AT1G52315

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003526
Physical Location & Seq
Forward (+)
19481046 .. 19482863
1818 bp
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UTR
Exon/CDS
Intron
AT1G52315.1

Sequence Viewer

Length: 1044 bp
ATGTTTCTTAGGTGCTTCAAACCCAAGTTCTACAAGAAAAGCAAATCTACAACCAGCTACATGAAGATTAGGATTGATATAGTTAGGAGAAAAAGGATTGCAATGGTTAGAAATTATAAAACAGATATTGTGAATTTCCTCAAGAATGGCCAAGATTCTGAAGCTTATAGAAGAGCAGAACTTTTACTCGAAGAGCTGAGAATAATATCGTGCTACGATCTCATCGAGCGATTCTGCGATTGTATCTCTGAAAATCTCTCACTAATGCTGAAGAAAAGGGAATGTCCTGAAGAATGCAGAGAAGCTGTTTCCTCTTTGATATATGCAACGGCATGGGTTCCTGATGTTCCTGAACTTAAGGATCTTAGAGCCGTGTTTACGAAAAGATTTGGGAATTTCATTGCTTCATCTGTAAATCATGAGCTTGTTGAGAAAACTGAATTGTTAAGGCCACCTTCACGGGAACTCAAGATTCAAACAGTGAAAGATGTTGCGAATGAGTTTTCGATTAACTGGGATCCTACACCTTTGAAACTGATGCTGCTCAGAGAGACTTCAGCTCTGCAGATTAAGGACAAGGTCGAGACAAGAGCGGATTATCTGAACAAAGATACAGAGAAAAGTTTCATGGATGAACATAGTGATGATGAATCGGTTCTTTCTGAGAGTTGGAGAATAGATTCATTGTCCAAAGGCAGTTTGAGTTCTAGTTCAAGCTCGAGCTCGAGTTCTCTTCGAAGGGTATCTGTAAGGAAGAAGAAGGGGAAGAAGATTTTACCGTACGGAATAATCTCTTCTCCAGACACGAAACTAGGAGCTGGAAACGACGAGAAAGCACAAGAGGAGAAAGAAGAGAAGAGAAAGATTGCTGATCAAGAAAACTCAAGGTTACTTGGAATACAAGGTAGTAGGAGTCTCACAAGTACAAGCAATAACGAAGTTTCCACAATAAGAGAGAACGAGAGAATGTCGTCGTTTCAAAGACCTAACCTCCTTGATTATGATGATGTGGTGGCTCGTCTTGCAGCTCTGCGCCGGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

347

Amino Acids

40.0

Weight (kDa)

9.24

Isoelectric Point (pI)

55.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 20 - 171 1.8e-39 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 117
AccBSI CCGCTC 1 cut(s) 593
AciI CCGC 1 cut(s) 593
AclWI GGATC 3 cut(s) 369, 512, 525
AcoI YGGCCR 1 cut(s) 148
AcsI RAATTY 2 cut(s) 133, 394
AcuI CTGAAG 4 cut(s) 180, 290, 309, 540
AfaI GTAC 2 cut(s) 782, 925
AflII CTTAAG 1 cut(s) 356
AgsI TTSAA 5 cut(s) 19, 476, 532, 714, 980
Alw21I GWGCWC 1 cut(s) 725
Alw26I GTCTC 3 cut(s) 545, 578, 920
AlwI GGATC 3 cut(s) 369, 512, 525
Ama87I CYCGRG 2 cut(s) 718, 724
AoxI GGCC 2 cut(s) 148, 449
ApeKI GCWGC 2 cut(s) 541, 1025
ApoI RAATTY 2 cut(s) 133, 394
Asp700I GAANNNNTTC 3 cut(s) 623, 654, 679
AspLEI GCGC 1 cut(s) 1035
AsuII TTCGAA 1 cut(s) 736
AvaI CYCGRG 2 cut(s) 718, 724
BalI TGGCCA 1 cut(s) 150
BamHI GGATCC 1 cut(s) 517
BanII GRGCYC 1 cut(s) 725
Bbv12I GWGCWC 1 cut(s) 725
BbvI GCAGC 2 cut(s) 528, 1037
BceAI ACGGC 2 cut(s) 345, 356
BclI TGATCA 1 cut(s) 871
BcoDI GTCTC 3 cut(s) 545, 578, 920
BfaI CTAG 2 cut(s) 708, 812
BfmI CTRYAG 1 cut(s) 563
BfrI CTTAAG 1 cut(s) 356
BisI GCNGC 2 cut(s) 542, 1026
BlsI GCNGC 2 cut(s) 543, 1027
BmeT110I CYCGRG 2 cut(s) 718, 724
BmiI GGNNCC 2 cut(s) 339, 519
BmrI ACTGGG 1 cut(s) 523
BmsI GCATC 1 cut(s) 528
BmuI ACTGGG 1 cut(s) 523
BpmI CTGGAG 1 cut(s) 783
Bpu14I TTCGAA 1 cut(s) 736
BpuEI CTTGAG 3 cut(s) 125, 452, 868
BsaXI ACNNNNNCTCC 2 cut(s) 975, 1005
Bse1I ACTGG 1 cut(s) 518
Bse3DI GCAATG 2 cut(s) 108, 399
BseGI GGATG 1 cut(s) 637
BseMI GCAATG 2 cut(s) 108, 399
BseMII CTCAG 3 cut(s) 188, 559, 654
BseNI ACTGG 1 cut(s) 518
BseRI GAGGAG 1 cut(s) 857
BseXI GCAGC 2 cut(s) 528, 1037
BshFI GGCC 2 cut(s) 150, 451
BsiHKAI GWGCWC 1 cut(s) 725
BsiHKCI CYCGRG 2 cut(s) 718, 724
BsiSI CCGG 1 cut(s) 1036
BsiWI CGTACG 1 cut(s) 780
BsmAI GTCTC 3 cut(s) 545, 578, 920
BsmI GAATGC 1 cut(s) 299
BsnI GGCC 2 cut(s) 150, 451
BsoBI CYCGRG 2 cut(s) 718, 724
Bsp119I TTCGAA 1 cut(s) 736
Bsp1286I GDGCHC 1 cut(s) 725
Bsp143I GATC 4 cut(s) 217, 361, 517, 871
BspACI CCGC 1 cut(s) 593
BspANI GGCC 2 cut(s) 150, 451
BspCNI CTCAG 3 cut(s) 189, 558, 655
BspHI TCATGA 1 cut(s) 418
BspLI GGNNCC 2 cut(s) 339, 519
BspMAI CTGCAG 1 cut(s) 567
BspPI GGATC 3 cut(s) 369, 512, 525
BspQI GCTCTTC 2 cut(s) 166, 186
BspT104I TTCGAA 1 cut(s) 736
BspTI CTTAAG 1 cut(s) 356
BsrBI CCGCTC 1 cut(s) 593
BsrDI GCAATG 2 cut(s) 108, 399
BsrI ACTGG 1 cut(s) 518
BssMI GATC 4 cut(s) 217, 361, 517, 871
Bst4CI ACNGT 2 cut(s) 481, 780
Bst6I CTCTTC 6 cut(s) 166, 186, 738, 799, 846, 851
BstAFI CTTAAG 1 cut(s) 356
BstBI TTCGAA 1 cut(s) 736
BstDEI CTNAG 5 cut(s) 8, 197, 365, 545, 663
BstF5I GGATG 1 cut(s) 637
BstHHI GCGC 1 cut(s) 1035
BstKTI GATC 4 cut(s) 220, 364, 520, 874
BstMAI GTCTC 3 cut(s) 545, 578, 920
BstMBI GATC 4 cut(s) 217, 361, 517, 871
BstMWI GCNNNNNNNGC 1 cut(s) 1022
BstSFI CTRYAG 1 cut(s) 563
BstV1I GCAGC 2 cut(s) 528, 1037
BstX2I RGATCY 2 cut(s) 361, 517
BstYI RGATCY 2 cut(s) 361, 517
BsuRI GGCC 2 cut(s) 150, 451
BtsCI GGATG 1 cut(s) 637
BtsIMutI CAGTG 1 cut(s) 486
CciI TCATGA 1 cut(s) 418
CfoI GCGC 1 cut(s) 1035
Csp6I GTAC 2 cut(s) 781, 924
CviAII CATG 4 cut(s) 61, 333, 419, 628
CviQI GTAC 2 cut(s) 781, 924
DdeI CTNAG 5 cut(s) 8, 197, 365, 545, 663
DpnI GATC 4 cut(s) 219, 363, 519, 873
DpnII GATC 4 cut(s) 217, 361, 517, 871
EaeI YGGCCR 1 cut(s) 148
Eam1104I CTCTTC 6 cut(s) 166, 186, 738, 799, 846, 851
EarI CTCTTC 6 cut(s) 166, 186, 738, 799, 846, 851
Ecl136II GAGCTC 1 cut(s) 723
Eco24I GRGCYC 1 cut(s) 725
Eco53kI GAGCTC 1 cut(s) 723
Eco57I CTGAAG 4 cut(s) 180, 290, 309, 540
Eco88I CYCGRG 2 cut(s) 718, 724
EcoICRI GAGCTC 1 cut(s) 723
EcoT38I GRGCYC 1 cut(s) 725
FaeI CATG 4 cut(s) 64, 336, 422, 631
FalI AAGNNNNNCTT 2 cut(s) 439, 471
FatI CATG 4 cut(s) 60, 332, 418, 627
FbaI TGATCA 1 cut(s) 871
Fnu4HI GCNGC 2 cut(s) 542, 1026
FokI GGATG 1 cut(s) 644
FriOI GRGCYC 1 cut(s) 725
Fsp4HI GCNGC 2 cut(s) 542, 1026
FspBI CTAG 2 cut(s) 708, 812
GlaI GCGC 1 cut(s) 1034
GluI GCNGC 2 cut(s) 542, 1026
GsuI CTGGAG 1 cut(s) 783
HaeIII GGCC 2 cut(s) 150, 451
HapII CCGG 1 cut(s) 1036
HhaI GCGC 1 cut(s) 1035
Hin1II CATG 4 cut(s) 64, 336, 422, 631
Hin6I GCGC 1 cut(s) 1033
HinP1I GCGC 1 cut(s) 1033
HindIII AAGCTT 1 cut(s) 162
HinfI GANTC 6 cut(s) 155, 231, 472, 650, 680, 913
HpaII CCGG 1 cut(s) 1036
Hpy166II GTNNAC 1 cut(s) 378
Hpy188I TCNGA 5 cut(s) 160, 250, 548, 603, 664
Hpy188III TCNNGA 9 cut(s) 142, 287, 341, 350, 419, 469, 583, 800, 875
Hpy8I GTNNAC 1 cut(s) 378
Hpy99I CGWCG 2 cut(s) 830, 976
HpyAV CCTTC 3 cut(s) 465, 732, 754
HpyCH4III ACNGT 2 cut(s) 481, 780
HpyCH4V TGCA 5 cut(s) 101, 297, 326, 565, 1025
HpyF10VI GCNNNNNNNGC 1 cut(s) 1022
HpyF3I CTNAG 5 cut(s) 8, 197, 365, 545, 663
Hsp92II CATG 4 cut(s) 64, 336, 422, 631
HspAI GCGC 1 cut(s) 1033
Ksp22I TGATCA 1 cut(s) 871
Kzo9I GATC 4 cut(s) 217, 361, 517, 871
LguI GCTCTTC 2 cut(s) 166, 186
LmnI GCTCC 1 cut(s) 815
LpnPI CCDG 7 cut(s) 67, 300, 354, 363, 499, 804, 813
Lsp1109I GCAGC 2 cut(s) 528, 1037
LweI GCATC 1 cut(s) 528
MaeI CTAG 2 cut(s) 708, 812
MaeIII GTNAC 1 cut(s) 888
MalI GATC 4 cut(s) 219, 363, 519, 873
MbiI CCGCTC 1 cut(s) 593
MboI GATC 4 cut(s) 217, 361, 517, 871
MflI RGATCY 2 cut(s) 361, 517
MhlI GDGCHC 1 cut(s) 725
MlsI TGGCCA 1 cut(s) 150
MluCI AATT 4 cut(s) 112, 133, 394, 440
MluNI TGGCCA 1 cut(s) 150
MlyI GAGTC 1 cut(s) 922
MmeI TCCRAC 1 cut(s) 650
MnlI CCTC 4 cut(s) 149, 322, 835, 1001
Mox20I TGGCCA 1 cut(s) 150
MroXI GAANNNNTTC 3 cut(s) 623, 654, 679
MscI TGGCCA 1 cut(s) 150
MseI TTAA 4 cut(s) 357, 446, 510, 570
MslI CAYNNNNRTG 1 cut(s) 642
Msp20I TGGCCA 1 cut(s) 150
MspCI CTTAAG 1 cut(s) 356
MspI CCGG 1 cut(s) 1036
Mva1269I GAATGC 1 cut(s) 299
MwoI GCNNNNNNNGC 1 cut(s) 1022
NdeII GATC 4 cut(s) 217, 361, 517, 871
NlaIII CATG 4 cut(s) 64, 336, 422, 631
NlaIV GGNNCC 2 cut(s) 339, 519
NspV TTCGAA 1 cut(s) 736
PaeR7I CTCGAG 2 cut(s) 718, 724
PagI TCATGA 1 cut(s) 418
PciSI GCTCTTC 2 cut(s) 166, 186
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PctI GAATGC 1 cut(s) 299
PdmI GAANNNNTTC 3 cut(s) 623, 654, 679
PfeI GAWTC 5 cut(s) 155, 231, 472, 650, 680
Pfl23II CGTACG 1 cut(s) 780
PflFI GACNNNGTC 1 cut(s) 578
PkrI GCNGC 2 cut(s) 543, 1027
PleI GAGTC 1 cut(s) 921
PpsI GAGTC 1 cut(s) 921
PsiI TTATAA 1 cut(s) 117
Psp124BI GAGCTC 1 cut(s) 725
PspLI CGTACG 1 cut(s) 780
PspN4I GGNNCC 2 cut(s) 339, 519
PspXI VCTCGAGB 2 cut(s) 718, 724
PstI CTGCAG 1 cut(s) 567
PsuI RGATCY 2 cut(s) 361, 517
PsyI GACNNNGTC 1 cut(s) 578
RsaI GTAC 2 cut(s) 782, 925
RsaNI GTAC 2 cut(s) 781, 924
RseI CAYNNNNRTG 1 cut(s) 642
SacI GAGCTC 1 cut(s) 725
SapI GCTCTTC 2 cut(s) 166, 186
SaqAI TTAA 4 cut(s) 357, 446, 510, 570
SatI GCNGC 2 cut(s) 542, 1026
Sau3AI GATC 4 cut(s) 217, 361, 517, 871
SchI GAGTC 1 cut(s) 922
SduI GDGCHC 1 cut(s) 725
SfaNI GCATC 1 cut(s) 528
SfcI CTRYAG 1 cut(s) 563
Sfr274I CTCGAG 2 cut(s) 718, 724
SfuI TTCGAA 1 cut(s) 736
SlaI CTCGAG 2 cut(s) 718, 724
SmiMI CAYNNNNRTG 1 cut(s) 642
SmlI CTYRAG 6 cut(s) 140, 356, 467, 718, 724, 883
SmoI CTYRAG 6 cut(s) 140, 356, 467, 718, 724, 883
Sse9I AATT 4 cut(s) 112, 133, 394, 440
SsiI CCGC 1 cut(s) 593
SspMI CTAG 2 cut(s) 708, 812
SstI GAGCTC 1 cut(s) 725
TaaI ACNGT 2 cut(s) 481, 780
TaqI TCGA 7 cut(s) 189, 225, 506, 582, 719, 725, 736
TasI AATT 4 cut(s) 112, 133, 394, 440
TatI WGTACW 1 cut(s) 923
TfiI GAWTC 5 cut(s) 155, 231, 472, 650, 680
Tru1I TTAA 4 cut(s) 357, 446, 510, 570
Tru9I TTAA 4 cut(s) 357, 446, 510, 570
TscAI CASTG 1 cut(s) 486
TseI GCWGC 2 cut(s) 541, 1025
TspDTI ATGAA 7 cut(s) 77, 388, 396, 616, 648, 663, 672
TspGWI ACGGA 1 cut(s) 798
TspRI CASTG 1 cut(s) 486
Tth111I GACNNNGTC 1 cut(s) 578
Vha464I CTTAAG 1 cut(s) 356
XapI RAATTY 2 cut(s) 133, 394
XhoI CTCGAG 2 cut(s) 718, 724
XmnI GAANNNNTTC 3 cut(s) 623, 654, 679
XspI CTAG 2 cut(s) 708, 812
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.