Rorug03G0280700

NEMP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0004377
Physical Location & Seq
Forward (+)
27882797 .. 27885951
3155 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0280700.1

Sequence Viewer

Length: 558 bp
ATGAAGATCGCCGACGTCAAGGACTGGACGGAGGTCGGAGCTCTGACGGAGGCGTTGAAGGACCAGAAGGTTCTGATACGCGGCCGCACGCAGACGATCCGAGCCTTGAGAAAGGTGGCGTTTATTGTGGTCAGAAAGAAGGGGTTCACGGTTACCCGAAGGAAGAATAAAACGAGACAGGAAGCCCTCATTATTATTATAATTTGGACAATCATATCCATCTGTTCAAGGGAAGTAGCGCAAGGTGGAGAGATTCCTCCTGAGATCGGAAAGATGAGCAGCTTGGAAGTTTTTGTTGGATCTTTTGGATTGCTTGCTTTTGGTTCAGACAGTTTTGTGATTTGTACTGTAATCGGTTTTCCAGGTTTCATGCTTTGTGCAGGATCACAACTTCTGGACCTTCATAGTGGAAACTGGACTTTGGAACATGCAAAGCTCAAAGCTAGGGTGTTTTACAGAGAAATCAAAGTCATTTTATTGGGGGAGGACCTCCTATCGTTAAGTATGAAAGAGCTTCAGAATTTGGAGCAGCAACTTGATTCTGCTCTAAAACACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NEMP

Protein Analysis

185

Amino Acids

20.7

Weight (kDa)

9.48

Isoelectric Point (pI)

30.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
K-box PF01486 136 - 185 1.4e-07 K-box region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 200
AatII GACGTC 1 cut(s) 18
AccII CGCG 1 cut(s) 81
AciI CCGC 2 cut(s) 81, 85
AclWI GGATC 3 cut(s) 91, 307, 391
AcoI YGGCCR 1 cut(s) 82
AcsI RAATTY 1 cut(s) 520
AcuI CTGAAG 1 cut(s) 500
AcyI GRCGYC 1 cut(s) 15
AfaI GTAC 1 cut(s) 346
AfiI CCNNNNNNNGG 1 cut(s) 266
AgsI TTSAA 2 cut(s) 58, 228
AjnI CCWGG 1 cut(s) 361
AjuI GAANNNNNNNTTGG 2 cut(s) 279, 311
AluBI AGCT 5 cut(s) 41, 282, 436, 443, 514
AluI AGCT 5 cut(s) 41, 282, 436, 443, 514
Alw21I GWGCWC 1 cut(s) 43
Alw26I GTCTC 1 cut(s) 169
AlwI GGATC 3 cut(s) 91, 307, 391
AoxI GGCC 1 cut(s) 82
ApeKI GCWGC 2 cut(s) 279, 529
ApoI RAATTY 1 cut(s) 520
Asp700I GAANNNNTTC 1 cut(s) 143
AspLEI GCGC 1 cut(s) 241
AspS9I GGNCC 3 cut(s) 61, 397, 487
AvaII GGWCC 3 cut(s) 61, 397, 487
BanII GRGCYC 1 cut(s) 43
Bbv12I GWGCWC 1 cut(s) 43
BbvI GCAGC 2 cut(s) 291, 541
BccI CCATC 1 cut(s) 227
BciT130I CCWGG 1 cut(s) 363
BcoDI GTCTC 1 cut(s) 169
BfaI CTAG 2 cut(s) 444, 556
BisI GCNGC 4 cut(s) 82, 85, 280, 530
BlsI GCNGC 4 cut(s) 83, 86, 281, 531
Bme1390I CCNGG 1 cut(s) 363
Bme18I GGWCC 3 cut(s) 61, 397, 487
BmgT120I GGNCC 3 cut(s) 61, 397, 487
BmrFI CCNGG 1 cut(s) 363
BoxI GACNNNNGTC 1 cut(s) 32
BpuEI CTTGAG 1 cut(s) 127
BsaHI GRCGYC 1 cut(s) 15
Bsc4I CCNNNNNNNGG 1 cut(s) 266
Bse1I ACTGG 2 cut(s) 29, 419
BseBI CCWGG 1 cut(s) 363
BseLI CCNNNNNNNGG 1 cut(s) 266
BseMII CTCAG 1 cut(s) 252
BseNI ACTGG 2 cut(s) 29, 419
BseX3I CGGCCG 1 cut(s) 82
BseXI GCAGC 2 cut(s) 291, 541
BsgI GTGCAG 1 cut(s) 399
Bsh1236I CGCG 1 cut(s) 81
Bsh1285I CGRYCG 1 cut(s) 85
BshFI GGCC 1 cut(s) 84
BsiEI CGRYCG 1 cut(s) 85
BsiHKAI GWGCWC 1 cut(s) 43
BslI CCNNNNNNNGG 1 cut(s) 266
BsmAI GTCTC 1 cut(s) 169
BsnI GGCC 1 cut(s) 84
Bsp1286I GDGCHC 1 cut(s) 43
Bsp143I GATC 5 cut(s) 6, 96, 264, 299, 383
BspACI CCGC 2 cut(s) 81, 85
BspANI GGCC 1 cut(s) 84
BspCNI CTCAG 1 cut(s) 253
BspFNI CGCG 1 cut(s) 81
BspPI GGATC 3 cut(s) 91, 307, 391
BsrI ACTGG 2 cut(s) 29, 419
BssMI GATC 5 cut(s) 6, 96, 264, 299, 383
BssNI GRCGYC 1 cut(s) 15
Bst2UI CCWGG 1 cut(s) 363
Bst4CI ACNGT 3 cut(s) 151, 332, 349
BstACI GRCGYC 1 cut(s) 15
BstC8I GCNNGC 2 cut(s) 89, 315
BstDEI CTNAG 1 cut(s) 261
BstEII GGTNACC 1 cut(s) 151
BstFNI CGCG 1 cut(s) 81
BstHHI GCGC 1 cut(s) 241
BstKTI GATC 5 cut(s) 9, 99, 267, 302, 386
BstMAI GTCTC 1 cut(s) 169
BstMBI GATC 5 cut(s) 6, 96, 264, 299, 383
BstMCI CGRYCG 1 cut(s) 85
BstNI CCWGG 1 cut(s) 363
BstNSI RCATGY 1 cut(s) 431
BstPAI GACNNNNGTC 1 cut(s) 32
BstPI GGTNACC 1 cut(s) 151
BstSCI CCNGG 1 cut(s) 361
BstUI CGCG 1 cut(s) 81
BstV1I GCAGC 2 cut(s) 291, 541
BstX2I RGATCY 1 cut(s) 299
BstYI RGATCY 1 cut(s) 299
BstZI CGGCCG 1 cut(s) 82
BsuRI GGCC 1 cut(s) 84
Cac8I GCNNGC 2 cut(s) 89, 315
CciNI GCGGCCGC 1 cut(s) 82
CfoI GCGC 1 cut(s) 241
Cfr13I GGNCC 3 cut(s) 61, 397, 487
Csp6I GTAC 1 cut(s) 345
CviAII CATG 2 cut(s) 370, 428
CviJI RGCY 8 cut(s) 41, 84, 104, 185, 282, 436, 443, 514
CviKI_1 RGCY 8 cut(s) 41, 84, 104, 185, 282, 436, 443, 514
CviQI GTAC 1 cut(s) 345
DdeI CTNAG 1 cut(s) 261
DpnI GATC 5 cut(s) 8, 98, 266, 301, 385
DpnII GATC 5 cut(s) 6, 96, 264, 299, 383
EaeI YGGCCR 1 cut(s) 82
EagI CGGCCG 1 cut(s) 82
Ecl136II GAGCTC 1 cut(s) 41
EclXI CGGCCG 1 cut(s) 82
Eco24I GRGCYC 1 cut(s) 43
Eco47I GGWCC 3 cut(s) 61, 397, 487
Eco52I CGGCCG 1 cut(s) 82
Eco53kI GAGCTC 1 cut(s) 41
Eco57I CTGAAG 1 cut(s) 500
Eco91I GGTNACC 1 cut(s) 151
EcoICRI GAGCTC 1 cut(s) 41
EcoO109I RGGNCCY 1 cut(s) 487
EcoO65I GGTNACC 1 cut(s) 151
EcoRII CCWGG 1 cut(s) 361
EcoT38I GRGCYC 1 cut(s) 43
FaeI CATG 2 cut(s) 373, 431
FaiI YATR 6 cut(s) 200, 215, 371, 405, 429, 506
FatI CATG 2 cut(s) 369, 427
Fnu4HI GCNGC 4 cut(s) 82, 85, 280, 530
FriOI GRGCYC 1 cut(s) 43
Fsp4HI GCNGC 4 cut(s) 82, 85, 280, 530
FspBI CTAG 2 cut(s) 444, 556
GlaI GCGC 1 cut(s) 240
GluI GCNGC 4 cut(s) 82, 85, 280, 530
HaeIII GGCC 1 cut(s) 84
HhaI GCGC 1 cut(s) 241
Hin1I GRCGYC 1 cut(s) 15
Hin1II CATG 2 cut(s) 373, 431
Hin6I GCGC 1 cut(s) 239
HinP1I GCGC 1 cut(s) 239
HinfI GANTC 2 cut(s) 253, 539
Hpy166II GTNNAC 1 cut(s) 147
Hpy188I TCNGA 8 cut(s) 38, 45, 75, 101, 134, 269, 328, 519
Hpy188III TCNNGA 2 cut(s) 260, 395
Hpy8I GTNNAC 1 cut(s) 147
Hpy99I CGWCG 1 cut(s) 17
HpyAV CCTTC 5 cut(s) 52, 61, 133, 153, 410
HpyCH4III ACNGT 3 cut(s) 151, 332, 349
HpyCH4IV ACGT 1 cut(s) 15
HpyCH4V TGCA 2 cut(s) 380, 431
HpyF3I CTNAG 1 cut(s) 261
HpySE526I ACGT 1 cut(s) 15
Hsp92I GRCGYC 1 cut(s) 15
Hsp92II CATG 2 cut(s) 373, 431
HspAI GCGC 1 cut(s) 239
Kzo9I GATC 5 cut(s) 6, 96, 264, 299, 383
LmnI GCTCC 2 cut(s) 38, 526
LpnPI CCDG 9 cut(s) 10, 77, 164, 273, 348, 366, 375, 380, 400
Lsp1109I GCAGC 2 cut(s) 291, 541
MaeI CTAG 2 cut(s) 444, 556
MaeII ACGT 1 cut(s) 15
MaeIII GTNAC 1 cut(s) 151
MalI GATC 5 cut(s) 8, 98, 266, 301, 385
MboI GATC 5 cut(s) 6, 96, 264, 299, 383
MboII GAAGA 2 cut(s) 16, 175
MflI RGATCY 1 cut(s) 299
MhlI GDGCHC 1 cut(s) 43
MluCI AATT 2 cut(s) 201, 520
MmeI TCCRAC 2 cut(s) 16, 277
MnlI CCTC 6 cut(s) 25, 43, 197, 267, 478, 500
MroXI GAANNNNTTC 1 cut(s) 143
MseI TTAA 1 cut(s) 500
MspR9I CCNGG 1 cut(s) 363
MvaI CCWGG 1 cut(s) 363
MvnI CGCG 1 cut(s) 81
NdeII GATC 5 cut(s) 6, 96, 264, 299, 383
NlaIII CATG 2 cut(s) 373, 431
NotI GCGGCCGC 1 cut(s) 82
NspI RCATGY 1 cut(s) 431
PdmI GAANNNNTTC 1 cut(s) 143
PfeI GAWTC 2 cut(s) 253, 539
PkrI GCNGC 4 cut(s) 83, 86, 281, 531
PpuMI RGGWCCY 1 cut(s) 487
PshAI GACNNNNGTC 1 cut(s) 32
PsiI TTATAA 1 cut(s) 200
Psp124BI GAGCTC 1 cut(s) 43
Psp5II RGGWCCY 1 cut(s) 487
Psp6I CCWGG 1 cut(s) 361
PspEI GGTNACC 1 cut(s) 151
PspGI CCWGG 1 cut(s) 361
PspPI GGNCC 3 cut(s) 61, 397, 487
PspPPI RGGWCCY 1 cut(s) 487
PsuI RGATCY 1 cut(s) 299
RsaI GTAC 1 cut(s) 346
RsaNI GTAC 1 cut(s) 345
SacI GAGCTC 1 cut(s) 43
SaqAI TTAA 1 cut(s) 500
SatI GCNGC 4 cut(s) 82, 85, 280, 530
Sau3AI GATC 5 cut(s) 6, 96, 264, 299, 383
Sau96I GGNCC 3 cut(s) 61, 397, 487
ScrFI CCNGG 1 cut(s) 363
SduI GDGCHC 1 cut(s) 43
SinI GGWCC 3 cut(s) 61, 397, 487
SmlI CTYRAG 1 cut(s) 106
SmoI CTYRAG 1 cut(s) 106
Sse9I AATT 2 cut(s) 201, 520
SsiI CCGC 2 cut(s) 81, 85
SspMI CTAG 2 cut(s) 444, 556
SstI GAGCTC 1 cut(s) 43
StyD4I CCNGG 1 cut(s) 361
TaaI ACNGT 3 cut(s) 151, 332, 349
TaiI ACGT 1 cut(s) 18
TasI AATT 2 cut(s) 201, 520
TatI WGTACW 1 cut(s) 344
TauI GCSGC 2 cut(s) 84, 87
TfiI GAWTC 2 cut(s) 253, 539
Tru1I TTAA 1 cut(s) 500
Tru9I TTAA 1 cut(s) 500
TseI GCWGC 2 cut(s) 279, 529
TspDTI ATGAA 4 cut(s) 17, 358, 392, 521
TspGWI ACGGA 2 cut(s) 44, 62
VpaK11BI GGWCC 3 cut(s) 61, 397, 487
XapI RAATTY 1 cut(s) 520
XceI RCATGY 1 cut(s) 431
XmnI GAANNNNTTC 1 cut(s) 143
XspI CTAG 2 cut(s) 444, 556
ZraI GACGTC 1 cut(s) 16
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.