1 cut(s) 384
Eco24I GRGCYC 2 cut(s) 8, 386
Eco53kI GAGCTC 1 cut(s) 384
Eco57I CTGAAG 1 cut(s) 1365
EcoICRI GAGCTC 1 cut(s) 384
EcoNI CCTNNNNNAGG 1 cut(s) 1185
EcoRII CCWGG 4 cut(s) 157, 442, 615, 1208
EcoT22I ATGCAT 1 cut(s) 768
EcoT38I GRGCYC 2 cut(s) 8, 386
EgeI GGCGCC 1 cut(s) 488
EheI GGCGCC 1 cut(s) 488
FaeI CATG 3 cut(s) 1198, 1304, 1373
FalI AAGNNNNNCTT 2 cut(s) 236, 268
FaqI GGGAC 3 cut(s) 877, 1183, 1334
FatI CATG 3 cut(s) 1194, 1300, 1369
FauI CCCGC 1 cut(s) 1319
FblI GTMKAC 2 cut(s) 257, 1090
Fnu4HI GCNGC 2 cut(s) 816, 1335
FokI GGATG 5 cut(s) 110, 362, 1052, 1086, 1301
FriOI GRGCYC 2 cut(s) 8, 386
Fsp4HI GCNGC 2 cut(s) 816, 1335
FspBI CTAG 3 cut(s) 116, 791, 1122
GlaI GCGC 2 cut(s) 488, 967
GluI GCNGC 2 cut(s) 816, 1335
GsaI CCCAGC 1 cut(s) 1047
HaeII RGCGCY 1 cut(s) 490
HapII CCGG 2 cut(s) 128, 275
HhaI GCGC 2 cut(s) 489, 968
Hin1I GRCGYC 1 cut(s) 487
Hin1II CATG 3 cut(s) 1198, 1304, 1373
Hin6I GCGC 2 cut(s) 487, 966
HinP1I GCGC 2 cut(s) 487, 966
HinfI GANTC 5 cut(s) 1054, 1156, 1175, 1304, 1433
HpaII CCGG 2 cut(s) 128, 275
HphI GGTGA 3 cut(s) 63, 241, 445
Hpy166II GTNNAC 7 cut(s) 185, 239, 258, 1091, 1127, 1272, 1427
Hpy188I TCNGA 8 cut(s) 63, 142, 369, 480, 1011, 1039, 1174, 1408
Hpy188III TCNNGA 3 cut(s) 210, 944, 1343
Hpy8I GTNNAC 7 cut(s) 185, 239, 258, 1091, 1127, 1272, 1427
HpyAV CCTTC 6 cut(s) 41, 636, 1063, 1146, 1252, 1400
HpyCH4III ACNGT 3 cut(s) 463, 522, 868
HpyCH4V TGCA 7 cut(s) 233, 269, 286, 766, 818, 934, 1255
HpyF10VI GCNNNNNNNGC 3 cut(s) 965, 1331, 1366
HpyF3I CTNAG 4 cut(s) 141, 148, 752, 1112
Hsp92I GRCGYC 1 cut(s) 487
Hsp92II CATG 3 cut(s) 1198, 1304, 1373
HspAI GCGC 2 cut(s) 487, 966
KasI GGCGCC 1 cut(s) 486
KpnI GGTACC 1 cut(s) 554
Kzo9I GATC 5 cut(s) 246, 674, 692, 1375, 1465
LguI GCTCTTC 1 cut(s) 13
LmnI GCTCC 4 cut(s) 381, 686, 812, 1458
Lsp1109I GCAGC 2 cut(s) 802, 1346
LweI GCATC 4 cut(s) 753, 1027, 1030, 1346
MaeI CTAG 3 cut(s) 116, 791, 1122
MaeIII GTNAC 2 cut(s) 451, 1183
MalI GATC 5 cut(s) 248, 676, 694, 1377, 1467
MboI GATC 5 cut(s) 246, 674, 692, 1375, 1465
MfeI CAATTG 2 cut(s) 534, 544
MflI RGATCY 1 cut(s) 692
MhlI GDGCHC 7 cut(s) 8, 147, 193, 386, 941, 1216, 1463
Mly113I GGCGCC 1 cut(s) 487
MlyI GAGTC 4 cut(s) 1063, 1165, 1169, 1427
MmeI TCCRAC 2 cut(s) 86, 1017
MnlI CCTC 8 cut(s) 77, 144, 273, 340, 665, 815, 831, 934
Mph1103I ATGCAT 1 cut(s) 768
MroXI GAANNNNTTC 1 cut(s) 474
MseI TTAA 4 cut(s) 215, 243, 975, 1482
MspA1I CMGCKG 1 cut(s) 152
MspCI CTTAAG 2 cut(s) 242, 1481
MspI CCGG 2 cut(s) 128, 275
MspR9I CCNGG 6 cut(s) 128, 159, 275, 444, 617, 1210
MunI CAATTG 2 cut(s) 534, 544
MvaI CCWGG 4 cut(s) 159, 444, 617, 1210
MvnI CGCG 1 cut(s) 968
MwoI GCNNNNNNNGC 3 cut(s) 965, 1331, 1366
NarI GGCGCC 1 cut(s) 487
NciI CCSGG 2 cut(s) 128, 275
NdeII GATC 5 cut(s) 246, 674, 692, 1375, 1465
NlaIII CATG 3 cut(s) 1198, 1304, 1373
NlaIV GGNNCC 5 cut(s) 132, 488, 531, 552, 1215
NmuCI GTSAC 1 cut(s) 451
NsiI ATGCAT 1 cut(s) 768
NspI RCATGY 1 cut(s) 1373
PaeI GCATGC 1 cut(s) 1373
PaqCI CACCTGC 1 cut(s) 82
PciSI GCTCTTC 1 cut(s) 13
PdmI GAANNNNTTC 1 cut(s) 474
PfeI GAWTC 1 cut(s) 1304
PflFI GACNNNGTC 1 cut(s) 1095
PfoI TCCNGGA 1 cut(s) 273
PkrI GCNGC 2 cut(s) 817, 1336
PleI GAGTC 4 cut(s) 1062, 1164, 1169, 1427
PluTI GGCGCC 1 cut(s) 490
PpsI GAGTC 4 cut(s) 1062, 1164, 1169, 1427
PsiI TTATAA 1 cut(s) 602
Psp124BI GAGCTC 1 cut(s) 386
Psp6I CCWGG 4 cut(s) 157, 442, 615, 1208
PspFI CCCAGC 1 cut(s) 1043
PspGI CCWGG 4 cut(s) 157, 442, 615, 1208
PspN4I GGNNCC 5 cut(s) 132, 488, 531, 552, 1215
PsuI RGATCY 1 cut(s) 692
PsyI GACNNNGTC 1 cut(s) 1095
PvuII CAGCTG 1 cut(s) 152
RsaI GTAC 4 cut(s) 114, 552, 827, 1232
RsaNI GTAC 4 cut(s) 113, 551, 826, 1231
SacI GAGCTC 1 cut(s) 386
SapI GCTCTTC 1 cut(s) 13
SaqAI TTAA 4 cut(s) 215, 243, 975, 1482
SatI GCNGC 2 cut(s) 816, 1335
Sau3AI GATC 5 cut(s) 246, 674, 692, 1375, 1465
ScaI AGTACT 2 cut(s) 114, 1232
SchI GAGTC 4 cut(s) 1063, 1165, 1169, 1427
ScrFI CCNGG 6 cut(s) 128, 159, 275, 444, 617, 1210
SduI GDGCHC 7 cut(s) 8, 147, 193, 386, 941, 1216, 1463
SfaNI GCATC 4 cut(s) 753, 1027, 1030, 1346
SfcI CTRYAG 1 cut(s) 1320
SfoI GGCGCC 1 cut(s) 488
SmlI CTYRAG 2 cut(s) 242, 1481
SmoI CTYRAG 2 cut(s) 242, 1481
SpeI ACTAGT 1 cut(s) 790
SphI GCATGC 1 cut(s) 1373
SsiI CCGC 2 cut(s) 21, 1312
SspDI GGCGCC 1 cut(s) 486
SspI AATATT 1 cut(s) 364
SspMI CTAG 3 cut(s) 116, 791, 1122
SstI GAGCTC 1 cut(s) 386
StyD4I CCNGG 6 cut(s) 126, 157, 273, 442, 615, 1208
TaaI ACNGT 3 cut(s) 463, 522, 868
TaqI TCGA 4 cut(s) 417, 945, 1365, 1468
TatI WGTACW 2 cut(s) 112, 1230
TfiI GAWTC 1 cut(s) 1304
Tru1I TTAA 4 cut(s) 215, 243, 975, 1482
Tru9I TTAA 4 cut(s) 215, 243, 975, 1482
TscAI CASTG 4 cut(s) 344, 510, 684, 723
TseFI GTSAC 1 cut(s) 451
TseI GCWGC 2 cut(s) 815, 1334
Tsp45I GTSAC 1 cut(s) 451
TspDTI ATGAA 7 cut(s) 299, 418, 420, 638, 695, 1317, 1425
TspRI CASTG 4 cut(s) 344, 510, 684, 723
Tth111I GACNNNGTC 1 cut(s) 1095
Vha464I CTTAAG 2 cut(s) 242, 1481
XagI CCTNNNNNAGG 1 cut(s) 1185
XapI RAATTY 3 cut(s) 294, 727, 1106
XceI RCATGY 1 cut(s) 1373
XmiI GTMKAC 2 cut(s) 257, 1090
XmnI GAANNNNTTC 1 cut(s) 474
XspI CTAG 3 cut(s) 116, 791, 1122
ZrmI AGTACT 2 cut(s) 114, 1232
Zsp2I ATGCAT 1 cut(s) 768
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.